DE69734913D1 - Verfahren für reverse Transkription - Google Patents

Verfahren für reverse Transkription

Info

Publication number
DE69734913D1
DE69734913D1 DE69734913T DE69734913T DE69734913D1 DE 69734913 D1 DE69734913 D1 DE 69734913D1 DE 69734913 T DE69734913 T DE 69734913T DE 69734913 T DE69734913 T DE 69734913T DE 69734913 D1 DE69734913 D1 DE 69734913D1
Authority
DE
Germany
Prior art keywords
reverse transcription
transcription method
reverse
transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69734913T
Other languages
English (en)
Other versions
DE69734913T2 (de
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute Of Physical & Chemical Research Wak
Dnaform KK
Original Assignee
Institute Of Physical & Chemical Research Wako
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP19633196A external-priority patent/JP3709490B2/ja
Application filed by Institute Of Physical & Chemical Research Wako, RIKEN Institute of Physical and Chemical Research filed Critical Institute Of Physical & Chemical Research Wako
Publication of DE69734913D1 publication Critical patent/DE69734913D1/de
Application granted granted Critical
Publication of DE69734913T2 publication Critical patent/DE69734913T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
DE69734913T 1996-07-25 1997-07-24 Verfahren für reverse Transkription Expired - Lifetime DE69734913T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP19632996 1996-07-25
JP19633196A JP3709490B2 (ja) 1996-07-25 1996-07-25 Ｒｎａの熱安定性の改良方法
JP19632996 1996-07-25
JP19633196 1996-07-25

Publications (2)

Publication Number Publication Date
DE69734913D1 true DE69734913D1 (de) 2006-01-26
DE69734913T2 DE69734913T2 (de) 2006-08-24

Family

ID=26509679

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69734913T Expired - Lifetime DE69734913T2 (de) 1996-07-25 1997-07-24 Verfahren für reverse Transkription
DE69738354T Expired - Lifetime DE69738354D1 (de) 1996-07-25 1997-07-24 Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69738354T Expired - Lifetime DE69738354D1 (de) 1996-07-25 1997-07-24 Verwendung bestimmter Substanzen für das Verbessern der Wärmestabilität von RNS

Country Status (4)

Country Link
US (3) US6013488A (de)
EP (2) EP1243661B1 (de)
CA (1) CA2211367C (de)
DE (2) DE69734913T2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2287542A1 (en) 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
AU8162598A (en) * 1997-06-25 1999-01-04 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
EP1129064B1 (de) 1998-11-12 2008-01-09 Invitrogen Corporation Transportreagentien
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
WO2000055307A2 (en) * 1999-03-12 2000-09-21 Mcgill University A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
DE10105208A1 (de) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Verfahren zur Herstellung von cDNA
ES2188357B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio.
US20050250100A1 (en) * 2002-06-12 2005-11-10 Yoshihide Hayashizaki Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
WO2005035569A2 (en) * 2003-10-10 2005-04-21 Five Prime Therapeutics, Inc. Kiaa0779, splice variants thereof, and methods of their use
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
CN101035620B (zh) 2004-04-08 2012-01-04 生物马特里卡公司 用于生命科学的样品存储和样品管理的综合
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US7993853B2 (en) 2005-05-06 2011-08-09 Gen-Probe Incorporated Methods of nucleic acid target capture
EP2405024A1 (de) 2005-07-15 2012-01-11 Life Technologies Corporation Heissstart reverse Transkription durch primer design
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
FR2984357B1 (fr) * 2011-12-16 2016-11-18 Biomerieux Sa Procede d'amplification transcriptionnelle d'acides nucleiques reunissant des etapes de temperatures differentes
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
WO2015112767A2 (en) 2014-01-22 2015-07-30 Life Technologies Corporation Novel reverse transcriptases for use in high temperature nucleic acid synthesis
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
EP3169310A1 (de) 2014-07-15 2017-05-24 Life Technologies Corporation Zusammensetzungen mit lipidaggregaten und verfahren zur effizienten bereitstellung von molekülen an zellen
WO2017100212A1 (en) 2015-12-08 2017-06-15 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
US11312991B2 (en) 2016-01-27 2022-04-26 Kabushiki Kaisha Dnaform Method for decoding base sequence of nucleic acid corresponding to end region of RNA and method for analyzing DNA element

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035204B2 (de) * 1980-03-05 1991-05-22 Miles Inc. Pasteurisierte therapeutisch aktive Proteinzusammensetzungen
US4409200A (en) * 1980-04-28 1983-10-11 Research Corporation Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
EP0506889B1 (de) * 1989-12-22 1997-04-02 F. Hoffmann-La Roche AG Bei hoher temperatur aktive reverse transkriptasen
DE4124286A1 (de) * 1991-07-22 1993-01-28 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von proteinen bei optischen tests
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
CA2141430C (en) * 1992-08-04 2009-05-12 Sherrol H. Mcdonough Nucleic acid sequence amplification
WO1994012657A1 (en) * 1992-11-20 1994-06-09 Katcher Harold L Direct-phenol pcr, rt and rt-pcr methods
DE4239969A1 (de) * 1992-11-27 1994-06-01 Boehringer Mannheim Gmbh Verfahren zur Stabilisierung von Proteinen
EP0655091A1 (de) * 1993-05-06 1995-05-31 Baxter Diagnostics Inc. Nachweismethode des menschlichen papillomavirus
WO1995014770A1 (en) * 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Improved polymerase
DE69531487T2 (de) * 1994-01-31 2004-06-17 The Regents Of The University Of California, Oakland Methode zur eliminierung von sequenzierartefakten
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Also Published As

Publication number Publication date
US20010012617A1 (en) 2001-08-09
CA2211367A1 (en) 1998-01-25
US6013488A (en) 2000-01-11
DE69734913T2 (de) 2006-08-24
DE69738354D1 (de) 2008-01-17
EP0821059A2 (de) 1998-01-28
EP0821059B8 (de) 2006-03-08
EP0821059B1 (de) 2005-12-21
EP1243661A1 (de) 2002-09-25
CA2211367C (en) 2008-04-22
US6372437B2 (en) 2002-04-16
US6221599B1 (en) 2001-04-24
EP0821059A3 (de) 1998-04-22
EP1243661B1 (de) 2007-12-05

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH, WAK

Owner name: KABUSHIKI KAISHA DNAFORM, TOKIO/TOKYO, JP

8364 No opposition during term of opposition