|Número de publicación||US20040234575 A1|
|Tipo de publicación||Solicitud|
|Número de solicitud||US 10/483,545|
|Número de PCT||PCT/DE2003/001254|
|Fecha de publicación||25 Nov 2004|
|Fecha de presentación||15 Abr 2003|
|Fecha de prioridad||9 May 2002|
|También publicado como||CA2484269A1, CA2484269C, CA2484374A1, CA2484374C, CN1318103C, CN1543362A, CN1665554A, CN1665554B, DE10393059D2, DE10393060D2, DE50305580D1, DE50310322D1, EP1501565A1, EP1501565B1, EP1501566A1, EP1501566B1, US8784862, US20050176678, US20110009955, WO2003094990A1, WO2003094991A1|
|Número de publicación||10483545, 483545, PCT/2003/1254, PCT/DE/2003/001254, PCT/DE/2003/01254, PCT/DE/3/001254, PCT/DE/3/01254, PCT/DE2003/001254, PCT/DE2003/01254, PCT/DE2003001254, PCT/DE200301254, PCT/DE3/001254, PCT/DE3/01254, PCT/DE3001254, PCT/DE301254, US 2004/0234575 A1, US 2004/234575 A1, US 20040234575 A1, US 20040234575A1, US 2004234575 A1, US 2004234575A1, US-A1-20040234575, US-A1-2004234575, US2004/0234575A1, US2004/234575A1, US20040234575 A1, US20040234575A1, US2004234575 A1, US2004234575A1|
|Inventores||Roland Horres, Marita Linssen, Michael Hoffmann, Erika Hoffmann, Donato Di Biase, Volker Faust|
|Cesionario original||Roland Horres, Linssen Marita Katarina, Michael Hoffmann, Erika Hoffmann, Donato Di Biase, Volker Faust|
|Exportar cita||BiBTeX, EndNote, RefMan|
|Citas de patentes (24), Otras citas (6), Citada por (33), Clasificaciones (49), Eventos legales (3)|
|Enlaces externos: USPTO, Cesión de USPTO, Espacenet|
 The invention concerns the utilisation of polysaccharides containing the sugar building block N-acylglucosamine for the preparation of hemocompatible surfaces of medical devices, methods for the hemocompatible coating of surfaces with said polysaccharides as well as medical devices with these hemocompatible surfaces.
 In the human body the blood gets only in cases of injuries in contact with surfaces other than the inside of natural blood vessels. Consequently the blood coagulation system gets always activated to reduce the bleeding and to prevent a life-threatening loss of blood, if blood gets in contact with foreign surfaces. Due to the fact that an implant also represents a foreign surface, all patients, who receive an implant, which is in permanent contact with blood, are treated for the duration of the blood contact with drugs, so called anticoagulants, that suppress the blood coagulation, so that considerable side effects have to be taken in account.
 Whilst the usage of vessel supports, so-called stents, the described risk of thrombosis also occurs as one of the risk factors in blood bearing vessels. In cases of vessel strictures and sealings due to e.g. arteriosclerotic changes especially of the coronary arteries the stent is used for the expansion of the vessel walls. It fixes lime fragments in the vessels and improves the flow properties of the blood inside the vessel as it smoothens the surface of the interior space of the vessel. Additionally a stent leads to a resistance against elastic restoring forces of the expanded vessel part. The utilised material is mostly medicinal stainless steel.
 The stent thrombosis occurs in less than one percent of the cases already in the cardio catheter laboratory as early thrombosis or in two to five percent of the cases during the hospital recreation. In about five percent of the cases vessel injuries due to the intervention are caused because of the arterial lock and the possibility of causing pseudo-aneurysms by the expansion of vessels exists, too. Additionally the continuous application of heparin as anticoagulant increases the risk of bleeding.
 An additional and very often occuring complication is restenosis, the resealing of the vessel. Although stents minimise the risk of a renewed sealing of the vessel they are until now not totally capable of hindering the restenosis. The rate of resealing (restenosis) after implantation of a stent is with up to 30% one of the main reasons of a repeated hospital visit for the patients.
 An exact conceptual description of the restenosis does not exist in the professional literature. The mostly used morphologic definition of the restenosis is that after a successful PTA (percutaneous transluminal angioplasty) the restenosis is defined as a reduction of the vessel diameter to less than 50% of the normal one. This is an empirically defined value of which the hemodynamic relevance and its relation to clinical symptomatics lacks of a massive scientific basis. In praxis the clinical aggravation of the patient is often viewed as a sign for a restenosis of the formerly treated vessel part.
 The vessel injuries caused during the implantation of the stents arise inflammation reactions, which play an important role for the healing process during the first seven days. The herein concurrent processes are among others connected with the release of growth factors, which initiate an increased proliferation of the smooth muscle cells and lead with this to a rapid restenosis, a renewed sealing of the vessel because of uncontrolled growth. Even after a couple of weeks, when the stent is grown into the tissue of the blood vessel and totally surrounded by smooth muscle cells, cicatrisations can be too distinctive (neointima hyperplasia) and lead to not only a coverage of the stent surface but to the sealing of the total interior space of the stent.
 It was tried vainly to solve the problem of restenosis by the coating of the stents with heparin (J. Whörle et al., European Heart Journal (2001) 22, 1808-1816). Heparin addresses as anticoagulant only the first mentioned cause and is moreover able to unfold its total effect only in solution. This first problem is meanwhile almost totally avoidable medicamentously by application of anticoagulants. The further problem is intended to be solved now by inhibiting the growth of the smooth muscle cells locally on the stent. This is carried out by e.g. radioactive stents or stents, which contain pharmaceutical active agents.
 Consequently there is a demand on non-thrombogeneous, hemocompatible materials, which are not detected as foreign surface and in case of blood contact does not activate the coagulation system and lead to the coagulation of the blood, with which an important factor for the restenosis stimulating processes is eliminated. Support is supposed to be guaranteed by addition of active agents which shall suppress the inflammation reactions or which shall control the healing process accompanying cell division.
 The undertakings are enormous on this area of producing a stent which can reduce the restenosis in this manner or eliminate totally. Herein different possibilities of realisation are examined in numerous studies. The most common construction type consists of a stent, which is coated with a suitable matrix, usually a biostable polymer. The matrix includes an antiproliferative or antiphlogistic agent, which is released in temporally controlled steps and shall suppress the inflammation reactions and the excessive cell division.
 U.S. Pat. No. 5,891,108 reveals for example a hollow moulded stent, which can contain pharmaceutical active agents in its interior, that can be released throughout a various number of outlets in the stent. Whereas EP-A-1 127 582 describes a stent that shows on its surface ditches of 0.1-1 mm depth and 7-15 mm length, which are suitable for the implementation of an active agent. These active agent reservoirs release, similarly to the outlets in the hollow stent, the contained pharmaceutical active agent in a punctually high concentration and over a relatively long period of time, which leads to the fact, that the smooth muscle cells are not anymore or only very delayed capable of enclosing the stent. As a consequence the stent is much longer exposed to the blood, what leads again to increased vessel sealings by thrombosis (Liistro F., Colombo A., Late acute thrombosis after paclitaxel eluting stent implantation. Heart (2001) 86 262-4).
 One approach to this problem is represented by the phosphorylcholine coating of Biocompatibles (WO 0101957), as here phosphorylcholine, a component of the erythrocytic cell membrane, shall create a non thrombogeneous surface as ingredient of the coated non biodegredable polymer layer on the stent. Dependent of its molecular weight the active agent is absorbed by the polymer containing phosphorylcholine layer or adsorbed on the surface.
 Object of the present invention is to provide hemocompatibly coated medical devices as well as methods of hemocompatible coating and the use of hemocompatibly coated medical devices, especially stents, to prevent or reduce undesired reactions as for example restenosis.
 Especially object of the present invention is to provide stents which permit a continuous controlled ingrowth of the stent—on the one side by suppression of the cellular reactions in the primal days and weeks after implantation by the support of the selected agents and agent combinations and on the other side by providing an athrombogeneous resp. inert resp. biocompatible surface, which guarantees that with the decrease of the agent's influence no reactions to the existing foreign surface take place which also can lead to complications in a long term.
 The intentions of creating a nearly perfect simulation of the native athrombogeneous conditions of that part of a blood vessel that is allocated on the blood side are enormous. EP-B-0 333 730 describes a process to produce hemocompatible substrates by recess, adhesion and/or modification and anchorage of non thrombogeneous endothelic cell surface polysaccharide (HS I). The immobilisation of this specific endothelic cell surface proteoheparane sulphate HS I on biological or artificial surfaces effects that suchlike coated surfaces get blood compatible and suitable for the permanent blood contact. A disadvantage whereas is, that this process for the preparation of HS I premises the cultivation of endothelic cells, so that the economical suitability of this process is strongly limited, because the cultivation of endothelic cells is time taking and greater amounts of cultivated endothelic cells are only obtainable with immense expenditure.
