US3577446A - Phosphatidylalkanolamine derivatives - Google Patents
Phosphatidylalkanolamine derivatives Download PDFInfo
- Publication number
- US3577446A US3577446A US758587A US3577446DA US3577446A US 3577446 A US3577446 A US 3577446A US 758587 A US758587 A US 758587A US 3577446D A US3577446D A US 3577446DA US 3577446 A US3577446 A US 3577446A
- Authority
- US
- United States
- Prior art keywords
- octadeca
- trienoyl
- tetraenoyl
- eicosa
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- -1 2-HYDROXYETHYL- Chemical group 0.000 abstract description 24
- 150000001875 compounds Chemical class 0.000 abstract description 24
- 238000000034 method Methods 0.000 abstract description 19
- 238000002360 preparation method Methods 0.000 abstract description 6
- NAXUFNXWXFZVSI-UHFFFAOYSA-N 4-aminobutan-2-ol Chemical compound CC(O)CCN NAXUFNXWXFZVSI-UHFFFAOYSA-N 0.000 abstract description 3
- 230000003276 anti-hypertensive effect Effects 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 27
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 26
- 239000000243 solution Substances 0.000 description 23
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 20
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 18
- 125000001527 trienoyl group Chemical group 0.000 description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 13
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 239000002253 acid Substances 0.000 description 10
- 239000011541 reaction mixture Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 9
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 9
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 9
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 125000002252 acyl group Chemical group 0.000 description 7
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 7
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 7
- 159000000009 barium salts Chemical class 0.000 description 7
- QWOJMRHUQHTCJG-UHFFFAOYSA-N CC([CH2-])=O Chemical compound CC([CH2-])=O QWOJMRHUQHTCJG-UHFFFAOYSA-N 0.000 description 6
- 238000010828 elution Methods 0.000 description 6
- 150000002148 esters Chemical class 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 6
- SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 5
- 150000005690 diesters Chemical class 0.000 description 5
- 125000005639 glycero group Chemical group 0.000 description 5
- 150000007530 organic bases Chemical class 0.000 description 5
- 235000011007 phosphoric acid Nutrition 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 5
- 235000012239 silicon dioxide Nutrition 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical compound CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 4
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 4
- RNVYQYLELCKWAN-YFKPBYRVSA-N [(4s)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol Chemical compound CC1(C)OC[C@H](CO)O1 RNVYQYLELCKWAN-YFKPBYRVSA-N 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000003208 petroleum Substances 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
- 229910052938 sodium sulfate Inorganic materials 0.000 description 4
- 235000011152 sodium sulphate Nutrition 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- FQFILJKFZCVHNH-UHFFFAOYSA-N tert-butyl n-[3-[(5-bromo-2-chloropyrimidin-4-yl)amino]propyl]carbamate Chemical compound CC(C)(C)OC(=O)NCCCNC1=NC(Cl)=NC=C1Br FQFILJKFZCVHNH-UHFFFAOYSA-N 0.000 description 4
- 150000005691 triesters Chemical class 0.000 description 4
- XVMSFILGAMDHEY-UHFFFAOYSA-N 6-(4-aminophenyl)sulfonylpyridin-3-amine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=N1 XVMSFILGAMDHEY-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical group CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WUGQZFFCHPXWKQ-UHFFFAOYSA-N Propanolamine Chemical compound NCCCO WUGQZFFCHPXWKQ-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229960000583 acetic acid Drugs 0.000 description 3
- 125000006242 amine protecting group Chemical group 0.000 description 3
- ROLQPIOIXBZPFD-UHFFFAOYSA-N bis(2,2,2-trichloroethyl) hydrogen phosphate Chemical compound ClC(Cl)(Cl)COP(=O)(O)OCC(Cl)(Cl)Cl ROLQPIOIXBZPFD-UHFFFAOYSA-N 0.000 description 3
- 150000004650 carbonic acid diesters Chemical class 0.000 description 3
- 150000001805 chlorine compounds Chemical class 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- MAAVGFJWXMUHAT-UHFFFAOYSA-N octadeca-6,9,12-trienoyl chloride Chemical compound CCCCCC=CCC=CCC=CCCCCC(Cl)=O MAAVGFJWXMUHAT-UHFFFAOYSA-N 0.000 description 3
- 125000001095 phosphatidyl group Chemical group 0.000 description 3
- XKJCHHZQLQNZHY-UHFFFAOYSA-N phthalimide Chemical compound C1=CC=C2C(=O)NC(=O)C2=C1 XKJCHHZQLQNZHY-UHFFFAOYSA-N 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- GPTXCAZYUMDUMN-UHFFFAOYSA-N tert-butyl n-(2-hydroxyethyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCO GPTXCAZYUMDUMN-UHFFFAOYSA-N 0.000 description 3
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 3
- NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 description 2
- LJCZNYWLQZZIOS-UHFFFAOYSA-N 2,2,2-trichlorethoxycarbonyl chloride Chemical compound ClC(=O)OCC(Cl)(Cl)Cl LJCZNYWLQZZIOS-UHFFFAOYSA-N 0.000 description 2
- CRAJLXHBNCCDPK-UHFFFAOYSA-N 2,2,2-trichloroethyl hydrogen carbonate Chemical compound OC(=O)OCC(Cl)(Cl)Cl CRAJLXHBNCCDPK-UHFFFAOYSA-N 0.000 description 2
- AOOVAHXXKSHJSM-UHFFFAOYSA-N 2-(3-hydroxybutyl)isoindole-1,3-dione Chemical class C1=CC=C2C(=O)N(CCC(O)C)C(=O)C2=C1 AOOVAHXXKSHJSM-UHFFFAOYSA-N 0.000 description 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- BLFRQYKZFKYQLO-UHFFFAOYSA-N 4-aminobutan-1-ol Chemical compound NCCCCO BLFRQYKZFKYQLO-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Chemical compound OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- DRUIESSIVFYOMK-UHFFFAOYSA-N Trichloroacetonitrile Chemical compound ClC(Cl)(Cl)C#N DRUIESSIVFYOMK-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 229940030600 antihypertensive agent Drugs 0.000 description 2
- 239000002220 antihypertensive agent Substances 0.000 description 2
- AYJRCSIUFZENHW-UHFFFAOYSA-L barium carbonate Chemical compound [Ba+2].[O-]C([O-])=O AYJRCSIUFZENHW-UHFFFAOYSA-L 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 239000012259 ether extract Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 2
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 2
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 2
- 125000000654 isopropylidene group Chemical group C(C)(C)=* 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 150000008104 phosphatidylethanolamines Chemical class 0.000 description 2
- LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 2
- 125000005543 phthalimide group Chemical group 0.000 description 2
- 125000005544 phthalimido group Chemical group 0.000 description 2
- 125000001557 phthalyl group Chemical group C(=O)(O)C1=C(C(=O)*)C=CC=C1 0.000 description 2
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 2
- AOJFQRQNPXYVLM-UHFFFAOYSA-N pyridin-1-ium;chloride Chemical compound [Cl-].C1=CC=[NH+]C=C1 AOJFQRQNPXYVLM-UHFFFAOYSA-N 0.000 description 2
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- JIWBIWFOSCKQMA-GFRMADBLSA-N (6e,9e,12e,15e)-octadeca-6,9,12,15-tetraenoic acid Chemical compound CC\C=C\C\C=C\C\C=C\C\C=C\CCCCC(O)=O JIWBIWFOSCKQMA-GFRMADBLSA-N 0.000 description 1
- ZHHCWQGVXYGWCW-UHFFFAOYSA-N 1,1,1-trichloro-2-[chloro(2,2,2-trichloroethoxy)phosphoryl]oxyethane Chemical compound ClC(Cl)(Cl)COP(Cl)(=O)OCC(Cl)(Cl)Cl ZHHCWQGVXYGWCW-UHFFFAOYSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 125000000453 2,2,2-trichloroethyl group Chemical group [H]C([H])(*)C(Cl)(Cl)Cl 0.000 description 1
- DTOSIQBPPRVQHS-IUQGRGSQSA-N Elaidolinolenic acid Chemical compound CC\C=C\C\C=C\C\C=C\CCCCCCCC(O)=O DTOSIQBPPRVQHS-IUQGRGSQSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ASGJEMPQQVNTGO-UHFFFAOYSA-N benzene chloroform Chemical compound C(Cl)(Cl)Cl.C1=CC=CC=C1.C1=CC=CC=C1 ASGJEMPQQVNTGO-UHFFFAOYSA-N 0.000 description 1
- WPUJEWVVTKLMQI-UHFFFAOYSA-N benzene;ethoxyethane Chemical compound CCOCC.C1=CC=CC=C1 WPUJEWVVTKLMQI-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000002026 chloroform extract Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 150000002169 ethanolamines Chemical class 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001631 hypertensive effect Effects 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- MRKXCQPDRPTZCG-UHFFFAOYSA-N octadeca-9,12,15-trienoyl chloride Chemical compound CCC=CCC=CCC=CCCCCCCCC(Cl)=O MRKXCQPDRPTZCG-UHFFFAOYSA-N 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000008103 phosphatidic acids Chemical class 0.000 description 1
- 229940067605 phosphatidylethanolamines Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- RNVYQYLELCKWAN-UHFFFAOYSA-N solketal Chemical compound CC1(C)OCC(CO)O1 RNVYQYLELCKWAN-UHFFFAOYSA-N 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- XDJCYKMWJCYQJM-UHFFFAOYSA-N tert-butyl n-(3-hydroxypropyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCCO XDJCYKMWJCYQJM-UHFFFAOYSA-N 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
- C07F9/65515—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a five-membered ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
Definitions
- This invention relates to new phosphatidylalkanolamine derivatives and to processes used for their synthesis.
