EP1614466A3 - System and method to prevent air bubbles in a hybridization chamber - Google Patents

System and method to prevent air bubbles in a hybridization chamber Download PDF

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Publication number
EP1614466A3
EP1614466A3 EP05105498A EP05105498A EP1614466A3 EP 1614466 A3 EP1614466 A3 EP 1614466A3 EP 05105498 A EP05105498 A EP 05105498A EP 05105498 A EP05105498 A EP 05105498A EP 1614466 A3 EP1614466 A3 EP 1614466A3
Authority
EP
European Patent Office
Prior art keywords
hybridization
pressure
chamber
slides
agitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP05105498A
Other languages
German (de)
French (fr)
Other versions
EP1614466A2 (en
Inventor
Wolfgang Streit
Gyoergy Wenczel
Waltraud Lamprecht
Heribert Egelauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Trading AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE202004012163U external-priority patent/DE202004012163U1/en
Priority claimed from US10/931,432 external-priority patent/US7615370B2/en
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Priority to EP10172196.7A priority Critical patent/EP2255880B1/en
Publication of EP1614466A2 publication Critical patent/EP1614466A2/en
Publication of EP1614466A3 publication Critical patent/EP1614466A3/en
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Die Erfindung betrifft ein System (1) mit Hybridisierkammern (5) zum Hybridisieren von auf Objektträgern (27) immobilisierten Nukleinsäureproben, Proteinen oder Gewebeschnitten, wobei jede Hybridisierkammer (5) als ein im wesentlichen spaltförmiger, mit einer Flüssigkeit im wesentlichen befüllbarer Raum zwischen einem dieser Objektträger (27) und einem Deckel (26) definiert ist, wobei der Deckel (26) derart gegenüber dem Objektträger (27) angeordnet ist, dass die Hybridisierkammer (5) gegenüber der Umgebungsluft abgedichtet ist, und wobei das System (1) eine Agitationseinrichtung (32) umfasst, mit welcher Flüssigkeiten in den Hybridisierkammern (5) gegenüber den auf den Objektträgern (27) immobilisierten Proben bewegt werden können. Das erfindungsgemässe System ist dadurch gekennzeichnet, dass es eine Einrichtung zum Verhindern von Luftblasen in den Hybridisierkammern (5) umfasst, welche als Druckeinrichtung zum Aufbauen eines Kammer-Drucks in den Hybridisierkammern (5) ausgebildet ist, wobei dieser Kammer-Druck dadurch unabhängig von Agitationsdruckunterschieden über dem in der Umgebungsluft herrschenden, normalen atmosphärischen Druck liegt, dass das System (1) ein Federelement umfasst, welches mit dem für die Hybridisierung verwendeten Flüssigkeitsvolumen in Verbindung steht und federnd den durch die Agitationseinrichtung (32) auf den Druckraum (34) ausgeübten Agitationsdruckunterschieden entgegenwirkt.

Figure imgaf001
The invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as a substantially gap-shaped, substantially liquid-filled space between one of these Slides (27) and a lid (26) is defined, wherein the lid (26) is disposed opposite to the slide (27), that the hybridization chamber (5) is sealed from the ambient air, and wherein the system (1) an agitation device (32), with which liquids in the hybridization chambers (5) can be moved relative to the samples immobilized on the slides (27). The inventive system is characterized in that it comprises means for preventing air bubbles in the Hybridisierkammern (5), which is designed as a pressure device for establishing a chamber pressure in the Hybridisierkammern (5), said chamber pressure thereby independent of Agitationsdruckunterschieden above the normal atmospheric pressure prevailing in the ambient air, the system (1) comprises a spring member which communicates with the volume of liquid used for the hybridization and resiliently differentiates the agitation pressure applied by the agitation means (32) to the pressure space (34) counteracts.
Figure imgaf001
EP05105498A 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber Ceased EP1614466A3 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10172196.7A EP2255880B1 (en) 2005-06-21 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH11442004 2004-07-08
DE202004012163U DE202004012163U1 (en) 2004-07-08 2004-08-04 System for hybridizing immobilized nucleic acid samples, in sealed hybridizing chambers, has a pressure assembly to build up the chamber pressures over ambient atmospheric levels to prevent the formation of air bubbles
US10/931,432 US7615370B2 (en) 2004-07-08 2004-09-01 System having device for preventing air bubbles in a hybridization chamber and corresponding method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP10172196.7A Division EP2255880B1 (en) 2005-06-21 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Publications (2)

