DE69826465D1 - Verfahren zur in vitro evolution von proteinfunktionen - Google Patents
Verfahren zur in vitro evolution von proteinfunktionenInfo
- Publication number
- DE69826465D1 DE69826465D1 DE69826465T DE69826465T DE69826465D1 DE 69826465 D1 DE69826465 D1 DE 69826465D1 DE 69826465 T DE69826465 T DE 69826465T DE 69826465 T DE69826465 T DE 69826465T DE 69826465 D1 DE69826465 D1 DE 69826465D1
- Authority
- DE
- Germany
- Prior art keywords
- vitro evolution
- protein functions
- polynucleotide sequence
- relates
- exonuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9712512.4A GB9712512D0 (en) | 1997-06-16 | 1997-06-16 | A method for in vitro molecular evolution of protein function |
GB9712512 | 1997-06-16 | ||
PCT/GB1998/001757 WO1998058080A1 (en) | 1997-06-16 | 1998-06-16 | A method for in vitro molecular evolution of protein function |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69826465D1 true DE69826465D1 (de) | 2004-10-28 |
DE69826465T2 DE69826465T2 (de) | 2005-09-29 |
Family
ID=10814243
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69826465T Expired - Fee Related DE69826465T2 (de) | 1997-06-16 | 1998-06-16 | Verfahren zur in vitro evolution von proteinfunktionen |
Country Status (12)
Country | Link |
---|---|
US (2) | US6495321B1 (de) |
EP (1) | EP0990044B1 (de) |
JP (1) | JP3486676B2 (de) |
AT (1) | ATE277198T1 (de) |
AU (1) | AU745150B2 (de) |
CA (1) | CA2293819C (de) |
DE (1) | DE69826465T2 (de) |
DK (1) | DK0990044T3 (de) |
ES (1) | ES2232953T3 (de) |
GB (1) | GB9712512D0 (de) |
PT (1) | PT990044E (de) |
WO (1) | WO1998058080A1 (de) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6939689B2 (en) | 1995-12-07 | 2005-09-06 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
US6352842B1 (en) | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US6713279B1 (en) | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US7018793B1 (en) | 1995-12-07 | 2006-03-28 | Diversa Corporation | Combinatorial screening of mixed populations of organisms |
ES2373110T3 (es) | 1997-01-21 | 2012-01-31 | The General Hospital Corporation | Selección de proteínas usando fusiones de arn-proteína. |
US6261804B1 (en) | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
US8207093B2 (en) | 1997-01-21 | 2012-06-26 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
US6951719B1 (en) * | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
DE69941193D1 (de) | 1998-08-17 | 2009-09-10 | Bristol Myers Squibb Co | Identifizierung von verbindung-protein wechselwirkungen mit bibliotheken von gekoppelten protein-nukleinsäure-molekülen |
US6438561B1 (en) * | 1998-11-19 | 2002-08-20 | Navigation Technologies Corp. | Method and system for using real-time traffic broadcasts with navigation systems |
US6917882B2 (en) * | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
WO2000042559A1 (en) * | 1999-01-18 | 2000-07-20 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
ATE465459T1 (de) | 1999-01-19 | 2010-05-15 | Maxygen Inc | Durch oligonukleotide-vermittelte nukleinsäuren- rekombination |
US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
US7024312B1 (en) * | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US20070065838A1 (en) * | 1999-01-19 | 2007-03-22 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US8457903B1 (en) | 1999-01-19 | 2013-06-04 | Codexis Mayflower Holdings, Llc | Method and/or apparatus for determining codons |
US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
US7873477B1 (en) | 2001-08-21 | 2011-01-18 | Codexis Mayflower Holdings, Llc | Method and system using systematically varied data libraries |
