US20110126913A1 - Device Including A Dissolvable Structure For Flow Control - Google Patents
Device Including A Dissolvable Structure For Flow Control Download PDFInfo
- Publication number
- US20110126913A1 US20110126913A1 US12/954,519 US95451910A US2011126913A1 US 20110126913 A1 US20110126913 A1 US 20110126913A1 US 95451910 A US95451910 A US 95451910A US 2011126913 A1 US2011126913 A1 US 2011126913A1
- Authority
- US
- United States
- Prior art keywords
- fluid
- retainment
- integer
- independently
- fluid processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012530 fluid Substances 0.000 claims abstract description 297
- 238000012545 processing Methods 0.000 claims abstract description 137
- 239000000463 material Substances 0.000 claims abstract description 67
- 230000000717 retained effect Effects 0.000 claims abstract description 12
- 229920001223 polyethylene glycol Polymers 0.000 claims description 59
- 238000004891 communication Methods 0.000 claims description 51
- 239000002202 Polyethylene glycol Substances 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 37
- 238000003556 assay Methods 0.000 claims description 13
- CBOIHMRHGLHBPB-UHFFFAOYSA-N hydroxymethyl Chemical compound O[CH2] CBOIHMRHGLHBPB-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000003825 pressing Methods 0.000 claims description 8
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 230000005012 migration Effects 0.000 claims description 4
- 238000013508 migration Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 83
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 67
- 230000004888 barrier function Effects 0.000 abstract description 24
- 239000000523 sample Substances 0.000 description 47
- 239000000243 solution Substances 0.000 description 33
- -1 Poly(ethylene glycol) Polymers 0.000 description 32
- 239000000758 substrate Substances 0.000 description 26
- 239000002699 waste material Substances 0.000 description 22
- 230000008569 process Effects 0.000 description 15
- 239000000126 substance Substances 0.000 description 12
- 230000008859 change Effects 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000003993 interaction Effects 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000011534 wash buffer Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000009172 bursting Effects 0.000 description 4
- 239000010410 layer Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- HBXWUCXDUUJDRB-UHFFFAOYSA-N 1-octadecoxyoctadecane Chemical compound CCCCCCCCCCCCCCCCCCOCCCCCCCCCCCCCCCCCC HBXWUCXDUUJDRB-UHFFFAOYSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- FDCJDKXCCYFOCV-UHFFFAOYSA-N 1-hexadecoxyhexadecane Chemical compound CCCCCCCCCCCCCCCCOCCCCCCCCCCCCCCCC FDCJDKXCCYFOCV-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 239000012790 adhesive layer Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 150000002334 glycols Chemical class 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 150000007523 nucleic acids Chemical group 0.000 description 2
- 229920001983 poloxamer Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 229920000428 triblock copolymer Polymers 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 229920002012 Pluronic® F 38 Polymers 0.000 description 1
- 229920002021 Pluronic® F 77 Polymers 0.000 description 1
- 229920002023 Pluronic® F 87 Polymers 0.000 description 1
- 229920002025 Pluronic® F 88 Polymers 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229920001427 mPEG Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 229920001992 poloxamer 407 Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
- F16K99/003—Valves for single use only
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0034—Operating means specially adapted for microvalves
- F16K99/0036—Operating means specially adapted for microvalves operated by temperature variations
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0034—Operating means specially adapted for microvalves
- F16K99/0055—Operating means specially adapted for microvalves actuated by fluids
- F16K99/0057—Operating means specially adapted for microvalves actuated by fluids the fluid being the circulating fluid itself, e.g. check valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0621—Control of the sequence of chambers filled or emptied
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0661—Valves, specific forms thereof with moving parts shape memory polymer valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
- B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/082—Active control of flow resistance, e.g. flow controllers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0082—Microvalves adapted for a particular use
- F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S422/00—Chemical apparatus and process disinfecting, deodorizing, preserving, or sterilizing
- Y10S422/901—Polymer dissolver
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0324—With control of flow by a condition or characteristic of a fluid
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/1624—Destructible or deformable element controlled
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/1624—Destructible or deformable element controlled
- Y10T137/1797—Heat destructible or fusible
- Y10T137/1812—In fluid flow path
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/206—Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system]
- Y10T137/218—Means to regulate or vary operation of device
- Y10T137/2191—By non-fluid energy field affecting input [e.g., transducer]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8376—Combined
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/8593—Systems
- Y10T137/85978—With pump
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Definitions
- FIG. 8 illustrates the effect of misalignment of the pieces shown in FIG. 6 for an embodiment of the device as shown in FIG. 2 .
- a fluid processing device can comprise one or more fluid processing passageways that can comprise one or more elements, for example, one or more of a channel, a branch channel, a valve, a flow splitter, a vent, a port, an access area, a via, a bead, a reagent containing bead, a cover layer, a reaction component, any combination thereof, and the like. Any element can be in fluid communication with another element.
- the fluid flow modulator can be in the form of a valve that only partially blocks fluid flow through the fluid processing passageway.
- Exemplary derivatives of PEG can include those shown in the Table 2 below:
- the material that makes up the solute bridge valve 22 is a material that dissolves into the fluids 30 , 32 .
- the material of the solute bridge valve also is desirably compatible with the assay to be conducted, and would not adversely affect the assay condition.
- the solute bridge valve material could also be an active ingredient that might catalyze or react with constituents of the assay.
- Examples of material that can be used to make up the solute bridge valve 22 include polyethyleneglycol (PEG) and derivatives of polyethyleneglycol, together referred to herein as PEG.
- PEG has desirable properties and some PEG materials can dissolve in aqueous liquids, such as those typically used in many biological assays. PEG is generally inert and generally does not affect biological assays.
- Reagent retainment regions 40 , 42 can be selectively separated from the intermediate retainment regions 44 , 46 by the pressure actuated valves 60 , 62 placed within the fluid flow passages 160 , 162 .
- the pressure actuated valves 60 , 62 within fluid processing passageways 160 , 162 can be diaphragms that are burstable upon pressure being applied to the reagent retainment regions 40 , 42 .
Abstract
A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.
Description
- This application claims benefit under 35 U.S.C. Section 119(e) from earlier U.S. Provisional Patent Applications Nos. 60/619,731, 60/619,677, and 60/619,623, all of which were filed Oct. 18, 2004, and all of which are incorporated herein in their entireties by reference.
- The present teachings relate to a diagnostic device and method providing automatically controlled interconnection between a plurality of retainment regions.
- A portable or nonportable diagnostic device or method can perform a set of predetermined assays by providing for controlled interaction between various fluids initially present in separate retainment regions. A device and method compatible with nucleic acid sequence reactions, and detecting such reactions, is desirable.
- According to various embodiments, a diagnostic device is provided that includes a plurality of retainment regions, as exemplified below, with the retainment regions being interconnected through a plurality of fluid communications, fluid processing passageways, and/or channels. Herein the phrase “retainment region” means a retainment or containment feature such as a well, a fluid retainment region, a reservoir, a channel, a vial, a compartment, another receptacle, a surface on which a material is retained, or the like. The following discussion with regard to retainment regions would be equally applicable to any of the above-mentioned features or their equivalents.
- The retainment regions can include a reaction retainment region, one or more reagent retainment regions each containing reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each of the fluid processing passageways interconnecting the one or more reagent retainment regions with respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. A barrier or fluid flow modulator, as exemplified below with reference to a valve, can be provided in one or more of the fluid processing passageways interconnecting the reagent retainment regions with the reaction retainment region or intermediate retainment regions, or interconnecting the intermediate retainment regions and the reaction retainment region.
- According to various embodiments, a method of performing a set of predetermined assays is provided. The method can include providing a plurality of retainment regions in a closed, and if desired, disposable cuvette, with the retainment regions being interconnected by fluid processing passageways but closed to fluid flow to or from locations outside of the cuvette. First retainment regions can be selectively closed off from fluid communication with second retainment regions with which they are interconnected by first channels including pressure-actuated valves positioned therein. The pressure-actuated valves can comprise a burstable or tearable diaphragm, or other frangible seal that can rupture, tear, break, or the like, when exposed to a change in pressure, for example, an increase or decrease in pressure. One or more third retainment regions can be selectively closed off from fluid communication with at least the second retainment regions with which they are interconnected by second channels by valves positioned in the second channels. Pressure can be applied to the pressure actuated valves in the first channel sufficient to provide fluid communication between the first and second retainment regions. A sample to be tested or otherwise processed can be introduced into the one or more third retainment regions, and fluid communication can be established between the second retainment regions and the one or more third retainment regions at a controlled rate that can be a function of any one of a number of stimuli and/or characteristics of at least one of the sample in the one or more third retainment regions and a fluid within the second retainment regions. The characteristics of at least one of the sample in the one or more third retainment regions and a fluid within the second retainment region can include, but are not limited to water content, pH, chemical composition, temperature, electrical charge, magnetic properties, or the like.
- The closed, and if desired, disposable cuvette, can be provided as a substrate that is fabricated from a single piece or more than one piece. The retainment regions, interconnecting fluid processing passageways, and/or valves can be fabricated all in the single piece substrate, or if desired, can be fabricated in one or more different pieces, which can then be combined to form the cuvette.
- According to some embodiments, the device can comprise no vent, at least one vent, or a plurality of vents, to relieve pressure resulting from a flow of a fluid and its communication. A vent can comprise a vent channel configured to relieve such pressure. A vent can be provided in communication with a retainment region, such that upon fluid flow resultant pressure is released. A vent channel can comprise a hydrophobic vent channel that allows air to travel through the channel but does not allow the flow of an aqueous fluid.
- According to some embodiments, a device is provided that can comprise no vent and can be manufactured and sealed under vacuum whereby the device can comprise a low internal gas pressure relative to the external ambient pressure.
- Additional features and advantages of various embodiments will be set forth in part in the description that follows, and in part will be apparent from the description, or can be learned by practice of various embodiments. Other advantages of the various embodiments will be realized and attained by means of the elements and combinations exemplified in the application.
- Various embodiments of the present teachings are exemplified in the accompanying drawings. The teachings are not limited to the embodiments depicted in the drawings, and include equivalent structures and methods, as set forth in the following description and as would be known to those of ordinary skill in the art in view of the present teachings.
-
FIGS. 1( a), 1(b), and 1(c) schematically illustrate various stages in the operation of a valve according to various embodiments. -
FIG. 2 is a schematic illustration of a diagnostic device according to various embodiments. -
FIG. 3 is a schematic illustration of a diagnostic device according to various embodiments. -
FIGS. 4A-4J schematically illustrate various stages in the operation of a diagnostic device according to an embodiment. -
FIGS. 5A-5J schematically illustrate various stages in the operation of a diagnostic device according to an embodiment. -
FIG. 6 illustrates the assembly of a diagnostic device from two separate pieces. -
FIG. 7 illustrates the effect of misalignment of the pieces shown inFIG. 6 for an embodiment of the device as shown inFIG. 3 . -
FIG. 8 illustrates the effect of misalignment of the pieces shown inFIG. 6 for an embodiment of the device as shown inFIG. 2 . -
FIG. 9 illustrates an arrangement of retainment regions and a valve according to various embodiments. -
FIG. 10 illustrates an arrangement of retainment regions and a valve according to various embodiments. -
FIG. 11 illustrates an arrangement of retainment regions and a valve according to various embodiments. - It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are intended to provide a further explanation of the various embodiments of the present teachings.
