US20020045595A1 - N6 heterocyclic 8-modified adenosine derivatives - Google Patents
N6 heterocyclic 8-modified adenosine derivatives Download PDFInfo
- Publication number
- US20020045595A1 US20020045595A1 US09/881,281 US88128101A US2002045595A1 US 20020045595 A1 US20020045595 A1 US 20020045595A1 US 88128101 A US88128101 A US 88128101A US 2002045595 A1 US2002045595 A1 US 2002045595A1
- Authority
- US
- United States
- Prior art keywords
- alkyl
- aryl
- group
- heteroaryl
- hydrogen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- OHSFDRQOQACGHT-UHFFFAOYSA-N CCC1=NC2=C(N=C(C)N=C2NC)N1C1OC(COC)C(OC)C1OC Chemical compound CCC1=NC2=C(N=C(C)N=C2NC)N1C1OC(COC)C(OC)C1OC OHSFDRQOQACGHT-UHFFFAOYSA-N 0.000 description 3
- LUZZJVRAEIKNBV-UHFFFAOYSA-M C#C=N.CN.CNC1=NC=NC2=C1N=C(Cl)N2C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=C(N(C)C)N2C1OC(CO)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=CN2C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=CN2C1OC(CO)C(O)C1O.II.OCC1OC(N2C=NC3=C2N=CN=C3Cl)C(O)C1O.[V]I Chemical compound C#C=N.CN.CNC1=NC=NC2=C1N=C(Cl)N2C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=C(N(C)C)N2C1OC(CO)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=CN2C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CNC1=NC=NC2=C1N=CN2C1OC(CO)C(O)C1O.II.OCC1OC(N2C=NC3=C2N=CN=C3Cl)C(O)C1O.[V]I LUZZJVRAEIKNBV-UHFFFAOYSA-M 0.000 description 1
- LIQKFKLCCLIPHV-UHFFFAOYSA-N C#CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound C#CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O LIQKFKLCCLIPHV-UHFFFAOYSA-N 0.000 description 1
- BARBUDYBVPHDJT-UHFFFAOYSA-N C#C[Na].CCC1=NC2=C(N=CN=C2NC)N1C1OC(CO)C(O)C1O.CNC1=NC=NC2=C1N=C(Cl)N2C1OC(COC(C)=O)C(OC(C)=O)C1OC(C)=O.[V] Chemical compound C#C[Na].CCC1=NC2=C(N=CN=C2NC)N1C1OC(CO)C(O)C1O.CNC1=NC=NC2=C1N=C(Cl)N2C1OC(COC(C)=O)C(OC(C)=O)C1OC(C)=O.[V] BARBUDYBVPHDJT-UHFFFAOYSA-N 0.000 description 1
- QIMUSKQHBONZTM-UHFFFAOYSA-N C.C.CC.CC.CC.CCC Chemical compound C.C.CC.CC.CC.CCC QIMUSKQHBONZTM-UHFFFAOYSA-N 0.000 description 1
- DZQNHLWDANVRLC-UHFFFAOYSA-N C=CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound C=CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O DZQNHLWDANVRLC-UHFFFAOYSA-N 0.000 description 1
- OLFKQTPSBLECBB-UHFFFAOYSA-N CC(C)NC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CC(C)NC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O OLFKQTPSBLECBB-UHFFFAOYSA-N 0.000 description 1
- 0 CC([C@]1*(C)=C)OCC1*(C)=C Chemical compound CC([C@]1*(C)=C)OCC1*(C)=C 0.000 description 1
- QEDFURFYPKTJLS-UHFFFAOYSA-N CC.CC.CC.CCC Chemical compound CC.CC.CC.CCC QEDFURFYPKTJLS-UHFFFAOYSA-N 0.000 description 1
- FUPCHHHJZXZMES-UHFFFAOYSA-N CCCCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CCCCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O FUPCHHHJZXZMES-UHFFFAOYSA-N 0.000 description 1
- OTWPMOAHZCVRQU-UHFFFAOYSA-N CCCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CCCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O OTWPMOAHZCVRQU-UHFFFAOYSA-N 0.000 description 1
- XYSJIMQZGFBNHW-UHFFFAOYSA-N CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CC(=O)OC)C(OC(C)=O)C1OC(C)=O.CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O XYSJIMQZGFBNHW-UHFFFAOYSA-N 0.000 description 1
- ACQYXEQQLQUKCY-UHFFFAOYSA-N CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O ACQYXEQQLQUKCY-UHFFFAOYSA-N 0.000 description 1
- TUHJRCVVDMCYRC-UHFFFAOYSA-N CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O.COC(=O)CC1OC(N2C(Cl)=NC3=C2N=CN=C3NC2CCOC2)C(OC(C)=O)C1OC(C)=O.COC(=O)CC1OC(N2C=NC3=C2N=CN=C3NC2CCOC2)C(OC(C)=O)C1OC(C)=O.NC1CCOC1.OCC1OC(N2C=NC3=C2N=CN=C3Cl)C(O)C1O.OCC1OC(N2C=NC3=C2N=CN=C3NC2CCOC2)C(O)C1O Chemical compound CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O.COC(=O)CC1OC(N2C(Cl)=NC3=C2N=CN=C3NC2CCOC2)C(OC(C)=O)C1OC(C)=O.COC(=O)CC1OC(N2C=NC3=C2N=CN=C3NC2CCOC2)C(OC(C)=O)C1OC(C)=O.NC1CCOC1.OCC1OC(N2C=NC3=C2N=CN=C3Cl)C(O)C1O.OCC1OC(N2C=NC3=C2N=CN=C3NC2CCOC2)C(O)C1O TUHJRCVVDMCYRC-UHFFFAOYSA-N 0.000 description 1
- ZEZCKDOYZJTJEP-UHFFFAOYSA-N CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(COC(C)=O)C(OC(C)=O)C1OC(C)=O Chemical compound CCNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(COC(C)=O)C(OC(C)=O)C1OC(C)=O ZEZCKDOYZJTJEP-UHFFFAOYSA-N 0.000 description 1
- JGDOBJPXLSCHNA-UOQRJXGVSA-N CCNc1nc2c(NC3COCC3)ncnc2[n]1C([C@@H]([C@@H]1OC(C)=O)OC(C)=O)O/C1=C/OC(C)=O Chemical compound CCNc1nc2c(NC3COCC3)ncnc2[n]1C([C@@H]([C@@H]1OC(C)=O)OC(C)=O)O/C1=C/OC(C)=O JGDOBJPXLSCHNA-UOQRJXGVSA-N 0.000 description 1
- PAQCXJMAPZRQPC-UHFFFAOYSA-N CNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound CNC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O PAQCXJMAPZRQPC-UHFFFAOYSA-N 0.000 description 1
- GGJSFMZSKKSAGE-UHFFFAOYSA-N COC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O Chemical compound COC1=NC2=C(N=CN=C2NC2CCOC2)N1C1OC(CO)C(O)C1O GGJSFMZSKKSAGE-UHFFFAOYSA-N 0.000 description 1
- XNIDFKYODVHMCD-UHFFFAOYSA-N OCC1OC(N2C(NCC3=CC=CC=C3)=NC3=C2N=CN=C3NC2CCOC2)C(O)C1O Chemical compound OCC1OC(N2C(NCC3=CC=CC=C3)=NC3=C2N=CN=C3NC2CCOC2)C(O)C1O XNIDFKYODVHMCD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/167—Purine radicals with ribosyl as the saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
- A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
Definitions
- compositions of this invention are selective, partial or full adenosine A 1 receptor agonists, and as such, are useful for modifying cardiac activity, modifying adipocyte function, treating central nervous system disorders, and treating diabetic disorders and obesity in mammals, and especially in humans.
- a 1 and A 2A There are at least two subtypes of adenosine receptors in the heart: A 1 and A 2A . Each subtype affects different physiological functions.
- the A 1 adenosine receptor mediates two distinct physiological responses. Inhibition of the cardiostimulatory effects of catecholamine are mediated via the inhibition of adenylate cyclase, whereas the direct effects to slow the heart rate (HR) and to prolong impulse propagation through the AV node are due in great part to activation of I KAd0 .
- HR heart rate
- a 1 receptor agonists for the treatment of supraventricular tachycardias, including termination of nodal re-entrant tachycardias, and control of ventricular rate during atrial fibrillation and flutter.
- a clinical utility of A 1 agonists therefore is in the treatment of acute and chronic disorders of heart rhythm, especially those diseases characterized by rapid heart rate where the rate is driven by abnormalities in the sinoatrial, atria, and AV nodal tissues.
- disorders include but are not limited to atrial fibrillation, supraventricular tachycardia and atrial flutter.
- Exposure to A 1 agonists causes a reduction in the heart rate and a regularization of the abnormal rhythm thereby improving cardiovascular function.
- a 1 agonists through their ability to inhibit the effects of catecholamines, decrease cellular cAMP, and thus, should have beneficial effects in the failing heart where increased sympathetic tone increases cellular cAMP levels. The latter has been shown to be associated with increased likelihood of ventricular arrhythmias and sudden death. All of the above concepts are discussed in reviews regarding the effects of adenosine on cardiac electrophysiology (see B. Lerman and L. Belardinelli Circulation, Vol. 83 (1991), P 1499-1509 and J. C. Shryock and L. Belardinelli, Am. J. Cardiology, Vol. 79 (1997) P 2-10).
- a controversial area in the field of A 1 adenosine agonism is that the benefit of preconditioning of the heart prior to ischemia may be due to binding of adenosine to the A 1 receptor.
- Evidence for this hypothesis comes from a rabbit ischemia model wherein 2-chloro-N6-cyclopentyladenosine (CCPA) and R-PIA were administered prior to ischemia providing protection with respect to infarct size (J. D. Thornton et al. Circulation Vol. 85 (1992) 659-665).
- NIDDM non-insulin-dependent diabetes mellitus
- a 1 agonist in central nervous disorders has been reviewed and the content are included herein by reference (L. J. S. Knutsen and T. F. Murray In Purinergic Approaches in Experimental Therapeutics, Eds. K. A. Jacobson and M. F. Jarvis (1997) Wiley-Liss, N.Y., P 423-470).
- a mixed A 2A A 1 agonist, metrifudil, has been shown to be a potent anticonvulsant against seizures induced by the inverse benzodiazepine agonist methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate (DMCM, H.
- a second area where an A 1 adenosine agonist has a benefit is in animal models of forebrain ishemia as demonstrated by Knutsen et al (J. Med. Chem. Vol. 42 (1999) p. 3463-3477).
- the benefit in neuroprotection is believed to be in part due to the inhibition of the release of excitatory amino acids (ibid).
- a 1 agonists There are a number of full A 1 agonists disclosed in the prior art. However, the agonists disclosed are generally in the forms that are not useful in the mammalian body. Because useful forms of A 1 agonists may not always be stable, soluble or they may have other properties that make their incorporation into therapeutic dosage forms difficult, it is often necessary to identify compositions that are more easily incorporated into therapeutic dosage forms in order to provide the desired therapeutic effect. Also, these agonists fail as useful therapeutics due to side effects caused by the non-selective stimulation of the A 1 adenosine receptor in all biologically available tissues and the desensitization of the desired response preempting their use as chronic agents. Therefore, there remains a need for specific and selective A 1 agonists, precursors and/or pro-drugs that are converted in the body into useful therapeutic compositions.
- This invention includes heterocyclic 8 modified adenosine derivative compositions that are useful partial or full adenosine A 1 receptor agonists.
- This invention also includes pharmaceutical compositions including one or more heterocyclic 8 modified adenosine derivative compositions.
- this invention includes heterocyclic 8 modified adenosine derivatives having the formula:
- this invention includes methods for administering compositions of this invention to mammals, and especially to humans, to stimulate coronary activity, to modify adipocyte function, to treat central nervous system disorders, and to treat diabetic disorders.
- this invention is pharmaceutical compositions of matter comprising at least one composition of this invention and one or more pharmaceutical excipients.
- This invention includes a class of heterocyclic 8 modified adenosine derivatives having the formula:
- R 1 is a monocyclic or polycyclic heterocyclic group containing from 3 to 15 carbon atoms wherein at least one carbon atom is replaced with an atom or molecule selected from the group consisting of N, O, P and S—(O) 0-2 and wherein R 1 does not contain an epoxide group;
- R 2 is selected from the group consisting of hydrogen, halo, CF 3 , and cyano;
- R 3 , R 4 , and R 5 are independently selected from the group consisting of hydrogen, —(CO)—R′, —(CO)—R′′, and —(CO)—R′′′ wherein R′, R′′, and R′′′ are independently selected from the group consisting of C 1-15 alkyl, C 2-15 alkenyl, C 2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, which alkyl, alkenyl, alkynyl, aryl, heterocyclyl, and heteroaryl are optionally substituted with 1 to 3 substituents independently selected from the group of halo, NO 2 , alkyl, heterocyclyl, aryl, heteroaryl, CF 3 , CN, OR 20 , SR 20 , S(O)R 22 , SO 2 R 22 , SO 2 N(R 20 ) 2 , SO 2 NR 20 COR 22 , SO 2 NR 20 CO 2 R 22 , SO 2 NR 20
- R 6 and R 7 are independently selected from the group consisting of hydrogen, C 1-15 alkyl, C 2-15 alkenyl, C 2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, heterocyclyl and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, NO 2 , heterocyclyl, aryl, heteroaryl, CF 3 , CN, OR 20 , SR 20 , N(R 20 ) 2 , S(O)R 22 , SO 2 R 22 , SO 2 N(R 20 ) 2 , SO 2 NR 20 COR 22 , SO 2 NR 20 CO 2 R 22 , S(O) 3 R 20 , P(O)(OR 20 ) 2 , SO 2 NR 20 CON(R 20 ) 2 , NR 20 COR 22 , NR 20
- R 20 is selected from the group consisting of H, C 1- 15 alkyl, C 2-15 alkenyl, C 2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or aryl or heteroaryl amide, CN, O-C 1-6 alkyl, CF 3 , aryl, and heteroaryl; and
- R 22 is selected from the group consisting of C 1-15 alkyl, C 2-15 alkenyl, C 2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or aryl or heteroaryl amide, CN, O-CI6 alkyl, CF 3 , aryl, and heteroaryl.
- X 1 NR 7 ;
- R 2 is hydrogen;
- R 3 , R 4 , and R 5 are each independently selected from the group consisting of hydrogen, —(CO)—R′, —(CO)—R′′, or —(CO)—R′′′ wherein R′, R′′, and R′′′ are independently selected from the group consisting of C 1-6 alkyl, and preferably methyl;
- R 6 is selected from the group consisting of C 1-3 alkyl and hydrogen with hydrogen being preferred;
- R 7 is independently selected from the group consisting of hydrogen, C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with 1 substituent independently selected from the group consisting of alkyl, aryl, CF 3 , OR 20 , SR 20 , C 2 R 20 , S(O) 3 R 20 , and wherein optional aryl substituent is optional
- X 1 NR 7 ;
- R 2 is hydrogen;
- R 3 , R 4 , R 5 and R 6 are each hydrogen; and
- R 7 is C 1-6 alkyl wherein the alkyl, is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl wherein the optional aryl substituent is further optionally substituted with halo, alkyl, and CF 3 . More preferably, R 7 is C 1-4 alkyl that is optionally substituted with phenyl.
- X 1 NR 7 ;
- R 2 , R 3 , R 4 , R 5 , and R 6 are each hydrogen; and
- R 7 is C 2-4 alkenyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl and aryl. More preferably, R 7 is C 2-3 alkenyl.
- X 1 NR 7 ;
- R 2 , R 3 , R 4 , R 5 , and R 6 are each hydrogen; and
- R 7 is C 2-4 alkynyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl. More preferably, R 7 is C 2-3 alkynyl.
- R 1 is preferably mono or polysubstituted with one or more compounds selected from the group consisting of halogen, oxo, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, cyano and mixtures thereof.
- R 1 is a monocyclic, bicyclic, or tricyclic cycloalkyl group containing from 3 to 15 carbon atoms wherein at least one carbon atom is substituted with an atom or molecule selected from the group consisting of O or S-(O) 0-2 .
- R 1 moiety is:
- R 1 ′, R 1 ′′, R 1 ′′′, and R 1 ′′ 41 may each individually be selected from the group halogen, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, and cyano, and X is O, or S (—O) 0-2 , alternately, R 1 ′′′and R 1 ′′′′ may be a single oxygen atom. More preferably, R 1 ′, R 1 ′′, R 1 ′′′, and R 1 ′′′′ are each individually selected from the group hydrogen, lower alkyl, and substituted lower alkyl.
- R 1 is selected from the group consisting of 3-tetrahydrofuranyl, 3-tetrahydrothiofuranyl 4-pyranyl, and 4 thiopyranyl.
- compositions of this invention include: 2- ⁇ 6-[((3 R)oxolan-3-yl)amino]-8-[(methylethyl)amino]purin-9-yl ⁇ (4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3 ,4-diol; 2- ⁇ 6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-enylamino)purin-9-yl ⁇ (4 S,2 R,3 R,5 R)-5-(hy droxymethyl)oxolane-3,4-diol; 2- ⁇ 6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-ynylamino)purin-9-yl ⁇ (4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2- ⁇ 6-[((3 R)
- Halo or “Halogen”—alone or in combination means all halogens, that is, chloro (Cl), fluoro (F), bromo (Br), iodo (J).
- Haldroxyl refers to the group —OH.
- Thiol or “mercapto” refers to the group —SH.
- Alkyl alone or in combination means an alkane-derived radical containing from 1 to 20, preferably 1 to 15, carbon atoms (unless specifically defined). It is a straight chain alkyl, branched alkyl or cycloalkyl. Preferably, straight or branched alkyl groups containing from 1 -15, more preferably 1 to 8, even more preferably 1-6, yet more preferably 1-4 and most preferably 1-2, carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl and the like.
- the term “lower alkyl” is used herein to describe the straight chain alkyl groups described immediately above.
- cycloalkyl groups are monocyclic, bicyclic or tricyclic ring systems of 3-8, more preferably 3-6, ring members per ring, such as cyclopropyl, cyclopentyl, cyclohexyl, adamantyl and the like.
- Alkyl also includes a straight chain or branched alkyl group that contains or is interrupted by a cycloalkyl portion. The straight chain or branched alkyl group is attached at any available point to produce a stable compound. Examples of this include, but are not limited to, 4-(isopropyl)-cyclohexylethyl or 2-methyl-cyclopropylpentyl.
- a substituted alkyl is a straight chain alkyl, branched alkyl, or cycloalkyl group defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbon
- Alkenyl alone or in combination means a straight, branched, or cyclic hydrocarbon containing 2-20, preferably 2-17, more preferably 2-10, even more preferably 2-8, most preferably 2-4, carbon atoms and at least one, preferably 1-3, more preferably 1-2, most preferably one, carbon to carbon double bond.
- a cycloalkyl group conjugation of more than one carbon to carbon double bond is not such as to confer aromaticity to the ring.
- Carbon to carbon double bonds may be either contained within a cycloalkyl portion, with the exception of cyclopropyl, or within a straight chain or branched portion.
- alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, cyclohexenyl, cyclohexenylalkyl and the like.
- a substituted alkenyl is the straight chain alkenyl, branched alkenyl or cycloalkenyl group defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups,
- Alkynyl alone or in combination means a straight or branched hydrocarbon containing 2-20, preferably 2-17, more preferably 2-10, even more preferably 2-8, most preferably 2-4, carbon atoms containing at least one, preferably one, carbon to carbon triple bond.
- alkynyl groups include ethynyl, propynyl, butynyl and the like.
- a substituted alkynyl refers to the straight chain alkynyl or branched alkenyl defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di- substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di- substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, or the like attached
- R is lower alkyl, or substituted lower alkyl
- R′, R′′′, R′′′′ may independently be hydrogen, halogen, lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined below.
- Alkyl alkynyl refers to a groups —RC ⁇ CR′ where R is lower alkyl or substituted lower alkyl, R′ is hydrogen, lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined below.
- Alkoxy denotes the group —OR, where R is lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, aralkyl, substituted aralkyl, heteroalkyl, heteroarylalkyl, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, or substituted cycloheteroalkyl as defined.
- Acyl denotes groups —C(O)R, where R is hydrogen, lower alkyl substituted lower alkyl, aryl, substituted aryl and the like as defined herein.
- Aryloxy denotes groups —OAr, where Ar is an aryl, substituted aryl, heteroaryl, or substituted heteroaryl group as defined herein.
- Amino denotes the group NRR′, where R and R′ may independently by hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined herein or acyl.
- “Amido” denotes the group —C(O)NRR′, where R and R′ may independently by hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, substituted hetaryl as defined herein.
- Carboxyl denotes the group —C(O)OR, where R is hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, and substituted hetaryl as defined herein.
- Aryl alone or in combination means phenyl or naphthyl optionally carbocyclic fused with a cycloalkyl of preferably 5-7, more preferably 5-6, ring members and/or optionally substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfon
- Substituted aryl refers to aryl optionally substituted with one or more functional groups, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- functional groups e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Heterocycle refers to a saturated, unsaturated, or aromatic carbocyclic group having a single ring (e.g., morpholino, pyridyl or furyl) or multiple condensed rings (e.g., naphthpyridyl, quinoxalyl, quinolinyl, indolizinyl or benzo[b]thienyl) and having at least one hetero atom, such as N, O or S, within the ring, which can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- a single ring e.g., morpholino, pyridy
- Heteroaryl alone or in combination means a monocyclic aromatic ring structure containing 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing one or more, preferably 1-4, more preferably 1-3, even more preferably 1-2, heteroatoms independently selected from the group O, S, and N, and optionally substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroary
- Heteroaryl is also intended to include oxidized S or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen.
- a carbon or nitrogen atom is the point of attachment of the heteroaryl ring structure such that a stable aromatic ring is retained.
- heteroaryl groups are pyridinyl, pyridazinyl, pyrazinyl, quinazolinyl, purinyl, indolyl, quinolinyl, pyrimidinyl, pyrrolyl, oxazolyl, thiazolyl, thienyl, isoxazolyl, oxathiadiazolyl, isothiazolyl, tetrazolyl, imidazolyl, triazinyl, faranyl, benzofuryl, indolyl and the like.
- a substituted heteroaryl contains a substituent attached at an available carbon or nitrogen to produce a stable compound.
- Heterocyclyl alone or in combination means a non-aromatic cycloalkyl group having from 5 to 10 atoms in which from 1 to 3 carbon atoms in the ring are replaced by heteroatoms of O, S or N, and are optionally benzo fused or fused heteroaryl of 5-6 ring members and/or are optionally substituted as in the case of cycloalkyl.
- Heterocycyl is also intended to include oxidized S or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen. The point of attaclunent is at a carbon or nitrogen atom.
- heterocyclyl groups are tetrahydrofuranyl, dihydropyridinyl, piperidinyl, pyrrolidinyl, piperazinyl, dihydrobenzofuryl, dihydroindolyl, and the like.
- a substituted hetercyclyl contains a substituent nitrogen attached at an available carbon or nitrogen to produce a stable compound.
- Substituted heteroaryl refers to a heterocycle optionally mono or poly substituted with one or more functional groups, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- functional groups e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Aralkyl refers to the group —R—Ar where Ar is an aryl group and R is lower alkyl or substituted lower alkyl group.
- Aryl groups can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Heteroalkyl refers to the group —R—Het where Het is a heterocycle group and R is a lower alkyl group. Heteroalkyl groups can optionally be unsubstituted or substituted with e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Heteroarylalkyl refers to the group —R—HetAr where HetAr is an heteroaryl group and R lower alkyl or substituted lower alkyl.
- Heteroarylalkyl groups can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, substituted lower alkyl, alkoxy, alkylthio, acetylene, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Cycloalkyl refers to a divalent cyclic or polycyclic alkyl group containing 3 to 15 carbon atoms.
- Substituted cycloalkyl refers to a cycloalkyl group comprising one or more substituents with, e.g., halogen, lower alkyl, substituted lower alkyl, alkoxy, alkylthio, acetylene, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Cycloheteroalkyl refers to a cycloalkyl group wherein one or more of the ring carbon atoms is replaced with a heteroatom (e.g., N, O, S or P).
- Substituted cycloheteroalkyl refers to a cycloheteroalkyl group as herein defined which contains one or more substituents, such as halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- substituents such as halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Alkyl cycloalkyl denotes the group —R-cycloalkyl where cycloalkyl is a cycloalkyl group and R is a lower alkyl or substituted lower alkyl.
- Cycloalkyl groups can optionally be unsubstituted or substituted with e.g. halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- Alkyl cycloheteroalkyl denotes the group —R-cycloheteroalkyl where R is a lower alkyl or substituted lower alkyl.
- Cycloheteroalkyl groups can optionally be unsubstituted or substituted with e.g. halogen, lower alkyl, lower alkoxy, alkylthio, amino, amido, carboxyl, acetylene, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- the compounds of this invention can be prepared as outlined in Schemes 1-4, below.
- Compounds having the general formula IV can be prepared as shown in Scheme 1.
- Compound I can be prepared through reaction of the corresponding primary amino compound, R 1 NH 2 , through heating with commercially available 6-chloroadenosine in the appropriate solvent (eg. n-butanol, dimethylformamide, and ethanol).
- the primary amino compound, R 1 NH 2 is either commercially available or can be prepared as previously described in U.S. Pat. No. 5,789,416, the specification of which is incorporated herein by reference.
- the pro-drug esters of this invention can be prepared using all of the known methods for ester formation which are included by reference (see Jerry March Organic synthesis and Richard Larock—Methods of Organic Synthesis), and more preferably by those outlined in this application.
- the key intermediate compound III can be prepared by the direct chlorination of the 2′,3′,5′-tri-O-acetyl-N 6 substitued adenosine (II).
- Compound II can be obtained by substitution of 6-chloropurine riboside with an amine (Fleysher, M. H. J. Med. Chem. 1972, 15, 187-191) followed by acetylation of the formed N 6 substituted adenosine (compound I).
- Nucleophilic displacement of the chlorine atom of compound III with different alkyl amines results in the formation of C-8 substituted compounds with simultaneous deacetylation to yield compound IV (Harlof Roelenet al. J. Med. Chem. 1996, 39, 1463-1471).
- Compounds with the general structure V can be prepared by the reaction of compound III or compound I (scheme 1) with sodium aryloxide, alkoxide, arylthiolate or alkylthiolate in alcohol or DMF at room temperature or reflux conditions (G. Buenger and V. Nair, Synthesis, 1990, p 962-966).
- Compound 6 can be obtained by the direct acetylation of compound 5 (Scheme 4).
- This invention also includes pro-drugs of the A 1 agonist compositions of this invention.
- a pro-drug is a drug which has been chemically modified and may be biologically inactive at its site of action, but which will be degraded or modified by one or more enzymatic or in vivo processes to the bioactive form.
- the pro-drugs of this invention should have a different pharmacokinetic profile to the parent enabling improved absorption across the mucosal epithelium, better salt formulation and/or solubility and improved systemic stability.
- the compounds of this invention may be preferably modified at one or more of the hydroxyl groups to form pro-drugs.
- the modifications may be (1) ester or carbamate derivatives which may be cleaved by esterases or lipases, for example; (2) peptides which may be recognized by specific or non specific proteinase; or (3) derivatives that accumulate at a site of action through membrane selection or a pro-drug form or modified pro-drug form, or any combination of (1) to (3) above.
- a compound of this invention contains a basic group
- acid addition salts of the compounds are prepared in a standard manner in a suitable solvent from the parent compound and an excess of acid, such as hydrochloric, hydrobromic, sulfuric, phosphoric, acetic, maleic, succinic, or methanesulfonic.
- the hydrochloric salt form is especially useful.
- a compound of this invention contains an acidic group
- corresponding cationic salts may be prepared.
- the parent compound is treated with an excess of an alkaline reagent, such as hydroxide, carbonate or alkoxide, containing the appropriate cation.
- Cations such as Na + , K + , Ca +2 and NH 4 + are examples of cations present in pharmaceutically acceptable salts.
- Certain of the compounds form inner salts or zwitterions which may also be acceptable.
- compositions of this invention are useful for treating a variety of mammalian disorders and preferably human disorders that are mediated by an A 1 adenosine receptor.
- the compositions of this invention are useful for modifying cardiac activity in mammals experiencing a coronary electrical disorder that can be treated by stimulating an A 1 adenosine receptor.
- coronary electrical disorders that can be treated by the compositions of this invention include supraventricular tachycardias, atrial fibrillation, atrial flutter, and AV nodal re-entrant tachycardia.
- orally active A 1 agonists of this invention that demonstrate an excellent safety profile in treating supraventricular arrhythmias may also be used as a prophylactic for those at high risk of a myocardial ischemia.
- compositions of this invention are also useful for modifying adipocyte function by stimulating an A 1 adenosine receptor that leads to diminished release of NEFA and increased release of leptin.
- Disease states related to adipocyte function that can be modified using compositions of this invention include diabetes, and obesity.
- a 1 AdoR agonists mediate a synergistic stimulation of glucose uptake and transport by insulin (Vergauwen, L. et al, J Clin. Invest. 1994, 93, 974-81; Challiss, R. A. et al, Eur. J. Pharacol., 1992, 226, 121-8).
- Another therapeutic utility of compositions of this invention is more efficient regulation of glucose and a decrease of circulating levels of insulin in patients afflicted with diabetes.