AU3879300A (en) * | 1999-03-09 | 2000-09-28 | Diversa Corporation | End selection in directed evolution |
JP2004500019A (ja) * | 1999-03-26 | 2004-01-08 | ディベルサ コーポレーション | 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合 |
US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
US7430477B2 (en) * | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
DE19953854C2 (de) * | 1999-11-09 | 2002-01-17 | Max Planck Gesellschaft | Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften |
US7022479B2 (en) | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
WO2001053539A1 (en) | 2000-01-24 | 2001-07-26 | Phylos, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
US6479262B1 (en) * | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
US6994963B1 (en) | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
AUPQ974900A0 (en) | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
US6993458B1 (en) * | 2000-11-07 | 2006-01-31 | International Business Machines Corporation | Method and apparatus for preprocessing technique for forecasting in capacity management, software rejuvenation and dynamic resource allocation applications |
US6958213B2 (en) * | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
AU3457202A (en) * | 2000-12-12 | 2002-06-24 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
GB2370038B (en) * | 2000-12-12 | 2003-03-26 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US7402383B2 (en) * | 2001-07-23 | 2008-07-22 | Dsm Ip Assets B.V. | Process for preparing variant polynucleotides |
WO2003010311A2 (en) * | 2001-07-23 | 2003-02-06 | Dsm Ip Assets B.V. | Process for preparing variant polynucleotides |
AU2002365811A1 (en) * | 2001-12-03 | 2003-06-17 | Zymogenetics, Inc. | Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations |
EP1501927A2 (de) * | 2002-05-02 | 2005-02-02 | University Of North Carolina At Chapel Hill | In-vitro-mutagenese, phänotypisierung und genkartierung |
GB2388604B (en) * | 2002-05-17 | 2005-01-26 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
DK1504098T4 (da) * | 2002-05-17 | 2011-05-23 | Alligator Bioscience Ab | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
US20060134624A1 (en) * | 2003-02-06 | 2006-06-22 | Salerno John C | Polymerase-based protocols for the introductions of deletions and insertions |
US20060257876A1 (en) * | 2003-02-06 | 2006-11-16 | Rensselaer Polytechnis Institute | Polymerase-based protocols for generating chimeric and combinatorial... |
US20060228786A1 (en) * | 2003-02-06 | 2006-10-12 | Salerno John C | Polymerase-based protocols for the introduction of deletions and insertions |
EP1626978A4 (de) * | 2003-02-06 | 2007-05-02 | Rensselaer Polytech Inst | Auf polymerase basierende protokolle zur einführung von deletionen und insertionen |
GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
GB0607798D0 (en) | 2006-04-20 | 2006-05-31 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
EP2130918A1 (de) | 2008-06-05 | 2009-12-09 | C-Lecta GmbH | Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen |
US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
GB0905790D0 (en) | 2009-04-03 | 2009-05-20 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
DE102010056289A1 (de) * | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
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US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
WO1990007936A1 (en) | 1989-01-23 | 1990-07-26 | Chiron Corporation | Recombinant therapies for infection and hyperproliferative disorders |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