 The present invention solves the object by providing medical devices that show properties of a surface coating of determined polysaccharides and paclitaxel. Instead of or together with paclitaxel determined other antiphlogistic as well as anti-inflammatory drugs resp. agent combinations of simvastatine, 2-methylthiazolidine-2,4-dicarboxylic acid and the correspondent sodium salt), macrocyclic suboxide (MCS) and its derivatives, tyrphostines, D24851, thymosin a-1, interleucine-1β inhibitors, activated protein C (aPC), MSH, fumaric acid and fumaric acid ester, PETN (pentaerythritol tetranitrate), PI88, dermicidin, baccatin and its derivatives, docetaxel and further derivatives of paclitaxel, tacrolimus, pimecrolimus, trapidil, a- and β-estradiol, sirolimus, colchicin, and melanocyte-stimulating hormone (α-MSH) can be used. Methods for the production of these hemocompatible surfaces are given in the claims 20-31. Preferred embodiments can be found in the dependent claims, the examples as well as the figures.
 The subject matter of the present invention are medical devices the surface of which is at least partially covered with a hemocompatible layer, wherein the hemocompatible layer comprises at least one compound of the formula 1:
 n is an integer between 4 and 1050 and
 Y represents the residues —CHO, —COCH3, —COC2H5, —COC3H7, —COC4H9, —COC5H11, —COCH(CH3)2, —COCH2CH(CH3)2, —COCH(CH3)C2H5, —COC(CH3)3, —CH2COO−, —C2H4COO−, —C3H6COO−, —C4H8COO−.
 It is also possible to use any salts of the compounds of formula 1. The hemocompatible layer can be added directly onto the surface of a preferably non hemocompatible medical device or deposited onto other biostable and/or biodegradable layers. Further on additional biostable and/or biodegradable and/or hemocompatible layers can be localised on the hemocompatible layer. In addition to this the active agent paclitaxel is present on, in and/or under the hemocompatible layer or the hemocompatible layers, respectively. The active agent (paclitaxel) can form herein an own active agent layer on or under the hemocompatible layer and/or can be incorporated in at least one of the biostable, biodegradable and/or hemocompatible layers. Preferably the compounds of the general formula 1 are used, wherein Y is one of the following groups: —CHO, —COCH3, —COC2H5 or —COC3H7. Further on preferred are the groups —CHO, —COCH3, —COC2H5 and especially preferred is the group —COCH3.
 The compounds of the general formula 1 contain only a small amount of free amino groups. Because of the fact that with the ninhydrine reaction free amino groups could not be detected anymore, due to the sensitivity of this test it can be implied that less than 2%, preferred less than 1% and especially preferred less than 0.5% of all —NH—Y groups are present as free amino groups, i.e. within this low percentage of the —NH—Y groups Y represents hydrogen.
 Because polysaccharides of the general formula 1 contain carboxylate groups and amino groups, the general formula covers alkali as well as alkaline earth metal salts of the corresponding polysaccharides. Alkali metal salts like the sodium salt, the potassium salt, the lithium salt or alkaline earth metal salts like the magnesium salt or the calcium salt can be mentioned. Further on with ammonia, primary, secondary, tertiary and quaternary amines, pyridine and pyridine derivatives ammonium salts, preferably alkylammonium salts and pyridinium salts can be formed. Among the bases, which form salts with the polysaccharides, are inorganic and organic bases as for example NaOH, KOH, LiOH, CaCO3, Fe(OH)3, NH4OH, tetraalkylammonium hydroxide and similar compounds.
 The polysaccharides according to formula 1 possess molecular weights from 2 kD to 15 kD, preferred from 4 kD to 13 kD, more preferred from 6 kD to 12 kD and especially preferred from 8 kD to 11 kD. The variable n is an integer in the range of 4 to 1050. Preferred n is an integer from 9 to 400, more preferred an integer from 14 to 260 and especially preferred an integer between 19 and 210.
 The general formula 1 shows a disaccharide, which has to be viewed as the basic module for the used polysaccharides and that formes the polysaccharide by the n-fold (multiple) sequencing of the basic module. This basic module which is built of two sugar molecules shall not be interpreted in the manner, that the general formula 1 only includes polysaccharides with an even number of sugar molecules. The formula implements of course also polysaccharides with an odd number of sugar building units. The end groups of the polysaccharides are represented by hydroxyl groups.
 Especially preferred are medical devices which contain immediately on the surface of the medical device a hemocompatible layer consisting of the compounds according to formula 1 and above it a layer of paclitaxel. The paclitaxel layer can diffuse partially into the hemocompatible layer or get taken up totally by the hemocompatible layer.
 It is further preferred, if at least one biostable layer is present under the hemocompatible layer. In addition the hemocompatible layer can be coated totally and/or partially with at least one more, above lying biostable and/or biodegradable layer. Preferred is an external biodegradable or hemocompatible layer.
 A further preferred embodiment contains a layer of paclitaxel under the hemocompatible layer or between the biostable and the hemocompatible layer, so that paclitaxel is released slowly through the hemocompatible layer. Paclitaxel can be bound covalently and/or adhesively in and/or on the hemocompatible layer and/or the biostable and/or the biodegradable layer, in which the adhesive bonding is preferred.
 As biodegradable substances for the biodegradable layer(s) can be used: polyvalerolactones, poly-ε-decalactones, polylactonic acid, polyglycolic acid, polylactides, polyglycolides, copolymers of the polylactides and polyglycolides, poly-ε-caprolactone, polyhydroxybutanoic acid, polyhydroxybutyrates, polyhydroxyvalerates, polyhydroxybutyrate-co-valerates, poly(1,4-dioxane-2,3-diones), poly(1,3-dioxane-2-one), poly-para-dioxanones, polyanhydrides such as polymaleic anhydrides, polyhydroxymethacrylates, fibrin, polycyanoacrylates, polycaprolactonedimethylacrylates, poly-b-maleic acid, polycaprolactonebutyl-acrylates, multiblock polymers such as e.g. from oligocaprolactonedioles and oligodioxanonedioles, polyether ester multiblock polymers such as e.g. PEG and poly(butyleneterephtalates), polypivotolactones, polyglycolic acid trimethyl-carbonates, polycaprolactone-glycolides, poly(g-ethylglutamate), poly(DTH-iminocarbonate), poly(DTE-co-DT-carbonate), poly(bisphenol-A-iminocarbonate), polyorthoesters, polyglycolic acid trimethyl-carbonates, polytrimethylcarbonates, polyiminocarbonates, poly(N-vinyl)-pyrrolidone, polyvinylalcoholes, polyesteramides, glycolated polyesters, polyphosphoesters, polyphosphazenes, poly[p-carboxyphenoxy)propane], polyhydroxypentanoic acid, polyanhydrides, polyethyleneoxide-propyleneoxide, soft polyurethanes, polyurethanes with amino acid residues in the backbone, polyether esters such as polyethyleneoxide, polyalkeneoxalates, polyorthoesters as well as their copolymers, lipides, carrageenans, fibrinogen, starch, collagen, protein based polymers, polyamino acids, synthetic polyamino acids, zein, modified zein, polyhydroxyalkanoates, pectic acid, actinic acid, modified and non modified fibrin and casein, carboxymethylsulphate, albumin, moreover hyaluronic acid, chitosan and its derivatives, heparansulphates and its derivatives, heparins, chondroitinsulphate, dextran, b-cyclodextrins, copolymers with PEG and polypropyleneglycol, gummi arabicum, guar, gelatine, collagen, collagen-N-hydroxysuccinimide, lipids, phospholipids, modifications and copolymers and/or mixtures of the afore mentioned substances.
 As biostable substances for the biostable layer(s) can be used: polyacrylic acid and polyacrylates as polymethylmethacrylate, polybutylmethacrylate, polyacrylamide, polyacrylonitriles, polyamides, polyetheramides, polyethylenamine, polyimides, polycarbonates, polycarbourethanes, polyvinylketones, polyvinylhalogenides, polyvinylidenhalogenides, polyvinylethers, polyisobutylenes, polyvinylaromates, polyvinylesters, polyvinylpyrollidones, polyoxymethylenes, polytetramethyleneoxide, polyethylene, polypropylene, polytetrafluoroethylene, polyurethanes, polyetherurethanes, silicone-polyetherurethanes, silicone-polyurethanes, silicone-polycarbonate-urethanes, polyolefine elastomeres, polyisobutylenes, EPDM gums, fluorosilicones, carboxymethylchitosanes, polyaryletheretherketones, polyetheretherketones, polyethylenterephthalate, polyvalerates, carboxymethylcellulose, cellulose, rayon, rayontriacetates, cellulosenitrates, celluloseacetates, hydroxyethylcellulose, cellulosebutyrates, celluloseacetatebutyrates, ethylvinylacetate copolymers, polysulphones, epoxy resins, ABS resins, EPDM gums, silicones as polysiloxanes, polydimethylsiloxanes, polyvinylhalogenes and copolymers, celluloseethers, cellulosetriacetates, chitosanes and copolymers and/or mixtures of these substances.
