EP2981358A1 - Systeme de pipetage a commande et reglage de volume ameliores - Google Patents
Systeme de pipetage a commande et reglage de volume amelioresInfo
- Publication number
- EP2981358A1 EP2981358A1 EP14715882.8A EP14715882A EP2981358A1 EP 2981358 A1 EP2981358 A1 EP 2981358A1 EP 14715882 A EP14715882 A EP 14715882A EP 2981358 A1 EP2981358 A1 EP 2981358A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- pipetting
- rotation
- axis
- connecting member
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006073 displacement reaction Methods 0.000 claims abstract description 21
- 238000005070 sampling Methods 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- 210000003811 finger Anatomy 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- 241000920340 Pion Species 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0227—Details of motor drive means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
Definitions
- the present invention relates to the field of air displacement pipetting. It relates more particularly to a device for controlling pipetting and adjusting a volume to be taken.
- Pipettes also known as sampling pipettes, laboratory pipettes or liquid transfer pipettes, are a preferred application. They are intended for the collection and dispensing of liquid in containers or the like.
- the manual pipettes are intended to be held in the hand by an operator during the operations of sampling and dispensing liquid, these operations being performed by setting in motion a control knob obtained by the application of an axial pressure on this same button.
- the axial pressure applied to the control knob is transmitted to a piston of the pipette, which undergoes axial displacement and causes a displacement of air leading to sampling and dispensing operations.
- the displacement of the piston is carried out under the action of a motor driven by a device. electronic.
- the subject of the invention is a device for controlling the pipetting and adjustment of a volume to be sampled, for an air displacement pipetting system, said device comprising:
- Rotating actuating means coupled to the control member so that a translation displacement of the latter causes a rotation of the actuating means according to a first axis of rotation;
- rotary means defining, for the connecting member, a spiral-shaped guiding path, said rotary means being coupled to said adjusting means so that actuation thereof causes rotation of the rotary means along a second axis; rotation parallel to the first axis and on which is centered the spiral guide path, this rotation simultaneously leading to a relative displacement of the connecting member along the spiral guide path, and to a sliding of the connection relative to said actuating means.
- the invention is remarkable in that it provides for the implementation of a guiding path of the spiral-shaped connecting member, adapted for adjusting the volume to be sampled while allowing a constant pipetting stroke to be maintained. , whatever the volume taken.
- the spiral shape of the path guiding the connecting member allows it to describe a substantially circular movement that is particularly reliable, and little subject to errors and risks of blockage. It also provides high pipetting accuracy to the system.
- said actuating means comprise a toothed sector cooperating with a rack made on the pipetting control member.
- Other coupling means may nevertheless be envisaged, such as friction means, without departing from the scope of the invention.
- said actuating means comprise a finger provided with a groove in which the connecting member is slidably mounted.
- the connecting member is a pion.
- said first and second axes of rotation are substantially coincidental. Alternatively, they could remain parallel, at a distance from each other, without departing from the scope of the invention.
- said volume control means are rotatable.
- the adjustment means comprise a worm cooperating with a helical cut-out provided on the rotary means.
- These adjustment means are distinct from the control member, even if designs which nest these two elements can be retained, for example by arranging them coaxially around each other.
- the sliding axis of the connecting member is parallel to a translation axis of the control member.
- the sliding of the connecting member relative to said actuating means is effected along an axis parallel to the axis of translation of the actuator member.
- control which is generally parallel to the axis of sliding of the piston of the pipetting system.
- the passage from the high position of the control member to its low position causes a rotation of the actuating means at an angle substantially equal to 180 °.
- a lower angle is of course conceivable, without departing from the scope of the invention.
- the guide path has a number of turns greater than or equal to two, and even more preferably greater than or equal to four. Nevertheless, other possibilities can be envisaged, such as a single turn, or even an incomplete turn.
- the rotating means take the form of a disk in which is made, in an emergent manner or through, the spiral guide path.
- the invention also relates to an air displacement pipetting system comprising a device for controlling the pipetting and adjustment of a volume to be sampled as described above.
- the pipetting system comprises a piston slidably mounted as well as means for connecting the piston to said connecting member.
- means of the blade type or elastic rod are retained, but any other means can be envisaged, for transmitting the spiral movement of the connecting member moving in the spiral path, in a sliding movement of the piston. during pipetting. It may for example be alternately one or more articulated rods.
- the system is a pipette, manual or motorized, comprising a high portion forming a handle equipped with the rod-shaped control member at the end of which is provided a control button.
- the pipetting system is an automaton.
- FIG. 1 represents a perspective view of an air displacement sampling pipette, according to a preferred embodiment of the present invention
- FIG. 2 represents a schematic sectional view of the pipette shown in FIG. 1;
- FIG. 3 is a perspective view of the pipette control and volume control device to be taken from the pipette shown in the preceding figures;
- FIGS. 5a and 5b show the pipette in different successive states during a pipetting operation
- Figures 6a and 6b are views similar to those of Figures 5a and 5b, with the pipette set to take a different volume of liquid.
- FIG. 1 there is shown a manually operated air displacement sampling pipette 1 according to a preferred embodiment of the present invention.
- the terms “high” and “low” are to be considered with the pipette held vertically, in the pipetting position or close to this same position.
- FIG 1 there is shown the pipette 1 held by the hand 2 of an operator, who, with his thumb 4, actuates the pipette to generate the dispensing of a liquid that has been previously sucked.
- the pipette 1 comprises a handle 6 forming an upper body of the pipette, the handle of which opens a rod-shaped pipetting control member carrying at its upper end, in the pipetting position, a control button 12 whose upper part is intended to undergo the pressure of the thumb of the operator.
- a display screen (not shown) can be provided on the handle 6.
- the volume adjustment means to be taken are also accessible to the user. operator on this handle 6.
- the pipette 1 comprises a removable lower part 14, which ends downwards by a cone holder 16 receiving a consumable 18, also called sampling cone. In known manner, after pipetting, the cone can be ejected mechanically by an ejector 20 whose actuating button 22 is for example projecting on the top of the handle, close to the control knob 12.
- FIG. 2 it is shown schematically the pipette 1, with its device 30 for controlling the pipetting and volume control to be taken, integrated in the handle 6.
- This device 30 specific to the present invention, will be described in detail. below.
- the piston 50 of the pipette which makes it possible to generate the displacement of air necessary for the sampling operations.
- the piston 50 is slidably movable along a vertical axis 26, in a suction chamber 52 defined inside the lower part 14.
- the axis 26 corresponds to the longitudinal axis of the pipette, on which are centered a high number of components, as well as the cone 18.
- the device comprises firstly the rod-shaped control member 10, displaceable in translation relative to the handle 6, along a translation axis 25 parallel to the axis 26. At its lower end, the rod 10 has a rack 10a.
- the device 30 also comprises rotary actuating means 32, rotatably mounted relative to the handle 6 according to a first axis of rotation 34, preferably orthogonal to the axes 25, 26.
- the means 32 have a toothed sector 36 meshing with the rack 10a.
- a translation of the rod 10 about the axis 25 causes a rotation of the means 32 along the axis 34.
- the sector 36 corresponds to a disk sector of about 180 °.
- the means 32 also comprise a finger 38 integral in rotation with the sector 36, the finger being arranged in a plane orthogonal to the first axis of rotation 34 and extending in a direction intercepting the same axis 34.
- the finger 38 is equipped with a straight groove 40 extending in the direction longitudinal of this finger.
- a link member 42 in the form of pins is slidably mounted.
- the pin 42 is intended to be connected to the piston 50 in order to apply to it the up / down movements necessary for the pipetting operations.
- a rod or elastic blade 44 whose upper end is mounted on the pin 42, and whose lower end is mounted on the upper end of the piston 50.
- the device 30 also comprises means for adjusting the volume to be sampled. These are rotary means provided with a worm 54 extended by a shaft 56 at the end of which is an adjustment knob 58 separate from the pipetting control button 12. The adjustment knob 58 is controllable since the Outside of the handle 6. These adjustment means are arranged along an axis 60 orthogonal to each of the axes 34, 26.
- the device 30 comprises rotary means 62 in overall disk shape defining, for the connecting member, a guiding path 64 in the form of a spiral.
- the path 64 then takes the form of a groove or groove opening or through, in which the pin 42 is guided.
- the diameter of the pin is substantially identical to the width of the groove / groove extending in the form of a spiral, preferably an Archimedean spiral.
- the number of turns of the path 64 is for example between 2 and 6.
- This spiral is centered on a second axis of rotation 66, which is preferably substantially coincident with the aforementioned first axis of rotation.
- This axis 66 corresponds to that of the disk 62 which is therefore in a plane parallel to the axes 25, 26.
- the disc 62 has at its periphery 68 a helical toothing (not shown) meshing with the worm 54. As a result, the disc is coupled to the volume adjustment means so that the actuation in rotation of the latter causes a rotation of the disk 62 along the second axis of rotation 66.
- FIGS. 5a and 5b a portion of the pipette 1 is shown in different states during a lowering operation of the piston 50.
- This descent operation is that carried out prior to sampling the liquid, or corresponds to the liquid dispensing operation performed after sampling.
- the operator actuates the control knob 12 to cause the lowering of the rod 10.
- the meshing between the rack 10a and the toothed sector 36 causes the rotation of the means 32 according to FIG. 34.
- this rotation maintained by the descent of the rod 10 simultaneously occurs a relative movement of the pin 42 along the spiral guide path 64 provided on the disk 62 remaining fixed, and a sliding This slide is necessary to cope with the progressive evolution of the diameter of the turn in which the pin 42 is guided during the descent operation.
- the pin 42 moves along the path 64 starting from a high point with respect to the pipette body, towards a low point, by adopting a spiral path. Therefore, during the descent, the pin 42 also deviates from the axis 26 on which it was initially placed with the control rod in the high position, which causes the blade 44 to elastically deform in bending. This blade then drives the downward sliding movement of the piston 50.
- a complete downward stroke of the piston is achieved by a complete downward stroke of the control rod 10, causing the toothed sector 36 to rotate about an angle of about 180 °.
- the pin 42 after having moved away from the axis 26, returns to the lower position by being again arranged on the same axis. During this race, the piece 42 then travels along a half-turn of the spiral 64.
- the equipment is then reassembled in a conventional manner, releasing the control knob 12 and under the effect of return springs (not shown). This rise, operated for example for the withdrawal of liquid, is then followed by a new race downwards for the dispensing of the liquid taken.
- the sliding axis 70 of the pin 42 is substantially coincidental with the axis 26.
- This axis 70 corresponding to in the longitudinal direction of the groove 40, is so arranged that the actuation of the adjusting knob 58 causes the pin 42 to move along a vertical diameter of the spiral, remaining on the axis 26.
- the adjustment shown is obtained thanks to two complete turns of the disk 62 with respect to the maximum volume sampling position shown in FIG. 2.
- a complete stroke of the control rod 10 causes the actuating means 32 to rotate about 180 °, just as in each of all others Volume adjustment positions to be taken.
- the pin 42 thus moves along the path 64 starting from a high point with respect to the pipette body, towards a low point, by adopting a spiral trajectory which distances it from the axis 26 before finding it at low point, as shown in Figure 6b.
- the invention thus advantageously makes it possible to reliably and accurately collect different volumes of liquid, while maintaining a substantially identical pipetting stroke.
- the device 30 described above may have a disengageable appearance, allowing the implementation of a purging operation of the pipette after dispensing.
- various modifications may be made by those skilled in the art to the invention which has just been described, solely by way of non-limiting examples.
- the invention can be applied to a motorized pipette or to an automaton for which the displacement in translation of the control member is effected via a motor.
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1353039A FR3004258B1 (fr) | 2013-04-04 | 2013-04-04 | Systeme de pipetage a commande et reglage de volume ameliores |
PCT/EP2014/056533 WO2014161857A1 (fr) | 2013-04-04 | 2014-04-01 | Systeme de pipetage a commande et reglage de volume ameliores |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2981358A1 true EP2981358A1 (fr) | 2016-02-10 |
EP2981358B1 EP2981358B1 (fr) | 2017-03-15 |
Family
ID=49003832
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14715882.8A Active EP2981358B1 (fr) | 2013-04-04 | 2014-04-01 | Systeme de pipetage a commande et reglage de volume ameliores |
Country Status (11)
Country | Link |
---|---|
US (1) | US9649628B2 (fr) |
EP (1) | EP2981358B1 (fr) |
JP (1) | JP2016518973A (fr) |
KR (1) | KR20150138314A (fr) |
CN (1) | CN105073264B (fr) |
BR (1) | BR112015024965A2 (fr) |
CA (1) | CA2908312A1 (fr) |
ES (1) | ES2628640T3 (fr) |
FR (1) | FR3004258B1 (fr) |
PL (1) | PL2981358T3 (fr) |
WO (1) | WO2014161857A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3019895B1 (fr) | 2014-04-10 | 2016-05-27 | Gilson Sas | Systeme de pipetage multicanaux comprenant deux chambres d'aspiration imbriquees l'une dans l'autre |
FR3040896B1 (fr) * | 2015-09-15 | 2017-10-13 | Gilson Sas | Pipette de prelevement comprenant un organe de commande a double fonction d'ejection de cone et de deverrouillage du systeme de reglage de volume |
FR3049877B1 (fr) * | 2016-04-12 | 2018-04-13 | Gilson Sas | Pipette pour le prelevement d'une plage etendue de volumes de liquide |
CN110057630B (zh) * | 2019-05-05 | 2021-05-18 | 东北大学 | 一种阿基米德螺线式质谱仪连续性变压取样装置及方法 |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE548210A (fr) | 1955-01-14 | |||
US2946486A (en) * | 1957-05-29 | 1960-07-26 | Manostat Corp | Analytical device |
US4098125A (en) * | 1977-03-30 | 1978-07-04 | Lee Thomas E | Adjustable volume pipetting device |
US4096751A (en) * | 1977-06-15 | 1978-06-27 | Oxford Laboratories Inc. | Hand-held micropipettor with fluid transfer volume adjustment mechanism |
US4228831A (en) * | 1978-12-11 | 1980-10-21 | Abbott Laboratories | Probe and syringe drive apparatus |
US4395921A (en) * | 1981-06-26 | 1983-08-02 | Scientific Manufacturing Industries, Inc. | Adjustable volume liquid dispenser |
US4527437A (en) * | 1983-07-06 | 1985-07-09 | Wescor, Inc. | Pipette controller |
GB2174459B (en) * | 1985-05-04 | 1988-05-25 | Jencons | Liquid dispensing means |
JPS6243634U (fr) | 1985-09-04 | 1987-03-16 | ||
US4929428A (en) * | 1988-02-19 | 1990-05-29 | Fuji Photo Film Co., Ltd. | Quantitative pipette |
DE4141608C2 (de) * | 1991-12-17 | 1993-12-02 | Eppendorf Geraetebau Netheler | Pipettiervorrichtung |
FI921648A0 (fi) * | 1992-04-13 | 1992-04-13 | Labsystems Oy | Stegpipett. |
FI961649A0 (fi) * | 1996-04-15 | 1996-04-15 | Labsystems Oy | Spaerrpipett |
FI101456B1 (fi) | 1996-11-13 | 1998-06-30 | Labsystems Oy | Monisylinteripipetti |
US6455006B1 (en) * | 1999-04-20 | 2002-09-24 | Kyoto Electronics Manufacturing Co., Ltd. | Apparatus for aspirating and discharging a sample quantity |
US6428750B1 (en) * | 2000-02-17 | 2002-08-06 | Rainin Instrument, Llc | Volume adjustable manual pipette with quick set volume adjustment |
EP1344565A1 (fr) * | 2002-03-13 | 2003-09-17 | The Automation Partnership (Cambridge) Limited | Distribution de gouttelettes à volume faible |
DE102004003434B4 (de) * | 2004-01-21 | 2006-06-08 | Eppendorf Ag | Pipettiervorrichtung mit einer Verdrängungseinrichtung und einer damit lösbar verbundenen Antriebseinrichtung |
US7988934B2 (en) * | 2006-04-28 | 2011-08-02 | Tecan Trading Ag | Carrier for positioning objects in relation to laboratory articles |
US8029742B2 (en) * | 2008-05-05 | 2011-10-04 | Integra Biosciences Corp. | Multi-channel pipettor with repositionable tips |
CN202113872U (zh) * | 2011-05-13 | 2012-01-18 | 梅方敏 | 一种可调微量移液器 |
CN202427459U (zh) * | 2011-11-10 | 2012-09-12 | 湖北中烟工业有限责任公司 | 一种带吸球和微孔的能准确控制排放量的移液管 |
US9295986B2 (en) * | 2012-05-02 | 2016-03-29 | Eppendorf Ag | Pipette with releasable locking of rotational position of actuating element |
CN102698824A (zh) * | 2012-06-07 | 2012-10-03 | 林昂 | 一种新型移液器 |
-
2013
- 2013-04-04 FR FR1353039A patent/FR3004258B1/fr active Active
-
2014
- 2014-04-01 CA CA2908312A patent/CA2908312A1/fr not_active Abandoned
- 2014-04-01 CN CN201480019394.3A patent/CN105073264B/zh not_active Expired - Fee Related
- 2014-04-01 PL PL14715882T patent/PL2981358T3/pl unknown
- 2014-04-01 US US14/781,569 patent/US9649628B2/en active Active
- 2014-04-01 WO PCT/EP2014/056533 patent/WO2014161857A1/fr active Application Filing
- 2014-04-01 EP EP14715882.8A patent/EP2981358B1/fr active Active
- 2014-04-01 JP JP2016505800A patent/JP2016518973A/ja active Pending
- 2014-04-01 KR KR1020157031182A patent/KR20150138314A/ko not_active Application Discontinuation
- 2014-04-01 ES ES14715882.8T patent/ES2628640T3/es active Active
- 2014-04-01 BR BR112015024965A patent/BR112015024965A2/pt not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP2981358B1 (fr) | 2017-03-15 |
CA2908312A1 (fr) | 2014-10-09 |
WO2014161857A1 (fr) | 2014-10-09 |
PL2981358T3 (pl) | 2017-09-29 |
KR20150138314A (ko) | 2015-12-09 |
JP2016518973A (ja) | 2016-06-30 |
FR3004258A1 (fr) | 2014-10-10 |
FR3004258B1 (fr) | 2015-05-15 |
US9649628B2 (en) | 2017-05-16 |
BR112015024965A2 (pt) | 2017-07-18 |
CN105073264B (zh) | 2017-04-12 |
CN105073264A (zh) | 2015-11-18 |
ES2628640T3 (es) | 2017-08-03 |
US20160059229A1 (en) | 2016-03-03 |
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