DE69500303D1 - Methode zur sequenzierung kurzer oligonukleotide - Google Patents

Methode zur sequenzierung kurzer oligonukleotide

Info

Publication number: DE69500303D1
Authority: DE; Germany
Prior art keywords: oligonucleotide; primer; extension products; auxiliary; chain
Prior art date: 1994-01-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

DE69500303T

Other languages

English (en)

Other versions

DE69500303T2 (de

Inventor

Aharon Cohen

Alexei Belenky

Christopher Ott

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Aceragen Inc

Original Assignee

Hybridon Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-01-26

Filing date

1995-01-24

Publication date

1997-06-19

1995-01-24 Application filed by Hybridon Inc filed Critical Hybridon Inc

1997-06-19 Application granted granted Critical

1997-06-19 Publication of DE69500303D1 publication Critical patent/DE69500303D1/de

1997-10-30 Publication of DE69500303T2 publication Critical patent/DE69500303T2/de

2015-01-25 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 6
238000000034 method Methods 0.000 title abstract 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
238000012163 sequencing technique Methods 0.000 title 1
239000002777 nucleoside Substances 0.000 abstract 2
-1 nucleoside triphosphates Chemical class 0.000 abstract 2
239000001226 triphosphate Substances 0.000 abstract 2
235000011178 triphosphate Nutrition 0.000 abstract 2
230000000295 complement effect Effects 0.000 abstract 1
230000037230 mobility Effects 0.000 abstract 1
125000003835 nucleoside group Chemical group 0.000 abstract 1
239000002773 nucleotide Substances 0.000 abstract 1
125000003729 nucleotide group Chemical group 0.000 abstract 1
238000000926 separation method Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit

DE69500303T 1994-01-26 1995-01-24 Methode zur sequenzierung kurzer oligonukleotide Expired - Fee Related DE69500303T2 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US08/187,749 US5525470A (en)	1994-01-26	1994-01-26	Method of sequencing [short] oligonucleotides
PCT/US1995/001104 WO1995020680A1 (en)	1994-01-26	1995-01-24	A method of sequencing short oligonucleotides

Publications (2)

Publication Number	Publication Date
DE69500303D1 true DE69500303D1 (de)	1997-06-19
DE69500303T2 DE69500303T2 (de)	1997-10-30

Family

ID=22690308

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE69500303T Expired - Fee Related DE69500303T2 (de)	1994-01-26	1995-01-24	Methode zur sequenzierung kurzer oligonukleotide

Country Status (8)

Country	Link
US (1)	US5525470A (de)
EP (1)	EP0741799B1 (de)
JP (1)	JPH09508282A (de)
AT (1)	ATE153078T1 (de)
AU (1)	AU1734695A (de)
CA (1)	CA2180722A1 (de)
DE (1)	DE69500303T2 (de)
WO (1)	WO1995020680A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1997025444A1 (en) *	1996-01-16	1997-07-17	Hybridon, Inc.	Method of monitoring pharmacokinetics of oligonucleotide pharmaceuticals
US5851804A (en) *	1996-05-06	1998-12-22	Apollon, Inc.	Chimeric kanamycin resistance gene
US6291164B1 (en)	1996-11-22	2001-09-18	Invitrogen Corporation	Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
WO1998040520A1 (en) *	1997-03-14	1998-09-17	Hybridon, Inc.	Method for sequencing of modified nucleic acids using electrospray ionization-fourier transform mass spectrometry
US5888778A (en) *	1997-06-16	1999-03-30	Exact Laboratories, Inc.	High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US20040038206A1 (en) *	2001-03-14	2004-02-26	Jia Zhang	Method for high throughput assay of genetic analysis
JP2006500959A (ja) *	2002-09-30	2006-01-12	パラレルバイオサイエンス，インコーポレイテッド	動的サンプリング結合によるポリヌクレオチド合成および標識化
US20040219565A1 (en)	2002-10-21	2004-11-04	Sakari Kauppinen	Oligonucleotides useful for detecting and analyzing nucleic acids of interest
EP2530156B1 (de) *	2002-11-25	2015-11-18	Masafumi Matsuo	ENA-Nukleinsäurearzneimittel, die das Splicing im mRNA-Vorläufer verändern
US20090186343A1 (en) *	2003-01-28	2009-07-23	Visigen Biotechnologies, Inc.	Methods for preparing modified biomolecules, modified biomolecules and methods for using same
US8192937B2 (en) *	2004-04-07	2012-06-05	Exiqon A/S	Methods for quantification of microRNAs and small interfering RNAs
WO2006047787A2 (en)	2004-10-27	2006-05-04	Exact Sciences Corporation	Method for monitoring disease progression or recurrence
US9777314B2 (en)	2005-04-21	2017-10-03	Esoterix Genetic Laboratories, Llc	Analysis of heterogeneous nucleic acid samples
US20080045472A1 (en) *	2006-03-31	2008-02-21	Council Of Scientific And Industrial Research Bharat Biotech	Targets for human micro rnas in avian influenza virus (h5n1) genome
WO2020239822A1 (en) *	2019-05-27	2020-12-03	The European Molecular Biology Laboratory	Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0377015B1 (de) *	1988-05-24	1994-11-30	Gesellschaft für Biotechnologische Forschung mbH (GBF)	Oligonucleotidbank und verfahren zur dna-sequenzierung
US5403709A (en) *	1992-10-06	1995-04-04	Hybridon, Inc.	Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages

1994
- 1994-01-26 US US08/187,749 patent/US5525470A/en not_active Expired - Fee Related
1995
- 1995-01-24 CA CA002180722A patent/CA2180722A1/en not_active Abandoned
- 1995-01-24 WO PCT/US1995/001104 patent/WO1995020680A1/en active IP Right Grant
- 1995-01-24 DE DE69500303T patent/DE69500303T2/de not_active Expired - Fee Related
- 1995-01-24 AT AT95909358T patent/ATE153078T1/de active
- 1995-01-24 AU AU17346/95A patent/AU1734695A/en not_active Abandoned
- 1995-01-24 JP JP7520189A patent/JPH09508282A/ja active Pending
- 1995-01-24 EP EP95909358A patent/EP0741799B1/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
CA2180722A1 (en)	1995-08-03
ATE153078T1 (de)	1997-05-15
EP0741799A1 (de)	1996-11-13
DE69500303T2 (de)	1997-10-30
AU1734695A (en)	1995-08-15
US5525470A (en)	1996-06-11
JPH09508282A (ja)	1997-08-26
WO1995020680A1 (en)	1995-08-03
EP0741799B1 (de)	1997-05-14

Legal Events

Date	Code	Title	Description
1998-06-10	8364	No opposition during term of opposition
1999-02-18	8339	Ceased/non-payment of the annual fee

Publication	Publication Date	Title
DE69500303D1 (de)	1997-06-19	Methode zur sequenzierung kurzer oligonukleotide
DE69626111T2 (de)	2003-12-24	Universale primersequenz zur multiplex dna amplifikation
GB0021977D0 (en)	2000-10-25	Method of sequencing DNA
DE3850093T2 (de)	1994-11-03	Verfahren zur Amplifizierung von Nukleinsäuren.
DE60043182D1 (de)	2009-12-03	Methode zur dns-sequenzierung
WO1999009216A3 (en)	1999-05-06	Highly sensitive multimeric nucleic acid probes
ES2104611T3 (es)	1997-10-16	Procedimiento de amplificacion mejorado de acidos nucleicos.
WO2001071042A3 (en)	2003-03-13	Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
ATE288499T1 (de)	2005-02-15	Die verwendung von modularen oligonukleotiden als sonden oder primern in auf nukleinsaure basierenden tests
DE69815370D1 (de)	2003-07-10	Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
DK1171634T3 (da)	2006-03-20	Amplifikations- og sekventeringsprimerpar og anvendelse deraf
ATE279523T1 (de)	2004-10-15	Resistenz gegen nematoden und/oder blattläuse
ES2173897T3 (es)	2002-11-01	Procedimiento para la clonacion especifica de acidos nucleicos.
WO2002064822A3 (de)	2003-10-02	Verfahren und kit zur tierartspezifischen dna-identifikation in einer probe
DE60125054D1 (de)	2007-01-18	Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
DE60029351D1 (de)	2006-08-24	Einzelsträngige oligonukleotide, sonden, primer und ein vefahren zur detektion von spirochaeten
ATE483031T1 (de)	2010-10-15	Oligonukleotidprimer für das stn gen zur detektion von salmonella arten und entsprechende nachweismethoden
WO2003072814A3 (en)	2003-12-18	Improved method for allele-specific pcr
Johansson et al.	1993	Reverse transcription using nuclease resistant primers.
ATE404697T1 (de)	2008-08-15	Verfahren zum nachweis von genetischem polymorphismus
GB2302541A (en)	1997-01-22	Method of using mobile priming sites for DNA sequencing
TH17628EX (th)	1996-02-09	ก่อไพรเมอร์ที่ไม่เป็นการเฉพาะในการตรวจหา dna และชุดสำหรับตรวจหานิวคลีอิคเป้าหมาย ด้วยโอลิโกนิวคลีโอไทด์ที่บล็อค
TH28996B (th)	1998-06-25	"โอลิโกนิวคลีโอไทด์จำเพาะสำหรับไวรัสตับอักเสบ c