US5023171A (en) | 1989-08-10 | 1991-06-11 | Mayo Foundation For Medical Education And Research | Method for gene splicing by overlap extension using the polymerase chain reaction |
GB8919607D0 (en) | 1989-08-30 | 1989-10-11 | Wellcome Found | Novel entities for cancer therapy |
WO1991006570A1 (en) | 1989-10-25 | 1991-05-16 | The University Of Melbourne | HYBRID Fc RECEPTOR MOLECULES |
US5573907A (en) * | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
US5858725A (en) | 1990-10-10 | 1999-01-12 | Glaxo Wellcome Inc. | Preparation of chimaeric antibodies using the recombinant PCR strategy |
WO1993000103A1 (en) | 1991-06-21 | 1993-01-07 | The Wistar Institute Of Anatomy And Biology | Chimeric envelope proteins for viral targeting |
GB9115364D0 (en) | 1991-07-16 | 1991-08-28 | Wellcome Found | Antibody |
DE69229477T2 (de) | 1991-09-23 | 1999-12-09 | Cambridge Antibody Tech | Methoden zur Herstellung humanisierter Antikörper |
IL103059A0 (en) | 1991-09-30 | 1993-02-21 | Boehringer Ingelheim Int | Conjugates for introducing nucleic acid into higher eucaryotic cells |
US5252479A (en) | 1991-11-08 | 1993-10-12 | Research Corporation Technologies, Inc. | Safe vector for gene therapy |
GB9125979D0 (en) | 1991-12-06 | 1992-02-05 | Wellcome Found | Antibody |
JP3431146B2 (ja) | 1992-01-30 | 2003-07-28 | ジェンザイム・リミテッド | 改変した酵素によるキラル合成 |
GB9202796D0 (en) | 1992-02-11 | 1992-03-25 | Wellcome Found | Antiviral antibody |
JP3507073B2 (ja) | 1992-03-24 | 2004-03-15 | ケンブリッジ アンティボディー テクノロジー リミティド | 特異的結合対の成員の製造方法 |
WO1993025237A1 (en) | 1992-06-15 | 1993-12-23 | City Of Hope | Chimeric anti-cea antibody |
US6107062A (en) | 1992-07-30 | 2000-08-22 | Inpax, Inc. | Antisense viruses and antisense-ribozyme viruses |
US5837821A (en) | 1992-11-04 | 1998-11-17 | City Of Hope | Antibody construct |
CA2150262C (en) | 1992-12-04 | 2008-07-08 | Kaspar-Philipp Holliger | Multivalent and multispecific binding proteins, their manufacture and use |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
-
1997
- 1997-06-16 GB GBGB9712512.4A patent/GB9712512D0/en active Pending
-
1998
- 1998-06-16 PT PT98930871T patent/PT990044E/pt unknown
- 1998-06-16 DE DE69826465T patent/DE69826465T2/de not_active Expired - Fee Related
- 1998-06-16 EP EP98930871A patent/EP0990044B1/de not_active Revoked
- 1998-06-16 CA CA002293819A patent/CA2293819C/en not_active Expired - Fee Related
- 1998-06-16 US US09/445,649 patent/US6495321B1/en not_active Expired - Lifetime
- 1998-06-16 DK DK98930871T patent/DK0990044T3/da active
- 1998-06-16 AT AT98930871T patent/ATE277198T1/de not_active IP Right Cessation
- 1998-06-16 WO PCT/GB1998/001757 patent/WO1998058080A1/en not_active Application Discontinuation
- 1998-06-16 ES ES98930871T patent/ES2232953T3/es not_active Expired - Lifetime
- 1998-06-16 US US09/098,287 patent/US6159690A/en not_active Expired - Lifetime
- 1998-06-16 AU AU81159/98A patent/AU745150B2/en not_active Ceased
- 1998-06-16 JP JP50396899A patent/JP3486676B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU745150B2 (en) | 2002-03-14 |
DE69826465T2 (de) | 2005-09-29 |
DK0990044T3 (da) | 2004-12-06 |
US6159690A (en) | 2000-12-12 |
JP2002505584A (ja) | 2002-02-19 |
US6495321B1 (en) | 2002-12-17 |
ES2232953T3 (es) | 2005-06-01 |
AU8115998A (en) | 1999-01-04 |
PT990044E (pt) | 2005-01-31 |
JP3486676B2 (ja) | 2004-01-13 |
CA2293819C (en) | 2006-10-31 |
CA2293819A1 (en) | 1998-12-23 |
EP0990044B1 (de) | 2004-09-22 |
WO1998058080A1 (en) | 1998-12-23 |
GB9712512D0 (en) | 1997-08-20 |
EP0990044A1 (de) | 2000-04-05 |
ATE277198T1 (de) | 2004-10-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8363 | Opposition against the patent | ||
8339 | Ceased/non-payment of the annual fee |