US20090143464A1 - Method for preventing and/or treating peripheral Neuropathies induced by the administration of an anticancer agent - Google Patents

Method for preventing and/or treating peripheral Neuropathies induced by the administration of an anticancer agent Download PDF

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US20090143464A1
US20090143464A1 US12/320,430 US32043009A US2009143464A1 US 20090143464 A1 US20090143464 A1 US 20090143464A1 US 32043009 A US32043009 A US 32043009A US 2009143464 A1 US2009143464 A1 US 2009143464A1
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carnitine
acetyl
taxol
oxaliplatin
alc
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Claudio Cavazza
Claudio Pisano
Loredana Vesci
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Sigma Tau Industrie Farmaceutiche Riunite SpA
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Sigma Tau Industrie Farmaceutiche Riunite SpA
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Priority claimed from IT98RM000511 external-priority patent/IT1302121B1/en
Priority claimed from IT1999RM000206 external-priority patent/IT1306124B1/en
Priority claimed from US10/292,823 external-priority patent/US20030199535A1/en
Application filed by Sigma Tau Industrie Farmaceutiche Riunite SpA filed Critical Sigma Tau Industrie Farmaceutiche Riunite SpA
Priority to US12/320,430 priority Critical patent/US20090143464A1/en
Publication of US20090143464A1 publication Critical patent/US20090143464A1/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/205Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/21Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/22Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
    • A61K31/221Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin with compounds having an amino group, e.g. acetylcholine, acetylcarnitine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/28Compounds containing heavy metals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/335Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47Quinolines; Isoquinolines
    • A61K31/475Quinolines; Isoquinolines having an indole ring, e.g. yohimbine, reserpine, strychnine, vinblastine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/555Heterocyclic compounds containing heavy metals, e.g. hemin, hematin, melarsoprol
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00Medicinal preparations containing inorganic active ingredients
    • A61K33/24Heavy metals; Compounds thereof
    • A61K33/243Platinum; Compounds thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/14Peptides containing saccharide radicals; Derivatives thereof, e.g. bleomycin, phleomycin, muramylpeptides or vancomycin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/02Drugs for disorders of the nervous system for peripheral neuropathies
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • A61P35/04Antineoplastic agents specific for metastasis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00General protective or antinoxious agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the invention described herein relates to the use of L-carnitine and alkanoyl L-carnitines in the preparation of medicaments useful in the treatment of tumours, particularly in combination with anticancer agents.
  • U.S. Pat. No. 4,713,370 describes the use of carnitine in combination with cytostatic agents such as daunomycin, N-acetyl-daunomycin and daunomycin oxime to reduce the cardiac toxicity of these compounds.
  • U.S. Pat. No. 4,267,163 describes the use of carnitine in combination with cytostatic agents such as adriamycin, adriamycin-14-octanoate, 4′-epi-adriamycin, adriamycin beta-anomer and 4′-epi-adriamycin gamma-anomer to reduce the cardiac toxicity of these compounds.
  • peripheral neuropathies are defined as a group of persistent disturbances of the motor neurons of the brain stem and spinal cord and/or the primary sensory neurons and/or the peripheral autonomic neurons, with involvement of the peripheral axons and their attendant supporting structures.
  • peripheral neuropathies constitute a heterogeneous class of diseases because their etiology may be secondary to viral infections (herpes zoster), ischaemia (arteriosclerosis), metabolic unbalances (diabetes, renal and liver insufficiency), drug-induced toxicity (adriamicine, isoniazide, nitrofurantoin), mechanical stresses (compression, entrapment, fracture or dislocation of bones), radiations, genetic factors and pathologies of the immune system.
  • viral infections herepes zoster
  • ischaemia arteriosclerosis
  • metabolic unbalances diabetes, renal and liver insufficiency
  • drug-induced toxicity adriamicine, isoniazide, nitrofurantoin
  • mechanical stresses compression, entrapment, fracture or dislocation of bones
  • radiations genetic factors and pathologies of the immune system.
  • Acetyl L-carnitine by increasing the levels of reduced glutathione, one of the most important antioxidant metabolites, and probably the levels of the cytochrome oxidases, might bring about the free radicals scavenge and the restoration of the tetravalent oxido-reductant respiratory mechanism in general and of the neurons in particular. Moreover, acetyl L-carnitine would affect the neurons by bringing about an increase in ATP-ase and AChE that are indispensable for optimum neurotransmission.
  • Carboplatin is a structural analogue of Cisplatin and its associated nephrotoxicity, though by no means negligible, is less than that of Cisplatin.
  • Peripheral neurotoxicity is the dose-limiting side effect of the antineoplastic drug Cisplatin (Cavaletti et al. Journal of the Peripheral Nervous System , Vol. 6, Number 3; September 2001, 136-2001 Meeting of the Peripheral Nerve Society).
  • Vincristine is a well-known anticancer agent which has toxic effects, particularly at the level of the immune system.
  • Taxol is a natural extract, first isolated from the bark of Taxus brevifolia , with anticancer properties and has proved neurotoxic and myelotoxic in human subjects. It is used for the treatment of tumours resistant to platinum therapy, but gives rise to greater cumulative toxicity in the peripheral nervous system. It has also been ascertained that Taxol induces neutropenia in the subjects treated (Rowinsky et al.; Semin. Oncol . (1993), Aug. 20 (4 suppl 3), 1-15; Onetto et al. J. Natl. Cancer Inst. Monogr . (1993); (15):131-9).
  • Bleomycin is typically used in young patients with testicular cancer, lymphoma, and other types of tumours.
  • the pulmonary toxicity of Bleomycin is characterised by destruction of the alveolar epithelial barrier and the consequent intra-alveolar proliferation of fibroblasts and deposition of extracellular matrix components (Karam H et al.; Cell Biol. Toxicol (1998 February); 14(1):13-22).
  • Type 2 pneumocytes are not capable of regenerating the damaged or lost epithelium.
  • chemotherapeutic drugs induce peripheral neurotoxicity: the taxoids, the platinum compounds, the vinca alkaloids and the epothilone class.
  • Severe peripheral neuropathy can induce therapy modification, while mild or moderate cases still represent an important tolerability problem limiting patient quality of life.
  • the vinca alkaloids bind to tubulin and prevent the polymerisation from soluble dimers into microtubules
  • the taxoids promote the formation of microtubules and prevent their depolymerisation, which results in an abundance of rigid microtubules.
  • Microtubules are essential components for the maintenance of cell shape, a variety of cellular actions and axoplasmic transport. Defective function of microtubules in neurons and axons may be the origin of the neurotoxicity of both families of chemotherapeutic agents.
  • Taxoids induce primarily a symmetrically distributed sensory distal neuropathy, which is related to both single and cumulative doses of the drug and is possibly dependent on the regimen.
  • the neurotoxicity of Taxol is due to a unique mechanism of action; it binds to tubulin and promotes microtubule assembly (Schiff P. B. et al., Nature 1979; Parnass J. and Horowitz S. B., J. Cell. Biol. 1981).
  • Microtubules formed in the presence of Taxol are stable in conditions which ordinarily depolymerize them, including the presence of calcium and cold.
  • Paclitaxel also alters the kinetics of microtubule assembly, eliminating the 3- to 4-minute lag time normally observed prior the initiation of assembly (Horowitz S. B. et al., Ann. N.Y. Acad. Sci. 1986). This mechanism contrasts with that of the vinca alkaloids which inhibit microtubule assembly.
  • neurotoxicity With the use of hemopoietic growth factor rescue of myelotoxicity, neurotoxicity becomes the dose-limiting factor (Schiller et al., J. Clin. Oncol. 1994). Symptoms may begin as early as 24 to 72 hours after treatment with high single doses (>250 mg/m 2 ), but the neurotoxicity is typically cumulative, with symptoms progressing after each treatment at both high and low doses.
  • the symptoms are generally tolerable (NCI grade 1 or 2).
  • the neurologic examination characteristically reveals an elevated threshold and perception of vibration in a distal, symmetric, glove-and-stocking pattern.
  • large-fiber modalities vibration, proprioception
  • loss of small-fiber modalities pain, temperature
  • Deep tendon reflexes are also frequently affected, with the distal (ankle) reflex being invariably absent or reduced.
  • Nerve conduction studies show reductions of sensory nerve action potential amplitudes in a symmetric, distal, length-dependent fashion. Sural sensory nerve action amplitude is virtually always reduced or absent in symptomatic patients.
  • Cisplatin induces a peripheral sensory axonal neuropathy, affecting large-diameter and, to a lesser extent, small-diameter sensory fibres. Paraesthesias and impaired propiocepsis are the most common symptoms (Thompson S. W. et al., Cancer 1984). Cisplatin accumulates in and damages the dorsal root ganglia, axonal changes being secondary to neuronal damage (Warner E., Int. J. Gynecol. Cancer 1996).
  • Cisplatin-induced neuropathy depends largely on the cumulative dose, the onset of symptoms being seen at a total dose of Cisplatin 330 to 400 mg/m 2 .
  • Cisplatin binds tightly and irreversibly to nerve tissue, which explains the deterioration of neurological condition which sometimes occurs after cessation of Cisplatin therapy.
  • the vinca alkaloids induce a peripheral sensory-motor polyneuropathy and autonomic neuropathy, which appears to be partially reversible after some months (Potsma T. J. et al., J. Neurooncol. 1993).
  • the epothilone family has a toxicological profile similar to the taxanes one (Lee F Y et al Proc Am Assoc Cancer Res, 2002, 792).
  • One of the general problems of pharmacological therapy is the therapeutic index of the agents, that is to say the ratio of the therapeutically effective dose to the toxic dose, or, at any rate, the dose that gives rise to the onset of side effects.
  • a peripheral neuropathy-inducing anticancer agent in particular of the taxane, platin, epothilone and vinca alkaloid families and Bleomycin, in particular Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine, and Vincristine, and a detoxifying amount of L-carnitine or of an alkanoyl L-carnitine, in which the linear or branched alkanoyl has 2-8 carbon atoms, or one of its pharmacologically acceptable salts, affords a potent protective effect against the toxicity and side effects of the anticancer agent, without impairing its efficacy, thus giving rise, amongst other things, to a substantial improvement in the quality of life and a prolonging of life itself in the subjects treated, whether human subjects or animals.
  • R is hydrogen or an alkanoyl group with 2 to 8 carbon atoms
  • X ⁇ represents the anion of a pharmaceutically acceptable salt, for the preparation of a medicament comprising an anticancer agent, characterised in that said compound produces a synergistic effect on the activity of the anticancer agent.
  • an object of the invention described herein is the co-ordinated use of the compound of formula (I) according to which the side effects of the anticancer agent in said medicament are substantially reduced.
  • a further object of the invention described herein is the use the compound of formula (I) in the preparation of a medicament useful for inhibiting metastases.
  • Yet another object of the invention described herein are combinations of the compound of formula (I) with anticancer agents and the related pharmaceutical compositions.
  • the implementation of the invention described herein also contributes to improving the quality of life of the patients treated; one need only think of the physical suffering caused by peripheral neuropathy, neutropenia, respiratory complications or debilitation due to weight loss caused by these agents.
  • co-ordinated use of the aforesaid compounds is, indifferently, either (i) co-administration, i.e. the substantially simultaneous or sequential administration of L-carnitine or an alkanoyl L-carnitine or one of its pharmacologically acceptable salts and of an anticancer agent, or (ii) the administration of a composition comprising the aforesaid active ingredients in combination and in a mixture, in addition to optional pharmaceutically acceptable excipients and/or vehicles.
  • the invention described herein thus covers both the co-administration of L-carnitine or an alkanoyl L-carnitine or one of its pharmacologically acceptable salts of formula (I) and of the anticancer agent, and pharmaceutical compositions, which can be administered orally, parenterally or nasally, including controlled-release forms, comprising the two active ingredients in a mixture.
  • the alkanoyl L-carnitine is selected from the group consisting of acetyl L-carnitine (hereinafter abbreviated to ALC or Alcar), propionyl L-carnitine (hereinafter abbreviated to PLC), butyryl L-carnitine, valeryl L-carnitine and isovaleryl L-carnitine, or one of their pharmacologically acceptable salts.
  • acetyl L-carnitine hereinafter abbreviated to ALC or Alcar
  • propionyl L-carnitine hereinafter abbreviated to PLC
  • butyryl L-carnitine valeryl L-carnitine and isovaleryl L-carnitine
  • valeryl L-carnitine isovaleryl L-carnitine
  • acetyl L-carnitine acetyl L-carnitine
  • an anticancer agent such as Taxol, acetyl L-carnitine and propionyl L-carnitine, or of Bleomycin and acetyl L-carnitine, or—a further possibility—of acetyl L-carnitine and Taxol or Cisplatin or Carboplatin or Oxaliplatin or Epothilone or Vincristine or Vinorelbine.
  • an anticancer agent such as Taxol, acetyl L-carnitine and propionyl L-carnitine, or of Bleomycin and acetyl L-carnitine, or—a further possibility—of acetyl L-carnitine and Taxol or Cisplatin or Carboplatin or Oxaliplatin or Epothilone or Vincristine or Vinorelbine.
  • the most preferred acetyl L-carnitine can be used in the co-ordinated use.
  • the intended use of the co-administration in the context of the present invention means the possibility to perform a preventive treatment (intended also as prevention) for the possible adverse effect of the anticancer agent.
  • the present invention means the therapeutic treatment (intended also as treatment or therapy) of the adverse effect of the anticancer agent.
  • the alkanoyl L-carnitine preferably acetyl L-carnitine
  • the alkanoyl L-carnitine shall be administered in view of the need of a treatment with an anticancer agent, known to imply adverse effects, in particular peripheral neuropathy.
  • the start of the administration, the subsequent schedule (namely, the posology) and the decision upon continuing the administration even after the start of the treatment with the anticancer agent shall be within the knowledge of the clinical expert, depending also on the kind of anticancer agent, the type and severity of the foreseen adverse effect, the conditions of the patient.
  • the administration of the alkanoyl L-carnitine, preferably acetyl L-carnitine can be started immediately before or immediately after surgical removal of the tumor, but in any case before the start of the administration of the anticancer agent, also in the case the agent be administered before surgery.
  • both prevention and treatment may apply, whenever the case.
  • Co-administration also means a package, or manufactured article, comprising distinct administration forms of L-carnitine or one of the aforesaid alkanoyl L-carnitines, or one of their pharmacologically acceptable salts and of an anticancer agent, accompanied by instructions for the co-ordinated simultaneous or time-scheduled intake of the active ingredients according to a dosage regimen established by the primary care physician, on the basis of the patient's condition.
  • This package is suitable both for prevention and treatment.
  • the package or manufactured article comprises a unit dosage containing both the anticancer agent and the alkanoyl L-carnitine, preferably acetyl L-carnitine.
  • a pharmacologically acceptable salt of L-carnitine or of an alkanoyl L-carnitine is any salt of the latter with an acid that does not give rise to toxic or side effects.
  • Examples of pharmacologically acceptable salts of L-carnitine or of the alkanoyl L-carnitines are chloride; bromide; iodide; aspartate; acid aspartate; citrate; acid citrate; tartrate; acid tartrate; phosphate; acid phosphate; fumarate; acid fumarate; glycerophosphate; glucose phosphate; lactate; maleate; acid maleate; mucate; orotate, oxalate; acid oxalate; sulphate; acid sulphate; trichloroacetate; trifluoroacetate; methane sulphonate; pamoate and acid pamoate.
  • One preferred form of daily dosing of L-carnitine or alkanoyl L-carnitine for clinical use is a composition comprising an amount of L-carnitine or an alkanoyl L-carnitine, preferably acetyl or propionyl L-carnitine, equivalent to 0.1 to 3 g, and preferably 0.5 to 3 g.
  • the invention described herein is advantageous in the prevention or treatment of toxic or side effects such as weight loss, heart, kidney and central nervous system damage, peripheral nervous system damage, peripheral neuropathy and particularly the myelosuppression and lung damage caused by the above-mentioned anticancer agents.
  • substantially protective effect is the prevention, reduction or elimination or anyway treatment of the side effect to a statistically significant extent.
  • the embodiment of the invention described herein also contributes to healing and to prolonging the lives of the patients thanks to the increase in therapeutic success due to the possibility of maintaining the scheduled treatment protocols or of increasing the doses of the chemotherapeutic agent, without having to discontinue the treatment due to contraindications.
  • a further benefit which is obtained with the invention described herein is related to the quality of life of the subjects treated; in fact, as already mentioned, the elimination or reduction of the physical suffering caused by a peripheral neuropathy or by debilitation due to weight loss favours the patient's ability to be self-sufficient. From the economic standpoint, there are obvious savings in terms of the costs borne by hospital facilities or by the families for the patient's care.
  • Myelosuppression is one of the toxic side effects that may manifest themselves as a result of administration of Taxol, a chemotherapeutic agent used in the therapy of various tumours, for example, of the breast, ovaries, lungs (small cell and otherwise), head and neck (Slichenmeyer and Von Hoff; J. Clin. Pharmacol . (1990), 30, 770-778).
  • Taxol a chemotherapeutic agent used in the therapy of various tumours, for example, of the breast, ovaries, lungs (small cell and otherwise), head and neck (Slichenmeyer and Von Hoff; J. Clin. Pharmacol . (1990), 30, 770-778).
  • the vehicle adopted for Taxol also in the commercial pharmaceutical forms (TAXOL®, Bristol Myers Squibb), is a derivative of polyethoxylated castor oil, known commercially as Cremophor® EL and is capable of inducing histamine release and anaphylactoid reactions in the dog and in human subjects (Slichenmeyer and Von Hoff; ibid; Bury et al.; Allergy (1992), 47, 624-629; Hershkoviz et al.; J. Leukoc. Biol . (1994), 56, 495-501; Inokuma et al.; J. Vet. Med. Sci . (1994), 56, 45-49).
  • Cremophor® EL polyethoxylated castor oil
  • L-carnitine combined with an anticancer agent such as Taxol, Carboplatin or Vincristine, ensures an extension of survival of the subject treated.
  • acetyl L-carnitine has shown an unexpected degree of protective activity against side effects induced by one of the anticancer agents Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine, much particularly peripheral neuropathy, but also myelosuppression and weight loss.
  • This protective effect shall be understood both as prevention of said side effect and as treatment of said protective effect.
  • NGF is extensively studied in the art as a neuroprotective agent in relation to Cisplatin and Taxol neurotoxicity.
  • the findings of the present invention indicate that acetyl L-carnitine is able to potentiate the effect of NGF on PC12 cells by enhancing expression of different genes, as assessed by microarray analysis, and a relationship between NGF and acetyl L-carnitine in promoting PC12 neuritis outgrowth was also reported.
  • Another definite relationship between acetyl L-carnitine and NGF was found even in the animal model of chronic Cisplatin-induced sensory neuropathy. In this model, a close relationship has already been previously demonstrated between the onset of a sensory neuropathy and the decrease in NGF circulating levels.
  • acetyl L-carnitine co-treatment significantly reduces the severity of Cisplatin-induced sensory neuropathy and, accordingly, the circulating levels of NGF are significantly higher in the acetyl L-carnitine co-treated subjects than in Cisplatin-treated ones.
  • acetyl L-carnitine is a specific protective agent for chemotherapy-induced neuropathy following treatment with the anti-cancer agents of interest in the present invention. Relevant to this point is the lack of any interference with the antitumor activity of the drugs.
  • acetyl L-carnitine can enhance the supportive effect of physiological NGF during chemotherapy-induced neuropathy, thus avoiding the problem of the local and general side effects of the exogenous administration of NGF which are a major problem of this neuroprotective strategy.
  • acetyl L-carnitine has shown surprising antimetastatic activity when administered concomitantly with Taxol, particularly in lung cancer.
  • propionyl L-carnitine has shown an unexpected synergistic effect in combination with Taxol.
  • Taxol induces severe neutropenia with a nadir after the 3rd-4th injection of the compound.
  • ALC can be conveniently administered orally, without, for that reason, excluding other administration routes which an expert in the field may deem it advisable to adopt, particularly, by injection, to be administered concomitantly, for example, in the same infusion vial, with the anticancer agent, or in sequence, as established by the expert in the field.
  • propionyl L-carnitine has shown a synergistic effect with the therapeutic activity of Taxol.
  • acetyl L-carnitine as a protective agent and one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine.
  • This combination can also comprise other anticancer agents which induce substantially reduced side effects, much particularly peripheral neuropathy, as a result of the combination according to the invention described herein. This latter embodiment is advantageous in case of polychemotherapy. It may also be advantageous to add L-carnitine to the above-mentioned combination.
  • acetyl L-carnitine as a protective agent
  • propionyl L-carnitine as a synergistic agent
  • one of the anticancer agents select selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine.
  • This combination also comprises other anticancer agents which show a synergistic effect or induce substantially reduced side effects as a result of the combination according to the invention described herein. This latter embodiment is advantageous in case of polychemotherapy. It may also be advantageous to add L-carnitine to the above-mentioned combination.
  • One specific object of the invention described herein is a pharmaceutical composition
  • a pharmaceutical composition comprising a therapeutically effective amount of one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine together with a protective amount of acetyl L-carnitine and a synergistic amount of propionyl L-carnitine, in a mixture with pharmaceutically acceptable vehicles and/or excipients.
  • kits containing a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine, as defined above, in an amount suitable for producing a synergistic effect with said anticancer agent; c) a pharmaceutical combination comprising at least one alkanoyl L-carnitine and/or L-carnitine, as defined above, in an amount suitable for producing a substantially protective action against the side effects of said anticancer agent.
  • kit according to the invention described herein may also be presented in the form of a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine in an amount suitable for producing a synergistic effect with said anticancer agent.
  • the kit according to the invention described herein may also be presented in the form of a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; and b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine and/or L-carnitine in an amount suitable for producing a substantially protective action against the side effects of said anticancer agent.
  • kits refer to one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine and Bleomycin as the anticancer agents.
  • Taxol or Cisplatin or Oxaliplatin or Epothilone or Vinorelbine as the anticancer agent
  • acetyl L-carnitine as the substantially protective agent
  • propionyl L-carnitine as the substantially synergistic agent
  • the purpose of this experiment is to demonstrate and evaluate the protective effect expressed as an increase in survival time, induced by L-carnitine in a murine experimental model.
  • Groups of 10 male Wistar rats aged 3 months were used, housed at 22 ⁇ 2° C., with 50 ⁇ 15% relative humidity and a 12 hour light/darkness cycle, maintained with water and feed “ad libitum”.
  • the rats were treated with the anticancer agents intravenously (i.v.) at the doses corresponding to their respective LD 30 , LD 50 and LD 80 .
  • the purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention, of acetyl L-carnitine administered one week prior to Taxol at two different doses of the latter (16 mg/kg and 8 mg/kg), by measuring the sensory nerve conduction velocity (SNCV), determined on the tail.
  • SNCV sensory nerve conduction velocity
  • mice Female Wistar rats aged 3 months (Charles River) were used, housed at 22 ⁇ 2° C., with 50 ⁇ 15% relative humidity and a 12 hour light/darkness cycle.
  • the rats were identified immediately prior to treatment by means of progressive numbers on their tails and were maintained with water and feed “ad libitum”.
  • the substances used were acetyl L-carnitine and Taxol.
  • the following treatment schedule was used: the Sham animals received Taxol solution solvent (cremophor/ethanol) intraperitoneally (i.p.); Taxol was administered i.p. once a week for 5 weeks; the treatments with acetyl L-carnitine, 200 mg/kg, were administered orally (os) once daily via a gastric tube, starting one week prior to the first Taxol administration and continuing for another 5 weeks (6 weeks in all).
  • Taxol solution solvent cremophor/ethanol
  • acetyl L-carnitine 200 mg/kg
  • the animals were anaesthetised with a gaseous mixture composed of 0.45 halothane, nitrogen protoxide and oxygen.
  • Nerve conduction velocity was measured in the tail, by placing stimulating electrodes at the base of the tail and a couple of recording ring electrodes distally in the tail 5 and 10 cm with respect to the stimulating electrodes.
  • the site of stimulations and recording were kept fixed on repeated examinations performed in each animal by marking the tail with permanent ink.
  • the recordings and stimulations of the tail nerve were done using an Ote Biomedica Phasis II electromyograph using a stimulation intensity equal to the threshold value with a duration of 100 microseconds.
  • a stimulation intensity equal to the threshold value with a duration of 100 microseconds.
  • the velocity was measured in all groups of animals both in basal conditions and after 5 weeks of treatment.
  • results were expressed as means ⁇ standard deviation; significance was assessed using the “t”-test, for both independent and paired data, with a statistical significance cut-off of p ⁇ 0.05.
  • the sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 2 here below.
  • Taxol-induced neuropathy sensory nerve conduction velocity (m/s) measured on the animals' tails in basal conditions and after treatment with acetyl L-carnitine.
  • Taxol solvent Treatment with the Taxol solvent (Sham group) did not modify nerve conduction velocity values as compared to the control group.
  • the administration of Taxol induced a significant reduction (P ⁇ 0.01) in sensory nerve conduction velocity compared to the control group; this reduction was dose-dependent: ⁇ 17% with the 16 mg/kg dose and ⁇ 9% with the 8 mg/kg dose.
  • acetyl L-carnitine is capable of affording statistically significant protection against Taxol-induced neurotoxicity.
  • the animals used in the preceding experimental model were weighed prior to starting treatment (basal values) and at the end of treatment.
  • Peripheral neuropathy was induced by two Taxol cycles (2.7 mg/kg/1.5 ml once a day for 5 days), 6 days apart.
  • Acetyl L-carnitine was administered subcutaneously, during the follow-up period (recovery). The dose of 100 mg/Kg/day was administered on the basis of individual body weight. Sham (NaCl 0.9%), Vehicle (Cremophor®-ethanol 1:1) and Taxol-treated animals were treated with the vehicle.
  • NCV Nerve conduction velocity
  • NCV was determined as in Example 2.
  • the sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 4 here below.
  • Taxol-induced neuropathy sensory nerve conduction velocity (m/s) measured on the animals' tails in basal conditions and after treatment with acetyl L-carnitine.
  • NGF plasma concentrations are given in the Table 5 here below.
  • PNS Peripheral Sensory Neuropathy
  • Taxol when injected according to the same schedule used for the evaluation of neutrophil granulocytes, was found to significantly inhibit the growth of L-MM3, which was monitored until the tumour reached approximately 2 cm in size. Combined treatment with Taxol and ALC for 14 days did not affect the anticancer action of Taxol.
  • Taxol caused severe neutropenia and ALC, administered continuously over the period in which this type of bone marrow toxicity occurs, was capable of preventing the Taxol-induced reduction in polymorphonucleates.
  • the action of ALC did not affect the anticancer activity of Taxol.
  • Taxol was found to induce a significant reduction in neutrophil granulocytes within only 6 hours of the third injection, and thus severe neutropenia occurs with a nadir after the 3rd-4th injection.
  • Acetyl L-carnitine inner salt (sterile ampoule), solubilised in water for injectable preparations, was used.
  • Each ampoule of ALC is dissolved in 4 ml of water for injections (Solution O).
  • Two ml of (O) are brought up to 25 ml with sterile PBS (Sigma), so as to have 10 mg/ml for subcutaneous administration (100 mg/kg/10 ml); 0.8 ml of (O) are brought to 50 ml with PBS in order to have 2 mg/ml for oral administration (100 mg/kg/50 ml).
  • Taxol (paclitaxel (INDENA)) is weighed, solubilised in the previously prepared specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C. sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • PBS phosphate buffer
  • mice 4-5 mice per cage; temperature 22 ⁇ 2° C.; relative humidity 55 ⁇ 15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7 ⁇ 20.7 ⁇ 14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • the randomisation is casual in blocks of animals, i.e. the animal house attendant transfers the mice from the boxes to the cages, completing one cage at a time.
  • the attendant provisionally identifies all the mice, weighs them, and, if the weights present significant differences between groups, moves the animals from one cage to another, keeping the number of animals per cage unchanged, so as to have well-matched overall weights between one cage and another.
  • Each cage is labelled with a card bearing the number of the group, the type of treatment (substance and/or substances injected, dose, administration route).
  • Each animal is identified with a number from 1 to 5, written on the tail in indelible ink.
  • mice are weighed prior to the start of treatment, and on day 5 or 7 and day 11.
  • the animals were treated from day 1 to day 10 with the molecules; Taxol or the vehicle were administered on alternate days, on days 5, 7, 9 and 11.
  • the groups are: 1) blanks; 2) vehicle+PBS; 3) Taxol; 4) Taxol+ALC; 5) ALC; 6) ALC+vehicle.
  • the animals are sacrificed 6 hours after the last Taxol injection.
  • Blood and bone marrow samples are taken 6 hours after the last treatment with Taxol or vehicle.
  • the mice are anaesthetised with CO 2 ; blood is taken from the retro-orbital plexus (0.5 ml blood/mouse) and placed in Eppendorf test tubes containing 10 ⁇ l of Vister heparin (5000 U/ml). The animals are sacrificed by cervical dislocation. Later, the bone marrow samples are taken.
  • One blood sample and one bone marrow sample per mouse are taken at various times.
  • the instrument Before starting the WBC (White Blood Cells) count, the instrument is checked by measuring the EMACHECK blood sample parameters (human check) supplied by Delcon.
  • the instrument is used according to the instructions provided in the operating manual.
  • the blood (25 ⁇ l) is taken from the dilutor and brought to a volume of 10 ml with isotonic solution (PLTA Saline, Delcon) in a beaker (dil. 1:400) (Solution A).
  • Solution A From Solution A, the dilutor takes 100 ⁇ l and brings them to 10 ml (dil. 1:100) in another beaker (Solution B).
  • To Solution A are added 3 drops of lysing agent (Emosol, Delcon), the solution is mixed by hand and left to act for approximately 2 minutes so that the red blood cells are lysed and HGB (haemoglobin) released.
  • Solution A containing the lysing agent is used for the WBC and haemoglobin (HGB) readings.
  • Solution B is used for the RBC and platelet (PLT) readings.
  • Superfrost plus slides 25 ⁇ 75 ⁇ 1 mm (Mensel-Glaser), ready for use, are adopted.
  • the blood (8 ⁇ l) is deposited on the right-hand side of the slide; another slide, placed at a 45° angle, to the left of the blood, is drawn back until in comes into contact with the drop which spreads rapidly along the contact line between the two slides; the slide is moved forwards with a smooth, rapid movement so as to obtain a thin film of blood.
  • the slide is left to dry in air, stained with Diff-Quick (DADE) dye, according to the attached instructions, and dried again in air.
  • DADE Diff-Quick
  • the slides are immersed in Histolemon solution (Carlo Erba) for 2 sec.; a drop of synthetic mountant (Shandon) is deposited at the centre of the slide and a cover slide is placed over it covering the entire blood smear, taking care not to form bubbles between the two slides.
  • Histolemon solution Carlo Erba
  • Shandon synthetic mountant
  • the slides are dried and then the WBC count is performed, up to 100, with an optical microscope, after depositing a drop of cedar oil on the slide.
  • the quantity of WBC/ml, assessed using the haemocytometer, is multiplied by the percentage value of the corresponding neutrophil granulocytes of the leukocyte formula. This parameter, divided by 100, expresses the value of neutrophils/mm 3 blood.
  • Taxol treatment (30 mg/kg i.p. every 2 days for a total of 4 times) caused significant neutropenia 6 hours after the last injection of the agent ( ⁇ 90% neutrophilic granulocytes compared to blanks, p ⁇ 0.001).
  • the oral or subcutaneous administration of acetyl L-carnitine (100 mg/kg) was found to protect the polymorphonucleates against Taxol-induced damage 8-43% sc; ⁇ 23% os) (Table 7).
  • Taxol administered 4 times on alternate days, induces severe neutropenia.
  • the oral administration of ALC is capable of affording significant protection against the damaging effect of Taxol.
  • example 7 was repeated, except for the Taxol administration route, which in this case was intravenous, that is to say in the actual clinical application conditions.
  • Acetyl L-carnitine inner salt (sterile ampoule), solubilised in water for injectable preparations, was used. Each ampoule of ALC is dissolved in 4 ml of water for injections (Solution O). 0.8 ml of (O) are brought to 50 ml with sterile PBS (Sigma) in order to perform the oral administration (100 mg/kg/50 ml).
  • Taxol (paclitaxel (INDENA)) is weighed, solubilised in the previously prepared specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C. sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • PBS phosphate buffer
  • mice 5 mice per cage; temperature 22 ⁇ 2° C.; relative humidity 55 ⁇ 15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7 ⁇ 20.7 ⁇ 14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • the randomisation is casual in blocks of animals, i.e. the animal house attendant transfers the mice from the boxes to the cages, completing one cage at a time.
  • the attendant provisionally identifies all the mice, weighs them, and, if the weights present significant differences between groups, moves the animals from one cage to another, keeping the number of animals per cage unchanged.
  • Each cage is labelled with a card bearing the number of the group, the type of treatment (substance and/or substances injected, dose, administration route).
  • Each animal is identified with a number from 1 to 5, written on the tail in indelible ink.
  • mice are weighed prior to the start of treatment, and on the day of the last Taxol injection.
  • Treatment schedule 1 the transplantable murine breast cancer cells (L-MM3) of Balb/c origin are grown at 37° C. in plastic flasks in a 5% CO 2 humidified incubator in DMEM medium containing 5% heat-inactivated FCS, L-glutamine 2 mM, and gentamicin 80 ⁇ g/ml. At the time of inoculation, the cells are detached with trypsin-EDTA and re-suspended in the same medium without PBS.
  • ALC is administered then for 10 days; the animals are sacrificed after 6 hours.
  • mice The experimental groups, each consisting of 15 mice, are:
  • Treatment schedule 2 the same experimental groups mentioned above, each consisting of 15 mice (with different cage numbers) are treated as follows in order to evaluate survival and tumour size.
  • ALC administration lasts 14 days (in groups 16, 17, 18) and the tumour is measured until it reaches a size of approximately 2 cm. The animals are left alive for 100 days.
  • mice The experimental groups, each consisting of 15 mice, are:
  • tumour size (length and width) is measured twice a week with a calliper, from the time the tumour is measurable.
  • Tumour size is expressed in cm and is evaluated according to the following formula: ⁇ (length ⁇ width).
  • Tumour measurement schedule Day after inoculation Cage (comprising 5 mice) 22 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 23 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 28 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 30 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 35 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 36 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 42 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 44 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 49 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24 49 13, 16, 19, 22 14, 17, 20, 23 15, 18, 21, 24
  • Blood and bone marrow samples are taken for the first treatment schedule animals. On the days they are sacrificed, the mice are anaesthetised with CO 2 ; blood is taken from the retro-orbital plexus (0.5 ml blood/mouse) and placed in Eppendorf test tubes containing 10 ⁇ l of Vister heparin (5000 U/ml). The animals are sacrificed by cervical dislocation. Later, the bone marrow samples are taken.
  • One blood sample and one bone marrow sample per mouse are taken at various times.
  • the instrument Before starting the WBC count, the instrument is checked by measuring the EMACHECK blood sample parameters (human check) supplied by Delcon.
  • the instrument is used according to the instructions provided in the operating manual.
  • the blood (25 ⁇ l) is taken from the dilutor and brought to a volume of 10 ml with isotonic solution (PLTA Saline, Delcon) in a beaker (dil. 1:400) (Solution A).
  • Solution A From Solution A, the dilutor takes 100 ⁇ l and brings them to 10 ml (dil. 1:100) in another beaker (Solution B).
  • To Solution A are added 3 drops of lysing agent (Emosol, Delcon), the solution is mixed by hand and left to act for approximately 2 minutes so that the red blood cells are lysed and HGB (haemoglobin) released.
  • Solution A containing the lysing agent is used for the WBC and haemoglobin (HGB) readings.
  • Solution B is used for the RBC and platelet (PLT) readings.
  • Superfrost plus slides 25 ⁇ 75 ⁇ 1 mm (Mensel-Glaser), ready for use, are adopted.
  • the blood (8 ⁇ l) is deposited on the right-hand side of the slide; another slide, placed at a 45° angle, to the left of the blood, is drawn back until in comes into contact with the drop which spreads rapidly along the contact line between the two slides; the slide is moved forwards with a smooth, rapid movement so as to obtain a thin film of blood.
  • the slide is left to dry in air, stained with Diff-Quick (DADE) dye, according to the attached instructions, and dried again in air.
  • DADE Diff-Quick
  • the slides are immersed in Histolemon solution (Carlo Erba) for 2 sec; a drop of synthetic mountant (Shandon) is deposited at the centre of the slide and a cover slide is placed over it covering the entire blood smear, taking care not to form bubbles between the two slides.
  • Histolemon solution Carlo Erba
  • Shandon synthetic mountant
  • the slides are dried and then the WBC count is performed, up to 100, with an optical microscope, after depositing a drop of cedar oil on the slide.
  • the quantity of WBC/ml, assessed using the haemocytometer, is multiplied by the percentage value of the corresponding neutrophil granulocytes of the leukocyte formula. This parameter, divided by 100, expresses the value of neutrophils/mm 3 blood.
  • Taxol treatment (30 mg/kg i.p. every 2 days for a total of 4 times) caused a significant reduction in polymorphonucleates ( ⁇ 93%, vs vehicle, p ⁇ 0.0001) in the mouse inoculated with L-MM3 murine breast cancer.
  • the oral administration of acetyl L-carnitine (100 mg/kg once daily for 10 days) combined with treatment with Taxol proved capable of significantly counteracting the Taxol-induced reduction in neutrophil granulocytes (335/mm 3 vs 65/mm 3 , oo p ⁇ 0.01) (Table 11).
  • Taxol injected according to the same schedule used for evaluation of neutrophil granulocytes (30 mg/kg i.p. every two days for a total of 4 times), was found to significantly inhibit L-MM3 tumour growth, which was monitored until the tumour size reached approximately 2 cm in the control group (0.56 cm vs 1.8 cm, p ⁇ 0.0001).
  • Combined treatment with orally administered ALC for 14 days (100 mg/kg once daily) plus Taxol (30 mg/kg i.p. every 2 days for a total of 4 times) did not affect the anticancer activity of Taxol.
  • Taxol caused severe neutropenia and ALC, administered continuously over the period in which this type of bone marrow toxicity occurs, was capable of preventing the Taxol-induced reduction in polymorphonucleates.
  • the action of ALC did not affect the anticancer activity of Taxol.
  • Taxol has proved effective in the treatment of cancer of the ovaries, breast and lungs and in other types of cancers (Rowinsky, E. K., and R. C. Donehower, (1991); Pharmacol. Ther. 52:35).
  • the anticancer action of this compound is related mainly to its ability to inhibit depolymerisation of the microtubules in tubulin monomers (Schiff, P. B., J. Fant, and S. B. Horwitz. 1979. Nature ); this effect blocks the proliferating cells in the G2/M phase of the cell cycle, i.e.
  • Taxol like other chemotherapeutic agents, moreover, is associated with side effects such as neuropathies and myelosuppression.
  • the data obtained show a statistically significant reduction in that number in the groups treated with Taxol, with Taxol in combination with acetyl L-carnitine and with vehicle in combination with acetyl L-carnitine as compared to the group treated with vehicle alone.
  • the mice treated with Taxol or with Taxol in combination with acetyl L-carnitine also showed a reduction in the diameter of the metastases compared to the groups treated with vehicle alone or with vehicle in combination with acetyl L-carnitine.
  • acetyl L-carnitine does not interfere with the anticancer action of Taxol in terms of inhibition of the tumour mass.
  • acetyl L-carnitine showed a significant inhibitory effect on the formation of lung metastases.
  • Acetyl L-carnitine inner salt (sterile ampoule, 0.5 g), solubilised in water for injectable preparations, was used.
  • solution O Each ampoule of ALC is dissolved in 4 ml of the solvent provided (solution O).
  • solution O 1.6 ml of solution (O) are brought to 40 ml with sterile buffer solution (PBS, Sigma P-4417) and then administered orally (100 mg/kg/20 ml).
  • PBS sterile buffer solution
  • Taxol (paclitaxel (INDENA), cod. 3926570) is weighed, solubilised in the specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C., sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • PBS phosphate buffer
  • Animals 60 female Balb/c mice weighing 18 g (Harlan).
  • mice 5 mice per cage; temperature 22 ⁇ 2° C.; relative humidity 55 ⁇ 15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7 ⁇ 20.7 ⁇ 14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • mice are weighed prior to the start of treatment and then once a week up to the end of the experiment.
  • the study tissue was placed in a plate containing PBS with Ca ++ and Mg ++ (Gibco) at pH 7.2 and cold, broken down into pieces measuring 2-3 mm and re-suspended in a solution (15 ml solution/g tumour) of PBS with Ca ++ and Mg ++ , pH 7.2 containing 2 mg/ml trypsin (type III, 10000 U/mg protein, Sigma-Aldrich), 2 mg/ml collagenase (type I-S 180 U/mg solid, Sigma), 0.2 mg/ml DNAse (type I 1548 U/mg protein, Sigma) and 25 ⁇ g/ml gentamicin (Sigma) and incubated at 37° C. for 15 minutes under constant stirring.
  • PBS with Ca ++ and Mg ++ Gibco
  • pH 7.2 containing 2 mg/ml trypsin (type III, 10000 U/mg protein, Sigma-Aldrich), 2 mg/ml collagenase (type I-S 180
  • the cell suspension was then homogenised with the aid of a potter (B. Braun) for 2 minutes, incubated at 37° C. for 10 minutes and aspirated gently a number of times with a syringe with a No. 21 sterile needle.
  • RPMI-1640 Eurobio
  • FBS Eurobio
  • the cell suspension was filtered on sterile gauze and then centrifuged at 700 g for 10 minutes.
  • the cell pellet was re-suspended gently in RPMI-1640 containing 10% FBS and 0.2 mg/ml DNAse (Sigma) and then centrifuged at 700 g for 10 minutes.
  • the pellet was consecutively subjected to two washings with RPMI-1640; at the end of the last washing, the pellet was re-suspended gently in RPMI-1640 so as to perform the count to establish the cell concentration.
  • the cell count is performed by means of the Trypan-Blue vital stain exclusion test; the tumour cells are suitably diluted with 0.4% Trypan-Blue (Sigma), a vital stain, which makes it possible to distinguish between viable and dead cells.
  • the dilution containing the cells to be counted was stirred gently, 10 ⁇ l were removed and used to set up a Burker chamber.
  • a square grid delimited by the three triple lines was used, comprising 16 small squares (4 ⁇ 4) delimited from one another by double lines. Both viable cells (which have a translucid appearance) and dead cells (which are blue in appearance, having incorporated the stain) which are to be found positioned inside the square formed by the median line and the triple lines, or on the line itself.
  • Inoculation conditions 60 unanaesthetised Balb/c mice weighing approximately 18 g (Harlan) received i.m. injections of 3 ⁇ 10 5 M109 lung cancer cells in 0.1 ml of RPMI-1640 (Sigma) in the right rear paw.
  • Treatment schedule for the purposes of evaluating tumour size in the experimental study groups, each consisting of 15 Balb/c mice, 3 ⁇ 10 5 M109 lung cancer cells were inoculated, and the study molecules were administered at the scheduled times.
  • ALC was administered at the dose of 100 mg/kg (os) from day 4 to day 17.
  • the vehicle diluted 1:4 with PBS from the mother solution was administered i.p. on days 8, 10, 12 and 14.
  • Taxol was administered at the dose of 30 mg/kg (i.p.) on days 8, 10, 12 and 14, as described in the following schedule:
  • the animals were kept under observation up to day 22 after inoculation of the M109 murine lung cancer cells, and were then sacrificed and the lungs removed to determine the number of metastases.
  • mice The experimental groups, each comprising 15 mice, are:
  • tumour measurement the tumour was measured with a calliper three times a week as soon as it became palpable.
  • the tumour mass is calculated on the basis of the measurements of the two dimensions (length and width), expressed in mm, according to the following formula:
  • tumour volume expressed in mm 3 is equal to the weight of the tumour expressed in mg.
  • the data obtained show a statistically significant reduction in their number in the groups treated with Taxol, with Taxol combined with acetyl L-carnitine and with vehicle combined with acetyl L-carnitine as compared to the group treated with vehicle alone.
  • the mice treated with Taxol or with Taxol combined with acetyl L-carnitine also showed a reduction in the diameter of the metastases as compared to the groups treated with vehicle alone or with vehicle plus acetyl L-carnitine (see Table 13).
  • acetyl L-carnitine has a mild inhibitory effect on the formation of lung metastases.
  • ALC does not interfere with the therapeutic effect of Taxol, and this aspect has also been evaluated in a human tumour model, as illustrated in the following example.
  • tumour was inoculated in solid fragments in both flanks of the mice (day 0).
  • the inoculated tumours were measured with a calliper and when a mean tumour weight of 100 mg was reached (day 7); the animals were divided into 4 groups of 5 animals each according to the following schedule:
  • ALC treatment was initiated and was continued for 18 consecutive days (qdx18) (groups 3 and 4).
  • ALC was administered at the dose of 100 mg/kg with an administration volume of 25 ml/kg.
  • Taxol (54 mg/kg/15 ml/kg) was administered i.v. according to a schedule consisting in a total of 4 administrations at 4-day intervals (q4dx4; days 10, 14, 18, 22) (groups 2 and 4).
  • tumour volume inhibition was calculated (TVI %, calculated as 100 ⁇ (mean weight of tumours treated/mean weight of control tumours ⁇ 100)) as obtained with the various treatments.
  • Treatment with ALC had no effect on tumour growth, which was similar to that in control group tumours.
  • the purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention and treatment, of acetyl L-carnitine administered in the coordinated use with Cisplatin.
  • Peripheral neuropathy was induced by intraperitoneally administration of Cisplatin (2 mg/kg ml) dissolved in distilled sterilized water, once a week for 9 weeks (for a total of 9 administrations).
  • Acetyl L-carnitine was administered orally at the dose of 100 mg/Kg/day.
  • ALC treatment occurred daily, starting one week before Cisplatin treatment (preventive treatment) or one week after the first Cisplatin treatment (therapeutic).
  • Sensory nerve conduction velocity was measured by means of the H reflex, to that the tibial nerve was stimulated by using a two pair of electrodes inserted subcutaneously 3 cm distally; EMG recordings from the plantar foot muscle were made with a pair of skin clips surface electrodes.
  • Cisplatin-induced neuropathy sensory conduction velocity (H reflex) in m/s measured in basal conditions and after treatment with acetyl L-carnitine.
  • the nerve conduction velocity on the caudal nerve was determined as in Example 2; data measured are given in the Table 15 here below.
  • Cisplatin-induced neuropathy sensory conduction velocity in m/s measured on the caudal nerve in basal conditions and after treatment with acetyl L-carnitine.
  • the purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention, of acetyl L-carnitine administered one day prior to Oxaliplatin treatment for 4 weeks.
  • Peripheral neuropathy was induced by intraperitoneally administration of Oxaliplatin (2.7 mg/kg/1.5 ml) dissolved in distilled sterilized water, twice a week for 4 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was dissolved in distilled water and administered subcutaneously. The dose of 100 mg/1.5 ml/Kg/day was administered on the basis of individual body weight. ALC treatment occurred daily, starting a day before Oxaliplatin treatment, for 4 weeks. Control and Oxaliplatin groups were treated s.c. with vehicle.
  • NCV Nerve conduction velocity
  • NCV was determined as in Example 2.
  • the sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 16 here below.
  • Oxaliplatin-induced neuropathy sensory nerve conduction velocity (m/s) measured on the animals' tails in basal conditions and after treatment with acetyl L-carnitine.
  • TREATMENT GROUP BASAL Oxaliplatin 8 th treatment Control 31.0 ⁇ 1.4 35.8 ⁇ 1.7 Oxaliplatin 31.3 ⁇ 1.7 30.8 ⁇ 1.2* Acetyl L-carnitine + Oxaliplatin 31.4 ⁇ 1.5 33.6 ⁇ 2.6 ⁇ Values are means ⁇ standard deviation.
  • the purpose of this study is to demonstrate and evaluate the protective properties, by way of therapy, of acetyl L-carnitine administered at the end of Oxaliplatin treatments, during a follow-up period of 3 weeks.
  • Peripheral neuropathy was induced by intraperitoneal administration of Oxaliplatin (3 mg/kg/1.5 ml) dissolved in distilled sterilized water, twice a week for 4 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was dissolved in distilled water and administered subcutaneously during the follow-up period. The dose of 100 mg/1.5 ml/Kg/day was administered on the basis of individual body weight. ALC treatment started the day after the last Oxaliplatin administration. Control and Oxaliplatin groups were treated s.c. with vehicle.
  • NCV Nerve conduction velocity
  • NCV was determined as in Example 2.
  • the sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 17 here below.
  • Oxaliplatin-induced neuropathy sensory nerve conduction velocity (m/s) measured on the animals' tails in basal conditions and after treatment with acetyl L-carnitine.
  • the paw-withdrawal test (Randall-Sellitto) was used to address mechanical hyperalgesia.
  • Nociceptive threshold was measured using an Ugo Basile analgesimeter by applying an increasing pressure to the left and right hind paws.
  • the nociceptive threshold is defined as the pressure (grams) at which the rat withdraws its paw.
  • Lower thresholds correspond to an increased sensitivity to algesia.
  • This behavioural test is conducted at different times (days) from drug or vehicle administration. Rats are made familiar with the testing procedure and with handling by investigators during the week prior to the experiments.
  • Wistar female rats (Charles River) about 10 weeks of age were used.
  • Oxaliplatin (3 mg/kg i.p.) in saline was administered three times a week. For the first two weeks, then two times a week for two weeks and then once a week.
  • the preventive treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily from 1st day of the Oxaliplatin treatment.
  • the therapeutic treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily from 24th day of the Oxaliplatin treatment.
  • the nociceptive threshold data (Randall-Sellitto) are given in the Table 18 here below.
  • the paw-withdrawal test (Randall-Sellitto) was used as in Example 15.
  • Sprague Dawley male rats (Harlan) about 3 months of age (about 350 g) were used.
  • Vinorelbine (0.200 mg/kg i.p.) in saline was administered three times a week until the end of the experiment.
  • the preventive treatment was conducted as follows: Vinorelbine 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 1st day of the Vinorelbine treatment. Treatment with ALC went until day 25.
  • the therapeutic treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 9 th day of the Vinorelbine treatment. Treatment with ALC went until day 25.
  • the test was done twice a week.
  • the nociceptive threshold data (Randall-Sellitto) are given in the Table 20 here below.
  • nociceptive threshold measured on the animals' hind paws (mean ⁇ S.D.) Days GROUP N 0 4 9 11 16 18 23 25 Saline 10 228.5 214.6 229.8 242.9 220.7 205.0 196.8 214.8 44.1 59.2 54.1 c 35.5 d 38.6 d 38.9 d 46.9 c 54.5 d Vinorelbine 12 211.4 173.5 168.8 156.8 135.8 127.7 136.2 122.7 32.9 51.6 45.6 50.5 36.0 38.2 45.4 40.9 ALC + 10 222.0 197.4 234.0 211.2 235.4 204.6 202.6 201.2 Vinorelbine 23.8 39.6 74.5 a 41.4 b 44.4 d 35.6 d 41.8 c 36.1 d Preventive - 1 day ALC + 12 216.5 167.5 160.2 190.6 196.2 227.2 209.0 206.7 Vinorelbine 44.3 41.6 60.6 70.2 49.5
  • the paw-withdrawal test (Randall-Sellitto) was used as in Example 16.
  • Sprague Dawley male rats (Harlan) about 3 months of age (about 350 g) were used.
  • Vincristine (0.150 mg/kg i.p.) in saline was administered three times a week until the end of the experiment.
  • the preventive treatment was conducted as follows: Vincristine 0.150 mg/kg i.p. three times a week+ALC 100 mg/Kg s.c., daily (6 days a week).
  • the therapeutic treatment was conducted as follows: Vincristine 0.150 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 15th day of the Vincristine treatment.
  • the test was done twice a week.
  • the nociceptive threshold data (Randall-Sellitto) are given in the Table 21 and Table 22 here below.
  • Vincristine-induced neuropathy nociceptive threshold (g) measured on the animals' hind paws (mean ⁇ S.D.) - therapeutic treatment Days GROUP N o 0 4 8 10 15 22 24 29 31 36 38 Saline 10 197.2 233.5 201.5 209.4 224.8 231.0 223.5 215.9 222.2 250.4 248.6 24.0 60.7 a 55.7 47.4 d 59.5 d 47.4 d 62.6 c 44.9 c 46.2 d 86.3 c 62.0 c Vincristine 10 199.4 173.6 145.6 131.4 127.4 120.6 133.0 128.0 129.2 137.8 154.2 25.0 64.8 71.8 31.9 50.9 40.2 74.6 56.9 46.2 52.8 64.2 ALC + 10 216.2 212.8 186.8 152.6 128.0 188.6 230.5 248.4 282.0 265.0 281.6 Vincristine 38.9 62.9 87.3 59.5 39.8 72.4 b 68.9 c 100.2
  • Pre-treatment with saline/saline IT 1. Pre-treatment with saline/saline IT 2. Pre-treatment with saline/Bleomycin IT 3. Pre-treatment with ALC/saline IT 4. Pre-treatment with ALC/Bleomycin IT
  • the lungs were fixed by insufflation at 20 cm of H 2 O ex vivo with 10% formaldehyde in PBS (pH 7), embedded in paraffin, sectioned and stained with haematoxylin/eosin. Sections with similar orientation from the portion of the pulmonary lobe—upper, middle and lower, respectively—were examined to verify the presence and degree of inflammatory infiltrate, oedema and interstitial and intra-alveolar fibrosis.
  • the lungs receiving saline or pre-treatment with ALC/saline IT presented a normal alveolar architecture.
  • In the animals pre-treated with ALC there were a number of fibrotic areas, which, however, appeared thinner with less dense areas of collapse.
  • the hydroxyproline content (one of the main constituents of collagen) was measured using the known Woessner method. Lung samples were homogenised, hydrolysed with NaOH, and then left to oxidise before adding Erlich aldehyde reagent. Duplicate aliquots of each sample were assayed by spectrophotometry and compared with a standard calibration curve obtained with purified hydroxyproline. The hydroxyproline content (HYP) is expressed as mg/lung.
  • ALC causes a reduction of the fibrotic response in animals treated with Bleomycin.
  • L-MM3 murine breast cancer cells were used, cultured at 37° C. in plastic flasks in a humidified atmosphere with 5% CO 2 .
  • the cells were grown in DMEM supplemented with 10% FCS and in the presence of 2 mM L-glutamine and 80 ⁇ g/ml of gentamicin.
  • the subconfluent cells were collected during the exponential growth phase using trypsin-EDTA and re-suspended in DMEM. They were then injected subcutaneously in female Balb/c mice weighing 20 g at a density of 4 ⁇ 10 5 .
  • tumour mass is calculated on the basis of the measurements of the two dimensions (length and width), expressed in mm, according to the following formula:
  • Taxol paclitaxel INDENA
  • the agent is weighed, solubilised in the specific vehicle (12 mg/ml), and stored at +4° C., sheltered from the light. At the time of use, it is diluted 1:4 with saline solution in phosphate buffer (PBS, SIGMA) and injected.
  • PBS phosphate buffer
  • Agent Vehicle Cremophor EL (BASF).
  • Cremophor is diluted 1:1 with ethanol and stored sheltered from the light. On the day of treatment it is diluted 1:4 with PBS.
  • mice were treated i.p. with 30 mg/kg of Taxol and s.c. with 100 mg/kg of PLC according to the following schedule.
  • Taxol treatment was given according to the same procedures and at the same times both in the group treated with Taxol alone and in the one treated with Taxol and PLC.
  • Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection. Cells were cultured in RPMI (Sigma Chemicals, Aldrich) supplemented with 5% heat-inactivated horse serum (Gibco, Milan, Italy), 5% heat-inactivated foetal calf serum and 1% PNS antibiotic mixture (Gibco, Milan, Italy) in a humidified incubator (Heraeus, Angelantoni, Italy) at 37° C. with 5% CO 2 atmosphere. PC12 cells were seeded in 6 wells plates (containing a polysilylated cover slide) at the density of 150.000 cells/well. After 6 hrs cells were treated with NGF 100 ng/ml for 72 h to induce neuronal differentiation and neurite outgrowth.
  • RPMI Sigma Chemicals, Aldrich
  • the medium was replaced and the differentiated cells were treated for 24 h with 100 ng/ml NGF+/ ⁇ Taxol (1 ⁇ g/ml) or cisplatin (10 ⁇ g/ml)+/ ⁇ ALC (1 mM).
  • PC12 cells were then fixed using the methanol-acetone method: cells grown on the cover slides were washed twice with PBS 1 ⁇ , then treated with chilled methanol ( ⁇ 20° C.) and incubated at +4° C. for 2 min. Then, methanol was flushed out and chilled acetone ( ⁇ 20° C.) was put on to the cells.
  • the percentage of neurites of differentiated cells was drastically decreased in Taxol-treated cells with respect to the controls (7% vs. 39%, respectively p ⁇ 0.001).
  • the reduction in the percentage of differentiated cells was evident also in the cisplatin-treated cells vs. controls, although to a lesser extent (19% vs. 39% respectively; p ⁇ 0.01).
  • the ALC co-treatment with Taxol or cisplatin reduced the degeneration of the elongated neurites (differences were not significant respect to the control group). No differences between the controls and ALC treated cells were observed.
  • the purpose of this study is to evaluate NGF circulating levels in relation to Cisplatin induced peripheral neuropathy, and to assess the effect of acetyl L-carnitine administered in the co-ordinated use with Cisplatin.
  • Peripheral neuropathy was induced by intraperitoneally administration of Cisplatin (2 mg/kg ml) dissolved in distilled sterilized water, once a week for 8 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was administered orally at the dose of 100 mg/Kg/day. ALC treatment occurred daily, starting 5 days before Cisplatin.
  • the nerve conduction velocity on the caudal nerve was determined in baseline condition and at the end of the treatment period as in Example 2; data measured are given in the Table 24 here below.
  • Cisplatin-induced neuropathy sensory conduction velocity in m/s measured on the caudal nerve in basal conditions and after treatment with acetyl L-carnitine.
  • NGF circulating levels were determined: 3 ml of whole blood were obtained from each animal through abdominal aorta puncture and collected into a heparinated tube. Plasma was obtained and immediately stored at ⁇ 80° until used for NGF level determination. The circulating levels of NGF were measured by ELISA using commercially available kits (EmaxTM ImmunoAssay System, Promega, USA; plasma working dilution for NGF 1:80) following the manufacturer protocols. NGF circulating levels are given in the Table 25 here below.

Abstract

A method for preventing and/or treating peripheral neuropathies induced by the administration of an anticancer agent of the family of platin compounds, taxanes, epothilone class, vinca alkaloids, said method comprising the administration in a co-ordinated manner to a subject suffering from said peripheral neuropathies, or expected to suffer from said peripheral neuropathies, an effective amount of acetyl L-carnitine or of a pharmaceutically acceptable salt thereof. In case of prevention, acetyl L-carnitine is administered to a subject, in view of the need of a treatment with an anticancer agent, immediately before or immediately after surgical removal of the tumor, but in any case before the administration of the anticancer agent. Acetyl L-carnitine can enhance the supportive effect of physiological NGF during chemotherapy-induced neuropathy, thus avoiding the problem of the local and general side effects of the exogenous administration of NGF which are a major problem of this neuroprotective strategy.

Description

  • This application is a continuation-in-part of application Ser. No. 09/769,488, which is a continuation of PCT/IT99/00242.
  • The invention described herein relates to the use of L-carnitine and alkanoyl L-carnitines in the preparation of medicaments useful in the treatment of tumours, particularly in combination with anticancer agents.
  • BACKGROUND TO THE INVENTION
  • It is well-known that the use of anticancer agents in human therapy causes a large number of toxic or side effects which may be life-threatening for the patients. These complications, in fact, may lead to a reduction in the doses of the agents, and occasionally to discontinuation of the therapy itself.
  • Reduction of the dose or discontinuation of the therapy in many cases causes a deterioration of the individual's general condition because it favours the development of relapses, with consequences which are sometimes fatal for the patient.
  • Another very important and strongly felt aspect in the hospital setting and among the families of oncological patients is the concept of “improving the quality of life” of the patients under treatment.
  • It is equally well known that patients undergoing regular polychemotherapy for cancer are subject to a substantial weight loss.
  • The growing number and importance of the anticancer agents used in human therapy, the main limitation of which continues to be the occurrence of toxic or side effects, mean that this problem is still a matter for considerable concern.
  • Thus, the discovery of new agents or new, appropriate combinations of different agents capable of substantially reducing the toxic or side effects caused by anticancer agents used in human therapy is much to be desired.
  • Previous uses of L-carnitine in combination with anticancer agents are already known.
  • In experimental animal models, it has been demonstrated that rats treated with doxorubicin alone show a greater weight loss than a group of rats treated with the same substance in combination with L-carnitine (Senekowitsch R, Lohninger A, Kriegel H., Staniek H., Krieglsteiner H P., Kaiser E. Protective effects of carnitine on adriamycin toxicity to heart. In: Kaiser E., Lohninger A., (eds.). Carnitine: its role in lung and heart disorders 126-137. Karger, Basel-New York, 1981).
  • U.S. Pat. No. 4,713,370 describes the use of carnitine in combination with cytostatic agents such as daunomycin, N-acetyl-daunomycin and daunomycin oxime to reduce the cardiac toxicity of these compounds. U.S. Pat. No. 4,267,163 describes the use of carnitine in combination with cytostatic agents such as adriamycin, adriamycin-14-octanoate, 4′-epi-adriamycin, adriamycin beta-anomer and 4′-epi-adriamycin gamma-anomer to reduce the cardiac toxicity of these compounds. U.S. Pat. No. 4,751,242 describes the use of acetyl L-carnitine for the therapeutic treatment of peripheral neuropathies. In this latter patent, peripheral neuropathies are defined as a group of persistent disturbances of the motor neurons of the brain stem and spinal cord and/or the primary sensory neurons and/or the peripheral autonomic neurons, with involvement of the peripheral axons and their attendant supporting structures. With regard to their etiology, peripheral neuropathies constitute a heterogeneous class of diseases because their etiology may be secondary to viral infections (herpes zoster), ischaemia (arteriosclerosis), metabolic unbalances (diabetes, renal and liver insufficiency), drug-induced toxicity (adriamicine, isoniazide, nitrofurantoin), mechanical stresses (compression, entrapment, fracture or dislocation of bones), radiations, genetic factors and pathologies of the immune system. However, no matter what the actual etiological cause of the disease form is, it is always possible to detect an alteration in the membrane fluidity resulting from an alteration of the cell lipids, cholesterol and gangliosides. Lipids play a very important role in defining the tertiary and quaternary protein structure and in maintaining the stability of adenosintriphosphatase structure. In fact, their absence brings about the enzyme inactivity. In that reference, it is postulated that acetyl L-carnitine is likely to perform a “scavenger” effect on the free radicals (superoxide, hydroperoxide) that form in conditions of irregular or insufficient perfusion or during phlogistic processes because of the decreased activity of cytochrome oxidase that controls the metabolic shift between the tetravalent and the bivalent anaerobic route. Acetyl L-carnitine, by increasing the levels of reduced glutathione, one of the most important antioxidant metabolites, and probably the levels of the cytochrome oxidases, might bring about the free radicals scavenge and the restoration of the tetravalent oxido-reductant respiratory mechanism in general and of the neurons in particular. Moreover, acetyl L-carnitine would affect the neurons by bringing about an increase in ATP-ase and AChE that are indispensable for optimum neurotransmission.
  • Other studies have addressed the evaluation of the protective effects of carnitines on anthracycline-induced cardiac toxicity (Neri B., Comparini T., Milani A., Torcia M., Clin. Trial J. 20, 98-103, 1983, De Leonardis V., De Scalzi M., Neri B., et al. Int. J. Clin. Pharm. Res. 70, 307-311, 1987).
  • The patent and bibliographical references cited above demonstrate that many efforts have been made in an attempt to reduce the toxic or side effects of anticancer agents, without, however, solving this serious problem in a satisfactory manner.
  • Carboplatin is a structural analogue of Cisplatin and its associated nephrotoxicity, though by no means negligible, is less than that of Cisplatin. Peripheral neurotoxicity is the dose-limiting side effect of the antineoplastic drug Cisplatin (Cavaletti et al. Journal of the Peripheral Nervous System, Vol. 6, Number 3; September 2001, 136-2001 Meeting of the Peripheral Nerve Society).
  • Vincristine is a well-known anticancer agent which has toxic effects, particularly at the level of the immune system.
  • Taxol is a natural extract, first isolated from the bark of Taxus brevifolia, with anticancer properties and has proved neurotoxic and myelotoxic in human subjects. It is used for the treatment of tumours resistant to platinum therapy, but gives rise to greater cumulative toxicity in the peripheral nervous system. It has also been ascertained that Taxol induces neutropenia in the subjects treated (Rowinsky et al.; Semin. Oncol. (1993), Aug. 20 (4 suppl 3), 1-15; Onetto et al. J. Natl. Cancer Inst. Monogr. (1993); (15):131-9).
  • Bleomycin is typically used in young patients with testicular cancer, lymphoma, and other types of tumours. The pulmonary toxicity of Bleomycin is characterised by destruction of the alveolar epithelial barrier and the consequent intra-alveolar proliferation of fibroblasts and deposition of extracellular matrix components (Karam H et al.; Cell Biol. Toxicol (1998 February); 14(1):13-22). Type 2 pneumocytes are not capable of regenerating the damaged or lost epithelium.
  • Different classes of chemotherapeutic drugs induce peripheral neurotoxicity: the taxoids, the platinum compounds, the vinca alkaloids and the epothilone class.
  • Severe peripheral neuropathy can induce therapy modification, while mild or moderate cases still represent an important tolerability problem limiting patient quality of life.
  • Whereas the vinca alkaloids bind to tubulin and prevent the polymerisation from soluble dimers into microtubules, the taxoids promote the formation of microtubules and prevent their depolymerisation, which results in an abundance of rigid microtubules.
  • Microtubules are essential components for the maintenance of cell shape, a variety of cellular actions and axoplasmic transport. Defective function of microtubules in neurons and axons may be the origin of the neurotoxicity of both families of chemotherapeutic agents.
  • The taxoids induce primarily a symmetrically distributed sensory distal neuropathy, which is related to both single and cumulative doses of the drug and is possibly dependent on the regimen. The neurotoxicity of Taxol is due to a unique mechanism of action; it binds to tubulin and promotes microtubule assembly (Schiff P. B. et al., Nature 1979; Parnass J. and Horowitz S. B., J. Cell. Biol. 1981). Microtubules formed in the presence of Taxol are stable in conditions which ordinarily depolymerize them, including the presence of calcium and cold. Paclitaxel also alters the kinetics of microtubule assembly, eliminating the 3- to 4-minute lag time normally observed prior the initiation of assembly (Horowitz S. B. et al., Ann. N.Y. Acad. Sci. 1986). This mechanism contrasts with that of the vinca alkaloids which inhibit microtubule assembly.
  • This contrasting mechanism makes difficult to find a drug acting at the same time on the two differently induced neuropathy.
  • With the use of hemopoietic growth factor rescue of myelotoxicity, neurotoxicity becomes the dose-limiting factor (Schiller et al., J. Clin. Oncol. 1994). Symptoms may begin as early as 24 to 72 hours after treatment with high single doses (>250 mg/m2), but the neurotoxicity is typically cumulative, with symptoms progressing after each treatment at both high and low doses.
  • The symptoms are generally tolerable (NCI grade 1 or 2). The neurologic examination characteristically reveals an elevated threshold and perception of vibration in a distal, symmetric, glove-and-stocking pattern. In general, large-fiber modalities (vibration, proprioception) are more frequently affected than loss of small-fiber modalities (pain, temperature). Deep tendon reflexes are also frequently affected, with the distal (ankle) reflex being invariably absent or reduced.
  • Nerve conduction studies show reductions of sensory nerve action potential amplitudes in a symmetric, distal, length-dependent fashion. Sural sensory nerve action amplitude is virtually always reduced or absent in symptomatic patients.
  • Mild sensory symptoms have usually improved or resolved completely within several months after discontinuation of Taxol.
  • Cisplatin induces a peripheral sensory axonal neuropathy, affecting large-diameter and, to a lesser extent, small-diameter sensory fibres. Paraesthesias and impaired propiocepsis are the most common symptoms (Thompson S. W. et al., Cancer 1984). Cisplatin accumulates in and damages the dorsal root ganglia, axonal changes being secondary to neuronal damage (Warner E., Int. J. Gynecol. Cancer 1996).
  • The incidence of Cisplatin-induced neuropathy depends largely on the cumulative dose, the onset of symptoms being seen at a total dose of Cisplatin 330 to 400 mg/m2. Cisplatin binds tightly and irreversibly to nerve tissue, which explains the deterioration of neurological condition which sometimes occurs after cessation of Cisplatin therapy.
  • The vinca alkaloids induce a peripheral sensory-motor polyneuropathy and autonomic neuropathy, which appears to be partially reversible after some months (Potsma T. J. et al., J. Neurooncol. 1993).
  • The epothilone family has a toxicological profile similar to the taxanes one (Lee F Y et al Proc Am Assoc Cancer Res, 2002, 792).
  • One of the general problems of pharmacological therapy is the therapeutic index of the agents, that is to say the ratio of the therapeutically effective dose to the toxic dose, or, at any rate, the dose that gives rise to the onset of side effects.
  • The medical community still perceives the need for therapeutic regimens which allow the patient to face up to the treatment, which, in the case of anticancer chemotherapy is particularly hard to support, while at the same time conserving an acceptable quality of life. These considerations also apply to the therapeutic treatment of animals, for instance, so-called pets.
  • The natural tendency to reduce the doses, and thus the use of pharmaceutical forms suitable for therapeutically useful administrations without obliging the patient to take the agents too often, contrasts with the minimum effective doses typical of each anticancer agent.
  • SUMMARY OF THE INVENTION
  • Surprisingly, it has been found that the co-ordinated use—this term will be precisely defined below—of an anticancer agent and L-carnitine or an alkanoyl L-carnitine, as defined below, exerts an unexpected synergistic effect on the activity of the anticancer agent.
  • In the context of the invention described herein, it has also been found, in an entirely unexpected way, that the co-ordinated use of a therapeutically effective amount of a peripheral neuropathy-inducing anticancer agent, in particular of the taxane, platin, epothilone and vinca alkaloid families and Bleomycin, in particular Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine, and Vincristine, and a detoxifying amount of L-carnitine or of an alkanoyl L-carnitine, in which the linear or branched alkanoyl has 2-8 carbon atoms, or one of its pharmacologically acceptable salts, affords a potent protective effect against the toxicity and side effects of the anticancer agent, without impairing its efficacy, thus giving rise, amongst other things, to a substantial improvement in the quality of life and a prolonging of life itself in the subjects treated, whether human subjects or animals.
  • It is a further particular object of the present continuation-in-part a method for preventing and/or treating peripheral neuropathies induced by the administration of an anticancer drug of the family of platin compounds, taxanes, epothilone class and vinca alkaloids, said method comprising administering in a co-ordinated manner to a subject suffering from said peripheral neuropathies, or expected to suffer from said peripheral neuropathies, an effective amount of acetyl L-carnitine of a pharmaceutically acceptable salt thereof.
  • It has also been found that said co-ordinated use has an inhibitory effect on tumour metastases.
  • Therefore one object of the invention described herein is the use the compound of formula (I):
  • Figure US20090143464A1-20090604-C00001
  • where R is hydrogen or an alkanoyl group with 2 to 8 carbon atoms, and X represents the anion of a pharmaceutically acceptable salt, for the preparation of a medicament comprising an anticancer agent, characterised in that said compound produces a synergistic effect on the activity of the anticancer agent.
  • Also an object of the invention described herein is the co-ordinated use of the compound of formula (I) according to which the side effects of the anticancer agent in said medicament are substantially reduced.
  • A further object of the invention described herein is the use the compound of formula (I) in the preparation of a medicament useful for inhibiting metastases.
  • Yet another object of the invention described herein are combinations of the compound of formula (I) with anticancer agents and the related pharmaceutical compositions.
  • The well known lack of toxic or side effects of carnitine and alkanoyl L-carnitines makes the use of these compounds particularly safe even for long periods of treatment, for the prevention or treatment of toxic or side effects, such as weight loss, heart, kidney and central nervous system damage, peripheral nervous system damage, particularly neuropathy or neutropenia caused by Taxol, or lung damage induced by Bleomycin, even more particularly peripheral neuropathy caused by Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine.
  • The implementation of the invention described herein also contributes to improving the quality of life of the patients treated; one need only think of the physical suffering caused by peripheral neuropathy, neutropenia, respiratory complications or debilitation due to weight loss caused by these agents.
  • These and other objects of the invention described herein will be described in detail in the embodiment forms of the invention, also by means of examples.
  • In the context of the invention described herein, the terms “antineoplastic”, “anticancer” and “antiproliferative” are to be understood as being essentially synonymous.
  • DETAILED DESCRIPTION OF THE INVENTION
  • In the context of the invention described herein, what is meant by “co-ordinated use” of the aforesaid compounds is, indifferently, either (i) co-administration, i.e. the substantially simultaneous or sequential administration of L-carnitine or an alkanoyl L-carnitine or one of its pharmacologically acceptable salts and of an anticancer agent, or (ii) the administration of a composition comprising the aforesaid active ingredients in combination and in a mixture, in addition to optional pharmaceutically acceptable excipients and/or vehicles.
  • The invention described herein thus covers both the co-administration of L-carnitine or an alkanoyl L-carnitine or one of its pharmacologically acceptable salts of formula (I) and of the anticancer agent, and pharmaceutical compositions, which can be administered orally, parenterally or nasally, including controlled-release forms, comprising the two active ingredients in a mixture. Preferably, the alkanoyl L-carnitine is selected from the group consisting of acetyl L-carnitine (hereinafter abbreviated to ALC or Alcar), propionyl L-carnitine (hereinafter abbreviated to PLC), butyryl L-carnitine, valeryl L-carnitine and isovaleryl L-carnitine, or one of their pharmacologically acceptable salts. The ones preferred are acetyl L-carnitine, propionyl L-carnitine and butyryl L-carnitine. The most preferred is acetyl L-carnitine.
  • Though clear from the following detailed description of the invention, one can also envisage the co-ordinated use of an anticancer agent, such as Taxol, acetyl L-carnitine and propionyl L-carnitine, or of Bleomycin and acetyl L-carnitine, or—a further possibility—of acetyl L-carnitine and Taxol or Cisplatin or Carboplatin or Oxaliplatin or Epothilone or Vincristine or Vinorelbine. In all these embodiments, the most preferred acetyl L-carnitine can be used in the co-ordinated use.
  • The intended use of the co-administration in the context of the present invention means the possibility to perform a preventive treatment (intended also as prevention) for the possible adverse effect of the anticancer agent. In another embodiment, by the intended use of co-administration, the present invention means the therapeutic treatment (intended also as treatment or therapy) of the adverse effect of the anticancer agent. In the case of preventive treatment the alkanoyl L-carnitine, preferably acetyl L-carnitine, shall be administered in view of the need of a treatment with an anticancer agent, known to imply adverse effects, in particular peripheral neuropathy. The start of the administration, the subsequent schedule (namely, the posology) and the decision upon continuing the administration even after the start of the treatment with the anticancer agent shall be within the knowledge of the clinical expert, depending also on the kind of anticancer agent, the type and severity of the foreseen adverse effect, the conditions of the patient. Just for sake of example, the administration of the alkanoyl L-carnitine, preferably acetyl L-carnitine can be started immediately before or immediately after surgical removal of the tumor, but in any case before the start of the administration of the anticancer agent, also in the case the agent be administered before surgery. In case the administration of the anticancer agent is foreseen before, or alternatively to, surgery, both prevention and treatment may apply, whenever the case.
  • Co-administration also means a package, or manufactured article, comprising distinct administration forms of L-carnitine or one of the aforesaid alkanoyl L-carnitines, or one of their pharmacologically acceptable salts and of an anticancer agent, accompanied by instructions for the co-ordinated simultaneous or time-scheduled intake of the active ingredients according to a dosage regimen established by the primary care physician, on the basis of the patient's condition. This package is suitable both for prevention and treatment. In a different embodiment of the present invention, the package or manufactured article comprises a unit dosage containing both the anticancer agent and the alkanoyl L-carnitine, preferably acetyl L-carnitine.
  • What is meant by a pharmacologically acceptable salt of L-carnitine or of an alkanoyl L-carnitine is any salt of the latter with an acid that does not give rise to toxic or side effects. These acids are well known to pharmacologists and to experts in pharmaceutical technology.
  • Examples of pharmacologically acceptable salts of L-carnitine or of the alkanoyl L-carnitines, though not exclusively these, are chloride; bromide; iodide; aspartate; acid aspartate; citrate; acid citrate; tartrate; acid tartrate; phosphate; acid phosphate; fumarate; acid fumarate; glycerophosphate; glucose phosphate; lactate; maleate; acid maleate; mucate; orotate, oxalate; acid oxalate; sulphate; acid sulphate; trichloroacetate; trifluoroacetate; methane sulphonate; pamoate and acid pamoate.
  • One preferred form of daily dosing of L-carnitine or alkanoyl L-carnitine for clinical use is a composition comprising an amount of L-carnitine or an alkanoyl L-carnitine, preferably acetyl or propionyl L-carnitine, equivalent to 0.1 to 3 g, and preferably 0.5 to 3 g.
  • The invention described herein is advantageous in the prevention or treatment of toxic or side effects such as weight loss, heart, kidney and central nervous system damage, peripheral nervous system damage, peripheral neuropathy and particularly the myelosuppression and lung damage caused by the above-mentioned anticancer agents.
  • What is meant by substantially protective effect is the prevention, reduction or elimination or anyway treatment of the side effect to a statistically significant extent.
  • The embodiment of the invention described herein also contributes to healing and to prolonging the lives of the patients thanks to the increase in therapeutic success due to the possibility of maintaining the scheduled treatment protocols or of increasing the doses of the chemotherapeutic agent, without having to discontinue the treatment due to contraindications.
  • A further benefit which is obtained with the invention described herein is related to the quality of life of the subjects treated; in fact, as already mentioned, the elimination or reduction of the physical suffering caused by a peripheral neuropathy or by debilitation due to weight loss favours the patient's ability to be self-sufficient. From the economic standpoint, there are obvious savings in terms of the costs borne by hospital facilities or by the families for the patient's care.
  • Myelosuppression is one of the toxic side effects that may manifest themselves as a result of administration of Taxol, a chemotherapeutic agent used in the therapy of various tumours, for example, of the breast, ovaries, lungs (small cell and otherwise), head and neck (Slichenmeyer and Von Hoff; J. Clin. Pharmacol. (1990), 30, 770-778). The vehicle adopted for Taxol, also in the commercial pharmaceutical forms (TAXOL®, Bristol Myers Squibb), is a derivative of polyethoxylated castor oil, known commercially as Cremophor® EL and is capable of inducing histamine release and anaphylactoid reactions in the dog and in human subjects (Slichenmeyer and Von Hoff; ibid; Bury et al.; Allergy (1992), 47, 624-629; Hershkoviz et al.; J. Leukoc. Biol. (1994), 56, 495-501; Inokuma et al.; J. Vet. Med. Sci. (1994), 56, 45-49). In view of the fact that the marketed drug is vehicled in Cremophor® EL, the problem of the myelotoxicity relating to the preparation used in therapy has been tackled.
  • One of the most serious problems encountered in the course of proliferative diseases is the metastatic spread of the tumour, which sometimes advances to such an extent as to render useless the treatment of the primary tumour and itself becomes the cause of death.
  • In a first preferred embodiment of the invention described herein, L-carnitine, combined with an anticancer agent such as Taxol, Carboplatin or Vincristine, ensures an extension of survival of the subject treated.
  • In a second embodiment of the invention described herein, acetyl L-carnitine has shown an unexpected degree of protective activity against side effects induced by one of the anticancer agents Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine, much particularly peripheral neuropathy, but also myelosuppression and weight loss. This protective effect shall be understood both as prevention of said side effect and as treatment of said protective effect.
  • In the particular and preferred embodiment of the present invention relating to the protective activity of acetyl L-carnitine against peripheral neuropathy induced by anticancer agents of the taxane, platin and vinca alkaloid families, although the present inventors do not wish to be bound to theoretical speculations, it shall be underlined how the said protective activity (both preventive and therapeutic) by acetyl L-carnitine appeared totally unexpected on the basis of the knowledge in the art. As discussed in the Background section, it is postulated that acetyl L-carnitine is likely to perform a “scavenger” effect on the free radicals by increasing the levels of reduced glutathione. In that section there are also explained the mechanisms by which the anticancer drug of interest in the present invention damage the peripheral nerve. In the case of platin family, it is also well-known that radical scavenger agents are likely to affect drug activity, since it acts through a radicalic mechanism, so the use of a radical scavenger is not advisable.
  • In the context of the present invention, it has been found in a totally surprising manner that acetyl L-carnitine exerts its protective effect against peripheral neuropathy induced by the anticancer drugs of interest herein by potentiating the effect of Nerve Growth Factor (NGF).
  • NGF is extensively studied in the art as a neuroprotective agent in relation to Cisplatin and Taxol neurotoxicity. The findings of the present invention indicate that acetyl L-carnitine is able to potentiate the effect of NGF on PC12 cells by enhancing expression of different genes, as assessed by microarray analysis, and a relationship between NGF and acetyl L-carnitine in promoting PC12 neuritis outgrowth was also reported. Another definite relationship between acetyl L-carnitine and NGF was found even in the animal model of chronic Cisplatin-induced sensory neuropathy. In this model, a close relationship has already been previously demonstrated between the onset of a sensory neuropathy and the decrease in NGF circulating levels. According to the present invention, acetyl L-carnitine co-treatment significantly reduces the severity of Cisplatin-induced sensory neuropathy and, accordingly, the circulating levels of NGF are significantly higher in the acetyl L-carnitine co-treated subjects than in Cisplatin-treated ones. These data are in agreement with the previously reported results which in different animal models demonstrated that the exogenous administration of NGF was able to reduce the severity of Cisplatin-induced peripheral neurotoxicity.
  • A significantly reduced severity of neuropathy was also observed in the in vivo model of Taxol neurotoxicity in the in Taxol-acetyl L-carnitine treated group. In addition to the effect in reducing the NGF fall in Cisplatin treated animals, acetyl L-carnitine seems to increase the cells response to NGF. Concerning the molecular mechanisms at the basis of the protective effect of acetyl L-carnitine, the studies performed in the present invention showed the ability of the protective agent to enhance neuronal NGF response via histone acetylation, which is involved in regulation of gene expression. The acetyl group of acetyl L-carnitine is transferred to histones in NGF-differentiated PC12 cells. The presence of acetyl L-carnitine increased NGF-induced histone acetylation. Moreover, the addition of acetyl L-carnitine to PC12 cells significantly stimulates the expression of NGFI-A, a gene coding for a transcription factor with tumor suppressor effects. In addition, NGFI-A protein is implicated in several physiological processes and it was suggested to have an important role in tissue repair. In conclusion, the present invention indicates that acetyl L-carnitine is a specific protective agent for chemotherapy-induced neuropathy following treatment with the anti-cancer agents of interest in the present invention. Relevant to this point is the lack of any interference with the antitumor activity of the drugs. Finally, acetyl L-carnitine can enhance the supportive effect of physiological NGF during chemotherapy-induced neuropathy, thus avoiding the problem of the local and general side effects of the exogenous administration of NGF which are a major problem of this neuroprotective strategy.
  • In a third embodiment of the invention described herein, acetyl L-carnitine has shown surprising antimetastatic activity when administered concomitantly with Taxol, particularly in lung cancer.
  • According to another preferred embodiment of the invention described herein, propionyl L-carnitine has shown an unexpected synergistic effect in combination with Taxol.
  • It has been found that Taxol induces severe neutropenia with a nadir after the 3rd-4th injection of the compound.
  • When ALC is used according to the invention described herein, no adverse effects on the anticancer action of the drug are found.
  • ALC can be conveniently administered orally, without, for that reason, excluding other administration routes which an expert in the field may deem it advisable to adopt, particularly, by injection, to be administered concomitantly, for example, in the same infusion vial, with the anticancer agent, or in sequence, as established by the expert in the field.
  • Equally, propionyl L-carnitine (PLC) has shown a synergistic effect with the therapeutic activity of Taxol.
  • It is therefore evidently advantageous to provide a binary combination, also in separate dosage forms, or in some way combined, of acetyl L-carnitine as a protective agent and one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine. This combination can also comprise other anticancer agents which induce substantially reduced side effects, much particularly peripheral neuropathy, as a result of the combination according to the invention described herein. This latter embodiment is advantageous in case of polychemotherapy. It may also be advantageous to add L-carnitine to the above-mentioned combination.
  • It is therefore further evidently advantageous to provide a ternary combination, also in separate dosage forms, or in some way combined, of acetyl L-carnitine as a protective agent, propionyl L-carnitine as a synergistic agent and one of the anticancer agents select selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine. This combination also comprises other anticancer agents which show a synergistic effect or induce substantially reduced side effects as a result of the combination according to the invention described herein. This latter embodiment is advantageous in case of polychemotherapy. It may also be advantageous to add L-carnitine to the above-mentioned combination.
  • One specific object of the invention described herein is a pharmaceutical composition comprising a therapeutically effective amount of one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine together with a protective amount of acetyl L-carnitine and a synergistic amount of propionyl L-carnitine, in a mixture with pharmaceutically acceptable vehicles and/or excipients.
  • In a different embodiment, it is in any case advantageous to provide a binary combination of acetyl L-carnitine, as a protective agent, and Bleomycin.
  • As regards those aspects relating to industrial applicability, the invention described herein also provides, in one of its possible embodiments, for a kit containing a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine, as defined above, in an amount suitable for producing a synergistic effect with said anticancer agent; c) a pharmaceutical combination comprising at least one alkanoyl L-carnitine and/or L-carnitine, as defined above, in an amount suitable for producing a substantially protective action against the side effects of said anticancer agent. The kit according to the invention described herein may also be presented in the form of a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine in an amount suitable for producing a synergistic effect with said anticancer agent. Alternatively, the kit according to the invention described herein may also be presented in the form of a) a pharmaceutical composition comprising a therapeutically effective amount of an anticancer agent; and b) a pharmaceutical composition comprising at least one alkanoyl L-carnitine and/or L-carnitine in an amount suitable for producing a substantially protective action against the side effects of said anticancer agent.
  • Specific examples of the above-mentioned kits refer to one of the anticancer agents selected from the group consisting of Taxol, Carboplatin, Cisplatin, Oxaliplatin, Epothilone, Vinorelbine and Vincristine and Bleomycin as the anticancer agents.
