DE69837453D1 - Ein in vitro Sortierverfahren - Google Patents

Ein in vitro Sortierverfahren

Info

Publication number
DE69837453D1
DE69837453D1 DE69837453T DE69837453T DE69837453D1 DE 69837453 D1 DE69837453 D1 DE 69837453D1 DE 69837453 T DE69837453 T DE 69837453T DE 69837453 T DE69837453 T DE 69837453T DE 69837453 D1 DE69837453 D1 DE 69837453D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid molecule
microcapsules
sorting procedure
vitro sorting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69837453T
Other languages
English (en)
Other versions
DE69837453T2 (de
Inventor
Andrew Griffiths
Dan Tawfik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26311843&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69837453(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9714300.2A external-priority patent/GB9714300D0/en
Priority claimed from GBGB9806393.6A external-priority patent/GB9806393D0/en
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of DE69837453D1 publication Critical patent/DE69837453D1/de
Application granted granted Critical
Publication of DE69837453T2 publication Critical patent/DE69837453T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1062Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
DE69837453T 1997-07-07 1998-06-29 Ein in vitro Sortierverfahren Expired - Lifetime DE69837453T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9714300 1997-07-07
GBGB9714300.2A GB9714300D0 (en) 1997-07-07 1997-07-07 In vitro sorting method
GB9806393 1998-03-25
GBGB9806393.6A GB9806393D0 (en) 1998-03-25 1998-03-25 In vitro sorting method

Publications (2)

Publication Number Publication Date
DE69837453D1 true DE69837453D1 (de) 2007-05-10
DE69837453T2 DE69837453T2 (de) 2007-12-20

Family

ID=26311843

Family Applications (4)

Application Number Title Priority Date Filing Date
DE69841997T Expired - Lifetime DE69841997D1 (de) 1997-07-07 1998-06-29 In-vitro-Sortierverfahren
DE69837453T Expired - Lifetime DE69837453T2 (de) 1997-07-07 1998-06-29 Ein in vitro Sortierverfahren
DE69826697T Expired - Lifetime DE69826697T2 (de) 1997-07-07 1998-06-29 In vitro selektionsmethode
DE69838521T Expired - Lifetime DE69838521T2 (de) 1997-07-07 1998-06-29 Methode zur Erhöhung der Konzentration von Nucleinsäuremolekülen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69841997T Expired - Lifetime DE69841997D1 (de) 1997-07-07 1998-06-29 In-vitro-Sortierverfahren

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE69826697T Expired - Lifetime DE69826697T2 (de) 1997-07-07 1998-06-29 In vitro selektionsmethode
DE69838521T Expired - Lifetime DE69838521T2 (de) 1997-07-07 1998-06-29 Methode zur Erhöhung der Konzentration von Nucleinsäuremolekülen

Country Status (13)

Country Link
US (7) US6489103B1 (de)
EP (6) EP1801214B1 (de)
JP (3) JP4298912B2 (de)
AT (4) ATE487790T1 (de)
AU (1) AU736836B2 (de)
CA (3) CA2295324C (de)
CY (1) CY1111537T1 (de)
DE (4) DE69841997D1 (de)
DK (3) DK1496120T3 (de)
ES (4) ES2294427T3 (de)
GB (1) GB2342094B (de)
PT (2) PT1496120E (de)
WO (1) WO1999002671A1 (de)

Families Citing this family (349)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
ATE487790T1 (de) 1997-07-07 2010-11-15 Medical Res Council In-vitro-sortierverfahren
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
AU2004242526B2 (en) * 1999-01-07 2007-08-30 United Kingdom Research And Innovation Optical Sorting Method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
AU783294B2 (en) * 1999-03-24 2005-10-13 Board Of Regents, The University Of Texas System Linear and circular expression elements
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20050118580A1 (en) * 2001-03-16 2005-06-02 Helmut Merk Nucleic acid which is stabilized against decomposition
JP4838957B2 (ja) * 2001-05-02 2011-12-14 独立行政法人理化学研究所 無細胞タンパク質合成系を用いたタンパク質の製造方法
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0115841D0 (en) 2001-06-28 2001-08-22 Medical Res Council Ligand
WO2003037302A1 (en) * 2001-10-30 2003-05-08 Windsor J Brian Method and system for the co-isolation of cognate dna, rna and protein sequences and method for screening co-isolates for defined activities
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP4359707B2 (ja) 2002-05-22 2009-11-04 独立行政法人理化学研究所 チオレドキシン融合タンパク質発現ベクターを用いた無細胞タンパク質合成系によるタンパク質の製造方法
AUPS298102A0 (en) * 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
JP2006507921A (ja) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20060002935A1 (en) 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
DE60335000D1 (de) 2002-09-06 2010-12-30 Isogenica Ltd In vitro peptid-expressionsbank
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 S12リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
GB0228819D0 (en) * 2002-12-11 2003-01-15 British American Tobacco Co Improvements relating to smoking articles
GB0230203D0 (en) 2002-12-27 2003-02-05 Domantis Ltd Fc fusion
WO2005003375A2 (en) * 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
FI20030615A0 (fi) * 2003-04-24 2003-04-24 Marko Virta Nanopartikkeli bioaffiniteettimäärityksiin
EP2266687A3 (de) 2003-04-10 2011-06-29 The President and Fellows of Harvard College Erzeugung und Kontrolle von Fluiden
WO2004101790A1 (en) 2003-05-14 2004-11-25 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
EP1641809B2 (de) 2003-07-05 2018-10-03 The Johns Hopkins University Verfahren und zusammensetzungen zum nachweis und zur auflistung genetischer variationen
EP2662135A3 (de) 2003-08-27 2013-12-25 President and Fellows of Harvard College Verfahren zur Mischung von Tröpfchen in einem Mikrokanal
ATE386113T1 (de) * 2003-10-01 2008-03-15 Eth Zuerich Verfahren zur in vitro evolution von polypeptiden
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US7927797B2 (en) 2004-01-28 2011-04-19 454 Life Sciences Corporation Nucleic acid amplification with continuous flow emulsion
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
GB0418651D0 (en) * 2004-08-20 2004-09-22 Medical Res Council Method
GB0419628D0 (en) 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
CN101724071A (zh) 2004-10-08 2010-06-09 杜门蒂斯有限公司 抗肿瘤坏死因子受体1的单域抗体及其使用方法
US20080004436A1 (en) * 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型dnaを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
EP2233583A1 (de) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
AU2006220816A1 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
DK1885880T3 (da) * 2005-04-29 2010-11-08 Synthetic Genomics Inc Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikation
EP1910537A1 (de) * 2005-06-06 2008-04-16 454 Life Sciences Corporation Sequenzierung mit gepaarten enden
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
EP2620510B2 (de) 2005-06-15 2020-02-19 Complete Genomics Inc. Einzelne Molekülarrays für genetische und chemische Analyse
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
JP5033949B2 (ja) * 2005-08-12 2012-09-26 国立大学法人豊橋技術科学大学 電気乳化法
WO2007024798A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Apparatus, system, and method using immiscible-fluid-discrete-volumes
EP2546360A1 (de) 2005-10-07 2013-01-16 Callida Genomics, Inc. Selbstangeordnete einzelne Molekülarrays und Verwendungen davon
KR100828714B1 (ko) 2005-10-25 2008-05-09 주식회사 엘지화학 리보핵산을 이용한 멀티플렉스 증폭방법
EP1801212A1 (de) * 2005-12-21 2007-06-27 Eidgenössische Technische Hochschule Zürich Selektion von Biokatalysatoren für die chemische Synthese
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
CA2640024A1 (en) * 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
CN101495654A (zh) * 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
EP2530168B1 (de) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007140015A2 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008103900A2 (en) 2007-02-23 2008-08-28 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CN101680027A (zh) * 2007-03-16 2010-03-24 454生命科学公司 检测hiv耐药变异体的系统和方法
US7776927B2 (en) * 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
JP2010528608A (ja) * 2007-06-01 2010-08-26 454 ライフ サイエンシーズ コーポレイション 複合的な混合物から個々の試料を特定するためのシステムおよび方法
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
CA2688434A1 (en) 2007-06-06 2008-12-11 Domantis Limited Polypeptides, antibody variable domains and antagonists
JP2010531664A (ja) * 2007-06-28 2010-09-30 454 ライフ サイエンシーズ コーポレイション 核酸配列決定における適応試薬制御のためのシステムおよび方法
EP2212434A1 (de) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase-ligation-sequenzierung
DK2213747T3 (da) 2007-11-05 2021-08-09 Riken Fremgangsmåde til fremstilling af membranprotein
EP2215250B1 (de) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzyme zur amplifikation und zum kopieren bisulphit-modifizierter nukleinsäuren
BRPI0819693A2 (pt) 2007-11-30 2020-08-18 Glaxo Group Limited Construção de ligação de antígeno, método para tratar um paciente que sofre de câncer ou uma doença inflamatória, sequência de polinucleotídeo, polinucleotídeo, célula hospedeira transformada ou transfectada recombinante, método para produziruma construção de ligação de antígeno, e, composição farmacêutica
EP2235210B1 (de) 2007-12-21 2015-03-25 President and Fellows of Harvard College Verfahren zur nukleinsäuresequenzierung
EP2489744B1 (de) 2008-02-04 2014-09-03 Life Technologies Corporation Zusammensetzung und Verfahren zum Sequenzieren von Nukleinsäure
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
BRPI0909397A2 (pt) 2008-03-31 2015-12-15 Glaxo Group Ltd composição, uso de uma composição, formulação, dispositivo de liberação, ácido nucléico, vetor, célula hospedeira, e, métodos para produzir um polipeptídeo de fusão
CA2719211C (en) * 2008-04-04 2017-01-24 Allopartis Biotechnologies, Inc. Method of enhancing enzyme activity
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US20100261189A1 (en) 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
EP2358913B1 (de) 2008-11-03 2017-03-22 The Regents of The University of California Nachweisverfahren für modifikationsresistente nukleinsäuren
EP2719774B8 (de) 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Verfahren zur Überwachung von Zuständen durch Sequenzanalyse
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB0822260D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
GB0822261D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
US8685895B2 (en) 2008-12-05 2014-04-01 Carolyn Enever Methods for selecting protease resistant polypeptides
EP3587594B1 (de) 2008-12-19 2022-04-13 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
US8236532B2 (en) * 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
DK2387627T3 (en) 2009-01-15 2016-07-04 Adaptive Biotechnologies Corp Adaptive immunity profiling and methods for producing monoclonal antibodies
US8581209B2 (en) * 2009-01-29 2013-11-12 Southwest Research Institute Fluorescent monitoring of microcapsule oxidation
MX2011008799A (es) 2009-02-19 2011-09-27 Glaxo Group Ltd Polipeptidos, dominios variables de anticuerpos, y antagonistas anti-tnfr1 mejorados.
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
CA2753332A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
US9056299B2 (en) 2009-03-13 2015-06-16 President And Fellows Of Harvard College Scale-up of flow-focusing microfluidic devices
EP2411148B1 (de) 2009-03-23 2018-02-21 Raindance Technologies, Inc. Manipulation von mikrofluidiktröpfchen
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
EP2411054A2 (de) 2009-03-27 2012-02-01 Glaxo Group Limited Arzneifusionen und konjugate
US20110123985A1 (en) * 2009-04-08 2011-05-26 Applied Biosystems, Llc Column enrichment of pcr beads comprising tethered amplicons
WO2010129541A1 (en) * 2009-05-04 2010-11-11 Affomix Corporation Emulsion selection of antibodies
JP2012531202A (ja) 2009-06-25 2012-12-10 フレッド ハチンソン キャンサー リサーチ センター 適応免疫を測定する方法
EP2453920A2 (de) 2009-07-16 2012-05-23 Glaxo Group Limited Antagonisten, verwendung und verfahren zur teilweisen tnfr1-hemmung
JP5099455B2 (ja) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 エマルジョンを用いたrnaの選択的増幅法
CN102574078B (zh) 2009-09-02 2016-05-18 哈佛学院院长等 使用喷射和其它技术产生的多重乳液
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
JP2013506628A (ja) 2009-09-30 2013-02-28 グラクソ グループ リミテッド 延長された半減期を有する薬物融合物及びコンジュゲート
EP2486409A1 (de) 2009-10-09 2012-08-15 Universite De Strasbourg Markiertes nanomaterial auf siliziumbasis mit verbesserten eigenschaften und seine verwendung
US8609339B2 (en) * 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
EA022898B1 (ru) 2009-10-27 2016-03-31 Глаксо Груп Лимитед Стабильные полипептиды, вариабельные домены антитела и антагонисты против tnfr1
CN102648053B (zh) 2009-10-27 2016-04-27 哈佛学院院长等 液滴生成技术
WO2011053845A2 (en) 2009-10-30 2011-05-05 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
EP2333102A1 (de) * 2009-12-14 2011-06-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Identifizierung von RNA-Liganden
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
JP2013516967A (ja) 2010-01-14 2013-05-16 グラクソ グループ リミテッド 肝臓標的化ドメイン抗体
EP3151052A1 (de) 2010-02-01 2017-04-05 Illumina, Inc. Fokussierungsverfahren und optische systeme sowie anordnungen damit
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3392349A1 (de) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digitale analyse von analyten
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
WO2011112465A1 (en) 2010-03-06 2011-09-15 Illumina, Inc. Systems, methods, and apparatuses for detecting optical signals from a sample
KR20130016284A (ko) * 2010-03-17 2013-02-14 바스프 에스이 용융 유화
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
WO2011135041A1 (en) 2010-04-30 2011-11-03 Roche Diagnostics Gmbh System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US8530158B2 (en) 2010-05-10 2013-09-10 Life Technologies Corporation System and method for processing a biological sample
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
ES2669870T3 (es) 2010-07-22 2018-05-29 Gencell Biosystems Limited Células de líquido compuesto
IN2013MN00522A (de) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
CN106399472A (zh) 2010-10-28 2017-02-15 生命技术公司 化学增强型引物组合物、方法和试剂盒
CN103429331B (zh) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
WO2012069557A1 (en) 2010-11-24 2012-05-31 Glaxo Group Limited Multispecific antigen binding proteins targeting hgf
WO2012073045A2 (en) 2010-12-03 2012-06-07 Cyclogenix Ltd Polypeptide scaffold
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP3412778A1 (de) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
EP3736281A1 (de) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
EP2686449B1 (de) 2011-03-18 2020-11-18 Bio-Rad Laboratories, Inc. Multiplexierte digitalassays mit kombinatorischer nutzung von signalen
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
WO2012136790A1 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Compositions comprising fusion proteins or conjugates with an improved half -life
WO2012136792A2 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Cck compositions
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
US9238206B2 (en) 2011-05-23 2016-01-19 President And Fellows Of Harvard College Control of emulsions, including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
EP3461807B1 (de) 2011-06-08 2023-07-12 Life Technologies Corporation Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
US20140220350A1 (en) 2011-07-06 2014-08-07 President And Fellows Of Harvard College Multiple emulsions and techniques for the formation of multiple emulsions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP5924888B2 (ja) * 2011-08-26 2016-05-25 関東化學株式会社 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬
KR20130023091A (ko) * 2011-08-26 2013-03-07 (주)바이오니아 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
EP2768982A4 (de) 2011-10-21 2015-06-03 Adaptive Biotechnologies Corp Quantifizierung von adaptiven immunzellgenomen in einer komplexen mischung von zellen
DE102011085473A1 (de) 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US9824179B2 (en) 2011-12-09 2017-11-21 Adaptive Biotechnologies Corp. Diagnosis of lymphoid malignancies and minimal residual disease detection
EP2788499B1 (de) 2011-12-09 2016-01-13 Illumina, Inc. Expandierte wurzel für polymer-tags
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013101896A1 (en) 2011-12-30 2013-07-04 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
EP2807255B1 (de) 2012-01-25 2017-08-02 Gencell Biosystems Limited Biomolekülisolierung
WO2013116698A2 (en) 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
WO2013134162A2 (en) 2012-03-05 2013-09-12 Sequenta, Inc. Determining paired immune receptor chains from frequency matched subunits
NO2694769T3 (de) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
BR112014027309A2 (pt) 2012-05-08 2017-07-11 Adaptive Biotechnologies Corp composições e métodos para medir e calibrar a tendên-cia de amplificação em reações de pcr multiplexadas
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
US20150167084A1 (en) 2012-07-03 2015-06-18 Sloan Kettering Institute For Cancer Research Quantitative Assessment of Human T-Cell Repertoire Recovery After Allogeneic Hematopoietic Stem Cell Transplantation
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
EP2882872B1 (de) 2012-08-13 2021-10-06 The Regents of The University of California Verfahren und systeme zur erkennung biologischer komponenten
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN113528634A (zh) 2012-08-14 2021-10-22 10X基因组学有限公司 微胶囊组合物及方法
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
AU2013327423B2 (en) 2012-10-01 2017-06-22 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CN104812492A (zh) 2012-11-27 2015-07-29 基因细胞生物系统有限公司 处理液体样品
EP3567116A1 (de) 2012-12-14 2019-11-13 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900543C (en) 2013-02-08 2023-01-31 10X Genomics, Inc. Partitioning and processing of analytes and other species
WO2014144713A2 (en) 2013-03-15 2014-09-18 Immumetrix, Inc. Methods of sequencing the immune repertoire
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US9348897B2 (en) 2013-08-07 2016-05-24 Google Inc. Method and system for providing scheduling suggestions
EP2840148B1 (de) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Verfahren für nukleinsäureamplifikation
EP2848698A1 (de) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System und Methode zur automatisierten Nukleinsäure-Amplifikation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3539944A1 (de) 2013-10-25 2019-09-18 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
US10088973B2 (en) * 2013-11-08 2018-10-02 Google Llc Event scheduling presentation in a graphical user interface environment
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3090063B1 (de) 2013-12-31 2019-11-06 Bio-Rad Laboratories, Inc. Verfahren zur detektion von latentem retrovirus
CN106029885A (zh) 2014-02-10 2016-10-12 基因细胞生物系统有限公司 复合液体池(clc)介导的核酸文库制备装置及其使用方法
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
JP2017526046A (ja) 2014-06-26 2017-09-07 10エックス ゲノミクス,インコーポレイテッド 核酸配列アセンブルのプロセス及びシステム
EP3161160B1 (de) 2014-06-26 2021-10-13 10X Genomics, Inc. Verfahren zur analyse von nukleinsäuren aus einzelzellen oder zellpopulationen
WO2015200717A2 (en) 2014-06-27 2015-12-30 The Regents Of The University Of California Pcr-activated sorting (pas)
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
WO2016053883A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
EP3209419A4 (de) 2014-10-22 2018-10-03 The Regents of The University of California Hochauflösender mikrotröpfchendrucker
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CA2968417A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
EP3256606B1 (de) 2015-02-09 2019-05-22 10X Genomics, Inc. Systeme und verfahren zur bestimmung struktureller varianten
JP2018505688A (ja) 2015-02-24 2018-03-01 10エックス ゲノミクス,インコーポレイテッド 標的化核酸配列包括度(coverage)のための方法
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
CA2976580A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
WO2016150771A1 (en) 2015-03-20 2016-09-29 Novozymes A/S Droplet-based selection by injection
EP4089398A1 (de) 2015-03-24 2022-11-16 Illumina, Inc. Trägervorrichtungen und systeme zum abbilden von proben zur biologischen oder chemischen analyse
WO2016161273A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
CN107864658A (zh) * 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
WO2016164583A2 (en) 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having enhanced activity
EP3307908B1 (de) 2015-06-09 2019-09-11 Life Technologies Corporation Verfahren zur molekularen markierung
US11618777B2 (en) 2015-07-31 2023-04-04 Shigeyuki Yokoyama Method of manufacturing membrane protein and utilization thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CN108289797B (zh) 2015-10-13 2022-01-28 哈佛学院院长及董事 用于制备和使用凝胶微球的系统和方法
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US10774370B2 (en) 2015-12-04 2020-09-15 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
EP3414341A4 (de) 2016-02-11 2019-10-09 10X Genomics, Inc. Systeme, verfahren und medien zur de-novo-anordnung von ganzen genomsequenzdaten
CN109312333A (zh) 2016-04-07 2019-02-05 诺维信公司 选择具有蛋白酶活性的酶的方法
CN109072480A (zh) * 2016-04-29 2018-12-21 生物辐射实验室股份有限公司 数字聚合酶保真度测定
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018031691A1 (en) 2016-08-10 2018-02-15 The Regents Of The University Of California Combined multiple-displacement amplification and pcr in an emulsion microdroplet
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
MY194951A (en) 2016-10-14 2022-12-28 Illumina Inc Cartridge assembly
WO2018118968A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Emulsion-based screening methods
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183A3 (de) 2017-01-30 2024-02-21 10X Genomics, Inc. Verfahren und systeme zur tröpfchenbasierten einzelzellenbarcodierung
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
GB201707143D0 (en) 2017-05-04 2017-06-21 Plasticell Ltd Method for producing cells
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076955A1 (en) 2017-10-18 2019-04-25 Novozymes A/S IMMUNOLOGICAL ASSAY FOR THE STABILITY OF A BIO-ACTIVE POLYPEPTIDE
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
EP3697531B1 (de) 2017-10-20 2022-07-27 Stilla Technologies Emulsionen mit verbesserter stabilität
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (de) 2017-10-27 2022-12-28 10X Genomics, Inc. Verfahren zur probenvorbereitung und -analyse
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
EP3752832A1 (de) 2018-02-12 2020-12-23 10X Genomics, Inc. Verfahren zur charakterisierung mehrerer analyten aus einzelnen zellen oder zellpopulationen
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3973074A4 (de) 2019-05-22 2023-09-06 Mission Bio, Inc. Verfahren und vorrichtung zur simultanen gezielten sequenzierung von dna, rna und protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN116997411A (zh) 2021-01-13 2023-11-03 加利福尼亚太平洋生物科学股份有限公司 使用胶体组装的表面结构化
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023225488A1 (en) 2022-05-16 2023-11-23 Enkefalos Biosciences, Inc. Blood-brain barrier translocating peptides and related molecules and methods of use thereof
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2747570A (en) * 1954-05-19 1956-05-29 Jobst Conrad Means for assisting return circulation of fluids in an animal body
US3826249A (en) * 1973-01-30 1974-07-30 A Lee Leg constricting apparatus
US3908642A (en) * 1973-10-29 1975-09-30 Pred Vinmont Means for aerating and applying air pulsations within casts
US4206751A (en) * 1978-03-31 1980-06-10 Minnesota Mining And Manufacturing Company Intermittent compression device
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US5099030A (en) * 1987-03-09 1992-03-24 The Procter & Gamble Company Novel compounds, pharmaceutical compositions, and methods for treating inflammation and pain
EP0494955B1 (de) * 1989-10-05 1998-07-15 Optein, Inc. Zellfreie synthese und isolierung von genen und polypeptiden
EP0515506B1 (de) * 1990-02-16 2000-01-05 F. Hoffmann-La Roche Ag Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
DE476178T1 (de) 1990-09-21 1992-07-23 Bioplex Medical B.V., Vaals, Nl Geraet zum anbringen von blutstillendem stoff auf perforierten blutgefaessen.
US5955341A (en) * 1991-04-10 1999-09-21 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
GB9107628D0 (en) * 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
CA2114950A1 (en) 1991-08-10 1993-02-11 Michael J. Embleton Treatment of cell populations
AU2872092A (en) * 1991-10-15 1993-05-21 Multilyte Limited Binding assay employing labelled reagent
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
DE4223169C1 (de) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
IL104384A (en) * 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
WO1994024314A1 (en) * 1993-04-19 1994-10-27 Kauffman Stuart A Random chemistry for the generation of new compounds
AU6707194A (en) 1993-04-19 1994-11-08 Medisorb Technologies International L.P. Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression
WO1995011922A1 (en) * 1993-10-29 1995-05-04 Affymax Technologies N.V. In vitro peptide and antibody display libraries
DE4402038A1 (de) 1994-01-25 1995-07-27 Borries Horst Von Durchdrückpackung
EP0804249A2 (de) 1994-03-15 1997-11-05 Brown University Research Foundation Polymeres system zur freisetzung von genen
US5508348A (en) * 1994-08-12 1996-04-16 The Research Foundation Of Suny Inverted emulsion pathways to conductive polymers
US5680283A (en) * 1994-09-30 1997-10-21 Kabushiki Kaisha Toshiba Magnetic head and magnetic disk drive
HUP9801871A3 (en) 1995-04-24 1999-12-28 Chromaxome Corp San Diego Methods for generating and screening novel metabolic pathways
US5756122A (en) 1995-06-07 1998-05-26 Georgetown University Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells
US5910408A (en) * 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US5989892A (en) 1995-06-14 1999-11-23 Tonen Corporation Microorganisms, demulsifiers and processes for breaking an emulsion
AU6691496A (en) 1995-08-01 1997-02-26 Advanced Therapies, Inc. Enhanced artificial viral envelopes for cellular delivery of therapeutic substances
DE19646372C1 (de) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5876711A (en) * 1996-05-31 1999-03-02 Onyx Pharmaceuticals, Inc. Methods and compositions for determining the tumor suppressor status of cells
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US5876771A (en) * 1996-06-20 1999-03-02 Tetra Laval Holdings & Finance, Sa Process and article for determining the residence time of a food particle
WO1998013502A2 (en) 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
DE69835143T2 (de) 1997-01-21 2007-06-06 The General Hospital Corp., Boston Selektion von proteinen mittels rns-protein fusionen
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB9703369D0 (en) * 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
WO1998041869A1 (en) 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
US6048551A (en) * 1997-03-27 2000-04-11 Hilfinger; John M. Microsphere encapsulation of gene transfer vectors
ATE487790T1 (de) * 1997-07-07 2010-11-15 Medical Res Council In-vitro-sortierverfahren
JP2981547B1 (ja) 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
EP1100889A4 (de) 1998-07-17 2002-03-06 Mirus Corp Micell systeme
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
CN1189159C (zh) 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US6936264B2 (en) 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
JP3570714B2 (ja) * 2001-05-24 2004-09-29 株式会社リコー 現像剤収納容器及び画像形成装置
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
WO2004083443A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids

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