- According to various embodiments, a diagnostic device, that can be either portable or nonportable, is provided to perform one or more predetermined assays as desired, for example, in nucleic acid sequence detection technology. For a given assay, the assay protocol can involve a set of fluid handling steps such as mixing, incubation, washing, and the like, which are desirably performed in a given sequence of steps and for specified time periods for samples and reagents in specified volumes or proportions. The device can be miniaturized to the point that it can be used as a handheld portable diagnostic device. As shown in the exemplary embodiments illustrated in
FIG. 2 andFIG. 3 , the diagnostic device can include a plurality of retainment regions, as exemplified below, interconnected by fluid processing passageways. The plurality of retainment regions can be included within a closed, disposable cuvette such that all of the retainment regions are closed to fluid flow to or from locations outside of the cuvette. The plurality of retainment regions in the cuvette can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region that can contain or receive a sample to be reacted with the unreacted reagents. The unreacted reagents, which can include enzymes, buffers, catalysts, or other reaction components, can be contained in a first set of retainment regions that are interconnected with an intermediate set of retainment regions by fluid processing passageways containing pressure-actuated valves. The intermediate retainment regions can also be connected through fluid processing passageways to a reaction retainment region, with the fluid processing passageways that connect the intermediate retainment regions to the reaction retainment region comprising a material that is adapted to reduce in volume within the fluid processing passageway when brought into contact with fluids from the intermediate retainment regions, or when brought into contact with a sample after the sample has been added to the sample retainment region. - The term “fluid processing passageway” means any area, a structure, or communication, that allows for fluid communication between at least two retainment regions, for example, a channel connecting two regions. One or more fluid processing passageways according to the present teachings can be configured or adapted to provide capillary driven flow. One or more fluid processing passageways according to the present teachings can be configured or adapted to provide electrokinetic driven flow. One or more of the fluid processing passageways according to the present teachings can be configured or adapted to control the rate and timing of fluid flow by varying the dimensions of the fluid processing passageway.
- The terms “fluid processing passageway,” “a fluid communication,” “fluid flow channel,” “fluid processing passageway,” “flow channel,” “flow control channel,” and “flow control passageway,” are each used synonymous with the term “fluid passageway,” as herein defined.
- According to various embodiments, the term “fluid” means a gas, an aqueous fluid, a non-aqueous fluid, a vacuum, or a partial vacuum. A gas can comprise, for example, air. Where two retainment regions are separated by a fluid flow modulator, one retainment region can comprise, for example, an aqueous or non-aqueous fluid retained therein, while the other retainment region can comprise a gas or a vacuum or partial vacuum, contained therein. In various embodiments, the device can be manufactured to provide a vacuum on one or more sides of a dissolvable valve, for example, to achieve a pressure of from about 0.01 to about 0.99 atm, or from about 0.1 to about 0.5 atm.
- The term “retainment region” means any area that can comprise a reagent or other reaction component for a reaction where the retainment region is in fluid communication with, fully separate from, or partially separate from, another retainment region that can comprise another reagent or reaction component for the reaction that is the same as or different from the first reagent. A first retainment region can be separate from a second retainment region, or a first retainment region can be surrounded by a second retainment region, where the first and second retainment regions are separated by a barrier comprising a shaped-wall.
- A retainment region can comprise any area, structure, or form, capable of retaining a volume of fluid. A retainment region can be used, for example, to retain, process, react, store, incubate, transfer, purify, or the like, a fluid sample. A retainment region can comprise a surface area, an area, a recess, a reservoir, a chamber, a depression, a well, a space, or the like. According to some embodiments, a retainment region can comprise, for example, a flat surfaces with hydrophobic regions surrounding hydrophilic loci for receiving, containing, retaining, or binding a sample. A retainment region can comprise any shape, for example, round, teardrop, square, polygon, star, irregular, ovoid, rectangular, or the like. A retainment region or fluid processing passageway can comprise any cross-section configuration, for example, square, round, ovoid, irregular, trapezoid, or the like.
- The terms “reservoir,” “retainment region,” and “region,” are used synonymously herein.
- The term “reagent for reaction,” means one or more reagents or components necessary or desirable for use in one or more reactions or processes, for example, one or more components that in any way affect how a desired reaction can proceed. The reagent for reaction can comprise a reactive component. However, it is not necessary that the reagent participate in the reaction. The reagent for reaction can comprise a non-reactive component. The reagent for reaction can comprise a recoverable component comprising for example, a solvent and/or a catalyst. The reagent for reaction can comprise a promoter, accelerant, or retardant that is not necessary for a reaction but affects the reaction, for example, affects the rate of the reaction. The reagent for reaction can comprise one or more of a solid reagent for reaction and a fluid reagent for reaction. The term “reaction component” is used synonymous with the term “reagent for reaction,” as herein defined. The reagent for reaction can comprise one or more of a fluid and a solid. A retainment region can be pre-loaded with one or more reagents for reaction.
- The term “vent” means any configuration or structure that relieves vacuum and/or back pressure, or equalizes pressure in a fluid processing device. A vent can comprise a channel or a microchannel. A vent can comprise a non-flow through vent in which gas that is displaced by a fluid can collect. A non-flow through vent can comprise, for example, a hydrophobic vent.
- According to various embodiments, suitable reactions or processes can comprise one or more of a sample preparation process, a washing process, a sample purification process, a pre-amplification process, a pre-amplified product purification process, an amplification process, an amplified product purification process, a separation process, a sequencing process, a sequencing product purification process, a labeling process, a detecting process, or the like. Processing components can comprise sample preparation components, purification components, pre-amplification reaction components, amplification reaction components, sequencing reaction components, or the like. The skilled artisan can readily select and employ suitable components for a desired reaction or process, without undue experimentation.
- According to some embodiments, processing or reaction components can be disposed in one or more retainment regions, channels, or fluid processing passageways, using any methods known in the art. For example, components can be sprayed and dried, delivered using a diluent, injected using a capillary, a pipette, and/or a robotic pipette, or otherwise disposed in the regions or fluid processing passageways.
- According to various embodiments, a fluid processing device is provided that can comprise one or more fluid processing passageways that can comprise one or more elements, for example, one or more of a channel, a branch channel, a valve, a flow splitter, a vent, a port, an access area, a via, a bead, a reagent containing bead, a cover layer, a reaction component, any combination thereof, and the like. Any element can be in fluid communication with another element.
- The term “fluid communication” means either direct fluid communication, for example, two regions can be in fluid communication with each other via an unobstructed fluid processing passageway connecting the two regions or can be capable of being in fluid communication, for example, two regions can be capable of fluid communication with each other when they are connected via a fluid processing passageway that can comprise a valve disposed therein, wherein fluid communication can be established between the two regions upon actuating the valve, for example, by dissolving a dissolvable valve disposed in the fluid processing passageway.
- The term “in fluid communication” refers to in direct fluid communication and/or capable of direct fluid communication, unless otherwise expressly stated. The term “in valved fluid communication” refers to elements wherein a valve is disposed between the elements, such that upon opening or actuating the valve, fluid communication between the elements is established.
- According to some embodiments, the term “capillary flow” means passive flow resulting from a capillary potential gradient or a surface potential gradient, created during device fabrication that can direct the flow of liquid via capillary effect (surface tension).
- According to some embodiments a fluid processing device is provided. The device can comprise a substrate that can comprise, for example, a top or a first surface, and one or more fluid processing passageways that can be provided in communication with and/or can be defined by, for example, at least a portion of the top or first surface of the substrate. The one or more fluid processing passageways can be provided, for example, in a top or first surface of a substrate, on a top or first surface of a substrate, in a substrate, in a bottom or second surface of a substrate, on a bottom or second surface of a substrate, in an edge of a substrate, on an edge of a substrate, or any combination thereof. A fluid processing device can comprise different levels and layers of fluid processing passageways that can comprise, for example, different levels and layers of fluid processing passageways and regions. For example, a tiered, multi-channel device can comprise one or more fluid processing passageways that traverse different heights or levels in the substrate.
- Throughout the application, descriptions of various embodiments use “comprising” language; however, it will be understood by one of skill in the art, that in some specific instances, an embodiment can alternatively be described using the language “consisting essentially of or “consisting of.”
- For purposes of better understanding the present teachings and in no way limiting the scope of the teachings, it will be clear to one of skill in the art that the use of the singular includes the plural unless specifically stated otherwise. Therefore, the terms “a,” “an” and “at least one” are used interchangeably in this application.
- Unless otherwise indicated, all numbers expressing quantities, percentages or proportions, and other numerical values used in the specification and claims, are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained. In some instances, “about” can be understood to mean a given value ±5%. Therefore, for example, about 100 nl, could mean 95-105 nl. At the very least, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.
- As used herein, the term “plurality” means “two or more.” Herein, the term “two or more” is used synonymously with the term “plurality.”
- A user can operate the diagnostic device by injecting a sample into the sample retainment region, prior to, at the same time as, or subsequent to pushing a button or otherwise applying pressure to the retainment regions that contain unreacted reagents. For example, a user can inject a sample and then push a button or other feature or area of the device. As an example of an assay performed with a device according to various embodiments, a typical ligation assay for detection of oligo-nucleotides can include constituents comprising the sample, a ligation oligomer, ligation reagent which can be a mixture of enzyme and buffer, a wash buffer, and extension and detection reagents. The sample, the ligation oligomer, and the ligation reagent can be allowed to mix and react along with wash buffers and the extension and detection reagents in an automatically controlled process. The process can occur after a user has injected the sample into the sample retainment region and has released the reagents from the unreacted reagent retainment regions by applying pressure to those retainment regions.
- According to various embodiments, a diagnostic device can be provided that uses capillary driven flow for fluid actuation. The flow cross-section of the fluid processing passageways interconnecting the various retainment regions can contribute to the rate at which the reagents and sample are mixed in the reaction retainment region. Valves placed within the flow control fluid processing passageways interconnecting the retainment regions can provide automatic flow control and timing of the fluid actuation.
- According to various embodiments, a fluid flow modulator, as exemplified below with reference to a valve in a flow control passageway interconnecting retainment regions, can comprise a material that dissolves when brought into contact with a fluid having desired characteristics. Herein, the phrase “dissolvable valve” will be used interchangeably with the phrase “solute bridge valve.” The solute bridge valve can automatically control flow through the fluid processing passageway interconnecting the retainment regions and control the timing of fluid actuation by exploiting the time it takes to dissolve, melt, or otherwise wash-away or reduce the volume of the material making up the solute bridge valve.
- According to various embodiments, the fluid processing device can comprise a fluid processing passageway, a plurality of retainment regions with at least two of the retainment regions each being in fluid communication with the fluid processing passageway, and a fluid flow modulator arranged in the fluid processing passageway and adapted to open and form, or to increase in size, a fluid communication between the at least two retainment regions. The fluid flow modulator can comprise at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, or a combination thereof. The fluid flow modulator can comprise a material that is adapted to dissolve when contacted with water at room temperature. At least one of the plurality of retainment regions can comprise an aqueous fluid retained therein.
- According to various embodiments, the fluid processing device comprises a fluid flow modulator in the form of a valve. The valve can block fluid flow through a fluid processing passageway.
- The fluid flow modulator can be in the form of a valve that only partially blocks fluid flow through the fluid processing passageway.
- According to various embodiments, the fluid flow modulator can comprise at least one of a polyethylene glycol material and a derivative of a polyethylene glycol material, having a molecular weight of from about 500 Daltons to about 5,000,000 Daltons. The fluid flow modulator can comprise at least one of a polyethylene glycol material and a derivative of a polyethylene glycol material, having a melting point of from about 35° C. and about 65° C.
- According to various embodiments, the fluid processing device comprises a fluid processing passageway dimensioned so that a flow of fluid from at least one of two or more retainment regions and through the fluid processing passageway, can occur by capillary action. One or more maximum dimensions of about 5 millimeter or less, for example, about 2 millimeters or less, or about 1 millimeter or less.
- According to various embodiments, the fluid processing device comprises a fluid processing passageway dimensioned so that a migration of charged components in a fluid, from at least one of the retainment regions through the fluid processing passageway, is capable of migration by electrokinetic action. One or more maximum dimensions of about 5 millimeter or less, for example, about 2 millimeters or less, or about 1 millimeter or less.
- According to various embodiments, the fluid processing device can comprise at least two electrodes disposed in the device with a fluid processing passageway therebetween. A system can be provided that includes electrical leads that can be electrically connected to the electrodes.
- According to various embodiments, the fluid processing device can further comprise at least one additional retainment region, at least one additional fluid processing passageway, and at least one pressure-actuatable valve arranged in the at least one additional fluid processing passageway. The additional fluid processing passageway can be in fluid communication with the additional retainment region and one or more other retainment regions. The pressure-actuatable valve can comprise a frangible diaphragm. The frangible diaphragm can comprise a material that is insoluble in water at room temperature. The pressure-actuatable valve can comprise a burstable valve that is adapted to open and establish fluid communication only upon receiving pressure of at least about 0.1 psig, for example, at least about 0.5 psig, at least about 1 psig, or at least about 3 psig from a fluid in at least one additional retainment region. The device cam comprise a liquid retained in at least one additional retainment region.
- According to various embodiments, the fluid processing device can comprise at least one heat-actuatable valve arranged in at least one additional fluid processing passageway. The at least one additional fluid processing passageway can be in fluid communication with at least one additional retainment region and at least one of the plurality of retainment regions. The heat-actuatable valve can comprise at least one material selected from a rubber, a plastic, a wax, a paraffin, a polyethylene glycol material, a derivative of a polyethylene glycol material, a polysaccharide, a derivative of polysaccharide, and combinations thereof. The heat-actuatable valve can comprise a material that is insoluble in water at room temperature. The heat-actuatable valve can comprise a material that has a melting point of from about 35° C. to about 95° C., for example, froth about 35° C. to about 70° C., from about 35° C. to about 65° C., or from about 35° C. to about 50° C.
- According to various embodiments, the fluid processing device can comprise a liquid retained in at least one retainment region. The fluid processing device can comprise a first reagent for a reaction, retained in at least a first one of the plurality of retainment regions. The fluid processing device can comprise a second reagent for the reaction retained in at least a second one of the plurality of retainment regions. The second reagent can be the same as, or can differ from, the first reagent.
- According to various embodiments, the fluid processing device comprises a fluid flow modulator that comprises a substituted polyethylene glycol material. An exemplary substituted polyethylene glycol comprises poly (ethylene glycol) methyl ether. The fluid flow modulator comprises a polyethylene glycol derivative. An exemplary polyethylene glycol derivative can comprise a triblock copolymer of polyethylene oxide and polypropylene oxide. The fluid flow modulator can comprise a branched polyethylene glycol or derivative thereof. Exemplary substituted polyethylene glycol materials are shown in Table 1 below:
-
TABLE 1 Examples for Substituted Poly(ethylene glycol)s mp ca. # Trade Name Chemical Name R1 R2 G Q m p q (° C.) Mn (Da) HLB Supplier 1 Brij ® 56 poly(ethyleneglycol) C16H33 H O O — zero zero 32-34 683 12.9 ICl Americas, cetyl ether Norwich, NY 2 Brij ® 58 poly(ethyleneglycol) C16H33 H O O — zero zero 38-43 1124 15.7 ICl Americas, cetyl ether Norwich, NY 3 Brij ® 76 poly(ethyleneglycol) C18H37 H O O — zero zero 37-39 711 12.4 ICl Americas, stearyl ether Norwich, NY 4 Brij ® 78 poly(ethyleneglycol) C18H37 H O O — zero zero 44-46 1152 15.3 ICl Americas, stearyl ether Norwich, NY 5 Brij ® 700 poly(ethyleneglycol) C18H37 H O O — zero zero 51-54 4670 18.8 ICl Americas, stearyl ether Norwich, NY 6 — Poly(ethylene glycol) C17H35CO OCC17H35 O O — 2 2 35-37 930 — Aldrich Chemical, disterate Milwaukee, WI 7 — Poly(ethylene glycol) C17H35CO OCC17H35 O O — 2 2 52-57 12500 — Polysciences, disterate Warrington, PA 8 — Poly(ethylene glycol) bis(3- H2N(CH2)3 H2N(CH2)3 O single ~34 zero zero 49 — — Aldrich Chemical, aminopropyl) ether bond Milwaukee, WI 9 — Poly(ethylene glycol) HO2CCH2 CH2CO2H O single — zero zero — 600 — Aldrich Chemical, bis(carboxymethyl) ether bond Milwaukee, WI 10 — Poly(ethylene glycol) CH3 H O O — zero zero 20 550 — Aldrich Chemical, methyl ether Milwaukee, WI 11 — Poly(ethylene glycol) CH3 H O O — zero zero 30 750 — Aldrich Chemical, methyl ether Milwaukee, WI 12 — Poly(ethylene glycol) CH3 H O O — zero zero 52 2000 — Aldrich Chemical, methyl ether Milwaukee, WI 13 — Poly(ethylene glycol) CH3 H O O — zero zero 59 5000 — Aldrich Chemical, methyl ether Milwaukee, WI 14 — Poly(ethylene glycol) CH3 CH3 O O — zero zero 42 1000 — Aldrich Chemical, methyl ether Milwaukee, WI - Exemplary derivatives of PEG can include those shown in the Table 2 below:
-
TABLE 2 Derivatives of PEG* Average Melting Trade name Molecular Weight Pt (° C.) HLB Pluronic ® F38 4700 48 >24 Pluronic ® F77 6600 48 >24 Pluronic ® F87 7700 49 >24 Pluronic ® F68 8400 52 >24 Pluronic ® F88 11400 54 >24 Pluronic ® F127 12600 56 18-23 Pluronic ® F108 14600 57 >24 Pluronic ® F98 13000 58 >24 *Triblock copolymers of PEO and PPO (BASF, Mount Olive, NJ) - The fluid processing device can comprise a plurality of fluid flow modulators, wherein each fluid flow modulator comprises at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof. Each of the plurality of fluid flow modulators can be adapted to dissolve when contacted with water at room temperature.
- According to various embodiments, a barrier or fluid flow modulator can comprise a material having the formula:
-
R1-Q-(-CH2—)p—(—OCH2CH2—)m—(—CH2-)q-G-R2 Formula 1 - wherein:
- G and Q are each independently a single bond, O, N,
- R1 and R2 are each independently H, OH, NH2, O(CnH2n+1), O (CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1;
- R9, R10, R11, R12, R13, and R14, are each independently O, S, or NH;
- p and q are each independently 0, 1, 2; or 3,
- m is an integer from 0 to about 10,000;
- at least one of p, q, and m is an integer greater than 0;
- g is an integer from 2 to about 20; and
- n is an integer from 1 to about 20. The barrier or fluid flow modulator can comprise a material having the formula:
- wherein:
- R4, R5, and R6 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1;
- u is an integer from 0 to about 10,000;
- g is an integer from 2 to about 20;
- n is an integer from 1 to about 20;
- t, v, and z are each independently an integer from 0 to about 10,000; and
- at least one of t, u, and v, is an integer greater than 0. The barrier or fluid flow modulator can comprise a material having the formula:
-
[R7—(—CH2CH2O—)x—(—CH2CH2—)r-]a-A-R3—B—[—(—CH2CH2—)s—(—CH2CH2O—)y—R8]b Formula 3 - wherein:
- A and B are each independently a single bond, O, N,
- R7 and R8 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1;
- R3 is CnH2n, CnH2n+2, or CH2CH(CH3)O;
- R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH;
- a, b, r, and s are each independently 0, 1, 2; or 3,
- x and y are each independently an integer from 1 to about 10,000;
- g is an integer from 2 to about 20; and
- n is an integer from 1 to about 20. The barrier or fluid flow modulator can comprise a material having the formula:
- wherein:
- A, G, and Q are each independently a single bond, O, N,
- R1, R2, R4, and R5 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, CnH2n+1, (CnH2n+1)(CN)2C, or SO4H;
- R9, R10, R11, R12, R13, and R14, are each independently O, S, or NH;
- f is an integer from 1 to about 10,000;
- p and q are each independently 0, 1, 2, or 3;
- m is an integer from 0 to about 10,000;
- at least one of p, q, and m is an integer greater than 0;
- g is an integer from 2 to about 20; and
- n is an integer from 1 to about 20.
- According to various embodiments, a fluid processing device can comprise a substrate, a plurality of retainment regions formed in or on the substrate, and a barrier at least partially separating a first retainment region from a second retainment region. The barrier can comprise at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof, as described above with reference to the fluid flow modulator. The barrier can be adapted to dissolve when contacted with water at room temperature. The barrier can be included in a device as described above with reference to devices including a fluid flow modulator, in place of, or in addition to one or more fluid flow modulators. The barrier can be in the form of a fluid flow modulator.
- According to various embodiments, a method is provided that comprises processing a fluid processing device that comprises at least a first retainment region and a second retainment region, and a barrier arranged between them. At least one of the first and second retainment regions retains an aqueous solution. The barrier can comprise at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof. The barrier is adapted to dissolve when contacted with the aqueous solution. According to various embodiments, the method includes contacting the barrier with the aqueous solution to dissolve at least a portion of the barrier and form, or increase the size of, a fluid communication between the first retainment region and the second retainment region. The fluid processing device can comprise at least one additional retainment region, at least one fluid processing passageway, and at least one pressure-actuatable valve arranged in the at least one fluid processing passageway. The at least one fluid processing passageway can be in fluid communication with the at least one additional retainment region and at least one of the first retainment region and the second retainment region. The method can comprise opening the pressure-actuatable valve. The pressure-actuatable valve can comprise a diaphragm and the method can comprise bursting the diaphragm by applying pressure to the diaphragm. A heat-actuatable valve can be actuated instead of, or in addition to, actuation of a pressure-actuatable valve.
- According to various embodiments, the method can comprise migrating charged components in a sample from at least one of the at least two retainment regions, through the fluid processing passageway, by electrokinetic motion. Migration of the charged components can be accomplished by creating an electric field in the device. A system can be provided that includes an electric field generator.
- According to various embodiments, a method can comprise creating a pressure differential between a first retainment region and a second retainment region, and moving, with the pressure differential, a fluid from one of the first retainment region and the second retainment region into the other of the first retainment region and the second retainment region. The pressure differential can be generated by activating a pump. The pressure differential can comprise a positive-pressure differential or negative-pressure differential. A positive pressure means a pressure at or greater than atmospheric pressure, i.e., 1 atm. A negative pressure means a pressure less than atmospheric pressure, i.e. less than 1 atm.
- According to various embodiments, the method can comprise creating a magnetic field across a first retainment region and a second retainment region, and moving, with the magnetic field, magnetically attractable materials from one of the retainment regions toward the other retainment region.
- According to various embodiments, the method can comprise performing a set of predetermined assays in a plurality of retainment regions, for example, retainment regions, in a closed, disposable device. An exemplary device, is a cuvette. The retainment regions can be interconnected by fluid processing passageways but closed to fluid flow to or from locations outside of the cuvette. The first retainment regions can be selectively closed-off from fluid communication with second retainment regions through first channels that interconnect them. Selective closing-off can be provided by pressure-actuated valves positioned in the first channels. The second retainment regions can be interconnected to third retainment regions by second channels. Flow through the second channels can be controlled by fluid flow modulators positioned in the second channels, which can also provide selective closing-off. The method can comprise applying pressure to a pressure-actuated valve in a first channel sufficient to break the valve and provide fluid communication between the first and second retainment regions. Such a method can be used to introduce a sample for testing or other processing into one or more third retainment regions and/or establishing fluid communication between the second retainment regions and one or more third retainment regions, at a controlled rate. The controlled rate can be a function of characteristics of at least one of, a fluid in a third retainment region and a fluid within the second retainment regions.
- According to various embodiments, a system is provided that comprises a fluid processing device as described herein, and a pump, wherein the pump is arranged in fluid communication with at least one of a fluid processing passageway and one or more retainment regions.
- A system can be provided that comprises a fluid processing device as described herein, a power source, and at least two electrical leads forming electrical connections, respectively, between the power source and the at least two electrodes. A system can be provided that comprises a fluid processing device as described herein, and a magnet, wherein the magnet generates a magnetic field and the fluid processing device is arranged at least partially within the magnetic field.
- Exemplary devices and methods according to various embodiments are described below with reference to the drawings. The present teachings are not limited to the embodiments depicted in the drawings.
- Referring to
FIGS. 1( a)-1(c), a schematic illustration of the process by which a solute bridge valve establishes fluid communication between two passageways, according to various embodiments, is shown.FIG. 1( a) shows tworetainment regions fluids retainment regions fluid processing passageway 26. Asolute bridge valve 22 can consist of a plug of material that completely or partially blocks the flowfluid processing passageway 26 and separates the fluids, e.g. solvents, reagents or other materials, in therespective retainment regions fluids -
FIG. 1( b) shows the size of thesolute bridge valve 22 decreasing as the material that makes up the solute bridge valve gradually dissolves into one or both of thefluids retainment regions FIG. 1( c) shows that thefluids solute bridge valve 22 has completely dissolved. The time required to completely dissolve thesolute bridge valve 22 can be determined by the cross-sectional area and/or shape of thecapillary flow passage 26 and the length and dissolvability of thesolute bridge valve 22. By controlling the material and/or size of thesolute bridge valve 22, it is also possible to control the length of the time elapsed before the solute bridge valve has completely dissolved to allow mixing of thefluids - It is desirable for the material that makes up the
solute bridge valve 22 to be a material that dissolves into thefluids solute bridge valve 22 include polyethyleneglycol (PEG) and derivatives of polyethyleneglycol, together referred to herein as PEG. PEG has desirable properties and some PEG materials can dissolve in aqueous liquids, such as those typically used in many biological assays. PEG is generally inert and generally does not affect biological assays. PEG is easy to pattern using microfabrication techniques. PEG can be formulated that melts at relatively low temperatures, i.e. 35-50° C., and can be used as a thermal “wax.” PEG solutions are known to prevent non-specific binding and precipitation of proteins and peptides on walls of the fluid processing passageways. PEG is hygroscopic and stabilizes proteins in solutions. - According to various embodiments, the
solute bridge valve 22 can be made from a material that partially or completely separates theretainment regions fluid processing passageway 26 can be affected by the change in the open cross-sectional area of the fluid processing passageway between the two retainment regions, subsequent to the change in volume of the material. The actuation of thesolute bridge valve 22 can comprise the volumetric change of the material resulting from contact with the solution or solutions in theretainment regions solute bridge valve 22 completely blocks thefluid processing passageway 26, the tworetainment regions -
FIG. 2 shows an embodiment of an assay device that uses capillary driven flow and solute bridge valves to control a specific sequence of fluid actuation steps. The device shown inFIG. 2 can be constructed as a microfluidic chip manufactured using microfabrication techniques. According to various embodiments, the chip can comprise a set of fluid retainment regions and microchannels for housing different liquids such as reagents, samples, etc., and for mixing and reacting the various liquids.FIG. 2 shows a device according to various embodiments having a samplefluid retainment region 90 connected through afluid flow passage 80 to areaction retainment region 48. An intermediate retainment region or retainment regions is/are connected through afluid processing passageway 170 containing avalve 70 to thereaction retainment region 48. A secondintermediate retainment region 46 is connected through afluid processing passageway 172 containing avalve 72 to thereaction retainment region 48. A firstreagent retainment region 40 for containingunreacted reagents 120 can be connected through afluid flow passage 160 containing avalve 60 to oneintermediate retainment region 44, while a secondreagent retainment region 42 containingunreacted reagents 124 can be connected through afluid flow passage 162 containing avalve 62 to the secondintermediate retainment region 46. Thereaction retainment region 48 is connected through afluid processing passageway 174 containing avalve 74 to a firstwaste retainment region 50. Thewaste retainment region 50 can also be connected, through anotherfluid processing passageway 176 containing avalve 76, to a secondwaste retainment region 52. -
Reagent retainment regions intermediate retainment regions valves fluid flow passages valves fluid processing passageways reagent retainment regions - The
intermediate retainment regions fluid flow passages valves reaction retainment region 48. Fluid communication through thefluid flow passages valves passages solute bridge valves fluid flow passages solute bridge valves fluid flow passages intermediate retainment regions reaction retainment region 48 as a result of their responsiveness to stimuli such as the chemical composition of the fluids withinretainment regions reaction retainment region 48. Each ofregions vent -
FIG. 3 illustrates an embodiment wherein the retainment regions and fluid processing passageways are arranged such thatsolute bridge valves bridge valves FIG. 2 , are arranged in a line for ease of manufacture. Each ofregions vent -
FIGS. 4A-4J illustrate a sequence of events that can occur during operation of a diagnostic device according to various embodiments, such as exemplified inFIG. 2 .FIGS. 4A-4J are explained in greater detail below. Each ofregions vent -
FIGS. 5A-5J illustrate a sequence of events that can occur during operation of a diagnostic device according to various embodiments, such as exemplified inFIG. 3 .FIGS. 5A-5J are explained in greater detail below. Each ofregions vent - In
FIG. 4A a diagnostic device according to various embodiments is provided withretainment regions wash buffer 124 inretainment region 42 and adetection reagent 120 withinretainment region 40. Pressure actuatedvalves fluid flow passages retainment regions intermediate retainment regions - In
FIG. 4B a user can apply pressure to theretainment regions valves fluid flow passages valves retainment regions intermediate retainment regions - As shown in
FIG. 4C a user can then inject asample 126 into thesample retainment region 90, which is connected to thereaction retainment region 48 through afluid flow passage 80. Sample injection could alternatively occur before or at the same time as bursting the valves.Fluid flow passages intermediate retainment regions reaction retainment region 48, as well as the fluid communication between thesample retainment region 90 and thereaction retainment region 48. Fluid flow of the sample fromsample retainment region 90 intoreaction retainment region 48 can also be automatically controlled as a result of the dimensions of thefluid flow passage 80. For example, thefluid flow passage 80 can be provided as a capillary passage such that the sample material fromsample retainment region 90 gradually wicks into thereaction retainment region 48, without the need for a solute bridge valve to control this flow throughfluid flow passage 80. Pressure can be relieved or equalized viavent 91 and/or 49. - As shown in
FIG. 4D , the sample material that is now inreaction retainment region 48 contacts thesolute bridge valve 72 on one side of thevalve 72 influid flow passage 172, while the reagent inintermediate retainment region 46 contacts thesolute bridge valve 72 from the opposite side of the valve. One or more of the reagent inretainment region 46 and/or the sample inreaction retainment region 48 begin to dissolve or otherwise affect the volume of the material making up thesolute bridge valve 72. After a certain amount of time that is automatically controlled by at least one of the flow cross-section ofpassage 172, or the volume or composition of material at least partially making-up thesolute bridge valve 72, thesolute bridge valve 72 no longer prevents the reagent inretainment region 46 from gradually diffusing into thesample 126 inreaction retainment region 48, as shown inFIG. 4E . Pressure can be relieved and/or equalized viavent 43. - The
flow passage 174 leading from thereaction retainment region 48 intowaste retainment region 50 can also be provided with dimensions that allow for capillary action, and asolute bridge valve 74 that will gradually dissolve or otherwise change volume as a result of contact with the fluid fromreaction retainment region 48. As shown inFIG. 4F , the effect of the fluid withinreaction retainment region 48 on thesolute bridge valve 74 withinflow passage 174 gradually opens theflow passage 174 within which thevalve 74 is positioned to allow fluid communication between thereaction retainment region 48 and the firstwaste retainment region 50. The flow of fluid fromreaction region 48 intowaste region 50 throughfluid processing passageway 174 contributes to a capillary flow of more reagent fromreagent region 42 throughfluid processing passageway 162 andintermediate region 46 intoreaction region 48. Pressure resulting from such flow can be relieved viavent 43 and/or vent 49. Flow of fluid fromreaction region 48 intowaste region 50, as shown inFIG. 4G , can also cause more ofsample 126 to flow fromsample region 90 intoreaction region 48. Pressure resulting from the flow of fluid fromreaction region 48 intowaste region 50, can be relieved viavent 49 and/or vent 51. According to various embodiments, the relative dimensions of the flow passages such asflow passage 80 leading fromsample retainment region 90 intoreaction retainment region 48, and theflow passage 172 within whichvalve 72 is positioned leading fromintermediate retainment region 46 intoreaction retainment region 48, can be selected in order to contribute to a preferential flow of fluid from theintermediate retainment region 46 intoreaction retainment region 48. A smaller flow cross-section throughpassage 80 than the flow cross-section throughpassage 172 would result in more fluid flowing from thereagent retainment region 42 andintermediate retainment region 46 intoreaction retainment region 48 than the amount of sample flowing fromsample retainment region 90 into thereaction retainment region 48. - After a predetermined amount of time,
solute bridge valve 70 provided in theflow passage 170 between intermediateretainment region 44 andreaction retainment region 48 can also begin to dissolve, melt, or otherwise change in volume such thatreagent 120 flows fromreagent retainment region 40 throughintermediate retainment region 44 and into thereaction retainment region 48, as shown inFIG. 4H . The relative cross-sectional flow areas of the various flow passages connecting retainment regions as well as the amount of material provided in the solute bridge valves within the flow passages can be varied in order to control the amount of time it takes for the reagents and other fluids within the retainment regions to move from one retainment region to the next, thereby providing a control of the fluid handling steps. - After more time has passed,
solute bridge valve 76 inflow passage 176 leading to a secondwaste retainment region 52 can begin to dissolve, melt, or otherwise change in volume such that fluid can flow fromwaste retainment region 50 into secondwaste retainment region 52, as shown inFIGS. 4I and 4J . This flow can cause more of the reagents and sample to flow fromregions reaction region 48. Pressure can be relieved viavent 49 and/or vent 91. - In an alternative embodiment, as exemplified in
FIG. 3 andFIGS. 5A-5J , the diagnostic device can comprise a set of retainment regions and microchannels corresponding to the retainment regions and microchannels of the embodiment exemplified in FIGS. 2 and 4A-4J, but with thesolute bridge valves retainment regions sample retainment region 90 a is controlled automatically corresponds with the process described above for the embodiment shown inFIG. 2 andFIGS. 4A-4J . - A sample solution can be added to
sample retainment region 90 a, and supplied to areaction retainment region 48 a through acapillary flow passage 80 a, as shown inFIGS. 5C and 5D . Solutions, such as reagents and/or wash buffers, can be dispensed fromretainment regions valves passageways intermediate retainment regions intermediate retainment region 46 a then begins to act onsolute bridge valve 72 a influid flow passage 172 a, opening up a passageway for the solution to enterpassage 80 a andreaction retainment region 48 a, as shown inFIGS. 5D , 5E, 5F and 50.FIG. 5H illustrates solution fromretainment region 44 a dissolving, or otherwise reducing the volume ofsolute bridge valve 70 a inpassageway 170 a.Solute bridge valve 74 a inpassageway 174 a has also dissolved inFIG. 5H to allow solution from thereaction retainment region 48 a to pass to awaste retainment region 50 a. As described above with regard to the embodiment ofFIG. 2 , the flow of solution fromreaction region 48 a to wasteregion 50 a can create a suction that can draw more solution fromregion 46 a throughfluid processing passageway 172 a and capillaryfluid processing passageway 80 a into thereaction region 48 a, as shown inFIG. 5I .Solute bridge valve 76 a influid processing passageway 176 a then dissolves, allowing solution to flow to wasteregion 52 a, and can create suction that can draw solution fromregion 44 a intoreaction region 48 a, as well as drawing additional solution fromregion 46 a and additional sample fromregion 90 a, as shown inFIG. 5J . For example, vents 41 and 43 are needed in 40 and 42, respectively, (FIG. 4A ) in order to allow 120 and 124 to flow into 44 and 46, respectively. Likewise, vent 91 is needed in communication withregion 90. Avent 49 can be provided in communication with regions 48 (FIG. 4D ) such that 120, 124 and 126 can flow into it. Without a vent, the air trapped in 48 can prevent any inflow of liquid. The same can apply toregions - The arrangement of retainment regions, passageways and valves of the various embodiments exemplified in
FIG. 3 provides for ease of manufacturing. As shown inFIG. 3 andFIGS. 5A-5J , thesolute bridge valves diagnostic device 130 shown inFIG. 6 exemplifies an embodiment wherein the solute bridge valves are formed as one extended length of solutebridge valve material 270 in asubstrate 134 separate from asubstrate 132, within which various retainment regions such asretainment regions substrates device 130, the length of solutebridge valve material 270 can connect topassageways FIG. 7 , which passageways are connected to various retainment regions. As illustrated inFIG. 7 , even if the twosubstrates bridge valve material 270 will still connect with thepassageways FIG. 8 illustrates a situation wherein separate substrates forsolute bridge valves passageways FIG. 2 . In this situation thesolute bridge valves passageways - Referring to
FIG. 9 , and according to various embodiments,retainment regions substrate 440, withretainment region 444 interconnected withretainment region 448 through apassageway 470, andretainment region 446 interconnected withretainment region 448 through apassageway 472. Solution such as reagents and/or wash buffers can be retained in theretainment regions material 460 applied over the top surface ofsubstrate 440 and adhered to the top surface by anadhesive layer 462. Pressure actuatedvalves passageways retainment regions flexible sheet 460 over the respective retainment regions will dispense the solutions throughpassages retainment region 448. Abarrier 450 can define aninner retainment region 430 and provide an automatically controlled interaction between the solution orsolutions 442 in theretainment region 448 and a solution retained in theinner retainment region 430. If desired, thebarrier 450 can be formed from a solute material such as PEG that will gradually dissolve and thereby control the interaction between the solution orsolutions 442 inouter retainment region 448 and a solution retained in theinner retainment region 430. - According to various embodiments, and as exemplified by the embodiment shown in
FIG. 10 , a device can be provided that comprises asubstrate 540 having aretainment region 542 formed in the substrate and covered by asheet 560 that is adhered to the top surface of thesubstrate 540 by an adhesive 562. Aninner retainment region 530 can be defined within theretainment region 542 by abarrier 550 that can act as a fluid flow modulator between a solution in theouter retainment region 542 and a solution or material in theinner retainment region 530. Thebarrier 550 can comprise aportion 552 made from a soluble material, and aportion 554 made from an insoluble material to provide a further degree of automatic control of the interaction between the solutions or other ingredients inretainment regions retainment regions 542 to initiate a process. A septum (not shown) can be provided as an injection port. - According to various embodiments, and as exemplified in the embodiment shown in
FIG. 11 , thesubstrate 700 can be provided with aretainment region 740 connected through apassage 770 having a pressure actuatedvalve 772 to asecond retainment region 742. Similarly, anotherretainment region 740 a can be connected through a passage 770 a having a pressure actuatedvalve 772 a to thesecond retainment region 742. A star-shaped or otherwisepolygonal retainment region 730 can be defined inside of thesecond retainment region 742 by abarrier 750. All of the retainment regions can be covered by asheet 760 adhered to the top surface ofsubstrate 700 by anadhesive layer 762. A solution withinretainment region 740 can be dispensed throughpassage 770 by applying pressure to thesheet 760 over theretainment region 740 to force the liquid past the pressure actuatedvalve 772 into thesecond retainment region 742. Thebarrier 750 can comprise a material such as PEG that gradually dissolves or melts in response to a stimuli such as characteristics of the solution that has been introduced to thesecond retainment region 742, thereby providing an automatically controlled interaction between the solution insecond retainment region 742 and the solution inretainment region 730. In an exemplary device, fluid processing passageways could interconnect with each respective point of the star shape shown. - According to various embodiments, further control of the fluid handling steps can be provided by including various solute structures within the fluid processing passageways and/or the retainment regions. The solute structures can be selected to dissolve over a finite amount of time and change the flow properties of the fluidic circuit. As an example, raised structures (such as pillars of different aspect ratios) made from solute material (such as PEG) can be fabricated by photolithography inside the various retainment regions, retainment regions, and/or fluid processing passageways. The incorporation of these structures can cause the flow paths to have different capillarity and can cause capillary suction pressures of different magnitudes in different parts of the fluidic circuit. The structures can also introduce additional flow resistance, with a variation in the flow resistance depending on the dissolution of the solute structures.
- In one example, an array of pillars made of PEG could be fabricated inside of the
waste retainment regions FIG. 2 , or 50 a, 52 a in the embodiment ofFIG. 3 , which could, for example, cause higher suction pressure inwaste retainment region reaction retainment region waste retainment region retainment region fluid processing passageway 176 betweenwaste retainment region 50 andwaste retainment region 52, for example, could then result in the liquid inwaste retainment region 50 being pulled intowaste retainment region 52. The pulling can be as a result of a larger capillary suction pressure inwaste retainment region 52 caused by solute structures inretainment region 52. The PEG cannot operate until 74 and 76 inFIG. 2 and 170 a, 172 a and 174 a inFIG. 3 are open. The flow of 120, 125 and 126 inFIG. 2 into 44, 46, and 48, respectively, relies on capillary effect alone, and does not rely on vacuum created by the PEG in 50 or 52 because neither 76 nor 74 are open. PEG can facilitate fluid flow from 48 into 50 and/or 52 without a vent. The flow of 120, 124 or 126 into 44, 46, or 48, respectively, relies on capillary effect that requires air vents to prevent pressure build up. - Those skilled in the art can appreciate from the foregoing description that the present teachings can be implemented in a variety of forms. Therefore, while these teachings have been described in connection with particular embodiments and examples thereof, the true scope of the present teachings should not be so limited. Various changes and modifications can be made without departing from the scope of the teachings herein.
Claims (13)
1. A fluid processing device comprising:
a fluid processing passageway;
a plurality of retainment regions, at least two of the retainment regions each being in fluid communication with the fluid processing passageway; and
at least one fluid flow modulator arranged in the fluid processing passageway and being adapted to open to form, or to increase in size, a fluid communication between the at least two retainment regions, the fluid flow modulator comprising at least one of a polyethylene glycol material, a derivative of a polyethylene glycol material, and a combination thereof, and being adapted to dissolve when contacted with water at room temperature.
2. The fluid processing device of claim 1 , wherein at least one of the plurality of retainment regions comprises aqueous fluid retained therein.
3. The fluid processing device of claim 1 , wherein the at least one fluid flow modulator comprises at least one of a polyethylene glycol material and a derivative of a polyethylene glycol material, having a melting point of from about 35° C. and about 65° C.
4. The fluid processing device of claim 1 , wherein the fluid processing passageway is dimensioned sufficient to cause capillary flow of a fluid from at least one of the at least two retainment regions through the fluid processing passageway.
5. The fluid processing device of claim 1 , wherein the fluid processing passageway is dimensioned sufficient to cause electrokinetic migration of charged components in a fluid, from at least one of the at least two retainment regions through the fluid processing passageway
6. The fluid processing device of claim 5 , further comprising at least two electrodes disposed in the device with the fluid processing passageway therebetween.
7. The fluid processing device of claim 1 , further comprising:
at least one additional retainment region;
at least one additional fluid processing passageway; and
at least one valve comprising one or more of a pressure-actuatable valve and a heat-actuatable valve arranged in the at least one additional fluid processing passageway, wherein the at least one additional fluid processing passageway is in fluid communication with the at least one additional retainment region and at least one of the plurality of retainment regions.
8. A system comprising the fluid processing device of claim 1 , and a pump, wherein the pump is arranged in fluid communication with at least one of the fluid processing passageway and one or more of the plurality of retainment regions.
9. A system comprising the fluid processing device of claim 6 , a power source, and at least two electrical leads forming electrical connections, respectively, between the power source and the at least two electrodes.
10. The fluid processing device of claim 1 , wherein the at least one fluid flow modulator comprises a material having the formula:
R1-Q-(-CH2—)p—(—OCH2CH2—)m—(—CH2-)q-G-R2 Formula I
R1-Q-(-CH2—)p—(—OCH2CH2—)m—(—CH2-)q-G-R2 Formula I
wherein
G and Q are each independently a single bond, O, N
R1 and R2 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
R9, R10, R11, R12, R13, and R14, are each independently O, S, or NH,
p and q are each independently 0, 1, 2, or 3,
m is an integer from 0 to about 10,00,
at least one of p, q, and m is an integer greater than 0,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20;
wherein
u is an integer from 0 to about 10,000,
g is an integer from 2 to about 20,n is an integer from 1 to about 20,
t, v, and z are each independently an integer from 0 to about 10,000, and
at least one oft, u, and v, is an integer greater than 0;
R4, R5, and R6are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n+1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO3H, CgH2g−1, or CnH2n+1,
u is an integer from 0 to about 10,000,
g is an integer from 2 to about 20,
n is an integer from 1 to about 20,
t, v, and z are each independently an integer from 0 to about 10,000, and
at least one oft, u, and v, is an integer greater than 0;
[R7—(—CH2CH2O—)x—(—CH2CH2—)r-]a-A-R3—B—[—(—CH2CH2—)s—(—CH2CH2O—)y—R8]b Formula 3
[R7—(—CH2CH2O—)x—(—CH2CH2—)r-]a-A-R3—B—[—(—CH2CH2—)s—(—CH2CH2O—)y—R8]b Formula 3
wherein
A and B are each independently a single bond, O, N,
R7 and R8 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1); CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
R3 is CnH2n, CnH2n−2, or CH2CH(CH3)O,
R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH,
a, b, r, and s are independently 0, 1, 2, or 3,
x and y are each independently an integer from 1 to about 10,000,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20; or
R1, R2, R4, and R5 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH,
f is an integer from 1 to about 10,000,
p and q are each independently 0, 1, 2, or 3,
m is an integer from 0 to about 10,000,
at least one of p, q, and m is an integer greater than 0,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20.
11. A fluid processing device comprising:
a fluid processing passageway; and
at least one fluid flow modulator arranged in the fluid processing passageway and being adapted to open to form, or increase the size of, a fluid communication through the fluid processing passageway, the at least one fluid flow modulator being adapted to dissolve when contacted with water at room temperature, and having the formula:
R1-Q-(-CH2—)p—(—OCH2CH2—)m—(—CH2-)q-G-R2 Formula I
R1-Q-(-CH2—)p—(—OCH2CH2—)m—(—CH2-)q-G-R2 Formula I
wherein
G and Q are each independently a single bond, O, N,
R1 and R2 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH,
p and q are each independently 0, 1, 2, or 3,
m is an integer from 0 to about 10,000,
at least one of p, q, and m is an integer greater than 0,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20;
wherein
R4, R5, and R6 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
u is an integer from 0 to about 10,000,
g is an integer from 2 to about 20,
n is an integer from 1 to about 20,
t, v, and z are each independently an integer from 0 to about 10,000, and
at least one oft, u, and v, is an integer greater than 0;
[R7—(—CH2CH2O—)x—(—CH2CH2-)r-]a-A-Rj-B—[—(—CH2CH2—)s—(—CH2CH2O—)y—R8]b Formula 3
[R7—(—CH2CH2O—)x—(—CH2CH2-)r-]a-A-Rj-B—[—(—CH2CH2—)s—(—CH2CH2O—)y—R8]b Formula 3
wherein
A and B are each independently a single bond, O, N,
R7 and R8 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n−1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, or CnH2n+1,
R3 is CnH2n, CnH2n−2, or CH2CH(CH3)O,
R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH,
a, b, r, and s are independently 0, 1, 2, or 3,
x and y are each independently an integer from 1 to about 10,000,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20; or
R1, R2, R4, and R5 are each independently H, OH, NH2, O(CnH2n+1), O(CnH2n'1 1), CH2OH, —(—CH2CH2O—)n—H, CH2CH2CH2NH2, CH2CO2H, CgH2g−1, CnH2n+1, (CnH2n+1)(CN)2C, or SO4H;
R9, R10, R11, R12, R13, and R14, can each independently be O, S, or NH,
f is an integer from 1 to about 10,000,
p and q are each independently 0, 1, 2, or 3,
m is an integer from 0 to about 10,000,
at least one of p, q, and m is an integer greater than 0,
g is an integer from 2 to about 20, and
n is an integer from 1 to about 20.
12-19. (canceled)
20. A method of performing a set of predetermined assays, comprising:
providing a plurality of retainment regions in a closed, disposable cuvette, the retainment regions interconnected by fluid processing passageways but closed to fluid flow to or from locations outside of the cuvette, the cuvette including:
first retainment regions;
second retainment regions;
first fluid processing passageways interconnecting the first retainment regions from the second retainment regions, wherein the first retainment regions are selectively closed-off from fluid communication with the second retainment regions by pressure-actuated valves positioned in the first fluid processing passageways;
one or more third retainment regions interconnected by second fluid processing passageways with at least the second retainment regions; and
fluid flow modulators positioned in the second fluid processing passageways;
applying pressure to the pressure actuated valves in the first fluid processing passageways sufficient to provide fluid communication between the first and second retainment regions;
introducing a sample for testing or other processing into the one or more third retainment regions; and
establishing fluid communication between the second retainment regions and the one or more third retainment regions at a controlled rate that is a function of characteristics of at least one of the sample in the one or more third retainment regions and a fluid within the second retainment regions.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/954,519 US8147770B2 (en) | 2004-10-18 | 2010-11-24 | Microfluidic device including a dissolvable structure for flow control |
US13/403,355 US20120214156A1 (en) | 2004-10-18 | 2012-02-23 | Device including a dissolvable structure for flow control |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61962304P | 2004-10-18 | 2004-10-18 | |
US61973104P | 2004-10-18 | 2004-10-18 | |
US61967704P | 2004-10-18 | 2004-10-18 | |
US11/252,821 US20060093528A1 (en) | 2004-10-18 | 2005-10-18 | Device including a dissolvable structure for flow control |
US12/627,799 US20100136701A1 (en) | 2004-10-18 | 2009-11-30 | Device including a dissolvable structure for flow control |
US12/954,519 US8147770B2 (en) | 2004-10-18 | 2010-11-24 | Microfluidic device including a dissolvable structure for flow control |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/627,799 Continuation US20100136701A1 (en) | 2004-10-18 | 2009-11-30 | Device including a dissolvable structure for flow control |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/403,355 Continuation US20120214156A1 (en) | 2004-10-18 | 2012-02-23 | Device including a dissolvable structure for flow control |
Publications (2)
Publication Number | Publication Date |
---|---|
US20110126913A1 true US20110126913A1 (en) | 2011-06-02 |
US8147770B2 US8147770B2 (en) | 2012-04-03 |
Family
ID=36203645
Family Applications (9)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/252,821 Abandoned US20060093528A1 (en) | 2004-10-18 | 2005-10-18 | Device including a dissolvable structure for flow control |
US11/252,914 Active 2029-10-17 US8312890B1 (en) | 2004-10-18 | 2005-10-18 | Dissolvable valve in a fluid processing device |
US11/252,912 Abandoned US20060090800A1 (en) | 2004-10-18 | 2005-10-18 | Fluid processing device including size-changing barrier |
US11/252,915 Active 2028-10-28 US8062611B2 (en) | 2004-10-18 | 2005-10-18 | Fluid processing device including composite material flow modulator |
US12/627,824 Abandoned US20100133104A1 (en) | 2004-10-18 | 2009-11-30 | Fluid processing device including size-changing barrier |
US12/627,799 Abandoned US20100136701A1 (en) | 2004-10-18 | 2009-11-30 | Device including a dissolvable structure for flow control |
US12/945,793 Active US8383062B2 (en) | 2004-10-18 | 2010-11-12 | Fluid processing device including size-changing barrier |
US12/954,519 Active US8147770B2 (en) | 2004-10-18 | 2010-11-24 | Microfluidic device including a dissolvable structure for flow control |
US13/403,355 Abandoned US20120214156A1 (en) | 2004-10-18 | 2012-02-23 | Device including a dissolvable structure for flow control |
Family Applications Before (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/252,821 Abandoned US20060093528A1 (en) | 2004-10-18 | 2005-10-18 | Device including a dissolvable structure for flow control |
US11/252,914 Active 2029-10-17 US8312890B1 (en) | 2004-10-18 | 2005-10-18 | Dissolvable valve in a fluid processing device |
US11/252,912 Abandoned US20060090800A1 (en) | 2004-10-18 | 2005-10-18 | Fluid processing device including size-changing barrier |
US11/252,915 Active 2028-10-28 US8062611B2 (en) | 2004-10-18 | 2005-10-18 | Fluid processing device including composite material flow modulator |
US12/627,824 Abandoned US20100133104A1 (en) | 2004-10-18 | 2009-11-30 | Fluid processing device including size-changing barrier |
US12/627,799 Abandoned US20100136701A1 (en) | 2004-10-18 | 2009-11-30 | Device including a dissolvable structure for flow control |
US12/945,793 Active US8383062B2 (en) | 2004-10-18 | 2010-11-12 | Fluid processing device including size-changing barrier |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/403,355 Abandoned US20120214156A1 (en) | 2004-10-18 | 2012-02-23 | Device including a dissolvable structure for flow control |
Country Status (3)
Country | Link |
---|---|
US (9) | US20060093528A1 (en) |
EP (3) | EP1824601B1 (en) |
WO (3) | WO2006044896A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060093526A1 (en) * | 2004-10-18 | 2006-05-04 | Applera Corporation | Fluid processing device including composite material flow modulator |
WO2013153181A1 (en) * | 2012-04-13 | 2013-10-17 | Technische Universität Dresden | Method and device for targeted process control in a microfluidic processor having integrated active elements |
US10258984B2 (en) | 2014-06-16 | 2019-04-16 | Koninklijke Philips N.V. | Cartridge for fast sample intake |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7722809B2 (en) * | 2004-06-04 | 2010-05-25 | Wisconsin Alumni Research Foundation | Bioagent detection device |
US20070054291A1 (en) * | 2005-05-20 | 2007-03-08 | Van Camp John R | System for detection of nucleic acids |
WO2007011867A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Fluid processing device and method |
WO2007043619A1 (en) * | 2005-10-13 | 2007-04-19 | Nissui Pharmaceutical Co., Ltd. | Testing device |
KR100738113B1 (en) * | 2006-05-10 | 2007-07-12 | 삼성전자주식회사 | Phase transition type valve and the manufacturing method thereof |
EP1878495A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Analytical device for thermally treating a fluid and/or monitoring a property thereof |
WO2008006618A1 (en) * | 2006-07-14 | 2008-01-17 | Roche Diagnostics Gmbh | Analytical device for thermally treating a fluid and/or monitoring a property thereof |
TWI404929B (en) * | 2006-07-17 | 2013-08-11 | Ind Tech Res Inst | Fluidic device and controlling method thereof |
US7794665B2 (en) * | 2006-07-17 | 2010-09-14 | Industrial Technology Research Institute | Fluidic device |
EP1920842A1 (en) * | 2006-10-27 | 2008-05-14 | Konica Minolta Medical & Graphic, Inc. | Microchip and microchip inspection system |
KR100846501B1 (en) * | 2006-11-09 | 2008-07-17 | 삼성전자주식회사 | Valve unit and fluid treating apparatus with the same |
US20080153155A1 (en) * | 2006-11-22 | 2008-06-26 | Kota Kato | Microchannel chip |
US7863035B2 (en) * | 2007-02-15 | 2011-01-04 | Osmetech Technology Inc. | Fluidics devices |
AU2008265610B2 (en) | 2007-06-21 | 2012-08-23 | Gen-Probe Incorporated | Instrument and receptacles for performing processes |
CA2668792C (en) * | 2008-06-30 | 2017-08-01 | Tyco Healthcare Group Lp | Valve assembly including a dissolvable valve member |
JP4722977B2 (en) * | 2008-08-27 | 2011-07-13 | シャープ株式会社 | Detection instrument, analyzer, detection method, and control method of detection instrument |
EP2391452B1 (en) * | 2009-01-30 | 2015-06-17 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
DE102009015395B4 (en) * | 2009-03-23 | 2022-11-24 | Thinxxs Microtechnology Gmbh | Flow cell for treating and/or examining a fluid |
US9500572B2 (en) | 2009-04-30 | 2016-11-22 | Purdue Research Foundation | Sample dispenser including an internal standard and methods of use thereof |
CN105606691B (en) * | 2009-04-30 | 2018-12-28 | 普度研究基金会 | The method for analyzing the protein or peptide in sample |
WO2011027092A1 (en) * | 2009-09-01 | 2011-03-10 | F. Hoffmann-La Roche Ag | Micro-fluidic structures |
US20120026009A1 (en) * | 2010-07-30 | 2012-02-02 | Yanzhu Zhao | Medical device having a multi-element antenna |
CN103403533B (en) | 2011-02-24 | 2017-02-15 | 简.探针公司 | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
GB2491813A (en) * | 2011-06-03 | 2012-12-19 | Univ Dublin City | A microfluidic device with sacrificial valve |
US20140093980A1 (en) * | 2012-10-01 | 2014-04-03 | University Of Washington Through Its Center For Commercialization | Dissolvable bridges for manipulating fluid volumes and associated devices, systems and methods |
KR20140055528A (en) * | 2012-10-31 | 2014-05-09 | 삼성전자주식회사 | Microfluidic structure, microfluidic system and control method for microfluidic test device |
EP2948249A1 (en) | 2013-01-22 | 2015-12-02 | University of Washington through its Center for Commercialization | Sequential delivery of fluid volumes and associated devices, systems and methods |
EP2951852B1 (en) | 2013-01-31 | 2020-07-22 | Purdue Research Foundation | Systems for analyzing an extracted sample |
WO2014120552A1 (en) | 2013-01-31 | 2014-08-07 | Purdue Research Foundation | Methods of analyzing crude oil |
WO2014181500A1 (en) * | 2013-05-08 | 2014-11-13 | ソニー株式会社 | Flow channel device, analytical apparatus, and fluid apparatus |
GB2515116A (en) * | 2013-06-14 | 2014-12-17 | Univ Dublin City | Microfluidic Device |
EP3486937B1 (en) | 2013-06-25 | 2022-07-27 | Purdue Research Foundation | Mass spectrometry analysis of microorganisms in samples |
WO2015044454A2 (en) * | 2013-09-30 | 2015-04-02 | Göran Stemme | A microfluidic device, use and methods |
US9786478B2 (en) | 2014-12-05 | 2017-10-10 | Purdue Research Foundation | Zero voltage mass spectrometry probes and systems |
WO2016127177A1 (en) | 2015-02-06 | 2016-08-11 | Purdue Reserach Foundation | Probes, systems, cartridges, and methods of use thereof |
CN107923905B (en) * | 2015-06-20 | 2020-05-26 | 卡皮泰奈尔公司 | Plasma separation microfluidic device |
US10039113B2 (en) | 2016-03-28 | 2018-07-31 | Bank Of America Corporation | Intelligent resource procurement system based on physical proximity to related resources |
TW201910741A (en) * | 2017-08-02 | 2019-03-16 | 鴻海精密工業股份有限公司 | Liquid quality detecting device having a liquid blocking function |
US10046322B1 (en) | 2018-03-22 | 2018-08-14 | Talis Biomedical Corporation | Reaction well for assay device |
WO2019232591A1 (en) * | 2018-06-08 | 2019-12-12 | The University Of Sydney | An array of reconfigurable modular units for movement of materials |
US10717080B2 (en) * | 2018-09-05 | 2020-07-21 | Paratus Diagnostics, LLC | Systems and method for metering and timing of fluid flow in a point-of-care diagnostic cartridge |
CN109505829B (en) * | 2018-11-28 | 2021-12-03 | 中国核电工程有限公司 | Passive modularized fluid resistance element |
US10820847B1 (en) | 2019-08-15 | 2020-11-03 | Talis Biomedical Corporation | Diagnostic system |
KR102544040B1 (en) * | 2021-03-05 | 2023-06-15 | 충남대학교산학협력단 | Method for Controlling Fluid Flow Using Enzyme Reaction and Diagnosis Chip Using the Method |
WO2023219148A1 (en) * | 2022-05-13 | 2023-11-16 | 株式会社Provigate | Fluid device |
Citations (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4522923A (en) * | 1983-10-03 | 1985-06-11 | Genetic Diagnostics Corporation | Self-contained assay method and kit |
US4549952A (en) * | 1982-11-22 | 1985-10-29 | Eastman Kodak Company | Capillary transport device having means for increasing the viscosity of the transported liquid |
US4800066A (en) * | 1986-07-21 | 1989-01-24 | The Drackett Company | End of life indicator for automatic toilet cleaning devices |
US5798215A (en) * | 1993-02-18 | 1998-08-25 | Biocircuits Corporation | Device for use in analyte detection assays |
US5846396A (en) * | 1994-11-10 | 1998-12-08 | Sarnoff Corporation | Liquid distribution system |
US6030581A (en) * | 1997-02-28 | 2000-02-29 | Burstein Laboratories | Laboratory in a disk |
US6102897A (en) * | 1996-11-19 | 2000-08-15 | Lang; Volker | Microvalve |
US6152181A (en) * | 1992-11-16 | 2000-11-28 | The United States Of America As Represented By The Secretary Of The Air Force | Microdevices based on surface tension and wettability that function as sensors, actuators, and other devices |
US6302134B1 (en) * | 1997-05-23 | 2001-10-16 | Tecan Boston | Device and method for using centripetal acceleration to device fluid movement on a microfluidics system |
US6375901B1 (en) * | 1998-06-29 | 2002-04-23 | Agilent Technologies, Inc. | Chemico-mechanical microvalve and devices comprising the same |
US20020121487A1 (en) * | 2001-01-03 | 2002-09-05 | Robotti Karla M. | Methods and using chemico-mechanical microvalve devices for the selective separation of components from multi-component fluid samples |
US20020143437A1 (en) * | 2001-03-28 | 2002-10-03 | Kalyan Handique | Methods and systems for control of microfluidic devices |
US20020153251A1 (en) * | 1999-02-03 | 2002-10-24 | Alexander Sassi | Multichannel control in microfluidics |
US20020194909A1 (en) * | 2000-10-24 | 2002-12-26 | Hasselbrink Ernest F. | Mobile monolithic polymer elements for flow control in microfluidic devices |
US20030019522A1 (en) * | 2001-07-26 | 2003-01-30 | Gene Parunak | Methods and systems for fluid control in microfluidic devices |
US6615855B2 (en) * | 2000-02-25 | 2003-09-09 | Science & Technology Corporation @T Unm | Stimuli-responsive hybrid materials containing molecular actuators and their applications |
US20040043507A1 (en) * | 2002-08-27 | 2004-03-04 | Kimberly-Clark Worldwide, Inc. | Fluidics-based assay devices |
US20040067168A1 (en) * | 2000-12-08 | 2004-04-08 | Frederic Buffiere | Temporary separation barrier, container comprising same and method for carrying out a test in said container |
US20040089616A1 (en) * | 1997-05-23 | 2004-05-13 | Gregory Kellogg | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
US20040096358A1 (en) * | 2002-11-14 | 2004-05-20 | Gert Blankenstein | Device for the stepwise transport of liquid utilizing capillary forces |
US20040175296A1 (en) * | 1999-11-15 | 2004-09-09 | Opalsky Cindra A. Widrig | Apparatus and method for assaying coagulation in fluid samples |
US20040208792A1 (en) * | 2002-12-20 | 2004-10-21 | John Linton | Assay apparatus and method using microfluidic arrays |
US20050244504A1 (en) * | 2003-09-23 | 2005-11-03 | Little Steven R | pH triggerable polymeric particles |
US20060090800A1 (en) * | 2004-10-18 | 2006-05-04 | Applera Corporation | Fluid processing device including size-changing barrier |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5846386A (en) * | 1994-01-07 | 1998-12-08 | Startec Ventures, Inc. | On-site ammonia purification for semiconductor manufacture |
AU2034999A (en) * | 1997-08-01 | 1999-02-22 | Aalto Scientific, Ltd. | Pipetting devices preloaded with standardized control sample materials |
GB9804483D0 (en) * | 1998-03-02 | 1998-04-29 | Central Research Lab Ltd | Apparatus for and method of controlling the rate of flow of fluid along a pathway |
JP2002527250A (en) * | 1998-10-13 | 2002-08-27 | バイオマイクロ システムズ インコーポレイテッド | Fluid circuit components based on passive hydrodynamics |
SE9902474D0 (en) | 1999-06-30 | 1999-06-30 | Amersham Pharm Biotech Ab | Polymer valves |
US6589778B1 (en) * | 1999-12-15 | 2003-07-08 | Amersham Biosciences Ab | Method and apparatus for performing biological reactions on a substrate surface |
EP1384076B1 (en) * | 2001-03-19 | 2012-07-25 | Gyros Patent Ab | Characterization of reaction variables |
WO2002088296A1 (en) * | 2001-04-26 | 2002-11-07 | Boston Biomedica, Inc. | Multichamber device and uses thereof for processing of biological samples |
CA2455894A1 (en) * | 2001-09-17 | 2003-03-27 | Gyros Ab | Functional unit enabling controlled flow in a microfluidic device |
US6616855B1 (en) * | 2001-09-27 | 2003-09-09 | Taiwan Semiconductor Manufacturing Company | Process to reduce surface roughness of low K damascene |
WO2003093802A1 (en) * | 2002-04-30 | 2003-11-13 | Gyros Ab | Integrated microfluidic device (ea) |
EP2096341A1 (en) * | 2002-12-04 | 2009-09-02 | Spinx, Inc, | Devices and methods for programmable microscale manipulation of fluids |
JP4519124B2 (en) * | 2003-01-30 | 2010-08-04 | ユィロス・パテント・アクチボラグ | Wall inside the microfluidic device |
KR100959101B1 (en) * | 2003-02-20 | 2010-05-25 | 삼성전자주식회사 | Polymerase chain reaction device and method for regulating opening or shutting of inlet and outlet of PCR device |
SE0300822D0 (en) * | 2003-03-23 | 2003-03-23 | Gyros Ab | A collection of Micro Scale Devices |
-
2005
- 2005-10-18 WO PCT/US2005/037451 patent/WO2006044896A2/en active Application Filing
- 2005-10-18 EP EP05808640.6A patent/EP1824601B1/en active Active
- 2005-10-18 US US11/252,821 patent/US20060093528A1/en not_active Abandoned
- 2005-10-18 US US11/252,914 patent/US8312890B1/en active Active
- 2005-10-18 EP EP05808694.3A patent/EP1828746B1/en active Active
- 2005-10-18 US US11/252,912 patent/US20060090800A1/en not_active Abandoned
- 2005-10-18 EP EP05809896.3A patent/EP1824600B1/en not_active Not-in-force
- 2005-10-18 WO PCT/US2005/037338 patent/WO2006044841A2/en active Application Filing
- 2005-10-18 WO PCT/US2005/037342 patent/WO2006044843A2/en active Application Filing
- 2005-10-18 US US11/252,915 patent/US8062611B2/en active Active
-
2009
- 2009-11-30 US US12/627,824 patent/US20100133104A1/en not_active Abandoned
- 2009-11-30 US US12/627,799 patent/US20100136701A1/en not_active Abandoned
-
2010
- 2010-11-12 US US12/945,793 patent/US8383062B2/en active Active
- 2010-11-24 US US12/954,519 patent/US8147770B2/en active Active
-
2012
- 2012-02-23 US US13/403,355 patent/US20120214156A1/en not_active Abandoned
Patent Citations (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4549952A (en) * | 1982-11-22 | 1985-10-29 | Eastman Kodak Company | Capillary transport device having means for increasing the viscosity of the transported liquid |
US4522923A (en) * | 1983-10-03 | 1985-06-11 | Genetic Diagnostics Corporation | Self-contained assay method and kit |
US4800066A (en) * | 1986-07-21 | 1989-01-24 | The Drackett Company | End of life indicator for automatic toilet cleaning devices |
US6152181A (en) * | 1992-11-16 | 2000-11-28 | The United States Of America As Represented By The Secretary Of The Air Force | Microdevices based on surface tension and wettability that function as sensors, actuators, and other devices |
US5798215A (en) * | 1993-02-18 | 1998-08-25 | Biocircuits Corporation | Device for use in analyte detection assays |
US5846396A (en) * | 1994-11-10 | 1998-12-08 | Sarnoff Corporation | Liquid distribution system |
US6102897A (en) * | 1996-11-19 | 2000-08-15 | Lang; Volker | Microvalve |
US6030581A (en) * | 1997-02-28 | 2000-02-29 | Burstein Laboratories | Laboratory in a disk |
US6302134B1 (en) * | 1997-05-23 | 2001-10-16 | Tecan Boston | Device and method for using centripetal acceleration to device fluid movement on a microfluidics system |
US20040089616A1 (en) * | 1997-05-23 | 2004-05-13 | Gregory Kellogg | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
US6375901B1 (en) * | 1998-06-29 | 2002-04-23 | Agilent Technologies, Inc. | Chemico-mechanical microvalve and devices comprising the same |
US20020054835A1 (en) * | 1998-06-29 | 2002-05-09 | Robotti Karla M. | Chemico-mechanical microvalve and devices comprising the same |
US20020153251A1 (en) * | 1999-02-03 | 2002-10-24 | Alexander Sassi | Multichannel control in microfluidics |
US20040175296A1 (en) * | 1999-11-15 | 2004-09-09 | Opalsky Cindra A. Widrig | Apparatus and method for assaying coagulation in fluid samples |
US6755621B2 (en) * | 2000-02-25 | 2004-06-29 | Science & Technology Corporation @ University Of New Mexico | Stimuli-responsive hybrid materials containing molecular actuators and their applications |
US20030210997A1 (en) * | 2000-02-25 | 2003-11-13 | Lopez Gabriel P. | Stimuli-responsive hybrid materials containing molecular actuators and their applications |
US6615855B2 (en) * | 2000-02-25 | 2003-09-09 | Science & Technology Corporation @T Unm | Stimuli-responsive hybrid materials containing molecular actuators and their applications |
US20020194909A1 (en) * | 2000-10-24 | 2002-12-26 | Hasselbrink Ernest F. | Mobile monolithic polymer elements for flow control in microfluidic devices |
US20040067168A1 (en) * | 2000-12-08 | 2004-04-08 | Frederic Buffiere | Temporary separation barrier, container comprising same and method for carrying out a test in said container |
US20020121487A1 (en) * | 2001-01-03 | 2002-09-05 | Robotti Karla M. | Methods and using chemico-mechanical microvalve devices for the selective separation of components from multi-component fluid samples |
US20020143437A1 (en) * | 2001-03-28 | 2002-10-03 | Kalyan Handique | Methods and systems for control of microfluidic devices |
US20030019522A1 (en) * | 2001-07-26 | 2003-01-30 | Gene Parunak | Methods and systems for fluid control in microfluidic devices |
US20040043507A1 (en) * | 2002-08-27 | 2004-03-04 | Kimberly-Clark Worldwide, Inc. | Fluidics-based assay devices |
US20040096358A1 (en) * | 2002-11-14 | 2004-05-20 | Gert Blankenstein | Device for the stepwise transport of liquid utilizing capillary forces |
US20040208792A1 (en) * | 2002-12-20 | 2004-10-21 | John Linton | Assay apparatus and method using microfluidic arrays |
US20050244504A1 (en) * | 2003-09-23 | 2005-11-03 | Little Steven R | pH triggerable polymeric particles |
US20060090800A1 (en) * | 2004-10-18 | 2006-05-04 | Applera Corporation | Fluid processing device including size-changing barrier |
US20100136701A1 (en) * | 2004-10-18 | 2010-06-03 | Life Technologies Corporation | Device including a dissolvable structure for flow control |
US20100133104A1 (en) * | 2004-10-18 | 2010-06-03 | Life Technologies Corporation | Fluid processing device including size-changing barrier |
US20110114206A1 (en) * | 2004-10-18 | 2011-05-19 | Life Technologies Corporation | Fluid Processing Device Including Size-Changing Barrier |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060093526A1 (en) * | 2004-10-18 | 2006-05-04 | Applera Corporation | Fluid processing device including composite material flow modulator |
US20110114206A1 (en) * | 2004-10-18 | 2011-05-19 | Life Technologies Corporation | Fluid Processing Device Including Size-Changing Barrier |
US8062611B2 (en) | 2004-10-18 | 2011-11-22 | Applied Biosystems, Llc | Fluid processing device including composite material flow modulator |
US8383062B2 (en) | 2004-10-18 | 2013-02-26 | Applied Biosystems, Llc | Fluid processing device including size-changing barrier |
WO2013153181A1 (en) * | 2012-04-13 | 2013-10-17 | Technische Universität Dresden | Method and device for targeted process control in a microfluidic processor having integrated active elements |
US9272281B2 (en) | 2012-04-13 | 2016-03-01 | Technische Universität Dresden | Method and device for targeted process control in a microfluidic processor having integrated active elements |
US10258984B2 (en) | 2014-06-16 | 2019-04-16 | Koninklijke Philips N.V. | Cartridge for fast sample intake |
Also Published As
Publication number | Publication date |
---|---|
EP1828746A2 (en) | 2007-09-05 |
EP1824600B1 (en) | 2016-12-28 |
US20110114206A1 (en) | 2011-05-19 |
US20100133104A1 (en) | 2010-06-03 |
EP1828746B1 (en) | 2016-12-28 |
US20100136701A1 (en) | 2010-06-03 |
EP1824601B1 (en) | 2016-12-28 |
US20060090800A1 (en) | 2006-05-04 |
WO2006044896A2 (en) | 2006-04-27 |
US8062611B2 (en) | 2011-11-22 |
US8147770B2 (en) | 2012-04-03 |
WO2006044843A3 (en) | 2006-12-14 |
WO2006044896A3 (en) | 2006-06-22 |
US20060093528A1 (en) | 2006-05-04 |
EP1824600A4 (en) | 2009-09-23 |
US8383062B2 (en) | 2013-02-26 |
US8312890B1 (en) | 2012-11-20 |
US20120214156A1 (en) | 2012-08-23 |
EP1824601A2 (en) | 2007-08-29 |
US20060093526A1 (en) | 2006-05-04 |
EP1828746A4 (en) | 2009-09-02 |
WO2006044841A2 (en) | 2006-04-27 |
EP1824601A4 (en) | 2009-09-02 |
WO2006044843A2 (en) | 2006-04-27 |
WO2006044841A3 (en) | 2009-04-16 |
EP1824600A2 (en) | 2007-08-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8147770B2 (en) | Microfluidic device including a dissolvable structure for flow control | |
US8628730B2 (en) | Sample substrate having a divided sample chamber and method of loading thereof | |
US6951632B2 (en) | Microfluidic devices for introducing and dispensing fluids from microfluidic systems | |
AU2006287825B2 (en) | Cartridge having variable volume reservoirs | |
US20060002827A1 (en) | Liquid reservoir connector | |
EP1946830A1 (en) | Microreactor and method of liquid feeding making use of the same | |
EP1679501A2 (en) | Chemical analysis apparatus and chemical analysis cartridge | |
US20110027904A1 (en) | Sample mixing on a microfluidic device | |
WO2008076395A2 (en) | Mechanically actuated diagnostic device | |
JP2005502062A (en) | Sample container | |
WO2018183744A1 (en) | Microfluidic device and methods | |
JP2003517368A (en) | Pumping device for moving at least one fluid into the consumable | |
MXPA01000691A (en) | Fluidic extraction of microdissected samples. | |
Xie et al. | Optimization of a microfluidic cartridge for Lab-on-a-chip (LOC) application and bio-testing for DNA/RNA extraction | |
US11701659B2 (en) | Microfluidic chip and droplet separation method | |
Pramanik | Development of highly functional microfluidic devices for biochemical analyses | |
KR20080007324A (en) | Methods and device for transmitting,enclosing and analysing fluid samples | |
Gärtner et al. | SmartHEALTH: a microfluidic multisensor platform for POC cancer diagnostics |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STCF | Information on status: patent grant |
Free format text: PATENTED CASE |
|
FPAY | Fee payment |
Year of fee payment: 4 |
|
MAFP | Maintenance fee payment |
Free format text: PAYMENT OF MAINTENANCE FEE, 8TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: M1552); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY Year of fee payment: 8 |
|
FEPP | Fee payment procedure |
Free format text: MAINTENANCE FEE REMINDER MAILED (ORIGINAL EVENT CODE: REM.); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |