EP0271155B2 - Enzymatic dishwashing and rinsing process - Google Patents

Enzymatic dishwashing and rinsing process Download PDF

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Publication number
EP0271155B2
EP0271155B2 EP87202387A EP87202387A EP0271155B2 EP 0271155 B2 EP0271155 B2 EP 0271155B2 EP 87202387 A EP87202387 A EP 87202387A EP 87202387 A EP87202387 A EP 87202387A EP 0271155 B2 EP0271155 B2 EP 0271155B2
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EP
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Prior art keywords
weight
lipase
pseudomonas
use according
dishwashing
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EP87202387A
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German (de)
French (fr)
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EP0271155A2 (en
EP0271155B1 (en
EP0271155A3 (en
Inventor
Willem Robert Van Dijk
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Unilever PLC
Unilever NV
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Unilever PLC
Unilever NV
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the present invention relates to enzymatic dishwashing or rinsing processes using compositions comprising lipolytic enzymes.
  • EP-A-0 130 064 (Novo) concerns detergent additives based on Fusarium oxysporum lipase (e.g. 20,000-100,000 LU/g, used in detergent at e.g. 0.2-2% w/w.
  • Fusarium oxysporum lipase e.g. 20,000-100,000 LU/g, used in detergent at e.g. 0.2-2% w/w.
  • EP-A-0 205 208 discloses enzymatic detergent compositions comprising lipase with 1-40% anionic surfactant and builders.
  • the lipases disclosed are those that cross-react with antibody against lipase from Pseudomonas fluorescens 1AM 1057.
  • EP 0 206 930 (Unilever) (not prepublished) also discloses enzymatic detergent compositions comprising similar lipases together with combinations of anionic and nonionic detergent.
  • dishwashing compositions have been proposed in the art.
  • enzymes mainly amylases and/or proteases have been proposed for inclusion in dishwashing compositions.
  • Lipases have also been suggested, but have received far less attention that the amylases and/or proteases.
  • Dishwashing compositions in particular machine dishwashing compositions which are used in the main wash step of a machine dishwashing operation, have in general a satisfactory cleaning performance. However, frequently the article cleaned with such products still do not have a satisfactory visual appearance after rinsing and drying, showing film or spots.
  • main wash liquor is usually carried over from the main wash step to the rinse step, causing some deposition of soil resulting in insoluble calcium salts on the articles to be rinsed, which results in visible film or spots on the articles when they are dry. In particular with glass articles, this causes an unsightly visual appearance.
  • the invention provides the use of a lipolytic enzyme as a spot reducing agent a process for machine dishwashing or rinsing, comprising treating dishes with an aqueous liquid having dissolved or dispersed therein an effective amount of an enzymatic dishwashing or rinsing composition comprising from 0.5-10% by weight of a detergent surfactant and from 0-90% by weight of a builder and from 0.005-100 LU/mg of a lipolytic enzyme.
  • the lipases used according to the present invention, may be of any suitable origin such as yeasts, fungi and bacteria. Preferably, however, they are of bacterial origin, and particularly preferably they belong to the class of bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var lipolyticum NRRL B-3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilisation and Development Division at Peoria, Illinois, under the number NRRL B-3673.
  • This lipase will hereinafter be referred to as "Toyo Jozo" lipase.
  • the preferred lipases to be used in the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133 , pages 76-79 (1950)).
  • the preparation of the antiserum is carried out as follows: Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme:
  • the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 2 5 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • All lipases showing a positive immunological cross reaction with the Toyo Jozo lipase antibody as hereabove described are preferred lipases for use in the present invention.
  • Typical examples thereof are the lipases ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp.
  • Chromobacter viscosum e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli .
  • the lipases used in the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
  • lipases can be used in their non-purified form, or in a purified form, e.g. purified with the aid of well-known adsorption methods, such as a phenylsepharose-adsorption techniques.
  • the composition used according to the invention may furthermore comprise the usual ingredients of dishwashing or rinse compositions.