  • We shall now disclose the ways of implementing the invention described herein with reference to the preferred embodiments, using Taxol or Cisplatin or Oxaliplatin or Epothilone or Vinorelbine as the anticancer agent, acetyl L-carnitine as the substantially protective agent and propionyl L-carnitine as the substantially synergistic agent.
  • It remains understood that the expert in the field may complete the experimental protocols with his or her own general knowledge of the field in which he or she operates, possibly resorting to neighbouring sectors in case of need.
  • We report here below the results of the most significant experiments suitable for demonstrating the unexpected and surprising protective effect obtained by the combination of L-carnitine or its derivatives with the above-mentioned anticancer agents.
  • EXAMPLE 1 Variations in Survival Time in Rats Treated with Anticancer Agents
  • The purpose of this experiment is to demonstrate and evaluate the protective effect expressed as an increase in survival time, induced by L-carnitine in a murine experimental model.
  • Groups of 10 male Wistar rats aged 3 months (Charles River) were used, housed at 22±2° C., with 50±15% relative humidity and a 12 hour light/darkness cycle, maintained with water and feed “ad libitum”.
  • The substances used were: L-carnitine, Taxol, Carboplatin, and Vincristine.
  • The rats were treated with the anticancer agents intravenously (i.v.) at the doses corresponding to their respective LD30, LD50 and LD80.
  • The treatments with L-carnitine, 200 mg/kg, were administered subcutaneously once daily, starting 8 days prior to administration of the anticancer agent and continuing for another 14 days.
  • The mortality of the rats, identified immediately prior to treatment by means of progressive numbers on their tails, was monitored daily for 14 days after administration of the anticancer agent; the experimental data were evaluated using the Wilcoxon and Log-Rank tests and the statistical significance obtained in the evaluation of the experimental data is reported in Table 1 here below.
  • TABLE 1
    Evaluation of survival time of rats treated with L-carnitine and
    anticancer agent at three different dosage levels.
    AMOUNT OF ANTICANCER
    AGENT ADMINISTERED
    CORRESPONDING TO:
    LD80 LD50 LD30
    Compound (high dose) (medium dose) (low dose)
    Carboplatin + P < 0.05 P < 0.01 P < 0.43
    L-carnitine (p < 0.08) (p < 0.06) (p < 0.54)
    Taxol + P < 0.02 P < 0.51 P < 0.15
    L-carnitine (p < 0.05) (p < 0.42) (p < 0.15)
    Vincristine + P < 0.08 P < 0.02 P < 0.49
    L-carnitine (p < 0.04) (p < 0.12) (p < 0.73)
  • The significance refers to the combination versus the respective control (anticancer agent alone at the same dose)
  • The results obtained, reported in Table 1, show a significant lengthening of survival time in the groups treated with L-carnitine and anticancer agent.
  • The results of the statistical analysis reported in Table 1 show two “p” values. The first was calculated using the Wilcoxon test; the second, i.e. the “p” value in brackets, was calculated using the Log-Rank test.
  • The discrepancies between the results of the two tests, the Wilcoxon and the Log-Rank test, are due to the fact that the former is more powerful in detecting differences in the first part of the survival curve, and the latter in the second part.
  • In the experiment performed, the differences in survival times occur mainly in the first part of the curve.
  • The lack of statistical significance in the comparisons between the lower doses may be explained by the fact that, owing to the low number of deaths, the tests are not very powerful in detecting differences between groups.
  • EXAMPLE 2 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Taxol-Induced Peripheral Neuropathy—Prevention Treatment
  • The purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention, of acetyl L-carnitine administered one week prior to Taxol at two different doses of the latter (16 mg/kg and 8 mg/kg), by measuring the sensory nerve conduction velocity (SNCV), determined on the tail.
  • Female Wistar rats aged 3 months (Charles River) were used, housed at 22±2° C., with 50±15% relative humidity and a 12 hour light/darkness cycle.
  • The rats were identified immediately prior to treatment by means of progressive numbers on their tails and were maintained with water and feed “ad libitum”.
  • The substances used were acetyl L-carnitine and Taxol.
  • The following experimental groups were formed:
  • 1. Controls.
  • 2. Sham (group receiving Taxol solution solvent).
    3. Taxol 16 mg/kg.
    4. Acetyl L-carnitine+Taxol 16 mg/kg.
    5. Taxol 8 mg/kg.
    6. Acetyl L-carnitine+Taxol 8 mg/kg.
  • The following treatment schedule was used: the Sham animals received Taxol solution solvent (cremophor/ethanol) intraperitoneally (i.p.); Taxol was administered i.p. once a week for 5 weeks; the treatments with acetyl L-carnitine, 200 mg/kg, were administered orally (os) once daily via a gastric tube, starting one week prior to the first Taxol administration and continuing for another 5 weeks (6 weeks in all).
  • The following method was used: the animals were anaesthetised with a gaseous mixture composed of 0.45 halothane, nitrogen protoxide and oxygen. Nerve conduction velocity was measured in the tail, by placing stimulating electrodes at the base of the tail and a couple of recording ring electrodes distally in the tail 5 and 10 cm with respect to the stimulating electrodes.
  • The latencies of the potentials recorded at the two sites after nerve stimulation were determined (peak to peak) and the nerve conduction velocity was calculated accordingly.
  • The site of stimulations and recording were kept fixed on repeated examinations performed in each animal by marking the tail with permanent ink.
  • The recordings and stimulations of the tail nerve were done using an Ote Biomedica Phasis II electromyograph using a stimulation intensity equal to the threshold value with a duration of 100 microseconds. In view of reports in the literature of nerve conduction velocity depending on the animal body temperature, it was necessary to keep the latter constant throughout the experiment, measuring it with a rectal probe, with the aid of a BM 70002-type thermoregulator for animals (Biomedica Mangoni).
  • The velocity was measured in all groups of animals both in basal conditions and after 5 weeks of treatment.
  • The results were expressed as means±standard deviation; significance was assessed using the “t”-test, for both independent and paired data, with a statistical significance cut-off of p<0.05.
  • The sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 2 here below.
  • TABLE 2
    Taxol-induced neuropathy: sensory nerve conduction velocity (m/s)
    measured on the animals' tails in basal conditions and after
    treatment with acetyl L-carnitine.
    MEASUREMENTS % vs % vs
    TREATMENT BASAL 5 WEEKS CONTROL TAXOL
    Control 29.5 ± 0.8 41.0 ± 0.8Δ
    (6) (6)
    Sham 30.0 ± 0.8 41.0 ± 0.3Δ
    (8) (6)
    Taxol 30.2 ± 0.9 34.0 ± 1.1**Δ −17 ± 0.03
    16 mg/kg (6) (6)
    Acetyl 30.0 ± 1.4 37.1 ± 1.8**Δσ 9 ± 0.02
    L-carnitine + (6) (6)
    Taxol 16 mg
    Taxol 30.0 ± 0.7 37.5 ± 0.4**Δ  −9 ± 0.04
    8 mg/kg (8) (8)
    Acetyl 30.2 ± 1.2 39.4 ± 0.6**Δσ 5 ± 0.02
    L-carnitine + (8) (8)
    Taxol 8 mg
    Values are means ± standard deviation.
    In brackets the number of animals used.
    t-test (independent data)
    **= p < 0.01 vs CONTR.;
    σ= P < 0.01 vs corresponding Taxol.
    t-test (paired data)
    Δ= p < 0.01 vs basal.
  • In basal conditions, all the groups of animals present well-matched nerve conduction values.
  • The measurement at 5 weeks reveals a statistically significant increase (p<0.01) in sensory nerve conduction velocity in all groups compared to basal conditions.
  • Treatment with the Taxol solvent (Sham group) did not modify nerve conduction velocity values as compared to the control group. The administration of Taxol induced a significant reduction (P<0.01) in sensory nerve conduction velocity compared to the control group; this reduction was dose-dependent: −17% with the 16 mg/kg dose and −9% with the 8 mg/kg dose.
  • Treatment with acetyl L-carnitine induced a statistically significant increase (p<0.01) in sensory nerve conduction velocity in both groups; 9% compared to the 16 mg/kg Taxol group and 5% compared to the 8 mg/kg Taxol group.
  • On the basis of the results obtained it will be noted that acetyl L-carnitine is capable of affording statistically significant protection against Taxol-induced neurotoxicity.
  • EXAMPLE 3 Protective Effect of Acetyl L-Carnitine on Taxol-Induced Weight Loss
  • The animals used in the preceding experimental model were weighed prior to starting treatment (basal values) and at the end of treatment.
  • The data given in Table 3 here below demonstrate the substantial and unexpected protective effect exerted by acetyl L-carnitine on loss of body weight caused by Taxol 16 mg/kg treatment.
  • TABLE 3
    Body weight of animals treated with Taxol alone or
    in combination with acetyl L-carnitine.
    MEASUREMENTS %
    TREATMENT BASAL 5 WEEKS % vs BASAL vs SHAM
    Control 209 ± 9.1 250 ± 12.4 +20***
    (6) (6)
    Sham  205 ± 12.2 234 ± 12.8 +14***
    (8) (8)
    Taxol 210 ± 9.1 237 ± 20.8 +13**  +1
    8 mg/kg (8) (8)
    Taxol  212 ± 12.5 180 ± 16.0 −15**  −23•
    16 mg/kg (6) (6)
    ALC + Taxol 207 ± 6.7 228 ± 20.5 +10*  −2
    8 mg/kg (8) (8)
    ALC + Taxol 210 ± 6.5 216 ± 37.6 +3  −8
    16 mg (6) (6)
    Values are means ± standard deviation.
    In brackets the number of animals used.
    t-test (independent data)
    •= p < 0.001 vs sham.
    t-test (paired data)
    *p < 0.05;
    **p < 0.01;
    ***p < 0.001 vs basal.
  • EXAMPLE 4 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Taxol-Induced Peripheral Neuropathy—Therapeutic Treatment
  • The purpose of this study is to demonstrate and evaluate the protective properties, by way of treatment, of acetyl L-carnitine.
  • Peripheral neuropathy was induced by two Taxol cycles (2.7 mg/kg/1.5 ml once a day for 5 days), 6 days apart.
  • Acetyl L-carnitine was administered subcutaneously, during the follow-up period (recovery). The dose of 100 mg/Kg/day was administered on the basis of individual body weight. Sham (NaCl 0.9%), Vehicle (Cremophor®-ethanol 1:1) and Taxol-treated animals were treated with the vehicle.
  • Nerve conduction velocity (NCV) was measured in each animal prior to start of treatment (basal), two days after the last administration of Taxol (Taxol) and weekly thereafter (recovery).
  • NCV was determined as in Example 2.
  • After the last NCV recording, the animals were sacrificed under anaesthesia and plasma was collected to measure NGF levels, according to the method by Pisano C. et al Sixth Annual Meeting (15th-18 Nov. 2001, Washington) World Federation of Neuro Oncology Societies.
  • The sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 4 here below.
  • TABLE 4
    Taxol-induced neuropathy: sensory nerve conduction velocity (m/s)
    measured on the animals' tails in basal conditions and after treatment
    with acetyl L-carnitine.
    TREATMENT RECOVERY
    2nd Taxol 3rd
    GROUP BASAL Cycle 1st week 2nd week week
    Sham 30.15 ± 3.40 32.88 ± 2.60 34.18 ± 1.62 35.66 ± 1.55 36.66 ± 1.29
    Vehicle 30.60 ± 2.41 30.64 ± 1.56 30.60 ± 1.40** 33.27 ± 1.90* 35.36 ± 2.61
    Taxol 29.30 ± 1.22 27.46 ± 2.02**§ 27.54 ± 2.66**§ 28.78 ± 2.47**§§ 30.58 ± 1.98**§§
    Acetyl L- 29.66 ± 0.74 28.24 ± 1.66**§ 30.24 ± 1.69** 33.02 ± 1.42*# 35.50 ± 2.09##
    carnitine +
    Taxol
    Values are means ± standard deviation.
    t-test (independent data)
    *= p < 0.05 vs Sham
    **= p < 0.01 vs Sham.;
    §= P < 0.05 vs Vehicle.
    §§= P < 0.01 vs Vehicle
    #= P < 0.05 vs Taxol.
    ##= P < 0.01 vs Taxol
  • NGF plasma concentrations are given in the Table 5 here below.
  • TABLE 5
    NGF plasma concentrations (ng/ml) at the end of plasma recovery
    GROUP NGF
    Vehicle 2.84 ± 0.72
    Taxol 0.19 ± 0.09§§
    Acetyl L-carnitine + Taxol 1.00 ± 0.77
    t-test (independent data)
    §§= P < 0.01 vs Vehicle
  • EXAMPLE 5 Clinical Study
  • Patients were evaluated for neurotoxicity according to WHO criteria. Patients with Peripheral Sensory Neuropathy (PSN) were treated with ALC 1 g/day i.v. infusion over 1-2 hours for at least 10 days. Twenty outpatients, 11 male and 9 females were enrolled. Patients received at least one Cisplatin- (n=4) Taxol- (n=8) based regimen or combination of both (n=8). All patients were evaluable for response having completed at least ten days of ALC therapy (median: 15 days; range 10-30). Sixteen of 20 (80%) patients showed at least one grade improvement in their PNS, while four further patients reported stable grading. No adverse events related to ALC were reported.
  • The results are shown in Table 6 below.
  • TABLE 6
    PSN (=20) Grade 0 Grade 1 Grade 2 Grade 3
    Baseline 0 3 16 1
    End treatment 2 15 3 0
  • EXAMPLE 6 Protective Effect of Acetyl L-Carnitine on Taxol-Induced Neutropenia
  • It has been ascertained by the inventors of the invention described herein that the neutropenia effect of Taxol reaches a nadir after the 3rd-4th injection.
  • To evaluate the action of ALC in combination with Taxol, both on the quantity of circulating neutrophils and on tumour growth, the degree of ALC-induced protection both on treatment with Taxol alone and in animals inoculated with a murine cancer of the breast (L-MM3) was assessed, and tumour growth was evaluated in animals treated with ALC and Taxol or with ALC alone. Taxol treatment caused a significant reduction in polymorphonucleates. Oral administration of acetyl L-carnitine combined with Taxol treatment proved capable of significantly preventing the reduction in neutrophil granulocytes induced by the anticancer agent. As regards tumour growth, Taxol, when injected according to the same schedule used for the evaluation of neutrophil granulocytes, was found to significantly inhibit the growth of L-MM3, which was monitored until the tumour reached approximately 2 cm in size. Combined treatment with Taxol and ALC for 14 days did not affect the anticancer action of Taxol.
  • In conclusion, in this tumour model, too, Taxol caused severe neutropenia and ALC, administered continuously over the period in which this type of bone marrow toxicity occurs, was capable of preventing the Taxol-induced reduction in polymorphonucleates. At the same time, the action of ALC did not affect the anticancer activity of Taxol.
  • EXAMPLE 7 Effect of Administration of Acetyl L-Carnitine (ALC) in Combination with Taxol i.p. on Neutropenia in the Mouse
  • In a study conducted according to Good Laboratory Practice (GLP), to evaluate the action of acetyl L-carnitine (ALC) in combination with Taxol on Taxol-induced neutropenia, the animals were treated both with ALC plus Taxol, and with Taxol or ALC alone. In this model, the amount of circulating neutrophils was measured.
  • Taxol was found to induce a significant reduction in neutrophil granulocytes within only 6 hours of the third injection, and thus severe neutropenia occurs with a nadir after the 3rd-4th injection.
  • Acetyl L-carnitine inner salt (sterile ampoule), solubilised in water for injectable preparations, was used. Each ampoule of ALC is dissolved in 4 ml of water for injections (Solution O). Two ml of (O) are brought up to 25 ml with sterile PBS (Sigma), so as to have 10 mg/ml for subcutaneous administration (100 mg/kg/10 ml); 0.8 ml of (O) are brought to 50 ml with PBS in order to have 2 mg/ml for oral administration (100 mg/kg/50 ml).
  • Taxol (paclitaxel (INDENA)) is weighed, solubilised in the previously prepared specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C. sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • The essential experimental indications are given here below:
  • Animals: female BALB/c mice weighing 18-20 g (Harlan).
  • Animal house conditions: 4-5 mice per cage; temperature 22±2° C.; relative humidity 55±15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7×20.7×14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • Diet: 4RF21 feed (company: Mucedola), food and water available “ad libitum”.
  • The randomisation is casual in blocks of animals, i.e. the animal house attendant transfers the mice from the boxes to the cages, completing one cage at a time. In a second phase, the attendant provisionally identifies all the mice, weighs them, and, if the weights present significant differences between groups, moves the animals from one cage to another, keeping the number of animals per cage unchanged, so as to have well-matched overall weights between one cage and another. Each cage is labelled with a card bearing the number of the group, the type of treatment (substance and/or substances injected, dose, administration route). Each animal is identified with a number from 1 to 5, written on the tail in indelible ink.
  • Animal weight: the mice are weighed prior to the start of treatment, and on day 5 or 7 and day 11.
  • The animals were treated from day 1 to day 10 with the molecules; Taxol or the vehicle were administered on alternate days, on days 5, 7, 9 and 11.
  • The groups are: 1) blanks; 2) vehicle+PBS; 3) Taxol; 4) Taxol+ALC; 5) ALC; 6) ALC+vehicle. The animals are sacrificed 6 hours after the last Taxol injection.
  • Blood and bone marrow samples are taken 6 hours after the last treatment with Taxol or vehicle. The mice are anaesthetised with CO2; blood is taken from the retro-orbital plexus (0.5 ml blood/mouse) and placed in Eppendorf test tubes containing 10 μl of Vister heparin (5000 U/ml). The animals are sacrificed by cervical dislocation. Later, the bone marrow samples are taken.
  • One blood sample and one bone marrow sample per mouse are taken at various times.
  • Blood Cell Count
  • Before starting the WBC (White Blood Cells) count, the instrument is checked by measuring the EMACHECK blood sample parameters (human check) supplied by Delcon.
  • The instrument is used according to the instructions provided in the operating manual. The blood (25 μl) is taken from the dilutor and brought to a volume of 10 ml with isotonic solution (PLTA Saline, Delcon) in a beaker (dil. 1:400) (Solution A). From Solution A, the dilutor takes 100 μl and brings them to 10 ml (dil. 1:100) in another beaker (Solution B). To Solution A are added 3 drops of lysing agent (Emosol, Delcon), the solution is mixed by hand and left to act for approximately 2 minutes so that the red blood cells are lysed and HGB (haemoglobin) released. Solution A containing the lysing agent is used for the WBC and haemoglobin (HGB) readings. Solution B is used for the RBC and platelet (PLT) readings.
  • Double readings are done on each sample, and between one sample and the next, the instrument is washed using isotonic solution,
  • Superfrost plus slides (25×75×1 mm) (Mensel-Glaser), ready for use, are adopted. The blood (8 μl) is deposited on the right-hand side of the slide; another slide, placed at a 45° angle, to the left of the blood, is drawn back until in comes into contact with the drop which spreads rapidly along the contact line between the two slides; the slide is moved forwards with a smooth, rapid movement so as to obtain a thin film of blood. The slide is left to dry in air, stained with Diff-Quick (DADE) dye, according to the attached instructions, and dried again in air.
  • The slides are immersed in Histolemon solution (Carlo Erba) for 2 sec.; a drop of synthetic mountant (Shandon) is deposited at the centre of the slide and a cover slide is placed over it covering the entire blood smear, taking care not to form bubbles between the two slides. The slides are dried and then the WBC count is performed, up to 100, with an optical microscope, after depositing a drop of cedar oil on the slide.
  • The quantity of WBC/ml, assessed using the haemocytometer, is multiplied by the percentage value of the corresponding neutrophil granulocytes of the leukocyte formula. This parameter, divided by 100, expresses the value of neutrophils/mm3 blood.
  • The following are regarded as normal parameter values: for WBC, counted on the haemocytometer, values of up to 18,000/mm3; for percentage neutrophils, counted on the slide, values of up to 18%; for absolute neutrophils calculated, values of up to 1,800.
  • Data are expressed as means±SE. Comparison between the neutrophil granulocyte values obtained for the different groups is done using ANOVA. Abnormal values are subjected to the Dixon test.
  • Taxol treatment (30 mg/kg i.p. every 2 days for a total of 4 times) caused significant neutropenia 6 hours after the last injection of the agent (−90% neutrophilic granulocytes compared to blanks, p<0.001). The oral or subcutaneous administration of acetyl L-carnitine (100 mg/kg) was found to protect the polymorphonucleates against Taxol-induced damage 8-43% sc; −23% os) (Table 7).
  • In another experiment, the combination of ALC+Taxol caused a 73% reduction in neutrophils, as against the 98% reduction obtained after administration of Taxol alone. The administration of ALC or of vehicle+ALC caused no changes in neutrophils as compared to the vehicle alone or to untreated animals (blanks) (Table 7).
  • Three days after the last administration of Taxol, neutrophil granulocytes began to recover (−64% vs vehicle), but the effect is even more marked following combined treatment with ALC+Taxol (−26% vs vehicle). In this case, too, the administration of ALC or of vehicle+ALC caused no changes in neutrophils as compared to vehicle alone or untreated animals (blanks) (Table 8).
  • TABLE 7
    Effect of ALC on Taxol-induced neutropenia in BALB/c mice
    SACRIFICE 6 HOURS AFTER LAST TAXOL ADMINISTRATION
    WBC Absolute
    Group N mm3 Lymphocytes % Neutrophils % neutrophils/mm3
    Blanks 9 5389 ± 362 88.8 ± 1.0 11.1 ± 1.0  574 ± 37
    Vehicle + PBS 7 7107 ± 743 88.8 ± 0.9 11.1 ± 0.9  769 ± 83
    Taxol 8 6781 ± 474 99.7 ± 0.1 0.2 ± 0.1 ****13 ± 8.7  
    Taxol + ALC 10 4700 ± 443 95.2 ± 0.6 4.7 ± 0.6 ***211° ± 29  
    ALC 10 6452 ± 270 88.7 ± 0.8 11.2 ± 0.7  708 ± 32
    Vehicle + ALC 10 5950 ± 551 91.5 ± 1.1 8.5 ± 1.1 511 ± 92
    Data are means ± SE.
    ****P < 0.0001 vs vehicle;
    °°P < 0.05 vs Taxol (ANOVA).
  • TABLE 8
    Effect of ALC on Taxol-induced neutropenia in BALB/c mice
    SACRIFICE 3 DAYS AFTER LAST TAXOL ADMINISTRATION
    WBC Absolute
    Group N mm3 Lymphocytes % Neutrophils % neutrophils/mm3
    Vehicle + PBS 10 4945 ± 284 85.9 ± 0.9 14.1 ± 0.9 688 ± 47
    Taxol 10 5855 ± 520 95.5 ± 0.5  4.5 ± 0.5 ****250 ± 22  
    Taxol + ALC 9 5661 ± 254 90.7 ± 0.9  9.3 ± 0.9 **511°°° ± 51  
    ALC 10 6920 ± 368 89.9 ± 0.7 10.1 ± 0.7 691 ± 50
    Vehicle + ALC 9 6067 ± 446 89.2 ± 0.9 10.7 ± 0.9 625 ± 29
    Data are means ± S.E.
    ****P < 0.0001;
    **P < 0.01 vs vehicle;
    °°°P < 0.01 vs Taxol (ANOVA).
  • Taxol, administered 4 times on alternate days, induces severe neutropenia. The oral administration of ALC is capable of affording significant protection against the damaging effect of Taxol.
  • EXAMPLE 8 Effect of Administration of Acetyl L-Carnitine (ALC) in Combination with Taxol i.v. on Neutropenia in the Mouse
  • Essentially, example 7 was repeated, except for the Taxol administration route, which in this case was intravenous, that is to say in the actual clinical application conditions.
  • The experimental schedules and measurements were the same as those described in the previous example.
  • The results are given in Tables 9 and 10.
  • TABLE 9
    Effect of ALC on Taxol-induced neutropenia in BALB/c mice
    SACRIFICE 6 HOURS AFTER FOURTH TAXOL ADMINISTRATION
    WBC Absolute
    Group N mm3 Lymphocytes % Neutrophils % neutrophils/mm3
    Vehicle 10 6400 ± 701 92.7 ± 0.5 7.3 ± 0.5 471 ± 69
    Taxol + ALC 8 6769 ± 497 88.4 ± 0.8 °°°°11.6 ± 0.8   770°°°° ± 45  
    Taxol 10 8150 ± 683 97.1 ± 0.4 ****2.9 ± 0.4   229** ± 34 
    Data are means ± SE.
    ****P < 0.0001 and P < 0.01 vs vehicle;
    °°°°P < 0.0001 vs Taxol (ANOVA).
  • TABLE 10
    Effect of ALC on Taxol-induced neutropenia in BALB/c mice
    SACRIFICE AFTER 3 DAYS' RECOVERY FROM
    LAST TAXOL ADMINISTRATION (54 mg/kg/15 ml)
    WBC Absolute
    Group N mm3 Lymphocytes % Neutrophils % neutrophils/mm3
    Vehicle 9 5817 ± 512 91.6 ± 0.9 8.4 ± 0.9 477 ± 69
    Taxol + ALC 9 5089 ± 480 92.0 ± 1.2 8.0 ± 1.2 431 ± 90
    Taxol 9 4572 ± 290 93.0 ± 1.1 7.0 ± 1.1 330 ± 57
    Blanks 8 9688 ± 497 92.1 ± 0.4 7.9 ± 0.4 758 ± 43
    Data are means ± SE.
    ****P < 0.0001 and P < 0.01 vs vehicle;
    °°°P < 0.0001 vs Taxol (ANOVA).
  • These results confirm the protective effect of orally administered ALC.
  • EXAMPLE 9 Effect of Administration of Acetyl L-Carnitine (ALC) in Combination with Taxol on Neutropenia in Mice with L-MM3 Carcinoma of the Breast and Evaluation of Anticancer Action
  • In a study conducted according to Good Laboratory Practice (GLP), to evaluate the action of acetyl L-carnitine (ALC) in combination with Taxol on tumour growth, Balb/c mice were inoculated with murine cancer of the breast (L-MM3) and the animals were treated both with ALC plus Taxol, and with Taxol or ALC alone. In addition, in this tumour model, the amount of circulating neutrophils was measured.
  • Acetyl L-carnitine inner salt (sterile ampoule), solubilised in water for injectable preparations, was used. Each ampoule of ALC is dissolved in 4 ml of water for injections (Solution O). 0.8 ml of (O) are brought to 50 ml with sterile PBS (Sigma) in order to perform the oral administration (100 mg/kg/50 ml).
  • Taxol (paclitaxel (INDENA)) is weighed, solubilised in the previously prepared specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C. sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • The essential experimental indications are given here below:
  • Animals: 120 female BALB/c mice weighing 18-20 g (Harlan)
  • Animal house conditions: 5 mice per cage; temperature 22±2° C.; relative humidity 55±15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7×20.7×14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • Diet: 4RF21 feed (company: Mucedola), food and water available “ad libitum”.
  • The randomisation is casual in blocks of animals, i.e. the animal house attendant transfers the mice from the boxes to the cages, completing one cage at a time. In a second phase, the attendant provisionally identifies all the mice, weighs them, and, if the weights present significant differences between groups, moves the animals from one cage to another, keeping the number of animals per cage unchanged. Each cage is labelled with a card bearing the number of the group, the type of treatment (substance and/or substances injected, dose, administration route). Each animal is identified with a number from 1 to 5, written on the tail in indelible ink.
  • Animal weight: the mice are weighed prior to the start of treatment, and on the day of the last Taxol injection.
  • Treatment schedule 1: the transplantable murine breast cancer cells (L-MM3) of Balb/c origin are grown at 37° C. in plastic flasks in a 5% CO2 humidified incubator in DMEM medium containing 5% heat-inactivated FCS, L-glutamine 2 mM, and gentamicin 80 μg/ml. At the time of inoculation, the cells are detached with trypsin-EDTA and re-suspended in the same medium without PBS.
  • Unanaesthetised female Balb/c mice received subcutaneous injections in the flank of 4×105 cells in 0.2 ml of DMEM.
  • Four days after inoculation of the tumour, the animals were treated with the molecules according to the following schedule, for the purposes of evaluating neutropenia:
  • Day 1 Day 4 Day 8 Day 10 Day 12 Day 14
    tumour Tax Tax Tax Tax
    inoculation ALC→→→→→→→→→→→→→→→→→→→→→
    Sacrifice
    after 6 h
  • ALC is administered then for 10 days; the animals are sacrificed after 6 hours.
  • The experimental groups, each consisting of 15 mice, are:
  • Group Cage No.
    Tumour + Vehicle 1, 2, 3
    Tumour + Taxol + ALC 4, 5, 6
    Tumour + Taxol 7, 8, 9
    Tumour + Vehicle + ALC 10, 11, 12
  • Treatment schedule 2: the same experimental groups mentioned above, each consisting of 15 mice (with different cage numbers) are treated as follows in order to evaluate survival and tumour size.
  • Day 1 Day 4 Day 8 Day 10 Day 12 Day 14 Day 17
    tumour Tax Tax Tax Tax
    inoculation ALC→→→→→→→→→→→→→→→→→→→→→→→→→
  • ALC administration lasts 14 days (in groups 16, 17, 18) and the tumour is measured until it reaches a size of approximately 2 cm. The animals are left alive for 100 days.
  • The experimental groups, each consisting of 15 mice, are:
  • Group Cage No
    Tumour + Vehicle 13, 14, 15
    Tumour + Taxol + ALC 16, 17, 18
    Tumour + Taxol 19, 20, 21
    Tumour + Vehicle + ALC 22, 23, 24
  • Table of treatments and sacrifices
  • Day Cage
    1 Inoculation: 1, 4, 7, 10, 13, 16, 19, 22
    2 Inoculation: 2, 5, 8, 11, 14, 17, 20, 23
    3 Inoculation: 3, 6, 9, 12, 15, 18, 21, 24
    4 ALC: 4, 16, 10, 22
    5 ALC: 4, 5, 16, 17, 10, 22, 11, 23
    6 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    7 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    8 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 1, 13, 10, 22
    Taxol: 4, 7, 16, 19
    9 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 2, 14, 11, 23
    Taxol: 5, 8, 17, 20
    10 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 1, 3, 13, 15, 10, 22, 12, 24
    Taxol: 4, 6, 7, 9, 16, 18, 19, 21
    11 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 2, 14, 11, 23
    Taxol: 5, 8, 17, 20
    12 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 1, 3, 13, 15, 10, 22, 12, 24
    Taxol: 4, 6, 7, 9, 16, 18, 19, 21
    13 ALC: 4, 5, 6, 16, 17, 18, 10, 22, 11,
    23, 12, 24
    Vehicle: 2, 14, 11, 23
    Taxol: 5, 8, 17, 20
    14 ALC: 5, 6, 17, 18, 11, 23, 12, 24, 16,
    22
    Vehicle: 1, 3, 13, 15, 10, 22, 12, 24
    Taxol: 4, 6, 7, 9, 16, 18, 19, 21
    Sacrifice 6 h after last Taxol
    administration in:
    1, 4, 7, 10 + 3 blank animals
    15 ALC: 6, 18, 12, 24, 16, 22, 17, 23
    Vehicle: 2, 14, 11, 23
    Taxol: 5, 8, 17, 20
    Sacrifice 6 h after last Taxol
    injection in:
    2, 5, 8, 11 + 3 blank animals
    16 ALC: 16, 22, 17, 23, 18, 24
    Taxol: 6, 9, 18, 21
    Vehicle: 3, 15, 12, 24
    Sacrifice 6 h after last Taxol
    injection in:
    3, 6, 9, 12 + 4 blank animals
    17 ALC: 16, 22, 17, 23, 18, 24
    18 ALC: 17, 23, 18, 24
    19 ALC: 18, 24
  • The tumour size (length and width) is measured twice a week with a calliper, from the time the tumour is measurable. Tumour size is expressed in cm and is evaluated according to the following formula: √(length×width).
  • Tumour measurement schedule
    Day after inoculation Cage (comprising 5 mice)
    22 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    23 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    28 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    30 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    35 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    36 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    42 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    44 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
    49 13, 16, 19, 22
    14, 17, 20, 23
    15, 18, 21, 24
  • Blood and bone marrow samples are taken for the first treatment schedule animals. On the days they are sacrificed, the mice are anaesthetised with CO2; blood is taken from the retro-orbital plexus (0.5 ml blood/mouse) and placed in Eppendorf test tubes containing 10 μl of Vister heparin (5000 U/ml). The animals are sacrificed by cervical dislocation. Later, the bone marrow samples are taken.
  • One blood sample and one bone marrow sample per mouse are taken at various times.
  • Blood Cell Count
  • Before starting the WBC count, the instrument is checked by measuring the EMACHECK blood sample parameters (human check) supplied by Delcon.
  • The instrument is used according to the instructions provided in the operating manual. The blood (25 μl) is taken from the dilutor and brought to a volume of 10 ml with isotonic solution (PLTA Saline, Delcon) in a beaker (dil. 1:400) (Solution A). From Solution A, the dilutor takes 100 μl and brings them to 10 ml (dil. 1:100) in another beaker (Solution B). To Solution A are added 3 drops of lysing agent (Emosol, Delcon), the solution is mixed by hand and left to act for approximately 2 minutes so that the red blood cells are lysed and HGB (haemoglobin) released. Solution A containing the lysing agent is used for the WBC and haemoglobin (HGB) readings. Solution B is used for the RBC and platelet (PLT) readings.
  • Double readings are done on each sample and between one sample and the next, the instrument is washed using isotonic solution,
  • Superfrost plus slides (25×75×1 mm) (Mensel-Glaser), ready for use, are adopted. The blood (8 μl) is deposited on the right-hand side of the slide; another slide, placed at a 45° angle, to the left of the blood, is drawn back until in comes into contact with the drop which spreads rapidly along the contact line between the two slides; the slide is moved forwards with a smooth, rapid movement so as to obtain a thin film of blood. The slide is left to dry in air, stained with Diff-Quick (DADE) dye, according to the attached instructions, and dried again in air.
  • The slides are immersed in Histolemon solution (Carlo Erba) for 2 sec; a drop of synthetic mountant (Shandon) is deposited at the centre of the slide and a cover slide is placed over it covering the entire blood smear, taking care not to form bubbles between the two slides. The slides are dried and then the WBC count is performed, up to 100, with an optical microscope, after depositing a drop of cedar oil on the slide.
  • The quantity of WBC/ml, assessed using the haemocytometer, is multiplied by the percentage value of the corresponding neutrophil granulocytes of the leukocyte formula. This parameter, divided by 100, expresses the value of neutrophils/mm3 blood.
  • Comparison between the neutrophil granulocyte values obtained for the different groups is done using ANOVA. Tumour sizes are compared using the non-parametric Mann Whitney test for unpaired data.
  • Taxol treatment (30 mg/kg i.p. every 2 days for a total of 4 times) caused a significant reduction in polymorphonucleates (−93%, vs vehicle, p<0.0001) in the mouse inoculated with L-MM3 murine breast cancer. The oral administration of acetyl L-carnitine (100 mg/kg once daily for 10 days) combined with treatment with Taxol proved capable of significantly counteracting the Taxol-induced reduction in neutrophil granulocytes (335/mm3 vs 65/mm3, oop<0.01) (Table 11).
  • Taxol, injected according to the same schedule used for evaluation of neutrophil granulocytes (30 mg/kg i.p. every two days for a total of 4 times), was found to significantly inhibit L-MM3 tumour growth, which was monitored until the tumour size reached approximately 2 cm in the control group (0.56 cm vs 1.8 cm, p<0.0001). Combined treatment with orally administered ALC for 14 days (100 mg/kg once daily) plus Taxol (30 mg/kg i.p. every 2 days for a total of 4 times) did not affect the anticancer activity of Taxol.
  • In conclusion, in this tumour model, too, Taxol caused severe neutropenia and ALC, administered continuously over the period in which this type of bone marrow toxicity occurs, was capable of preventing the Taxol-induced reduction in polymorphonucleates. At the same time, the action of ALC did not affect the anticancer activity of Taxol.
  • A possible mechanism of reduction of Taxol toxicity by ALC was given in Pisano C. et al., XII National Congress of Italian Toxicology Society, Bologna, 23-26 Feb., 2000 and 91st Annual Meeting of American Association for Cancer Research, San Francisco, Apr. 1-6, 2000, see Proceedings, Abst. No 3867
  • TABLE 11
    Effect of ALC on Taxol-induced neutropenia in BALB/c mice inoculated with murine carcinoma
    of the breast (L-MM3)
    SACRIFICE 6 HOURS AFTER LAST TAXOL ADMINISTRATION
    WBC Absolute Platelets
    Group N mm3 Lymphocytes % Neutrophils % neutrophils/mm3 mm3
    Vehicle 10 7600 ± 729 86.8 ± 1.1 13.2 ± 1.1   965 ± 110 402600 ± 22727
    Tax + ALC 15 5820 ± 250   94 ± 0.8 ****5.9°°° ± 0.8    ****335°° ± 45   502733 ± 27474
    Taxol 13 4600 ± 242 98.6 ± 0.2 ****1.3 ± 0.2   ****65 ± 14 539000 ± 36057
    Vehicle + 12 4950 ± 385 87.6 ± 0.9 12.3 ± 0.9 ***607 ± 68 433000 ± 45731
    ALC
    Data are means ± SE.
    ****P < 0.0001;
    ***P < 0.001 vs vehicle;
    °°P < 0.01;
    °°°P < 0.001 vs Taxol (ANOVA).
  • EXAMPLE 10 Evaluation of the Effect of Acetyl L-Carnitine (ALC) on the Anticancer Action of Taxol in M109 Murine Lung Cancer
  • Taxol has proved effective in the treatment of cancer of the ovaries, breast and lungs and in other types of cancers (Rowinsky, E. K., and R. C. Donehower, (1991); Pharmacol. Ther. 52:35). The anticancer action of this compound is related mainly to its ability to inhibit depolymerisation of the microtubules in tubulin monomers (Schiff, P. B., J. Fant, and S. B. Horwitz. 1979. Nature); this effect blocks the proliferating cells in the G2/M phase of the cell cycle, i.e. between the last stage of the interphase in which DNA synthesis is completed and the subsequent period of cell division or mitosis, and this leads, in the cell, to the start of a cascade of events typical of the apoptotic process. Taxol, like other chemotherapeutic agents, moreover, is associated with side effects such as neuropathies and myelosuppression.
  • On comparing the statistical data obtained in the groups of animals treated with vehicle alone and those treated with Taxol in combination with orally administered acetyl L-carnitine, a statistically significant reduction of the tumour mass was found in the latter at all observation times. By contrast, comparison of the group treated with vehicle alone and the one treated with vehicle in combination with administration of acetyl L-carnitine revealed no statistically significant differences in tumour mass growth at any of the observation times. Analysis of the data relating to the comparison between the group treated with Taxol and the one treated with Taxol in combination with acetyl L-carnitine showed no significant differences in tumour weight. As regards the analysis of the number of metastases, the data obtained show a statistically significant reduction in that number in the groups treated with Taxol, with Taxol in combination with acetyl L-carnitine and with vehicle in combination with acetyl L-carnitine as compared to the group treated with vehicle alone. In particular, the mice treated with Taxol or with Taxol in combination with acetyl L-carnitine also showed a reduction in the diameter of the metastases compared to the groups treated with vehicle alone or with vehicle in combination with acetyl L-carnitine. On the basis of analysis of the following data, we can therefore state that acetyl L-carnitine does not interfere with the anticancer action of Taxol in terms of inhibition of the tumour mass. In addition, acetyl L-carnitine showed a significant inhibitory effect on the formation of lung metastases.
  • The following example illustrates this further aspect of the invention.
  • EXAMPLE 11 Evaluation of the Effect of Acetyl L-Carnitine (ALC) on the Anticancer Action of Taxol in Mice with M109 Lung Cancer
  • In a study conducted according to Good Laboratory Practice (GLP), to evaluate the action of acetyl L-carnitine (ALC) in combination with Taxol on tumour growth, Balb/c mice were inoculated with murine lung cancer (M109) and the animals were treated both with ALC plus Taxol, and with Taxol or ALC alone. In addition, in this tumour model, the amount of circulating neutrophils was measured.
  • Acetyl L-carnitine inner salt (sterile ampoule, 0.5 g), solubilised in water for injectable preparations, was used.
  • Each ampoule of ALC is dissolved in 4 ml of the solvent provided (solution O). To be precise, 1.6 ml of solution (O) are brought to 40 ml with sterile buffer solution (PBS, Sigma P-4417) and then administered orally (100 mg/kg/20 ml).
  • Taxol (paclitaxel (INDENA), cod. 3926570) is weighed, solubilised in the specific vehicle (Cremophor® EL (BASF), diluted 1:1 with ethanol) and stored at +4° C., sheltered from the light. At the time of use, the 12 mg/ml solution is diluted 1:4 with saline in phosphate buffer (PBS) (SIGMA) and injected i.p. (30 mg/kg/10 ml).
  • Animals: 60 female Balb/c mice weighing 18 g (Harlan).
  • Animal house conditions: 5 mice per cage; temperature 22±2° C.; relative humidity 55±15%; air changes 15-20/h; 12 h light/darkness cycle (7.00 a.m.-7.00 p.m. light); makrolon cages (26.7×20.7×14 cm) with stainless steel grilled covers; dedusted, sterile, corn-cob shaving litters.
  • Diet: 4RF21 feed (company: Mucedola), food and water available “ad libitum”.
  • Randomisation: casual in blocks of animals.
  • Animal weight: the mice are weighed prior to the start of treatment and then once a week up to the end of the experiment.
  • M109 tumour cells isolated from solid tumour.
  • The procedure described by Kedar E., B. Ikejiri, G. D. Bannard and R. B. Herberman (Eur. J. Cancer Clin. Oncol. 18; 991: 1982) is adopted with modifications. One Balb/c mouse (donor) was sacrificed by cervical dislocation, and after washing its back with denatured alcohol, the dorsal skin was cut longitudinally into two flaps which were detached to remove the tumour mass. The latter was placed on sterile gauze, where it was freed of connective tissue adhesions and of the necrotic and haemorrhagic parts. The study tissue was placed in a plate containing PBS with Ca++ and Mg++ (Gibco) at pH 7.2 and cold, broken down into pieces measuring 2-3 mm and re-suspended in a solution (15 ml solution/g tumour) of PBS with Ca++ and Mg++, pH 7.2 containing 2 mg/ml trypsin (type III, 10000 U/mg protein, Sigma-Aldrich), 2 mg/ml collagenase (type I-S 180 U/mg solid, Sigma), 0.2 mg/ml DNAse (type I 1548 U/mg protein, Sigma) and 25 μg/ml gentamicin (Sigma) and incubated at 37° C. for 15 minutes under constant stirring. The cell suspension was then homogenised with the aid of a potter (B. Braun) for 2 minutes, incubated at 37° C. for 10 minutes and aspirated gently a number of times with a syringe with a No. 21 sterile needle. After addition of 30 ml of RPMI-1640 (Eurobio) containing 10% FBS (Eurobio) maintained at 4° C., the cell suspension was filtered on sterile gauze and then centrifuged at 700 g for 10 minutes. The cell pellet was re-suspended gently in RPMI-1640 containing 10% FBS and 0.2 mg/ml DNAse (Sigma) and then centrifuged at 700 g for 10 minutes. The pellet was consecutively subjected to two washings with RPMI-1640; at the end of the last washing, the pellet was re-suspended gently in RPMI-1640 so as to perform the count to establish the cell concentration.
  • Cell count under the microscope: the cell count is performed by means of the Trypan-Blue vital stain exclusion test; the tumour cells are suitably diluted with 0.4% Trypan-Blue (Sigma), a vital stain, which makes it possible to distinguish between viable and dead cells. The dilution containing the cells to be counted was stirred gently, 10 μl were removed and used to set up a Burker chamber. A square grid delimited by the three triple lines was used, comprising 16 small squares (4×4) delimited from one another by double lines. Both viable cells (which have a translucid appearance) and dead cells (which are blue in appearance, having incorporated the stain) which are to be found positioned inside the square formed by the median line and the triple lines, or on the line itself. This operation was repeated for another three squares, after which the sum of the cells counted in each square was calculated and the arithmetic mean was determined for the readings taken in the four squares. The arithmetic mean of the viable cells was multiplied by the dilution factor used and by the specific power factor for the type of chamber used for the count (104), thus obtaining the number of viable cells contained in one millilitre. The ratio of the arithmetic mean of the viable cells to the arithmetic mean of total cells, multiplied by a hundred, expresses the percentage cell viability.
  • Inoculation conditions: 60 unanaesthetised Balb/c mice weighing approximately 18 g (Harlan) received i.m. injections of 3×105 M109 lung cancer cells in 0.1 ml of RPMI-1640 (Sigma) in the right rear paw.
  • Treatment schedule: for the purposes of evaluating tumour size in the experimental study groups, each consisting of 15 Balb/c mice, 3×105 M109 lung cancer cells were inoculated, and the study molecules were administered at the scheduled times. ALC was administered at the dose of 100 mg/kg (os) from day 4 to day 17. The vehicle diluted 1:4 with PBS from the mother solution was administered i.p. on days 8, 10, 12 and 14. Taxol was administered at the dose of 30 mg/kg (i.p.) on days 8, 10, 12 and 14, as described in the following schedule:
  • Day 1 Day 4 Day 8 Day 10 Day 12 Day 14 Day 17
    tumour Tax Tax Tax Tax
    inoculation ALC→→→→→→→→→→→→→→→→→→→→→→
  • The animals were kept under observation up to day 22 after inoculation of the M109 murine lung cancer cells, and were then sacrificed and the lungs removed to determine the number of metastases.
  • The experimental groups, each comprising 15 mice, are:
  • Group Cage No
    Tumour + Vehicle + ALC 1, 2, 3
    Tumour + Taxol + ALC 4, 5, 6
    Tumour + Taxol 7, 8, 9
    Tumour + Vehicle 10, 11, 12
  • Treatment Table:
  • DAY TREATMENT CAGE No
    1 Cell inoculation 1, 2, 3, 4, 5, 6, 7, 8,
    9, 10, 11, 12,
    2 ALC 1, 2, 3, 4, 5, 6
    3 ALC 1, 2, 3, 4, 5, 6
    4 ALC 1, 2, 3, 4, 5, 6
    5 ALC 1, 2, 3, 4, 5, 6
    6 ALC 1, 2, 3, 4, 5, 6
    TAX 4, 5, 6, 7, 8, 9
    VEHIC 1, 2, 3, 10, 11, 12,
    7 ALC 1, 2, 3, 4, 5, 6
    8 ALC 1, 2, 3, 4, 5, 6
    TAX 4, 5, 6, 7, 8, 9
    VEHIC 1, 2, 3, 10, 11, 12,
    9 ALC 1, 2, 3, 4, 5, 6
    10 ALC 1, 2, 3, 4, 5, 6
    TAX 4, 5, 6, 7, 8, 9
    VEHIC 1, 2, 3, 10, 11, 12,
    11 ALC 1, 2, 3, 4, 5, 6
    12 ALC 1, 2, 3, 4, 5, 6
    TAX 4, 5, 6, 7, 8, 9
    VEHIC 1, 2, 3, 10, 11, 12,
    13 ALC 1, 2, 3, 4, 5, 6
    14 ALC 1, 2, 3, 4, 5, 6
    15 ALC 1, 2, 3, 4, 5, 6
  • Tumour Measurement Schedule:
  • Day after inoc. Cage No
    4 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    8 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    11 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    13 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    15 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    18 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    20 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
  • Tumour measurement: the tumour was measured with a calliper three times a week as soon as it became palpable. The tumour mass is calculated on the basis of the measurements of the two dimensions (length and width), expressed in mm, according to the following formula:
  • ( length × width 2 ) 2 = tumour volume ( mm 3 ) .
  • If we consider conventionally a tumour density equal to 1, the result is that the tumour volume expressed in mm3 is equal to the weight of the tumour expressed in mg.
  • Determination of the number of lung metastases: on day 22 after inoculation of M109 murine lung cancer, the study animals were sacrificed by cervical dislocation. Their lungs were then removed and kept for approximately 5-7 days in 5 ml of Bouin's solution with the following composition: 71% as saturated picric acid solution (Merck), and 24% as 10% formaldehyde (Fluka). When lung metastases were evident, their numbers were counted.
  • Statistical analysis of the data for tumour size and number of lung metastases was carried out using the non-parametric Mann-Whitney test for unpaired data.
  • Analysis of the data obtained in the various groups of study animals shows inhibition of tumour mass growth in the group of mice treated with 30 mg/kg of Taxol administered i.p., as compared to the mice in the group treated with vehicle alone (Table 12). This phenomenon is already marked after only the first administration of the agent, and the difference in weight of the tumours between the group treated with Taxol and the one treated with vehicle alone proved statistically significant with p<0.01. After the second administration, the reduction in tumour mass observed is maintained, with a statistically significant difference (p<0.0001) as compared to the group treated with vehicle alone, and this inhibition of the tumour mass is maintained after the following administrations. The group of animals treated with Taxol administered i.p. at the dose of 30 mg/kg in combination with acetyl L-carnitine administered orally at the dose of 100 mg/kg already shows a reduction in tumour mass growth as compared to vehicle alone after the first treatment, with a significance of p<0.05. This trend is also maintained after the subsequent administrations. On all the days the analysis was carried out, the group treated with vehicle in combination with acetyl L-carnitine showed no highly significant difference in tumour mass growth as compared to the group treated with vehicle alone. Moreover, comparison between the group treated with Taxol alone and the one receiving combined treatment with Taxol plus acetyl L-carnitine revealed no statistically significant differences in tumour mass size on any of the days analysed. On the basis of analysis of the following data, we can therefore state that acetyl L-carnitine does not interfere with the anticancer action of Taxol in terms of inhibition of the tumour mass.
  • As regards the analysis of the number of metastases at the end of the experiment, the data obtained show a statistically significant reduction in their number in the groups treated with Taxol, with Taxol combined with acetyl L-carnitine and with vehicle combined with acetyl L-carnitine as compared to the group treated with vehicle alone. In particular, the mice treated with Taxol or with Taxol combined with acetyl L-carnitine also showed a reduction in the diameter of the metastases as compared to the groups treated with vehicle alone or with vehicle plus acetyl L-carnitine (see Table 13). On the basis of analysis of the data obtained, it might be suggested that acetyl L-carnitine has a mild inhibitory effect on the formation of lung metastases.
  • TABLE 12
    Effect of combined ALC + Taxol treatment on growth of M109 murine lung cancer in BALB/c mice
    Tumour size
    (mm3 ± SEM)
    Group Day 8 Day 11 Day 13 Day 15 Day 20
    Vehicle 563 ± 26 917 ± 59 1137 ± 50   1583 ± 62 2305 ± 146
    Vehicle + ALC 567 ± 33 959 ± 50 1289 ± 39  1779* ± 57 2251 ± 78 
    Taxol 539 ± 43 662** ± 50  622**** ± 47   857**** ± 70 1085**** ± 116  
    Taxol + AL 585 ± 25 716* ± 51  714*** ± 61  919**** ± 63 1202**** ± 72   
    Female BALB/c mice weighing approximately 20 g received i.m. injections of M109 murine lung cancer (3 × 105 cells/mouse).
    The tumours were measured on the post-inoculation days indicated. Animals were treated with ALC and Taxol according to the treatment schedule.
    Significance was assessed by comparing the various treatment groups versus the group treated with vehicle alone
    *P < 0.05,
    **P < 0.01,
    ***P < 0.001,
    ****P < 0.0001
    The data were analysed statistically using the Mann-Whitney test for paired data.
  • TABLE 13
    Determination of number of lung metastases on day 22
    after inoculation of M109 murine lung cancer in
    BALB/c mice after combined treatment with Taxol + ALC.
    Group n animals (Mean ± SD) Size of metastases
    Vehicle 6  109.3 ± 20.1 M, S
    ♦Vehicle + ALC 9  **77 ± 13.2 M, S
    ♦Taxol 12 ***9 ± 7.3 S
    ♦Taxol + ALC 13 ***5 ± 2.4 S
    Female BALB/c mice weighing approximately 20 g received i.m. injections of M109 murine lung cancer (3 × 105 cells/mouse).
    Animals were treated with ALC and Taxol according to the treatment schedule. On day 22 after inoculation of the tumour, the animals were sacrificed, their lungs were removed and stored in Bouin's solution. The number of metastases was evaluated 10 days after taking the samples.
    ♦Significance was assessed by comparing the various treatment groups versus the group treated with vehicle alone
    *P < 0.05 vs vehicle,
    **P < 0.01,
    ***P < 0.001.
    Taxol vs Taxol + ALC was significant (P < 0.05).
    The data were analysed statistically using the Mann-Whitney test for paired data.
    M = Medium (1-2 mm diam);
    S = Small (<1 mm diam).
  • ALC does not interfere with the therapeutic effect of Taxol, and this aspect has also been evaluated in a human tumour model, as illustrated in the following example.
  • EXAMPLE 12 Study of the Influence of Acetyl L-Carnitine (ALC) Treatment on the Anticancer Activity of Taxol in a Human Tumour Model
  • Cell cultures of LOVO human colon cancer, transplanted in hairless mice, were used.
  • The tumour was inoculated in solid fragments in both flanks of the mice (day 0).
  • The inoculated tumours were measured with a calliper and when a mean tumour weight of 100 mg was reached (day 7); the animals were divided into 4 groups of 5 animals each according to the following schedule:
  • Group 1 Controls
    Group 2 Taxol
    Group 3 ALC
    Group 4 Taxol + ALC
  • On the same day, ALC treatment was initiated and was continued for 18 consecutive days (qdx18) (groups 3 and 4). ALC was administered at the dose of 100 mg/kg with an administration volume of 25 ml/kg.
  • Taxol (54 mg/kg/15 ml/kg) was administered i.v. according to a schedule consisting in a total of 4 administrations at 4-day intervals (q4dx4; days 10, 14, 18, 22) (groups 2 and 4).
  • In the course of the treatment and in the following three weeks at fortnightly intervals, the tumours were measured and tumour volume inhibition was calculated (TVI %, calculated as 100−(mean weight of tumours treated/mean weight of control tumours×100)) as obtained with the various treatments.
  • Taxol treatment caused an inhibition of tumour growth (TVI=88%). Treatment with ALC had no effect on tumour growth, which was similar to that in control group tumours. Combined treatment with Taxol plus ALC showed an anticancer efficacy (TVI=90%) almost identical to that achieved with Taxol alone, confirming that ALC does not interfere with the cytotoxic activity of Taxol.
  • EXAMPLE 13 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Cisplatin-Induced Peripheral Neuropathy—Prevention and Therapeutic Treatment
  • The purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention and treatment, of acetyl L-carnitine administered in the coordinated use with Cisplatin.
  • Peripheral neuropathy was induced by intraperitoneally administration of Cisplatin (2 mg/kg ml) dissolved in distilled sterilized water, once a week for 9 weeks (for a total of 9 administrations).
  • Acetyl L-carnitine was administered orally at the dose of 100 mg/Kg/day. ALC treatment occurred daily, starting one week before Cisplatin treatment (preventive treatment) or one week after the first Cisplatin treatment (therapeutic).
  • Sensory nerve conduction velocity (SNCV) was measured by means of the H reflex, to that the tibial nerve was stimulated by using a two pair of electrodes inserted subcutaneously 3 cm distally; EMG recordings from the plantar foot muscle were made with a pair of skin clips surface electrodes.
  • The latencies of the potentials recorded after nerve stimulation between the two sites were determined (peak to peak) and the nerve conduction velocity was calculated accordingly.
  • The recordings and stimulations of the nerve were done using an Ote Biomedica Phasis II electromyograph. In view of reports in the literature of nerve conduction velocity depending on the animal body temperature, it was necessary to keep the latter constant throughout the experiment, measuring it with a rectal probe, with the aid of a BM 70002-type thermoregulator for animals (Biomedica Mangoni).
  • Sensory nerve conduction velocity measured as H reflex are given in the Table 14 here below.
  • TABLE 14
    Cisplatin-induced neuropathy: sensory conduction velocity
    (H reflex) in m/s measured in basal conditions and
    after treatment with acetyl L-carnitine.
    TREATMENT
    GROUP BASAL Cisplatin 9th treatment
    Control 63.5 ± 0.7 72.3 ± 1.0
     (6)  (6)
    Cisplatin 64.2 ± 0.9 57.8 ± 0.9**Δ
    (10) −25.0.03§
    (10)
    ALC + Cisplatin 63.8 ± 1.0 63.7 ± 2.9**▴
    (10) 9.005#
     (8)
    Cisplatin + ALC 64.2 ± 0.6 63.5 ± 2.1**▴
    (10) 9.005#
     (9)
    ALC + Cisplatin: preventive treatment
    Cisplatin + ALC: therapeutic treatment
    Values are means ± standard deviation
    (number of animals)
    §% vs Control
    #% vs Cisplatin
    t-test (independent data) **p < 0.01 vs CTR; ▴p < 0.01 vs Cisplatin
    t-test (matched data) Δp < 0.01 vs Basal
  • The nerve conduction velocity on the caudal nerve was determined as in Example 2; data measured are given in the Table 15 here below.
  • TABLE 15
    Cisplatin-induced neuropathy: sensory conduction velocity in m/s
    measured on the caudal nerve in basal conditions and
    after treatment with acetyl L-carnitine.
    TREATMENT
    GROUP BASAL Cisplatin 9th treatment
    Control 29.6 ± 0.7 41.2 ± 1.4Δ
     (6)  (6)
    Cisplatin 29.6 ± 1.0 35.7 ± 1.1**Δ
    (10) −15.0.03§
    (10)
    ALC + Cisplatin 29.5 ± 0.8 39.5 ± 0.7*Δ▴
    (10) 10.04#
     (8)
    Cisplatin + ALC 30.5 ± 0.7 38.2 ± 0.7**Δ▴
    (10) 7.004#
     (9)
    ALC + Cisplatin: preventive treatment
    Cisplatin + ALC: therapeutic treatment
    Values are means ± standard deviation.
    §% vs Control
    #% vs Cisplatin
    t-test (independent data) *p < 0.05 vs CTR; **p < 0.01 vs CTR; ▴p < 0.01 vs Cisplatin
    t-test (matched data) Δp < 0.01 vs Basal
  • EXAMPLE 14 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Oxaliplatin-Induced Peripheral Neuropathy—Prevention Treatment
  • The purpose of this study is to demonstrate and evaluate the protective properties, by way of prevention, of acetyl L-carnitine administered one day prior to Oxaliplatin treatment for 4 weeks.
  • Peripheral neuropathy was induced by intraperitoneally administration of Oxaliplatin (2.7 mg/kg/1.5 ml) dissolved in distilled sterilized water, twice a week for 4 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was dissolved in distilled water and administered subcutaneously. The dose of 100 mg/1.5 ml/Kg/day was administered on the basis of individual body weight. ALC treatment occurred daily, starting a day before Oxaliplatin treatment, for 4 weeks. Control and Oxaliplatin groups were treated s.c. with vehicle.
  • Nerve conduction velocity (NCV) was measured in each animal under halotane anaesthesia, prior to start of treatment (basal), and the day after the last administration of Oxaliplatin (final).
  • NCV was determined as in Example 2.
  • The sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 16 here below.
  • TABLE 16
    Oxaliplatin-induced neuropathy: sensory nerve conduction
    velocity (m/s) measured on the animals' tails in basal
    conditions and after treatment with acetyl L-carnitine.
    TREATMENT
    GROUP BASAL Oxaliplatin 8th treatment
    Control 31.0 ± 1.4 35.8 ± 1.7
    Oxaliplatin 31.3 ± 1.7 30.8 ± 1.2*
    Acetyl L-carnitine + Oxaliplatin 31.4 ± 1.5 33.6 ± 2.6§
    Values are means ± standard deviation.
    t-test (independent data)
    *= p < 0.001 vs Control
    §= p < 0.001 vs Oxaliplatin
  • EXAMPLE 15 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Oxaliplatin-Induced Peripheral Neuropathy—Therapeutic Treatment
  • The purpose of this study is to demonstrate and evaluate the protective properties, by way of therapy, of acetyl L-carnitine administered at the end of Oxaliplatin treatments, during a follow-up period of 3 weeks.
  • Peripheral neuropathy was induced by intraperitoneal administration of Oxaliplatin (3 mg/kg/1.5 ml) dissolved in distilled sterilized water, twice a week for 4 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was dissolved in distilled water and administered subcutaneously during the follow-up period. The dose of 100 mg/1.5 ml/Kg/day was administered on the basis of individual body weight. ALC treatment started the day after the last Oxaliplatin administration. Control and Oxaliplatin groups were treated s.c. with vehicle.
  • Nerve conduction velocity (NCV) was measured in each animal under halotane anaesthesia, prior to start of treatment (baseline), the day after the last administration of Oxaliplatin (final) and weekly after a follow-up period of 3 weeks (recovery).
  • NCV was determined as in Example 2.
  • The sensory nerve conduction velocity data measured on the caudal nerve are given in the Table 17 here below.
  • TABLE 17
    Oxaliplatin-induced neuropathy: sensory nerve conduction velocity (m/s)
    measured on the animals' tails in basal conditions and after treatment
    with acetyl L-carnitine.
    TREATMENT RECOVERY
    8th Oxaliplatin 3rd
    GROUP BASAL Treatment 1st week 2nd week week
    Control 31.0 ± 1.4 35.8 ± 1.7*** 37.8 ± 2.3*** 38.2 ± 1.5*** 39.3 ± 1.2***
    Oxaliplatin 31.6 ± 2.0 30.8 ± 0.6*** 31.6 ± 2.7*** 32.0 ± 1.9** 35.9 ± 1.8*
    Acetyl L-carnitine + 31.0 ± 1.7 30.6 ± 1.5 34.1 ± 2.6§ 35.1 ± 1.9§§ 36.6 ± 2.0
    Oxaliplatin
    Values are means ± standard deviation.
    t-test (independent data)
    *= p < 0.01 vs Control;
    **= p < 0.005 vs Control;
    ***= p < 0.0001 vs Control
    §= p < 0.05 vs Oxaliplatin;
    §§= p < 0.01 vs Oxaliplatin
  • EXAMPLE 16 Protective Effect of Acetyl L-Carnitine on Peripheral Nerve from Oxaliplatin-Induced Damage—Therapeutic and Preventive Treatment
  • The paw-withdrawal test (Randall-Sellitto) was used to address mechanical hyperalgesia. Nociceptive threshold was measured using an Ugo Basile analgesimeter by applying an increasing pressure to the left and right hind paws. The nociceptive threshold is defined as the pressure (grams) at which the rat withdraws its paw. Lower thresholds correspond to an increased sensitivity to algesia. This behavioural test is conducted at different times (days) from drug or vehicle administration. Rats are made familiar with the testing procedure and with handling by investigators during the week prior to the experiments.
  • Wistar female rats (Charles River) about 10 weeks of age were used. Oxaliplatin (3 mg/kg i.p.) in saline was administered three times a week. For the first two weeks, then two times a week for two weeks and then once a week.
  • The preventive treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily from 1st day of the Oxaliplatin treatment.
  • The therapeutic treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily from 24th day of the Oxaliplatin treatment.
  • The nociceptive threshold data (Randall-Sellitto) are given in the Table 18 here below.
  • TABLE 18
    Oxaliplatin-induced neuropathy: nociceptive threshold (g) measured on
    the animals' hind paws (mean ± S.D.)
    Days
    GROUP N 0 9 16 23 30 37 40 44 51
    Saline 9 235 245 214 204 234 215 234 204 209
    48.6  83.8d  38.4 a  41.5 d  59.5 d  37.2  65.3 d  39.4  46.0
    Saline + 9 210 244 232 234 250 260 216 199 216
    ALC 37.4  99.1 d  65.5 d  77.9 d  96.9 d 100.0 a  79.6 a  81.8  82.7
    Oxaliplatin 15 217 129 133 121 114 151 143 147 136
    41.2  35.7  41.2  42.2  59.1  94.3  46.4 107.1  60.8
    ALC + 12 242 206 207 238 247 276 241 283 291
    Oxaliplatin 34.0 104.5 110.8 a  46.0 d 115.7 d 114.0 d  87.5 d 129.4 d  95.2 d
    Preventive
    ALC + 14 221 181 136 116 182 179 191 186 196
    Oxaliplatin 32.3  92.2  44.0  42.1  76.9  50.2  46.3  68.5  98.1
    Therapeutic
    ANOVA: Dunnett's multiple comparison test vs Oxaliplatin.
    a = p < 0.05,
    d = p < 0.001.
  • The statistical analysis is given in Table 19 herein below.
  • TABLE 19
    D.F. F P<
    Saline vs Oxaliplatin (0-51 days)
    Treatment 1-22  28.16 0.001
    Interaction 8-176 2.03 0.045
    Oxaliplatin + ALC - Preventive
    Vs Oxaliplatin (0-51-days)
    Treatment 1-25  21.98 0.001
    Interaction 8-200 3.06 0.003
    Saline vs Oxaliplatin (23-51 days)
    Treatment 1-22  17.95 0.001
    Interaction 5-110 1.10 NS
    Oxaliplatin + ALC - Therapy
    Vs Oxaliplatin (23-51-days)
    Treatment 1-27  6.03 0.021
    Interaction 5-135 1.41 NS
  • EXAMPLE 17 Protective Effect of Acetyl L-Carnitine on Peripheral Nerve from Vinorelbine-Induced Damage—Therapeutic and Preventive Treatment
  • The paw-withdrawal test (Randall-Sellitto) was used as in Example 15. Sprague Dawley male rats (Harlan) about 3 months of age (about 350 g) were used. Vinorelbine (0.200 mg/kg i.p.) in saline was administered three times a week until the end of the experiment.
  • The preventive treatment was conducted as follows: Vinorelbine 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 1st day of the Vinorelbine treatment. Treatment with ALC went until day 25.
  • The therapeutic treatment was conducted as follows: Oxaliplatin 3 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 9th day of the Vinorelbine treatment. Treatment with ALC went until day 25.
  • The test was done twice a week.
  • The nociceptive threshold data (Randall-Sellitto) are given in the Table 20 here below.
  • TABLE 20
    Vinorelbine-induced neuropathy: nociceptive threshold (g) measured on
    the animals' hind paws (mean ± S.D.)
    Days
    GROUP N 0 4 9 11 16 18 23 25
    Saline 10 228.5 214.6 229.8 242.9 220.7 205.0 196.8 214.8
    44.1 59.2  54.1 c  35.5 d  38.6 d  38.9 d  46.9 c  54.5 d
    Vinorelbine 12 211.4 173.5 168.8 156.8 135.8 127.7 136.2 122.7
    32.9 51.6  45.6  50.5  36.0  38.2  45.4  40.9
    ALC + 10 222.0 197.4 234.0 211.2 235.4 204.6 202.6 201.2
    Vinorelbine 23.8 39.6  74.5 a  41.4 b  44.4 d  35.6 d  41.8 c  36.1 d
    Preventive -
    1 day
    ALC + 12 216.5 167.5 160.2 190.6 196.2 227.2 209.0 206.7
    Vinorelbine 44.3 41.6  60.6  70.2  49.5 b  72.6 d  56.9 c  59.8 d
    Therapeutic -
    9 days
    Student's “t” test vs Vinorelbine
    a = p ≦ 0.05,
    b = p ≦ 0.02,
    c = p ≦ 0.01
    d = p ≦ 0.001.
  • EXAMPLE 18 Protective Effect of Acetyl L-Carnitine on Peripheral Nerve from Vincristine-Induced Damage—Therapeutic and Preventive Treatment
  • The paw-withdrawal test (Randall-Sellitto) was used as in Example 16. Sprague Dawley male rats (Harlan) about 3 months of age (about 350 g) were used. Vincristine (0.150 mg/kg i.p.) in saline was administered three times a week until the end of the experiment.
  • The preventive treatment was conducted as follows: Vincristine 0.150 mg/kg i.p. three times a week+ALC 100 mg/Kg s.c., daily (6 days a week).
  • The therapeutic treatment was conducted as follows: Vincristine 0.150 mg/kg i.p.+ALC 100 mg/Kg s.c., daily (6 days a week) from 15th day of the Vincristine treatment.
  • The test was done twice a week.
  • The nociceptive threshold data (Randall-Sellitto) are given in the Table 21 and Table 22 here below.
  • TABLE 21
    Vincristine-induced neuropathy: nociceptive threshold (g) measured on
    the animals' hind paws (mean ± S.D.) - preventive treatment
    Days
    GROUP No 0 3 8 10 15 17 22
    Saline 30 212.7 220.0 202.0 221.3 213.3 213.3 222.0
    50.4  67.4 d  59.2 d  63.5 d  60.2 d  72.3 d  73.9 d
    Vincristine 30 222.0 137.3 114.7 117.3 126.7 94.7 133.0
    43.8  52.6  53.1  43.8  69.6  80.5  73.9
    ALC + Vincristine 30 210.7 206.7 209.3 213.3 213.3 202.7 215.0
    41.1  66.8 d  81.1 d  83.8 d  59.2 d  64.6 d  72.8 d
    Student's “t” test vs Vincristine
    d = p < 0.001.
  • TABLE 22
    Vincristine-induced neuropathy: nociceptive threshold (g) measured on
    the animals' hind paws (mean ± S.D.) - therapeutic treatment
    Days
    GROUP No 0 4 8 10 15 22 24 29 31 36 38
    Saline 10 197.2 233.5 201.5 209.4 224.8 231.0 223.5 215.9 222.2 250.4 248.6
    24.0  60.7 a 55.7  47.4 d  59.5 d  47.4 d  62.6 c  44.9 c  46.2 d  86.3 c  62.0 c
    Vincristine 10 199.4 173.6 145.6 131.4 127.4 120.6 133.0 128.0 129.2 137.8 154.2
    25.0  64.8 71.8  31.9  50.9  40.2  74.6  56.9  46.2  52.8  64.2
    ALC + 10 216.2 212.8 186.8 152.6 128.0 188.6 230.5 248.4 282.0 265.0 281.6
    Vincristine 38.9  62.9 87.3  59.5  39.8  72.4 b  68.9 c 100.2 c  76.8 d  71.5 d  97.1 c
    Student's “t” test vs Vincristine
    b = p ≦ 0.02,
    c = p ≦ 0.01
    d = p ≦ 0.001.
  • EXAMPLE 19 Study of the Influence of Acetyl L-Carnitine (ALC) Treatment on Bleomycin-Induced Pulmonary Toxicity
  • Hamsters weighing 120 g were treated with Bleomycin (1 unit) by the intratracheal route (IT) or with an equivalent volume of saline. In addition, the animals were pre-treated with ALC (200 mg/kg) or saline intraperitoneally just before the instillation of Bleomycin, followed by daily injections for 1 week. The animals were allowed 3 weeks to recover prior to taking the tissue samples. At the time tissue samples were taken (day 22), one lung of each animal was prepared for histological investigation and the other lung for quantitative determination of hydroxyproline.
  • The experimental groups were organized as follows:
  • 1. Pre-treatment with saline/saline IT
    2. Pre-treatment with saline/Bleomycin IT
    3. Pre-treatment with ALC/saline IT
    4. Pre-treatment with ALC/Bleomycin IT
  • Three experiments were carried out, in a total of 41 animals.
  • The lungs were fixed by insufflation at 20 cm of H2O ex vivo with 10% formaldehyde in PBS (pH 7), embedded in paraffin, sectioned and stained with haematoxylin/eosin. Sections with similar orientation from the portion of the pulmonary lobe—upper, middle and lower, respectively—were examined to verify the presence and degree of inflammatory infiltrate, oedema and interstitial and intra-alveolar fibrosis.
  • The lungs receiving saline or pre-treatment with ALC/saline IT presented a normal alveolar architecture. The animals treated with Bleomycin IT or with pre-treatment with saline alone presented dense fibrosis, alveolar atelectasis and oedema. In the animals pre-treated with ALC, there were a number of fibrotic areas, which, however, appeared thinner with less dense areas of collapse.
  • The hydroxyproline content (one of the main constituents of collagen) was measured using the known Woessner method. Lung samples were homogenised, hydrolysed with NaOH, and then left to oxidise before adding Erlich aldehyde reagent. Duplicate aliquots of each sample were assayed by spectrophotometry and compared with a standard calibration curve obtained with purified hydroxyproline. The hydroxyproline content (HYP) is expressed as mg/lung.
  • The untreated hamsters or those treated with ALC alone presented HYP levels compatible with normal accepted values. The animals treated with Bleomycin presented an increase in HYP consistent with the increased deposition of collagen during the fibrotic reaction. However, the animals treated with ALC showed a reduction in HYP as compared to those treated with Bleomycin, consistent with the improvement in the fibrotic response (Table 23).
  • TABLE 23
    Group HYP
    Saline/saline 0.741
    Saline/Bleomycin 1.831
    ALC/saline 0.801
    ALC/Bleomycin 1.380
  • ALC causes a reduction of the fibrotic response in animals treated with Bleomycin.
  • EXAMPLE 20 Increase in the Anticancer Activity of Taxol in the Presence of Propionyl L-Carnitine (PLC) In Vivo
  • Cell cultures and Tumour Inoculation Conditions
  • Cell cultures of L-MM3 murine breast cancer cells were used, cultured at 37° C. in plastic flasks in a humidified atmosphere with 5% CO2. The cells were grown in DMEM supplemented with 10% FCS and in the presence of 2 mM L-glutamine and 80 μg/ml of gentamicin. The subconfluent cells were collected during the exponential growth phase using trypsin-EDTA and re-suspended in DMEM. They were then injected subcutaneously in female Balb/c mice weighing 20 g at a density of 4×105.
  • Tumour Measurement Method
  • The tumour was measured with a calliper three times a week as soon as it became palpable. The tumour mass is calculated on the basis of the measurements of the two dimensions (length and width), expressed in mm, according to the following formula:
  • ( length × width 2 ) 2 = tumour volume ( mm 3 ) .
  • If we consider conventionally a tumour density equal to 1, the result is that the tumour volume is equal to (mm3=mg).
  • Taxol Preparation Method
  • Agent used: Taxol (paclitaxel INDENA). The agent is weighed, solubilised in the specific vehicle (12 mg/ml), and stored at +4° C., sheltered from the light. At the time of use, it is diluted 1:4 with saline solution in phosphate buffer (PBS, SIGMA) and injected.
  • Agent Vehicle: Cremophor EL (BASF).
  • Cremophor is diluted 1:1 with ethanol and stored sheltered from the light. On the day of treatment it is diluted 1:4 with PBS.
  • The animals were selected and treated as described in the previous examples.
  • Treatment Conditions
  • Schedule A). The mice were treated i.p. with 30 mg/kg of Taxol and s.c. with 100 mg/kg of PLC according to the following schedule.
  • Day Treatment
    A) 0 Inoculation of 400,000 cells/mouse
    12 Administration of 100 mg/kg s.c. of PLC
    13 PLC
    14 PLC
    15 PLC + Taxol (30 mg/kg)
    16 PLC
    17 PLC + Taxol
    18 PLC
    19 PLC + Taxol
    20 PLC
    21 PLC + Taxol
    22 PLC
    23 PLC
    24 PLC
    B) 0 Inoculation of 400,000 cells/mouse
    4 Administration of 100 mg/kg s.c. of PLC
    5 PLC
    6 PLC
    7 PLC
    8 PLC + Taxol 30 mg/kg i.p.
    9 PLC
    10 PLC + Taxol
    11 PLC
    12 PLC + Taxol
    13 PLC
    14 PLC + Taxol
    15 PLC
    59 PLC
  • In both treatment schedules, the control, Taxol and PLC groups were inoculated with the same number of cells.
  • In addition, the Taxol treatment was given according to the same procedures and at the same times both in the group treated with Taxol alone and in the one treated with Taxol and PLC.
  • Treatment with PLC, whether alone or in combination with Taxol, in treatment schedule A) starts on day 12/after inoculation of the tumour) and ends on day 24; in treatment schedule B), the treatment starts on day 5 after inoculation of the tumour and ends at the end of the experiment, i.e. on day 59.
  • Results
  • Experiment A)
    Animals with tumours/total number of animals
    Day Control Taxol Taxol + PLC PLC
    19  8/13 6/13 4/12  4/12
    22 10/13 6/13 6/12  6/12
    25 11/13 7/13 6/12  9/12
    28 12/13 9/13 6/12 11/12
    36 13/13 11/13  7/12 12/12
    46 13/13 11/13  8/12 12/12
    Tumour size
    Day Control SE Taxol + PLC Taxol + PLC PLC
     0 0 0 0 0
    22 2.30 ± 0.23 0.8 ± 0.4 0.4 ± 0.3  0.6 ± 0.3
    25   3 ± 0.6 0.96 ± 0.4  0.5 ± 0.32  1.2 ± 0.38
    28 3.9 ± 0.6 1.4 ± 0.4 0.8 ± 0.52   3 ± 0.4
    36 9.5 ± 0.6 5.5 ± 0.5 3.4 ± 1.25 9.5 ± 0.8
    46 14.3 ± 0.86  11 ± 1.5 7.6 ± 2.15  15 ± 1.1
  • On applying the non-parametric Mann-Whitney test for unpaired data, significant differences were found at all observation times in the Taxol+PLC group versus the control group, with p<0.003, and only at the last observation time (day 46) did the significance level drop to p<0.034. It should be noted that the values for the Taxol group on day 46 were not significantly different from the control group values.
  • Experiment B)
    Day Control Taxol Taxol + PLC PLC
    Animals with tumours/total animals
    31 4/11 2/10 1/10 3/10
    45 8/11 5/10 4/10 8/10
    59 10/11  6/10 6/10 8/10
    Tumour size
    0 0 0  0 0
    26 0.4 ± 0.2 0.1 ± 0.6 000 0.4 ± 0.3
    31 0.6 ± 0.3 0.2 ± 0.2 000 0.5 ± 0.3
    37 1.9 ± 0.8 0.45 ± 0.2  0.050 ± 0.050 1.3 ± 0.6
    41 3.1 ± 1.3 1.750 ± 1.1   0.1 ± 0.060 2.2 ± 1.0
    45 3.59 ± 1.3  2.250 ± 1.13  0.4 ± 0.2 3.9 ± 1.2
    53 7.2 ± 2   5.000 ± 2.2    2 ± 0.8 5.6 ± 1.4
    59 9.6 ± 1.9 5.6 ± 2.2 3.950 ± 1.5    8 ± 2.2
  • The Wilcoxon statistical test was applied in this experiment, which revealed that only the control group was significantly different from the Taxol+PLC group, with p<0.05.
  • EXAMPLE 21
  • Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection. Cells were cultured in RPMI (Sigma Chemicals, Aldrich) supplemented with 5% heat-inactivated horse serum (Gibco, Milan, Italy), 5% heat-inactivated foetal calf serum and 1% PNS antibiotic mixture (Gibco, Milan, Italy) in a humidified incubator (Heraeus, Angelantoni, Italy) at 37° C. with 5% CO2 atmosphere. PC12 cells were seeded in 6 wells plates (containing a polysilylated cover slide) at the density of 150.000 cells/well. After 6 hrs cells were treated with NGF 100 ng/ml for 72 h to induce neuronal differentiation and neurite outgrowth. At the end of the period of incubation the medium was replaced and the differentiated cells were treated for 24 h with 100 ng/ml NGF+/−Taxol (1 μg/ml) or cisplatin (10 μg/ml)+/−ALC (1 mM). PC12 cells were then fixed using the methanol-acetone method: cells grown on the cover slides were washed twice with PBS 1×, then treated with chilled methanol (−20° C.) and incubated at +4° C. for 2 min. Then, methanol was flushed out and chilled acetone (−20° C.) was put on to the cells. After 5 min, acetone was washed out and cells were treated with 2 ml of 1% Triton X-100, 0.5% acetic acid in PBS 1× for 10 min at room temperature. Cells were washed twice with PBS 1× and incubated for 5 h with specific anti-acetylated α-tubulin antibody (Sigma Immunochemicals, Milan, Italy) diluted 1:10 in PBS at room temperature in humid chamber. Following two rinses in PBS, cells were incubated overnight with FITC-labelled goat anti-mouse IgG antibody (Vector, Burlingame, Calif.) diluted 1:10 in PBS at room temperature in order to localize the neurites of differentiated cells and with Hoechst 1× for 10 min at room temperature to stain nuclei. The number of neurites of differentiated cells, i.e. those longer than 0.35 μm, was determined. Slides were analyzed with a Zeiss III photomicroscope with epifluorescence equipment using the 09 combination of filters (BP 450-490, FT 510, LP420 or BP 546). Analysis was performed on 25 fields/treatment.
  • The percentage of neurites of differentiated cells (i.e. neurites longer than 0.35 μm) was drastically decreased in Taxol-treated cells with respect to the controls (7% vs. 39%, respectively p<0.001). The reduction in the percentage of differentiated cells was evident also in the cisplatin-treated cells vs. controls, although to a lesser extent (19% vs. 39% respectively; p<0.01). Conversely, the ALC co-treatment with Taxol or cisplatin reduced the degeneration of the elongated neurites (differences were not significant respect to the control group). No differences between the controls and ALC treated cells were observed.
  • EXAMPLE 22 Protective Effect of Acetyl L-Carnitine on an Experimental Model of Cisplatin-Induced Peripheral Neuropathy—NGF Involvement
  • The purpose of this study is to evaluate NGF circulating levels in relation to Cisplatin induced peripheral neuropathy, and to assess the effect of acetyl L-carnitine administered in the co-ordinated use with Cisplatin.
  • Peripheral neuropathy was induced by intraperitoneally administration of Cisplatin (2 mg/kg ml) dissolved in distilled sterilized water, once a week for 8 weeks (for a total of 8 administrations).
  • Acetyl L-carnitine was administered orally at the dose of 100 mg/Kg/day. ALC treatment occurred daily, starting 5 days before Cisplatin.
  • The nerve conduction velocity on the caudal nerve was determined in baseline condition and at the end of the treatment period as in Example 2; data measured are given in the Table 24 here below.
  • TABLE 24
    Cisplatin-induced neuropathy: sensory conduction velocity
    in m/s measured on the caudal nerve in basal conditions
    and after treatment with acetyl L-carnitine.
    TREATMENT
    GROUP BASAL Cisplatin 8th treatment
    Vehicle 31.1 ± 1.8 41.1 ± 3.0
    Cisplatin 32.9 ± 1.4 27.8 ± 1.9§§
    ALC + Cisplatin 32.8 ± 1.6 33.4 ± 2.8§*
    Values are means ± standard deviation.
    1-way ANOVA with Neuman-Keuls' post hoc test
    §= P < 0.05 vs Vehicle
    §§= P < 0.01 vs Vehicle
    *= p < 0.05 vs Cisplatin
  • After the last determination of nerve conduction velocity, the NGF circulating levels were determined: 3 ml of whole blood were obtained from each animal through abdominal aorta puncture and collected into a heparinated tube. Plasma was obtained and immediately stored at −80° until used for NGF level determination. The circulating levels of NGF were measured by ELISA using commercially available kits (Emax™ ImmunoAssay System, Promega, USA; plasma working dilution for NGF 1:80) following the manufacturer protocols. NGF circulating levels are given in the Table 25 here below.
  • TABLE 25
    NGF plasma concentrations (ng/ml)
    GROUP NGF
    Vehicle 69.4 ± 12.7
    Cisplatin 42.7 ± 7.1§§
    ALC + Cisplatin 58.1 ± 15.3*
    Values are means ± standard deviation.
    1-way ANOVA with Neuman-Keuls' post hoc test
    §§= P < 0.01 vs Vehicle
    *= p < 0.05 vs Cisplatin

Claims (19)

1.-27. (canceled)
28. A method of preventing and/or treating peripheral neuropathies induced by the administration of oxaliplatin comprising administering to a subject acetyl L-carnitine or a pharmaceutically acceptable salt thereof and said oxaliplatin.
29. The method according to claim 28, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a co-ordinated manner.
30. The method according to claim 29, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a sequential manner.
31. The method according to claim 29, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a substantially simultaneous manner.
32. The method according to claim 28, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in the form of a composition comprising said acetyl L-carnitine or said pharmaceutically acceptable salt and said oxaliplatin and optionally pharmaceutically acceptable excipients and/or vehicles.
33. The method according to claim 28, wherein said pharmacologically acceptable salt of acetyl L-carnitine is selected from the group consisting of chloride, bromide, orotate, acid aspartate, acid citrate, acid phosphate, fumarate and acid fumarate, maleate and acid maleate, acid oxalate, acid sulphate, glucose phosphate, tartrate and acid tartrate.
34. The method according to claim 28, wherein said acetyl L-carnitine is administered to a subject in need of a treatment with oxaliplatin.
35. The method according to claim 34, wherein said oxaliplatin and said acetyl L-carnitine are administered immediately before or immediately after surgical removal of a tumor.
36. The method according to claim 34, wherein said oxaliplatin is alternative to surgical removal of the tumor.
37. A method of preventing and/or treating peripheral neuropathies induced by the administration of oxaliplatin comprising administering to a subject expected to suffer or suffering from said peripheral neuropathies an effective amount of acetyl L-carnitine or a pharmaceutically acceptable salt thereof and said oxaliplatin.
38. The method according to claim 37, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a co-ordinated manner.
39. The method according to claim 37, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a sequential manner.
40. The method according to claim 37, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a substantially simultaneous manner.
41. A method of preventing peripheral neuropathies induced by the administration of oxaliplatin comprising administering to a subject in view of receiving a treatment with oxaliplatin an effective amount of acetyl L-carnitine or a pharmaceutically acceptable salt thereof before receiving said oxaliplatin.
42. A method of preventing peripheral neuropathies induced by the administration of oxaliplatin comprising administering to a subject in view of receiving a treatment with oxaliplatin an effective amount of acetyl L-carnitine or a pharmaceutically acceptable salt thereof immediately before or immediately after surgical removal of the tumor and before receiving said oxaliplatin.
43. A method of treating peripheral neuropathies induced by the administration of oxaliplatin comprising administering to a subject receiving a treatment with oxaliplatin an effective amount of acetyl L-carnitine or a pharmaceutically acceptable salt thereof.
44. The method according to claim 43, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a sequential manner.
45. The method according to claim 43, wherein said oxaliplatin and said acetyl L-carnitine or said pharmaceutically acceptable salt are administered in a substantially simultaneous manner.
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WO2018008973A1 (en) * 2016-07-06 2018-01-11 한국 한의학 연구원 Composition comprising forsythiae fructus extract as effective ingredient for preventing, ameliorating or treating peripheral neuropathy

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US20080194683A1 (en) 2008-08-14
EP1100589A2 (en) 2001-05-23
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AU764807B2 (en) 2003-08-28
KR100645980B1 (en) 2006-11-14
HK1035677A1 (en) 2001-12-07
ATE287278T1 (en) 2005-02-15
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JP4703851B2 (en) 2011-06-15
CA2338638A1 (en) 2000-02-10
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US20060258744A1 (en) 2006-11-16
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EP1100589B1 (en) 2005-01-19
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