- R-PIA The A 1 receptor agonist, R-PIA, has been shown to increase the leptin released from white adipocytes and augment insulin-stimulated leptin production (M. Ozeck Master's Thesis Univ. of Florida 1999 with L. Belardinelli). Evidence suggests that catecholamines inhibit the production of leptin from adipocytes through activation of P-adrenergic receptors. The anti- ⁇ -adrenergic effects of A 1 agonists on the adipocytes are believed to play a role in the increased release of leptin. The functional role of leptin is multifaceted including decreased appetite, stimulated energy utilization, and increased fertility.
- compositions of this invention may also be used to provide central nervous system neuroprotection by stimulating an A 1 adenosine receptor.
- Central nervous system disorders that may be treated using the compositions of this invention include epilepsy, and stroke.
- compositions of this invention are selective antagonism of the A 1 AdoR in the kidney to inhibit sodium retention, inhibit the exchange of sodium for potassium, and preserve kidney glomerular filtration rate when sodium excretion rises to yield a potassium sparring diuretic that preserves renal function.
- compositions of this invention are further useful for providing cardiomyocyte protection from ischemic events by stimulating an A 1 adenosine receptor.
- Ischemic events treatable using the compositions of this invention include stable angina, unstable angina, cardiac transplant, and myocardial infarction.
- each compound has an intrinsic efficacy associated with it (for a discussion see T. P. Kenakin Stimulus Response Mechanisms. In Pharmacological Analysis of Drug-Receptor Interaction, Ed. Kenakin, T. P. New York: Raven Press, p 39-68).
- This intrinsic efficacy is not defined by it's affinity for the receptor, but it is defined as the quantitative effect of the compound to activate a given effector system (eg. cAMP production) in a given cell type.
- the intrinsic efficacy of a given compound may vary from cell type to cell type and/or from effector system to effector system. When a compound has an intrinsic efficacy lower than a full agonist (i.e.
- a partial agonist is a molecule that binds to a receptor and elicits a response that is smaller than that of a full agonist (submaximal), but also competitively antagonizes the response(s) elicited by a full agonist.
- the tonic action of adenosine with resepct to kidney function is a prime example where a partial A 1 agonist be expected to act as antagonists (e.g. adenosine).
- the tonic action of adenosine with respect to kidney function is a prime example where a partial A 1 agonist could be expected to act as an antagonist.
- the compounds of this invention are believed to have therapeutically useful affinities for the adenosine A 1 receptor, and they will have a range of intrinsic efficacies from full agonist to partial agonist. That is, some compounds may have no effect with respect to a given effector system in a given cell type, but be a full agonist in another cell type and/or effector system.
- the reason for such variable pharmacological behavior relates to the magnitude of the receptor reserve for the A 1 adenosine receptor in any given cell type (eg. AV nodal cells vs. adipocytes) and for a given response.
- the receptor reserve (spare receptor capacity) is the total number of receptors minus the fraction of receptors that is required to induce the maximal response using a full agonist (L. E. Limbird, Cell Surface Receptors: A Short Course on Theory and Methods, Kluwer Acad. Pub. 1996, Boston, Mass.). Therefore, the agonist could be a full agonist at eliciting a response, and a partial agonist for eliciting another response in other tissue or cells and still be an antagonist or lack activity for a third response in another tissue or cell. Consequently, a partial agonist targeted to a selected target is likely to cause fewer side effects than a full agonist.
- a full agonist elicits all the effects mediated by the respective receptor, whereas this is not necessarily the case of a partial agonist.
- the compounds of this invention based on their affinity for the A 1 receptor and their potency and selectivity to elicit A 1 receptor mediated responses have the potential for therapeutic intervention in the multiple disease states described above.
- Partial A 1 agonists may have an added benefit for chronic therapy because they will be less likely to induce desensitization of the A 1 receptor (R. B. Clark, B. J. Knoll, R. Barber TiPS, Vol. 20 (1999) p. 279-286) and to cause side effects.
- Chronic administration of a full agonist (R-N6-phenylisopropyladenosine, R-PIA) for 7 days led to a desensitization of the A 1 receptor in terms of the dromotropic response in guinea pigs (note: a decrease in receptor number was observed - D. M. Dennis, J. C. Shryock, L. Belardinelli JPET, Vol. 272 (1995) p.
- compositions of this invention may be administered orally, intravenously, through the epidermis, bolus, nasally, by inhalation or by any other means known in the art for administering a therapeutic agents.
- the method of treatment comprises the administration of an effective quantity of the chosen compound, preferably dispersed in a pharmaceutical carrier. Dosage units of the active ingredient are generally selected from the range of 0.01 to 100 mg/kg, but will be readily determined by one skilled in the art depending upon the route of administration, age and condition of the patient.
- compositions including the compounds of this invention, and/or derivatives thereof may be formulated as solutions or lyophilized powders for parenteral administration. Powders may be reconstituted by addition of a suitable diluent or other pharmaceutically acceptable carrier prior to use. If used in liquid form the compositions of this invention are preferably incorporated into a buffered, isotonic, aqueous solution. Examples of suitable diluents are normal isotonic saline solution, standard 5% dextrose in water and buffered sodium or ammonium acetate solution. Such liquid formulations are suitable for parenteral administration, but may also be used for oral administration.
- compositions including compounds of this invention may be desirable to add excipients such as polyvinylpyrrolidinone, gelatin, hydroxycellulose, acacia, polyethylene glycol, mannitol, sodium chloride, sodium citrate or any other excipient known to one of skill in the art to pharmaceutical compositions including compounds of this invention.
- the pharmaceutical compounds may be encapsulated, tableted or prepared in an emulsion or syrup for oral administration.
- Pharmaceutically acceptable solid or liquid carriers may be added to enhance or stabilize the composition, or to facilitate preparation of the composition.
- Liquid carriers include syrup, peanut oil, olive oil, glycerin, saline, alcohols and water.
- Solid carriers include starch, lactose, calcium sulfate, dihydrate, teffa alba, magnesium stearate or stearic acid, talc, pectin, acacia, agar or gelatin.
- the carrier may also include a sustained release material such as glycerol monostearate or glycerol distearate, alone or with a wax.
- the amount of solid carrier varies but, preferably, will be between about 20 mg to about 1 gram per dosage unit.
- the pharmaceutical dosages are made using conventional techniques such as milling, mixing, granulation, and compressing, when necessary, for tablet forms; or milling, mixing and filling for hard gelatin capsule forms.
- a liquid carrier When a liquid carrier is used, the preparation will be in the form of a syrup, elixir, emulsion or an aqueous or non-aqueous suspension. Such a liquid formulation may be administered directly or filled into a soft gelatin capsule.
- Compound 8 was prepared as described in example 1 substituting n-propylamine for ethylamine: 1 H NMR (CDCl 3 ) ⁇ 0.85 (t, 3 H), 1.50-1.60( ⁇ , 2 H), 1.80-1.90(m, 1 H), 2.20-3.20 (t, 2 H), 3.60-3.70 (m, 2 H), 3.70-4.00 (m,4 H), 4.05-4.10 (m, 1 H), 4.10-4.15 (m, 1 H), 4.50-4.60 (m, 2 H), 5.75 (d, 1 H), 6.50-6.60(m, 1 H), 7.95 (s, 1 H).
- DDT cells hamster vas deferens smooth muscle cell line
- DMEM Dulbecco's Modified Eagle's Medium
- HBSS Hank's Balanced Salt Solution
- the final pellet was resuspended in 50 mM Tris-HCl pH 7.4 containing 5 mM MgCl 2 , 100 mM NaCl and 1 mM dithiothreitol. This membrane suspension was then placed in liquid nitrogen for 10 min, thawed and used for assays. The protein content was determined with a BradfordTM Assay Kit using bovine serum albumin as standard.
- DMSO serially diluted DMSO stock solution of the compounds of this invention at concentrations ranging from 100 microM to 10 nM.
- the control received 2 microL of DMSO alone, then the antagonist [ 3 H] 8-cyclopentylxanthine (CPX) for pig striatum or the agonist [3H] 2-chloro-6-cyclopentyladenosine (CCPA) for DDT 1 membranes in Tris buffer (50 mM, pH of 7.4) was added to achieve a final concentration of 2 nM. After bation at 23 C. for 2 h, then the solutions were filtered using a membrane harvester using iple washing of the membranes (3 x).
- CPX 8-cyclopentylxanthine
- CCPA 2-chloro-6-cyclopentyladenosine
- a 1 -agonist stimulated [ 35 S] GTP ⁇ S binding was determined by a modification of the method described by Giersckik et al. (1991) and Lorenzen et al. (1993).
- Membrane protein (30-50 ⁇ g) was incubated in a volume of 0.1 ml containing 50 mM Tris-HCl buffer pH 7.4, 5 mM MgCl 2 , 100 mM NaCl, 1 mM dithiothreitol, 0.2 units ml ⁇ 1 adenosine deaminase, 0.5% BSA, 1 mM EDTA, 10 mM GDP, 0.3 nM [ 35 S]GTP ⁇ S and with or without varying concentrations of CPA for 90 min at 30° C.
- Nonspecific binding was determined by the addition of 10 ⁇ M GTP ⁇ S.
- Agonist stimulated binding was determined as the difference between total binding in the presence of CPA and basal binding determined in the absence of CPA.
- Previous reports have shown that agonist stimulated [ 35 S]GTP ⁇ S binding was dependent on the presence of GDP (Gierschik et al., 1991; Lorenzen et al., 1993; Traynor & Nahorski, 1995). In preliminary experiments, it was found that 10 ⁇ M GDP gave the optimal stimulation of CPA dependent [ 35 S]GTP ⁇ S binding and this concentration was therefore used in all studies. In saturation experiments, 0.5 nM [ 35 S]GTP ⁇ S was incubated with 0.5-1000 nM GTP ⁇ S.
- a scintillation proximity assay using rabbit antibodies directed at cAMP using an added tracer of adenosine 3′,5′-cyclic phosphoric acid 2′-O-succinyl-3-[ 125 I] iodotyrosine methyl ester and fluoromicrospheres containing anti-rabbit specific antibodies as described by Amersham Pharmacia Biotech (Biotrak cellular communication assays). Briefly, DDT 1 cells were cultured in clear bottomed 96 well microtiter plates with opaque wells at concentrations between 10 4 to 10 6 cells per well in 40 ⁇ l of HBSS at 37° C. (5% CO 2 and 95% humidity).
- the partial or full A 1 agonists (5 ⁇ l )of this invention were incubated at various concentrations with the DDT 1 cells in the presence of rolipram (50 ⁇ M), and 5 ⁇ M forskolin for 10 min at 37° C.
- the cells were immediately lysed by treatment 5 ⁇ l of 10% dodecyltrimethylammonium bromide followed by shaking using microplate shaker. After incubation of the plate for 5 minutes, an immunoreagent solution (150 ⁇ l containing equal volumes of tracer, antiserum, and SPA fluorospheres) was added to each well followed by sealing the plate.
Abstract
N6 heterocyclic 8 modified adenosine derivatives that are selective, partial or full adenosine A1 receptor partial or full agonists, and as such, are useful for modifying cardiac activity, modifying adipocyte function, treating central nervous system disorders, and treating diabetic disorders and obesity in mammals, and especially in humans.
Description
- 1. Field of the Invention
- There is provided useful drugs and pro-drugs that are N6 heterocyclic 8 modified adenosine derivatives. The compositions of this invention are selective, partial or full adenosine A1 receptor agonists, and as such, are useful for modifying cardiac activity, modifying adipocyte function, treating central nervous system disorders, and treating diabetic disorders and obesity in mammals, and especially in humans.
- 2. Description of the Art
- There are at least two subtypes of adenosine receptors in the heart: A1 and A2A. Each subtype affects different physiological functions. The A1 adenosine receptor mediates two distinct physiological responses. Inhibition of the cardiostimulatory effects of catecholamine are mediated via the inhibition of adenylate cyclase, whereas the direct effects to slow the heart rate (HR) and to prolong impulse propagation through the AV node are due in great part to activation of IKAd0. (B. Lerman and L. Belardinelli Circulation, Vol. 83 (1991), P 1499-1509 and J. C. Shryock and L. Belardinelli The Am. J. Cardiology, Vol. 79 (1997) P 2-10). Both, the anti-β-adrenergic action and direct depressant effects on SA and AV nodal function are mediated by the A1 receptor; there is no role for the A2A receptor in this response to adenosine. A2A receptors mediate the coronary vasodilatation caused by adenosine. Stimulation of the A1 adenosine receptor accordingly shortens the duration and decreases the amplitude of the action potential of AV nodal cells, and hence prolongs the refractory period of the AV nodal cell. The consequence of these effects is to limit the number of impulses conducted from the atria to the ventricles. This forms the basis of the clinical utility of A1 receptor agonists for the treatment of supraventricular tachycardias, including termination of nodal re-entrant tachycardias, and control of ventricular rate during atrial fibrillation and flutter.
- A clinical utility of A1 agonists therefore is in the treatment of acute and chronic disorders of heart rhythm, especially those diseases characterized by rapid heart rate where the rate is driven by abnormalities in the sinoatrial, atria, and AV nodal tissues. Such disorders include but are not limited to atrial fibrillation, supraventricular tachycardia and atrial flutter. Exposure to A1 agonists causes a reduction in the heart rate and a regularization of the abnormal rhythm thereby improving cardiovascular function.
- A1 agonists, through their ability to inhibit the effects of catecholamines, decrease cellular cAMP, and thus, should have beneficial effects in the failing heart where increased sympathetic tone increases cellular cAMP levels. The latter has been shown to be associated with increased likelihood of ventricular arrhythmias and sudden death. All of the above concepts are discussed in reviews regarding the effects of adenosine on cardiac electrophysiology (see B. Lerman and L. Belardinelli Circulation, Vol. 83 (1991), P 1499-1509 and J. C. Shryock and L. Belardinelli, Am. J. Cardiology, Vol. 79 (1997) P 2-10).
- A controversial area in the field of A1 adenosine agonism is that the benefit of preconditioning of the heart prior to ischemia may be due to binding of adenosine to the A1 receptor. Evidence for this hypothesis comes from a rabbit ischemia model wherein 2-chloro-N6-cyclopentyladenosine (CCPA) and R-PIA were administered prior to ischemia providing protection with respect to infarct size (J. D. Thornton et al. Circulation Vol. 85 (1992) 659-665). A1 agonists, as a result of their inhibitory action on cyclic AMP generation, have antilipolytic effects in adipocytes that leads to a decreased release of nonesterified fatty acids (NEFA) (E. A. van Schaick et al J. Pharmacokinetics and Biopharmaceutics, Vol. 25 (1997) p 673-694 and P. Strong Clinical Science Vol. 84 (1993) p. 663-669). Non-insulin-dependent diabetes mellitus (NIDDM) is characterized by an insulin resistance that results in hyperglycemia. Factors contributing to the observed hyperglycemia are a lack of normal glucose uptake and activation of skeletal muscle glycogen synthase (GS). Elevated levels of NEFA have been shown to inhibit insulin-stimulated glucose uptake and glycogen synthesis ( D. Thiebaud et al Metab. Clin. Exp. Vol. 31 (1982) p 1128-1136 and G. Boden et al J. Clin. Invest. Vol. 93 (1994) p 2438-2446). The hypothesis of a glucose fatty acid cycle was proposed by P. J. Randle as early as 1963 (P. J. Randle et al Lancet (1963) p. 785-789). A tenet of this hypothesis would be that limiting the supply of fatty acids to the peripheral tissues should promote carbohydrate utilization (P. Strong et al Clinical Science Vol. 84 (1993) p. 663-669).
- The benefit of an A1 agonist in central nervous disorders has been reviewed and the content are included herein by reference (L. J. S. Knutsen and T. F. Murray In Purinergic Approaches in Experimental Therapeutics, Eds. K. A. Jacobson and M. F. Jarvis (1997) Wiley-Liss, N.Y., P 423-470). Briefly, based on experimental models of epilepsy, a mixed A2A: A1 agonist, metrifudil, has been shown to be a potent anticonvulsant against seizures induced by the inverse benzodiazepine agonist methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate (DMCM, H. Klitgaard Eur. J. Pharmacol. (1993) Vol. 224 p. 221-228). In other studies using CGS 21680, an A2A agonist, it was concluded that the anticonvulsant activity was attributed to activation of the A1 receptor (G. Zhang et al. Eur. J. Pharmacol. Vol. 255 (1994) p. 239-243). Furthermore, A1 adenosine selective agonists have been shown to have anticonvulsant activity in the DMCM model (L. J. S. Knutsen In Adenosine and Adenne Nucleotides: From Molecular Biology to Integrative Physiology; eds. L. Belardinelli and A. Pelleg, Kluwer: Boston, 1995, pp 479-487). A second area where an A1 adenosine agonist has a benefit is in animal models of forebrain ishemia as demonstrated by Knutsen et al (J. Med. Chem. Vol. 42 (1999) p. 3463-3477). The benefit in neuroprotection is believed to be in part due to the inhibition of the release of excitatory amino acids (ibid).
- There are a number of full A1 agonists disclosed in the prior art. However, the agonists disclosed are generally in the forms that are not useful in the mammalian body. Because useful forms of A1 agonists may not always be stable, soluble or they may have other properties that make their incorporation into therapeutic dosage forms difficult, it is often necessary to identify compositions that are more easily incorporated into therapeutic dosage forms in order to provide the desired therapeutic effect. Also, these agonists fail as useful therapeutics due to side effects caused by the non-selective stimulation of the A1 adenosine receptor in all biologically available tissues and the desensitization of the desired response preempting their use as chronic agents. Therefore, there remains a need for specific and selective A1 agonists, precursors and/or pro-drugs that are converted in the body into useful therapeutic compositions.
- This invention includes heterocyclic8 modified adenosine derivative compositions that are useful partial or full adenosine A1 receptor agonists.
- This invention also includes pharmaceutical compositions including one or more heterocyclic8 modified adenosine derivative compositions.
-
- In yet another embodiment, this invention includes methods for administering compositions of this invention to mammals, and especially to humans, to stimulate coronary activity, to modify adipocyte function, to treat central nervous system disorders, and to treat diabetic disorders.
- In a further embodiment, this invention is pharmaceutical compositions of matter comprising at least one composition of this invention and one or more pharmaceutical excipients.
-
- wherein X1=O, S, NR7;
- R1 is a monocyclic or polycyclic heterocyclic group containing from 3 to 15 carbon atoms wherein at least one carbon atom is replaced with an atom or molecule selected from the group consisting of N, O, P and S—(O)0-2 and wherein R1 does not contain an epoxide group;
- Wherein R2 is selected from the group consisting of hydrogen, halo, CF3, and cyano;
- Wherein R3, R4, and R5 are independently selected from the group consisting of hydrogen, —(CO)—R′, —(CO)—R″, and —(CO)—R″′ wherein R′, R″, and R′″ are independently selected from the group consisting of C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, which alkyl, alkenyl, alkynyl, aryl, heterocyclyl, and heteroaryl are optionally substituted with 1 to 3 substituents independently selected from the group of halo, NO2, alkyl, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, SR20, S(O)R22, SO2R22, SO2N(R20)2, SO2NR20COR22, SO2NR20CO2R22, SO2NR20CON(R20)2, NR20COR22, NR20CO2R22, NR20 CON(R 20)2, NR20C(NR20)NHR23, COR20, CO2R20, CON(R20)2, CONR20SO2R22, NR20SO2R22, SO2NR20CO2R22, OCONR20SO2R22, OC(O)R20, C(O)OCH2OC(O)R20, and OCON(R20)2 and wherein each optional heteroaryl, aryl, alkyl, and heterocyclyl substituent is optionally further substituted with halo, NO2, alkyl, CF3, amino, mono- or di- alkylamino, alkyl or aryl or heteroaryl amide, NR20COR22, NR20SO2R22, COR20, CO2R20, CON(R20)2, NR20CON(R20)2, OC(O)R20, OC(O)N(R20)2,SR20, S(O)R22, SO2R22, SO2N(R20)2, CN, or OR20;
- R6 and R7 are independently selected from the group consisting of hydrogen, C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, heterocyclyl and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, NO2, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, SR20, N(R20)2, S(O)R22, SO2R22, SO2N(R20)2, SO2NR20COR22, SO2NR 20CO2R22, S(O)3R20, P(O)(OR20)2, SO2NR20CON(R20)2, NR20COR22, NR20CO2R22, NR2° CON(R20) NR2° C(NR20)NHR2, COR20, C02R2′, CON(R2′)2, CONR 2S02R22, NR20So2R22, SO2NR20CO2R22, OCONR20SO2R22, OC(O)R20, C(O)OCH2OC(O)R20, and OCON(R20)2 and wherein each optional heteroaryl, aryl, and heterocyclyl substituent is optionally further substituted with halo, NO2, alkyl, CF3, amino, mono- or di- alkylamino, alkyl or aryl or heteroaryl amide, NR20COR22, NR20SO2R22, COR20, CO2R20, CON(R20)2, NR20CON(R20)2, OC(O)R20, OC(O)N(R20)2, SR20, S(O)3R20, P(O)(OR20)2, S(O)R22, SO2R22, SO2N(R20)2,CN, or OR20;
- R20 is selected from the group consisting of H, C1- 15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or aryl or heteroaryl amide, CN, O-C1-6 alkyl, CF3, aryl, and heteroaryl; and
- R22 is selected from the group consisting of C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or aryl or heteroaryl amide, CN, O-CI6 alkyl, CF3, aryl, and heteroaryl.
- In more preferred compositions, X1=NR7; R2 is hydrogen; R3, R4, and R5 are each independently selected from the group consisting of hydrogen, —(CO)—R′, —(CO)—R″, or —(CO)—R′″ wherein R′, R″, and R′″ are independently selected from the group consisting of C1-6 alkyl, and preferably methyl; R6 is selected from the group consisting of C1-3 alkyl and hydrogen with hydrogen being preferred; R7 is independently selected from the group consisting of hydrogen, C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with 1 substituent independently selected from the group consisting of alkyl, aryl, CF3, OR20, SR20, C2R20, S(O)3R20, and wherein optional aryl substituent is optionally further substituted with halo, alkyl, CF3; and R20 is selected from the group consisting of H, C1-6 alkyl.
- In another preferred group of compositions, X1=NR7; R2 is hydrogen; R3 , R4, R5 and R6 are each hydrogen; and R7 is C1-6 alkyl wherein the alkyl, is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl wherein the optional aryl substituent is further optionally substituted with halo, alkyl, and CF3. More preferably, R7 is C1-4 alkyl that is optionally substituted with phenyl.
- In another preferred class of compositions, X1=NR7; R2, R3, R4, R5, and R6 are each hydrogen; and R7 is C2-4 alkenyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl and aryl. More preferably, R7 is C2-3 alkenyl.
- In yet another preferred class of compositions, X1=NR7; R2, R3, R4, R5, and R6 are each hydrogen; and R7 is C2-4 alkynyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl. More preferably, R7 is C2-3 alkynyl.
- In the compositions of this invention, R1 is preferably mono or polysubstituted with one or more compounds selected from the group consisting of halogen, oxo, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, cyano and mixtures thereof. More preferably, R1 is a monocyclic, bicyclic, or tricyclic cycloalkyl group containing from 3 to 15 carbon atoms wherein at least one carbon atom is substituted with an atom or molecule selected from the group consisting of O or S-(O)0-2.
-
- wherein R1′, R1″, R1′″, and R1″41 may each individually be selected from the group halogen, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, and cyano, and X is O, or S (—O)0-2, alternately, R1′″and R1″″ may be a single oxygen atom. More preferably, R1′, R1″, R1′″, and R1″″ are each individually selected from the group hydrogen, lower alkyl, and substituted lower alkyl.
- Most preferably, R1 is selected from the group consisting of 3-tetrahydrofuranyl, 3-tetrahydrothiofuranyl 4-pyranyl, and 4 thiopyranyl.
- Most preferred compositions of this invention include: 2-{6-[((3 R)oxolan-3-yl)amino]-8-[(methylethyl)amino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3 ,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-enylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hy droxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-ynylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(ethylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(propylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(butylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-[benzylamino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(methylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-hydroxymethyl)oxolane-3,4-diol; and (5- {6-[((3 R)oxolan-3-yl)amino]-8-(ethylamino)purin-9-yl}(2 R,3 R,4 R,5 R)-3,4-diacetyloxyoxolan-2-yl)methyl acetate.
- The following definitions apply to terms as used herein.
- “Halo” or “Halogen”—alone or in combination means all halogens, that is, chloro (Cl), fluoro (F), bromo (Br), iodo (J).
- “Hydroxyl” refers to the group —OH.
- “Thiol” or “mercapto” refers to the group —SH.
- “Alkyl”—alone or in combination means an alkane-derived radical containing from 1 to 20, preferably 1 to 15, carbon atoms (unless specifically defined). It is a straight chain alkyl, branched alkyl or cycloalkyl. Preferably, straight or branched alkyl groups containing from 1 -15, more preferably 1 to 8, even more preferably 1-6, yet more preferably 1-4 and most preferably 1-2, carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl and the like. The term “lower alkyl” is used herein to describe the straight chain alkyl groups described immediately above. Preferably, cycloalkyl groups are monocyclic, bicyclic or tricyclic ring systems of 3-8, more preferably 3-6, ring members per ring, such as cyclopropyl, cyclopentyl, cyclohexyl, adamantyl and the like. Alkyl also includes a straight chain or branched alkyl group that contains or is interrupted by a cycloalkyl portion. The straight chain or branched alkyl group is attached at any available point to produce a stable compound. Examples of this include, but are not limited to, 4-(isopropyl)-cyclohexylethyl or 2-methyl-cyclopropylpentyl. A substituted alkyl is a straight chain alkyl, branched alkyl, or cycloalkyl group defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, or the like.
- “Alkenyl”—alone or in combination means a straight, branched, or cyclic hydrocarbon containing 2-20, preferably 2-17, more preferably 2-10, even more preferably 2-8, most preferably 2-4, carbon atoms and at least one, preferably 1-3, more preferably 1-2, most preferably one, carbon to carbon double bond. In the case of a cycloalkyl group, conjugation of more than one carbon to carbon double bond is not such as to confer aromaticity to the ring. Carbon to carbon double bonds may be either contained within a cycloalkyl portion, with the exception of cyclopropyl, or within a straight chain or branched portion. Examples of alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, cyclohexenyl, cyclohexenylalkyl and the like. A substituted alkenyl is the straight chain alkenyl, branched alkenyl or cycloalkenyl group defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, carboxy, alkoxycarbonyl, aryloxycarbonyl, heteroaryloxycarbonyl, or the like attached at any available point to produce a stable compound.
- “Alkynyl”—alone or in combination means a straight or branched hydrocarbon containing 2-20, preferably 2-17, more preferably 2-10, even more preferably 2-8, most preferably 2-4, carbon atoms containing at least one, preferably one, carbon to carbon triple bond. Examples of alkynyl groups include ethynyl, propynyl, butynyl and the like. A substituted alkynyl refers to the straight chain alkynyl or branched alkenyl defined previously, independently substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di- substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di- substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, or the like attached at any available point to produce a stable compound.
- “Alkyl alkenyl” refers to a group —R—CR′=CR′″R″″. where R is lower alkyl, or substituted lower alkyl, R′, R′″, R″″ may independently be hydrogen, halogen, lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined below.
- “Alkyl alkynyl” refers to a groups —RC≡CR′ where R is lower alkyl or substituted lower alkyl, R′ is hydrogen, lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined below.
- “Alkoxy” denotes the group —OR, where R is lower alkyl, substituted lower alkyl, acyl, aryl, substituted aryl, aralkyl, substituted aralkyl, heteroalkyl, heteroarylalkyl, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, or substituted cycloheteroalkyl as defined.
- “Alkylthio” denotes the group —SR, —S(O)n=1−2−R, where R is lower alkyl, substituted lower alkyl, aryl, substituted aryl, aralkyl or substituted aralkyl as defined herein.
- “Acyl” denotes groups —C(O)R, where R is hydrogen, lower alkyl substituted lower alkyl, aryl, substituted aryl and the like as defined herein.
- “Aryloxy” denotes groups —OAr, where Ar is an aryl, substituted aryl, heteroaryl, or substituted heteroaryl group as defined herein.
- “Amino” denotes the group NRR′, where R and R′ may independently by hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, or substituted hetaryl as defined herein or acyl.
- “Amido” denotes the group —C(O)NRR′, where R and R′ may independently by hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, substituted hetaryl as defined herein.
- “Carboxyl” denotes the group —C(O)OR, where R is hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, hetaryl, and substituted hetaryl as defined herein.
- “Aryl”—alone or in combination means phenyl or naphthyl optionally carbocyclic fused with a cycloalkyl of preferably 5-7, more preferably 5-6, ring members and/or optionally substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, or the like.
- “Substituted aryl” refers to aryl optionally substituted with one or more functional groups, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Heterocycle” refers to a saturated, unsaturated, or aromatic carbocyclic group having a single ring (e.g., morpholino, pyridyl or furyl) or multiple condensed rings (e.g., naphthpyridyl, quinoxalyl, quinolinyl, indolizinyl or benzo[b]thienyl) and having at least one hetero atom, such as N, O or S, within the ring, which can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Heteroaryl”—alone or in combination means a monocyclic aromatic ring structure containing 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing one or more, preferably 1-4, more preferably 1-3, even more preferably 1-2, heteroatoms independently selected from the group O, S, and N, and optionally substituted with 1 to 3 groups or substituents of halo, hydroxy, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, acyloxy, aryloxy, heteroaryloxy, amino optionally mono- or di-substituted with alkyl, aryl or heteroaryl groups, amidino, urea optionally substituted with alkyl, aryl, heteroaryl or heterocyclyl groups, aminosulfonyl optionally N-mono- or N,N-di-substituted with alkyl, aryl or heteroaryl groups, alkylsulfonylamino, arylsulfonylamino, heteroarylsulfonylamino, alkylcarbonylamino, arylcarbonylamino, heteroarylcarbonylamino, or the like. Heteroaryl is also intended to include oxidized S or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen. A carbon or nitrogen atom is the point of attachment of the heteroaryl ring structure such that a stable aromatic ring is retained. Examples of heteroaryl groups are pyridinyl, pyridazinyl, pyrazinyl, quinazolinyl, purinyl, indolyl, quinolinyl, pyrimidinyl, pyrrolyl, oxazolyl, thiazolyl, thienyl, isoxazolyl, oxathiadiazolyl, isothiazolyl, tetrazolyl, imidazolyl, triazinyl, faranyl, benzofuryl, indolyl and the like. A substituted heteroaryl contains a substituent attached at an available carbon or nitrogen to produce a stable compound.
- “Heterocyclyl”—alone or in combination means a non-aromatic cycloalkyl group having from 5 to 10 atoms in which from 1 to 3 carbon atoms in the ring are replaced by heteroatoms of O, S or N, and are optionally benzo fused or fused heteroaryl of 5-6 ring members and/or are optionally substituted as in the case of cycloalkyl. Heterocycyl is also intended to include oxidized S or N, such as sulfinyl, sulfonyl and N-oxide of a tertiary ring nitrogen. The point of attaclunent is at a carbon or nitrogen atom. Examples of heterocyclyl groups are tetrahydrofuranyl, dihydropyridinyl, piperidinyl, pyrrolidinyl, piperazinyl, dihydrobenzofuryl, dihydroindolyl, and the like. A substituted hetercyclyl contains a substituent nitrogen attached at an available carbon or nitrogen to produce a stable compound.
- “Substituted heteroaryl” refers to a heterocycle optionally mono or poly substituted with one or more functional groups, e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Aralkyl” refers to the group —R—Ar where Ar is an aryl group and R is lower alkyl or substituted lower alkyl group. Aryl groups can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Heteroalkyl” refers to the group —R—Het where Het is a heterocycle group and R is a lower alkyl group. Heteroalkyl groups can optionally be unsubstituted or substituted with e.g., halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Heteroarylalkyl” refers to the group —R—HetAr where HetAr is an heteroaryl group and R lower alkyl or substituted lower alkyl. Heteroarylalkyl groups can optionally be unsubstituted or substituted with, e.g., halogen, lower alkyl, substituted lower alkyl, alkoxy, alkylthio, acetylene, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Cycloalkyl” refers to a divalent cyclic or polycyclic alkyl group containing 3 to 15 carbon atoms.
- “Substituted cycloalkyl” refers to a cycloalkyl group comprising one or more substituents with, e.g., halogen, lower alkyl, substituted lower alkyl, alkoxy, alkylthio, acetylene, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Cycloheteroalkyl” refers to a cycloalkyl group wherein one or more of the ring carbon atoms is replaced with a heteroatom (e.g., N, O, S or P).
- Substituted cycloheteroalkyl” refers to a cycloheteroalkyl group as herein defined which contains one or more substituents, such as halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Alkyl cycloalkyl” denotes the group —R-cycloalkyl where cycloalkyl is a cycloalkyl group and R is a lower alkyl or substituted lower alkyl. Cycloalkyl groups can optionally be unsubstituted or substituted with e.g. halogen, lower alkyl, lower alkoxy, alkylthio, acetylene, amino, amido, carboxyl, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
- “Alkyl cycloheteroalkyl” denotes the group —R-cycloheteroalkyl where R is a lower alkyl or substituted lower alkyl. Cycloheteroalkyl groups can optionally be unsubstituted or substituted with e.g. halogen, lower alkyl, lower alkoxy, alkylthio, amino, amido, carboxyl, acetylene, hydroxyl, aryl, aryloxy, heterocycle, substituted heterocycle, hetaryl, substituted hetaryl, nitro, cyano, thiol, sulfamido and the like.
-
-
-
-
- Compound6 can be obtained by the direct acetylation of compound 5 (Scheme 4).
- This invention also includes pro-drugs of the A1 agonist compositions of this invention. A pro-drug is a drug which has been chemically modified and may be biologically inactive at its site of action, but which will be degraded or modified by one or more enzymatic or in vivo processes to the bioactive form. The pro-drugs of this invention should have a different pharmacokinetic profile to the parent enabling improved absorption across the mucosal epithelium, better salt formulation and/or solubility and improved systemic stability. The compounds of this invention may be preferably modified at one or more of the hydroxyl groups to form pro-drugs. The modifications may be (1) ester or carbamate derivatives which may be cleaved by esterases or lipases, for example; (2) peptides which may be recognized by specific or non specific proteinase; or (3) derivatives that accumulate at a site of action through membrane selection or a pro-drug form or modified pro-drug form, or any combination of (1) to (3) above.
- If a compound of this invention contains a basic group, then corresponding acid addition salt may be prepared. Acid addition salts of the compounds are prepared in a standard manner in a suitable solvent from the parent compound and an excess of acid, such as hydrochloric, hydrobromic, sulfuric, phosphoric, acetic, maleic, succinic, or methanesulfonic. The hydrochloric salt form is especially useful. If a compound of this invention contains an acidic group, then corresponding cationic salts may be prepared. Typically the parent compound is treated with an excess of an alkaline reagent, such as hydroxide, carbonate or alkoxide, containing the appropriate cation. Cations such as Na+, K+, Ca+2 and NH4 + are examples of cations present in pharmaceutically acceptable salts. Certain of the compounds form inner salts or zwitterions which may also be acceptable.
- The compositions of this invention are useful for treating a variety of mammalian disorders and preferably human disorders that are mediated by an A1 adenosine receptor. For example, the compositions of this invention are useful for modifying cardiac activity in mammals experiencing a coronary electrical disorder that can be treated by stimulating an A1 adenosine receptor. Examples of coronary electrical disorders that can be treated by the compositions of this invention include supraventricular tachycardias, atrial fibrillation, atrial flutter, and AV nodal re-entrant tachycardia. Furthermore, orally active A1 agonists of this invention that demonstrate an excellent safety profile in treating supraventricular arrhythmias may also be used as a prophylactic for those at high risk of a myocardial ischemia.
- The compositions of this invention are also useful for modifying adipocyte function by stimulating an A1 adenosine receptor that leads to diminished release of NEFA and increased release of leptin. Disease states related to adipocyte function that can be modified using compositions of this invention include diabetes, and obesity.
- In skeletal muscle cells, A1 AdoR agonists mediate a synergistic stimulation of glucose uptake and transport by insulin (Vergauwen, L. et al, J Clin. Invest. 1994, 93, 974-81; Challiss, R. A. et al, Eur. J. Pharacol., 1992, 226, 121-8). Another therapeutic utility of compositions of this invention is more efficient regulation of glucose and a decrease of circulating levels of insulin in patients afflicted with diabetes.
- The A1 receptor agonist, R-PIA, has been shown to increase the leptin released from white adipocytes and augment insulin-stimulated leptin production (M. Ozeck Master's Thesis Univ. of Florida 1999 with L. Belardinelli). Evidence suggests that catecholamines inhibit the production of leptin from adipocytes through activation of P-adrenergic receptors. The anti-β-adrenergic effects of A1 agonists on the adipocytes are believed to play a role in the increased release of leptin. The functional role of leptin is multifaceted including decreased appetite, stimulated energy utilization, and increased fertility.
- The compositions of this invention may also be used to provide central nervous system neuroprotection by stimulating an A1 adenosine receptor. Central nervous system disorders that may be treated using the compositions of this invention include epilepsy, and stroke.
- In the kidney, there is evidence that stimulation of the A1 AdoR promotes sodium retention, promotes exchange of sodium in urine for potassium, and reduces glomerular filtration rate as sodium excretion increases (Gellai, M. et al, JPET, 1998, 286, 1191-6; Wilcox, C. S. et al, J. Am. Soc. Nephrol., 1999, 10, 714-720). It is believed that these responses are elicited by chronic local production of adenosine. That is, in the kidney there is a tonic effect of adenosine to stimulate the A1 AdoR. Another clinical utility of compositions of this invention, therefore, is the selective antagonism of the A1 AdoR in the kidney to inhibit sodium retention, inhibit the exchange of sodium for potassium, and preserve kidney glomerular filtration rate when sodium excretion rises to yield a potassium sparring diuretic that preserves renal function.
- The compositions of this invention are further useful for providing cardiomyocyte protection from ischemic events by stimulating an A1 adenosine receptor. Ischemic events treatable using the compositions of this invention include stable angina, unstable angina, cardiac transplant, and myocardial infarction.
- An important aspect of compounds of this invention is that each compound has an intrinsic efficacy associated with it (for a discussion see T. P. Kenakin Stimulus Response Mechanisms. In Pharmacological Analysis of Drug-Receptor Interaction, Ed. Kenakin, T. P. New York: Raven Press, p 39-68). This intrinsic efficacy is not defined by it's affinity for the receptor, but it is defined as the quantitative effect of the compound to activate a given effector system (eg. cAMP production) in a given cell type. The intrinsic efficacy of a given compound may vary from cell type to cell type and/or from effector system to effector system. When a compound has an intrinsic efficacy lower than a full agonist (i.e. submaximal) than the agonist is called a partial agonist. Thus, a partial agonist is a molecule that binds to a receptor and elicits a response that is smaller than that of a full agonist (submaximal), but also competitively antagonizes the response(s) elicited by a full agonist. The tonic action of adenosine with resepct to kidney function is a prime example where a partial A1 agonist be expected to act as antagonists (e.g. adenosine). The tonic action of adenosine with respect to kidney function is a prime example where a partial A1 agonist could be expected to act as an antagonist. The compounds of this invention are believed to have therapeutically useful affinities for the adenosine A1 receptor, and they will have a range of intrinsic efficacies from full agonist to partial agonist. That is, some compounds may have no effect with respect to a given effector system in a given cell type, but be a full agonist in another cell type and/or effector system. The reason for such variable pharmacological behavior relates to the magnitude of the receptor reserve for the A1 adenosine receptor in any given cell type (eg. AV nodal cells vs. adipocytes) and for a given response. The receptor reserve (spare receptor capacity) is the total number of receptors minus the fraction of receptors that is required to induce the maximal response using a full agonist (L. E. Limbird, Cell Surface Receptors: A Short Course on Theory and Methods, Kluwer Acad. Pub. 1996, Boston, Mass.). Therefore, the agonist could be a full agonist at eliciting a response, and a partial agonist for eliciting another response in other tissue or cells and still be an antagonist or lack activity for a third response in another tissue or cell. Consequently, a partial agonist targeted to a selected target is likely to cause fewer side effects than a full agonist. As a corollary, a full agonist elicits all the effects mediated by the respective receptor, whereas this is not necessarily the case of a partial agonist. The compounds of this invention based on their affinity for the A1 receptor and their potency and selectivity to elicit A1 receptor mediated responses have the potential for therapeutic intervention in the multiple disease states described above.
- Partial A1 agonists may have an added benefit for chronic therapy because they will be less likely to induce desensitization of the A1 receptor (R. B. Clark, B. J. Knoll, R. Barber TiPS, Vol. 20 (1999) p. 279-286) and to cause side effects. Chronic administration of a full agonist (R-N6-phenylisopropyladenosine, R-PIA) for 7 days led to a desensitization of the A1 receptor in terms of the dromotropic response in guinea pigs (note: a decrease in receptor number was observed - D. M. Dennis, J. C. Shryock, L. Belardinelli JPET, Vol. 272 (1995) p. 1024-1035). The A1 agonist induced inhibitory effect on the production of cAMP by adenylate cyclase in adipocytes has been shown to desensitize upon chronic treatment with an A1 agonist as well (W. J. Parsons and G. L. Stiles J. Biol. Chem. Vol. 262 (1987) p. 841-847).
- The compositions of this invention may be administered orally, intravenously, through the epidermis, bolus, nasally, by inhalation or by any other means known in the art for administering a therapeutic agents. The method of treatment comprises the administration of an effective quantity of the chosen compound, preferably dispersed in a pharmaceutical carrier. Dosage units of the active ingredient are generally selected from the range of 0.01 to 100 mg/kg, but will be readily determined by one skilled in the art depending upon the route of administration, age and condition of the patient.
- Pharmaceutical compositions including the compounds of this invention, and/or derivatives thereof, may be formulated as solutions or lyophilized powders for parenteral administration. Powders may be reconstituted by addition of a suitable diluent or other pharmaceutically acceptable carrier prior to use. If used in liquid form the compositions of this invention are preferably incorporated into a buffered, isotonic, aqueous solution. Examples of suitable diluents are normal isotonic saline solution, standard 5% dextrose in water and buffered sodium or ammonium acetate solution. Such liquid formulations are suitable for parenteral administration, but may also be used for oral administration. It may be desirable to add excipients such as polyvinylpyrrolidinone, gelatin, hydroxycellulose, acacia, polyethylene glycol, mannitol, sodium chloride, sodium citrate or any other excipient known to one of skill in the art to pharmaceutical compositions including compounds of this invention. Alternatively, the pharmaceutical compounds may be encapsulated, tableted or prepared in an emulsion or syrup for oral administration. Pharmaceutically acceptable solid or liquid carriers may be added to enhance or stabilize the composition, or to facilitate preparation of the composition. Liquid carriers include syrup, peanut oil, olive oil, glycerin, saline, alcohols and water. Solid carriers include starch, lactose, calcium sulfate, dihydrate, teffa alba, magnesium stearate or stearic acid, talc, pectin, acacia, agar or gelatin. The carrier may also include a sustained release material such as glycerol monostearate or glycerol distearate, alone or with a wax. The amount of solid carrier varies but, preferably, will be between about 20 mg to about 1 gram per dosage unit. The pharmaceutical dosages are made using conventional techniques such as milling, mixing, granulation, and compressing, when necessary, for tablet forms; or milling, mixing and filling for hard gelatin capsule forms. When a liquid carrier is used, the preparation will be in the form of a syrup, elixir, emulsion or an aqueous or non-aqueous suspension. Such a liquid formulation may be administered directly or filled into a soft gelatin capsule.
- The Examples which follow serve to illustrate this invention. The Examples are intended to in no way limit the scope of this invention, but are provided to show how to make and use the compounds of this invention.
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-(ethylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (Compound 5)
- 5 - {6-[((3 R)oxolan-3 -yl)amino]purin-9-yl}(4 S ,2 R,3 R,5 R)-4-acetoxy-2-(acetoxymethyl)oxolane-3yl-acetate: 2-{6-[((3 R)oxolan-3-yl)amino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl) oxolane-3,4-diol (2) was prepared from 6-Chloro purine riboside as described in U.S. Pat. No. 5,789,416, the specification of which is incorporated herein by reference. To a solution of compound 2 (1.68 g, 5 mmol) and dimethylaminopyridine (100 mg, 0.82 mmol) in pyridine (10 mL) at 23° C. was added acetic anhydride (1 mL, 10.6 mmol). After 3 h at 23° C., the reaction was concentrated in vacuo . The residue was dissolved in methylene chloride (100 mL), washed with water (3×20 mL), and dried (Na2SO4). After concentration in vacuo, the residue was purified by flash chromatography (methylene chloride: methanol 20:1 followed by 9:1) to afford compound 3.
- Synthesis of 5- {6-[((3 R)oxolan-3-yl)amino]-8-chloropurin-9-yl}(4 S,2 R,3 R,5 R)-4-acetoxy-2-(acetoxymethyl)oxolane-3yl-acetate: To a stirred solution of compound 3 (1 g, 2.16 mmol) in 1,2-dichloroethane (10 mL) was added N-chlorosuccinimide (1 g, 7.5 mmol) and the reaction was warmed to 55° C. for 24 h. The solvent was evaporated and the product purified by flash chromatography (methylene chloride: methanol 100:0 followed by 95:5) to afford compound4.
- To a stirred solution of compound4 (100 mg, 0.2 mmol) in dioxane (0.5 mL) ethylamine (75% aqueous solution, 3 mL) was added and the reaction was warmed to 65° C. for 16 h. The resulting mixture was evaporated to dryness and the product purified by preparative TLC using methylene chloride:methanol (95:5) as solvent to afford compound 5: 1H NMR (CD3OD)δ1.25(τ, 3 H), 1.80-1.90(m, 1 H), 2.30-2.40 (m, 1 H), 3.40 (q, 2 H), 3.50-3.90 (m, 4 H), 3.90-4.00 (m,2 H), 4.10-4.15 (m, 1 H), 4.20-4.25 (m, 1 H), 4.65-4.80 (m, 2 H), 5.95 (d, 1 H), 7.95 (s, 1 H).
- [MS: 381.25 (M+1)].
-
- (5-{6- [((3 R)oxolan-3-yl)amino]-8-(ethylamino)purin-9-yl}(2 R,3 R,4 R,5 R)-3,4-diacet yloxyoxolan-2-yl)methyl acetate (compound6)
- Compound6 was prepared (Scheme 4) as described for the synthesis of compound 3 in Example 1, above.
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-(methylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyoxolanae-3,4adioe (compound 7)
-
- 2-{6- [((3 R)oxolan-3-yl)amino]-8-(propylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound8)
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-(butylamino)purin-9-yl}(4S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound9)
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-[benzylamino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound10)
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-[(methylethyl)amino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound11)
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-enylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound12)
- Compound12 was prepared as described in example 1 substituting allylamine for ethylamine [(MS: 393.7 (M+1)].
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-ynylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound13)
-
- 2-{6-[((3 R)oxolan-3-yl)amino]-8-methoxypurin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol (compound13)
- To a solution of compound4 in 1 mL of dry methanol was added 3 mL of 0.5 M solution of sodium methoxide in methanol. The reaction mixture was refluxed for 30 min. TLC (5% MeOH: 95% DCM) showed that the reaction was completed. The reaction mixture was cooled and quenched with a few drops of glacial acetic acid and the solvent was evaporated. The residue was taken up in methanol and analyzed by mass spectrometer [MS 368.2 (M+1) and 390.2 (M+23)].
- Binding Assays - DDT1 Cells
- Cell Culture
- DDT cells (hamster vas deferens smooth muscle cell line) were grown as monolayers in petri dishes using Dulbecco's Modified Eagle's Medium (DMEM) containing 2.5 μg ml−1 amphotericin B, 100 U ml−1 penicillin G, 0.1 mg ml−1 streptomycin sulfate and 5% fetal bovine serum in a humidified atmosphere of 95% air and 5% CO2. Cells were subcultured twice weekly by dispersion in Hank's Balanced Salt Solution (HBSS) without the divalent cations and containing 1 mM EDTA. The cells were then seeded in growth medium at a density of 1.2×15 cells per plate and experiments were performed 4 days later at approximately one day preconfluence.
- Membrane Preparations
- Attached cells were washed twice with HBSS (2×10 ml), scraped free of the plate with the aid of a rubber policeman in 5 ml of 50 mM Tris-HCl buffer pH 7.4 at 4° C. and the suspension homogenized for 10 s. The suspension was then centrifuged at 27,000 x g for 10 min. The pellet was resuspended in homogenization buffer by vortexing and centrifuged as described above. The final pellet was resuspended in 1 vol of 50 mM Tris-HCl buffer pH 7.4 containing 5 mM MgCl2 for A1 AdoR assays. For the [35S]GTPγS binding assay the final pellet was resuspended in 50 mM Tris-HCl pH 7.4 containing 5 mM MgCl2, 100 mM NaCl and 1 mM dithiothreitol. This membrane suspension was then placed in liquid nitrogen for 10 min, thawed and used for assays. The protein content was determined with a Bradford™ Assay Kit using bovine serum albumin as standard.
- Competitive Binding Assay
- Pig striatum were prepared by homogenation in 50 mM Tris buffer ( 5x volume of tissue mass pH=7.4). After centrifugation at 19,000 rpm for 25 minutes at 4° C., the supernatant was discarded, and the process was repeated twice. Compositions of this invention were assayed to determine their affinity for the A1 receptor in a pig striatum membrane prep or a DDT1 membrane prep. Briefly, 0.2 mg of pig striatal membranes or DDT1 cell membranes were treated with adenosine deaminase and 50 mM Tris buffer (pH=7.4) followed by mixing. To the pig membranes was added 2 μL of serially diluted DMSO stock solution of the compounds of this invention at concentrations ranging from 100 microM to 10 nM. The control received 2 microL of DMSO alone, then the antagonist [3H] 8-cyclopentylxanthine (CPX) for pig striatum or the agonist [3H] 2-chloro-6-cyclopentyladenosine (CCPA) for DDT1 membranes in Tris buffer (50 mM, pH of 7.4) was added to achieve a final concentration of 2 nM. After bation at 23 C. for 2 h, then the solutions were filtered using a membrane harvester using iple washing of the membranes (3 x). The filter disks were counted in scintillation cocktail rding the amount of displacement of tritiated CPX or by the competitive binding ositions of this invention. Greater than a 5 point curve was used to generate Ki's and the er of experiments is indicated in the column marked in Table 1, below:
TABLE 1 Compound # Ki-DDT1 cell membrane Ki-Pig Striatum 5 171 mM 137 nM 7 — 799 nM 8 — 1040 nM 9 — 2840 nM 10 — 7470 nM - [35S]GTPYS Binding Assays
- A1-agonist stimulated [35S] GTPγS binding was determined by a modification of the method described by Giersckik et al. (1991) and Lorenzen et al. (1993). Membrane protein (30-50 μg) was incubated in a volume of 0.1 ml containing 50 mM Tris-HCl buffer pH 7.4, 5 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol, 0.2 units ml−1 adenosine deaminase, 0.5% BSA, 1 mM EDTA, 10 mM GDP, 0.3 nM [35S]GTPγS and with or without varying concentrations of CPA for 90 min at 30° C. Nonspecific binding was determined by the addition of 10 μM GTPγS. Agonist stimulated binding was determined as the difference between total binding in the presence of CPA and basal binding determined in the absence of CPA. Previous reports have shown that agonist stimulated [35S]GTPγS binding was dependent on the presence of GDP (Gierschik et al., 1991; Lorenzen et al., 1993; Traynor & Nahorski, 1995). In preliminary experiments, it was found that 10 μM GDP gave the optimal stimulation of CPA dependent [35S]GTPγS binding and this concentration was therefore used in all studies. In saturation experiments, 0.5 nM [35S]GTPγS was incubated with 0.5-1000 nM GTPγS. At the end of the incubation, each suspension was filtered and the retained radioactivity determined as described above. Results are presented normalized to the full agonist N-6-cyclopentyladenosine, CPA.
TABLE 2 Compound # GIPγS CPA 100% 5 89% 11 68% 12 77% 13 95% - cAMP Assay
- A scintillation proximity assay (SPA) using rabbit antibodies directed at cAMP using an added tracer of adenosine 3′,5′-cyclic phosphoric acid 2′-O-succinyl-3-[125I] iodotyrosine methyl ester and fluoromicrospheres containing anti-rabbit specific antibodies as described by Amersham Pharmacia Biotech (Biotrak cellular communication assays). Briefly, DDT1 cells were cultured in clear bottomed 96 well microtiter plates with opaque wells at concentrations between 104 to 106 cells per well in 40 μl of HBSS at 37° C. (5% CO2 and 95% humidity). The partial or full A1 agonists (5 μl )of this invention were incubated at various concentrations with the DDT1 cells in the presence of rolipram (50 μM), and 5 μM forskolin for 10 min at 37° C. The cells were immediately lysed by treatment 5 μl of 10% dodecyltrimethylammonium bromide followed by shaking using microplate shaker. After incubation of the plate for 5 minutes, an immunoreagent solution (150 μl containing equal volumes of tracer, antiserum, and SPA fluorospheres) was added to each well followed by sealing the plate. After 15-20 h at 23° C., the amount of bound [125I] cAMP to the fluoromicrospheres was determined by counting in a microtitre plate scintillation counter for 2 minutes. Comparison of counts with standard curves generated for cAMP using a similar protocol afforded the cAMP present after cell lysis. Results are presented normalized to the full agonist N-6-cyclopentyladenosine, CPA. Thus, the full agonist CPA diminished the amount of forskolin induced cAMP generation back to basal levels.
TABLE 3 Compound # cAMP CPA 100% 11 37% 12 42% 13 41%
Claims (35)
1. A composition of matter having the formula:
wherein X1=O, S, NR7;
R1 is a monocyclic or polycyclic heterocyclic group containing from 3 to 15 carbon atoms wherein at least one carbon atom is substituted with an atom or a molecule selected from the group consisting of N, O, P and S-(O)0-2 and wherein R1 does not contain an epoxide group;
R2 is selected from the group consisting of hydrogen, halo, CF3, and cyano;
R3, R4, and R5 are each individually selected from the group consisting of hydrogen, —(CO)—R′, —(CO)—R″, and —(CO)—R′″ wherein R′, R″, and R′″ are each individually selected from the group consisting of C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, which alkyl, alkenyl, alkynyl, aryl, heterocyclyl, and heteroaryl are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, NO2, alkyl, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, SR20, N(R20)2, S(O)R22, SO2R22, SO2N(R20)2, SO2NR20COR22, SO2NR20CO2R22, SO2NR20CON(R20)2, N(R20)2, NR20COR22, NR20CO2R22, NR20CON(R20)2, NR20C(NR20)NHR23, COR20, CO2R20, CON(R20)2, CONR20SO2R22, NR20SO2R22, SO2NR20CO2R22, OCONR20SO2R22, OC(O)R20, C(O)OCH2OC(O)R20, and OCON(R20)2 and each optional heteroaryl, aryl, alkyl, and heterocyclyl substituent is optionally further substituted with halo, NO2, alkyl, CF3, amino, mono- or di- alkylamino, alkyl amide, aryl amide, heteroaryl amide, NR20COR22, NR20SO2R22, COR20, CO2R20, CON(R20)2, NR20CON(R20)2, OC(O)R20, OC(O)N(R20)2, SR20, S(O)R22, SO2R22, SO2N(R20)2, CN, or OR20;
R6 and R7 are each individually selected from the group consisting of hydrogen, C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, heterocyclyl and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, NO2, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, SR20, S(O)R22, SO2R22, SO2N(R20)2, SO2NR20COR22, SO2NR20CO2R22, S(O)3R2, P(O)(OR20)2, SO2NR20CON(R20)2, NR20COR22, NR20CO2R22, NR20CON(R20)2, NR20C(NR20)NHR23, COR20, CO2R20, CON(R20)2, CONR20SO2R22, NR20SO2R22, SO2NR20CO2R22, OCONR20SO2R22, OC(O)R20, C(O)OCH2OC(O)R20 , and OCON(R20)2 and wherein each optional heteroaryl, aryl, and heterocyclyl substituent is optionally substituted with halo, NO2, alkyl, CF3, amino, mono- or di- alkylamino, alkyl amide, aryl amide, heteroaryl amide, NCOR22, NR20SO2R22, COR20, CO2R20, CON(R20)2, NR20CON(R20)2, OC(O)R20, OC(O)N(R20)2, SR20, S(O)3R20, P(O)(OR20)2, S(O)R22, SO2R22, SO2N(R20)2, CN, or OR20;
R20 is selected from the group consisting of H, C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or aryl or heteroaryl amide, CN, O-C1-6 alkyl, CF3, aryl, and heteroaryl; and
R22 is selected from the group consisting of C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, heterocyclyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl amide, aryl amide, heteroaryl amide, CN, O-C1-6 alkyl, CF3, aryl, and heteroaryl.
2. The composition of claim 1 wherein R3, R4, and R5 are each individually selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, and —(CO)-R′″ wherein R′, R″, and R′″ are each individually selected from the group consisting of C1-15 alkyl, heterocyclyl, aryl, and heteroaryl, which alkyl, aryl, heterocyclyl, and heteroaryl are optionally substituted with from 1 to 2 substituents independently selected from the group of halo, N02, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, N(R20)2, S(O)R22, SO2R22, SO2N(R20)2, NR20COR22, NR20CO2R22, NR20CON(R20)2, NR20C(NR20)NHR23, COR20, CO2R20, CON(R20)2, CONR20SO2R22, NR20SO2R22 and wherein each optional heteroaryl, aryl, and heterocyclyl substituent is further optionally substituted with halo, NO2, alkyl, CF3, amino, mono- or di- alkylamino, CN, or OR20;
R6 is selected from the group consisting of hydrogen, and C1-15 alkyl optionally substituted with aryl;
R7 is selected from the group consisting of hydrogen, C1-15 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, heterocyclyl and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, heterocyclyl, aryl, heteroaryl, CF3, CN, OR20, SR20, N(R20)2, S(O)R22, S(O)3R20, P(O)(OR20)2, SO2R22, SO2N(R20)2, NR20CO2R22, NR20CON(R20)2, COR20, CO2R20, CON(R20)2, and wherein the optional heteroaryl, aryl, and heterocyclyl substituents are further optionally substituted with halo, alkyl, CF3, CO2R20, S(O)3R20, P(O)(OR20)2, amino, mono- or di- alkylamino, CN, or OR20;
R20 is selected from the group consisting of H, C1-15 alkyl, aryl, and heteroaryl, which alkyl, aryl, and heteroaryl are optionally substituted with from 1 to 2 substituents independently selected from the group consisting of halo, alkyl, mono- or dialkylamino, CN, O-C1-6 alkyl, CF3; and
R22 is selected from the group consisting of C1-15 alkyl, aryl, and heteroaryl, which alkyl, aryl, and heteroaryl are optionally substituted with from 1 to 2 substituents independently selected from halo, alkyl, mono- or dialkylamino, alkyl or CN, O-C1-6 alkyl, and CF3.
3. The composition of claim 1 wherein X1=NR7;
R2 is hydrogen;
R3, R4, and R5 are each individually selected from the group consisting of hydrogen, —(CO)-R′, —CO)-R″, and —(CO)-R′″ wherein R′, R″, and R′″ are independently selected from the group consisting of C1-10 alkyl, aryl, and heteroaryl, which alkyl, aryl, and heteroaryl are optionally substituted with from 1 to 2 substituents independently selected from the group of halo, NO2, aryl, heteroaryl, CF3, CN, OR20, N(R20)2, S(O)R22, SO2R22, NR20COR22, COR20, CO2R20, CON(R20)2, NR20SO2R22, and wherein each optional heteroaryl, aryl, and heterocyclyl substituent is further optionally substituted with halo, NO2, alkyl, and CF3;
R6 is selected from the group consisting of hydrogen, and C1-3 alkyl that is optionally substituted with aryl;
R7 is selected from the group consisting of hydrogen, C1-8 alkyl, C2-15 alkenyl, C2-15 alkynyl, heterocyclyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, heterocyclyl and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, aryl, heteroaryl, CF3, CN, OR20, SR20, CO2R20, S(O)3R20, SO2N(R20)2, NR20CO2R22, NR20CON(R20)2, COR20, and wherein the optional heteroaryl, and aryl substituents are each further optionally substituted with halo, alkyl, CF3, S(O)3R20, CO2R20, amino, mono- or di- alkylamino, CN, and OR20;
R20 is selected from the group consisting of H, C1-6 alkyl, and aryl, which alkyl, and aryl, are optionally substituted with 1 substituent selected from halo, alkyl, mono- or dialkylamino, CN, O-C1-6 alkyl, and CF3; and
R22 is selected from the group consisting of C1-6 alkyl and aryl, which alkyl and aryl are optionally substituted with 1 substituent selected from halo, alkyl, mono- or dialkylamino, alkyl or CN, O-C1-6 alkyl, and CF3.
4. The composition of claim 1 wherein X1=NR7;
R2 is hydrogen;
R3, R4, and R5 are each individually selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, and —(CO)-R′″ wherein R′, R″, and R′″ are each independently selected from the group consisting of C1-6 alkyl, and aryl, which alkyl and aryl are optionally substituted with from 1 to 2 substituents each independently selected from the group of halo, NO2, aryl, CF3, CN, OR20, N(R20)2, S(O)R22, SO2R22, N(R20)2, and each optional aryl substituent is further optionally substituted with halo, NO2, alkyl, and CF3;
R6 is selected from the group consisting of hydrogen, C1-3 alkyl optionally substituted with aryl;
R7 is selected from the group consisting of hydrogen, C1-6 alkyl, C2-15 alkenyl, C2-15 alkynyl, aryl, and heteroaryl, wherein the alkyl, alkenyl, alkynyl, aryl, and heteroaryl substituents are optionally substituted with from 1 to 3 substituents independently selected from the group consisting of halo, alkyl, aryl, CF3, CN, OR20, SR20, S(O)3R20, CO2R20, NR20CO2R22, NR20CON(R20)2, and wherein each optional aryl substituent is further optionally substituted with halo, alkyl, CF3, S(O)3R20, CO2R20, amino, mono- or di- alkylamino, CN, and OR20;
R20 is selected from the group consisting of H, C1-6 alkyl, and aryl, which alkyl and aryl are optionally substituted with 1 substituent selected from halo, alkyl, mono- or dialkylamino, CN, O-C1-6 alkyl, and CF3; and
R22 is selected from the group consisting of C1-6 alkyl and aryl, which alkyl and aryl are optionally substituted with 1 substituent independently selected from halo, alkyl or CN, O-C1-6 alkyl, and CF3.
5. The composition of claim 1 wherein X1=NR7;
R2 is hydrogen;
R3 , R4, and R5 are each individually selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, and —(CO)-R′″ wherein R′, R″, and R′″ are each independently selected from the group consisting of C1-6 alkyl which alkyl is optionally substituted with 1 substituent selected from the group consisting of aryl, CF3, CN, OR20, N(R20)2, and each optional aryl substituent is further optionally substituted with halo, NO2, alkyl, CF3;
R6 is selected from the group consisting of hydrogen, and C1-3 alkyl;
R7 is selected from the group consisting of hydrogen, C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with from 1 to 2 substituents independently selected from the group consisting of alkyl, aryl, CF3, OR20, SR20, CO2R20, S(O)3R20, and wherein each optional aryl substituent is further optionally substituted with halo, alkyl, CF3, S(O)3R20, CO2R20, amino, mono- or di- alkylamino, CN, or OR20; and
R20 is selected from the group consisting of H, and C1-6 alkyl.
6. The composition of claim 1 wherein X1=NR7; wherein R2 is hydrogen; wherein R3, R4, and R5 are independently selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, or —(CO)-R′″ wherein R′, R″, and R′″ are independently selected from the group consisting of C1-6 alkyl;
wherein R6 is independently selected from the group consisting of hydrogen, or C1-3 alkyl;
wherein R7 is independently selected from the group consisting of hydrogen, C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with from 1 substituent independently selected from the group consisting of alkyl, aryl, CF3, OR20, SR20, CO2R20, S(O)3R20, and wherein optional aryl substituent is optionally substituted with halo, alkyl, CF3;
R20 is a member selected from the group consisting of H, C1-6 alkyl.
7. The composition of claim 1 wherein X1=NR7; wherein R2 is hydrogen; wherein R3, R4, and R5 are independently selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, or —CO)-R′″ wherein R′, R″, and R′″=0 are independently selected from the group consisting of C1-3 alkyl;
wherein R6 is hydrogen;
wherein R7 is independently selected from the group consisting of hydrogen, , C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with from 1 substituent independently selected from the group consisting of alkyl or aryl.
8. The composition of claim 1 wherein X1=NR7;
R2 is hydrogen;
R3, R4, and R5 are each independently selected from the group consisting of hydrogen, —(CO)-R′, —(CO)-R″, and —(CO)-R′″ wherein R′, R″, and R′″ are each methyl;
R6 is hydrogen;
R7 is selected from the group consisting of hydrogen, C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl.
9. The composition of claim I wherein X1=NR7;
R2 is hydrogen;
R3, R4, and R5 and R6 are each hydrogen; and
R7 is independently selected from the group consisting of hydrogen, C1-6 alkyl, C2-4 alkenyl, and C2-4 alkynyl, wherein the alkyl, alkenyl, and alkynyl substituents are optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl.
10. The composition of claim 1 wherein X1=NR7;
R2 is hydrogen;
R3, R4, R5 and R6 are each hydrogen; and
R7 is C1-6 alkyl wherein the alkyl, is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl wherein the optional aryl substituent is further optionally substituted with halo, alkyl, CF3.
11. The composition of claim 1 wherein X1=NR7;
R2, R3, R4, R5 and R6 are each hydrogen; and
R7 is C1-4 alkyl that is optionally substituted with phenyl.
12. The composition of claim 1 wherein X1=NR7;
R2, R3, R4, R5, and R6 are each hydrogen; and
R7 is C2-4 alkenyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl and aryl.
13. The composition of claim 1 wherein=NR7;
R2, R3, R4, R5, and R6 are each hydrogen; and
R7 is C2-3 alkenyl.
14. The composition of claim 1 wherein X1=NR7;
R2, R3, R4, R5, and R6 are each hydrogen; and
R7 is C2-4 alkynyl that is optionally substituted with 1 substituent selected from the group consisting of alkyl or aryl.
15. The composition of claim 1 wherein X1=NRW;
R2, R3, R4, R5, and R6 are each hydrogen; and
R7 is C2-3 alkynyl.
16. The composition of claim 1 wherein R1 is mono or polysubstituted with one or more compounds selected from the group consisting of halogen, oxo, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, cyano and mixtures thereof.
17. The composition of matter of claim 1 wherein R1 is a monocyclic, bicyclic, or tricyclic cycloalkyl group containing from 3 to 15 atoms wherein at least one carbon atom is substituted with an atom or molecule selected from the group consisting of 0 or S-(O)0-2.
18. The composition of claim 18 wherein R1 is mono or polysubstituted with one or more compounds selected from the group consisting of halogen, oxo, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, cyano and mixtures thereof.
19. The composition of claim 1 wherein R1 is:
wherein R1′, R1″, R1′″, and R1″″ are individually selected from the group halogen, hydroxyl, lower alkyl, substituted lower alkyl, alkoxy, aryl, acyl, aryloxy, carboxyl, substituted aryl, heterocycle, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, nitro, cyano and mixtures thereof and X is 0, or S (−O)0-2.
20. The composition of claim 19 wherein R1′″ and R1″″ can be a single oxygen atom.
21. The composition of claim 19 wherein R1′, R1″, R1′″ and R1″″ are individually selected from the group H, lower alkyl, substitute lower alkyl, alkoxy, aryl, and substituted aryl.
22. The composition of claim 19 wherein R1′, R1″, R1′″, and R1″″ are individually selected from the group H, lower alkyl, and substitute lower alkyl.
23. The composition of claim 1 wherein R1 is selected from the group consisting of: wherein each may individually selected from the group consisting of H, lower alkyl, and substituted lower alkyl and wherein X is 0, or S (−O)0-2.
24. The composition of claims 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 wherein R1 is selected from the group consisting of 3-tetrahydrofuranyl, 3-tetrahydrothiofuranyl, 4-pyranyl, and 4 thiopyranyl.
25. The composition of claims 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 wherein R1 is 3-tetrahydrofuranyl.
26. The composition of claim 1 wherein the compound is selected from the group consisting of 2- {6-[((3 R)oxolan-3-yl)amino]-8-[(methylethyl)amino]purin-9-yl}(4 S,2 R,3 R,5 R) 5-(hydroxymethyl)oxolane-3,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-enylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-(prop-2-ynylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2- {6-[((3 R)oxolan-3 -yl)amino]-8-(ethylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-(propylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-hydroxymethyl)oxolane-3 ,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-(butylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2- {6-[((3 R)oxolan-3-yl)amino]-8-[benzylamino]purin-9-yl}(4 S,2 R,3 R,5 R)-5-(hydroxymethyl)oxolane-3,4-diol; 2-{6-[((3 R)oxolan-3-yl)amino]-8-(methylamino)purin-9-yl}(4 S,2 R,3 R,5 R)-5-hydroxymethyl)oxolane-3,4-diol; and (5- {6-[((3 R)oxolan-3-yl)amino]-8-(ethylamino)purin-9-yl}(2 R,3 R,4 R,5 R)-3,4-diacetyloxyoxolan-2-yl)methyl acetate.
27. A method for modifying cardiac activity in a mammal experiencing a heart electrical disorder that can be treated by stimulating an A1 adenosine receptor comprising the administration of a therapeutically effective amount of the composition of claim 1 to the mammal.
28. A method for modifying mammalian adipocyte function by stimulating an A1 adenosine receptor comprising administering a therapeutically effective amount of the composition of claim 1 to the mammal.
29. A method to restore sensitivity and efficacy of insulin in a mammal by stimulating an A1 adenosine receptor comprising the administration of a therapeutically effective amount of a composition of claim 1 to the mammal.
30. A method for providing a mammal with central nervous system neuroprotection by stimulating an A1 adenosine receptor comprising administering a therapeutically effective amount of the composition of claim 1 to the mammal.
31. A method for providing a mammal with cardiomyocyte protection from ischemia by stimulating an A1 adenosine receptor comprising administering a therapeutically effective amount of the composition of claim 1 to the mammal.
32. The method of claim 27 or 28 or 29 or 30 or 31 wherein the therapeutically effective amount ranges from about 0.01 to about 100 mg/kg weight of the mammal.
33. A pharmaceutical composition of matter comprising the composition of claim 1 and one or more pharmaceutical excipients.
34. The pharmaceutical composition of matter of claim 33 wherein the pharmaceutical composition is in the form of a solution.