 It is possible to furnish any medical devices with the herein disclosed hemocompatible surfaces, especially those, which shall be suitable for the short- or the longterm contact with blood or blood products. Such medical devices are for example prostheses, organs, vessels, aortas, heart valves, tubes, organ spareparts, implants, fibers, hollow fibers, stents, hollow needles, syringes, membranes, tinned goods, blood containers, titrimetric plates, pacemakers, adsorbing media, chromatography media, chromatography columns, dialyzers, connexion parts, sensors, valves, centrifugal chambers, recuperators, endoscopes, filters, pump chambers. The present invention is especially related to stents.
 The polysaccharides of formula 1 can be formed from heparin and/or heparansulphates. These materials are in structurally view quite similar compounds. Heparansulphates occur ubiquitously on cell surfaces of mammals. In dependence from the cell type they differ strongly in molecular weight, degree of acetylation and degree of sulphation. Heparansulphate from liver shows for example an acetylation coefficient of about 50%, whereas the heparansulphate of the glycocalix from endothelic cells can exhibit an acetylation coefficient from about 90% and higher. Heparin shows only a quite low degree of acetylation from about up to 5%. The sulphation coefficient of the heparansulphate from liver and of heparin is ˜2 per disaccharide unit, in case of heparansulphate from endothelial cells close to 0 and in heparansulphates from other cell types between 0 and 2 per disaccharide unit.
 The compounds of the general formula 1 are characterized by an amount of sulphate groups per disaccharide unit of less than 0.05. Further on the amount of free amino groups in these compounds is less than 1% based on all —NH—Y groups.
 The following image shows a tetrasaccharide unit of a heparin or a heparansulphate with random orientation of the sulphate groups and with a sulphation coefficient of 2 per disaccharide unit as it is typical for heparin:
 All heparansulphates have with heparin a common sequence in biosynthesis. First of all the core protein with the xylose-containing bonding region is formed. It consists of the xylose and two galactose residues connected to it. To the last of the two galactose units a glucuronic acid and a galactosamine is connected alternately until the adequate chain length is reached. Finally, a several step enzymatic modification of this common polysaccharide precursor of all heparansulphates and of heparin follows by means of sulphotransferases and epimerases which generate by their varying completeness of transformation the broad spectra of different heparansulphates up to heparin.
 Heparin is alternately build of D-glucosamine and D-glucuronic acid resp. L-iduronic acid, in which the amount of L-iduronic acid is up to 75%. D-glucosamine and D-glucuronic acid are connected in a β-1,4-glycosidic resp. L-iduronic acid in an a-1,4-glycosidic bonding to the disaccharide, that forms the heparin subunits. These subunits are again connected to each other in a β-1,4-glycosidic way and lead to heparin. The position of the sulphonyl groups is variable. In average one tetrasaccharide unit contains 4 to 5 sulphuric acid groups. Heparansulphate, also named as heparitinsulphate, contains with exception of the heparansulphate from liver less N- and O-bound sulphonyl goups as heparin but in exchange more N-acetyl goups. The amount of L-iduronic acid compared to heparin is also lower.
 As it is evident from FIG. 1 the compounds of the general formula (cf. FIG. 1b as example) are structurally similar to the natural heparansulphate of endothelial cells, but avoid the initially mentioned disadvantages by the use of endothelial cell heparan sulphates.
 For the antithrombotic activity a special pentasaccharide unit is made responsible, which can be found in commercial heparin preparatives in about every 3rd molecule. Heparin preparations of different antithrombotic activity can be produced by special separation techniques. In highly active, for example by antithrombin-III-affinitychromatography obtained preparations (“High-affinity”-heparin) this active sequence is found in every heparin molecule, while in “No-affinity”-preparations no characteristical pentasaccharide sequences and thus no active inhibition of coagulation can be detected. Via interaction with this pentasaccharide the activity of antithrombin III, an inhibitor of the coagulation key factor thrombin, is essentially exponentiated (bonding affinity increase up to the factor 2×103) [Stiekema J. C. J.; Clin Nephrology 26, Suppl. Nr 1, S3-S8, (1986)].
 The amino groups of the heparin are mostly N-sulphated or N-acetylated. The most important O-sulphation positions are the C2 in the iduronic acid as well as the C6 and the C3 in the glucosamine. For the activity of the pentasaccharide onto the plasmatic coagulation basically the sulphate group on C6 is made responsible, in smaller proportion also the other functional groups.
 Surfaces of medicinal implants coated with heparin or heparansulphates are and remain only conditionally hemocompatible by the coating. The heparin or heparansulphate which is added onto the artificial surface loses partially in a drastic measure its antithrombotic activity which is related to a restricted interaction due to steric hindrence of the mentioned pentasaccharide units with antithrombin III. Because of the immobilisation of these polyanionic substances a strong adsorption of plasma protein on the heparinated surface is observed in all cases what eliminates on the one hand the coagulation suppressing effect of heparin resp. of heparansulphates and initialises on the other hand specific coagulation processes by adherent and hereby tertiary structure changing plasma proteins (e.g. albumin, fibrinogen, thrombin) and hereon adherent platelets.
 Thus a correlation exists on the one hand between the limited interaction of the pentasaccharide units with antithrombin III by immobilisation on the other hand depositions of plasma proteins on the heparin-resp. heparansulphate layer on the medicinal implant take place, which leads to the loss(es) of the antithrombotic properties of the coating and which can even turn into the opposite, because the plasma protein adsorption, that occurs during a couple of seconds leads to the loss of the anticoagulational surface and the adhesive plasma proteins change their tertiary structure, whereby the antithrombogenity of the surface turns vice versa and a thrombogenous surface arises. Surprisingly it could be detected, that the compounds of the general formula 1, despite of the structural differences to the heparin resp. heparansulphate, still show the hemocompatible properties of heparin and additionally after the immobilisation of the compounds no noteworthy depositions of plasma proteins, which represent an initial step in the activation of the coagulation cascade, could be observed. The hemocopatible properties of the compounds according to invention still remain also after their immobilisation on artificial surfaces.
 Further on it is supposed that the sulphate groups of the heparin resp. the heparansulphates are necessary for the interaction with antithrombin III and impart thereby the heparin resp. the heparansulphate the anticoagulatory effect. The inventive compounds are not actively coagulation suppressive, i.e. anticoagulative, due to an almost complete desulphation the sulphate groups of the compounds are removed up to a low amount of below 0.2 sulphate groups per disaccharide unit.
 The inventive compounds of the general formula 1 can be generated from heparin or heparansulphates by first substantially complete desulphation of the polysaccharide and subsequently substantially complete N-acylation. The term “substantially completely desulphated” refers to a desulphation degree of above 90%, preferred above 95% und especially preferred above 98%. The desulphation coefficient can be determined according to the so-called ninhydrin test which indicates free amino groups. The desulphation takes place in the way as with DMMB (dimethylmethylene blue) no colour reaction is obtained. This colour test is suitable for the indication of sulphated polysaccharides but its detection limit is not known in technical literature. The desulphation can be carried out for example by pyrolysis of the pyridinium salt in a solvent mixture. Especially a mixture of DMSO, 1,4-dioxane and methanol has proven of value.
 Heparansulphates as well as heparin were desulphated via total hydrolysis and subsequently reacylated. Thereafter the number of sulphate groups per disaccharide unit (S/D) was determined by 13C-NMR. The following table 1 shows these results on the example of heparin and desulphated, reacetylated heparin (Ac-heparin).
TABLE 1 Distribution of functional groups per disaccharide unit on the example of heparin and Ac-heparin as determined by 13C-NMR-measurements. 2-S 6-S 3-S NS N—Ac NH2 S/D Heparin 0.63 0.88 0.05 0.90 0.08 0.02 2.47 Ac-heparin 0.03 0 0 0 1.00 — 0.03
 A sulphate content of about 0.03 sulphate groups/disaccharide unit (S/D) in case of Ac-heparin in comparison with about 2.5 sulphate groups/disaccharide unit in case of heparin was reproducibly obtained.
 As described above the difference in the sulphate contents of heparin resp. heparansulphates has a considerable influence on the activity adverse to antithrombin III and the coagulatory effects of these compounds. These compounds have a content of sulphate groups per disaccharide unit of less than 0.2, preferred less than 0.07, more preferred less than 0.05 and especially preferred less than 0.03 sulphate groups per disaccharide unit.
 By the removal of the sulphate groups of heparin, to which the active coagulation suppressive working mechanism is accredited to, one receives for a surface refinement suitable hemocompatible, coagulation inert oligo-resp. polysaccharide which on the one hand has no active role in the coagulation process and which on the other hand is not detected by the coagulation system as foreign surface. Accordingly this coating imitates successfully the nature given highest standard of hemocompatibility and passivity against the coagulation active components of the blood. The examples 3 and 4 clarify, that surfaces, which are coated with the compounds according to invention, especially are coated covalently, result in a passivative, athrombogeneous and hemocompatible coating. This is definitely proven by the example of Ac-heparins.
 Substantially completely N-acylated refers to a degree of N-acylation of above 94%, preferred above 97% and especially preferred above 98%. The acylation runs in such a way completely that with the ninhydrin reaction for detection of free amino groups no colour reaction is obtained anymore. As acylation agents are preferably used carboxylic acid chlorides, -bromides or -anhydrides. Acetic anhydride, propionic anhydride, butyric anhydride, acetic acid chloride, propionic acid chloride or butyric acid chloride are for example suitable for the synthesis of the compounds according to invention. Especially suitable are carboxylic anhydrides as acylation agents.
 As solvent especially for carboxylic acid anhydrides deionised water is used, especially together with a cosolvent which is added in an amount from 10 to 30 volume percent. As cosolvents are suitable methanol, ethanol, DMSO, DMF, acetone, dioxane, THF, ethyl acetate and other polar solvents. In case of the use of carboxylic acid halogenides preferably polar water free solvents such as DMSO or DMF are used.
 The inventive compounds of the general formula comprise in the half of the sugar molecules a carboxylate group and in the other half a N-acyl group.
 The present invention describes the use of the compounds with the general formula 1 as well as salts of these compounds for the coating, especially a hemocompatible coating of natural and/or artificial surfaces. Under “hemocompatible” the characteristic of the compounds according to invention is meant, not to interact with the compounds of the blood coagulation system or the platelets and so not to initiate the blood coagulation cascade.
 In addition the invention reveals polysaccharides for the hemocompatible coating of surfaces. Preferred are polysaccharides in the range of the above mentioned molecular weight limits. The used polysaccharides are characterised in that they contain the sugar building unit N-acylglucosamine in a great amount. This means that 40 to 60% of the sugar building units are N-acylglucosamine and substantially the remaining sugar building units bear each a carboxyl group. The polysaccharides consist generally in more than 95%, preferred in more than 98%, of only two sugar building units, whereas one sugar building unit bears a carboxyl group and the other one a N-acyl group.
 One sugar building unit of the polysaccharides is N-acylglucosamine preferred N-acetylglucosamine and in case of the other one it is the uronic acids glucuronic acid and iduronic acid. Preferred are polysaccharides, which conspire substantially the sugar glucosamine, whereas substantially the half of the sugar building units bears a N-acyl group, preferred a N-acetyl group, and the other half of the glucosamine building units bears one carboxyl group which is bond directly by the amino group or by one or more methylenyl groups. In the case of these carboxylic acid groups bound to the amino group it is concerned to be preferred the carboxymethyl- or carboxyethyl groups. Furthermore, polysaccharides are preferred which substantially conspire in one half of N-acylglucosamine, preferred of N-acetylglucosamine and substantially conspire in the other half of the uronic acids glucuronic acid and iduronic acid. Especially preferred are the polysaccharides, that show a substantially alternating sequence of N-acylglucosamine and one of the both uronic acids.
 Surprisingly it was shown, that for the applications according to invention especially desulphated and substantially N-acylated heparin is especially suitable. Especially N-acetylated heparin is suitable for the hemocompatible coating.
 The term “substantially” shall make clear, that statistical variations are to be taken into account. One substantially alternating sequence of the sugar building units implies, that generally no two equal sugar building units are bound to each other but does not exclude totally such a defect connection. In accordance “substantially the half” means almost 50% but allows small variations, because especially in the case of biosynthetically synthesised macromolecules the ideal case is never reached and some variations are always to be taken into account, because enzymes do not work perfectly and in catalysis always some error rate has to be anticipated. Whereas in case of natural heparin a strongly alternating sequence of N-acetylglucosamine and the uronic acid units is existing.
 Furthermore, methods for hemocompatible coating of surfaces are disclosed which are especially destined for the direct blood contact. In case of these methods a natural and/or artificial surface is provided and the above described polysaccharides are immobilised on this surface.
 The immobilisation of the polysaccharides on these surfaces can be achieved via hydrophobic interactions, van der Waals forces, electrostatic interactions, hydrogen bonds, ionic interactions, cross-linking of the polysaccharides and/or by covalent bonding onto the surface. Preferred is the covalent linkage of the polysaccharides (side-on bonding), more preferred the covalent single-point linkage (side-on bonding) and especially preferred the covalent end-point linkage (end-on bonding).
 In the following the coating methods according to invention are described.
 Biological and/or artificial surfaces of medical devices can be provided with a hemocompatible coating by means of the following method:
 a) providing a surface of a medical device and
 b) deposition of at least one compound of the general formula 1 according to claim 1 as hemocompatible layer onto this surface and/or
 b′) deposition of a biostable and/or biodegradable layer onto the surface of the medical device or the hemocompatible layer.
 “Deposition” shall refer to at least partial coating of a surface with the adequate compounds, wherein the compounds are positioned and/or immobilised or anyhow anchored on and/or in the subjacent surface.
 Under “substantially the remaining sugar building units” is to be understood that 93% of the remaining sugar building units, preferred 96% and especially preferred 98% of the remaining 60%-40% of the sugar building units bear a carboxyl group.
 An uncoated and/or non hemocompatible surface is preferably provided. “Non hemocompatible” surfaces shall refer to such surfaces that can activate the blood coagulatory system, thus are more or less thrombogeneous.
 An alternative embodiment comprises the steps:
 a) providing surface of a medical device and
 b) deposition of at least one inventive polysaccharide according to formula 1,
 b′) deposition of a biostable layer onto the surface of the medical device and
 d′) deposition of a further hemocompatible layer of at least one inventive polysaccharide according to formula 1.
 The last-mentioned embodiment makes sure, even in the case of e.g. mechanical damage of the polymeric layer and therewith also of the exterior hemocompatible layer, that the surface coating does not lose its characteristic of being blood compatible.
 Under “biological or artificial” surface is the combination of an artificial medical device with an artificial part to be understood, e.g. pork heart with an artificial heart valve.
 The single layers are deposited preferably by dipping or spraying methods, whereas one can deposit also paclitaxel at the same time with the deposition of one layer onto the medical device surface, which is then implemented in the respective layer covalently and/or adhesively bound. In this way it is possible at the same time with the deposition of a hemocompatible layer onto the medical device to deposit the active agent paclitaxel. The substances for the biostable or biodegradable layers were itemised already above.
 Onto this first biostable and/or biodegradable or hemocompatible layer it is then possible in an additional non compulsory step c) to deposit an agent layer of paclitaxel. In a preferred embodiment paclitaxel is bound covalently on the subjacent layer. Also paclitaxel is preferably deposited by dipping or spraying methods on and/or in the hemocompatible layer or the biostable layer.
 After the step b) or the step c) an additional step d) can follow which implements the deposition of at least one biodegradable layer and/or at least one biostable layer onto the hemocompatible layer resp. the layer of paclitaxel.
 According to the alternative embodiments after step b′) or step c) a step d′) can follow which implements the deposition of at least one compound of the general formula 1 as hemocompatible layer onto the biostable and/or biodegradable layer resp. the layer of paclitaxel. Preferably after step b′) the step d′) follows.
 After step d) resp. d′) the deposition of paclitaxel can take place into and/or onto the at least one biodegradable and/or biostable layer or the hemocompatible layer.
 The single layers as well as paclitaxel are preferably deposited and/or implemented by dipping or spraying methods onto and/or into the subjacent layer.
 According to a preferred embodiment the biostable layer is deposited on the surface of the medical device and completely or incompletely covered with a hemocompatible layer which (preferably covalently) is bound to the biostable layer.
 Preferably the hemocompatible layer comprises heparin of native origin of regioselectively synthesised derivatives of different sulphation coefficients (sulphation degrees) and acylation coefficients (acylation degrees) in the molecular weight range of the pentasaccharide, which is responsible for the antithrombotic activity, up to the standard molecular weight of the purchasable heparin of 13 kD, heparansulphate and its derivatives, oligo- and polysaccharides of the erythrocytic glycocalix, desulphated and N-reacetylated heparin, N-carboxymethylated and/or partially N-acetylated chitosan as well as mixtures of these substances.
 Subject of the invention are also medical devices which are hemocompatibly coated according to one of the herein mentioned methods. In the case of the medical devices it is preferably a matter of stents.
 The conventional stents, which can be coated according to the inventive methods, consist of stainless steel, nitinol or other metals and alloys or of synthetic polymers.
