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Definitions

• the present invention relates to novel conformationally-defined macrocyclic compounds that bind to and/or are functional modulators of the motilin receptor including subtypes, isoforms and/or variants thereof.
• These macrocyclic compounds possess appropriate pharmacological properties to be useful as therapeutics for a range of disease indications.
• these compounds are useful for treatment and prevention of disorders characterized by hypermotilenemia or gastrointestinal hypermotility, including, but not limited to diarrhea, cancer treatment-related diarrhea, cancer-induced diarrhea, chemotherapy-induced diarrhea, radiation enteritis, radiation-induced diarrhea, stress-induced diarrhea, chronic diarrhea, AIDS-related diarrhea, C .
• the compounds possess utility for the treatment of diseases and disorders characterized by poor stomach or intestinal absorption, such as short bowel syndrome, celiac disease and cachexia.
• the compounds may also be used to treat inflammatory diseases and disorders of the gastrointestinal tract, such as inflammatory bowel disease, ulcerative colitis, Crohn's disease and pancreatitis.
• GI gastrointestinal
• absorption, secretion, blood flow and motility Mulvihill, S.J.; et al. in Basic and Clinical Endocrinology, 4th edition, Greenspan, F.S.; Baxter, J.D., Eds., Appleton & Lange: Norwalk, CT, 1994, pp 551-570 ). Since interactions between the brain and GI system are critical to the proper modulation of these functions, these peptides can be produced locally in the GI tract or distally in the CNS.
• motilin a linear 22-amino acid peptide
• motilin plays a critical regulatory role in the GI physiological system through governing of fasting gastrointestinal motor activity.
• the peptide is periodically released from the duodenal mucosa during fasting in mammals, including humans. More precisely, motilin exerts a powerful effect on gastric motility through the contraction of gastrointestinal smooth muscle to stimulate gastric emptying, decrease intestinal transit time and initiate phase III of the migrating motor complex (MMC) in the small bowel.
• MMC migrating motor complex
• Motilin can exert these effects through receptors located predominantly on the human antrum and proximal duodenum, although its receptors are found to some degree along the entire GI tract.
• Motilin receptors in the brain have been suggested to play a regulatory role in a number of CNS functions, including feeding and drinking behavior, micturition reflex, central and brain stem neuronal modulation, and pituitary hormone secretion ( Itoh, Z. Peptides 1997, 18, 593-608 ; Asakawa, A.; Inui, A.; Momose, K.M.; et al. Peptides 1998, 19, 987-990 and Rosenfeld, D.J.; Garthwaite, T.L. Physiol. Behav.
• motilin agonists as therapeutic agents to enhance.motility.
• the first of these, peptidic agonists of the motilin receptor have clinical application for the treatment of hypomotility disorders, in particular gastroparesis, ( Haramura, M.; Tsuzuki, K.; Okamachi, A.; et al. Bioorg. Med. Chem. 2002, 10, 1805-1811 ; U.S. Pat. Nos.
• the macrolide antibiotic erythromycin has long been known to have stimulation of GI motility as a side effect and, hence, has been utilized as a treatment for gastroparesis. This effect has subsequently been shown to be mediated through interaction at the motilin receptor.
• ABT-229 (alemcinal, Talley, N.J.; Verlinden, M.; Snape, W.; et al. Aliment. Pharmacol. Ther. 2000, 14, 1653-1661 ; Talley, N.J.; Verlinden, M.; Geenan, D.J.; et al. Gut 2001, 49, 395-401 ; Chen, C.L.; Orr, W.C.; Verlinden, M.H.; et al. Aliment. Pharmacol.
• Gastroenterology 2005, 128, A464 ; Carreras, C.W.; Burlingame, M.; Carney, J.; et al. Can. J. Gastroenterol. 2005,19, 15 ) has been described and appears to circumvent many of these problems.
• a method useful for analyzing the therapeutic efficiency of these types of molecules has also been formulated ( U.S. Pat. No. 6,875,576 ; U.S. Pat. Appl. Publ. 2002/192709 ; Intl. Pat. Appl. Publ. WO 02/64092 ).
• non-peptide, non-motilide motilin agonists have been reported ( U.S. Pat. No. 7,262,195 ; U.S. Pat. Appl. Publ. No. 2004/152732 ; 2005/065156 ; Intl. Pat. Appl. Publ. WO 02/137127 ; WO 02/92592 ; WO 2005/027908 ; WO 2005/027637 ; Jap. Pat. Abstr. Publ. No. 09249620 ).
• BMS-591348 has been described as possessing a pharmacological profile that avoids the tachyphylaxis issues that plagued many of the previous motilin agonist efforts.
• antagonists of the motilin receptor are potentially useful as therapeutic treatments for diseases associated with hypermotilinemia and/or gastrointestinal hypermotility, including diarrhea, cancer treatment-related diarrhea, cancer-induced diarrhea, chemotherapy-induced diarrhea, radiation enteritis, radiation-induced diarrhea, stress-induced diarrhea, chronic diarrhea, AIDS-related diarrhea, C . difficile associated diarrhea, traveller's diarrhea, diarrhea induced by graph versus host disease, other types of diarrhea, dyspepsia, irritable bowel syndrome, functional gastrointestinal disorders, chemotherapy-induced nausea and vomiting (emesis) and post-operative nausea and vomiting. Current treatments for these conditions are ineffective in many cases. Loperamide, an opioid agonist, is useful for milder diarrhea and generally does not work in a high percentage of patients.
• Octreotide a somatostatin agonist
• Diarrhea is a common and serious side-effect experienced by cancer patients resulting from surgery, bone marrow transplantation, chemotherapy and radiation treatment.
• chemotherapeutic regimens particularly those including fluoropyrimidines and irinotecan, result in chemotherapy-induced diarrhea (CID) rates as high as 50-80%.
• CID chemotherapy-induced diarrhea
• Acute radiation enteritis (ARE) or radiation induced intestinal dysfunction occurs in 75% of patients undergoing radiation therapy, typically occurring in the second or third week of therapy. Characterized by abdominal cramping and diarrhea, this is a serious and feared side effect that results in increased overall treatment time as well as reduced quality of life and can even result in death. In 5-15% of patients, the condition becomes chronic. In addition to discomfort, this side effect decreases the therapeutic benefit from radiation treatment by increasing the overall treatment time.
• chronic diarrhea can arise as a result of numerous medical conditions.
• chronic diarrhea is a common problem for patients with human immunodeficiency virus infection, especially those with advanced disease. This is a debilitating side effect that occurs in 60-90% of AIDS patients.
• Traveller's diarrhea affects over 50% of travellers to some destinations, particularly tropical ones, and is estimated to afflict over 11 million individuals annually. Apart from the disruption to business, travel and vacation schedules, this condition is often accompanied by other clinical manifestations such as nausea, vomiting, abdominal pain, fecal urgency, bloody stools, and fever.
• Lima A.A.M. Curr. Opin. Infect. Dis. 2001, 14, 547-552 ; Al-Abri, S.S.; Beeching, N.J.; Nye, F.J. Lancet Infect. Dis. 2005, 5, 349-360 ; DuPont, H.L. Gastroenterol. Clin. North Am. 2006, 35, 337-353 .
• Clostridium difficile is the etiological agent responsible for about one-third of cases of antibiotic-associated diarrhea and is estimated to cause a $1 billion annual cost in the U.S. Antibiotic-associated diarrhea is more common in the hospital setting with up to 29% of patients developing the condition, resulting in increased length of stay, increased cost of care, and increased mortality.
• GVHD graft versus host disease
• IBS Irritable bowel syndrome
• IBS patients are sub-classified into diarrhea-predominant (IBS-d), constipation-predominant (IBS-c) or those alternating between these two patterns (IBS-m).
• Antispasmodics, tricyclic antidepressants, selective serotonin reuptake inhibitors, laxatives, antidiarrheals, and bulking agents have not proven to be widely effective and tend to treat symptoms, rather than underlying pathophysiology.
• dyspepsia Another extremely common GI disorder, dyspepsia, is characterized by chronic or recurrent upper GI distress with no obvious physical cause.
• Typical symptoms include gastric fullness, bloating, pain, nausea and vomiting. This disease has prevalence as high as 20% annually in Western countries. It accounts for up to 5% of all visits to primary care physicians and 30% of visits to GI specialists.
• the patient population can be categorized into various subsets based upon symptoms.
• the largest patient subset (up to 60%) suffers from dyspepsia with no known organic cause, otherwise known as "functional dyspepsia (FD)." FD has a major impact on quality of life and health care resources.
• FD functional dyspepsia
• Circulating plasma motilin levels are also seen to be raised in patients suffering from dyspepsia.
• Chemotherapy-induced nausea and vomiting is one of the most severe adverse effects resulting from cancer treatment and is often cited as the side effect most feared by patients. From 70-80% of patients receiving cancer chemotherapy experience CINV. In addition to a significant deterioration in quality of life, this condition often requires modification or delay of chemotherapeutic regimens with concomitant negative impact on the effectiveness of treatment.
• CINV Chemotherapy-induced nausea and vomiting
• PONV Post-operative nausea and vomiting
• motilin In addition to its actions on motility, motilin is involved in inducing secretion within the gastrointestinal tract. Motilin plays a role in gastric and pancreatic secretion in dogs.
• motilin antagonists In addition to treatment of disorders characterized by hypermotility, the use of motilin antagonists would also be useful in the treatment of diseases and disorders characterized by poor stomach or intestinal absorption. A motilin antagonist would slow gastrointestinal motility thereby permitting longer GI exposure time for absorption of necessary nutrients.
• diseases and disorders include celiac disease, a chronic disorder afflicting almost 1% of the population.
• Celiac disease is a GI disorder characterized by inflammation, leading to injury to the mucosal lining of the small intestine. The inflammation results when gliadin, a protein found in gluten-containing foods, is ingested by genetically susceptible individuals. The mucosal damage and subsequent malabsorption of nutrients can lead to numerous complications.
• Short bowel syndrome is a medical condition that occurs after resection of a substantial portion of small intestine and is characterized by malnutrition.
• cancer cachexia is a therapeutic condition characterized by weight loss and muscle wasting and afflicts approximately 50% of all cancer patients and is the main cause of death in more than 20% of patients. Additionally, this condition has been shown to be a strong independent risk factor for mortality.
• motilin has been implicated in inflammatory disorders of the GI system. Elevated motilin levels have been observed in patients with inflammatory bowel disease ( Besterman, H.S.; Mallinson, C.N.; et al. Scand. J. Gastroenterol. 1983, 18, 845-852 ), ulcerative colitis ( Greenberg, G.R.; Buchan, A.M.; McLeod, R.S.; Preston, P.; Cohen, Z. Gut 1989, 30, 1721-1730 ) and chronic pancreatitis ( Besterman, H.S.; Adrian, T.E.; Bloom, S.R. et al.
• motilin antagonists may be used as anti-inflammatory agents, in particular for use in the GI tract, with beneficial properties relative to existing treatments.
• motilin antagonists As a novel approach to treat hypermotility and malabsorption disorders, efforts have lagged those directed at agonists.
• a variety of peptidic compounds have been described as antagonists of the motilin receptor [(ANQ-11125: Peeters, T.L.; Depoortere, I.; Macielag, M.J.; Marvin, M.S.; Florance, J.R.; Galdes, A. Biochem. Biophys. Res. Comm.
• Cyclization of peptidic derivatives is a method that can be employed to improve the properties of a linear peptide both with respect to metabolic stability and conformational freedom. Cyclic molecules tend to be more resistant to metabolic enzymes. Such cyclic peptide motilin antagonists have been reported, highlighted by GM-109. ( Takanashi, H.; Yogo, K.; Ozaki, M.; Akima, M.; Koga, H.; Nabata, H. J. Pharm. Exp. Ther.
• Macrocyclic peptidomimetics have been previously described as antagonists of the motilin receptor and their uses for the treatment of a variety of GI disorders and to modulate the migrating motor complex summarized.
• peptidomimetic macrocyclic motilin antagonists are distinguished from the aforementioned cyclic peptide motilin antagonists in that it was found that such peptidic derivatives containing D-amino acids were devoid of activity.
• the D-stereochemistry is beneficial for two of the three building elements.
• the tether portion of the molecule provides a non-peptidic component and, hence, distinct structures.
• the peptidomimetic macrocycles of the present invention are demonstrated to have binding and functional activity at the motilin receptor.
• binding potency and target affinity are factors in drug discovery and development, also important for development of viable pharmaceutical agents are optimization of pharmacokinetic (PK) and pharmacodynamic (PD) parameters.
• PK pharmacokinetic
• PD pharmacodynamic
• the macrocyclic compounds of the present invention have been found to possess desirable pharmacological characteristics, while maintaining sufficient binding affinity and selectivity for the motilin receptor, as illustrated in the Examples. These combined characteristics make them more suitable for development as pharmaceutical agents.
• the present invention provides novel conformationally-defined macrocyclic compounds with improved pharmacological properties. These compounds can function as antagonists of the motilin receptor.
• the present invention is directed to compounds of formula I: and pharmaceutically acceptable salts, hydrates or solvates thereof wherein: Y is wherein (L 5 ) and (L 6 ) indicates the bonds to L 5 and L 6 of formula I, respectively;
• Ar of formula I is selected from:
• R 1 of formula I is selected from methyl, ethyl, isopropyl and cyclopropyl.
• R 2 of formula I is selected from: wherein M is selected from the group consisting of CH 2 , O, NH, and NCH 3 .
• L 1 , L 2 , L 3 and L 4 in formula I are each CH, L 5 is O and L 6 is CH 2 .
• R 3 , R 4 , R 5 and R 6 in formula I are each hydrogen; or R 3 is methyl and R 4 , R 5 and R 6 are each hydrogen; or R 3 is hydroxymethyl and R 4 , R 5 and R 6 are each hydrogen; or R 3 , R 4 and R 6 are each hydrogen and R 5 is methyl; or R 3 and R 5 are each methyl and R 4 and R 6 are each hydrogen.
• Y in formula I is selected from: wherein (L 5 ) and (L 6 ) indicate the bond to L 5 and L 6 , respectively.
• the present invention relates to compounds of formula II: wherein R 25 is selected from hydrogen, alkyl, aryl, cycloalkyl, heterocyclic and heteroaryl; R 26 is selected from hydrogen, alkyl, aryl, acyl, carboxyalkyl, carboxyaryl, sulfonyl and a standard protecting group used for amino acids; R 27 is selected from the group consisting of hydrogen and alkyl; and R 28 is selected from the group consisting of wherein M is selected from the group consisting of CH 2 , O, NH, and NCH 3 .
• the present invention relates to compounds of formula III: wherein X 10 and X 11 are independently selected from the group consisting of hydrogen, halogen, trifluoromethyl and lower alkyl; R 40 , R 41 , R 42 , and R 43 are independently selected from the group consisting of hydrogen, lower alkyl and substituted lower alkyl; R 44 is selected from the group consisting of hydrogen, alkyl, acyl, carboxyalkyl, carboxyaryl, sulfonyl and a protecting group for an amine functional group; R 45 is selected from the group consisting of hydrogen and alkyl; R 46 is selected from the group consisting of hydrogen, alkyl, acyl, sulfonyl and a protecting group for a hydroxy functional group; L 11 , L 12 , L 13 and L 14 are independently selected from the group consisting of CH and N; with the proviso that the total number of nitrogens in the ring must be 0, 1, 2 or 3; L 15 and L 16 are independently selected from the group
• Compounds of formula III can be used in another aspect of the invention to provide compounds of formula I.
• Compounds of formula III can further be used to provide macrocyclic compounds including a building block structure, such as an amino acid building block structure, and the compounds of formula III.
• the present invention also relates to methods of using the compounds as described herein and as used for the preparation of a medicament for prevention and/or treatment of the disorders described herein.
• alkyl refers to straight or branched chain saturated or partially unsaturated hydrocarbon groups having from 1 to 20 carbon atoms, in some instances 1 to 8 carbon atoms.
• lower alkyl refers to alkyl groups containing 1 to 6 carbon atoms. Examples of alkyl groups include, but are not limited to, methyl, ethyl, isopropyl, tert-butyl, 3-hexenyl, and 2-butynyl.
• unsaturated is meant the presence of 1, 2 or 3 double or triple bonds, or a combination of the two. Such alkyl groups may also be optionally substituted as described below.
• C 2 -C 4 alkyl indicates an alkyl group with 2, 3 or 4 carbon atoms.
• cycloalkyl refers to saturated or partially unsaturated cyclic hydrocarbon groups having from 3 to 15 carbon atoms in the ring, in some instances 3 to 7, and to alkyl groups containing said cyclic hydrocarbon groups.
• examples of cycloalkyl groups include, but are not limited to, cyclopropyl, cyclopropylmethyl, cyclopentyl, 2-(cyclohexyl)ethyl, cycloheptyl, and cyclohexenyl.
• Cycloalkyl as defined herein also includes groups with multiple carbon rings, each of which may be saturated or partially unsaturated, for example decalinyl, [2.2.1]-bicycloheptanyl or adamantanyl. All such cycloalkyl groups may also be optionally substituted as described below.
• aromatic refers to an unsaturated cyclic hydrocarbon group having a conjugated pi electron system that contains 4n+2 electrons where n is an integer greater than or equal to 1.
• Aromatic molecules are typically stable and are depicted as a planar ring of atoms with resonance structures that consist of alternating double and single bonds, for example benzene or naphthalene.
• aryl refers to an aromatic group in a single or fused carbocyclic ring system having from 6 to 15 ring atoms, in some instances 6 to 10, and to alkyl groups containing said aromatic groups.
• aryl groups include, but are not limited to, phenyl, 1-naphthyl, 2-naphthyl and benzyl.