- this invention relates to phosphatidylalkanolamine derivatives of Formula I,
- R and R represent the same or different acyl group containing 18 to 20 carbon atoms and three or more double bonds, such as, for example, an octadeca- 6,9,12-trienoyl, octadeca-9,12,15-trienoyl, octadeca-6,9, 12,15-tetraenoyl, eic0sa-8,11,14-trienoyl or an eicosa-5,8, 11,14-tetraenoyl group; n represents the integers one or two; and R represents a hydrogen atom or a lower alkyl group, such as, for example, a methyl group.
- the phosphatidylalkanolamine derivatives of this invention have been found to possess pharmacological properties which render them useful as medicinal agents. More particularly, these derivatives exhibit utility as antihypertensive agents when tested in standard pharmacological tests. For example, when these derivatives are administered to renal hypertensive rats, obtained by the method of A. Grollman, Proc. Soc. Exptl. Biol. Med., 57, 102 (1944), reduction of blood pressure toward normal levels is observed. This fall in blood pressure is readily measured by the method of H. Kersten et al., J. Lab. Clin. Med., 32, 1090 (1947).
- the compounds of this invention are employed as antihypertensive agents in warm-blooded animals, e.g. rats, alone or in combination with pharmacologically acceptable carriers, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration and standard biological practice.
- they may be administered orally in solid form containing such excipients as starch, milk sugar, certain types of clay and so forth.
- They may also be administered orally in the form of solutions or they may be injected parenterally.
- parenteral administration may be used in the form of a sterile solution containing other solutes, for example, enough saline or glucose to make the solution isotonic.
- the dosage of the present therapeutic agents will vary with the form of administration and the particular compound chosen. Furthermore, it will vary with the particular host under treatment. Generally, treatment is initiated with small dosages substantially less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached.
- the compounds of this invention are most desirably administered at a concentration level that will generally afford efiective results without causing any harmful or deleterious side eifects and preferably at a level that is in a range of from about 0.3 mg. to about mg. per kilo per day, although as aforementioned variations will occur. However, a dosage level that is in the range of from about 3 mg. to about 10 mg. per kilo per day is most satisfactory. Such doses may be administered once or twice a day, as required.
- a noteworthy aspect of this invention is the discovery that a minimum of three double bonds must be present in the acyl groups of the phosphatidylalkanolarnines of Formula I before said compounds exhibit antihypertensive activity.
- 1,2-di-(octadeca-9,12-dienoyl)-snglycero 3 phosphorylethanolamine (containing two double bonds per acyl group) (I; R and R -CH (CH 3 (CH CH CH) 2 (CH COO CHzO HBO CH3 CHO on O H R III.
- R R R and n are as defined above and Z represents a primary amino group coupled with an amineprotecting group, such as, preferably a phthalyl group or some other amine-protecting group, for example, such as those described E. Schriider and K. Liibke in The Peptides, vol. I, Academic Press, New York, 1965, pp. 3-51.
- an amineprotecting group such as, preferably a phthalyl group or some other amine-protecting group, for example, such as those described E. Schriider and K. Liibke in The Peptides, vol. I, Academic Press, New York, 1965, pp. 3-51.
- the starting material of Formula H may be 1,2-isopropylidene-sn-glycerol, described by E. Baer, Biochem. Prep, 2, 31 (1952) or rac-1,2-isopropylidineglycero1, de-
- the starting material of Formula II is condensed with phosphorus oxychloride in the presence of an organic base, preferably quinoline, followed by treatment with the appropriate 2- or 3-hydroxyalkylphthalimide described below. In this manner the glycerophosphoric acid diester acetonide of Formula III is obtained.
- the 2- and 3-hydroxyalkylphthalimides preferred in the above reaction are N-(2-hydroxyethyl)-, N-(3-hydroxypropyl)-, N-(2-hydroxypropyl)- and N-(3-hydroxybutyl) phthalimides.
- the first two compounds are described by F. Garelli and G. Racciu, Atti accad. sci. Torino, Classe sci. fis., mat. nat., 69, 358 (1934); Chem. Abstn, 29, 6223 (1935); the latter two compounds are described by S. Gabriel and H. Ohle, Chem. Ber., 50, 819 (1917) and R. Robinson and H. Suginome, J. Chem. Soc., 304 (1932), respectively.
- the glycerophosphoric acid diester acetonide of Formula III is subjected to mild hydrolyzing conditions, preferably by bringing said diester of Formula III into contact with an acidic ion exchange resin in the presence of an aqueous medium, such as 80% aqueous methanol. Such conditions remove the acetone group and yield the corresponding glycerophosphoric acid diester of Formula Alternatively, hydrolysis with a dilute acid, such as, for example dilute acetic acid, may be used to achieve the removal of the acetone group yielding the diester of Formula IV.
- a dilute acid such as, for example dilute acetic acid
- esterification of the free hydroxy groups of the glycerophosphoric acid diester IV said ester is converted to a corresponding, heavy metal salt, preferably the barium salt.
- a corresponding, heavy metal salt preferably the barium salt.
- the preparation of this salt serves two purposes. First, a greater stability is imparted to said ester, facilitating its purification and isolation. Secondly, participation of the acidic hydroxy group of the phosphoric acid portion of the molecule in the esterification step is blocked.
- the conversion of the glycerophosphoric acid diester IV to the corresponding diacyl derivative of Formula V is readily achieved by acylation of a heavy metal salt, for example, the barium salt, of the glycerophosphoric acid diester of Formula IV by conventional methods, such as, for example, the use of an acylating agent, such as, an appropriate acid chloride, in the presence of pyridine.
- a heavy metal salt for example, the barium salt
- an acylating agent such as, an appropriate acid chloride
- the preferred acylating agents for the above reactions are the corresponding acid chlorides, prepared from the appropriate acids, according to the method used by W. Stofi'el and H. D. Pruss, J. Lipid Res, 8, 196 (1967) for the preparation of octadeca-6,9,12-trienoyl chloride.
- Such acids are octadeca-6,9,l2-trienoic acid, described by J. M. Osbond and J. C. Wickers, Chem. and 1nd, 1287 (1959); octadeca-9,12,15-trienoic acid, described by S. S. Nigam and B. C. L. Weedon, Chem.
- the protective group for example, the phthalyl group of the diacyl derivatives of Formula V is removed by conventional treatment with hydrazine hydrate (see for example, E. Schrtider and H. Liibke, cited above) to yield the desired phosphatidylalkanolamine derivatives of this invention.
