DE69837208D1 - Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben - Google Patents
Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselbenInfo
- Publication number
- DE69837208D1 DE69837208D1 DE69837208T DE69837208T DE69837208D1 DE 69837208 D1 DE69837208 D1 DE 69837208D1 DE 69837208 T DE69837208 T DE 69837208T DE 69837208 T DE69837208 T DE 69837208T DE 69837208 D1 DE69837208 D1 DE 69837208D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- library
- libraries
- acid molecules
- normalized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/08—Methods of screening libraries by measuring catalytic activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5981797P | 1997-09-24 | 1997-09-24 | |
| US59817P | 1997-09-24 | ||
| US09/159,496 US6399334B1 (en) | 1997-09-24 | 1998-09-23 | Normalized nucleic acid libraries and methods of production thereof |
| US159496 | 1998-09-23 | ||
| PCT/US1998/019948 WO1999015702A1 (en) | 1997-09-24 | 1998-09-24 | Normalized nucleic acid libraries and methods of production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69837208D1 true DE69837208D1 (de) | 2007-04-12 |
| DE69837208T2 DE69837208T2 (de) | 2007-11-22 |
Family
ID=26739222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69837208T Expired - Lifetime DE69837208T2 (de) | 1997-09-24 | 1998-09-24 | Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US6399334B1 (de) |
| EP (1) | EP1017853B1 (de) |
| JP (1) | JP2001517460A (de) |
| AT (1) | ATE355367T1 (de) |
| AU (1) | AU9505998A (de) |
| CA (1) | CA2304895A1 (de) |
| DE (1) | DE69837208T2 (de) |
| ES (1) | ES2281138T3 (de) |
| WO (1) | WO1999015702A1 (de) |
Families Citing this family (95)
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| DE69825601T2 (de) * | 1997-02-12 | 2005-04-28 | Chan, Eugene Y, Brookline | Verfahren zur analyse von polymeren |
| US6399334B1 (en) * | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
| US6638717B2 (en) | 1999-05-19 | 2003-10-28 | Aventis Pharmaceuticals, Inc. | Microarray-based subtractive hybridzation |
| NZ515673A (en) * | 1999-05-21 | 2004-03-26 | Invitrogen Corp | Compositions and methods for labeling of nucleic acid molecules |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| JP2004511211A (ja) | 2000-05-26 | 2004-04-15 | インヴィトロジェン コーポレーション | 耐熱性逆転写酵素およびその使用 |
| US7078208B2 (en) | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
| EP1395678A2 (de) * | 2000-08-07 | 2004-03-10 | Genemed Biotechnologies Inc. | Methode zur identifizierung von in geringen mengen vorhandenen nukleinsäuresequenzen und verwandte zusammensetzungen |
| DE60124363T2 (de) * | 2000-08-25 | 2007-09-06 | Riken, Wako | Methode zur Herstellung von genormten und/oder subtrahierten cDNA |
| JP2004229501A (ja) * | 2000-10-30 | 2004-08-19 | Nippon Shinyaku Co Ltd | 臓器別又は組織別の平均化ｃＤＮＡライブラリーを用いて作製したＤＮＡアレイ |
| EP1354064A2 (de) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen |
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| US8152810B2 (en) | 2004-11-23 | 2012-04-10 | Jackson Roger P | Spinal fixation tool set and method |
| US8066739B2 (en) | 2004-02-27 | 2011-11-29 | Jackson Roger P | Tool system for dynamic spinal implants |
| AU2004317551B2 (en) | 2004-02-27 | 2008-12-04 | Roger P. Jackson | Orthopedic implant rod reduction tool set and method |
| US7789896B2 (en) * | 2005-02-22 | 2010-09-07 | Jackson Roger P | Polyaxial bone screw assembly |
| EP1737878A2 (de) | 2004-04-05 | 2007-01-03 | Alnylam Pharmaceuticals Inc. | Verfahren und reagentien für die synthese und aufreinigung von oligonukleotiden |
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| EP1789553B1 (de) | 2004-06-30 | 2014-03-26 | Alnylam Pharmaceuticals Inc. | Oligonukleotide mit einer nicht-phosphat-rückgratbindung |
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| US5726022A (en) | 1996-11-18 | 1998-03-10 | Lifespan Biosciences, Inc. | Subtractive hybridization and capture methods and kits for differential isolation of nucleic acids including disease-associated sequences |
| US6060245A (en) * | 1996-12-13 | 2000-05-09 | Stratagene | Methods and adaptors for generating specific nucleic acid populations |
| WO1998059075A1 (en) * | 1997-06-25 | 1998-12-30 | Life Technologies, Inc. | Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence |
| US6399334B1 (en) * | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
| WO1999045154A1 (en) * | 1998-03-04 | 1999-09-10 | Diversa Corporation | Production and use of normalized dna libraries |
-
1998
- 1998-09-23 US US09/159,496 patent/US6399334B1/en not_active Expired - Lifetime
- 1998-09-24 AT AT98948499T patent/ATE355367T1/de not_active IP Right Cessation
- 1998-09-24 WO PCT/US1998/019948 patent/WO1999015702A1/en active IP Right Grant
- 1998-09-24 DE DE69837208T patent/DE69837208T2/de not_active Expired - Lifetime
- 1998-09-24 ES ES98948499T patent/ES2281138T3/es not_active Expired - Lifetime
- 1998-09-24 CA CA002304895A patent/CA2304895A1/en not_active Abandoned
- 1998-09-24 EP EP98948499A patent/EP1017853B1/de not_active Expired - Lifetime
- 1998-09-24 JP JP2000512989A patent/JP2001517460A/ja not_active Withdrawn
- 1998-09-24 AU AU95059/98A patent/AU9505998A/en not_active Abandoned
-
2002
- 2002-05-13 US US10/143,007 patent/US20020177155A1/en not_active Abandoned
-
2006
- 2006-04-14 US US11/404,122 patent/US20060269940A1/en not_active Abandoned
-
2007
- 2007-09-06 US US11/851,353 patent/US20090264304A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE355367T1 (de) | 2006-03-15 |
| CA2304895A1 (en) | 1999-04-01 |
| EP1017853B1 (de) | 2007-02-28 |
| US20020034790A1 (en) | 2002-03-21 |
| EP1017853A4 (de) | 2004-11-17 |
| DE69837208T2 (de) | 2007-11-22 |
| US20090264304A1 (en) | 2009-10-22 |
| AU9505998A (en) | 1999-04-12 |
| US20020177155A1 (en) | 2002-11-28 |
| EP1017853A1 (de) | 2000-07-12 |
| WO1999015702A1 (en) | 1999-04-01 |
| US6399334B1 (en) | 2002-06-04 |
| US20060269940A1 (en) | 2006-11-30 |
| ES2281138T3 (es) | 2007-09-16 |
| JP2001517460A (ja) | 2001-10-09 |
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Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US |