DE69732360D1 - Plasmide dna reinigung durch peg-fällung und säulen-chromatographie - Google Patents

Plasmide dna reinigung durch peg-fällung und säulen-chromatographie

Info

Publication number
DE69732360D1
DE69732360D1 DE69732360T DE69732360T DE69732360D1 DE 69732360 D1 DE69732360 D1 DE 69732360D1 DE 69732360 T DE69732360 T DE 69732360T DE 69732360 T DE69732360 T DE 69732360T DE 69732360 D1 DE69732360 D1 DE 69732360D1
Authority
DE
Germany
Prior art keywords
column chromatography
plasmid dna
dna
dna cleaning
peg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69732360T
Other languages
English (en)
Other versions
DE69732360T2 (de
Inventor
Nancy Horn
Greg Budahazi
Magda Marquet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of DE69732360D1 publication Critical patent/DE69732360D1/de
Publication of DE69732360T2 publication Critical patent/DE69732360T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DE69732360T 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie Expired - Lifetime DE69732360T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US692590 1996-08-06
US08/692,590 US5707812A (en) 1996-08-06 1996-08-06 Purification of plasmid DNA during column chromatography
PCT/US1997/013647 WO1998005767A1 (en) 1996-08-06 1997-08-05 Purification of plasmid dna by peg-precipitation and column chromatography

Publications (2)

Publication Number Publication Date
DE69732360D1 true DE69732360D1 (de) 2005-03-03
DE69732360T2 DE69732360T2 (de) 2006-04-06

Family

ID=24781207

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69732360T Expired - Lifetime DE69732360T2 (de) 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie

Country Status (7)

Country Link
US (1) US5707812A (de)
EP (1) EP0920497B1 (de)
JP (1) JP3905132B2 (de)
AT (1) ATE287948T1 (de)
CA (1) CA2262820C (de)
DE (1) DE69732360T2 (de)
WO (1) WO1998005767A1 (de)

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US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6348450B1 (en) 1997-08-13 2002-02-19 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom and uses thereof
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6706693B1 (en) 1997-08-13 2004-03-16 The Uab Research Foundation Vaccination by topical application of genetic vectors
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
US6111096A (en) 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
EP0964057B1 (de) 1998-05-11 2004-12-22 Tosoh Corporation Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
DE19916534A1 (de) * 1999-04-13 2000-10-19 Gsf Forschungszentrum Umwelt RNA-Isolierungs-Kit
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
CZ20014274A3 (cs) 1999-05-28 2003-01-15 Cambrex Bio Science Baltimore, Inc. Způsob čiątění DNA a vyčiątěná DNA
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020010145A1 (en) * 1999-07-12 2002-01-24 Willson Richard C. Apparatus, methods and compositions for biotechnical separations
US6313285B1 (en) 1999-07-23 2001-11-06 Genentech, Inc. Purification of plasmid DNA
US6281349B1 (en) 1999-08-18 2001-08-28 David E. Pulleyblank Method for nucleic acid purification using iodine
US6464976B1 (en) 1999-09-07 2002-10-15 Canji, Inc. Methods and compositions for reducing immune response
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
PT102491B (pt) 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7074333B2 (en) * 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
KR100502473B1 (ko) * 2001-11-08 2005-07-20 동아제약주식회사 플라스미드 ｄｎａ의 분리방법
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
US20040016702A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
DK2364769T3 (en) 2003-05-30 2016-04-11 Vgxi Inc Apparatus and methods for biomaterialeproduktion
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US7504243B2 (en) 2004-03-19 2009-03-17 The United States Of America As Represented By The Department Of Health And Human Services Methods for the production of biliverdin
EP1776458A2 (de) 2004-07-30 2007-04-25 Agencourt Bioscience Corporation Verfahren zur isolierung von nukleinsäuren mit hilfe von festphasenträgern mit multifunktionalen gruppen
EP1856262B1 (de) 2005-01-31 2012-08-15 Merck Sharp & Dohme Corp. Upstream- und downstream-reinigungsverfahren zur massenfertigung von plasmider dna
CA2597482A1 (en) 2005-02-11 2006-08-17 Invitrogen Dynal As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
AU2006216671A1 (en) * 2005-02-23 2006-08-31 The Uab Research Foundation Alkyl-glycoside enhanced vaccination
WO2006113209A1 (en) * 2005-04-15 2006-10-26 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen
US7960105B2 (en) * 2005-11-29 2011-06-14 National Institutes Of Health Method of DNA analysis using micro/nanochannel
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
US8067580B2 (en) * 2008-02-07 2011-11-29 Ge Healthcare Bio-Sciences Corp. Isolation of DNA, RNA and protein from a single sample
WO2009111336A2 (en) * 2008-02-29 2009-09-11 Shizhong Chen Methods of purifying plasmid dna
CA2887341C (en) 2012-10-12 2021-03-16 Sage Science, Inc. Side-eluting molecular fractionator
EP3447141B1 (de) 2013-03-18 2020-08-05 PreAnalytiX GmbH Stabilisierung von biologischen proben
WO2016061416A1 (en) 2014-10-15 2016-04-21 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US20170239658A1 (en) * 2014-10-16 2017-08-24 Ezra S. Abrams Methods and systems for controlling the shear modulus of genomic length dsdna molecules
EP3414255B1 (de) 2015-11-20 2021-09-22 Washington University Präparatives elektrophoretisches verfahren zur gezielten reinigung genomischer dna-fragmente
JP2020515250A (ja) 2017-04-07 2020-05-28 セージ サイエンス， インコーポレイテッド 統合型電気泳動ｄｎａ精製を使用する遺伝的構造変動を検出するためのシステムおよび方法
KR20220029556A (ko) * 2019-05-15 2022-03-08 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
CN115612684A (zh) * 2022-11-01 2023-01-17 北京启辰生生物科技有限公司 Gmp级质粒dna大规模简化生产方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01304886A (ja) * 1988-05-31 1989-12-08 Sekisui Chem Co Ltd Ｄｎａの精製方法
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
US5707812A (en) 1998-01-13
JP3905132B2 (ja) 2007-04-18
DE69732360T2 (de) 2006-04-06
EP0920497A1 (de) 1999-06-09
EP0920497B1 (de) 2005-01-26
WO1998005767A1 (en) 1998-02-12
CA2262820A1 (en) 1998-02-12
CA2262820C (en) 2007-10-23
ATE287948T1 (de) 2005-02-15
JP2000515385A (ja) 2000-11-21

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