 The stents according to invention are coated with an according to the general formula 1 preferred covalently bound hemocompatible layer. A second layer covers this first hemocompatible layer completely or also incompletely. This second layer conspires preferably paclitaxel. The hemocompatible coating of a stent provides on the one hand the necessary blood compatibility and reduces so the risk of thrombosis and also the containment of inflammation reactions due to the intrusion and the absence of a non-endogenous surface, and paclitaxel, which is preferred to be distributed homogeneously over the total surface of the stent provides that the covering of the stent surface with cells, especially smooth muscle and endothelial cells, takes place in a controlled way, so that the interplay of thrombosis reactions and inflammation reactions, the release of growth factors, proliferation and migration of cells during the recovery process provides the generation of a novel “repaired” cell layer, which is referred to as neointima.
 Thus, the use of paclitaxel, covalently or/and adhesively bound to the subjacent layer or/and covalently or/and adhesively implemented in at least one layer, ensures, that this active agent is set free continuously and in small doses, so that the population of the stent surface by cells is not inhibited, however an excessive population and the ingrowth of cells into the vessel lumen is prevented. This combination of both effects awards the ability to the stent according to invention, to grow rapidly into the vessel wall and reduces both the risk of restenosis and the risk of thrombosis. The release of paclitaxel spans about a period from 1 to 12 months, preferably 1 to 3 months after implantation.
 Paclitaxel is preferred contained in a pharmaceutical active concentration from 0.001-10 mg per cm2 stent surface, preferred 0.01-5 mg and especially preferred 0.1-1.0 mg per cm2 stent surface. Additional active agents can be contained in similar concentration in the same or in the hemocompatible layer.
 The applied amounts of polymer are per layer between 0.01 mg to 3 mg, preferred 0.20 mg to 1 mg and especially preferred between 0.2 mg to 0.5 mg. Suchlike coated stents release the active agent paclitaxel controlled and continuously and hence are excellently suitable for the prevention and reduction of restenosis.
 These stents with a hemocompatible coating are generated, as one provides stents and deposits preferred covalently one hemocompatible layer according to the general formula, which masks the surface of the implantate permanently after the release of the active agent and so after the decay of the active agent influence.
 The preferred embodiment of the stents according to invention shows a coating, which consists of at least two layers. Thereby named as second layer is that layer, which is deposited on the first layer. According to the two-layer design the first layer conspires the hemocompatible layer, which is substantially completely covered by a second layer, which consists of paclitaxel, that is covalently and/or adhesively bound to the first layer.
 The paclitaxel layer is dissolved slowly, so that the active agent is released according to the velocity of the solution process. The first hemocompatible layer guarantees the necessary blood compatibility of the stent in the degree as the active agent is removed. By the release of the active agent the adhesion of cells is strongly reduced only for a certain period of time and an aimed controlled adhesion is enabled, where the external layer had been already widely degradated. Finally the hemocompatible layer remains as athrombogeneous surface and masks the foreign surface in such a way, that no life-threatening reaction can occur anymore.
 Suchlike stents can be generated by a method of the hemocompatible coating of stents, to which the following principle underlies:
 a. providing of a stent
 b. deposition of a preferred covalently bound hemocompatible layer
 c. Substantially complete covering of the hemocompatible layer by a dipping or spraying method with the antiproliferative active agent paclitaxel.
 The stents according to invention solve both the problem of acute thrombosis and the problem of neointima hyperplasia after a stent implantation. In addition the inventive stents are especially well suited, because of their coating for the continuous release of one or more antiproliferative, immuno-suppressive active agents. Due to this capability of the aimed continuous active agent release in a required amount the inventively coated stents prevent the danger of restenosis almost completely.
 The natural and/or artificial surfaces which had been coated according to the above described method with a hemocompatible layer of aforesaid polysaccharides, are suitable especially as implants resp. organ replacement parts, that are in direct contact with the blood circuit and blood, preferably in the form of stents in combination with an antiproliferative active agent, preferably paclitaxel, for the prevention of restenosis.
 The inventively coated medical devices are suited especially but not only for the direct and permanent blood contact, but show surprisingly also the characteristic to reduce or even to prevent the adhesion of proteins onto suchlike coated surfaces. The adhesion of plasma proteins on foreign surfaces which come in contact with blood is an essential and initial step for the further events concerning the recognition and the implementing action of the blood system.
 This is for example important in the in vitro diagnostics from body fluids. Thus the deposition of the inventive coating prevents or at least reduces for example the unspecific adhesion of proteins on micro-titer plates or other support mediums which are used for diagnostic detection methods, that disturb the generally sensitive test reactions and can lead to a falsification of the analysis result.
 By use of the coating according to invention on adsorption media or chromatography media the unspecific adhesion of proteins is also prevented or reduced, whereby better separations can be achieved and products of greater purity can be generated.
FIG. 1 shows a tetrasaccharide unit of a heparin or heparansulphate with statistic distribution of the sulphate groups and a sulphation coefficient of 2 per disaccharide unit as it is typical for heparin (FIG. 1a). For comparison of the structural similarities FIG. 1b shows an example of a compound according to the general formula in the description.
FIG. 2 shows the influence of an into a PVC-tube expanded, surface modified stainless steel coronary stent on the platelet loss (PLT-loss).
 An uncoated stainless steel coronary stent was measured as reference. As zero value the level of the platelet loss in case of the PVC-tube without stainless steel coronary stent was set.
 Thereby SH1 is a with heparin covalently coated stent, SH2 is a with chondroitinsulphate coated stent; SH3 is a stent coated with polysaccharides gained from the erythrocytic glycocalix and SH4 is a with Ac-heparin covalently coated stainless steel coronary stent.
FIG. 3 shows a schematic presentation of the restenosis rate of with completely desulphated and N-reacetylated heparin (Ac-heparin) covalently coated stents and with oligo- and polysaccharides of the erythrocytic glycocalix (polysacch. of erythro. glycoc.) coated stents in comparison to the uncoated stent and with polyacrylic acid (PAS) coated stents after 4 weeks of implantation time in pork.
FIG. 4 quantitative coronary angiography:
 Images of the cross sections through the stent containing vessel-segment of one with Ac-heparin coated stent (a.) and as comparison of one uncoated (unco. or bare) stent (b.). After four weeks in the animal experiment (pork) a clear difference in the thicknesses of the formed neointimas can be observed.
FIG. 5 elution plot of paclitaxel from the stent (without support medium).
 Synthesis of Desulphated Reacetylated Heparin:
 100 ml amberlite IR-122 cation exchange resin were added into a column of 2 cm diameter, with 400 ml 3M HCl in the H+-form converted and rinsed with distilled water, until the eluate was free of chloride and pH neutral. 1 g sodium-heparin was dissolved in 10 ml water, added onto the cation exchange column and eluted with 400 ml of water. The eluate was added dropvise into a receiver with 0.7 g pyridine and afterwards titrated with pyridine to pH 6 and freeze-dried.
 0.9 g heparin-pyridinium-salt were added in a round flask with a reflux condenser with 90 ml of a 6/3/1 mixture of DMSO/1,4-dioxan/methanol (v/v/v) and heated for 24 hours to 90° C. Then 823 mg pyridinium chloride were added and heated additional 70 hours to 90° C. Afterwards it was diluted with 100 ml of water and titrated with dilute sodium hyrdoxide to pH 9. The desulphated heparin was dialyzed contra water and freeze-dried.
 100 mg of the desulphated heparin were solved in 10 ml of water, cooled to 0° C. and added with 1.5 ml methanol under stirring. To this solution were added 4 ml dowex 1×4 anion exchange resin in the OH−-form and afterwards 150 μl of acetic anhydride and stirred for 2 hours at 4° C. Then the resin was removed by filtration and the solution was dialyzed contra water and freeze-dried.
 Synthesis of Desulphated N-Propionylated Heparin:
 100 ml amberlite IR-122 cation exchange resin were added into a column of 2 cm diameter, with 400 ml 3M HCl in the H+-form converted and rinsed with distilled water, until the eluate was free of chloride and pH neutral. 1 g sodium-heparin was dissolved in 10 ml water, added onto the cation exchange column and eluted with 400 ml of water. The eluate was added dropvise into a receiver with 0.7 g pyridine and afterwards titrated with pyridine to pH 6 and freeze-dried.
 0.9 g heparin-pyridinium-salt were added in a round flask with a reflux condenser with 90 ml of a 6/3/1 mixture of DMSO/1,4-dioxan/methanol (v/v/v) and heated for 24 hours to 90° C. Then 823 mg pyridiniumchloride were added and heated additional 70 hours to 90° C. Afterwards it was diluted with 100 ml of water and titrated with dilute sodium hydroxide to pH 9. The desulphated heparin was dialyzed contra water and freeze-dried.