- the compounds of this invention may be synthesized by a process illustrated by the following formulae:
- R R R n and Z are as defined above.
- the starting material of Formula II is condensed in the presence of an organic base, preferably pyridine, with di-(2,2,2-trichloroethy1)phosphorochloridate (VI) described by F. Eckstein and K. H. Scheit, Angew. Chem. Internat. edit., 6, 362 (1967), to yield the phosphoric acid triester acetonide of Formula VII.
- the triester acetonide VII is converted to the triester of Formula VIII by mild hydrolyzing methods, such as described above for the conversion of the compounds of Formula III to the compounds of Formula IV.
- the triester VIII is then treated with appropriate acylating agents, described above, to yield the diacyl phosphoric acid triester of Formula IX.
- R R R and n are as defined above and Z represents a primary amino group coupled with a tertbutyloxycarbonyl amine-protecting group.
- the starting material of Formula II is condensed in the presence of an organic base, preferably pyridine with (2,2,2-trichloroethoxy)carbonyl chloride of Formula XI, described by T. B. Windholz and D. B. R. Johnston, Tetrahedron Letters, 2555 (1967), to yield the carbonic acid diester acetonide of Formula XII.
- the acetone group of the acetonide XII is removed to yield the carbonic acid diester of Formula XIII by mild hydrolyzing methods, such as described above for the conversion of the compounds of Formula III to the compounds of Formula IV.
- the diester XIII is then treated with appropriate acylating agents, described above, to yield the diacyl carbonic acid diester of Formula XIV.
- appropriate acylating agents described above
- the latter compound upon treatment with zinc in acetic acid, readily yields the glycerol diacylate of Formula XV which may be condensed with phosphorus oxychloride in the presence of an Organic base, preferably quinoline, followed by treatment with an appropriate hydroxyalkylcarbarnic acid tert-butyl ester, described below, to afford the compounds of.
- Formula V in which R and R are as defined above and Z represents a primary amino group coupled with a tertbutyloxycarbonyl group.
- hydroxyalkylcarbamic acid tert-butyl esters preferred in the above reaction are 2-hydroxyethylcarbamic acid tert-butyl ester, described by F. I. M. Daemen et al., Rec. Trav. Chim., 82, 487 (1963), 3-hydroxypropylcarbamic acid tertbutyl ester and 3-hydroxyb'utylcarbamic acid tertbutyl ester.
- the latter three esters may be readily prepared according to the procedure of F. J. M. Daemen et al., cited above, used for the preparation of the former ester. When employing this procedure for this purpose, an equivalent amount of S-amino-l-propanol, described by L.
- the manipulative procedure described in this example may be employed using an appropriate acid chloride, described above, and glycerophosphoryl- N-hydroxyalkylphthalimide, prepared as described in Example l, in a molar ratio of 0.8:1, followed by a repetition of said manipulative procedure using a three molar excess of a different acid chloride, described above, relative to the amount of said glycerophosphoryl-N-hydroxyalkylphthalimide, to yield the mixed diacylated phosphatidyl-N-hydroxyalkylphthalimides, 1-(octadeca-6,9,12-trienoyl) 2 (octadeca-9,12,15-trienoyl)-, -2-(octadeca-6,9, 12,l-tetraenoyl)-, -2-(eicosa-8,11,14-trien0yl)-, -2-(eicosa-5,8,l1,14-tetraeno
- the corresponding sn phosphatidylethanolamine derivatives 1,2 di (octadeca 6,9,12 trienoyl), 1,2 di (octadeca 9,12,15 trienoyl), 1,2 di (octadeca 6,9,12,15- tetraenoyl), 1,2 di (eicosa 8,11,14 trienoyl), 1,2- di (eicosa 5,8,11,14 tetraenoyl) esters of sn glycero- 3 phosphoryl (3 hydroxypropylamine), -(2 hydroxypropylamine), and -(3 hydroxybutylamine), are obtained.
- EXAMPLE 6 By using the manipulative procedures and the appropriate acid chlorides, described in Example 2, but substituting an equivalent amount of snor rac-glycero- 3-phosphroic acid di-(2,2,2-trichloroethyl)-ester, prepared as described in Example 5 in place of the glycerophosphoryl N hydroxylalkylphthalimide, followed by treatment with an excess of zinc dust in 80% aqueous acetic acid for one hour at room temperature to remove the 2,2,2-trichloroethyl ester groups the corresponding snor racphosphatidic acid derivatives, 1,2 di (octadeca 6,9,12- trienoyl)-, 1,2 di (octadeca 9,12,15 trienoyl)-, 1,2- di (octadeca 6,9,12,15 tetraenoyl)-, 1,2 di (eicosa- 8,11,14 trienoyl)-, 1,2 di (eicosa
- EXAMPLE 7 A solution of 7.0 g. of the phosphatidic acid derivative 1,2 di-(octadeca 9,12,15 trienoyl)-sn-glycero-3-phosphoric acid, prepared as described in Example 5, 1.8 g. of the hydroxyalkylphthalimide, N-(2 hydroxyethyl)phtha1- imide, and 5.00 g. of trichloroacetonitrile, described by W. Steinkopf, Chem.
- EXAMPLE 8 A solution of 1.0 g. of (2,2,2-trichloroethoxy)carbonyl chloride (X1) in 5 ml. of dry ether is added slowly to a vigorously stirred ice-cold solution of 500 mg. of 1,2-isopropylidine-sn-glycerol (II) and 1 ml. of pyridine in 5 ml. of dry ether. After stirring at room temperature for one hour, the reaction mixture is diluted with ether. The resultant precipitate of pyridine hydrochloride is removed by filtration. The filtrate is washed successively with cold 2 N-hydrochloric acid and water, dried over sodium sulfate, filtered and evaporated to dryness. The oily product, 1,2 isopropylidine-sn-glycero 3 carbonic acid (2,2,2- trichloroethyl) -ester (XII),
- EXAMPLE 11 To a solution of 0.46 g. of freshly distilled phosphorous oxychloride in ml. of dry methylene chloride at 0 0,, is added during 30 min. a solution of 1,2 di-(octadeca- 9,12,15 trienoyl)-sn-glycerol, prepared as described in Example 10, and 0.43 g. of dry quinoline in 10 ml. of dry methylene dichloride. The reaction mixture is kept at room temperature for one hour. The temperature of the reaction mixture is again reduced to 0 C. and a solution of 0.48 g. of 2-hydroxyethylcarbamic acid tert-butyl ester and 0.8 g. of pyridine in 10 ml.
Abstract
THERE ARE DISCLOSED HEREIN PHOSPHATIDYLALKANOLAMINES IN WHICH THE ALKANOLAMINE IS 2-HYDROXYETHYL-, 2- OR 3-HYDROXYPROPYL-, OR 3-HYDROXYBUTYLAMINE AND THE ACYL GROUPS CONTAIN FROM 18-20 CARBON ATOMS AND THREE OR MORE DOUBLE BONDS. THE COMPOUNDS HAVE ANTI-HYPERTENSIVE PROPERTIES, AND METHODS FOR THEIR PREPARATION AND USE ARE ALSO DISCLOSED.
Description
United States Patent 3,577,446 PHOSPHATIDYLALKANOLAMINE DERIVATIVES Sumanas Rakhit, Dollard des Ormeaux, Quebec, Canada, assignor to American Home Products Corporation, New York, N.Y. No Drawing. Filed Sept. 9, 1968, Ser. No. 758,587 Int. Cl. A23j 7/00 US. Cl. 260-403 1 Claim ABSTRACT OF THE DISCLOSURE There are disclosed herein phosphatidylalkanolamines in which the alkanolamine is 2-hydroxyethyl-, 2- or 3-hydroxypropyl-, or 3-hydroxybutylamine and the acyl groups contain from 18-20 carbon atoms and three or more double bonds. The compounds have anti-hypertensive properties, and methods for their preparation and use are also disclosed.
This invention relates to new phosphatidylalkanolamine derivatives and to processes used for their synthesis.
More specifically, this invention relates to phosphatidylalkanolamine derivatives of Formula I,
in which R and R represent the same or different acyl group containing 18 to 20 carbon atoms and three or more double bonds, such as, for example, an octadeca- 6,9,12-trienoyl, octadeca-9,12,15-trienoyl, octadeca-6,9, 12,15-tetraenoyl, eic0sa-8,11,14-trienoyl or an eicosa-5,8, 11,14-tetraenoyl group; n represents the integers one or two; and R represents a hydrogen atom or a lower alkyl group, such as, for example, a methyl group.
The phosphatidylalkanolamine derivatives of this invention have been found to possess pharmacological properties which render them useful as medicinal agents. More particularly, these derivatives exhibit utility as antihypertensive agents when tested in standard pharmacological tests. For example, when these derivatives are administered to renal hypertensive rats, obtained by the method of A. Grollman, Proc. Soc. Exptl. Biol. Med., 57, 102 (1944), reduction of blood pressure toward normal levels is observed. This fall in blood pressure is readily measured by the method of H. Kersten et al., J. Lab. Clin. Med., 32, 1090 (1947).
When the compounds of this invention are employed as antihypertensive agents in warm-blooded animals, e.g. rats, alone or in combination with pharmacologically acceptable carriers, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration and standard biological practice. For example, they may be administered orally in solid form containing such excipients as starch, milk sugar, certain types of clay and so forth. They may also be administered orally in the form of solutions or they may be injected parenterally. For parenteral administration they may be used in the form of a sterile solution containing other solutes, for example, enough saline or glucose to make the solution isotonic.
The dosage of the present therapeutic agents will vary with the form of administration and the particular compound chosen. Furthermore, it will vary with the particular host under treatment. Generally, treatment is initiated with small dosages substantially less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached. In general, the compounds of this invention are most desirably administered at a concentration level that will generally afford efiective results without causing any harmful or deleterious side eifects and preferably at a level that is in a range of from about 0.3 mg. to about mg. per kilo per day, although as aforementioned variations will occur. However, a dosage level that is in the range of from about 3 mg. to about 10 mg. per kilo per day is most satisfactory. Such doses may be administered once or twice a day, as required.
A noteworthy aspect of this invention is the discovery that a minimum of three double bonds must be present in the acyl groups of the phosphatidylalkanolarnines of Formula I before said compounds exhibit antihypertensive activity. For example, 1,2-di-(octadeca-9,12-dienoyl)-snglycero 3 phosphorylethanolamine (containing two double bonds per acyl group) (I; R and R -CH (CH 3 (CH CH CH) 2 (CH COO CHzO HBO CH3 CHO on O H R III.
OH R.
in which R R R and n are as defined above and Z represents a primary amino group coupled with an amineprotecting group, such as, preferably a phthalyl group or some other amine-protecting group, for example, such as those described E. Schriider and K. Liibke in The Peptides, vol. I, Academic Press, New York, 1965, pp. 3-51.
The starting material of Formula H may be 1,2-isopropylidene-sn-glycerol, described by E. Baer, Biochem. Prep, 2, 31 (1952) or rac-1,2-isopropylidineglycero1, de-
3 scribed by M. S. Newman and M. Renoll, J. Am. Chem. Soc., 67, 1621 (1945).
The starting material of Formula II is condensed with phosphorus oxychloride in the presence of an organic base, preferably quinoline, followed by treatment with the appropriate 2- or 3-hydroxyalkylphthalimide described below. In this manner the glycerophosphoric acid diester acetonide of Formula III is obtained.
The 2- and 3-hydroxyalkylphthalimides preferred in the above reaction are N-(2-hydroxyethyl)-, N-(3-hydroxypropyl)-, N-(2-hydroxypropyl)- and N-(3-hydroxybutyl) phthalimides. The first two compounds are described by F. Garelli and G. Racciu, Atti accad. sci. Torino, Classe sci. fis., mat. nat., 69, 358 (1934); Chem. Abstn, 29, 6223 (1935); the latter two compounds are described by S. Gabriel and H. Ohle, Chem. Ber., 50, 819 (1917) and R. Robinson and H. Suginome, J. Chem. Soc., 304 (1932), respectively.
The glycerophosphoric acid diester acetonide of Formula III is subjected to mild hydrolyzing conditions, preferably by bringing said diester of Formula III into contact with an acidic ion exchange resin in the presence of an aqueous medium, such as 80% aqueous methanol. Such conditions remove the acetone group and yield the corresponding glycerophosphoric acid diester of Formula Alternatively, hydrolysis with a dilute acid, such as, for example dilute acetic acid, may be used to achieve the removal of the acetone group yielding the diester of Formula IV.
Before commencing with the next step, the esterification of the free hydroxy groups of the glycerophosphoric acid diester IV, said ester is converted to a corresponding, heavy metal salt, preferably the barium salt. The preparation of this salt serves two purposes. First, a greater stability is imparted to said ester, facilitating its purification and isolation. Secondly, participation of the acidic hydroxy group of the phosphoric acid portion of the molecule in the esterification step is blocked.
Accordingly, the conversion of the glycerophosphoric acid diester IV to the corresponding diacyl derivative of Formula V is readily achieved by acylation of a heavy metal salt, for example, the barium salt, of the glycerophosphoric acid diester of Formula IV by conventional methods, such as, for example, the use of an acylating agent, such as, an appropriate acid chloride, in the presence of pyridine. When employing the conventional method of using an acid chloride in the presence of pyridine it is an advantage to use dimethylformamide as a solvent for the reaction, since a homogeneous reaction medium, and consequently superior yields are obtained. When this step is used in the process directed to the preparation of the compounds of this invention of general Formula I in which R and R are the same acyl group, an amount more than two equivalents, preferably five equivalents, of the acylating agent are employed. On the other hand, for the process directed toward the compounds of general Formula I in which R and R are different acyl groups, between 0.5 and 1.2 equivalents, preferably 0.8-1.0 equivalent, of the appropriate acylating agent, is first allowed to react with the heavy metal salt of the glycerophosphoric acid diester of Formula IV according to the conventional acylating methods, described above, to yield the corresponding derivative possessing an acylated primary hydroxy group. Subsequent treatment of the latter derivative, also as the heavy metal salt, for example, the barium salt, with an excess, preferably three equivalents of a different acylating agent, chosen accordingly, and using the same acylating conditions, affords the diacyl derivative of Formula V in which R and R represent different acyl groups.
The preferred acylating agents for the above reactions are the corresponding acid chlorides, prepared from the appropriate acids, according to the method used by W. Stofi'el and H. D. Pruss, J. Lipid Res, 8, 196 (1967) for the preparation of octadeca-6,9,12-trienoyl chloride. Such acids are octadeca-6,9,l2-trienoic acid, described by J. M. Osbond and J. C. Wickers, Chem. and 1nd, 1287 (1959); octadeca-9,12,15-trienoic acid, described by S. S. Nigam and B. C. L. Weedon, Chem. and Ind., (1955); octadeca-6,9,12,15-tetraenoic acid, described by M. Matic, Biochem. J., 68, 692 (1958); eicosa- 8,ll,l4-trienoic acid, described by W. Stoffel, Ann. Chem, 673, 26 (1964); and eicosa-5,8,l1,14-tetraenoic acid, described by J. M. Osbond and J. C. Wickers, cited above.
Finally, the protective group for example, the phthalyl group of the diacyl derivatives of Formula V, is removed by conventional treatment with hydrazine hydrate (see for example, E. Schrtider and H. Liibke, cited above) to yield the desired phosphatidylalkanolamine derivatives of this invention.
Alternatively, the compounds of this invention may be synthesized by a process illustrated by the following formulae:
in which R R R n and Z are as defined above.
The starting material of Formula II, described above, is condensed in the presence of an organic base, preferably pyridine, with di-(2,2,2-trichloroethy1)phosphorochloridate (VI) described by F. Eckstein and K. H. Scheit, Angew. Chem. Internat. edit., 6, 362 (1967), to yield the phosphoric acid triester acetonide of Formula VII. The triester acetonide VII is converted to the triester of Formula VIII by mild hydrolyzing methods, such as described above for the conversion of the compounds of Formula III to the compounds of Formula IV. The triester VIII is then treated with appropriate acylating agents, described above, to yield the diacyl phosphoric acid triester of Formula IX. The latter compound on treatment with zinc in acetic acid affords the corresponding diacyl phosphoric acid monoester of Formula X, which may be condensed, for example, in the presence of an organic base, preferably pyridine, and trichloroacetonitrile, with the appropriate 2- or 3-hydroxy-alkylphthalimide, described above, to yield the penultimate intermediate in this process, the phosphatidyl derivative V. This phosphatidyl derviative is also the penultimate intermediate for the preceding process and its conversion to the compounds of this invention of formula is described above.
Again alternatively, the compounds of this invention may be synthesized by a process illustrated by the following formulae:
in which R R R and n are as defined above and Z represents a primary amino group coupled with a tertbutyloxycarbonyl amine-protecting group.
The starting material of Formula II, described above, is condensed in the presence of an organic base, preferably pyridine with (2,2,2-trichloroethoxy)carbonyl chloride of Formula XI, described by T. B. Windholz and D. B. R. Johnston, Tetrahedron Letters, 2555 (1967), to yield the carbonic acid diester acetonide of Formula XII. The acetone group of the acetonide XII is removed to yield the carbonic acid diester of Formula XIII by mild hydrolyzing methods, such as described above for the conversion of the compounds of Formula III to the compounds of Formula IV. The diester XIII is then treated with appropriate acylating agents, described above, to yield the diacyl carbonic acid diester of Formula XIV. The latter compound, upon treatment with zinc in acetic acid, readily yields the glycerol diacylate of Formula XV which may be condensed with phosphorus oxychloride in the presence of an Organic base, preferably quinoline, followed by treatment with an appropriate hydroxyalkylcarbarnic acid tert-butyl ester, described below, to afford the compounds of. Formula V in which R and R are as defined above and Z represents a primary amino group coupled with a tertbutyloxycarbonyl group.
The appropriate hydroxyalkylcarbamic acid tert-butyl esters preferred in the above reaction are 2-hydroxyethylcarbamic acid tert-butyl ester, described by F. I. M. Daemen et al., Rec. Trav. Chim., 82, 487 (1963), 3-hydroxypropylcarbamic acid tertbutyl ester and 3-hydroxyb'utylcarbamic acid tertbutyl ester. The latter three esters may be readily prepared according to the procedure of F. J. M. Daemen et al., cited above, used for the preparation of the former ester. When employing this procedure for this purpose, an equivalent amount of S-amino-l-propanol, described by L. Henry, Chem. Ber., 33, 3169 (1900), l-amino-Z-propanol, described by P. A. Levene and J. Scheidegger, J. Biol. Chem, '60, 172 (1924) and 4- amino-2-butanol, described by R. Robinson and H. Suginome, cited above, is used instead of ethanolamine to obtain the corresponding hydroxyalkylcarbamic acid tertbutyl ester, respectively.
The compounds of Formula V in which R and R are as defined above and Z represents a primary amino group coupled with a tert-butyloxycarbonyl group are readily converted to the phosphatidylalkanolamine derivatives of 6 Formula I by conventional methods such as those described by E. Schrtider and K. Liibke, cited above, see page 39; the use of hydrogen chloride in ether solution is a preferred conventional method.
The following examples will illustrate this invention.
EXAMPLE 1 To a stirred solution of 4.6 ml. of freshly distilled phosphorous oxychloride in 25 ml. of dry methylene chloride at 15 C., is added during min. a solution of 1,2-isopropylidene-sn-glycerol (II, 6.6 g.) and 7 ml. of distilled quinoline in ml. of dry methylene chloride. The reaction mixture is kept at 35 C. for 90 min. The temperature of the reaction mixture is again lowered to 10 C. and over a period of 60 min. a solution of 9.5 g. of N-(Z-hydroxyethyl)phthalimide and 16.1 ml. of dry pyridine in ml. dry methylene chloride is added. The reaction mixture is left at room temperature for 18 hours. Water (1.25 ml.) is added to the reaction mixture with continuous stirring and the solvent is removed under reduced pressure at a temperature not exceeding 40 C. The oily residue is triturated successively with three 125 ml. portions of petroleum ether and three 125 ml. of dry ether and then exhaustively extracted with six 125 ml. portions of benzene. The combined benzene extract is evaporated to dryness under reduced pressure at EEO-35 C. affording 1,2 isopropylidene sn glycero-3-phosphoryl-N-(2-hydroxyethyl) phthalimide (III; R =H, n=1 and Z=phthalimido),
1775 and 1740 cm.- as a glassy residue. This residue is dissolved in 250 ml. of distilled water and allowed to stand at room temperature for 4 hours, then freed from insoluble materials by extraction with ether. To the clear aqueous solution, 8.75 g. of barium carbonate is added slowly with continuous stirring. After one hour the mixture is filtered and the filtrate centrifuged to remove colloidal particles. The clear supernatant is evaporated under reduced pressure at 3540 C. to yield a glassy solid. This solid is readily soluble in Water, methanol and dimethylformamide and insoluble in ether, benzene and chloroform. It is characterized as the barium salt of sn-glycero- 3 phosphoryl N (2-hydroxyethyl)-phthalimide by its analysis: Calculated for C H N O P Ba (percent): N, 3.39 and P, 7.50. Found (percent): N, 3.40 and P, 7.67.
In the same manner, but using an equivalent amount of N-(3-hydroxypropyl)-, N-(2 hydroxypropyl)-, or N-(3-hydroxybutyl)phthalimide instead of N-(Z-hydroxyethyl)phthalimide, the glycerophosphoryl-N-hydroxyalkylphthalimides, sn glycero-3-phosphoryl-N-(3-hydroxypropyl)-, -N-(2-hydroxypropyl)-, and -N-(3-hydroxybutyl)- phthalimides and their corresponding barium salts are obtained, respectively.
In the same manner, but using an equivalent amount of rac-1,2-isopropylidineglycerol instead of 1,2-isopropylidene-sn-glycerol, and the appropriate N-(2- or N-(3-hydroxyalkyl)phthalimide, described above, the corresponding racemic mixtures of sn-glycero-3-phosphoryl-N-(2-hydroxyethyl)-, -N-(3-hydroxypropyl)-, -N-(2-hydroxypropyl)-, and -N-(3-hydroxybutyl)phthalimides, and their corresponding barium salts, are obtained.
EXAMPLE 2 To a solution of the thoroughly dried barium salt of the glycerophosphoryl-N-hydroxyalkylphthalimide, sn-
7. glycero-S-phosphoryl-N-(2-hydroxyethyl)phthalimide IV; R =H, n-=1 and Z phthalimido, 2.8 g., prepared as described in Example 1, in 20 ml. of dimethylformamide, is added 5.0 g. of the freshly prepared acyl chloride octadeca-9,12,15-trienoyl chloride, and 2.7 ml. of dry pyridine. The solution is stored under nitrogen at 50 for 48 hours. It is poured into ice and 2 N sulfuric acid. The separated oil is extracted with ether. The ether extract is Washed with cold Water and dried over sodium sulfate. Removal of solvent gives an oil. The oil is subjected to chromatography using a silicic acid column (40 x 4 cm.). Elution with petroleum ether, benzene mixtures (1:1 and 1:3) and benzene gives compounds showing a negative phosphate test. Elution .of the column with 5% methanol in benzene affords the desired phosphatidyl-N-hydroxyal-kylphthalimide, 1,2 di (octadeca-9,12,15-trienoyl)-snglycero-3-phosphoryl-N-(2-hydroxyethyl)phthalimide (V; R =H, n=1 and Z=phthalirnido),
.ggq 3400-3250 1775, 1740 and 1720 cmr In the same manner, but using the acid chlorides, octadeca-6,9,12-trienoyl chloride, octadeca-6,9,12,15-tetraenoyl chloride, eicosa-8,11,l4-trienoyl chloride or eicosa- 5,8,11,14-tetraenoyl chloride instead of octadeca-9,12,l5- trienoyl chloride, the phosphatidyl-N-hydroxyalkylphthalimides,1,2 di (octadeca-6,12,15-trienoyl)-, 1,2-di-(octa- 6,9,12,15-tetraenoyl)-, di-(eicosa-8,11,14-trienoyl)- and di (eicosa 5,8,11,14 tetraenoyl-sn-glycero-3-phosphory1)-N-(Z-hydroxyethyl)-phthalimides, are obtained, respectively.
In the same manner, but using the acid chlorides, octadeca-6,9,12-trienoyl chloride, octadeca-9,12,15-trienoyl chloride, octadeca-6,9,12,lS-tetraenoyl chloride, eicosa- 8,11,14-trienoyl chloride or eicsa-5,8,11,14-tetraenoyl chloride and the appropriate glycerophosphoryl-N-hydroxyalkylphthalimides, prepared asdescribed in Example 1, the corresponding, phosphatidyl-N-hydroxyalkylphthalimides, 1,2-di-(octadeca-6,9,l2-trienoyl), 1,2-di- (octadeca 9,12,15 trienoyl), 1,2-di-(octadeca-6,9,12,15- tetraenoyl), 1,2-di-(eicosa-8,11,14-trienoyl), 1,2-di-(eicosa- 5,8,11,14-tetraenoyl) esters of glycerophosphoryl-N-hydroxyalkylphthalimides, sn-glycero-3-phosphoryl-N-(3-hydr0xypropyl)-, -N-(2-hydroxypropyl)-, -N-(3-hydroxybutyl)-phthalimides, are obtained.
In a like manner, the manipulative procedure described in this example may be employed using an appropriate acid chloride, described above, and glycerophosphoryl- N-hydroxyalkylphthalimide, prepared as described in Example l, in a molar ratio of 0.8:1, followed by a repetition of said manipulative procedure using a three molar excess of a different acid chloride, described above, relative to the amount of said glycerophosphoryl-N-hydroxyalkylphthalimide, to yield the mixed diacylated phosphatidyl-N-hydroxyalkylphthalimides, 1-(octadeca-6,9,12-trienoyl) 2 (octadeca-9,12,15-trienoyl)-, -2-(octadeca-6,9, 12,l-tetraenoyl)-, -2-(eicosa-8,11,14-trien0yl)-, -2-(eicosa-5,8,l1,14-tetraenoyl)-; 1-(octadeca-9,12,15-trienoyl)-2- (octadeca-6,9,12-trienoyl)-, -2-(octadeca 6,9,12,15-tetraenoyl)-, 2- (eicosa-8,1 1,14-trienoyl) 2- (eicosa-5,8,1 1, l4-tetraenoyl)-; 1 (octadeca 6,9,12,15 tetraenoyl)-2- (octadeca-6,9,12-trienoyl)-, -2-(octadeca 9,12,15 trienoyl)-, -2-(eic0sa-8,11,l4-trienoyl)-, -2-(eicosa-5,8,l1,14- tetraenoyl)-; 1-(eic0sa-8,11,14trienoyl)-2-(octadeca-6,9, 12-trienoy1) -2-(octadeca-9,12,15-trienoyl)-, -2- (octadeca-6,9,12,15-tetraenoyl)-, '-2-(eicosa-5,8,11,14 tetraenoyl)-; 1-(eicosa-5,8,11,14-tetraenoyl)-2-(octadeca-6,9,12- trienoyl)-, -2-(octadeca-9,12,15-trienoyl)-, 2-(octadeca6, 9,12,15-tetraenoyl) 2-(eicosa-8,l1,14-trienoyl)-sn-glycero-3-phosphoryl-N- (Z-hydroxyethyl -N- 3-hydroxypropyl)-, -N-(2-hydroxypropyl)- and -N-(3-hydroxybutyl) phthalimides.
In the same manner, but using the corresponding racg1ycerophosph0ry1-N-hydroxyalkylphthalimide, prepared as described in Example 1 instead of the sn-glycerophosphoryl-N-hydroxyalkylphthalimides, employed above in this example, the corresponding rac-phosphatidyl-N-hydroxyal'kylphthalimides described in this example, are obtained.
EXAMPLE 3 To a solution of 2.5 g. of the sn-phosphatidyl-N-hydroxyalkylphthalimide, 1,2-di-(octadeca-9,12,15-trienoyl)- sn-glycero 3 phosphoryl N (2 hydroxyethyl)phthalimide, prepared as described in Example 2, in 60 ml. of dry ethanol at 0, 1.25 ml. of a 12.5% hydrazine hydrate solution is added and kept at said temperature for 30 min. Another 1.8 m1. of the same hydrazine solution is added and the mixture is boiled under nitrogen for 2 hours. The solvent is removed under reduced pressure and the residue extracted with ether. The ether extract is mixed with 5 ml. of methanol and 5 ml. of Water and the mixture shaken With 2 g. of Dowex 50 (I-I resin for one hour. The mixture is filtered and the filtrate is concentrated to dryness under reduced pressure. The residue is subjected to chromatography on a column of silicic acid (4 x 30 cm.). The column is exhaustively eluted, first With benzene-chloroform (1:2) and then with chloroform-methanol (9:1). The chloroform-methanol eluates are concentrated to yield the sn-phosphatidylethanolamine, 1,2-di-(octadeca- 9,12,15 trienoyl) sn-glycero-3-phosphorylethanolamine,
1735, 1650 and 1245 cmr In the same manner, but using equivalent amounts of the other sn-phosphatidyl-N-hydroxyalkylphthalirnides, prepared as described in Example 2, instead of 1,2-di- (octadeca 9,12,15 trienoyl) sn glycero 3 phosphoryl-N-(2-hydroxyethyl phthalamide, the corresponding phosphatidylethanolamines, 1,2 di (octadeca- 6,9,12 trienoyl)-, 1,2 di (octadeca 6,9,12,15-tetraenoyl)-, 1,2 di (eicosa 8,11,14 trienoyl)- and 1,2- di (eicosa 5,8,11,14 tetraenoyl) sn glycero 3- phosphorylethanolamines, are obtained. Similarly, the corresponding sn phosphatidylethanolamine derivatives, 1,2 di (octadeca 6,9,12 trienoyl), 1,2 di (octadeca 9,12,15 trienoyl), 1,2 di (octadeca 6,9,12,15- tetraenoyl), 1,2 di (eicosa 8,11,14 trienoyl), 1,2- di (eicosa 5,8,11,14 tetraenoyl) esters of sn glycero- 3 phosphoryl (3 hydroxypropylamine), -(2 hydroxypropylamine), and -(3 hydroxybutylamine), are obtained. Similarly, the corresponding mixed acylated snphosphatidylethanolamine derivatives, 1 (octadeca- 6,9,12 trienoyl) 2 (octadeca 9,12,15 trienoyl)-, -2 (octadeca 6,9,12,15 tetraenoyl)-, -2 (eicosa- 8,11,14 trienoyl)-, -2 (eicosa 5,8,11,14 tetraenoyl)-; 1 (octadeca 9,12,15 trienoyl) 2 (octadeca 6,9,12- trienoyl)-, -2 (octadeca 6,9,12,15 tetraenoyl)-, -2- (eicosa 8,11,14 trienoyl)-, -2 (eicosa 5,8,11,14- tetraenoyl)-; 1 (octadeca 6,9,12,15 tetraenoyl) 2- (octadeca 6,9,12,15 trienoyl)-, -2 (octadeca 9,12,15- trienoyl)-, -2 (eicosa 8,11,14 trienoyl)-, -2 (eicosa- 5,8,11,14 tetraenoyl)-; 1 (eicosa 8,11,14 trienoyl)- 2 (octadeca 6,9,12 trienoyl)-, -2 (octadeca 9,12,15- trienoyl)-, -2 (octadeca 6,9,12,15 tetraenoyl)-, -2- eicosa 5,8,11,14 tetraenoyl)-; 1 (eicosa 5,8,11,14- tetraenoyl) 2 (octadeca 6,9,12 trienoyl) -2 (octadeca 9,12,15 trienoy1)-, 2 (octadeca 6,9,12,15- tetraenoyl)-, -2 (eicosa 8,11,14 trienoyl) sn glycero- 3 phosphoryl 2 ethanolamines, -3 hydroxypropylamines, -2 hydroxypropylamines and -3 hydroxybutylamines, are obtained.
In the same manner, but using the corresponding racphosphatidyl-N-hydroxyalkylphthalimides, prepared as described in Example 2 instead of the sn-phosphatidyl- N-hydroxyalkylphthalimides employed above in this example, the corresponding rac-phosphatidylethanolamine derivatives are obtained.
9 EXAMPLE 4 To a stirred solution of 45.1 g. of di(2,2,2-trichloroethyl)phosphorochloridate (VI) in 300 ml. of dry pyridine at C., 12.6 g. of 1,2 isopropylidine sn glycerol (II) in 50 ml. of pyridine is added dropwise over a period of 30 minutes. The reaction mixture is kept at 0 C. for 12 hours and then concentrated under reduced pressure. The residue is dissolved in chloroform and the resultant solution washed with dilute sodium bicarbonate. Concentration of the chloroform solution yields 1,2-isopropylidine sn glycero 3 phosphoric acid di (2,2,2- trichloroethyl)-ester (VII),
6 9 1.35 (s, 3 protons) 1.45 (s, 3 protons) and 4.75 (s, 4 protons).
In the same manner, but using an equivalent amount of rac 1,2 isopropylidene glycerol instead of 1,2 isopropylidene sn glycerol, rac 1,2 isopylidene glycerol- 3 phosphoric acid di (2,2,2 trichloroethyl)ester is obtained.
EXAMPLE 5 A solution of 5.0 g. of 1,2 isopropylidene sn glycero 3 phosphoric acid di (2,2,2 trichloroethyl)- ester (VII), prepared as described in Example 4, in 250 ml. of 80% methanol is passed through a column of Dowex 50 (H+). The column is rinsed with one liter of 80% methanol. The combined elfiuents are evaporated at reduced pressure at 35-40 C., yielding sn glycero 3- phosphoric acid di (2,2,2 trichloroethyl) ester (VIII),
CHOlz max.
3600 cm.- (broad), 6
EXAMPLE 6 By using the manipulative procedures and the appropriate acid chlorides, described in Example 2, but substituting an equivalent amount of snor rac-glycero- 3-phosphroic acid di-(2,2,2-trichloroethyl)-ester, prepared as described in Example 5 in place of the glycerophosphoryl N hydroxylalkylphthalimide, followed by treatment with an excess of zinc dust in 80% aqueous acetic acid for one hour at room temperature to remove the 2,2,2-trichloroethyl ester groups the corresponding snor racphosphatidic acid derivatives, 1,2 di (octadeca 6,9,12- trienoyl)-, 1,2 di (octadeca 9,12,15 trienoyl)-, 1,2- di (octadeca 6,9,12,15 tetraenoyl)-, 1,2 di (eicosa- 8,11,14 trienoyl)-, 1,2 di (eicosa 5,8,11,14 tetraenoyl)-; 1 (octadeca 6,9,12 trienoyl) 2 (octadeca- 9,12,15 trienoyl)-, -2 (octadeca 6,9,12,15 tetraenoyl)-, -2 (eicosa 8,11,14 trienoyl)-, -2 (eicosa- 5,8,1l,14 tetraenoyl)-; 1 (octadeca 9,12,15 trienoyl) 2 (octadeca 6,9,12 trienoyl)-, -2 (octadeca- 6,9,12,15 tetraenoyl)-, -2 (eicosa 8,11,14 trienoyl)-, -2 (eicosa 5,8,11,14 tetraenoyl)-; 1 (octadeca- 6,9,12,15 tetraenoyl) 2 (octadeca 6,9,12 trienoyl)-, -2 (octadeca 9,12,15 trienoyl)-, -2 (eicosa 8,11,14- trienoyl)-, -2 (eicosa 5,8,11,14 tetraenoyl)-; l-(eicosa- 8,11,14 trienoyl) 2 (octadeca 6,9,12 trienoyl)-, -2 (octadeca 9,12,15 trienoyl)-, -2 (octadeca 6,9,12,15 tetraenoyl)-, -2 (eicosa 5,8,11,14 tetraenoyl)-; 1 (eicosa 5,8,11,14 tetraenoyl) 2 (octadeca 6,9,12 trienoyl)-, -2 (octadeca 9,12,15 trienoyl)-, 2 (octadeca 6,9,12,15 tetraenoyl)-, -2- (eicosa 8,11,14 trienoyl)-, glycero 3 phosphoric acids, are obtained.
The 1,2 di (octadeca 9,12,15 trienoyl) snglycero 3 phosphoric acid (X; R and R =CH (CH CH=CH)3(CH COO) has 173 59 1750 cm.- (broad) EXAMPLE 7 A solution of 7.0 g. of the phosphatidic acid derivative 1,2 di-(octadeca 9,12,15 trienoyl)-sn-glycero-3-phosphoric acid, prepared as described in Example 5, 1.8 g. of the hydroxyalkylphthalimide, N-(2 hydroxyethyl)phtha1- imide, and 5.00 g. of trichloroacetonitrile, described by W. Steinkopf, Chem. Ber., 41, 2540 (1908), in 40 ml. of pyridine is heated at -100 C. for four hours. The reaction mixture is diluted with water (300 ml.) adjusted to pH 6 with concentrated hydrochloric acid, mixed with ice, and extracted with chloroform. The chloroform extract is dried over sodium sulfate, filtered and evaporated to dryness. The residual oil is subjected to chromatography using a silicic acid column (40X 8 cm.). After elution of the column with petroleum ether, petroleum ether-benzene (1:1) and benzene, elution with 5% methanol in benzene affords 1,2 di (octadeca 9,12,15 trienoyl)-sn-glycero- 3-phosphoric acid,
vCHCh 3400-3250 In the same manner, but using the appropriate snor rac-phosphatidic acid derivative, prepared as described in Example 6, and the appropriate 2- or 3-hydroxyalkylphthalimide, described above, the corresponding snor rac-phosphatidyl-N hydroxyalkylphthalimides, described in Example 2, are also obtained.
EXAMPLE 8 A solution of 1.0 g. of (2,2,2-trichloroethoxy)carbonyl chloride (X1) in 5 ml. of dry ether is added slowly to a vigorously stirred ice-cold solution of 500 mg. of 1,2-isopropylidine-sn-glycerol (II) and 1 ml. of pyridine in 5 ml. of dry ether. After stirring at room temperature for one hour, the reaction mixture is diluted with ether. The resultant precipitate of pyridine hydrochloride is removed by filtration. The filtrate is washed successively with cold 2 N-hydrochloric acid and water, dried over sodium sulfate, filtered and evaporated to dryness. The oily product, 1,2 isopropylidine-sn-glycero 3 carbonic acid (2,2,2- trichloroethyl) -ester (XII),
H l D .35 1750 cm. ag f 1.35 (s, 3 protons), 1.45 (s, 3 protons), 4.75 (s, 2 protons) is not purified further but used for the next step described in Example 9.
In the same manner, but using an equivalent amount of rac 1,2 isopropylidine-glycerol instead of 1,2 isopropylidine-sn-glycerol, rac 1,2 isopropylidine-glycero-3- carbonic acid (2,2,2-trichloroethyl)ester is obtained.