Publication Number Publication Date
EP1614466A2 EP1614466A2 (en) 2006-01-11
EP1614466A3 true EP1614466A3 (en) 2006-05-10

Family

ID=35106752

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05105498A Ceased EP1614466A3 (en) 2004-07-08 2005-06-21 System and method to prevent air bubbles in a hybridization chamber

Country Status (2)

Country Link
EP (1) EP1614466A3 (en)
JP (1) JP4819421B2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008098094A1 (en) * 2007-02-06 2008-08-14 Network Biosystems, Inc. Devices and methods for the performance of miniaturized in vitro assays
WO2009011942A2 (en) 2007-04-04 2009-01-22 Network Biosystems, Inc. Methods for rapid multiplexed amplification of target nucleic acids
CH699853A1 (en) 2008-11-13 2010-05-14 Tecan Trading Ag Meter and method for determining provided by a laboratory fluid system parameters.
EP2443254A2 (en) 2009-06-15 2012-04-25 NetBio, Inc. Improved methods for forensic dna quantitation
AT508703B1 (en) * 2009-07-15 2011-06-15 Ait Austrian Inst Technology SAMPLE CARRIER
US9132423B2 (en) 2010-01-29 2015-09-15 Micronics, Inc. Sample-to-answer microfluidic cartridge
KR102090934B1 (en) * 2012-01-09 2020-03-19 퍼킨엘머 헬스 사이언시즈, 아이엔씨. Microfluidic reactor system
KR20150097764A (en) 2012-12-21 2015-08-26 마이크로닉스 인코포레이티드. Portable fluorescence detection system and microassay cartridge
CN104919191B (en) 2012-12-21 2019-07-09 精密公司 Fluid circuit and relevant manufacturing method
EP3549674B1 (en) 2012-12-21 2020-08-12 PerkinElmer Health Sciences, Inc. Low elasticity films for microfluidic use
US10087440B2 (en) 2013-05-07 2018-10-02 Micronics, Inc. Device for preparation and analysis of nucleic acids
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
AU2016271049B9 (en) * 2015-05-29 2019-07-18 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
SG11201805707VA (en) 2016-01-05 2018-07-30 Nippon Sheet Glass Company Limited Reaction treatment device, reaction treatment container, and reaction treatment method
CN112683801B (en) * 2021-01-21 2023-04-28 上海菁一科技有限公司 Spectrophotometry sample treatment test capsule
CN114289091B (en) * 2022-01-18 2023-03-28 四川沃文特生物技术有限公司 Buffer tank for refrigeration system of automatic analysis equipment

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6684966B2 (en) * 2001-10-18 2004-02-03 Baker Hughes Incorporated PCD face seal for earth-boring bit

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009015A1 (en) * 1998-10-28 2002-01-24 Laugharn James A. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US20020075363A1 (en) * 2000-05-15 2002-06-20 Mcneely Michael R. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
EP1260265A1 (en) * 2001-05-25 2002-11-27 Tecan Trading AG Apparatus for the preparation of a hybridisation chamber, processing set and system for the hybridisation of samples of nucleic acid, proteines and tissues
WO2004052527A1 (en) * 2002-12-11 2004-06-24 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Method and reactor for the amplification of dna

Also Published As

Publication number Publication date
EP1614466A2 (en) 2006-01-11
JP4819421B2 (en) 2011-11-24
JP2006084459A (en) 2006-03-30

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