  • it may contain one or more alkali salts commonly used in dishwashing compositions.
  • it may contain organic and/or inorganic builders such as the alkali metal ortho-, pyro and tripolyphosphates and hexametaphosphates, silicates, carbonates, zeolites, borates, citrates, carboxymethyloxy-succinates, nitrilotriacetates and ethylenediamine-tetraacetates, polymeric polyelectrolytes such as polyacrylates, polymaleates, and other known organic and inorganic builder compounds.
  • the amount of builders in the composition varies from 10-90% by weight, generally from 30-70% by weight.
  • the composition may also contain a detergent-active compound. If a detergent-active compound is included, it usually is in an amount of from 0.5-10%, usually 1-5%. Any well-known type of detergent active compound may be used, such as soaps, synthetic anionic, non-ionic, amphoteric detergent surfactant and mixtures thereof. Preferably, a nonionic detergent surfactant is used, especially a low-foaming one. Suitable examples of such nonionic detergent surfactants can easily be found in M.Schick "Nonionic Surfactants" (1967).
  • composition may furthermore contain other useful additives such as bleaching agents, bleaching agent activators, hydrotropes, fillers, perfumes, colouring agents, germicides, soil-suspending agents, aminopolyphosphonic acids and alkali metal or alkaline earth metal salts thereof, clays such as hectorites, anti-corrosion agents such as fatty acids, benztriazole and so on.
  • Other enzymes such as proteases, amylases, oxidases may also be included.
  • the peroxygen type bleaching agents preferably with a bleach precursor such as TAED are suitable for inclusion in the machine dishwashing compositions.
  • a typical example of a conventional machine dishwashing composition usually contains an alkali metal tripolyphosphate in an amount of from 20-60%, an alkali metal silicate in an amount of from 40-80%, or an alkali metal disilicate in an amount of 5-30% by weight a peroxy type bleaching agent in an amount of from 1-15%, a low-foaming detergent surfactant in an amount of from 0.5-5%, and minor ingredients such as perfumes, colouring agents, hydrotropes, fillers, etc.
  • a rinse composition When formulated as a rinse composition, it may contain from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc., the balance being water.
  • a nonionic surfactant from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc.
  • the balance being water.
  • the products of the invention can be formulated in any desirable form, such as powders, granulates, cakes, bars, pastes, liquids etc. The invention will further be illustrated by way of example.
  • detergent composition dosage 30 g lipase dosage 2.5 g ( 15 LU/ml) water hardness main wash 27° GH flood 9° GH rinse 1 9° GH rinse 2 9° GH final rinse 9° GH wash temperature and time 55°C - 60 minutes articles to be cleaned clean dishes clean glasses test soil 35 g egg-yolk, added at the beginning of the main wash.
  • the detergent composition had the following formulation: in gr. sodium tripolyphosphate 11.61 sodium carbonate 2.7 TAED (59.7%) 1.81 sodium perborate 2.43 calcium-Dequest (47.6%) 0.397 sodium disilicate 3.3 low foaming nonionic (Plurafac RA40) 0.405 hectorite clay (Laponite XLS) 0.675 sodium sulphate 5.610 amylase (4.8 Maltose units/mg) 0.540
  • the Toyo Jozo lipase was used as lipase.
  • the test was carried out in two runs, one without and one with lipase.
  • the glasses were appraised by an expert panel as to their visual appearance, and were given a score according to the following scale: Film Spots 1. no film 1. no spots 2. slight film 2. 1-5 spots 3. clear film 3. 5-10 spots 4. heavy film 4. 11-20 spots 5. more than 20 spots
  • Example 1 The test of Example 1 was repeated, but under the following different conditions: wash temperature 65°C soil standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place. run 1 no lipase added in the final rinse run 2 2.5 g Toyo Jozo lipase was added to the final rinse (15 LU/ml).
  • the main wash composition had the following formulation: % by weight sodium tripolyphosphate 36 sodium carbonate 9 sodium disilicate 15 TAED 3.8 sodium perborate tetrahydrate 8.8 potassium metaborate 0.35 sodium metaborate 0.85 calcium-Dequest 0.63 Amylase (4.8 MU/mg) 1.8 Esperase (a protease; 675 GU/mg) 1.8 low-foaming nonionic (Plurafac RA 40) 1.35 hectorite (Laponite XLS) 2.25 benzotriazole 0.1 sodium sulphate 14.0 water 9.5
  • Example 2 Replacing the Toyo Jozo lipase by the Amano-P lipase or Lipase MY (ex Candida cylindracea ) or Amano AP-6 lipase (ex Aspergillus niger ) in Example 2 gave similar results.
  • the following formulation is an example of a rinse aid composition to which lipases can be added: % by weight low-foaming nonionic (Plurafac RA 30) 10 citric acid 18 sodium xylene sulphonate 3.0 dye 0.003 butylated hydroxytoluene 0.1 water q.s.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)

Description

  • The present invention relates to enzymatic dishwashing or rinsing processes using compositions comprising lipolytic enzymes.
  • EP-A-0 130 064 (Novo) concerns detergent additives based on Fusarium oxysporum lipase (e.g. 20,000-100,000 LU/g, used in detergent at e.g. 0.2-2% w/w.
  • DE-A-1 930 636 (= GB 1 273 545) (Albright & Wilson) concerns cleaning compositions based on protease, lipase and/or decarboxylase (of unspecified type) and surfactant, used inter alia for dishwashing.
  • EP-A-0 205 208 (Unilever) (not prepublished) discloses enzymatic detergent compositions comprising lipase with 1-40% anionic surfactant and builders. The lipases disclosed are those that cross-react with antibody against lipase from Pseudomonas fluorescens 1AM 1057.
  • EP 0 206 930 (Unilever) (not prepublished) also discloses enzymatic detergent compositions comprising similar lipases together with combinations of anionic and nonionic detergent.
  • Thus, enzymatic dishwashing compositions have been proposed in the art. As enzymes, mainly amylases and/or proteases have been proposed for inclusion in dishwashing compositions. Lipases have also been suggested, but have received far less attention that the amylases and/or proteases. Dishwashing compositions, in particular machine dishwashing compositions which are used in the main wash step of a machine dishwashing operation, have in general a satisfactory cleaning performance. However, frequently the article cleaned with such products still do not have a satisfactory visual appearance after rinsing and drying, showing film or spots. Some main wash liquor is usually carried over from the main wash step to the rinse step, causing some deposition of soil resulting in insoluble calcium salts on the articles to be rinsed, which results in visible film or spots on the articles when they are dry. In particular with glass articles, this causes an unsightly visual appearance.
  • We have now found that the addition of lipases to a main wash dishwashing composition or to a rinse composition significantly reduced the formation of film or spots on the articles cleaned or rinsed with such a composition.
  • Accordingly the invention provides the use of a lipolytic enzyme as a spot reducing agent a process for machine dishwashing or rinsing, comprising treating dishes with an aqueous liquid having dissolved or dispersed therein an effective amount of an enzymatic dishwashing or rinsing composition comprising from 0.5-10% by weight of a detergent surfactant and from 0-90% by weight of a builder and from 0.005-100 LU/mg of a lipolytic enzyme.
  • The lipases, used according to the present invention, may be of any suitable origin such as yeasts, fungi and bacteria. Preferably, however, they are of bacterial origin, and particularly preferably they belong to the class of bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var lipolyticum NRRL B-3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilisation and Development Division at Peoria, Illinois, under the number NRRL B-3673. This lipase will hereinafter be referred to as "Toyo Jozo" lipase. The preferred lipases to be used in the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133, pages 76-79 (1950)).
  • The preparation of the antiserum is carried out as follows:
    Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme:
    • day 0 :
    antigen in complete Freund's adjuvant
    day 4 :
    antigen in complete Freund's adjuvant
    day 32 :
    antigen in incomplete Freund's adjuvant
    day 60 :
    booster of antigen in incomplete Freund's adjuvant.
  • The serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • The titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • All lipases showing a positive immunological cross reaction with the Toyo Jozo lipase antibody as hereabove described are preferred lipases for use in the present invention. Typical examples thereof are the lipases ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp. available under the trade name Amano-CES, the lipase ex Pseudomonas cepacia, lipases ex Chromobacter viscosum, e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli.
  • The lipases used in the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
  • A Lipase Unit (LU) is that amount of lipase which produces 1 µmol of titratable fatty acid per minute in a pH stat. under the following conditions: temperature 30°C; pH = 9.0; substrate is an emulsion of 3.3 wt.% of olive oil and 3.3% gum arabic, in the presence of 13 mmo1/1 Ca2+ and 20 mmo1/1 NaC1 in 5 mmo1/1 Tris-buffer.
  • Naturally, mixtures of the above lipases can be used. The lipases can be used in their non-purified form, or in a purified form, e.g. purified with the aid of well-known adsorption methods, such as a phenylsepharose-adsorption techniques.
  • The composition used according to the invention may furthermore comprise the usual ingredients of dishwashing or rinse compositions. Thus it may contain one or more alkali salts commonly used in dishwashing compositions. Thus, it may contain organic and/or inorganic builders such as the alkali metal ortho-, pyro and tripolyphosphates and hexametaphosphates, silicates, carbonates, zeolites, borates, citrates, carboxymethyloxy-succinates, nitrilotriacetates and ethylenediamine-tetraacetates, polymeric polyelectrolytes such as polyacrylates, polymaleates, and other known organic and inorganic builder compounds.
  • Usually, the amount of builders in the composition varies from 10-90% by weight, generally from 30-70% by weight.
  • The composition may also contain a detergent-active compound. If a detergent-active compound is included, it usually is in an amount of from 0.5-10%, usually 1-5%. Any well-known type of detergent active compound may be used, such as soaps, synthetic anionic, non-ionic, amphoteric detergent surfactant and mixtures thereof. Preferably, a nonionic detergent surfactant is used, especially a low-foaming one. Suitable examples of such nonionic detergent surfactants can easily be found in M.Schick "Nonionic Surfactants" (1967).
  • The composition may furthermore contain other useful additives such as bleaching agents, bleaching agent activators, hydrotropes, fillers, perfumes, colouring agents, germicides, soil-suspending agents, aminopolyphosphonic acids and alkali metal or alkaline earth metal salts thereof, clays such as hectorites, anti-corrosion agents such as fatty acids, benztriazole and so on. Other enzymes such as proteases, amylases, oxidases may also be included.
  • As bleaching agents the peroxygen type bleaching agents, preferably with a bleach precursor such as TAED are suitable for inclusion in the machine dishwashing compositions.
  • A typical example of a conventional machine dishwashing composition usually contains an alkali metal tripolyphosphate in an amount of from 20-60%, an alkali metal silicate in an amount of from 40-80%, or an alkali metal disilicate in an amount of 5-30% by weight a peroxy type bleaching agent in an amount of from 1-15%, a low-foaming detergent surfactant in an amount of from 0.5-5%, and minor ingredients such as perfumes, colouring agents, hydrotropes, fillers, etc.
  • When formulated as a rinse composition, it may contain from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc., the balance being water. The products of the invention can be formulated in any desirable form, such as powders, granulates, cakes, bars, pastes, liquids etc. The invention will further be illustrated by way of example.
    • Example 1
  • In a commercial dishwashing machine tests were carried out under the following conditions:
    detergent composition dosage 30 g
    lipase dosage 2.5 g (= 15 LU/ml)
    water hardness main wash 27° GH
    flood 9° GH
    rinse 1 9° GH
    rinse 2 9° GH
    final rinse 9° GH
    wash temperature and time 55°C - 60 minutes
    articles to be cleaned clean dishes
    clean glasses
    test soil 35 g egg-yolk, added at the beginning of the main wash.
  • The detergent composition had the following formulation:
    in gr.
    sodium tripolyphosphate 11.61
    sodium carbonate 2.7
    TAED (59.7%) 1.81
    sodium perborate 2.43
    calcium-Dequest (47.6%) 0.397
    sodium disilicate 3.3
    low foaming nonionic (Plurafac RA40) 0.405
    hectorite clay (Laponite XLS) 0.675
    sodium sulphate 5.610
    amylase (4.8 Maltose units/mg) 0.540
  • As lipase, the Toyo Jozo lipase was used.
  • The test was carried out in two runs, one without and one with lipase.
  • The glasses were appraised by an expert panel as to their visual appearance, and were given a score according to the following scale:
    Film Spots
    1. no film 1. no spots
    2. slight film 2. 1-5 spots
    3. clear film 3. 5-10 spots
    4. heavy film 4. 11-20 spots
    5. more than 20 spots
  • The following results were obtained:
    run 1 (no lipase) run 2 (with lipases)
    average film score 2.3 2.3
    average number of spots 250 100
    • Example 2
  • The test of Example 1 was repeated, but under the following different conditions:
    wash temperature 65°C
    soil standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
    run 1 no lipase added in the final rinse
    run 2 2.5 g Toyo Jozo lipase was added to the final rinse (15 LU/ml).
  • The main wash composition had the following formulation:
    % by weight
    sodium tripolyphosphate 36
    sodium carbonate 9
    sodium disilicate 15
    TAED 3.8
    sodium perborate tetrahydrate 8.8
    potassium metaborate 0.35
    sodium metaborate 0.85
    calcium-Dequest 0.63
    Amylase (4.8 MU/mg) 1.8
    Esperase (a protease; 675 GU/mg) 1.8
    low-foaming nonionic (Plurafac RA 40) 1.35
    hectorite (Laponite XLS) 2.25
    benzotriazole 0.1
    sodium sulphate 14.0
    water 9.5
  • The following spot and film scores of the glasses were obtained:
    run 1 (no lipase) run 2 (with lipases)
    average film score 1 1
    average spot score 5 2.7
    • Example 3
  • Replacing the Toyo Jozo lipase by the Amano-P lipase or Lipase MY (ex Candida cylindracea) or Amano AP-6 lipase (ex Aspergillus niger) in Example 2 gave similar results.
    • Example 4
  • The following formulation is an example of a rinse aid composition to which lipases can be added:
    % by weight
    low-foaming nonionic (Plurafac RA 30) 10
    citric acid 18
    sodium xylene sulphonate 3.0
    dye 0.003
    butylated hydroxytoluene 0.1
    water q.s.

Claims (5)

  1. Use of a lipolytic enzyme as a spot reducing agent in a process for machine dishwashing or rinsing, comprising treating dishes with an aqueous liquid having dissolved or dispersed therein an effective amount of an enzymatic dishwashing or rinsing composition comprising from 0.5-10% by weight of a detergent surfactant and from 0-90% by weight of a builder and from 0.005-100 LU/mg of a lipolytic enzyme.
  2. Use according to claim 1, characterised in that the lipase is a bacterial lipase which shows a positive immunological cross-reaction with the antibody of the lipase, produced by Chromobacter viscosum var. lipolyticum NRRL B 3673.
  3. Use according to claim 2, characterised in that the lipase is producible by the microorganism selected from the group consisting of Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas nitroreducens var. lipolyticum, Pseudomonas cepacia, Pseudomonas gladioli and Chromobacter viscosum.
  4. Use according to any of claims 1-3 in a machine dishwashing process, characterised in that there is used a composition containing from 20-60% by weight of an alkali metal silicate, from 1-15% by weight of a peroxy type bleaching agent and from 0.5-5% by weight of a low-foaming nonionic detergent.
  5. Use according to any of claims 1-3 in a machine rinsing process, characterised in that there is used a composition containing, in an aqueous medium, from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an organic or inorganic acid and from 1-5% by weight of a hydrotrope.
EP87202387A 1986-12-10 1987-12-02 Enzymatic dishwashing and rinsing process Expired - Lifetime EP0271155B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8629538 1986-12-10
GB868629538A GB8629538D0 (en) 1986-12-10 1986-12-10 Enzymatic dishwashing & rinsing composition

Publications (4)

Publication Number Publication Date
EP0271155A2 EP0271155A2 (en) 1988-06-15
EP0271155A3 EP0271155A3 (en) 1988-08-17
EP0271155B1 EP0271155B1 (en) 1991-09-04
EP0271155B2 true EP0271155B2 (en) 2000-09-06

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EP87202387A Expired - Lifetime EP0271155B2 (en) 1986-12-10 1987-12-02 Enzymatic dishwashing and rinsing process

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EP (1) EP0271155B2 (en)
JP (1) JPS63161086A (en)
AU (1) AU609755B2 (en)
BR (1) BR8706685A (en)
DE (1) DE3772726D1 (en)
ES (1) ES2025635T5 (en)
GB (1) GB8629538D0 (en)
ZA (1) ZA879297B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109563449A (en) * 2016-06-23 2019-04-02 诺维信公司 Purposes, composition and the method for removing dirt of enzyme

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4933287A (en) * 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
BE1001436A3 (en) * 1988-02-22 1989-10-31 Synfina Sa New lipase and detergent compositions containing.
GB8813688D0 (en) * 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing composition
GB8813687D0 (en) * 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing & rinsing composition
JPH0277498A (en) * 1988-09-14 1990-03-16 Lion Corp Detergent composition for use in automatic dishwasher
US4908150A (en) * 1989-02-02 1990-03-13 Lever Brothers Company Stabilized lipolytic enzyme-containing liquid detergent composition
JPH02245129A (en) * 1989-03-17 1990-09-28 Novo Nordisk As Enzymatically removing method for stain on stock
GB8923788D0 (en) * 1989-10-23 1989-12-13 Unilever Plc Enzymatic detergent compositions and their use
JP2620384B2 (en) * 1989-12-20 1997-06-11 花王株式会社 Dishwashing composition for automatic dishwashers
JPH0699715B2 (en) * 1990-07-11 1994-12-07 花王株式会社 Detergent composition for automatic dishwasher
GB2247025A (en) * 1990-08-13 1992-02-19 Unilever Plc Enzymatic dishwashing and rinsing composition
US5527484A (en) * 1991-05-31 1996-06-18 Colgate-Palmolive Co. Phosphate containing powdered automatic dishwashing composition with enzymes
TR28788A (en) 1993-05-25 1997-03-25 Henkel Ecolab Gmbh & Co Ohg Methods and equipment for machine-dishwashing cleaning.
DE4324106C1 (en) * 1993-05-25 1994-09-15 Henkel Ecolab Gmbh & Co Ohg Method and device for the metered addition of active ingredients to a rinsing machine
CN1132525A (en) * 1993-08-10 1996-10-02 普罗格特-甘布尔公司 Composition containing lipase for manually washing tableware
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
EP0846155B1 (en) * 1995-08-25 2000-02-09 Henkel Kommanditgesellschaft auf Aktien Use of lipases in low-alkaline mechanical dishwashing agents
US20080293607A1 (en) 2007-03-09 2008-11-27 Jones Brian E Alkaliphilic Bacillus Species alpha-Amylase Variants, Compositions Comprising alpha-Amylase Variants, And Methods of Use
US9040279B2 (en) 2008-06-06 2015-05-26 Danisco Us Inc. Saccharification enzyme composition and method of saccharification thereof
EP2310521A2 (en) 2008-06-06 2011-04-20 Danisco US Inc. Production of glucose from starch using alpha-amylases from bacillus subtilis
DK2297313T3 (en) 2008-06-06 2015-06-08 Danisco Us Inc ALPHA AMYLASE VARIETIES OF BACILLUS SUBTILIS AND METHODS OF USING IT
CA2738447C (en) 2008-09-25 2019-01-08 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
CA2778471A1 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
JP5246300B2 (en) * 2011-06-14 2013-07-24 Ｊｆｅスチール株式会社 Body side structure

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2071237A5 (en) † 1969-12-22 1971-09-17 Kronwitter Wolfram Dish washers - using amylase as cleaning agent
DE2062465A1 (en) † 1970-12-18 1972-06-22 Henkel & Cie. GmbH, 4000 Düsseldorf Scouring agent - for dish washing machines, contg aminopolycarboxylic acid and alkali silicate
US4101457A (en) † 1975-11-28 1978-07-18 The Procter & Gamble Company Enzyme-containing automatic dishwashing composition
US4162987A (en) † 1976-06-24 1979-07-31 The Procter & Gamble Company Enzyme-containing automatic dishwashing detergent composition
EP0005131A2 (en) † 1978-04-21 1979-10-31 Peter Michael John Bedding Methods and materials for cleaning soft contact lenses
EP0139329A2 (en) † 1983-10-20 1985-05-02 Unilever N.V. Dishwashing compositions
EP0171008A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0171007A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0171006A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0266904A2 (en) † 1986-10-07 1988-05-11 Unilever Plc Machine dish washing composition containing dipicolinic acid

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) * 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
US3950277A (en) * 1973-07-25 1976-04-13 The Procter & Gamble Company Laundry pre-soak compositions
US4421664A (en) * 1982-06-18 1983-12-20 Economics Laboratory, Inc. Compatible enzyme and oxidant bleaches containing cleaning composition
DK289083A (en) * 1983-06-23 1984-12-24 Novo Industri As LIPASE, PROCEDURE FOR PREPARING THEREOF AND ITS APPLICATION
GB8514707D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514708D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (en) * 1985-08-07 1989-04-24 Novo Industri As ENZYMATIC DETERGENT ADDITIVE, DETERGENT AND METHOD FOR WASHING TEXTILES
JPS62129397A (en) * 1985-11-29 1987-06-11 ライオン株式会社 Detergent composition
JPH0689355B2 (en) * 1986-05-06 1994-11-09 サンスタ−株式会社 Cleaning composition
JPS6434559A (en) * 1987-07-30 1989-02-06 Seiko Epson Corp Structure for taking out cavity
JPS6434560A (en) * 1987-07-30 1989-02-06 Seiko Epson Corp Die structure

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2071237A5 (en) † 1969-12-22 1971-09-17 Kronwitter Wolfram Dish washers - using amylase as cleaning agent
DE2062465A1 (en) † 1970-12-18 1972-06-22 Henkel & Cie. GmbH, 4000 Düsseldorf Scouring agent - for dish washing machines, contg aminopolycarboxylic acid and alkali silicate
US4101457A (en) † 1975-11-28 1978-07-18 The Procter & Gamble Company Enzyme-containing automatic dishwashing composition
US4162987A (en) † 1976-06-24 1979-07-31 The Procter & Gamble Company Enzyme-containing automatic dishwashing detergent composition
EP0005131A2 (en) † 1978-04-21 1979-10-31 Peter Michael John Bedding Methods and materials for cleaning soft contact lenses
EP0139329A2 (en) † 1983-10-20 1985-05-02 Unilever N.V. Dishwashing compositions
EP0171008A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0171007A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0171006A2 (en) † 1984-08-04 1986-02-12 Henkel Kommanditgesellschaft auf Aktien Dish cleaning agent
EP0266904A2 (en) † 1986-10-07 1988-05-11 Unilever Plc Machine dish washing composition containing dipicolinic acid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109563449A (en) * 2016-06-23 2019-04-02 诺维信公司 Purposes, composition and the method for removing dirt of enzyme

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AU8222387A (en) 1988-06-16
ES2025635T5 (en) 2001-01-16
ES2025635T3 (en) 1992-04-01
JPS63161086A (en) 1988-07-04
EP0271155A2 (en) 1988-06-15
DE3772726D1 (en) 1991-10-10
EP0271155B1 (en) 1991-09-04
ZA879297B (en) 1989-08-30
GB8629538D0 (en) 1987-01-21
EP0271155A3 (en) 1988-08-17
BR8706685A (en) 1988-07-19

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