35. The pharmaceutical composition of matter of claim 33 wherein the pharmaceutical composition is in the form of a tablet.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/881,281 US20020045595A1 (en) | 1999-12-03 | 2001-06-14 | N6 heterocyclic 8-modified adenosine derivatives |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/454,485 US6294522B1 (en) | 1999-12-03 | 1999-12-03 | N6 heterocyclic 8-modified adenosine derivatives |
US09/881,281 US20020045595A1 (en) | 1999-12-03 | 2001-06-14 | N6 heterocyclic 8-modified adenosine derivatives |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/454,485 Continuation US6294522B1 (en) | 1999-12-03 | 1999-12-03 | N6 heterocyclic 8-modified adenosine derivatives |
Publications (1)
Publication Number | Publication Date |
---|---|
US20020045595A1 true US20020045595A1 (en) | 2002-04-18 |
Family
ID=23804799
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/454,485 Expired - Lifetime US6294522B1 (en) | 1999-12-03 | 1999-12-03 | N6 heterocyclic 8-modified adenosine derivatives |
US09/881,281 Abandoned US20020045595A1 (en) | 1999-12-03 | 2001-06-14 | N6 heterocyclic 8-modified adenosine derivatives |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/454,485 Expired - Lifetime US6294522B1 (en) | 1999-12-03 | 1999-12-03 | N6 heterocyclic 8-modified adenosine derivatives |
Country Status (20)
Country | Link |
---|---|
US (2) | US6294522B1 (en) |
EP (1) | EP1237898B1 (en) |
JP (1) | JP4021196B2 (en) |
KR (1) | KR100484987B1 (en) |
CN (1) | CN1195772C (en) |
AR (1) | AR029198A1 (en) |
AT (1) | ATE292140T1 (en) |
AU (1) | AU771242B2 (en) |
CA (1) | CA2394861C (en) |
DE (1) | DE60019164T2 (en) |
ES (1) | ES2236044T3 (en) |
HK (1) | HK1051695B (en) |
IL (2) | IL149651A0 (en) |
MX (1) | MXPA02005318A (en) |
NO (1) | NO323138B1 (en) |
NZ (1) | NZ518736A (en) |
TR (1) | TR200201470T2 (en) |
TW (1) | TWI268923B (en) |
WO (1) | WO2001042265A2 (en) |
ZA (1) | ZA200204379B (en) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9096636B2 (en) * | 1996-06-06 | 2015-08-04 | Isis Pharmaceuticals, Inc. | Chimeric oligomeric compounds and their use in gene modulation |
US7812149B2 (en) | 1996-06-06 | 2010-10-12 | Isis Pharmaceuticals, Inc. | 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations |
US20040171032A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Non-phosphorous-linked oligomeric compounds and their use in gene modulation |
US5898031A (en) | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
US20040171028A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Phosphorous-linked oligomeric compounds and their use in gene modulation |
US20040266706A1 (en) * | 2002-11-05 | 2004-12-30 | Muthiah Manoharan | Cross-linked oligomeric compounds and their use in gene modulation |
US20050118605A9 (en) * | 1996-06-06 | 2005-06-02 | Baker Brenda F. | Oligomeric compounds having modified bases for binding to adenine and guanine and their use in gene modulation |
US20040161777A1 (en) * | 1996-06-06 | 2004-08-19 | Baker Brenda F. | Modified oligonucleotides for use in RNA interference |
US20040161844A1 (en) * | 1996-06-06 | 2004-08-19 | Baker Brenda F. | Sugar and backbone-surrogate-containing oligomeric compounds and compositions for use in gene modulation |
US20040171030A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Oligomeric compounds having modified bases for binding to cytosine and uracil or thymine and their use in gene modulation |
US6214807B1 (en) * | 1999-06-22 | 2001-04-10 | Cv Therapeutics, Inc. | C-pyrazole 2A A receptor agonists |
USRE47351E1 (en) | 1999-06-22 | 2019-04-16 | Gilead Sciences, Inc. | 2-(N-pyrazolo)adenosines with application as adenosine A2A receptor agonists |
US6403567B1 (en) | 1999-06-22 | 2002-06-11 | Cv Therapeutics, Inc. | N-pyrazole A2A adenosine receptor agonists |
US6605597B1 (en) * | 1999-12-03 | 2003-08-12 | Cv Therapeutics, Inc. | Partial or full A1agonists-N-6 heterocyclic 5′-thio substituted adenosine derivatives |
AU4138601A (en) * | 1999-12-03 | 2001-06-12 | Cv Therapeutics, Inc. | Method of identifying partial adenosine a1 receptor agonists and their use in the treatment of arrhythmias |
US20020012946A1 (en) | 2000-02-23 | 2002-01-31 | Luiz Belardinelli | Method of identifying partial agonists of the A2A receptor |
US6995148B2 (en) * | 2001-04-05 | 2006-02-07 | University Of Pittsburgh | Adenosine cyclic ketals: novel adenosine analogues for pharmacotherapy |
JP2005527502A (en) * | 2002-02-19 | 2005-09-15 | シーブイ・セラピューティクス・インコーポレイテッド | Partial and total agonists of the A1 adenosine receptor |
US8470801B2 (en) | 2002-07-29 | 2013-06-25 | Gilead Sciences, Inc. | Myocardial perfusion imaging methods and compositions |
US20050020915A1 (en) * | 2002-07-29 | 2005-01-27 | Cv Therapeutics, Inc. | Myocardial perfusion imaging methods and compositions |
NZ537975A (en) * | 2002-07-29 | 2007-08-31 | Cv Therapeutics Inc | Method of producing coronary vasodilation without peripheral vasodilation comprising administering at least 10 mcg of at least one A2A receptor agonist |
CA2504929C (en) | 2002-11-05 | 2014-07-22 | Charles Allerson | Compositions comprising alternating 2'-modified nucleosides for use in gene modulation |
US9150606B2 (en) | 2002-11-05 | 2015-10-06 | Isis Pharmaceuticals, Inc. | Compositions comprising alternating 2'-modified nucleosides for use in gene modulation |
US7696345B2 (en) * | 2002-11-05 | 2010-04-13 | Isis Pharmaceuticals, Inc. | Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation |
US9150605B2 (en) | 2002-11-05 | 2015-10-06 | Isis Pharmaceuticals, Inc. | Compositions comprising alternating 2′-modified nucleosides for use in gene modulation |
US20070161582A1 (en) * | 2003-08-08 | 2007-07-12 | Dusan Mijikovic | Pharmaceutical compositions and methods for metabolic modulation |
ATE551339T1 (en) * | 2003-11-05 | 2012-04-15 | Sarcode Bioscience Inc | MODULATORS OF CELLULAR ADHESION |
US8569474B2 (en) | 2004-03-09 | 2013-10-29 | Isis Pharmaceuticals, Inc. | Double stranded constructs comprising one or more short strands hybridized to a longer strand |
US8394947B2 (en) | 2004-06-03 | 2013-03-12 | Isis Pharmaceuticals, Inc. | Positionally modified siRNA constructs |
US7884086B2 (en) | 2004-09-08 | 2011-02-08 | Isis Pharmaceuticals, Inc. | Conjugates for use in hepatocyte free uptake assays |
JP4932488B2 (en) * | 2004-09-17 | 2012-05-16 | キッセイ薬品工業株式会社 | 8-position-modified purine nucleoside derivative and pharmaceutical use thereof |
CN101076343A (en) | 2004-10-20 | 2007-11-21 | Cv医药有限公司 | Use of A2A adenosine receptor agonists |
JP5794721B2 (en) | 2005-05-17 | 2015-10-14 | サーコード バイオサイエンス インコーポレイテッド | Compositions and methods for the treatment of ocular disorders |
US7732595B2 (en) | 2006-02-03 | 2010-06-08 | Gilead Palo Alto, Inc. | Process for preparing an A2A-adenosine receptor agonist and its polymorphs |
MX2008016254A (en) * | 2006-06-22 | 2009-01-15 | Cv Therapeutics Inc | Use of a2a adenosine receptor agonists in the treatment of ischemia. |
KR20090047499A (en) * | 2006-09-01 | 2009-05-12 | 씨브이 쎄러퓨틱스, 인코포레이티드 | Methods and compositions for increasing patient tolerability during myocardial imaging methods |
US20090081120A1 (en) * | 2006-09-01 | 2009-03-26 | Cv Therapeutics, Inc. | Methods and Compositions for Increasing Patient Tolerability During Myocardial Imaging Methods |
JP2011502101A (en) * | 2006-09-29 | 2011-01-20 | ギリアード・パロ・アルト・インコーポレイテッド | Myocardial imaging in patients with a history of lung disease |
WO2008086096A2 (en) * | 2007-01-03 | 2008-07-17 | Cv Therapeutics, Inc. | Myocardial perfusion imaging |
EP2209371B1 (en) | 2007-10-19 | 2017-01-04 | SARcode Bioscience Inc. | Compositions and methods for treatment of diabetic retinopathy |
US8080562B2 (en) * | 2008-04-15 | 2011-12-20 | Sarcode Bioscience Inc. | Crystalline pharmaceutical and methods of preparation and use thereof |
JP2011516607A (en) * | 2008-04-15 | 2011-05-26 | サーコード コーポレイション | Delivery of LFA-1 antagonists to the gastrointestinal system |
US20090258070A1 (en) * | 2008-04-15 | 2009-10-15 | John Burnier | Topical LFA-1 antagonists for use in localized treatment of immune related disorders |
EP2265124A4 (en) * | 2008-04-15 | 2011-12-28 | Sarcode Bioscience Inc | Aerosolized lfa-1 antagonists for use in localized treatment of immune related disorders |
CA2737077A1 (en) * | 2008-09-29 | 2010-04-01 | Gilead Sciences, Inc. | Combinations of a rate control agent and an a-2-alpha receptor antagonist for use in multidetector computed tomography methods |
WO2011050175A1 (en) | 2009-10-21 | 2011-04-28 | Sarcode Corporation | Crystalline pharmaceutical and methods of preparation and use thereof |
CN110922393A (en) | 2012-07-25 | 2020-03-27 | 诺华股份有限公司 | LFA-1 inhibitors and polymorphs thereof |
CZ308881B6 (en) | 2014-12-09 | 2021-08-04 | Univerzita Palackého v Olomouci | 6-aryl-9-glycosylpurines and their use |
GB201820685D0 (en) * | 2018-12-19 | 2019-01-30 | NuCana plc | Synthesis of 8-chloroadenosine derivatives |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2881164A (en) * | 1955-07-18 | 1959-04-07 | American Cyanamid Co | Ribofuranosyl derivatives of 6-aralkylamino and 6-heterocyclicalkylaminopurines |
US3150124A (en) * | 1962-03-16 | 1964-09-22 | Svarnas George | Synthesis of kinetin glycosides |
JPS57171998A (en) * | 1981-04-15 | 1982-10-22 | Fujisawa Pharmaceut Co Ltd | Adenosine derivative and its salt, preparation thereof and medicinal composition containing the same |
US4616003A (en) * | 1984-10-26 | 1986-10-07 | Warner-Lambert Company | N6 -dihydroxypropyladenosines |
US4755594A (en) * | 1986-01-31 | 1988-07-05 | Warner-Lambert Company | N6 -substituted adenosines |
GB8729994D0 (en) * | 1987-12-23 | 1988-02-03 | Glaxo Group Ltd | Chemical compounds |
US5055569A (en) * | 1989-10-19 | 1991-10-08 | G. D. Searle & Co. | N-(6)-substituted adenosine compounds |
US5236902A (en) * | 1990-11-26 | 1993-08-17 | The Governors Of The University Of Alberta | Method and probes for detecting nucleoside transporter and method for producing the probes |
US5432164A (en) * | 1991-10-24 | 1995-07-11 | Novo Nordisk A/S | C2,N6 -disubstituted adenosine derivatives |
AU4226693A (en) * | 1992-05-01 | 1993-11-29 | United States Of America, Represented By The Secretary, Department Of Health And Human Services, The | Phosphorothioate derivatives of cyclic AMP analogues |
US5683989A (en) * | 1993-12-17 | 1997-11-04 | Novo Nordisk A/S | Treatment of ischemias by administration of 2,N6 -substituted adenosines |
JPH07324035A (en) * | 1994-05-30 | 1995-12-12 | L T T Kenkyusho:Kk | Agent for suppressing proliferation of synoviocyte and agent for treatment of rheumatoid arthritis |
KR100254106B1 (en) | 1994-06-09 | 2000-05-01 | 타이도 나오카타 | 4-quinolinine derivative or salt thereof |
US5789416B1 (en) * | 1996-08-27 | 1999-10-05 | Cv Therapeutics Inc | N6 mono heterocyclic substituted adenosine derivatives |
GB9723566D0 (en) * | 1997-11-08 | 1998-01-07 | Glaxo Group Ltd | Chemical compounds |
-
1999
- 1999-12-03 US US09/454,485 patent/US6294522B1/en not_active Expired - Lifetime
-
2000
- 2000-11-29 NZ NZ518736A patent/NZ518736A/en unknown
- 2000-11-29 AU AU45101/01A patent/AU771242B2/en not_active Ceased
- 2000-11-29 WO PCT/US2000/042396 patent/WO2001042265A2/en active IP Right Grant
- 2000-11-29 CA CA002394861A patent/CA2394861C/en not_active Expired - Fee Related
- 2000-11-29 AT AT00992554T patent/ATE292140T1/en not_active IP Right Cessation
- 2000-11-29 IL IL14965100A patent/IL149651A0/en active IP Right Grant
- 2000-11-29 TR TR2002/01470T patent/TR200201470T2/en unknown
- 2000-11-29 MX MXPA02005318A patent/MXPA02005318A/en active IP Right Grant
- 2000-11-29 EP EP00992554A patent/EP1237898B1/en not_active Expired - Lifetime
- 2000-11-29 JP JP2001543562A patent/JP4021196B2/en not_active Expired - Fee Related
- 2000-11-29 CN CNB008166269A patent/CN1195772C/en not_active Expired - Fee Related
- 2000-11-29 DE DE60019164T patent/DE60019164T2/en not_active Expired - Lifetime
- 2000-11-29 KR KR10-2002-7007096A patent/KR100484987B1/en not_active IP Right Cessation
- 2000-11-29 ES ES00992554T patent/ES2236044T3/en not_active Expired - Lifetime
- 2000-12-01 AR ARP000106396A patent/AR029198A1/en active IP Right Grant
- 2000-12-02 TW TW089125685A patent/TWI268923B/en not_active IP Right Cessation
-
2001
- 2001-06-14 US US09/881,281 patent/US20020045595A1/en not_active Abandoned
-
2002
- 2002-05-14 IL IL149651A patent/IL149651A/en not_active IP Right Cessation
- 2002-05-30 NO NO20022573A patent/NO323138B1/en not_active IP Right Cessation
- 2002-05-31 ZA ZA200204379A patent/ZA200204379B/en unknown
-
2003
- 2003-02-28 HK HK03101536.6A patent/HK1051695B/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU4510101A (en) | 2001-06-18 |
KR20030032906A (en) | 2003-04-26 |
DE60019164T2 (en) | 2006-02-02 |
EP1237898B1 (en) | 2005-03-30 |
CN1195772C (en) | 2005-04-06 |
WO2001042265A2 (en) | 2001-06-14 |
ATE292140T1 (en) | 2005-04-15 |
AU771242B2 (en) | 2004-03-18 |
TWI268923B (en) | 2006-12-21 |
JP2003516413A (en) | 2003-05-13 |
WO2001042265A3 (en) | 2002-01-03 |
CA2394861A1 (en) | 2001-06-14 |
CN1402732A (en) | 2003-03-12 |
ES2236044T3 (en) | 2005-07-16 |
NZ518736A (en) | 2004-02-27 |
IL149651A (en) | 2006-08-20 |
US6294522B1 (en) | 2001-09-25 |
TR200201470T2 (en) | 2002-12-23 |
ZA200204379B (en) | 2003-06-25 |
AR029198A1 (en) | 2003-06-18 |
MXPA02005318A (en) | 2002-12-11 |
CA2394861C (en) | 2007-01-30 |
JP4021196B2 (en) | 2007-12-12 |
NO20022573D0 (en) | 2002-05-30 |
NO20022573L (en) | 2002-05-30 |
KR100484987B1 (en) | 2005-04-22 |
HK1051695A1 (en) | 2003-08-15 |
NO323138B1 (en) | 2007-01-08 |
IL149651A0 (en) | 2002-11-10 |
HK1051695B (en) | 2005-06-03 |
DE60019164D1 (en) | 2005-05-04 |
EP1237898A2 (en) | 2002-09-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6294522B1 (en) | N6 heterocyclic 8-modified adenosine derivatives | |
US6605597B1 (en) | Partial or full A1agonists-N-6 heterocyclic 5′-thio substituted adenosine derivatives | |
EP1192169B1 (en) | C-pyrazole a2a receptor agonists | |
US6258793B1 (en) | N6 heterocyclic 5′ modified adenosine derivatives | |
EP1192170B1 (en) | Propargyl phenyl ether a2a receptor agonists | |
US6576620B2 (en) | Method of identifying partial adenosine A1 receptor agonists | |
US6440948B1 (en) | Thiophene A2A receptor agonists |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |