DE69535882D1 - Fluoreszenztest zum nachweis der spaltung einer nukleinsäure - Google Patents

Fluoreszenztest zum nachweis der spaltung einer nukleinsäure

Info

Publication number
DE69535882D1
DE69535882D1 DE69535882T DE69535882T DE69535882D1 DE 69535882 D1 DE69535882 D1 DE 69535882D1 DE 69535882 T DE69535882 T DE 69535882T DE 69535882 T DE69535882 T DE 69535882T DE 69535882 D1 DE69535882 D1 DE 69535882D1
Authority
DE
Germany
Prior art keywords
cleavage
detecting
nucleic acid
fluorescent test
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69535882T
Other languages
English (en)
Inventor
Myun Ki Han
S Paul Lee
Jack G Chirikjian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Georgetown University
Original Assignee
Georgetown University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georgetown University filed Critical Georgetown University
Application granted granted Critical
Publication of DE69535882D1 publication Critical patent/DE69535882D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
DE69535882T 1994-12-30 1995-12-27 Fluoreszenztest zum nachweis der spaltung einer nukleinsäure Expired - Lifetime DE69535882D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36547394A 1994-12-30 1994-12-30
PCT/US1995/016904 WO1996021144A1 (en) 1994-12-30 1995-12-27 Fluorometric assay for detecting nucleic acid cleavage

Publications (1)

Publication Number Publication Date
DE69535882D1 true DE69535882D1 (de) 2008-12-18

Family

ID=23439046

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69535882T Expired - Lifetime DE69535882D1 (de) 1994-12-30 1995-12-27 Fluoreszenztest zum nachweis der spaltung einer nukleinsäure

Country Status (5)

Country Link
US (1) US5763181A (de)
EP (1) EP0803058B1 (de)
CA (1) CA2209080C (de)
DE (1) DE69535882D1 (de)
WO (1) WO1996021144A1 (de)

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US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US6459805B1 (en) * 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725237D0 (en) † 1997-11-29 1998-01-28 Secr Defence Amplification system
JP4388694B2 (ja) * 1998-02-04 2009-12-24 アプライド バイオシステムズ， エルエルシー 複数のアレル部位における増幅産物のゲノム型決定
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU765428B2 (en) * 1998-03-13 2003-09-18 Promega Corporation Methods for determining the presence of nucleic acid target sequences and applications thereof
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6277578B1 (en) * 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6335162B1 (en) 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
CA2357011A1 (en) * 1999-02-18 2000-08-24 Promega Corporation Multiplex method for nucleic acid detection
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US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6844154B2 (en) * 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
EP1328658A2 (de) * 2000-06-22 2003-07-23 San Diego State University Foundation Rekombinationsmodulatoren und verfahren zu ihrer herstellung und verwendung
US20020187484A1 (en) * 2000-11-27 2002-12-12 Jon Thorson Molecular break lights probes for detecting nucleotide cleavage
WO2002095002A2 (en) 2001-05-22 2002-11-28 University Of Chicago N4 virion single-stranded dna dependent rna polymerase
US6544746B2 (en) * 2001-08-13 2003-04-08 St. Louis University Rapid and sensitive proximity-based assay for the detection and quantification of DNA binding proteins
US7172865B2 (en) 2001-08-13 2007-02-06 Saint Louis University Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
WO2003064679A2 (en) 2002-01-30 2003-08-07 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
FR2836926A1 (fr) * 2002-06-14 2003-09-12 Commissariat Energie Atomique Substrat oligonucleotidique et procede permettant d'analyser la presence d'activites de reparation de lesions de l'adn
US7785782B2 (en) * 2002-12-12 2010-08-31 Novartis Vaccines And Diagnostics, Inc. Device and method for in-line blood testing using biochips
KR101041106B1 (ko) * 2003-11-25 2011-06-13 한면기 핵산과 단백질의 신규한 실시간 탐지방법
WO2006088910A2 (en) * 2005-02-15 2006-08-24 Georgetown University Sequence-specific detection of nucleotide sequences
US20090111104A1 (en) * 2005-11-04 2009-04-30 Fluorotechnics Pty Limited Method for Monitoring Hydrolytic Activity
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2401388A4 (de) 2009-02-23 2012-12-05 Univ Georgetown Sequenzspezifischer nachweis von nukleotidsequenzen
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
KR20120046018A (ko) * 2010-10-04 2012-05-09 삼성테크윈 주식회사 단일 뉴클레오티드 다형성의 실시간 ｐｃｒ 검출
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
CN102321759B (zh) * 2011-08-25 2013-05-08 南京邮电大学 一种检测s1核酸酶及其抑制剂的荧光方法
WO2013081206A1 (ko) * 2011-11-30 2013-06-06 삼성테크윈 주식회사 안정화된 프로브를 이용한 실시간 ｐｃｒ 검출
CA3207128A1 (en) 2013-11-14 2015-05-21 Integrated Dna Technologies, Inc. Dna polymerase mutants having enhanced template discrimination activity
US11060149B2 (en) 2014-06-18 2021-07-13 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
AU2016369603A1 (en) 2015-12-18 2018-07-05 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
WO2017142297A1 (ko) 2016-02-15 2017-08-24 주식회사 누리바이오 실시간 핵산 또는 단백질 검출용 단일핵산 및 이를 이용한 검출 방법
KR101901749B1 (ko) 2016-02-15 2018-09-28 주식회사 누리바이오 실시간 핵산 또는 단백질 검출용 단일핵산 및 이를 이용한 검출 방법
CN109476695A (zh) 2016-06-27 2019-03-15 丹娜法伯癌症研究院 用于测定rna翻译速率的方法
CN110023507B (zh) * 2016-09-27 2024-01-23 纽丽生物科技有限公司 检测小rna或与小rna相关的蛋白质的方法
AU2022305653A1 (en) 2021-06-30 2024-01-18 Dana-Farber Cancer Institute, Inc. Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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JP2856804B2 (ja) * 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
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US5001769A (en) * 1988-12-20 1991-03-19 Educational Testing Service Image processing system
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase

Also Published As

Publication number Publication date
EP0803058A4 (de) 2001-08-16
CA2209080A1 (en) 1996-07-11
WO1996021144A1 (en) 1996-07-11
EP0803058B1 (de) 2008-11-05
EP0803058A1 (de) 1997-10-29
US5763181A (en) 1998-06-09
CA2209080C (en) 2008-07-29

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