 100 mg of the desulphated heparin were solved in 10 ml of water, cooled to 0° C. and added with 1.5 ml methanol under stirring. To this solution were added 4 ml dowex 1×4 anion exchange resin in the OH−-form and afterwards 192 μl of propionic anhydride and stirred for 2 hours at 4° C. Then the resin was removed by filtration and the solution was dialyzed contra water and freeze-dried.
 Hemocompatibility Measurements of Compounds According to the General Formula 1 by ISO 10933-4 (In Vitro Measurements):
 For the measurement of the hemocompatibility of the compounds according to formula 1 cellulose membranes, silicon tubes and stainless steel stents were covalently coated with a compound according to formula 1 and tested contra heparin as well as contra the corresponding, in the single tests utilised uncoated material surfaces.
 3.1. Cellulose Membranes (Cuprophan) Coated with Desulphated, Reacetylated Heparin (Ac-Heparin)
 For the examination of the coagulatory physiologic interactions between citrated whole blood and the Ac-heparin-resp. heparin-coated cuprophan membranes the open perfusion system of the Sakariassen-modified Baumgartner-chamber is used [Sakariassen K. S. et al.; J. Lab. Clin. Med. 102: 522-535 (1983)]. The chamber is composed of four building parts plus conical nipples and threaded joints and is manufactured of polymethylmethacrylate and allows the parallel investigation of two modified membranes, so that in every run a statistic coverage is included. The construction of this chamber permits quasi-laminar perfusion conditions.
 After 5 minutes of perfusion at 37° C. the membranes are extracted and after fixation of the adherent platelets the platelet occupancy is measured. The respective results are set into relation to the highly thrombogeneous subendothelial matrix as negative standard with a 100% platelet occupancy. The adhesion of the platelets takes place secondary before the formation of the plasma protein layer on the foreign material. The plasma protein fibrinogen acts as cofactor of the platelet aggregation. The such induced activation of the platelets results in the bonding of several coagulation associated plasma proteins, as e.g. vitronectin, fibronectin and von Willebrand-factor on the platelet surface. By their influence finally the irreversible aggregation of the platelets occurs.
 The platelet occupancy presents because of the described interactions an accepted quantum for the thrombogenity of surfaces in case of the foreign surface contact of blood. From this fact the consequence arises: the lower the platelet occupancy is on the perfunded surface the higher is the hemocompatibility of the examined surface to be judged.
 The results of the examined heparin-coated and Ac-heparin-coated membranes show clearly the improvement of the hemocompatibility of the foreign surface through the coating with Ac-heparin. Heparin-coated membranes show a 45-65% platelet occupancy, whilst Ac-heparin-coated surfaces show values from 0-5% (reference to subendothelial matrix with 100% platelet occupancy).
 The adhesion of the platelets on the Ac-heparinated surface is extremely aggravated due to the absent, for the activation of platelets essential plasma proteins. Unlike to this the heparin-coated surface with the immediately incipient plasma protein adsorption offers optimal preconditions for activation, deposition and aggregation of platelets, and ultimately the blood reacts with the corresponding defense mechanisms to the inserted foreign surface. Ac-heparin fulfills by far superior than heparin the requirements to the hemocompatibility of the foreign surface.
 The interaction of plasma protein adsorption and platelet occupancy as direct quantum for the thrombogenity of a surface, in dependence of the to the blood offered coating, is made clear especially well by this in-vitro test. Thus the utilisation of covalently bound heparin as antithrombotic operant surface is only strongly limited or not possible at all. The interactions of immobilised heparin with blood revert themselves here into the undesired opposite—the heparin-coated surface gets thrombogeneous.
 Obviously the outstanding importance of heparin as an antithrombotic is not transferable to covalently immobilised heparin. In the systemic application in dissolved form it can fully unfold its properties. But if heparin is not covalently immobilised, its antithrombotic properties, if at all, is only short-lived. Different is the Ac-heparin (“No-affinity”-heparin), that due to the desulphation and N-reacetylation in fact totally loses the active antithrombotic properties of the initial molecule, but acquires in return distinctive athrombogeneous properties, that are demonstrably founded in the passivity versus antithrombin III and the missing affinity towards coagulation initiating processes and remain after covalent bonding.
 Thereby Ac-heparin and thus the compounds of the general formula 1 in total are optimally suitable for the camouflage of foreign surfaces in contact with the coagulation system.
 3.2. Immobilisation on Silicone
 Through a 1 m long silicon tube with 3 mm inside diameter 100 ml of a mixture of ethanol/water 1/1 (v/v) was pumped in a circular motion for 30 minutes at 40° C. Then 2 ml 3-(triethoxysilyl)-propylamine were added and pumped in a circular motion for additional 15 hours at 40° C. Afterwards it was rinsed in each case for 2 hours with 100 ml ethanol/water and 100 ml water.
 3 mg of the deacetylated and reacetylated heparin (Ac-heparin) were dissolved at 4° C. in 30 ml 0.1 M MES-buffer pH 4.75 and mixed with 30 mg CME-CDI (N-cyclohexyl-N′-(2-morpholinoethyl)carbod iimidemethyl-p-toluenesulphonate). This solution was pumped in a circular motion for 15 hours at 4° C. through the tube. Afterwards it was rinsed with water, 4 M NaCl solution and water in each case for 2 hours.
 3.3 Determination of the Platelet Number (EN30993-4)
 In a 1 m long silicone tube with 3 mm inside diameter two 2 cm long formfitting glass tubes were placed. Then the tube was closed with a shrinkable tubing to a circle and filled under exclusion of air via syringes with a 0.154 M NaCl solution. In doing so one syringe was used to fill in the solution and the other syringe was used to remove the air. The solution was exchanged under exclusion of air (bleb-free) with the two syringes against citrated whole blood of a healthy test person. Then the recess holes of the syringes were closed by pushing the glass tubes over them and the tube was clamped taut into a dialysis pump. The blood was pumped for 10 minutes with a flow rate of 150 ml/min. The platelet content of the blood was measured before and after the perfusion with a coulter counter. For uncoated silicone tubes the platelet loss was of 10%. In contrast to it the loss was in silicon tubes, which were coated according to example 5.2, in average at 0% (number of experiments: n=3).
 Also in this dynamic test system it is shown, that the activation of platelets on an Ac-heparin coated surface is reduced. Simultaneously it can be recorded, that the immobilisation of heparin executes a negative effect on the hemocompatibility of the utilised surface. Against it Ac-heparin shows, in accordance to its passive nature, no effects in contact with the platelets.
 3.4 Whole Blood Experiments on 316 LVM Stainless Steel Coronary Stents
 In line with the biocompatibility experiments 31 mm long 316 LVM stainless steel stents were covalently coated with Ac-heparin. In case of a total surface of 2 cm2 and a occupancy coefficient of about 20 pm/cm2 stent surface the charging of such a stent is about 0.35 μg Ac-heparin. As comparison: in case of thrombosis prophylaxis the usual daily application rate of heparin is in contrast 20-30 mg and thus would correspond to the at least 60.000 times the value.
 These experiments were carried out with the established hemodynamic Chandler loop-system [A. Henseler, B. Oedekoven, C. Andersson, K. Mottaghy; KARDIOTECHNIK 3 (1999)]. Coated and uncoated stents were expanded and tested in PVC tubes (medical grade PVC) with 600 mm length and 4 mm inside diameter. The results of these experiments confirm the according to the silicone tubes discussed experiments. The initially to the stent attributed platelet loss in the perfusate of 50% is reduced by the refinement of the stent surface with Ac-heparin by more than 80%.
 The influence of in the tube expanded, surface modified coronary stents to the platelet loss is evaluated in further Chandler tests during a 45 minute whole blood perfusion. For this primarily the stent-free PVC tube is analysed, the outcome of this is the zero value. The empty tube shows an average platelet loss of 27.4% regarding to the donor blood at a standard aberration of solely 3.6%. This base value underlied different surface modified stents are expanded in the PVC tubes and are analysed under analogous conditions on the by them caused platelet loss. It occurs also in this case, that the stent covered surface, which solely accounts for about 0.84% of the total test surface, causes a significant and reproducable effect on the platelet content. According to the empty tube (base value) the analysis of the polished, chemically not surface coated stent yields an additional average platelet loss of 22.7%. Therewith causes this compared to the PVC empty tube less than 1% measurable foreign surface an approximately comparable platelet loss. A direct result is that the medicinal stainless steel 316 LVM used as stent material induces an about 100 times stronger platelet damage compared to a medical grade PVC surface, although this test surface only accounts for 0.84% of the total surface.
 The analysed surface coatings on the stainless steel coronary stents show to be able to reduce very clearly the enormous dimension of the stent induced platelet damage (see FIG. 2). As most effective proved with 81.5% the Ac-heparin (SH4).
 If the effects of the Ac-heparin-coated stents on the platelet loss are considered, then good congruent values result. The correlation of the platelet loss in the perfusate resp. the adhesion of the platelets to the offered surfaces show the reliability of the measurements.
 3.4.1 Covalent Hemocompatible Coating of Stents
 Not expanded stents of medicinal stainless steel LVM 316 were degreased in the ultrasonic bath for 15 minutes with acetone and ethanol and dried at 100° C. in the drying closet. Then they were dipped for 5 minutes into a 2% solution of 3-aminopropyltriethoxysilane in a mixture of ethanol/water (50/50: (v/v)) and then dried for 5 minutes at 100° C. Afterwards the stents were washed with demineralised water over night.
 3 mg desulphated and reacetylated heparin were dissolved at 4° C. in 30 ml 0.1 M MES-buffer (2-(N-morpholino)ethanesulphonic acid) pH 4.75 and mixed with 30 mg N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide-methyl-p-toluenesulphonate. In this solution 10 stents were stirred for 15 hours at 4° C. Then they were rinsed with water, 4 M NaCl solution and water in each case for 2 hours.
 3.4.2 Determination of the Glucosamine Content of the Coated Stents by HPLC
 Hydrolysis: the coated stents are given in small hydrolysis tubes and are abandoned with 3 ml 3 M HCl for exactly one minute at room temperature. The metal probes are removed and the tubes are incubated after sealing for 16 hours in the drying closet at 100° C. Then they are allowed to cool down, evaporated three times until dryness and taken up in 1 ml de-gased and filtered water and measured contra an also hydrolysated standard in the HPLC:
desulphat. + desulphat. + desulphat. + sample reacet. heparin area reacet. heparin reacet. heparin stent area [g/sample] [cm2] [g/cm2] [pmol/cm2] 1 129.021 2.70647E − 07 0.74 3.65739E − 07 41.92 2 125.615 2.63502E − 07 0.74 3.56084E − 07 40.82 3 98.244 1.93072E − 07 0.74 2.60908E − 07 29.91 4 105.455 2.07243E − 07 0.74 2.80058E − 07 32.10 5 119.061 2.33982E − 07 0.74 3.16192E − 07 36.24 6 129.202 2.53911E − 07 0.74 3.43124E − 07 39.33 7 125.766 2.53957E − 07 0.74 3.43185E − 07 39.34
 In Vivo Examination of Coated Coronary Stents (FIG. 5)
 4.1. In Vivo Examinations of Coronary Stents Coated with Ac-Heparin
 Due to the data on hemocompatibility, which Ac-heparin yielded in the in-vitro experiments, the suitability of the Ac-heparin surface as athrombogeneous coating of metal stents was discussed in vivo (animal experiment).
 The target of the experiments was primarily to evaluate the influence of the Ac-heparin coating on the stent induced vessel reaction. Besides the registration of possible thrombotic events the relevant parameters for restenotic processes like neointima area, vessel lumen and stenosis degree were recorded. For the examinations 6-9 month old domestic porks were used, one for the validation of stents for a long time established and approved animal model.
 As expected in these experiments neither acute, subacute nor late acute thrombotic events were registered, what may be assessed as proof for the athrombogeneous properties of Ac-heparin.
 After four weeks the animals were dispatched (euthanized), the stented coronary artery segments extracted and histomorphometrically analysed. Indications to a possible acute or subchronic toxicity, allergisation reactions or ulterior irritations as consequence of the implantation of Ac-heparin coated stents are not observed during the complete experimental phase, especially in the histologic examination. During the stent implantation as well as the follow-up coronary-angiographic data sets were ascertained, which permit an interpretation with regard to the vessel reaction to the stent implantation.
 The difference between the uncoated control stent and the Ac-heparin coated stent is unambiguous. The generation of a distinct neointima layer is in case of the uncoated control stent very well observable. Already after four weeks the proliferation promotional effect of the uncoated stent surface on the surrounding tissue occurs in such a degree, that ultimately the danger of the vessel occlusion in the stent area is given.
 Contrary in case of the Ac-heparin coated stents a clearly thinner neointima layer is observed, which argues for a well modulated ingrowth of the stent under maintenance of a wide, free vessel lumen.
 The detailed histomorphometric and coronary angiographic data substantiate this conclusion, as it can be observed congruently, that via the Ac-heparin coating (SH4) the neointima hyperplasia (“restenosis”) was repressed by about 17-20% in comparison to the uncoated control stent. This result is unexpected and remarkably at the same time. Surely it is not demanded of an athrombogeneous surface to have an influence also on processes that lead to a neointima hyperplasia, i.e. to prevent restenoses, in addition to the preposition of hemocompatible characteristics.
 On the one hand with a dense, permanent occupancy of the stent surface with Ac-heparin a direct cell contact to the metal surface is prevented. As in technical literature the emission of certain metal ions into the implant close tissue is discussed as one probable reason of restenosis, an anti-restenoic potency could be founded by one of the coating caused prevention of a direct metal contact.
 On the other hand such a positive side effect is plausible, because on a passive, athrombogenenous stent surface with the absence of a platelet aggregation also the proliferative effects of the thereby released growth factors are to be missed. Thus an important, starting from the lumen side, stimulus of the neointimal proliferation is omitted.
 Coating of the Stents with Taxol by the Spraying Method
 The via example 1 and example 2 prepared not expanded stents are balanced and horizontally hung onto a thin metal bar (d=0.2 mm), which is stuck on the rotation axis of the rotation and feed equipment and rotates with 28 r/min. The stents are fixed in that way, that the inside of the stents does not touch the bar. At a feeding amplitude of 2.2 cm and a feeding velocity of 4 cm/s and a distance of 6 cm between stent and spray valve the stent is sprayed with the particular spray solution. After the drying (about 15 minutes) at room temperature and proximate in the fume hood over night it is balanced again.
 Fabrication of the spray solution: 44 mg taxol are dissolved in 6 g chloroform.
stent no. before coating after coating coating mass 1 0.0194 g 0.0197 g 0.30 m
 Determination of the Elution Behaviour in PBS-Buffer
 Per stent in a sufficient small flask 2 ml PBS-buffer is added, sealed with para-film and incubated in the drying closet at 37° C. After expiry of the chosen time intervals in each case the excess solution is depipetted and its UV absorption at 306 nm is measured.
|Patente citada||Fecha de presentación||Fecha de publicación||Solicitante||Título|
|US4152513 *||1 Jun 1977||1 May 1979||University Of Delaware||Preparation of alkyl glycosides of amino sugars|
|US4430265 *||30 Jun 1982||7 Feb 1984||Takeda Chemical Industries, Ltd.||Glucosamine derivatives|
|US4572901 *||23 Jun 1983||25 Feb 1986||Children's Hospital Medical Center Of Northern California||Method and composition for protein immobilization|
|US4661345 *||26 Feb 1985||28 Abr 1987||The Rockefeller University||Method for treating pertussis|
|US5155110 *||26 Sep 1988||13 Oct 1992||Warner-Lambert Company||Fenamic acid hydroxamate derivatives having cyclooxygenase and 5-lipoxygenase inhibition|
|US5510077 *||15 Sep 1994||23 Abr 1996||Dinh; Thomas Q.||Method of making an intraluminal stent|
|US5922692 *||11 Mar 1998||13 Jul 1999||Marino; Richard P.||Concentration of glycosaminoglycans and precursors thereto in food products|
|US6090292 *||12 Feb 1998||18 Jul 2000||Fresenius Ag||Device for purifying solutions containing proteins and use of the device|
|US6179817 *||28 Ene 1999||30 Ene 2001||Boston Scientific Corporation||Hybrid coating for medical devices|
|US6238795 *||14 Ene 2000||29 May 2001||The Dow Chemical Company||Surface modified polymer beads|
|US6258121 *||2 Jul 1999||10 Jul 2001||Scimed Life Systems, Inc.||Stent coating|
|US6306166 *||14 Oct 1998||23 Oct 2001||Scimed Life Systems, Inc.||Loading and release of water-insoluble drugs|
|US6379379 *||13 Ago 1999||30 Abr 2002||Scimed Life Systems, Inc.||Stent with smooth ends|
|US6503538 *||30 Ago 2000||7 Ene 2003||Cornell Research Foundation, Inc.||Elastomeric functional biodegradable copolyester amides and copolyester urethanes|
|US7001894 *||18 Jul 2001||21 Feb 2006||Dainichiseika Color & Chemicals Mfg. Co., Ltd.||Blood flow improvers and thrombosis preventives or remedies|
|US20010000802 *||20 Dic 2000||3 May 2001||Medtronic, Inc.||Implantable system with drug-eluting cells for on-demand local drug delivery|
|US20010032014 *||18 Jun 2001||18 Oct 2001||Scimed Life Sciences, Inc.||Stent coating|
|US20010044654 *||27 Abr 2001||22 Nov 2001||Changyi Chen||Decellularized vascular prostheses resistant to thrombus occlusion and immunologic rejection|
|US20020032173 *||18 Jul 2001||14 Mar 2002||Koyo Chemical Co., Ltd.||Blood flow improvers and thrombosis preventives or remedies|
|US20020087123 *||2 Ene 2001||4 Jul 2002||Hossainy Syed F.A.||Adhesion of heparin-containing coatings to blood-contacting surfaces of medical devices|
|US20050060028 *||15 Oct 2002||17 Mar 2005||Roland Horres||Coating of stents for preventing restenosis|
|US20050176678 *||15 Abr 2003||11 Ago 2005||Roland Horres||Compounds and method for coating surfaces in a haemocompatibe manner|
|US20060172968 *||22 Dic 2005||3 Ago 2006||Benito Casu||Derivatives of partially desulphated glycosaminoglycans as heparanase inhibitors, endowed with antiangiogenic activity and devoid of anticoagulating effect|
|WO1999027976A1 *||27 Nov 1998||10 Jun 1999||Baxter Biotech Tech Sarl||Non thrombogenic polymers with compatibility with organic fluids and tissues|
|1||*||Brasen et al. "Angiogenesis, Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor-BB Expression, Iron Deposition, and Oxidation-Specific Epitopes in Stented Human Coronary Arteries" Arterioscler Thromb Vasc Biol., 2001, 21, 1720-1726|
|2||*||Lazardous, Daisy F., COmparative Effects of Basic Fibroblast Growth Factor and Vasuclar Endothelial Growth Factor on Coronary Collateral Development and the Arterial Response to Injury, Cirulcation (1996); 94, pgs. 1074-1082|
|3||*||Medinol product sheet (http://www.medinol.com/products_nirflex.asp), accessed 30 May, 2013|
|4||*||Nelson et al. "Endovascular Stents and Stent-Grafts: Is Heparin Coating Desirable?" Cardiovasc Intervent Radiol, 2000, 23, 252-255|
|5||*||Van Oeveren, W.; Schoen, P.; Maijers, C.A.; Monnink, S.H.; Van Boven, A.J. Progress in Biomedical Research "Hemocompatibility of Stents" 1999, 4 (1), 17-22|
|6||*||Wohrle et al. "Comparison of the heparin coated vs the uncoated Jostent® - no influence on restenosis or clinical outcome" European Heart Journal, 2001, 22, 1808-1816|
|Patente citante||Fecha de presentación||Fecha de publicación||Solicitante||Título|
|US7670282||14 Jun 2005||2 Mar 2010||Pneumrx, Inc.||Lung access device|
|US7678767||8 Dic 2004||16 Mar 2010||Pneumrx, Inc.||Glue compositions for lung volume reduction|
|US7761130||27 Mar 2007||20 Jul 2010||Dexcom, Inc.||Dual electrode system for a continuous analyte sensor|
|US7766891||8 Jul 2005||3 Ago 2010||Pneumrx, Inc.||Lung device with sealing features|
|US7766938||8 Jul 2005||3 Ago 2010||Pneumrx, Inc.||Pleural effusion treatment device, method and material|
|US7775968||14 Jun 2005||17 Ago 2010||Pneumrx, Inc.||Guided access to lung tissues|
|US7792562||22 Dic 2009||7 Sep 2010||Dexcom, Inc.||Device and method for determining analyte levels|
|US7828728||14 Feb 2007||9 Nov 2010||Dexcom, Inc.||Analyte sensor|
|US7835777||22 Dic 2009||16 Nov 2010||Dexcom, Inc.||Device and method for determining analyte levels|
|US7885697||10 Mar 2005||8 Feb 2011||Dexcom, Inc.||Transcutaneous analyte sensor|
|US7932225||23 Dic 2008||26 Abr 2011||Pneumrx, Inc.||Glue composition for lung volume reduction|
|US8050731||16 Nov 2005||1 Nov 2011||Dexcom, Inc.||Techniques to improve polyurethane membranes for implantable glucose sensors|
|US8053018||15 Ene 2010||8 Nov 2011||Dexcom, Inc.||Techniques to improve polyurethane membranes for implantable glucose sensors|
|US8070798||20 Jul 2007||6 Dic 2011||Josiah Wilcox||Drug eluting medical device and method|
|US8309520||23 Nov 2010||13 Nov 2012||Ls Medcap Gmbh||Fully synthetic albumin analog|
|US8343212||15 May 2008||1 Ene 2013||Biotectix, LLC||Polymer coatings on medical devices|
|US8501212||11 Mar 2011||6 Ago 2013||Carmeda Ab||Immobilised biological entities|
|US8523937||19 Nov 2008||3 Sep 2013||Cook Medical Technologies Llc||Controlled drug delivery using a zein layer modified with levulinic acid|
|US8632605||11 Sep 2009||21 Ene 2014||Pneumrx, Inc.||Elongated lung volume reduction devices, methods, and systems|
|US8682408||5 Mar 2010||25 Mar 2014||Dexcom, Inc.||Polymer membranes for continuous analyte sensors|
|US8865249||28 Sep 2012||21 Oct 2014||Dexcom, Inc.||Techniques to improve polyurethane membranes for implantable glucose sensors|
|US8877062 *||12 Jul 2010||4 Nov 2014||Interface Biologics, Inc.||Antithrombogenic hollow fiber membranes and filters|
|US8992963||15 Sep 2009||31 Mar 2015||Carmeda Ab||Immobilised biological entities|
|US9056152||23 Sep 2011||16 Jun 2015||Boston Scientific Scimed, Inc.||Medical device with crystalline drug coating|
|US20050281739 *||8 Dic 2004||22 Dic 2005||Glen Gong||Imaging damaged lung tissue using compositions|
|US20050281740 *||8 Dic 2004||22 Dic 2005||Glen Gong||Imaging damaged lung tissue|
|US20050281797 *||8 Dic 2004||22 Dic 2005||Glen Gong||Lung volume reduction using glue compositions|
|US20050281798 *||8 Dic 2004||22 Dic 2005||Glen Gong||Targeting sites of damaged lung tissue using composition|
|US20050281799 *||8 Dic 2004||22 Dic 2005||Glen Gong||Targeting damaged lung tissue using compositions|
|US20050281800 *||8 Dic 2004||22 Dic 2005||Glen Gong||Targeting sites of damaged lung tissue|
|US20110009799 *||13 Ene 2011||Interface Biologics, Inc.||Antithrombogenic hollow fiber membranes and filters|
|WO2011143284A1 *||11 May 2011||17 Nov 2011||Boston Scientific Scimed, Inc.||Endoprosthesis|
|WO2012028310A2 *||31 Ago 2011||8 Mar 2012||Avidal Vascular Gmbh||Pharmaceutical compositions comprising a taxane|
|Clasificación de EE.UU.||424/426, 623/1.42, 623/1.46|
|Clasificación internacional||A61K31/436, A61K31/728, C09D105/08, A61L31/10, A61L33/08, C08B37/00, C08B37/08, A61P7/02, A61L27/00, A61P19/06, A61P37/06, A61P3/06, A61K31/337, A61K31/165, A61L33/00, A61K31/366, A61L31/00, A61P35/00, A61L31/16, A61K31/727, A61L33/10, A61K45/00, C08B37/10|
|Clasificación cooperativa||A61K31/13, A61K31/21, A61K31/28, A61L31/16, A61K31/33, A61K31/045, C08L5/10, C08B37/0075, A61K31/095, A61K31/11, C09D105/08, A61L31/10, A61K31/727, A61L33/08, A61K31/722, A61K31/12, A61K31/075|
|Clasificación europea||A61L33/08, A61L31/10, C09D105/08, A61L31/16, A61K31/727, C08B37/00P2G|
|24 May 2004||AS||Assignment|
Owner name: HEMOTEQ GMBH, GERMANY
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:HORRES, ROLAND;LINSSEN, MARITA KATARINA;HOFFMAN, MICHAEL;AND OTHERS;REEL/FRAME:015531/0263;SIGNING DATES FROM 20040212 TO 20040511
|19 Ene 2005||AS||Assignment|
Owner name: HEMOTEQ GMBH, GERMANY
Free format text: CORRECTIVE ASSIGNMENT TO CORRECT THE DOCUMENT DATES SPELLING OF INVENTOR NAMES TITLE OF INVENTION PREVIOUSLY RECORDED ON REEL 015531 FRAME 0263;ASSIGNORS:HORRES, ROLAND;LINSSEN, MARITA KATARINA;HOFFMANN, MICHAEL;AND OTHERS;REEL/FRAME:015585/0397;SIGNING DATES FROM 20040212 TO 20040511
|29 Jul 2010||AS||Assignment|
Owner name: HEMOTEQ AG, GERMANY
Free format text: CHANGE OF NAME;ASSIGNORS:HORRES, ROLAND;LINSSEN, MARITA KATARINA;HOFFMAN, MICHAEL;AND OTHERS;SIGNING DATES FROM 20040212 TO 20040511;REEL/FRAME:024761/0867