DE69434358D1 - Methode zur herstellung der proteinase nla aus tabak - Google Patents

Methode zur herstellung der proteinase nla aus tabak

Info

Publication number
DE69434358D1
DE69434358D1 DE69434358T DE69434358T DE69434358D1 DE 69434358 D1 DE69434358 D1 DE 69434358D1 DE 69434358 T DE69434358 T DE 69434358T DE 69434358 T DE69434358 T DE 69434358T DE 69434358 D1 DE69434358 D1 DE 69434358D1
Authority
DE
Germany
Prior art keywords
plant virus
proteinase
proteins
nla
tobacco
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434358T
Other languages
English (en)
Inventor
Stephen A Johnston
William G Dougherty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oregon State University
University of Texas System
Original Assignee
Oregon State University
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oregon State University, University of Texas System filed Critical Oregon State University
Application granted granted Critical
Publication of DE69434358D1 publication Critical patent/DE69434358D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
DE69434358T 1993-02-12 1994-01-31 Methode zur herstellung der proteinase nla aus tabak Expired - Lifetime DE69434358D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/021,603 US5532142A (en) 1993-02-12 1993-02-12 Method of isolation and purification of fusion polypeptides
PCT/US1994/001176 WO1994018331A2 (en) 1993-02-12 1994-01-31 Fusion proteins including a cleavage site recognized by a plant virus protease

Publications (1)

Publication Number Publication Date
DE69434358D1 true DE69434358D1 (de) 2005-06-09

Family

ID=21805141

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434358T Expired - Lifetime DE69434358D1 (de) 1993-02-12 1994-01-31 Methode zur herstellung der proteinase nla aus tabak

Country Status (6)

Country Link
US (1) US5532142A (de)
EP (1) EP0682709B1 (de)
AT (1) ATE294870T1 (de)
AU (1) AU6132294A (de)
DE (1) DE69434358D1 (de)
WO (1) WO1994018331A2 (de)

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Also Published As

Publication number Publication date
WO1994018331A2 (en) 1994-08-18
ATE294870T1 (de) 2005-05-15
US5532142A (en) 1996-07-02
WO1994018331A3 (en) 1994-10-13
EP0682709B1 (de) 2005-05-04
AU6132294A (en) 1994-08-29
EP0682709A1 (de) 1995-11-22

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