EP0792357A4 - Chemical modification of repetitive polymers to enhance water solubility - Google Patents

Chemical modification of repetitive polymers to enhance water solubility

Info

Publication number
EP0792357A4
EP0792357A4 EP95938187A EP95938187A EP0792357A4 EP 0792357 A4 EP0792357 A4 EP 0792357A4 EP 95938187 A EP95938187 A EP 95938187A EP 95938187 A EP95938187 A EP 95938187A EP 0792357 A4 EP0792357 A4 EP 0792357A4
Authority
EP
European Patent Office
Prior art keywords
chemical modification
water solubility
protein
enhance water
repetitive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP95938187A
Other languages
German (de)
French (fr)
Other versions
EP0792357B1 (en
EP0792357A1 (en
Inventor
Erwin R Stedronsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Protein Polymer Technologies Inc
Original Assignee
Protein Polymer Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protein Polymer Technologies Inc filed Critical Protein Polymer Technologies Inc
Publication of EP0792357A1 publication Critical patent/EP0792357A1/en
Publication of EP0792357A4 publication Critical patent/EP0792357A4/en
Application granted granted Critical
Publication of EP0792357B1 publication Critical patent/EP0792357B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans

Abstract

Highly repetitive proteins which are relatively insoluble in water are chemically modified to increase solubility. The protein is reacted with a functionalizing agent to introduce additional polar functionalities and disrupt the order of the protein. The solubility of the protein in water is increased by the chemical modification, while adhesive and surfactant properties are retained.
EP95938187A 1994-11-21 1995-09-29 Chemical modification of repetitive polymers to enhance water solubility Expired - Lifetime EP0792357B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US343264 1994-11-21
US08/343,264 US5760004A (en) 1994-11-21 1994-11-21 Chemical modification of repetitive polymers to enhance water solubility
PCT/US1995/012959 WO1996016168A1 (en) 1994-11-21 1995-09-29 Chemical modification of repetitive polymers to enhance water solubility

Publications (3)

Publication Number Publication Date
EP0792357A1 EP0792357A1 (en) 1997-09-03
EP0792357A4 true EP0792357A4 (en) 1999-05-19
EP0792357B1 EP0792357B1 (en) 2005-08-10

Family

ID=23345377

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95938187A Expired - Lifetime EP0792357B1 (en) 1994-11-21 1995-09-29 Chemical modification of repetitive polymers to enhance water solubility

Country Status (8)

Country Link
US (2) US5760004A (en)
EP (1) EP0792357B1 (en)
JP (1) JP3121019B2 (en)
AT (1) ATE301711T1 (en)
AU (1) AU697605B2 (en)
CA (1) CA2205219A1 (en)
DE (1) DE69534373T2 (en)
WO (1) WO1996016168A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010021513A1 (en) * 1991-11-21 2001-09-13 Puijk Wouter Cornelis Test device comprising a plate containing a multiplicity of wells with an associated metering device, as well as a kit which comprises these devices and use of the devices
JP2002281964A (en) * 2000-12-26 2002-10-02 Sanyo Chem Ind Ltd Cell production method
EP1448089A4 (en) * 2001-11-01 2008-06-04 Spine Wave Inc Devices and methods for the restoration of a spinal disc
WO2003037165A2 (en) * 2001-11-01 2003-05-08 Boyd Lawrence M System and method for the pretreatment of the endplates of an intervertebral disc
US7985601B2 (en) * 2002-03-08 2011-07-26 The Regents Of The University Of California Tunable, semi-interpenetrating polymer networks (sIPNS) for medicine and biotechnology
US8298606B2 (en) 2002-03-08 2012-10-30 The Regents Of The University Of California Methods and compositions for stabilizing the myocardium
AU2003233579A1 (en) * 2002-05-20 2003-12-12 Dow Corning Corporation Synthesis of inorganic structures using templation and catalysis by self assembled repeat protein polymers
US20040132978A1 (en) * 2002-11-12 2004-07-08 Fahnestock Stephen R. Method for purifying and recovering silk proteins in soluble form and uses thereof
JP2004206910A (en) * 2002-12-24 2004-07-22 Sumitomo Wiring Syst Ltd Fuse connector
US7060260B2 (en) * 2003-02-20 2006-06-13 E.I. Du Pont De Nemours And Company Water-soluble silk proteins in compositions for skin care, hair care or hair coloring
US20050142094A1 (en) * 2003-03-12 2005-06-30 Manoj Kumar Use of repeat sequence protein polymers in personal care compositions
US7297678B2 (en) * 2003-03-12 2007-11-20 Genencor International, Inc. Use of repeat sequence protein polymers in personal care compositions
WO2004104020A2 (en) * 2003-05-14 2004-12-02 Dow Corning Corporation Repeat sequence protein polymer active agent conjugates, methods and uses
JP4850709B2 (en) * 2003-05-14 2012-01-11 ダニスコ・ユーエス・インコーポレーテッド Controlled release of active agent using repetitive sequence protein polymer
TWI232934B (en) * 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
JP2008504895A (en) 2004-06-29 2008-02-21 スパイン・ウェイブ・インコーポレーテッド Method for treating disc defects and injuries
EP1869169A4 (en) * 2005-03-29 2015-11-04 Univ California Controlling stem cell destiny destiny with tunable networks
EP2636415B1 (en) 2010-11-04 2019-12-25 Sanyo Chemical Industries, Ltd. Cell adhesive material for biological tissue
US9700425B1 (en) 2011-03-20 2017-07-11 Nuvasive, Inc. Vertebral body replacement and insertion methods
DK3182985T3 (en) 2014-08-20 2020-04-06 Silk Tech Ltd FIBROINA FLATED PROTEIN COMPOSITION
CN107405277B (en) 2014-12-02 2021-08-10 丝绸医疗公司 Silk performance garments and products and methods of making same
US11512425B2 (en) 2015-07-14 2022-11-29 Evolved By Nature, Inc. Silk performance apparel and products and methods of preparing the same
SG11201808804TA (en) 2016-04-08 2018-11-29 Univ Cornell A method to enhance wound healing using silk-derived protein
AU2017310520A1 (en) 2016-08-12 2019-03-21 Silk Technologies, Ltd. Silk-derived protein for treating inflammation
US11390988B2 (en) 2017-09-27 2022-07-19 Evolved By Nature, Inc. Silk coated fabrics and products and methods of preparing the same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1904108A1 (en) * 1968-01-30 1970-07-23 Kyowa Hakko Kogyo Kk Hydroxyalkylated protein and process for its preparation
FR2063335A5 (en) * 1969-10-03 1971-07-09 Allemand Claude Proteins esterified with epoxides for cos-metics
US4224219A (en) * 1978-11-15 1980-09-23 Anheuser-Busch, Incorporated Method for producing water soluble corn protein derivatives by reacting with alkylene oxide
WO1990005177A1 (en) * 1988-11-09 1990-05-17 Syntro Corporation Functional recombinantly prepared synthetic protein polymer

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5243038A (en) * 1986-11-04 1993-09-07 Protein Polymer Technologies, Inc. Construction of synthetic DNA and its use in large polypeptide synthesis
DE4016002A1 (en) * 1990-05-18 1991-11-21 Basf Ag USE OF WATER-SOLUBLE OR WATER-DISPERSIBLE PEPPER PROTEINS AS ADDITION TO WASHING AND CLEANING AGENTS

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1904108A1 (en) * 1968-01-30 1970-07-23 Kyowa Hakko Kogyo Kk Hydroxyalkylated protein and process for its preparation
FR2063335A5 (en) * 1969-10-03 1971-07-09 Allemand Claude Proteins esterified with epoxides for cos-metics
US4224219A (en) * 1978-11-15 1980-09-23 Anheuser-Busch, Incorporated Method for producing water soluble corn protein derivatives by reacting with alkylene oxide
WO1990005177A1 (en) * 1988-11-09 1990-05-17 Syntro Corporation Functional recombinantly prepared synthetic protein polymer

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
APPELLO J.: "Genetic Production of Synthetic Protein Polymers", NRS BULLETIN, vol. 17, no. 10, 1992, pages 48 - 53, XP002096657 *
CANELLA M. ET AL.: "Functional and Physicochemical Properties of Succinylated and Acetylated Sunflower Protein", LEBENSM.-WISS., vol. 12, no. 2, 1979, pages 95 - 101, XP002096656 *

Also Published As

Publication number Publication date
JP3121019B2 (en) 2000-12-25
DE69534373T2 (en) 2006-05-24
US6034220A (en) 2000-03-07
EP0792357B1 (en) 2005-08-10
JPH10500701A (en) 1998-01-20
EP0792357A1 (en) 1997-09-03
AU697605B2 (en) 1998-10-15
CA2205219A1 (en) 1996-05-30
WO1996016168A1 (en) 1996-05-30
US5760004A (en) 1998-06-02
DE69534373D1 (en) 2005-09-15
ATE301711T1 (en) 2005-08-15
AU3891595A (en) 1996-06-17

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