• Aryl as defined herein also includes groups with multiple aryl rings which may be fused, as in naphthyl and anthracenyl, or unfused, as in biphenyl and terphenyl.
• Aryl also refers to bicyclic or tricyclic carbon rings, where one of the rings is aromatic and the others of which may be saturated, partially unsaturated or aromatic, for example, indanyl or tetrahydronaphthyl (tetralinyl). All such aryl groups may also be optionally substituted as described below.
• heterocycle refers to saturated or partially unsaturated monocyclic, bicyclic or tricyclic groups having from 3 to 15 atoms, in some instances 3 to 7, with at least one heteroatom in at least one of the rings, said heteroatom being selected from O, S or N.
• Each ring of the heterocyclic group can contain one or two O atoms, one or two S atoms, one to four N atoms, provided that the total number of heteroatoms in each ring is four or less and each ring contains at least one carbon atom.
• the fused rings completing the bicyclic or tricyclic heterocyclic groups may contain only carbon atoms and may be saturated or partially unsaturated.
• heterocyclic also refers to alkyl groups containing said monocyclic, bicyclic or tricyclic heterocyclic groups. Examples of heterocyclic rings include, but are not limited to, 2- or 3-piperidinyl, 2- or 3-piperazinyl, 2- or 3-morpholinyl. All such heterocyclic groups may also be optionally substituted as described below
• heteroaryl refers to an aromatic group in a single or fused ring system having from 5 to 15 ring atoms, in some instances 5 to 10, which have at least one heteroatom in at least one of the rings, said heteroatom being selected from O, S or N.
• Each ring of the heteroaryl group can contain one or two O atoms, one or two S atoms, one to four N atoms, provided that the total number of heteroatoms in each ring is four or less and each ring contains at least one carbon atom.
• the fused rings completing the bicyclic or tricyclic groups may contain only carbon atoms and may be saturated, partially unsaturated or aromatic.
• the N atoms may optionally be quaternized or oxidized to the N-oxide.
• Heteroaryl also refers to alkyl groups containing said cyclic groups.
• Examples of monocyclic heteroaryl groups include, but are not limited to pyrrolyl, pyrazolyl, pyrazolinyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, isothiazolyl, furanyl, thienyl, oxadiazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, and triazinyl.
• bicyclic heteroaryl groups include, but are not limited to indolyl, benzothiazolyl, benzoxazolyl, benzothienyl, quinolinyl, tetrahydroisoquinolinyl, isoquinolinyl, benzimidazolyl, benzopyranyl, indolizinyl, benzofuranyl, isobenzofuranyl, chromonyl, coumarinyl, benzopyranyl, cinnolinyl, quinoxalinyl, indazolyl, purinyl, pyrrolopyridinyl, furopyridinyl, thienopyridinyl, dihydroisoindolyl, and tetrahydroquinolinyl.
• tricyclic heteroaryl groups include, but are not limited to carbazolyl, benzindolyl, phenanthrollinyl, acridinyl, phenanthridinyl, and xanthenyl. All such heteroaryl groups may also be optionally substituted as described below.
• hydroxy refers to the group -OH.
• alkoxy refers to the group -OR a , wherein R a is alkyl, cycloalkyl or heterocyclic. Examples include, but are not limited to methoxy, ethoxy, tert-butoxy, cyclohexyloxy and tetrahydropyranyloxy.
• aryloxy refers to the group -OR b wherein R b is aryl or heteroaryl. Examples include, but are not limited to phenoxy, benzyloxy and 2-naphthyloxy.
• amino acyl indicates an acyl group that is derived from an amino acid.
• amino refers to an -NR d R e group wherein R d and R e are independently selected from the group consisting of hydrogen, alkyl, cycloalkyl, heterocyclic, aryl and heteroaryl.
• R d and R e together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• R f and R g together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• R i and R j together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• Carboxyalkyl refers to the group -CO 2 R k , wherein R k is alkyl, cycloalkyl or heterocyclic.
• Carboxyaryl refers to the group -CO 2 R m , wherein R m is aryl or heteroaryl.
• cyano refers to the group -CN.
• halo refers to fluoro, fluorine or fluoride, chloro, chlorine or chloride, bromo, bromine or bromide, and iodo, iodine or iodide, respectively.
• hydroxymethyl refers to the group -CH 2 OH.
• mercapto refers to the group -SR n wherein R n is hydrogen, alkyl, cycloalkyl, heterocyclic, aryl or heteroaryl.
• nitro refers to the group -NO 2 .
• trifluoromethyl refers to the group -CF 3 .
• R r and R s together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• R x and Ry together form a heterocyclic ring or 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• R aa and R bb together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• optionally substituted is intended to expressly indicate that the specified group is unsubstituted or substituted by one or more suitable substituents, unless the optional substituents are expressly specified, in which case the term indicates that the group is unsubstituted or substituted with the specified substituents.
• various groups may be unsubstituted or substituted (i.e., they are optionally substituted) unless indicated otherwise herein (e.g., by indicating that the specified group is unsubstituted).
• R cc , R dd , R ee , R ff , R gg , R hh , R ii , R jj , R mm , R pp , R qq and R rr are independently selected from hydrogen, unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl or unsubstituted heteroaryl; and wherein R kk and R nn are independently selected from unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl or unsubstituted heteroaryl.
• R gg and R hh , R jj and R kk or R pp and Rqq together form a heterocyclic ring of 3 to 8 members, optionally substituted with unsubstituted alkyl, unsubstituted cycloalkyl, unsubstituted heterocyclic, unsubstituted aryl, unsubstituted heteroaryl, hydroxy, alkoxy, aryloxy, acyl, amino, amido, carboxy, carboxyalkyl, carboxyaryl, mercapto, sulfinyl, sulfonyl, sulfonamido, amidino, carbamoyl, guanidino or ureido, and optionally containing one to three additional heteroatoms selected from O, S or N.
• substituted for aryl and heteroaryl groups includes as an option having one of the hydrogen atoms of the group replaced by cyano, nitro or tri
• substitution is made provided that any atom's normal valency is not exceeded and that the substitution results in a stable compound.
• such substituted group is preferably not further substituted or, if substituted, the substituent comprises only a limited number of substituted groups, in some instances 1, 2, 3 or 4 such substituents.
• stable compound or “stable structure” refers to a compound that is sufficiently robust to survive isolation to a useful degree of purity and formulation into an efficacious therapeutic agent.
• amino acid refers to the common natural (genetically encoded) or synthetic amino acids and common derivatives thereof, known to those skilled in the art.
• standard or “proteinogenic” refers to the genetically encoded 20 amino acids in their natural configuration.
• unnatural or “unusual” refers to the wide selection of non-natural, rare or synthetic amino acids such as those described by Hunt, S. in Chemistry and Biochemistry of the Amino Acids, Barrett, G.C., Ed., Chapman and Hall: New York, 1985 .
• fragment with respect to a dipeptide, tripeptide or higher order peptide derivative indicates a group that contains two, three or more, respectively, amino acid residues.
• amino acid side chain refers to any side chain from a standard or unnatural amino acid, and is denoted R AA .
• the side chain of alanine is methyl
• the side chain of valine is isopropyl
• the side chain of tryptophan is 3-indolylmethyl.
• agonist refers to a compound that duplicates at least some of the effect of the endogenous ligand of a protein, receptor, enzyme or the like.
• antagonist refers to a compound that inhibits at least some of the effect of the endogenous ligand of a protein, receptor, enzyme or the like.
• variable when applied to a receptor is meant to include dimers, trimers, tetramers, pentamers and other biological complexes containing multiple components. These components can be the same or different.
• peptide refers to a chemical compound comprised of two or more amino acids covalently bonded together.
• peptidomimetic refers to a chemical compound designed to mimic a peptide, but which contains structural differences through the addition or replacement of one of more functional groups of the peptide in order to modulate its activity or other properties, such as solubility, metabolic stability, oral bioavailability, lipophilicity, permeability, etc. This can include replacement of the peptide bond, side chain modifications, truncations, additions of functional groups, etc.
• non-peptide peptidomimetic When the chemical structure is not derived from the peptide, but mimics its activity, it is often referred to as a "non-peptide peptidomimetic.”
• protecting group refers to any chemical compound that may be used to prevent a potentially reactive functional group, such as an amine, a hydroxyl or a carboxyl, on a molecule from undergoing a chemical reaction while chemical change occurs elsewhere in the molecule.
• a potentially reactive functional group such as an amine, a hydroxyl or a carboxyl
• a number of such protecting groups are known to those skilled in the art and examples can be found in " Protective Groups in Organic Synthesis," Theodora W. Greene and Peter G. Wuts, editors, John Wiley & Sons, New York, 3rd edition, 1999 [ISBN 0471160199 ].
• amino protecting groups include, but are not limited to, phthalimido, trichloroacetyl, benzyloxycarbonyl, tert-butoxycarbonyl, and adamantyloxycarbonyl.
• amino protecting groups are carbamate amino protecting groups, which are defined as an amino protecting group that when bound to an amino group forms a carbamate.
• amino carbamate protecting groups are allyloxycarbonyl (Alloc), benzyloxycarbonyl (Cbz), 9-fluorenylmethoxycarbonyl (Fmoc), tert-butoxycarbonyl (Boc) and ⁇ , ⁇ -dimethyl-3,5-dimethoxybenzyloxycarbonyl (Ddz).
• hydroxyl protecting groups include, but are not limited to, acetyl, tert-butyldimethylsilyl (TBDMS), trityl (Trt), tert-butyl, and tetrahydropyranyl (THP).
• carboxyl protecting groups include, but are not limited to methyl ester, tert-butyl ester, benzyl ester, trimethylsilylethyl ester, and 2,2,2-trichloroethyl ester.
• solid phase chemistry refers to the conduct of chemical reactions where one component of the reaction is covalently bonded to a polymeric material (solid support as defined below). Reaction methods for performing chemistry on solid phase have become more widely known and established outside the traditional fields of peptide and oligonucleotide chemistry.
• solid support refers to a mechanically and chemically stable polymeric matrix utilized to conduct solid phase chemistry. This is denoted by "Resin,” “P-” or the following symbol:
• polystyrene polyethylene, polyethylene glycol, polyethylene glycol grafted or covalently bonded to polystyrene (also termed PEG-polystyrene, TentaGelTM, Rapp, W.; Zhang, L.; Bayer, E. In Innovations and Perspectives in Solid Phase Synthesis. Peptides, Polypeptides and Oligonucleotides; Epton, R., Ed.; SPCC Ltd.: Birmingham, UK; p 205 ); polyacrylate (CLEARTM), polyacrylamide, polyurethane, PEGA [polyethyleneglycol poly(N,N-dimethylacrylamide) co-polymer, Meldal, M.
• polystyrene polyethylene, polyethylene glycol, polyethylene glycol grafted or covalently bonded to polystyrene
• PEG-polystyrene TentaGelTM, Rapp, W.; Zhang, L.; Bayer, E. In Innovations and Perspectives
• This solid support can include as non-limiting examples aminomethyl polystyrene, hydroxymethyl polystyrene, benzhydrylamine polystyrene (BHA), methylbenzhydrylamine (MBHA) polystyrene, and other polymeric backbones containing free chemical functional groups, most typically, -NH 2 or -OH, for further derivatization or reaction.
• the materials used as resins are insoluble polymers, but certain polymers have differential solubility depending on solvent and can also be employed for solid phase chemistry.
• polyethylene glycol can be utilized in this manner since it is soluble in many organic solvents in which chemical reactions can be conducted, but it is insoluble in others, such as diethyl ether.
• reactions can be conducted homogeneously in solution, then the product on the polymer precipitated through the addition of diethyl ether and processed as a solid. This has been termed "liquid-phase" chemistry.
• linker when used in reference to solid phase chemistry refers to a chemical group that is bonded covalently to a solid support and is attached between the support and the substrate typically in order to permit the release (cleavage) of the substrate from the solid support. However, it can also be used to impart stability to the bond to the solid support or merely as a spacer element. Many solid supports are available commercially with linkers already attached.
• the term "effective amount” or “effective” is intended to designate a dose that causes a relief of symptoms of a disease or disorder as noted through clinical testing and evaluation, patient observation, and/or the like, and/or a dose that causes a detectable change in biological or chemical activity.
• the detectable changes may be detected and/or further quantified by one skilled in the art for the relevant mechanism or process.
• the dosage will vary depending on the administration routes, symptoms and body weight of the patient but also depending upon the compound being administered.
• Administration of two or more compounds "in combination” means that the two compounds are administered closely enough in time that the presence of one alters the biological effects of the other.
• the two compounds can be administered simultaneously (concurrently) or sequentially. Simultaneous administration can be carried out by mixing the compounds prior to administration, or by administering the compounds at the same point in time but at different anatomic sites or using different routes of administration.
• the phrases "concurrent administration”, “administration in combination”, “simultaneous administration” or “administered simultaneously” as used herein, means that the compounds are administered at the same point in time or immediately following one another. In the latter case, the two compounds are administered at times sufficiently close that the results observed are indistinguishable from those achieved when the compounds are administered at the same point in time.
• pharmaceutically active metabolite is intended to mean a pharmacologically active product produced through metabolism in the body of a specified compound.
• solvate is intended to mean a pharmaceutically acceptable solvate form of a specified compound that retains the biological effectiveness of such compound.
• examples of solvates include compounds of the invention in combination with water, isopropanol, ethanol, methanol, DMSO, ethyl acetate, acetic acid, or ethanolamine.
• Novel macrocyclic compounds of the present invention include macrocyclic compounds comprising a building block structure including a tether component that undergoes cyclization to form the macrocyclic compound.
• the building block structure can comprise amino acids (standard and unnatural) and a tether component as described herein.
• Macrocyclic compounds of the present invention further include those of formula I: and pharmaceutically acceptable salts, hydrates or solvates thereof wherein: Y is wherein (L 5 ) and (L 6 ) indicates the bonds to L 5 and L 6 of formula I, respectively;
• Ar is selected from the group consisting of: R 1 is selected from the group consisting of lower alkyl and cycloalkyl; R 2 is selected from the group consisting of lower alkyl, substituted lower alkyl, cycloalkyl and substituted cycloalkyl; R 3 , R 4 , R 5 , and R 6 are independently selected from the group consisting of hydrogen, lower alkyl and substituted lower alkyl; R 7 is selected from the group consisting of hydrogen, lower alkyl, hydroxy and amino; R 10a and R 10b are independently selected from the group consisting of hydrogen, lower alkyl and substituted lower alkyl; X 1 , X 2 , X 6 , X 7 , X 8 and X 9
• the present invention includes isolated compounds.
• An isolated compound refers to a compound that, in some embodiments, comprises at least 10%, at least 25%, at least 50% or at least 70% of the compounds of a mixture.
• the compound, pharmaceutically acceptable salt thereof or pharmaceutical composition containing the compound exhibits a statistically significant binding and/or antagonist activity when tested in biological assays at the human motilin receptor.
• the compounds of formula I disclosed herein have asymmetric centers.
• the inventive compounds may exist as single stereoisomers, racemates, and/or mixtures of enantiomers and/or diastereomers. All such single stereoisomers, racemates, and mixtures thereof are intended to be within the scope of the present invention. In particular embodiments, however, the inventive compounds are used in optically pure form.
• the terms "S” and "R” configuration as used herein are as defined by the IUPAC 1974 Recommendations for Section E, Fundamentals of Stereochemistry ( Pure Appl. Chem. 1976, 45, 13-30 .)
• the compounds may be prepared as a single stereoisomer or a mixture of stereoisomers.
• the non-racemic forms may be obtained by either synthesis or resolution.
• the compounds may, for example, be resolved into the component enantiomers by standard techniques, for example formation of diastereomeric pairs via salt formation.
• the compounds also may be resolved by covalently bonding to a chiral moiety.
• the diastereomers can then be resolved by chromatographic separation and/or crystallographic separation. In the case of a chiral auxiliary moiety, it can then be removed.
• the compounds can be resolved through the use of chiral chromatography. Enzymatic methods of resolution could also be used in certain cases.
• an “optically pure” compound is one that contains only a single enantiomer.
• the term “optically active” is intended to mean a compound comprising at least a sufficient excess of one enantiomer over the other such that the mixture rotates plane polarized light.
• Optically active compounds have the ability to rotate the plane of polarized light. The excess of one enantiomer over another is typically expressed as enantiomeric excess (e.e.).
• the prefixes D and L or R and S are used to denote the absolute configuration of the molecule about its chiral center(s).
• the prefixes "d” and “I” or (+) and (-) are used to denote the optical rotation of the compound ( i.e. , the direction in which a plane of polarized light is rotated by the optically active compound).
• the "I” or (-) prefix indicates that the compound is levorotatory ( i.e. , rotates the plane of polarized light to the left or counterclockwise) while the "d” or (+) prefix means that the compound is dextrarotatory ( i.e. , rotates the plane of polarized light to the right or clockwise).
• the sign of optical rotation, (-) and (+) is not related to the absolute configuration of the molecule, R and S.
• a compound of the invention having the desired pharmacological properties will be optically active and, can be comprised of at least 90% (80% e.e.), at least 95% (90% e.e.), at least 97.5% (95% e.e.) or at least 99% (98% e.e.) of a single isomer.
• the compounds of formula I can be synthesized using traditional solution synthesis techniques or solid phase chemistry methods. Synthetic methods for this general type of macrocyclic structure are described in Intl. Pat. Appls. WO 01/25257 , WO 2004/111077 , WO 2005/012331 , WO 2005/012332 , WO 2006/009645 and WO 2006/009674 , including purification procedures described in WO 2004/111077 and WO 2005/012331 . Representative procedures for compounds of the invention are presented in the Examples.
• Reagents and solvents were of reagent quality or better and were used as obtained from various commercial suppliers unless otherwise noted.
• DMF, DCM (CH 2 Cl 2 ), DME, CH 3 CN and THF used are of DriSolv ® (EMD Chemicals, Inc., part of Merck KGaA, Darmstadt, Germany) or synthesis grade quality except for (i) deprotection, (ii) resin capping reactions and (iii) washing.
• NMP used for the amino acid (AA) coupling reactions is of analytical grade.
• DMF was adequately degassed by placing under vacuum for a minimum of 30 min prior to use. Homogeneous catalysts were obtained from Strem Chemicals, Inc. (Newbury Port, MA, USA).
• Cbz-, Boc- and Fmoc-protected amino acids and side chain protected derivatives, including those of N-methyl and unnatural amino acids, were obtained from commercial suppliers or synthesized through standard methodologies known to those in the art.
• Ddz-amino acids were either synthesized by standard methods, or obtained commercially from Orpegen (Heidelberg, Germany) or Advanced ChemTech (Louisville, KY, USA). Bts-amino acids were synthesized by established procedures.
• Analytical TLC was performed on pre-coated plates of silica gel 60F254 (0.25 mm thickness) containing a fluorescent indicator and were visualized using the method(s) and reagent(s) indicated, for example using ultraviolet light (UV) and/or ceric-molybdic acid (CMA) solution (prepared by mixing 100 mL of sulfuric acid, 10 g ceric ammonium sulfate and 25 g ammonium molybdate).
• UV ultraviolet light
• CMA ceric-molybdic acid
• concentrated/evaporated/removed under reduced pressure indicates removal of solvent and volatile components utilizing a rotary evaporator under either water aspirator pressure (typically 10-30 torr) or the stronger vacuum provided by a mechanical oil vacuum pump ("high vacuum,” typically ⁇ 1 torr) as appropriate for the solvent being removed. Drying of a compound “ in vacuo " or under “high vacuum” refers to drying using an oil vacuum pump at low pressure ( ⁇ 1 torr).
• “Flash chromatography” was performed using silica gel 60 (230-400 mesh, EMD Chemicals, Still, W. C.; Kahn, M.; Mitra, A. J. Org. Chem.
• “Dry pack” indicates chromatography on silica gel that has not been pre-treated with solvent, generally applied on larger scales for purifications where a large difference in R f exists between the desired product and any impurities.
• “dried in the standard manner” is that the resin is dried first in air (1 h), and subsequently under vacuum (oil pump usually) until full dryness is attained ( ⁇ 30 min to O/N). Glassware used in air and water sensitive reactions were dried in an oven at least O/N and cooled in a desiccator prior to use.
• Amino acids, Boc- and Fmoc-protected amino acids and side chain protected derivatives, including those of N-methyl and unnatural amino acids were obtained from commercial suppliers [for example: Advanced ChemTech (Louisville, KY, USA), Bachem (Bubendorf, Switzerland), ChemImpex (Wood Dale, IL, USA), Novabiochem (subsidiary of Merck KGaA, Darmstadt, Germany), PepTech (Burlington, MA, USA), Synthetech (Albany, OR, USA), AstaTech (Bristol, PA, USA)] or synthesized through standard methodologies known to those in the art.
• Ddz-amino acids were either obtained commercially from Orpegen (Heidelberg, Germany) or Advanced ChemTech (Louisville, KY, USA) or synthesized using standard methods utilizing Ddz-OPh or Ddz-N 3 .
• Bts-amino acids were synthesized by known methods.
• N-Alkyl amino acids in particular N-methyl amino acids, are commercially available from multiple vendors (Bachem, Novabiochem, Advanced ChemTech, ChemImpex, Peptech).
• N-alkyl amino acid derivatives were accessed via literature methods. ( Hansen, D. W., Jr.; Pilipauskas, D. J. Org. Chem. 1985, 50, 945-950 .)
• Aziridine-2-carboxylic acid was constructed using reported methods from the literature ( Baldwin, J. E. et al.
• R 25 is selected from hydrogen, alkyl, aryl, cycloalkyl, heterocyclic and heteroaryl
• R 26 is selected from hydrogen, alkyl, aryl, acyl, carboxyalkyl, carboxyaryl, sulfonyl and a standard protecting group used for amino acids
• R 27 is selected from the group consisting of hydrogen and alkyl
• R 28 is selected from the group consisting of wherein M is selected from the group consisting of CH 2 , O, NH, and NCH 3 .
• Tethers were obtained from the methods previously described in Intl. Pat. Appl. Nos. WO 01/25257 , WO 2004/111077 , WO 2005/012331 , WO 2006/009645 and WO 2006/009674 . Procedures for the synthesis of representative tethers as described herein are also presented in the Examples below. Tethers useful for the compounds of the present invention can include suitable tethers as described in the references cited above as well as known and/or novel tethers described herein.
• Exemplary tethers include, but are not limited to, the following: and intermediates in the manufacture thereof, wherein PG 1 is selected from hydrogen and a protecting group for an amine functional group and PG 2 is selected from hydrogen and a protecting group for a hydroxy functional group.
• (R)-substituted tether building blocks result in compounds of formula I with an (S)-stereocenter.
• (S)-substituted tether building blocks result in compounds of formula I with an (R)-stereocenter.
• Tethers T90 and T91 are introduced along with AA 1 as fragments F2 and F1 respectively as described in Examples 6 and 5.
• the relevant deprotection and coupling protocols required for the assembly of the cyclization precursors are performed utilizing standard procedures and those described in WO 2004/111077 , WO 2006/009645 and WO 2006/009674 as appropriate for the nature of the building block.
• the final macrocycles are obtained after application of the appropriate deprotection sequences.
• HPLC analyses were performed on a Waters Alliance ® system 2695 running at 1 mL/min using an Xterra ® MS C18 column (or comparable) 4.6 x 50 mm (3.5 ⁇ m) and the indicated gradient method.
• a Waters 996 PDA provided UV data for purity assessment (Waters Corporation, Milford, MA).
• the second part (40%) went to an evaporative light scattering detector (ELSD, Polymer Laboratories, now part of Varian, Inc., Palo Alto, CA, PL-ELS-1000TM) for purity assessment and the last portion (10%) went to a chemiluminescence nitrogen detector (CLND, Antek ® Model 8060, Antek Instruments, Houston, TX, part of Roper Industries, Inc., Duluth, GA) for quantitation and purity assessment. Data was captured and processed utilizing the most recent version of the Waters Millennium ® software package.
• ELSD evaporative light scattering detector
• CLND chemiluminescence nitrogen detector
• Enantiomeric and diastereomeric purity were assessed using appropriate chiral HPLC columns using a Waters Breeze system (or comparable). Although other packing materials can be utilized, particularly useful columns for these analyses are: Chiralpak AS-RH and Chiralcel OD-RH (Chiral Technologies, West Chester, PA, USA).
• Preparative HPLC purifications were performed on final deprotected macrocycles using the Waters FractionLynx ® system, on an XTerra ® MS C18 column (or comparable) 19 x 100 mm (5 ⁇ m). The injections were done using an At-Column-Dilution configuration with a Waters 2767 injector/collector and a Waters 515 pump running at 2 mL/min. The mass spectrometer, HPLC, and mass-directed fraction collection are controlled via MassLynx ® software version 3.5 with FractionLynx ® .
• the compounds of the present invention were evaluated for their ability to interact at the human motilin receptor utilizing a competitive radioligand binding assay, fluorescence assay or Aequorin functional assay as described below. Such methods can be conducted in a high throughput manner to permit the simultaneous evaluation of many compounds.
• the competitive binding assay at the human motilin receptor was carried out analogously to assays described in the literature.
• the reaction is initiated by addition of 10 ⁇ L of [ 125 I]-motilin (final conc. 0.04 - 0.06 nM) to each well. Plates are sealed with TopSeal-A, vortexed gently and incubated at room temperature for 2 hours.
• the reaction is arrested by filtering samples through pre-soaked (0.3% polyethyleneimine, 2 h) Multiscreen Harvest plates using a Tomtec Harvester, washed 9 times with 500 ⁇ L of cold 50 mM Tris-HCl (pH 7.4), and than plates are air-dried in a fumehood for 30 minutes. A bottom seal is applied to the plates prior to the addition of 25 ⁇ L of MicroScint-0 to each well. Plates are than sealed with TopSeal-A and counted for 30 sec per well on a TopCount Microplate Scintillation and Luminescence Counter (PerkinElmer) where results are expressed as counts per minute (cpm).
• TopSeal-A TopCount Microplate Scintillation and Luminescence Counter
• K i values were calculated using a K d value of 0.16 nM for [ 125 I]-motilin (previously determined during membrane characterization).
• D max 1 - test concentration with maximal displacement - non - specific binding total binding - non - specific binding x 100 where total and non-specific binding represent the cpm obtained in the absence or presence of 1 ⁇ M motilin, respectively.
• Binding activity at the motilin receptor for representative compounds of the present invention is shown in Table 3. Compound structures for Table 3 are presented with the various groups as defined for the general structure of formula I. Table 3: Binding Activity at the Human Motilin Receptor for Representative Compounds of the Invention Compound No. K i (nM) Compound No.
• Compounds were provided as dry films at a quantity of approximately 1.2 ⁇ mol in pre-formatted 96-well plates. Compounds were dissolved in 100% DMSO at a concentration of 10 mM and stored at -20°C until further use. Daughter plates were prepared at a concentration of 500 ⁇ M in 30% DMSO with 0.1% BSA and stored at -20°C until testing. On the test day, compounds were allowed to thaw at room temperature and than diluted in assay buffer according to the desired test concentrations. Under these conditions, the maximum final DMSO concentration in the assay was 0.6%.
• Cells are collected from culture plates with Ca 2+ and Mg 2+ -free phosphate buffered saline (PBS) supplemented with 5 mM EDTA, pelleted for 2 minutes at 1000 x g, resuspended in assay buffer (see above) at a density of 5 x 10 6 cells/mL and incubated overnight in the presence of 5 ⁇ M coelenterazine. After loading, cells were diluted with assay buffer to a concentration of 5 x 10 5 cells/mL.
• PBS Ca 2+ and Mg 2+ -free phosphate buffered saline
• assay buffer see above
• test compound or motilin reference agonist
• 50 ⁇ L of the cell suspension was mixed with 50 ⁇ L of the appropriate concentration of test compound or motilin (reference agonist) in 96-well plates (duplicate samples).
• the emission of light resulting from receptor activation was recorded using the Functional Drug Screening System 6000 'FDSS 6000' (Hamamatsu Photonics K.K., Japan).
• an approximate EC 80 concentration of motilin i.e. 0.5 nM; 100 ⁇ L was injected onto the cell suspension containing the test compounds (duplicate samples) 15-30 minutes after the end of agonist testing and the consequent emission of light resulting from receptor activation was measured as described in the paragraph above.
• E the measured RLU value at a given agonist concentration (C)
• E max the maximal response
• EC 50 the concentration producing 50% stimulation
• n is the slope index.
• results for agonist testing results for each concentration of test compound were expressed as percent activation relative to the signal induced by motilin at a concentration equal to the EC 80 (i.e. 0.5 nM). For antagonist testing, results for each concentration of test compound were expressed as percent inhibition relative to the signal induced by motilin at a concentration equal to the EC 80
• ExpresSProfile a broad target screen offered commercially by CEREP (Poitiers, France).
• CEREP CEREP
• a single-point (10 ⁇ M) binding assay is performed across 50 individual receptors from four distinct target classes: non-peptide G-Protein Coupled Receptors (GPCRs), peptide GPCRs, ion channels and amine transporters.
• GPCRs non-peptide G-Protein Coupled Receptors
• peptide GPCRs peptide GPCRs
• ion channels amine transporters
• Binding activity at the motilin receptor for representative compounds of the present invention is shown in Table 4.
• Compound structures for Table 4 are presented in Table 3 with the various groups as defined for the general structure of formula I.
• Table 4 Functional Activity at the Motilin Receptor for Representative Compounds of the Invention Compound No. IC 50 (nM) a Compound No.
• IC 50 (nM) a 502 B 543 C 503 C 544 A 504 C 545 A 505 B 546 A 511 C 547 B 512 C 548 B 513 C 550 A 515 B 552 A 516 C 555 A 529 B 557 B 530 C 563 B 532 C 564 A 533 B 565 B 534 B 568 B 535 B 569 B 536 C 570 A 537 A 571 B 538 A 572 A 539 A 573 B 540 A 574 D 541 B 585 B 542 B 586 C a Functional activity determined using standard method, IC 50 values are expressed as follows: A ⁇ 1 nM, B ⁇ 10 nM, C ⁇ 100 nM, D ⁇ 1000 nM
• CHO membranes were immobilized into 96-well FlashPlate microplates.
• Test compound, GTP ⁇ S, motilin and [ 35 S]-GTP ⁇ S were combined in each well according to the Assay Volumes described above.
• the reaction is initiated by addition of 100 mL of [ 35 S]-GTP ⁇ S to each well. Each plate is sealed (TopSeal-A) and incubated in the dark at room temperature for 150 min. Then, plates are counted for 30 seconds per well on the TopCount NXT.
• Top and Bottom correspond to the top and bottom values of the dose-response curve calculated by GraphPad Prism.
• Duodenal segments were vertically suspended in organ chambers of 10 mL filled with Krebs buffer and connected to an isotonic force transducer, with a preload of 1 g. After a stabilization period, the muscle strips were challenged with 10 -4 M acetylcholine and washed. This was repeated until a stable maximal contraction was obtained (2-3 times), with an interval of at least 20 minutes.
• test compounds were added to the bath. After a 15 minute incubation, a dose response to motilin was recorded by adding logarithmically increasing concentrations of motilin to the bath (final concentration 10 -9 to 10 -6 M). A blank experiment (no test compound present) was also performed. At the end of the dose response curve, a supramaximal dose of acetylcholine (10 -4 M) was given and this response was used as a reference (100% contraction).
• Rat models have been utilized for evaluation of compounds in chemotherapy-induced diarrhea (CID).
• CID chemotherapy-induced diarrhea
• mice have been used to test agents for amelioration of gastrointestinal toxicity of chemotherapeutic agents.
• Boushey RP, Yusta B, Drucker DJ. Cancer Res 2001, 61, 687-93 Zhao, J.; Huang, L.; Belmar, N.; Buelow, R.; Fong, T. Clin. Canc. Res. 2004, 10, 2851-2859 .
• dogs are known to suffer from diarrhea during treatment with common chemotherapeutic agents.
• rats and mice do not possess a functional motilin receptor and would not be appropriate animal models for a motilin antagonist, but could be used to differentiate effects at the motilin receptor from non-specific effects.
• the dog is an appropriate model species as it possesses a functional motilin receptor and has been used previously for studies involving motilin modulation.
• Other species appropriate for such an evaluation include hamster, pig, rabbit, shrew, guinea pig and opossum.
• methods analogous to those described for rats and mice could be adapted to canine or other models to test the compounds of the invention.
• the effect of treatment with compounds of the invention on the frequency and severity of incidences of diarrhea in a dog or other animal model can be used as a measure of the effectiveness of the compounds of the invention.
• the objective of this study was to determine the efficacy of representative macrocyclic compounds of the present invention in chemotherapy-induced diarrhea (CID) following intravenous administration to the male beagle dog ( Canis familiaris ) for up to 32 days. Comparison with existing agents utilized for this condition, octreotide and loperamide, was an additional objective.
• CID chemotherapy-induced diarrhea
• TA1 irinotecan solution or irinotecan hydrochloride
• TA1 irinotecan solution or irinotecan hydrochloride
• Cycle 1 irinotecan solution was administered at a dose level of 4 mg/kg/day by one hour infusion via implanted catheter for 5 consecutive days followed by a 9-day rest period.
• irinotecan solution was administered by one hour infusion via implanted catheter for four consecutive days at a dose level of 4 mg/kg/day and by bolus injection (over approximately one minute) at a dose level of 8 mg/kg/day via implanted catheter for the fifth day followed by a 5-day rest period; for Cycle 3, one spare animal was added to Group 1.
• Irinotecan formulation was prepared with irinotecan hydrochloride powder and administered to the four animals by bolus injection via implanted catheter at a dose level of 4 mg/kg/day for five consecutive days. These four animals were observed for 9 days and subjected to necropsy following an overnight period without food.
• irinotecan formulation was prepared with irinotecan hydrochloride powder and administered by bolus injection via implanted catheter up to two treatment cycles for a 28-day study period. In each treatment cycle, animals were treated with a single daily dose at 6 mg/kg for 5 consecutive days followed by a 9-day rest period without treatment. Before commencing treatment with irinotecan (Group 4) or upon onset of irinotecan-induced diarrhea (Group 2, 3, 6 and 7), these animals were treated with TA2 (compound 552) (Group 2 to 4), octreotide (Group 6) or loperamide (Group 7) as detailed below.
• b TA2 (compound 552) was administered by infusion to all the animals in the group via an implanted catheter over 45 minutes.
• the treatment with compound 552 started on the day of onset of diarrhea, if diarrhea was observed in any of the three animals from the group in the morning or on the following day if diarrhea was observed in the afternoon. Treatment with compound 552 then continued throughout the remainder of the 28-day study period.
• the daily dosage i.e.
• 5.0 and 15.0 mg/kg/day for Groups 2 and 3 respectively was divided into two equal doses (ie 2.5 and 7.5 mg/kg/occasion for Groups 2 and 3 respectively) and infused at least 6 hours apart on each day.
• the animals received 0.9% Sodium Chloride for Injection USP at a rate of 2.5 mL/hour between each infusion of compound 552.
• c TA2 (compound 552) was administered to all three animals in the group following the same procedures for Groups 2 and 3, starting four days before the start of TA 1 (irinotecan) treatment and throughout the 28-day study period.
• the daily dose (5.0 mg/kg/day) was divided into two equal doses (2.5 mg/kg/occasion) and infused at least 6 hours apart on each day.
• the animals in the Vehicle Control group (5% dextrose for injection, USP) were treated with the vehicles for irinotecan and compound 552.
• the vehicle for irinotecan was administered by a slow bolus injection (approximately over one minute) via infusion line for up to two treatment cycles. In each treatment cycle, animals were treated with single daily doses for 5 consecutive days followed by a 9-day rest period.
• the vehicle for compound 552 was administered by following the dosing procedures for compound 552 administration or 23 days (from Day 6 to Day 28.
• mice in the group were dosed with octreotide by subcutaneous injection on the day of onset of irinotecan-induced diarrhea, if diarrhea was observed in any of the three animals from the group in the morning or on the following day if diarrhea was observed in the afternoon.
• the treatment was maintained throughout the remainder of the 28-day study period.
• the daily dosage (0.021 mg/kg/day) was divided into three equal doses (0.007 mg/kg/occasion) and administered by subcutaneous injection early in the morning, noon and late in the afternoon, respectively. Each administration was separated by at least 4 hours ( ⁇ 30 minutes).
• test compounds, controls and comparators were prepared at appropriate intervals. All formulations intended for administration to the test animals were stored refrigerated at 2-8°C. Irinotecan was obtained as a sterile solution for intravenous injection containing irinotecan hydrochloride trihydrate at a concentration of 20 mg/mL, ready to use. The irinotecan formulation for dosing the animals (0.5 mg/mL) in Group 1 (Cycles 1 and 2 only) was achieved by dilution of 20 mg/mL formulation with 5% dextrose for injection, USP. The dosing formulation was stored refrigerated (2 to 8°C), protected from light and used within 48 hours.
• irinotecan For the remaining treatment with irinotecan (i.e. Cycle 3 for Group 1; Groups 2 to 4; Groups 6 and 7), a stock irinotecan formulation was prepared from irinotecan solid (irinotecan hydrochloride) and used for preparation of a dose formulation as described below:
• Octreotide was provided as a sterile lyophilisate and reconstituted with sterile water for injection to yield a stock solution (10 mg/mL).
• the stock solution was stored refrigerated (2 to 8°C). On each day of dosing, the stock solution is diluted with sterile water for injection to a final concentration of 0.1 mg/mL.
• Compound 552 was formulated as a solution with 10% hydroxypropyl-13-cyclodextrin (97%) in water.
• representative samples 1.0 mL in duplicate, except for octreotide, where only 0.1 mL each will be collected) were collected at appropriate intervals during the study.
• a total of 21 males and 2 spare males were obtained from a commercial supplier (for example Marshall BioResources, North Rose, New York) with ages 5 to 7 months old at onset of treatment and weights 6-9 kg at onset of treatment.
• the animals were acclimated between receipt of the animals and the start of treatment to accustom the dogs to the laboratory environment prior to the start of the study approximately 3 weeks for Group 1, 5 weeks for Group 2 to 4, and 3 weeks for Group 5 to 7.
• Each dog was housed in a stainless steel cage equipped with an automatic watering system supplemented by water bottles as appropriate. Each cage was clearly labeled with a color-coded cage card indicating the study, group and animal numbers, sex and dose level.
• Each animal was uniquely identified by the supplier by means of a tattoo on the ventral aspect of one pinna.
• the animal room environment was controlled (targeted ranges: temperature 21 ⁇ 3°C, relative humidity 50 ⁇ 20%, 12 hours light, 12 hours dark, 10-15 air changes per hour) except during designated procedures. Temperature and relative humidity were monitored continuously and recorded 4 times daily.
• a standard certified commercial dog chow (for example 400 g of Teklad Certified 25% Lab Dog Diet #8727C) was available to each dog once daily during a minimum two-hour feeding period but up to a maximum of 4 hours. During acclimation, the animals were slowly acclimated to the two-hour feeding regimen. Concentrations of the constituents of the diet and contaminants (e.g., heavy metals, aflatoxin, organophosphates, pesticides and chlorinated hydrocarbons) were controlled and routinely measured by the manufacturers. Municipal tap water (which has been purified by reverse osmosis, ultraviolet light and further filtered with a 0.2 ⁇ m filter) was provided to the animals ad libitum except during designated procedures.
• Municipal tap water (which has been purified by reverse osmosis, ultraviolet light and further filtered with a 0.2 ⁇ m filter) was provided to the animals ad libitum except during designated procedures.
• the dogs were prepared for surgery approximately 2-3 weeks before starting treatment. These animals were food deprived overnight prior to the surgical procedure and water was removed on the morning of surgery to insert an intravenous catheter.
• the dogs were injected intramuscularly with a pre-anesthetic cocktail (e.g. butorphanol, acepromazine and glycopyrrolate) for preparation of the surgical sites.
• a pre-anesthetic cocktail e.g. butorphanol, acepromazine and glycopyrrolate
• animals were anesthetized by isoflurane inhalation.
• the intravenous catheter consisted of a length of medical-grade tubing inserted into the femoral vein and advanced into the vena cava.
• the catheter was exteriorized at the nape of the neck using a trocar which created a subcutaneous tunnel from the inguinal area to the dorso-cervical area. During the recovery from anesthesia, each animal was dressed in a clean jacket.
• the catheter was attached to a swivel device, via a jacket and tether system, and connected to a syringe by another section of medical-grade tubing.
• the pumps and reservoirs were contained in a specially designed box positioned on the exterior of the cage.
• antibiotic for example benzanthine penicillin G and procaine penicillin G
• a topical antiseptic was applied to the surgical site once daily, as needed.
• the syringes/bags containing the saline solution were changed with appropriate frequency during the pretreatment period.
• cycle 1 For the animals in Group 1, treatment with test article No. 1 (irinotecan) consisted of three (3) cycles: Cycle 1, irinotecan was administered at a dose level of 4 mg/kg/day by one hour infusion via implanted catheter for 5 consecutive days followed by a 9-day rest period. Cycle 2, irinotecan was administered by one hour infusion via implanted catheter for four consecutive days at a dose level of 4 mg/kg/day and by bolus injection (approximately over one minute) at a dose level of 8 mg/kg/day via implanted catheter for the fifth day followed by a 5-day rest period; Cycle 3, one spare animal was added to Group 1.
• Irinotecan formulation was prepared with irinotecan hydrochloride powder and administered to the four animals by bolus injection via implanted catheter at a dose level of 4 mg/kg/day for five consecutive days.
• irinotecan formulation that was prepared with irinotecan hydrochloride powder was administered by bolus injection via implanted catheter up to two treatment cycles for a 28-day study period. In each treatment cycle, animals were treated with a single daily dose at 6 mg/kg for 5 consecutive days followed by a 9-day rest period without treatment.
• the control/vehicle article for irinotecan was administered to all dogs in Group 5 by following the same dosing procedures for irinotecan administration.
• the dose volume was 8 mL/kg for Cycle 1 and the first 4 days of Cycle 2 for Group 1, 2 mL/kg for the last day of Cycle 2 for Group 1, and 3 mL/kg for Groups 2 to 4, 6 and 7.
• the actual volume infused to each dog was calculated and adjusted based on the most recent practical body weight of each animal.
• the test article No. 2 (compound 552) was intravenously infused via an implanted catheter over 45 minutes to all the animals in Group 4 four days before the start of TA1 (irinotecan) treatment and throughout the 28-day study period.
• TA2 (compound 552) was administered to all the animals in Groups 2 and 3 on the same day of onset of irinotecan-induced diarrhea, if diarrhea was observed in any one of the three animals from the group in the morning, or on the following day of diarrhea onset if diarrhea was observed in anyone of the three animals in the group in the afternoon, and throughout the remainder of the 28-day study period.
• the daily dosage i.e.
• 5.0, 15.0 and 5.0 mg/kg/day for Groups 2 to 4 respectively was administered by two equal doses (i.e. 2.5, 7.5 and 2.5 mg/kg/occasion for Groups 2 to 4 respectively) which were separate by at least 6 h.
• Each dose was infused at a dose volume of 10 mL/kg/h.
• the vehicle for compound 552 was administered to all dogs in Group 5 by following the same dosing procedures and regimen for compound 552 administration for 23 days (from Day 6 to Day 28).
• the actual volume infused was calculated and adjusted based on the most recent practical body weight of each animal.
• the syringes/bags were changed at appropriate intervals as necessary and the weights recorded prior to the start and at the end of the infusion.
• Octreotide was administered by subcutaneous injection to all animals in Group 6 starting on the same day of diarrhea onset, if diarrhea was observed in any one of the three animals from the group in the morning, or on the following day of diarrhea onset, if diarrhea was observed in any one of the three animals in the goup in the afternoon.
• the treatment was maintained throughout the remainder of the 28-day study period.
• the daily dosage (0.021 mg/kg/day) was divided into three equal doses (0.007 mg/kg/occasion) and administered by subcutaneous injection approximately early in the morning, noon and late in the afternoon, respectively. Each administration was separated by at least 4 hours ( ⁇ 30 minutes).
• the dose volume for each administration was 0.07 mL/kg. The actual dose volume was calculated and adjusted based on the most recent practical body weight of each animal.
• Loperamide was administered by oral gavage to all animals in Group 7 starting on the same day of diarrhea onset, if diarrhea was observed in any one of the three animals of the group in the morning, or on the following day of diarrhea onset, if diarrhea was observed in anyone of the three animals in the group in the afternoon.
• the treatment was maintained throughout the remainder of the 28-day study period.
• the daily dosage (0.18 mg/kg/day) was divided into three equal doses (0.06 mg/kg/occasion) and administered by oral gavage approximately early in the morning, noon and late in the afternoon, respectively. Each administration was separated at least 4 h ( ⁇ 30 min).
• the dose volume for each administration was 0.6 mL/kg. The actual dose volume was calculated and adjusted based on the most recent practical body weight of each animal.
• the infusion line in each animal was maintained by infusion of 0.9% Sodium Chloride for Injection USP at a rate of 2.5 mL/h between each treatment of irinotecan, compound 552 or vehicles.
• Cage-side clinical signs (ill health, behavioral changes, stool changes etc.) were recorded once daily during the acclimation period except on detailed clinical examination days. Cage-side clinical signs were recorded twice a day (am and pm) during the treatment/observation period except on the days of a detailed clinical examination where they were recorded once (PM). A detailed clinical examination of each dog was performed once pretreatment, weekly during the treatment/observation periods and before necropsy. Particular attention was paid to the surgical sites.
• Body weights were recorded for all animals once prior to group assignment, and approximately one week prior to initiation of treatment. Body weights were recorded for all animals up to 1 day prior to dosing and twice weekly thereafter during the treatment/observation periods, as well as terminally prior to necropsy (fasted). Food consumption was measured daily during the week prior to treatment and throughout the treatment/observation periods.
• a series of blood samples (approximately 2.0 mL each) were removed from each dog in Groups 2 and 3 after the first (am) infusion on the first day of treatment with compound 552 and after the second (pm) infusion on the last day of treatment with compound 552. (Note: the second (pm) infusion on the first day of treatment with compound 552 was performed after completion of the last blood sampling).
• each dog was bled by venipuncture and the samples collected into tubes containing the anticoagulant, K 2 EDTA. Tubes were placed on wet ice pending processing. On each occasion, samples were collected prior to the start of infusion, 5, 15, 30, 45, 50, 65, 95, 135 minutes, 3 and 6 hours after the start of infusion of compound 552. Following collection, the samples were centrifuged at approximately 1500xg, approximately 4°C, for at least 10 minutes and the resulting plasma recovered and dispensed into two aliquots and stored frozen (at approximately -80°C) pending shipment.
• the samples were centrifuged at approximately 1500xg, approximately 4°C, for at least 10 minutes and the resulting plasma recovered and dispensed into two aliquots and stored frozen (at approximately -80°C) pending shipment.
• the data was analyzed for homogeneity of variance using Levene Median and for normality using Kolmogorov-Smimov tests.
• Homogeneous data was analyzed using the Analysis of Variance and the significance of intergroup differences was analyzed using Dunnett's test.
• Heterogeneous data was analyzed using Kruskal-Wallis test and the significance of intergroup differences between the control and treated groups assessed using Dunn's test. A significance level of p ⁇ 0.05 was reported.
• compound 552 a potent and selective motilin antagonist, demonstrated superior efficacy in the treatment of irinotecan-induced CID in dogs versus the current standard of care.
• the compound proved to be more effective, with a quicker onset and longer duration of action as well.
• Plasma concentrations of compound 552 in male beagle dogs receiving low and high intravenous doses of compound 552 as part of the above experiment were determined.
• Plasma samples were prepared for analysis by solid phase extraction and analyzed by LC-MS/MS.
• the average C max observed at 30 min after the start of the 45-min infusion was about 1.5 and 1.8 ⁇ g/mL on the first and last day of treatment with compound 552, respectively, for the animals receiving 2.5 mg/kg.
• the average C max observed for the high dose group administered with 7.5 mg/kg occurred between 15 and 45 min after the start of the 45-min infusion and was about 3.4 and 5.0 ⁇ g/mL on the first and last day of treatment with compound 552, respectively.
• the average AUC 0-t was similar on the first (78,233 ng.min/mL) and last day (83,047 ng.min/mL) of dosing for the low dose group (2.5 mg/kg/occasion) and was proportionally higher on the first day (246,073 ng.min/mL) of dosing for the high dose group (7.5 mg/kg/occasion).
• the average AUC 0-t was higher (380,758 ng.min/mL) on the last day of dosing for the high dose group, however the higher average value-resulted from one animal exhibiting particularly high AUC 0-t .
• the plasma concentration data demonstrated dose-related exposure for compound 552 in beagle dogs during the CID efficacy study.
• Cytochrome P450 enzymes are implicated in the phase I metabolism of drugs. The majority of drug-drug interactions are metabolism-based and, moreover, these interactions typically involve inhibition of cytochrome P450s. Six CYP450 enzymes (CYPIA2, CYP2C8, CYP2C9, CYP2C19, CYP2D6 and CYP3A4) appear to be commonly responsible for the metabolism of most drugs and the associated drug-drug interactions. Assays to determine the binding of compounds of the invention to the various metabolically important isoforms of cytochrome P450 metabolizing enzymes are commercially available, for example NoAb BioDiscoveries (Mississaugua, ON, Canada) and Absorption Systems (Exton, PA, USA).
• the pharmacokinetic and pharmacodynamic properties of drugs are largely a function of the reversible binding of drugs to plasma or serum proteins such as albumin and ⁇ 1 -acid glycoprotein.
• plasma or serum proteins such as albumin and ⁇ 1 -acid glycoprotein.
• drugs with low plasma protein binding generally have large volumes of distribution and rapid clearance since only unbound drug is available for glomerular filtration and, in some cases, hepatic clearance.
• the ideal range for plasma protein binding is in the range of 87-98% for most drug products.
• Protein binding studies were performed using human plasma. Briefly, 96-well microplates were used to incubate various concentrations of the test article for 60 min at 37°C. Bound and unbound fractions are separated by equilibrium dialysis, where the concentration remaining in the unbound fraction is quantified by LC-MS or LC-MS-MS analysis. Drugs with known plasma protein binding values such as quinine ( ⁇ 35%), warfarin ( ⁇ 98%) and naproxen ( ⁇ 99.7%) were used as reference controls.
• Table 6 Results for representative compounds of the invention are summarized in Table 6.
• Table 8 Inhibition of CYP P450 Isozymes by Representative Compounds of the Invention Compound IC 50 ( ⁇ M) a 3A4 3A4 1A2 2A6 2B6 2C8 2C9 2C19 2D6 2E1 (BQ) (BFC) 551 10.4 2.37 n/a n/a n/a 26.9 37.0 30.1 >100 553 3.48 0.0192 n/a n/a n/a >100 44.5 n/a 80.8 >100 a n/a indicates IC 50 value much above 100 ⁇ M
• the Caco-2 cell line derived from a human colorectal carcinoma, has become an established in vitro model for the prediction of drug absorption across the human intestine.
• Assays to determine the permeability of compounds of the invention utilizing Caco-2 cells are commercially available, for example NoAb BioDiscoveries (Mississaugua, ON, Canada) and Absorption Systems (Exton, PA, USA).
• PAMPA parallel artificial membrane permeability assays
• Permeability across the Caco-2 cell layer was determined by growing the cells on a membrane placed between two (donor and acceptor) chambers. Drug candidates are typically added to the apical (A) side of the cell layer and their appearance in the basolateral (B) side is measured over incubation time. Permeability in this direction represents intestinal absorption. Permeability may also be determined from the basolateral to the apical side of the Caco-2 cell. A higher apical to basolateral P app , compared to the basolateral to apical P app , is indicative of carrier-mediated transport. P-gp mediated transport is suggested when a higher basolateral to apical P app is observed relative to the apical to basolateral P app .
• Permeability (10 ⁇ M) for compounds of the invention in the apical to basolateral and basolateral to apical direction were tested in duplicate. Samples will be collected from the donor and acceptor chambers at the beginning (0 min) and following 60 min of incubation at 37°C and stored frozen at -70°C until bioanalysis. Samples for each test compound generated from the Caco-2 permeability assay were further analyzed by LC-MS-MS. The permeability of [ 3 H]-mannitol and [ 3 H]-propranolol were determined in parallel as controls.
• the pharmacokinetic behavior of compound of the invention can be ascertained by methods well known to those skilled in the art. ( Wilkinson, G. R. "Pharmacokinetics: The Dynamics of Drug Absorption, Distribution, and Elimination” in Goodman & Gilman's The Pharmacological Basis of Therapeutics, Tenth Edition, Hardman, J.G.; Limbird, L.E., Eds., McGraw Hill, Columbus, OH, 2001, Chapter 1 .) The following method was used to investigate the pharmacokinetic parameters (elimination half-life, total plasma clearance, etc.) for intravenous, subcutaneous and oral administration of compounds of the present invention.
• Rats male, Sprague-Dawley ( ⁇ 250 g)
• Rats/Treatment Group 6 (2 subsets of 3 rats each, alternate bleeds)
• test compound was sent in solution in a formulation (such as with cyclodextrin) appropriate for dosing. It will be appreciated by one skilled in the art that appropriate modifications to this protocol can be made as required to adequately test the properties of the compound under analysis.
• 0.7 mL of blood were collected from each animal. It is expected that this volume of blood will yield a sample of at least 0.3 mL of plasma.
• EDTA was used as an anti-coagulant for whole blood collection. Whole blood samples were chilled and immediately processed by centrifugation to obtain plasma.
• Plasma samples were stored frozen (-70°C) until analysis.
• Analytical detection of parent compound in plasma samples performed by LC-MS after an appropriate preparation protocol: extraction using solid phase extraction (SPE) cartridges (Oasis MCX, Oasis HLB) or liquid-liquid extraction.
• SPE solid phase extraction
• na not applicable c Done in the presence of 10% beta-cyclodextrin d Done without cyclodextrin
• motilin stimulates the contractile activity in smooth muscle strips isolated from the rabbit gastric antrum ( Van Assche, G.; Depoortere, I.; Thijs, T.; Janssens, J.J.; Peeters, T.L. Concentration-dependent stimulation of cholinergic motor nerves or smooth muscle by [Nle13]-motilin in the isolated rabbit gastric antrum. Eur. J. Pharmacol. 1997, 337, 267-274 .) or from the circular muscle of-the duodenum as described in Method 3-D above.
• Motilin (threshold concentration of 10 -8 M) enhances contractions induced by electrical field stimulation (4 Hz) by a post-ganglionic interaction with the cholinergic neurotransmission. At higher concentrations, motilin causes a tonic contraction interacting directly with the antral smooth muscle. Analogously, the effects of motilin and [Nle 13 ]-motilin (Calbiochem), as selective agonists of the motilin receptor, on smooth muscle strips isolated from the gastric antrum, the circular muscle of the duodenum and the colon of the shrew were investigated.
• test compounds were tested on different regions of the GI tract of the shrew (gastric antrum, ileum and colon) and to evaluate the antagonist affinity of test compounds in this model system.
• the ability of varying concentrations of test compound to antagonize the motilin or [Nle 13 ]-motilin-induced contractions was measured in organ baths as described above.
• Compound 552 (0.01 ⁇ M-10 ⁇ M) caused a dose-dependent inhibition of the contractile response to 0.3 ⁇ M motilin or to 0.3 ⁇ M [Nle 13 ]-motilin.
• data are mean ⁇ SEM and the number of animals was as indicated for each treatment.
• Compound 552 did not cause a significant change in basal contractile activity (data not shown).
• Animal models can be used to investigate the effect of treatment with representative compounds of the present invention on amelioration of the symptoms or prevention of the development of diarrhea induced in different manners, in particular by stress or by PGE 2 .
• rats and mice do not express motilin receptors
• the musk shrew ( Suncus murinus ) is one appropriate animal model expressing the motilin receptor and suitable for this work.
• opossum, rabbit or the pig are also suitable animal models for this type of evaluation.
• Food and water was provided ad libitum in accordance with the species requirements: Food: 3 part Purina Cat Chow and 1 part Mink Chow (for example Wisconsin "Mink Complete Pellets-Grow-Fur”: crude protein ⁇ 34%, crude fat >20%, crude fiber >4%).
• the food was placed in a small plastic dish within the cage.
• Water Slightly acidic (pH 5.5) distilled water, to reduce contamination of tap water supply.
• the cages and water bottles were changed weekly. All animals were acclimated to the animal facility for at least 1 week prior to initiating the study.
• the shrews were placed in individual restraint cages for 1 h at room temperature. Fecal output (number of pellets) was measured during 1 h of immobilization and within 1 h after the restraint stress. The consistency was evaluated using a 3 level scoring system: 0, normal; 1, soft; 2 unformed. ( Saito, T.; Mizutani, F.; Iwanaga, Y.; Morikawa, K.; Kato, H. Laxative and anti-diarrheal activity of polycarbophil in mice and rats. Jpn. J.
• test compound 0.1, 1 and 10 mg/kg s.c. or other appropriate doses and mode of administration
• vehicle Animals were placed in individual restraint cages for 1 h at room temperature and fecal output was measured as described above. The animals had free access to food and water prior to the experiment.
• test compound 0.1, 1 and 10 mg/kg s.c. or other appropriate doses and mode of administration
• vehicle 0.1, 1 and 10 mg/kg s.c. or other appropriate doses and mode of administration
• Diarrhea was induced by administration of PGE 2 (0.3 mg/kg, i.p.) and changes in stool consistency were evaluated as described above.
• the animals were acclimated with free access to food and water.
• the macrocyclic compounds of the present invention or pharmacologically acceptable salts thereof according to the invention may be formulated into pharmaceutical compositions of various dosage forms.
• one or more compounds, including optical isomers, enantiomers, diastereomers, racemates or stereochemical mixtures thereof, or pharmaceutically acceptable salts thereof as the active ingredient is intimately mixed with appropriate carriers and additives according to techniques known to those skilled in the art of pharmaceutical formulations.
• a pharmaceutically acceptable salt refers to a salt form of the compounds of the present invention in order to permit their use or formulation as pharmaceuticals and which retains the biological effectiveness of the free acids and bases of the specified compound and that is not biologically or otherwise undesirable. Examples of such salts are described in Handbook of Pharmaceutical Salts: Properties, Selection, and Use, Wermuth, C.G. and Stahl, P.H. (eds.), Wiley-Verlag Helvetica Acta, Zurich, 2002 [ISBN 3-906390-26-8 ].
• salts include alkali metal salts and addition salts of free acids and bases:
• pharmaceutically acceptable salts include sulfates, pyrosulfates, bisulfates, sulfites, bisulfites, phosphates, monohydrogenphosphates, dihydrogenphosphates, metaphosphates, pyrophosphates, chlorides, bromides, iodides, acetates, propionates, decanoates, caprylates, acrylates, formates, isobutyrates, caproates ; heptanoates, propiolates, oxalates, malonates, succinates, suberates, sebacates, fumarates, maleates, butyne-1,4-dioates, hexyne-1,6-dioates, benzoates, chlorobenzoates, methylbenzoates, dinitrobenzoates, hydroxybenzoates, methoxybenzoates, phthalates,
• a desired salt may be prepared by any suitable method known to those skilled in the art, including treatment of the free base with an inorganic acid, such as, without limitation, hydrochloric acid, hydrobromic acid, hydroiodic, carbonic acid, sulfuric acid, nitric acid, phosphoric acid, and the like, or with an organic acid, including, without limitation, formic acid, acetic acid, propionic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, stearic acid, ascorbic acid, glycolic acid, salicylic acid, pyranosidyl acid, such as glucuronic acid or galacturonic acid, alpha-hydroxy acid, such as citric acid or tartaric acid, amino acid, such as aspartic acid or glutamic acid, aromatic acid, such as benzoic acid or cinnamic acid, sulfonic acid, such as p-tol
• an inorganic acid such
• an inventive compound is an acid
• a desired salt may be prepared by any suitable method known to the art, including treatment of the free acid with an inorganic or organic base, such as an amine (primary, secondary, or tertiary); an alkali metal or alkaline earth metal hydroxide; or the like.
• an inorganic or organic base such as an amine (primary, secondary, or tertiary); an alkali metal or alkaline earth metal hydroxide; or the like.
• suitable salts include organic salts derived from amino acids such as glycine, lysine and arginine; ammonia; primary, secondary, and tertiary amines such as ethylenediamine, N,N'-dibenzylethylenediamine, diethanolamine, choline, and procaine, and cyclic amines, such as piperidine, morpholine, and piperazine; as well as inorganic salts derived from sodium, calcium, potassium, magnesium, manganese, iron, copper, zinc, aluminum, and lithium.
• compositions for oral administration may be, for example, solid preparations such as tablets, sugar-coated tablets, hard capsules, soft capsules, granules, powders and the like, with suitable carriers and additives being starches, sugars, binders, diluents, granulating agents, lubricants, disintegrating agents and the like. Because of their ease of use and higher patient compliance, tablets and capsules represent the most advantageous oral dosage forms for many medical conditions.
• compositions for liquid preparations include solutions, emulsions, dispersions, suspensions, syrups, elixirs, and the like with suitable carriers and additives being water, alcohols, oils, glycols, preservatives, flavoring agents, coloring agents, suspending agents, and the like.
• suitable carriers and additives being water, alcohols, oils, glycols, preservatives, flavoring agents, coloring agents, suspending agents, and the like.
• Typical preparations for parenteral administration comprise the active ingredient with a carrier such as sterile water or parenterally acceptable oil including polyethylene glycol, polyvinyl pyrrolidone, lecithin, arachis oil or sesame oil, with other additives for aiding solubility or preservation may also be included.
• a carrier such as sterile water or parenterally acceptable oil including polyethylene glycol, polyvinyl pyrrolidone, lecithin, arachis oil or sesame oil, with other additives for aiding solubility or preservation may
• compositions according to embodiments of the present invention include those suitable for oral, rectal, topical, inhalation (e.g., via an aerosol) buccal (e.g., sub-lingual), vaginal, topical (i.e., both skin and mucosal surfaces, including airway surfaces), transdermal administration and parenteral (e.g., subcutaneous, intramuscular, intradermal, intraarticular, intrapleural, intraperitoneal, intrathecal, intracerebral, intracranially, intraarterial, or intravenous), although the most suitable route in any given case will depend on the nature and severity of the condition being treated and on the nature of the particular active agent which is being used.
• compositions for injection will include the active ingredient together with suitable carriers including propylene glycol-alcohol-water, isotonic water, sterile water for injection (USP), emulPhorTM-alcohol-water, cremophor-ELTM or other suitable carriers known to those skilled in the art.
• suitable carriers including propylene glycol-alcohol-water, isotonic water, sterile water for injection (USP), emulPhorTM-alcohol-water, cremophor-ELTM or other suitable carriers known to those skilled in the art.
• carriers may be used alone or in combination with other conventional solubilizing agents such as ethanol, propylene glycol, or other agents known to those skilled in the art.
• the compounds may be used by dissolving or suspending in any conventional diluent.
• the diluents may include, for example, physiological saline, Ringer's solution, an aqueous glucose solution, an aqueous dextrose solution, an alcohol, a fatty acid ester, glycerol, a glycol, an oil derived from plant or animal sources, a paraffin and the like. These preparations may be prepared according to any conventional method known to those skilled in the art.
• compositions for nasal administration may be formulated as aerosols, drops, powders and gels.
• Aerosol formulations typically comprise a solution or fine suspension of the active ingredient in a physiologically acceptable aqueous or non-aqueous solvent.
• Such formulations are typically presented in single or multidose quantities in a sterile form in a sealed container.
• the sealed container can be a cartridge or refill for use with an atomizing device.
• the sealed container may be a unitary dispensing device such as a single use nasal inhaler, pump atomizer or an aerosol dispenser fitted with a metering valve set to deliver a therapeutically effective amount, which is intended for disposal once the contents have been completely used.
• the dosage form comprises an aerosol dispenser, it will contain a propellant such as a compressed gas, air as an example, or an organic propellant including a fluorochlorohydrocarbon or fluorohydrocarbon.
• compositions suitable for buccal or sublingual administration include tablets, lozenges and pastilles, wherein the active ingredient is formulated with a carrier such as sugar and acacia, tragacanth or gelatin and glycerin.
• a carrier such as sugar and acacia, tragacanth or gelatin and glycerin.
• compositions for rectal administration include suppositories containing a conventional suppository base such as cocoa butter.
• compositions suitable for transdermal administration include ointments, gels and patches.
• compositions known to those skilled in the art can also be applied for percutaneous or subcutaneous administration, such as plasters.
• compositions comprising the active ingredient or ingredients in admixture with components necessary for the formulation of the compositions
• other conventional pharmacologically acceptable additives may be incorporated, for example, excipients, stabilizers, antiseptics, wetting agents, emulsifying agents, lubricants, sweetening agents, coloring agents, flavoring agents, isotonicity agents, buffering agents, antioxidants and the like.
• additives there may be mentioned, for example, starch, sucrose, fructose, dextrose, lactose, glucose, mannitol, sorbitol, precipitated calcium carbonate, crystalline cellulose, carboxymethylcellulose, dextrin, gelatin, acacia, EDTA, magnesium stearate, talc, hydroxypropylmethylcellulose, sodium metabisulfite, and the like.
• the composition is provided in a unit dosage form such as a tablet or capsule.
• kits including one or more containers comprising pharmaceutical dosage units comprising an effective amount of one or more compounds of the present invention.
• the present invention further provides prodrugs comprising the compounds described herein.
• prodrug is intended to mean a compound that is converted under physiological conditions or by solvolysis or metabolically to a specified compound that is pharmaceutically active.
• the "prodrug” can be a compound of the present invention that has been chemically derivatized such that, (i) it retains some, all or none of the bioactivity of its parent drug compound, and (ii) it is metabolized in a subject to yield the parent drug compound.
• the prodrug of the present invention may also be a "partial prodrug" in that the compound has been chemically derivatized such that, (i) it retains some, all or none of the bioactivity of its parent drug compound, and (ii) it is metabolized in a subject to yield a biologically active derivative of the compound.
• Known techniques for derivatizing compounds to provide prodrugs can be employed. Such methods may utilize formation of a hydrolyzable coupling to the compound.
• the present invention further provides that the compounds of the present invention may be administered in combination with a therapeutic agent used to prevent and/or treat metabolic and/or endocrine disorders, gastrointestinal disorders, cardiovascular disorders, obesity and obesity-associated disorders, central nervous system disorders, genetic disorders, hyperproliferative disorders and inflammatory disorders.
• a therapeutic agent used to prevent and/or treat metabolic and/or endocrine disorders, gastrointestinal disorders, cardiovascular disorders, obesity and obesity-associated disorders, central nervous system disorders, genetic disorders, hyperproliferative disorders and inflammatory disorders.
• agents include analgesics (including opioid analgesics), anesthetics, antifungals, antibiotics, antiinflammatories (including nonsteroidal anti-inflammatory agents), anthelmintics, antiemetics, antihistamines, antihypertensives, antipsychotics, antiarthritics, antitussives, antivirals, cardioactive drugs, cathartics, chemotherapeutic agents (such as DNA-interactive agents, antimetabolites, tubulin-interactive agents, hormonal agents, and agents such as asparaginase or hydroxyurea), corticoids (steroids), antidepressants, depressants, diuretics, hypnotics, minerals, nutritional supplements, parasympathomimetics, hormones (such as corticotrophin releasing hormone, adrenocorticotropin, growth hormone releasing hormone, growth hormone, thyrptropin-releasing hormone and thyroid stimulating hormone), sedatives, sulfonamides, stimulants, sympathomimetics, tranquilizer
• Subjects suitable to be treated according to the present invention include, but are not limited to, avian and mammalian subjects, and are preferably mammalian.
• Mammals of the present invention include, but are not limited to, canines, felines, bovines, caprines, equines, ovines, porcines, rodents ( e.g . rats and mice), lagomorphs, primates, humans, and the like, and mammals in utero. Any mammalian subject in need of being treated according to the present invention is suitable.
• Human subjects are preferred. Human subjects of both genders and at any stage of development ( i.e ., neonate, infant, juvenile, adolescent, adult) can be treated according to the present invention.
• Illustrative avians according to the present invention include chickens, ducks, turkeys, geese, quail, pheasant, ratites ( e.g., ostrich) and domesticated birds ( e.g., parrots and canaries), and birds in ovo.
• ratites e.g., ostrich
• domesticated birds e.g., parrots and canaries
• the present invention is primarily concerned with the treatment of human subjects, but the invention can also be carried out on animal subjects, particularly mammalian subjects such as mice, rats, dogs, cats, livestock and horses for veterinary purposes.
• animal subjects particularly mammalian subjects such as mice, rats, dogs, cats, livestock and horses for veterinary purposes.
• the invention can be further carried out for drug screening and drug development purposes.
• the compounds of the present invention or an appropriate pharmaceutical composition thereof may be administered in an effective amount. Since the activity of the compounds and the degree of the therapeutic effect vary, the actual dosage administered will be determined based upon generally recognized factors such as age, condition of the subject, route of delivery and body weight of the subject.
• the dosage can be from about 0.1 to about 100 mg/kg, administered orally 1-4 times per day.
• compounds can be administered by injection at approximately 0.01 - 20 mg/kg per dose, with administration 1-4 times per day. Treatment could continue for weeks, months or longer. Determination of optimal dosages for a particular situation is within the capabilities of those skilled in the art.
• the compounds of formula I of the present invention can be used for the prevention and treatment of a range of gastrointestinal disorders characterized by hypermotility or hypermotilinemia.
• the macrocyclic compounds of the present invention can be used to treat diarrhea, cancer treatment-related diarrhea, chemotherapy-induced diarrhea, radiation enteritis, radiation-induced diarrhea, stress-induced diarrhea, chronic diarrhea, AIDS-related diarrhea, C . difficile associated diarrhea, traveller's diarrhea, diarrhea induced by graph versus host disease and other types of diarrhea.
• the present invention is directed to a method of treating irritable bowel syndrome, inflammatory bowel disease, dyspepsia, functional gastrointestinal disorders, chemotherapy-induced nausea and vomiting (emesis), post-operative nausea and vomiting, Crohn's disease, gastroesophogeal reflux disorders, ulcerative colitis, pancreatitis, infantile hypertrophic pyloric stenosis, carcinoid syndrome, malabsorption syndrome, diabetes mellitus, obesity, postgastroenterectomy syndrome, atrophic colitis or gastritis, gastric stasis, gastrointestinal dumping syndrome, celiac disease, short bowel syndrome, cachexia and eating disorders leading to obesity in humans and other mammals comprising administering a therapeutically effective amount of a compound of formula I.
• treatment is not necessarily meant to imply cure or complete abolition of the disorder or symptoms associated therewith.
• the compounds of the present invention can further be utilized for the preparation of a medicament for the treatment of a range of medical conditions involving gastrointestinal motility disorders.
• Step 1-2 Thiazol-2-yl-carbamic acid benzyl ester (AA1-2).
• 2- aminothiazole AA1-A , 3.0 g, 30.0 mmol
• triethylamine 6.30 mL, 45.0 mmol
• benzyl chloroformate 5 mL, 36 mmol
• the reaction was stirred at RT O/N.
• the reaction mixture was washed with a saturated aqueous solution of NaHCO 3 , then water and concentrated to dryness under reduced pressure. The resulting yellow solid was crystallized from ethanol to afford AA1-2 in 70% yield.
• Step 1-3 Boc-Dap(Z-thiazol-2-yl) (Boc-AA1) This procedure is based on that presented for other substrates in Gautam Panda et al. SynLett 2004, 4, 714-716 .
• a mixture of Boc-Ser-Weinreb amide AA1-1 (3.0 g, 12.0 mmol) and triphenylphosphine (4.75 g, 18 mmol) in anhydrous THF (100 mL) was cooled in an ice bath.
• a solution of DIAD (3.63 g, 18 mmol) in THF (20 mL) was added to this mixture dropwise.
• Step 2-1 Boc-Serine- ⁇ -lactone (AA2-1). This procedure is based on that found in the literature ( Vederas, J.C.; et al. J. Am. Chem. Soc. 1987, 109, 4649-4659 ). Into a dry 250 mL 3-neck flask equipped with a mechanical stirrer under a nitrogen atmosphere was added triphenylphosphine (4.5 g, 17.1 mmol, 1.1 eq), followed by 100 mL of an anhydrous THF:CH 3 CN (1:9) mixture.
• triphenylphosphine 4.5 g, 17.1 mmol, 1.1 eq
• Step 2-2 1-Trimethylsilylimidazole (AA2-2).
• HMDS 11.46 mL, 0.055 mol, 1.5 eq
• imidazole 5.0 g, 0.074 mol, 2 eq
• the reaction mixture heated to reflux for 3 h.
• the reaction mixture was then concentrated to dryness under reduced pressure. Distillation of the residue gave AA2-2 as a colorless oil in 85%.
• this product should be stored at 0°C under argon.
• Step 2-3 Boc-imidazol-1-yl-Ala (AA2). Similar to the method described in Vederas, J.C.; et al. J. Am. Chem. Soc. 1985, 107, 7105-7109 , trimethylsilylimidazole AA2-2 (0.488 g, 3.50 mmol, 1.3 eq) in dry CH 3 CN (10 mL) was treated dropwise over 5 min with AA2-1 (0.5 g, 2.67 mmol, 1 eq) in dry CH 3 CN (10 mL) under argon, then the mixture stirred for 28 h.
• Z-Dap(Me)-OH hydrochloride salt (AA4-3) .
• a dry 500 mL round bottom flask under nitrogen atmosphere was charged with 60 ml of anhydrous CH 3 CN followed by N-methyl trimethylsilylamine ( AA4-0 , synthesized as described in Step 4-6, 1.9 mL, 13.3 mmol, 1.5 eq.).
• Step 4-3 Z-Dap(Boc-Me)-OH (AA4-4).
• AA4-3 To a flask containing the crude AA4-3 (8.9 mmol, 1.0 eq based on theoretical yield) was added 90 mL of anhydrous DCM. The mixture was cooled to 0°C and diisopropylethylamine (DIPEA, 7.8 mL, 44.5 mmol, 5.0 eq) added dropwise, which helps to solubilize the crude substrate.
• DIPEA diisopropylethylamine
• Di-tert-butyl dicarbonate 2.1 g, 9.8 mmol, 1.1 eq
• Step 4-4 H-Dap(Boc-Me)-OH (AA4-5).
• a solution of AA4-4 (2.5 g, 7.1 mmol, 1.0 eq.) in 75 mL of MeOH was added to 10% palladium on activated carbon (250 mg, 10% w/w) under a nitrogen atmosphere. Hydrogen was then bubbled into the mixture until completion of the reaction. [TLC BuOH:AcOH:H 2 O (4:1:1), UV, ninhydrin].
• the mixture was then purged with nitrogen, filtered on a Celite ® (World Minerals Inc., Santa Barbara, CA) pad and rinsed well with MeOH (3 x 25 mL). The filtrate was evaporated under reduced pressure and dried under vacuum to give 1.5 g (93%) of AA5-5 as a pale yellow solid.
• Step 4-5 Ddz-Dap(Boc-Me)-OH (Ddz-AA4).
• AA4-5 1.9 g, 8.7 mmol, 1.0 eq
• MeOH MeOH
• Triton B 4.3 mL, 9.5 mmol, 1.1 eq
• the mixture was stirred 30 min at room temperature, then concentrated under reduced pressure.
• the residue was azeotroped with toluene (2x) to remove excess H 2 O and MeOH.
• the resulting slurry was dissolved in 20 mL of anhydrous DMF and Ddz-OPh (3.1 g, 9.5 mmol, 1.1 eq) added in one portion.
• the mixture was stirred 36-48 h at 50°C under nitrogen. DMF was then removed under high vacuum and the residue diluted in H 2 O (50 mL). The resulting aqueous phase (checked to ensure pH was 9 and adjusted if necessary) was washed with Et 2 O until TLC indicates no phenol in the aqueous phase.
• the basic aqueous phase was cooled to 0°C, Et 2 O (50 mL) added, and the aqueous phase acidified with citrate buffer (1.0 M, pH 3.5). The separated aqueous phase was extracted with Et 2 O (2 x 50 mL).
• Step 4-6 Synthesis of N-methyl trimethylsilylamine (AA4-0).
• Methylamine (25.0 mL, 0.56 mol, 2.5 eq) was condensed at -20°C, then added to 250 mL of anhydrous Et 2 O at - 20°C under a nitrogen atmosphere.
• Freshly distilled (from LAH) chlorotrimethylsilane (28.1 mL, 0.22 mol, 1.0 eq) was added dropwise to the solution at -20°C. A white precipitate was formed.
• the reaction was warmed to room temperature and stirred for 4 h. The mixture was then cooled to 0°C and filtered cold.
• Step 5-1 3-(3-Fluorophenyl)-2-hydroxy-propionic acid benzyl ester (F1-2).
• Sodium nitrite (3.77 g, 54.6 mmol) was added in portions to a solution of L-3-fluorophenylalanine (F1-1, 5.0 g, 27.3 mmol) in acetic acid (82 mL) which is maintained at room temperature by immersion in an ice-water bath bearing a thermocouple. The solution is stirred at room temperature for 1 hr, then concentrated under reduced pressure. Water was then added to the residue and the product extracted with Et 2 O, dried over MgSO 4 , filtrated and concentrated under reduced pressure to yield 2.57g (42%) of the corresponding acid.
• Step 5-2 ⁇ 3-[2-(2-Amino-3-methyl-butoxy)-phenyl]-propyl ⁇ -carbamic acid tert-butyl ester (F1-5).
• Diisopropyl azodicarboxylate (DIAD, 3.13 mL, 15.9 mmol) is added dropwise to a well-stirred solution of triphenylphosphine (4.17 g, 15.9 mmol) in THF (150 mL) at 0°C under nitrogen. The mixture is stirred at 0°C for 30 min, then warmed to room temperature.
• the PPh 3 -DIAD adduct should precipitate as a white solid.
• Step 5-3 2- ⁇ 1-[2-(3- tert -Butoxycarbonylaminopropyl)-phenoxymethyl]-2-methyl-propyl-amino ⁇ -3-(3-fluorophenyl)-propionic acid benzyl ester F1.
• Freshly distilled (CF 3 SO 2 ) 2 O (29.5 ⁇ L, 175 ⁇ mol) was added to a solution of the alcohol F1-2 (45 mg, 164 ⁇ mol) in CH 2 Cl 2 at 0°C over 5 min, followed by 2,6-lutidine (22 ⁇ L, 189 ⁇ mol).
• the related fragment F2 was made following the same synthetic route as described for fragment F1 as illustrated in Figure 4 .
• Step 7-2 Ddz-amino-alkyne [T38-4(S)].
• T38-2(S) (23.9 g, 86.0 mmol, 1.0 eq)
• Distilled (after being stirred O/N with CaH 2 ) triethylamine (45 mL) was then added to the solution and the mixture purged by bubbling with argon for 5 min.
• Step 7-3 Ddz-Amino-alcohol [Ddz-T38(S)].
• T38-4(S) 35.0 g, 81.9 mmol, 1.0 eq
• EtOH 400 mL
• platinum oxide 1.86 g, 8.2 mmol, 0.1 eq
• Hydrogen was bubbled into the mixture O/N while stirring. Reaction was monitored by 1 H NMR until completion. When the reaction was complete, nitrogen was bubbled for 10 min to remove the excess of hydrogen.
• T38(S) is used to make compounds of formula I with an (R)-stereocenter.
• T38(R) is used to synthesize compounds of formula I with an (S)-stereocenter as described further in Examples 10 and 11.
• protected derivatives of tethers T92 and T93 can be obtained using analogous methods to that of Example 7 starting from 3-fluoro-2-iodophenol and 6-fluoro-2-iodophenol, respectively, or the corresponding 2-bromophenols.
• Step 8-2 3-(2-Methoxy-phenyl)-2-methyl-propionic acid ethyl ester (T81-3).
• T81-2 7.3 g, 23 mmol
• ethanol 100 ml
• Pd/C 730 mg
• Hydrogen was bubbled through the solution, then the solution stirred under hydrogen atmosphere for O/N.
• the suspension was filtered through a pad of silica gel and washed with ethyl acetate.
• the combined filtrate and washes were concentrated under reduced pressure to give T81-3 which was suitable for use in the next step without any further purification (yield: 86-90%).
• Step 8-3 3-(2-Methoxy-phenyl)-2-methyl-propan-1-ol (T81-4).
• An addition of water (18.8 mL) was effected very slowly (under a nitrogen flow) followed by a solution of 15% sodium hydroxide (18.8 mL), then water (56 mL).
• the residual salt was filtered and the filtrate concentrated under reduced pressure to give alcohol T81-4 (99%) which was used without any further purification in the next step.
• Step 8-4 Acetic acid 3-(2-methoxy-phenyl)-2-methyl-propyl ester [T81-5(S)] and 3-(2-Methoxy-phenyl)-2-methyl-propan-1-ol [T81-5(R)] .
• Enzyme Amano PS (1.7 g) and racemic alcohol T81-4 (30.7, 0.67 mol) were mixed with TBME (292 mL) in a reaction flask containing a magnetic stirring bar with no cap.
• TBME was placed in a dessicator and used to restore the volume of reaction after 24 h (due to loss by evaporation).
• the mixture was stirred for 24 h in a dessicator in the presence of saturated MgCl 2 (5-10 mL of a saturated solution of MgCl 2 in water was prepared and stored in the dessicator without any cap). After 24 h, the pre-equilibrated solvent was added to the reaction flask to compensate for loss by evaporation. Vinyl acetate (56 mL, 3.7 equiv) was added to the reaction flask, which was immediately sealed with a septum. The mixture was stirred at room temperature until a conversion of 42% was obtained as determined by LC-MS (aliquot starting after 2 h up to 5.5 h at which time the reaction was typically complete). The reaction was filtered through a medium fritted glass-sintered funnel.
• the alcohol T81-5(R) was retreated with the Amano PS with the same procedure used for the racemic alcohol except that the reaction was permitted to proceed until a conversion of 20% as determined by LC-MS (aliquots starting at 2 h, usually reached this level after 8.5 h). Then the mixture was treated as described for T81-5(S) to give the alcohol T81-6(R) (14.1 g) and the acetate T81-5(S). ( J. Chem. Soc., Perkin Trans. 1 2000, 367 .) This alcohol can be substituted for the T81-6(S) isomer in Step 8-6 below and transformed into T81 containing an 8R-stereocenter.
• Step 8-5 3-(2-Methoxy-phenyl)-2-methyl-propan-1-ol [T81-6(S)].
• Methanol (135 mL, 4x the volume of BBr 3 ) was added slowly at 0°C, followed by water.
• Step 8-6 3-[2-(2-Hydroxy-propoxy)-phenyl]-2-methyl-propan-1-ol [T81-7(1R,8S)].
• DMF 16 mL
• potassium carbonate 1.6 g, 8.42 mmol, 1 eq
• Step 8-7 Toluene-4-sulfonic acid 3-[2-(2-hydroxy-propoxy)-phenyl]-2-methyl-propyl ester [T81-8(1R,8S)].
• Step 8-8 1-[2-(3-Azido-2-methyl-propyl)-phenoxy]-propan-2-ol [T81-9(1R,8S)].
• T81-8(1R,8S) 2.2 g, 5.85 mmol, 1.0 eq
• NaN 3 1.85 g, 0.03 mol, 5 eq
• Step 8-9 1-[2-(3-Amino-2-methyl-propylpphenoxy]-propan-2-ol [T81-10 (1R,8S)].
• T81-9((1R,8S) (1.20 g, 4.8 mmol, 1.0 eq) in ethyl acetate (25 mL) was carefully added 10% Pd/C (0.12 g). Hydrogen was bubbled continuously through the solution and the resulting mixture stirred at room temperature O/N.
• Step 8-10 13-[2-(2-Hydroxy-propoxy)-phenyl]-2-methyl-propyl ⁇ -carbamic acid tert-butyl ester [Boc-T81 (1R,8S)].
• T81-10(1R,8S) (1.12 g, 5.01 mmol, 1.0 eq) in THF:H 2 O (1:1, 20 mL) was added K 2 CO 3 (1.43 g, 10.0 mmol, 2.0 eq) and Boc 2 O (3.30 g, 15.0 mmol, 3 eq).
• the mixture was stirred at room temperature O/N.
• the reaction was monitored by TLC [(ethyl acetate/hexanes, 4/6).
• T81(1R;8S) and T81(1R,8R) are used to make compounds of formula I with a (1S)-stereocenter.
• T81(1S,8S) and T81(1S,8R) are used to synthesize compounds of formula I with a (1R)-stereocenter.
• Step 9-1 In a round bottom flask equipped with a Dean-Stark apparatus, was suspended amino acid G-1 (0.2 mol, 1.0 eq) in toluene (1 L per 0.2 mol, solvent/wash amounts in the procedure are scaled for smaller or larger scales).
• p-Toluenesulfonic acid (pTSA, 1.2 eq) and benzyl alcohol (5.0 eq) were added and the mixture was stirred at reflux 16-18 h. The mixture was cooled down to room temperature and a white precipitate was formed. The precipitate was diluted with MTBE (500 mL), filtered and triturated with MTBE (3 x 500 mL). The solid was dried under high vacuum for 16-18 h to give the intermediate tosylate salt.
• the tosylate salt was taken up in an aqueous solution of 1 M Na 2 CO 3 (200 mL).
• the basic aqueous phase was extracted with EtOAc (4 x 150 mL) and the combined organic phases were washed with brine (1 x 100 mL), dried over MgSO 4 , filtered, concentrated under reduced pressure and dried in vacuo to give the free amino-ester G2.
• esters to the benzyl ester can also be employed for the procedure with modification of the deprotection protocol.
• Step 9-2 To a solution of freshly free based G-2 (1.0 eq.) in DCM (90-100 mL) was added triethylamine (TEA, 1.1 eq), pyridine (3.4 eq) and Bts-Cl (2.0 eq). The reaction was stirred O/N at room temperature. The reaction mixture was diluted with DCM (100 mL), washed sequentially with aqueous citrate buffer (2x), brine (1x), dried over MgSO 4 , filtered and concentrated under reduced pressure. The resulting residue was purified by flash chromatography to give Bts protected amino-ester G-3.
• TEA triethylamine
• pyridine 3.4 eq
• Bts-Cl 2.0 eq
• Step 9-3 Protected tether G-4 (1.5 eq.), triphenylphosphine (8.5g, 32.4 mmol, 1.5 eq) and G-3 (1.0 eq) were dissolved in anhydrous THF (100 mL). The solution was cooled to 0°C in an ice-water bath and diisopropylazodicarboxylate (DIAD, 1.45 eq) was added dropwise. The preformed DIAD:PPh 3 adduct (Example 38) could be used for this step as well. After addition, the mixture was stirred 1 h at 0°C, then warmed up slowly to room temperature and stirred for 16-18 h under nitrogen. THF was removed under reduced pressure and the crude residue was purified by flash chromatography to give alkylated amino acid ester G-5.
• DIAD diisopropylazodicarboxylate
• Step 9-4 To a solution of G-5 (1.0 eq) in DMF (75 mL), was added solid sodium carbonate (5.0 eq), followed by 2-mercaptoethanol (5.0 eq). The mixture was stirred for 16-18 h at room temperature. Reaction was monitored by HPLC (Grad_A4). Water (150 mL) was added and the aqueous phase extracted with EtOAc (3 x 75 mL). The combined organic phases were washed with brine (1 x 100mL), dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude residue was purified by flash chromatography to give debetsylated amino acid ester G-6. It is important to make sure no benzothiazole:mercaptoethanol adduct is left as determined by LC-MS after the reaction as this side product has been shown to inhibit the subsequent hydrogenolysis reaction.
• Step 9-5 This step is described for approximately 15 mmol scale.
• a round bottom flask under nitrogen atmosphere was charged with 10% Pd/C (50% wet, 10% by weight) and 95% EtOH (70 mL), followed by a solution of G-6 (15 mmol, 1.0 eq) in EtOH 95% (70 mL).
• the mixture was stirred under hydrogen bubbling using a gas dispersion tube for 4 hours. A precipitate formed in the mixture after 2 h. EtOAc or THF (100 mL) was added to obtain a stirring suspension.
• Step 9-6 For a salt of the amino acid benzyl ester ( G-8, 1 eq, described for 15 mmol scale), the solid was taken up in an aqueous solution of 1 M Na 2 CO 3 (50 mL). The basic aqueous phase was extracted with EtOAc (4 x 50 mL) and the combined organic phases were washed with brine (1 x 600 mL), dried over MgSO 4 , filtered, concentrated under reduced pressure and dried under high vacuum 4-6 h to give the free amino-ester G-9. Step 9-7.
• the organic phase was washed sequentially with an aqueous solution of citrate buffer (1 M, pH 3.5) (2 x 50 mL), H 2 O (1 x 50 mL), an aqueous solution of saturated NaHCO 3 (2 x 50 mL) and brine (1 x 50 mL).
• the organic phase was dried over MgSO 4 , filtered, concentrated under reduced pressure and dried under high vacuum 16-18 h to give crude dipeptide.
• Step 9-8 To a solution of acid fragment G-7 (1.0 eq) and dipeptide hydrochloride salt G-11 (1.05 eq) in anhydrous THF/DCM (1:1) (10 mL, described for 1.50 mmol) at 0°C was added DIPEA (5.0 eq), followed by HATU (1.05 eq). The mixture was stirred 1 h at 0°C, allowed to warm to room temperature and stirred 16-18 h under nitrogen. Solvent was removed under reduced pressure and the residue dissolved in EtOAc (50 mL).
• the organic phase was washed sequentially with an aqueous solution of citrate buffer (1 M, pH 3.5) (2 x 25 mL), an aqueous solution of saturated NaHCO 3 (2 x 25 mL) and brine (1 x 25 mL).
• the organic phase was dried over MgSO 4 , filtered and concentrated under reduced pressure.
• the crude residue was purified by flash chromatography to give the desired protected alkylated tripeptide G12.
• Step 9-9 A round bottom flask under a nitrogen atmosphere was charged with 10% Pd/C (50% wet, 10% weight) and 95% EtOH 95% (5 mL, described for 1.30 mmol), followed by a solution of G-12 (1.0 eq) in 95% EtOH (8 mL). The mixture was stirred under hydrogen bubbling for 4 h. The reaction mixture turned very thick after 2-3 h. THF was added to dissolve, at least partially, the suspension. The mixture was purged with nitrogen for 5 min to remove the excess of hydrogen, filtered on a Celite ® (World Minerals Inc., Santa Barbara, CA) pad and washed with THF until there was no further material eluting. The solvent was evaporated under reduced pressure and the resulting solid dried under high vacuum to give acid G-13.
• Step 9-10 Boc protected G-13 (1.0 eq, described for 1.30 mmol) was dissolved in TFA/TES/DCM (33/3/64) (9.8 eq, approximately 3 mL). The mixture was stirred 30 min at room temperature and evaporated under reduced pressure. The residue was co-evaporated twice with THF, then dried under high vacuum for 16-18 h to give macrocyclic precursor G-14.
• Step 9-11 To a solution of G-14 (1.0 eq, described for 1.30 mmol) in anhydrous THF (sufficient to form a 25 mM solution, although solutions of 10 mM concentration are also acceptable) were added DIPEA (5.0 eq) and DEPBT (1.1 eq). The mixture was stirred at room temperature 16-18 h. THF was evaporated under reduced pressure and the residue was taken up in 1 M Na 2 CO 3 (aq):EtOAc (1:1) (50 mL). The separated basic aqueous phase was washed with brine (2 x 25 mL), dried over MgSO 4 , filtered and evaporated under reduced pressure. The crude residue was purified by flash chromatography or reverse phase HPLC to give the macrocycle G-15.
• Table 13 below lists the yields of this final macrocyclization step for other representative compounds of the invention that have been synthesized according to this standard procedure.
• Table 13 Representative Yields for Step 9-11 Compound Macrocyclization Yield a Concentration 513 73% 25 mM 554 75% 25 mM 555 78% 25 mM 557 76% 25 mM 558 72% 25 mM 559 83% 10 mM 560 73% 10 mM 561 55% 10 mM 562 87% 10 mM a Yield determined after silica gel purification, based on linear precursor prior Boc deprotection.
• Step 10-1 Chlorination of H-(D)Tyr-OMe.
• Step 10-2 Double Bts protection. To a solution of 10-2, HCl salt in DCM (50 mL) was added pyridine (5.8 mL, 68 mmol, 6.8 eq) and Et 3 N (3.0 mL, 22 mmol, 2.2 eq), followed by solid Bts-Cl (9.3 g, 40 mmol, 4 eq). The mixture was then stirred for 16 h at rt. It was diluted with DCM (100 mL), washed sequentially with citrate buffer (2 x 50 mL), saturated aqueous sodium bicarbonate (2 x 50 mL), brine once then dried on MgSO 4 and concentrated.
• Step 10-3 Fukuyama-Mitsunobu.
• Boc-T38(R) 2.2 g, 7.2 mmol, 1.4 eq
• PPh 3 1.9 g, 7.2 mmol, 1.4 eq
• DIAD 1.5 mL, 7.2 mmol, 1.4 eq
• Step 10-4 Debetsylation.
• sodium carbonate 3.3 g, 31 mmol, 5 eq
• 2-mercaptoethanol 2.2 mL, 31 mmol, 5 eq
• the mixture was then stirred for 16 h under N 2 , then DMF was evaporated and the mixture was diluted with water (200 mL) and pH was adjusted to 8.
• the aqueous layer was extracted with EtOAc (3 x 100 mL). The combined organic layers were washed with brine, then dried with MgSO 4 and concentrated under reduced pressure.
• Step 10-5 Ester hydrolysis.
• Step 10-6 Fragment coupling.
• 10-6 (1.94 g, 3.8 mmol, 1.0 eq) and 10-7 (synthesized as described in Step 10-9, 1.24 g, 4.6 mmol, 1.2 eq) in DMF (20 mL) were added HATU (1.75 g, 4.6 mmol, 1.2 eq) and DIPEA (4.0 ml, 23 mmol, 5 eq.).
• the mixture was stirred for 16 h at rt under N 2 , then the solvent evaporated under reduced pressure.
• the residue was taken up in water (20 mL), the pH adjusted to 8-9 and the product extracted with EtOAc (2 x 20 mL) and DCM (2 x 20 mL).
• Step 10-7 Deprotections. To 10-8 (1.5 g, 2.0 mmol) was added a mixture of TFA (70 mL), DCM (30 mL) and Et 3 SiH (3 mL). The mixture was stirred at rt for 2 h, then concentrated under reduced pressure. It was evaporated twice with DCM, then dried in vacuo. The crude residue, 10-9, was azeotroped with THF (2x) prior to macrocyclization in Step X-8.
• Step 10-8 Macrocyclization.
• DEPBT 1.2 g, 4.0 mmol, 2 eq
• DIPEA 4.2 mL, 24 mmol, 6 eq
• THF was removed and the product purified by flash chromatography (100% EtOAc) to give compound 552 as a white foam (0.80 g, 68.3% for two steps).
• Similar yields (70.9%) and purity (89.3/91.1/100, UV, CLND, ELSD) were obtained by modifying the above procedure to replace the flash chromatography with passage through a silica gel pad.
• LC-MS UV, CLND, ELSD
• 92.9/100/98.6; t R 5.84 min; (M+H) + 586.
• Compound 552 was dissolved in acetonitrile (60 mg.mL) and 1.5 eq. 0.5 M HCl (aq) added to immediately form a solid precipitate. The solid was collected by filtration and washed with acetonitrile. The resulting HCl salt of compound 552 can be recrystallized one or more times from 95% EtOH using heptane to induce solid formation.
• Step 11-1 In a round bottom flask equipped with a Dean-Stark apparatus, was suspended H-(D)Phe(3F)-OH ( 11-1 , 35.6 g, 194 mmol, 1.0 eq) in toluene (1 L). para Toluenesulfonic acid (44.3 g, 233 mmol, 1.2 eq), benzyl alcohol (101 mL, 970 mmol, 5.0 eq) were added and the mixture was stirred at reflux 16-18 hours. The mixture was cooled down to room temperature and a white precipitate formed. The precipitate was diluted with MTBE (500 mL), filtered and triturated with MTBE (3 x 500 mL). The solid was dried under high vacuum for 16-18 h to give the tosylate salt as a white solid (85.1 g, 98.6%).
• the tosylate salt (85.1 g) was taken up in an aqueous solution of 1 M Na 2 CO 3 (210 mL). The basic aqueous phase was extracted with EtOAc (4 x 150 mL), then the combined organic phases were washed with brine (1 x 100 mL), dried over MgSO 4 , filtered, concentrated under reduced pressure and dried under high vacuum to give the free amino-ester 11-2 as a clear oil (49.5 g, 95%).
• Step 11-2 To a solution of freshly free based 11-2 (49.5 g, 181 mmol, 1.0 eq) in DCM (90 mL) was added triethylamine (27.8 mL, 616 mmol, 1.1 eq), pyridine (49.8 mL, 616 mmol, 3.4 eq) and Bts-Cl (84.7 g, 363 mmol, 2.0 eq). The reaction was stirred O/N at room temperature during which time the solution turned orange. The reaction mixture was diluted with DCM (100mL), washed sequentially with citrate buffer (2x) and brine (1x), dried over MgSO 4 , filtered and concentrated under reduced pressure.
• Step 11-3 Boc-T38(R) tether ( 11-4, 10.0 g, 32.4 mmol, 1.5 eq), triphenylphosphine (8.5 g, 32.4 mmol, 1.5 eq) and Bts-(D)Phe(3F)-OBn ( 11-3, 10.2 g, 21.6 mmol, 1.0 eq) were dissolved in anhydrous THF (108 mL). The solution was cooled to 0°C in an ice-water bath and diisopropylazodicarboxylate (DIAD, 6.2 mL, 31.3 mmol, 1.45 eq) was added dropwise.
• DIAD diisopropylazodicarboxylate
• Step 11-4 To a solution of alkylated amino acid 11-5 (14.0 g, 18.4 mmol, 1.0 eq) in DMF (74 mL), was added sodium carbonate (9.8 g, 92.0 mmol, 5.0 eq), followed by 2-mercaptoethanol (6.5 mL, 92.0 mmol, 5.0 eq). The mixture was stirred for 16-18 h at room temperature. Reaction was monitored by HPLC [(Grad_A4): 9.69 min (product), 15.37 min (starting material)]. Water (150 mL) was added and the aqueous phase extracted with EtOAc (3 x 75mL).
• Step 11-5 A round bottom flask under nitrogen atmosphere was charged with 10% Pd/C (50% wet, 785 mg, 10% by weight) and 95% EtOH (70 mL), followed by a solution of amino acid ester 11-6 (7.85 g, 13.9 mmol, 1.0 eq) in 95% EtOH (70 mL). The mixture was stirred under hydrogen bubbling using a gas dispersion tube for 4 h. A precipitate formed after 2 h. EtOAc (100 mL) was added to obtain a stirring suspension.
• Step 11-6 Commercially available H-Leu-OBn tosylate salt (11-8, 6.1 g) was taken up in an aqueous solution of 1 M Na 2 CO 3 (50 mL). The basic aqueous phase was extracted with EtOAc (4 x 50 mL) and the combined organic phases were washed with brine (1 x 580 mL), dried over MgSO 4 , filtered, concentrated under reduced pressure and dried under vacuum 4-6 h to give the free amino-ester 11-9 as a pale yellow oil (3.44 g, 100%).
• Step 11-7 Freshly free based H-Leu-OBn ( 11-9, 3.44 g, 15.5 mmol, 1.0 eq) and Boc-(D)Val-OH (11-10, 3.54 g, 16.3 mmol, 1.05 eq) were dissolved in anhydrous THF:CH 2 Cl 2 (1:1, 81 mL). 6-Cl-HOBt (2.63 g, 15.5 mmol, 1.0 eq) and DIPEA (14.2 mL, 81.5 mmol, 5.0 eq) were added. The mixture was cooled to 0°C in an ice-water bath and EDC (3.28 g, 17.1 mmol, 1.1 eq) was added.
• Step 11-8 To a solution of 11-7 (750 mg, 1.58 mmol, 1.0 eq) and 11-11 (591 g, 1.66 mmol, 1.05 eq) in anhydrous THF:DCM (1:1, 10.5 mL) at 0°C was added DIPEA (1.4 mL, 7.9 mmol, 5.0 eq), followed by HATU (631 mg, 1.66 mmol, 1.05 eq). The mixture was stirred 1 h at 0°C, allowed to warm to room temperature and stirred 16-18 h under nitrogen. Solvent was removed under reduced pressure and the residue was dissolved in EtOAc (50 mL).
• Step 11-10 Boc protected alkylated tripeptide 11-13 (911 mg, 1.33 mmol, 1.0 eq) was dissolve in a TFA/TES/DCM (33/3/64, 3.0 mL, 12.9 mmol, 9.8 eq). The mixture was stirred 30 min at room temperature and evaporated under reduced pressure. The oily residue was co-evaporated twice with THF, then dried under high vacuum for 16-18 h to give macrocyclic precursor 11-14 as a pale yellow gummy solid (1.27 g, > 100%).
• TLC: [EtOAc:MeOH (9:1)]: R f baseline (UV, ninhydrin).
• Step 11-11 To a solution of macrocyclic precursor 11-14 [1.27 g, 1.33 mmol (based on quantity obtained after hydrogenolysis), 1.0 eq) in anhydrous THF (53.6 mL, for a 25 mM solution) were added DIPEA (1.17 mL, 6.79 mmol, 5.0 eq) and DEPBT (440 mg, 1.47 mmol, 1.1 eq). The mixture was stirred at room temperature 16-18 h. THF was evaporated under reduced pressure and the residue was taken up in 1 M Na 2 CO 3 :EtOAc (1:1, 50 mL).
• Step 15-1 (Panda, G.; Rao, N. V. Synlett 2004 , 714.) To a stirred suspension of Boc-L-Ser-OH ( 15-1 , 5.0 g, 24.5 mmol, 1.0 eq) in 160 mL of anhydrous DMF at room temperature was added N,N-diisopropylethylamine (DIPEA, 4.25 mL, 24.5 mmol, 1.0 eq).
• DIPEA N,N-diisopropylethylamine
• Step 15-2 To a mixture of the alcohol 15-2 (5.0 g, 20 mmol, 1 eq) and triethylamine (5.60 mL, 40.3 mmol, 2 eq) in 100 mL of anhydrous dichloromethane under N 2 , was added freshly distilled methanesulfonyl chloride (2.35 mL, 30 mmol, 1.5 eq) dropwise over 5 min. The reaction was allowed to warm to room temperature and stirred for 3 h. The mixture was washed sequentially with cold water, 1 M citrate buffer, saturated aqueous sodium bicarbonate, and brine.
• Step 15-3 To a solution of 15-3 (5.0 g, 15.3 mmol, 1 eq) in 60 ml of anhydrous DMF was added sodium azide (3.0 g, 46 mmol, 3 eq) under an argon atmosphere and the mixture stirred at 50°C for 12 h. ( CAUTION! Such quantities of NaN 3 should be handled carefully . ). The reaction mixture was cooled to 0°C and water (100 mL) added very slowly. The reaction mixture was extracted with ethyl acetate (3 x 100 mL).
• Such amino macrocycles can also be synthesized by assembly with appropriately protected Dap derivatives using Example 9 or the solid phase procedure illustrated in Figure 7 .
• the reaction was cooled to -78°C and an excess of propylene gas was bubbled (over 10 eq) into the solution and the flask sealed. The mixture was stirred at room temperature O/N. The reaction was cooled to 0°C, propylene was released slowly, and Cu was filtered through Celite ® (World Minerals Inc., Santa Barbara, CA) and washed with acetonitrile. All solvents were removed under reduced pressure. The resulting residue was purified by purified by flash chromatography (ethyl acetate:methanol, 95:05) to give 526 (76% yield).
• Step 25-1 To a solution of 17-2 (1.0 g, 1.73 mmol, 1 eq) in DCM (10 mL) at room temperature was added Bts-Cl (0.41 g, 1.73 mmol, 1 eq), and triethylamine (0.175g, 1.73 mmol, 1 eq). The mixture was stirred at room temperature O/N. The reaction mixture was washed with 0.1 N HCl, dried over magnesium sulfate and concentrated to dryness under reduced pressure. The resulting residue was purified by flash chromatography (EtOAc:hexanes, 1:1) to give 25-1 (95% yield).
• Step 25-2 To a solution of 25-1 (50 mg, 64.5 ⁇ mol, 1 eq) in THF (10 mL) at room temperature was added methanol (26 ⁇ L, 0.645 mmol, 10 eq), the pre-formed triphenylphosphine-DIAD adduct (synthesized as described in Example 38, 32 mg, 0.071 mmol, 1.1 eq). The mixture was stirred at room temperature O/N. The reaction mixture was concentrated to dryness under reduced pressure. The residue was purified by flash chromatography (ethyl acetate:hexanes, 1:1) to give 25-2 (98% yield).
• Step 25-3 25-2 (50 mg) was dissolved in ethanol:THF (1:1,2 mL) at room temperature, treated with thiophenol resin (1 g) and agitated on an orbital shaker for 2 h. The resin was filtered off and washed several times with ethanol:THF. The filtrate and washings were removed under reduced pressure and the crude residue subjected to HPLC purification to give 25-3 (50% yield).
• Compound 546 was synthesized from 17-20 using the standard procedure of Example 25 in ⁇ 44% overall yield for the three step sequence.
• Compound 548 was synthesized from 17-13 using the standard procedure of Example 25 in 40% overall yield for the three step sequence.
• Monomethylamino-containing macrocycles are also synthesized using building block Boc-AA4 in solid phase methods as illustrated in Figure 7 or in solution phase procedures as described in Example 9.
• Compound 550 was synthesized from X-13 using the standard procedure of Example 26 in 80% yield.
• Step 27-1 To a solution of 27-1 (synthesized from the corresponding macrocycle containing a free secondary amine and a protected primary amine by treatment with Bts-Cl similarly to as described in Step 11-2 followed by deprotection of the Boc under standard conditions, 3.3 g, 3.63 mmol, 1 eq) in TFA (50 mL, 0.653 mol, 180 eq) at room temperature was added polystyrene (PS) sulfonamide resin (Argonaut Technologies, now part of Biotage AB, Uppsala, Sweden, 1.1 mmol/g,, 16.8 g, 18.2 mmol, 5 eq). The mixture was stirred at 70°C for 2 h. The resin was filtered off and washed with TFA and DCM several times. The filtrate and washings were removed under reduced pressure. The resulting residue was purified by flash chromatography (100% ethyl acetate) to give 27-2 (70% yield).
• PS polysty
• Step 27-2 To a solution of 27-2 (1.2 g, 1.55 mmol, 1 eq) in acetonitrile/TMOF (6 mL/2 mL) at room temperature was added formaldehyde (0.426 ml, 15.5 mmol, 10 eq), NaBH 3 CN (97 mg, 15.5 mmol, 10 eq) and 1 drop of acetic acid. The reaction was stirred at room temperature for 2 h. The solution was cooled to 0°C, then 1 mL of a solution of 25% NaOH slowly added and the reaction stirred for 5 min.
• Step 27-3 To a solution of 27-3 (1.33 g, 1.66 mmol, 1 eq) in DMF (10 mL) at room temperature was added mercaptopropanoic acid (0.9 g, 8.30 mmol, 5 eq), and K 2 CO 3 (1.15 g, 8.30 mmol, 5 eq). The mixture was stirred at room temperature for 12 h. The reaction mixture was diluted with ethyl acetate and water, then extracted with ethyl acetate. The combined organic layers were washed with brine, dried over magnesium sulfate and concentrated to dryness under reduced pressure. The resulting residue was purified by flash chromatography (ethyl acetate:methanol, 9:1) to give 27-4 (80% yield).
• Step 28-1 To a solution of 538 (0.200 g, 0.352 mmol, 1 eq) in DCM (2 mL) at room temperature were added trifluoroacetic anhydride (TFAA, 122 ⁇ L, 0.882 mmol, 2.5 eq) and triethylamine (TEA, 250 ⁇ L, 1.76 mmol, 5 eq). The reaction was stirred at 0°C for 1 h. The mixture was then diluted with 10 mL of dichloromethane and washed with 0.1 N HCl and brine, dried over magnesium sulfate and concentrated to dryness under reduced pressure. The residue was purified by flash chromatography (ethyl acetate:hexanes, 1:1) to give 28-1 (35% yield)
• Step 28-2 To a solution of 28-1 (66 mg, 0.0994 mmol, 1 eq) in ethyl acetate (5 mL) at room temperature was added Pd/C (15 mg, 20% by weight), and 1 drop of acetic acid. The mixture was stirred at room temperature under a hydrogen atmosphere using a balloon O/N. The Pd/C was filtered through Celite ® (World Minerals Inc., Santa Barbara, CA) and washed with ethyl acetate. The filtrate and washings were removed under reduced pressure to provide 28-2.
• Pd/C Celite ® (World Minerals Inc., Santa Barbara, CA)
• Step 28-3 The crude 28-2 was dissolved in acetonitrile/TMOF (6 mL/2 mL) at room temperature and formaldehyde (22 ⁇ L, 0.0995 mmol, 3 eq), NaBH 3 CN (6.15 mg, 0.0298 mmol, 2 eq) and 1 drop of acetic acid were added. The mixture was stirred at room temperature for 2 h. The solution was cooled to 0°C and 1 mL of a solution of 25% NaOH was slowly added and the mixture stirred for 5 min. The aqueous phase was extracted with ethyl acetate (3x), washed with brine, dried over MgSO 4 , filtered and concentrated under reduced pressure.
• Nitrile 33-1 obtained from solid phase synthesis with appropriate amino acid building blocks according to the procedure outlined in Figure 7 , 218 mg crude
• Nitrile 33-1 was dissolved in a solution of 1.25 M HCl in EtOH (25 mL) at 0°C and gaseous HCl was bubbled into the solution for 30 min. The mixture was then stirred at room temperature for 2 h. The solution was evaporated under reduced pressure, then dried under high vacuum. The crude residue was dissolved in a solution of 2 M ammonia in ethanol (25 mL) and the mixture stirred at room temperature O/N. The solution was evaporated under reduced pressure and purified by reverse phase HPLC to give 33-3 (17.4 mg). Removal of the Bts group was achieved as described for Example 31.
• Step 34-1 3-(2-(R) Hydroxyethyl-1-oxy)-2-bromopyridine [T94-2(R)].
• 2-Bromo-3-pyridinol T94-1 2.0 g, 12 mmol, 1 eq
• DMF anhydrous, 50 mL
• Potassium carbonate 1.6 g, 12 mmol, 1 eq
• (R)-methyl carbonate (1 mL, 12 mmol, 1 eq.
• the reaction was then heated to 85°C using an oil-bath for 72 h.
• Step 34-2 3-(2-(R) Hydroxyethyl-1-oxy)-2-(1- tert -butoxycarbonylamino-prop-2-yn-3-yl)pyridine [T94-3(R)].
• T94-2(R) (1 g, 4.3 mmol, 1 eq) and Boc-propargylamine derivative (1.1 g, 6.46 mmol, 1.5 eq) were dissolved in Et 3 N (distilled over CaH 2 ):DMF (1:3, 15 mL) and the reaction mixture was degassed by bubbling argon through the solution.
• Step 34-3 3-(2-(R)Hydroxyethyl-1-oxy)-2-(1- tert -butoxycarbonylaminoprop-3-yl)pyridine [Boc-T94(R)].
• the acetylenic compound T94-3(R) (5.0 g, 16 mmol) was dissolved in EtOH (80 mL), then PtO 2 (4 mol%, 150 mg) was added and the mixture stirred under a hydrogen atmosphere (a balloon full of hydrogen gas was used) O/N.
• Step 35-1 A solution of 3-hydroxypyridine ( T95-1 , 14.0 g, 0.147 mmol) in THF/DMF (315 mL/315 mL) was cooled to 0°C, then potassium tert -butoxide (24.7 g, 221 mmol) added in small portions. The mixture was stirred for 5 min, then MOM-Cl (13.0 mL, 162 mmol) added dropwise over 10 min. A saturated aqueous solution of ammonium chloride was added and the THF removed under reduced pressure. The aqueous phase was then extracted with Et 2 O (3x). The combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude T95-2 (orange oil) was used as obtained in the next step.
• Step 35-2 A solution of T95-2 (6.0 g, 43 mmol) and TMEDA (7.8 mL, 52 mmol) in THF (175 mL) was cooled to -78°C and agitated with a mechanical stirrer. nBuLi (1.56 M in hexane, 31.4 mL, 49 mmol) was added dropwise. A sticky orange thick syrup was formed and dissolved over time. The solution was stirred for 1 h then I 2 (14.2 g, 56 mmol) was added and the solution stirred another 1 h at -78°C then warmed up to rt O/N. Water was added to the reaction and the THF removed under reduced pressure.
• Step 35-3 To a solution of T95-3 (6.5 g, 24.6 mmol) in DCM (49 mL) at rt was added TFA (49 mL). The mixture was stirred for 2 h at rt. The solvent was removed under reduced pressure and re-treated with the same reagents under the same conditions for an additional hour. The reaction was monitored by 1 H NMR spectroscopy on a worked-up reaction aliquot. A saturated aqueous solution of sodium bicarbonate was added and the aqueous phase extracted with EtOAc (3x). The combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure to give 5.1 g (94%) of T95-4 as a yellowish solid, which was used as such for the following step.
• Step 35-4 To a solution of 3-hydroxy-4-iodopyridine (5.09 g, 23.0 mmol) in DMF (95 mL) were added (R)-propylene carbonate ( T95-A , 2.35 g, 23.0 mmol)) and potassium carbonate (3.18 g, 23.0 mmol). The solution was stirred at 85°C for 72 h. Water was added and the aqueous phase extracted with EtOAc (3x). The combined organic phases were washed with brine (1x) and water (1x), dried over MgSO 4 , filtered and concentrated under reduced pressure. The resulting residue was purified by flash chromatography (100% EtOAc) to give 1.4 g (24%) of T95-4(R) .
• Step 35-5 A solution of iodide T95-4(R) (1.27 g, 4.56 mmol) and Boc-propargylamine (1.06 g, 6.83 mmol) in CH 3 CN/TEA (13.2 mL/5.4 mL) was degassed for 5 min with argon, then Pd(Cl) 2 (PPh 3 ) 2 (95 mg) and Cul (25 mg) were added. The solution was heated at 50°C O/N. The solvent was removed under reduced pressure and the resulting residue was purified by flash chromatography (100% EtOAc) to give 1.23 g (89%) of T95-5(R) .
• T95(R) is used to make compounds of formula I with an (S)-stereocenter as described further in Example 37.
• T95(S) is used to synthesize compounds of formula I with an (R)-stereocenter.
• Step 36-1 To a solution of 4-hydroxypyridine ( T96-1 , 7.0 g, 74 mmol) in CCl 4 (360 mL) at rt was added NBS (26.2 g, 0.147 mol). The solution was stirred for 24 h in the dark (covered with aluminum foil). The mixture was concentrated under reduced pressure and the resulting residue triturated with MeOH, then with acetone to give 18.9 g (100%) of T96-2 .
• Step 36-2 To a solution of T96-2 (8.0 g, 31.3 mmol) in THF (307 mL) at -90°C was added slowly a solution of n-BuLi (1.15 M in hexanes, 71 mL). The mixture was stirred for 40 min, then water (10 eq) was added and the mixture warmed to room temperature. The solvent was removed under reduced pressure and the resulting residue purified by flash chromatography (acetone:MeOH, 10:1) to give 3.22 g (60%) of T 96-3 .
• Step 36-3 T 96-3 (3.22 g, 18.4 mmol), PPh 3 (4.82 g, 18.4 mmol) and the mono-PMB ether of (R)-glycerol ( T96-A , synthesized from the corresponding diol using standard methods, 1.8 g, 9.2 mmol) were dissolved in THF (14 mL). The mixture was cooled to 0°C, then DIAD (3.5 mL, 17.9 mmol) added dropwise maintaining the temperature at 0°C. The solution was stirred at 0°C for 1 h, then at rt O/N. The mixture was concentrated under reduced pressure and the residue purified by flash chromatography (50/50 EtOAc/Hex) to yield T96-4(R) (42%).
• Step 36-4 To a solution of T96-4(R) (1.25 g, 3.56 mmol) in DIPEA (20 mL) was added Boc-propargylamine and the mixture degassed with argon for 10 min. Triphenylphosphine (121 mg), CuI (29 mg) and Pd(Cl) 2 PPH 3 (160 mg) were then added. The solution was stirred at 70°C O/N. The reaction mixture was concentrated under reduced pressure and the resulting residue purified by flash chromatography (gradient, 40% to 90% EtOAc/Hexane) to give 1.3 g (80%) of T96-5(R) .
• Step 36-5 Pd/C (10%, 50% wet, 628 mg) was added to a solution of T96-5(R) (900 mg) and AcOH (1.5 eq) in 95% EtOH (20 mL). The solution was saturated with H 2 at 1300 psi. The mixture was filtered through Celite ® (World Minerals Inc., Santa Barbara, CA) and washed several times with EtOAc, then with MeOH. The combined filtrate and washings were concentrated under reduced pressure and the crude Boc-T96(R) obtained was used in the synthesis of macrocyclic compounds of the invention without any further purification.
• Celite ® World Minerals Inc., Santa Barbara, CA
• T96(R) is used to make compounds of formula I with an (S)-stereocenter.
• T96(S) is used to synthesize compounds of formula I with an (R)-stereocenter.
• Step 37-1 Boc-T95(R) (901 mg, 2.90 mmol), triphenylphosphine (762 mg, 2.90 mmol) and Bts-(D)Phe(3F)-OBn (976 mg, 2.07 mmol) were dissolved in anhydrous THF (10 mL). The solution was cooled to 0°C in an ice-water bath and diisopropylazodicarboxylate (DIAD, 551 ⁇ L) was added dropwise. Once the addition was completed, the mixture was stirred for 1 h at 0°C, then warmed up slowly to room temperature and stirred for an additional 16-18 h under nitrogen.
• DIAD diisopropylazodicarboxylate
• Step 37-2 To a solution of alkylated amino acid 37-1 (778 mg, 1.02 mmol) in DMF (6 mL) was added sodium carbonate (519 mg, 5 eq), followed by 2-mercaptoethanol (346 ⁇ L, 5 eq). The mixture was stirred for 16-18 hours at room temperature. Water was added and the aqueous phase was extracted with EtOAc (3x). The combined organic phases were washed with brine (1x), dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude residue was purified by flash chromatography (gradient 6/3 EtOAc/Hexane to 100% EtOAc) to give 552 mg of 37-2 (96%).
• Step 37-3 Pd/C (10%, 50% wet, 628 mg) was carefully added to a solution of 37-2 (502 mg, 0.888 mmol) and AcOH (2 drops) in 95% EtOH (6 mL). The solution was saturated with hydrogen (H 2 gas bubbled into the reaction mixture for 2 h), then stirred O/N under H 2 atmosphere. The mixture was filtered through Celite ® (World Minerals Inc., Santa Barbara, CA) and washed several times with EtOAc, then with MeOH. The filtrate was concentrated under reduced pressure and the crude 37-3 (94%) thus obtained was used in the next step without any further purification.
• Celite ® World Minerals Inc., Santa Barbara, CA
• Step 37-4 To a solution of the amino acid zwitterion 37-3 (293 mg, 0.617 mmol) and H-D-Val-Nva-OBn hydrochloride salt (422 mg, 1.23 mmol) in anhydrous THF (3.0 mL) at 0°C was added DIPEA (1.1 mL, 6.17 mmol), followed by HATU (469 mg, 1.23 mmol). The mixture was stirred for 16-18 h under nitrogen. The solvent was removed under reduced pressure and the resulting residue dissolved in EtOAc. The organic phase was washed sequentially with an aqueous solution of citrate buffer (1 M, pH 3.5, 2x), a saturated aqueous of sodium bicarbonate (2x) and brine (2x). The organic phase was dried over MgSO 4 , filtered and concentrated under reduced pressure. The residue thus obtained was purified by flash chromatography (80/20 EtOAc /Hex) to give 290 mg of 37-4 (62%).
• Step 37-5 Pd/C (10%, 50% wet, 28 mg) was added to a solution of 37-4 (284 mg, 0.373 mmol) in 95% EtOH (4 mL). The solution was saturated with hydrogen (H 2 gas bubbled into the reaction mixture for 2 h), then stirred O/N under a H 2 atmosphere. The mixture was filtered through Celite ® (World Minerals Inc., Santa Barbara, CA) and washed several times with EtOAc, then with MeOH. The filtrate was concentrated under reduced pressure and the crude 37-5 obtained, 256 mg (100%), was used in the next step without any further purification.
• Celite ® World Minerals Inc., Santa Barbara, CA
• Step 37-6 Boc protected alkylated tripeptide 37-5 (256 mg) was dissolved in a TFA/TES/DCM (33/3/64, 17 mL) mixture. The reaction was stirred I h at room temperature, then concentrated under reduced pressure. The resulting oily residue was co-evaporated with DCM (3x), then THF (3x) and dried under reduced pressure to give macrocyclic precursor 37-6 .
• Diisopropyl azodicarboxylate (DIAD, 1 eq) is added dropwise to a well-stirred solution of triphenylphosphine (1 eq) in THF (0.4 M) at 0°C under nitrogen. After completion of the addition, the mixture is stirred at 0°C for an additional 30 min. The white solid obtained is collected by filtration (use medium sized fritted filters) and washed with cold anhydrous THF until the washes are colorless. Finally, the white precipitate is washed once with anhydrous Et 2 O. The adduct is then dried well in vacuo , and stored under nitrogen. (It is important to store this reagent under anhydrous conditions! The adduct can be used in place of the separately added reagents in Mitsunobu-type reactions.
• Formulations designed to enhance the solubility of drugs often result in both increasing oral bioavailability and clinical efficacy.
• the most popular approaches incorporate the active lipophilic component into inert lipid vehicles ( Christopher J. H. Porter & al. Lipid and lipid-based formulation; optimizing the oral delivery of lipophilic drugs. Nat. Rev. Drug. Disc. 2007, 6, 231-246 ; Aungst, B.J. Novel formulation strategies for improving oral bioavailability of drugs with poor membrane permeation or presystemic metabolism. J. Pharm. Sci. 1993, 81, 979-987 ), such as oils ( Burcham, D.L.; Maurin, M.B.; Hausner, E.A.; Huang, S.M.

Abstract

Description