EXAMPLE 9 To a solution of 1.1 g. of 1,2 isopropylidine-sn-glycero- 3 carbonic acid (2,2,2 trichloroethyl)-ester (XII), prepared as described in Example 8, in 20 ml. of methanol, 0.1 ml. of concentrated hydrochloric acid is added. The resultant solution is kept at room temperature for one hour and then evaporated to dryness under reduced pressure. After drying in high vacuum for 24 hours over sodium hydroxide, the oily residue, sn-glycero-S-carbonic acid (2,2,2 trichloroethyl)-ester (XIII),
11,9 ,39 3500 and 1750 emf- 52 35 4.75 (s, 2 protons) By using the manipulative procedures and the appropriate acid chlorides, described in Example 2, but substituting an equivalent amount of snor rac-glycero 3 carbonic acid (2,2,2 trichloroethyl)-ester, prepared as described in Example 9 in place of the glycerophosphoryl-N-hydroxyalkylphthalimide, followed by treatment with an excess of zinc dust in glacial acetic acid for one hour at room temperature to remove the 2,2,2-trichlorethyl ester group the corresponding snor rac-glycerol diacylate derivatives, 1,2 di-(octadeca-6,9,12 trienoyl)-, 1,2 di- (octadeca 9,12,15 trienoyl)-, 1,2 di-(octadeca 6,9, 12,15 tetraenoyl)-, 1,2 di-(eiscosa 8,11,14 trienoyl)-, 1,2 di-(eicosa 5,8,11,14 tetraenoyl)-; 1 (octadeca- 6,9,12 trienoyl) 2 (octadeca 9,12,15 trienoy1)-, -2- (octadeca 6,9,12,15 tetraenoyl)-, -2 (eicosa 8,11,14- trienoyl)-, -2-(eicosa-5,8,11,14-tetraenoyl)-; l-(octadeca- 9,12,15 trienoyl) 2 (octadeca 6,9,12 trienoy1)-, -2- (octadeca 6,9,12,15 tetraenoyl)-, -2-(eicosa 8,11,14- trienoyl)-, -2-(eicosa 5,8,11,14 tetraenoyl)-; l-(octadeca 6,9,12,15 tetraenoyl) -2 (octadeca 6,9,12-trienoyl)-, -2-(octadeca 9,12,15 trienoyl)-, -2-(eicosa 8, 11,14 trienoyl)-, -2(eicosa 5,8,11,14-tetraenoyl); 1- (eicosa 8,11,14 trienoyl) 2 (octadeca 6,9,10 trienoyl)-, -2-(octadeca-9,12,15-trienoyl)-, -2-(octadeca-6,9, 12,15-tetraenoyl)-, -2-(eicosa-5,8,11,14 tetraenoyl)-; 1- (eicosa 5,8,11,14 tetranoyl) 2 (octadeca 6,9,12- trienoyl)-, -2-(octadeca-9,12,15-trienoyl)-, 2-(octadeca-6, 9,12,15-tetraenoyl)-, -2-(eicosa 8,11,14,,- trienoyl), glycerols are obtained.
The 1,2 di-(octadeca 9,12,15 trienoyl)-sn-glycerol :(XV); :R and R =CH (CH -CH=CH) (CH COO has V359 3400 and 1745 cm.-
EXAMPLE 11 To a solution of 0.46 g. of freshly distilled phosphorous oxychloride in ml. of dry methylene chloride at 0 0,, is added during 30 min. a solution of 1,2 di-(octadeca- 9,12,15 trienoyl)-sn-glycerol, prepared as described in Example 10, and 0.43 g. of dry quinoline in 10 ml. of dry methylene dichloride. The reaction mixture is kept at room temperature for one hour. The temperature of the reaction mixture is again reduced to 0 C. and a solution of 0.48 g. of 2-hydroxyethylcarbamic acid tert-butyl ester and 0.8 g. of pyridine in 10 ml. of methylene chloride is added over a period of 30 min. After standing at room temperature for 2 hours, 0.05 ml. of water is added and the reaction mixture is stirred for one hour, diluted 'with 200 ml. of methylene chloride and washed successively with cold 2 N hydrochloric acid and water, dried over sodium sulfate, filters and concentrated under reduced pressure to yield an oil. The oil is purified by chromatography on silicic acid. Elution with 5% methanol in benzene affords the phosphatidyl derivative, 1,2-di-(octadeca-9,12,lS-trienoyl)-sn-glycero 3 phosphoryl-N-(tert-butyloxycarbonyl)ethanolamine (V); R and R =CH (CH CH CH 3 (CH COO R =H, n =1 and Z=NHCOOC(CH ,CHOla max.
3500-3400, 1745 and 1690 cmr This derivative is dissolved in ml. of dry ether and treated with a stream of dry hydrogen chloride at 0 C. for two hours. The solvent is then removed by under reduced pressure at 30 C. The oily residue is subjected to chromatography on g. of silicic acid. Elution with 510% methanol in chloroform yields the desired phosphatidylalkanolamine, 1,2-di-(octadeca 9,12,15 trienoyl)-sn-glycero 3 phosphorylethanolamine,
ase 3500-3100, 1735, 1650 and 1245 cmr identical with the product obtained in Example 3.
In the same manner, but using the appropriate snor rac-glycerol diacylate described in Example 10 instead of 1,2 di-(octadeca 9,12,15 trienoyl)-sn-glycerol together with the appropriate hydroxyalkylcarbamic acid tert-butyl ester, described above, instead of 2-hydroxyethylcarbamic acid tert-butyl ester, the remaining phosphatidylalkanolamines described in Example 3 are obtained.
I claim:
1. 1,2 di-(octadeca 9,12,15 trienoyl)-sn-glycero-3- phosphorylethanolamine.
References Cited UNITED STATES PATENTS 2,629,662 2/1953 Julian et al 260-403 2,864,848 12/ 1958 McArthur 260-403 3,189,628 6/1965 Knight et al. 260-403 ELBERT L. ROBERTS, Primary Examiner US. Cl. X.R.
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Cited By (22)
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US3681412A (en) * | 1969-09-30 | 1972-08-01 | Nattermann A & Cie | Glycol phosphatides and the preparation thereof from cephalin |
US3875196A (en) * | 1970-12-28 | 1975-04-01 | Ajinomoto Kk | Process for preparing phosphoric acid esters of fatty acid mono- or di-glycerides |
US3940423A (en) * | 1973-09-06 | 1976-02-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | 1,2-O-dialkylmethylidene-glycero-3-phosphatides |
US3985875A (en) * | 1974-01-12 | 1976-10-12 | Dainippon Pharmaceutical Co., Ltd. | ω-(N-acylamino)alkylphosphoryl ethanolamines, pharmaceutical compositions containing them, and their uses |
US4086257A (en) * | 1976-10-12 | 1978-04-25 | Sears Barry D | Phosphatidyl quaternary ammonium compounds |
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US4159988A (en) * | 1974-08-06 | 1979-07-03 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Synthetic phospholipids, a process for their manufacture and their use |
US4160773A (en) * | 1974-08-06 | 1979-07-10 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Synthetic alkyl esters of phospholipid acid, structural analogs thereof and a process for their manufacture and their use |
USRE30748E (en) * | 1976-10-12 | 1981-09-22 | Phosphatidyl quaternary ammonium compounds | |
US4312860A (en) * | 1980-10-24 | 1982-01-26 | Regents Of The University Of California | Lung surfactant compositions |
US4348329A (en) * | 1979-12-20 | 1982-09-07 | Dennis Chapman | Biocompatible surfaces |
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US4555506A (en) * | 1981-12-24 | 1985-11-26 | E. R. Squibb & Sons, Inc. | Phosphorus containing compounds and use as hypotensives |
US4742051A (en) * | 1977-06-30 | 1988-05-03 | University Of Tennessee Research Corporation | Antihypertensive compounds |
US4814112A (en) * | 1986-11-13 | 1989-03-21 | Cl Pharma Aktiengesellschaft | Single-stage process for preparing mixed-substituted 1,2-diacyl-sn-clycero-3-phosphocholines |
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US3681412A (en) * | 1969-09-30 | 1972-08-01 | Nattermann A & Cie | Glycol phosphatides and the preparation thereof from cephalin |
US3875196A (en) * | 1970-12-28 | 1975-04-01 | Ajinomoto Kk | Process for preparing phosphoric acid esters of fatty acid mono- or di-glycerides |
US3940423A (en) * | 1973-09-06 | 1976-02-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | 1,2-O-dialkylmethylidene-glycero-3-phosphatides |
US3985875A (en) * | 1974-01-12 | 1976-10-12 | Dainippon Pharmaceutical Co., Ltd. | ω-(N-acylamino)alkylphosphoryl ethanolamines, pharmaceutical compositions containing them, and their uses |
US4159988A (en) * | 1974-08-06 | 1979-07-03 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Synthetic phospholipids, a process for their manufacture and their use |
US4160773A (en) * | 1974-08-06 | 1979-07-10 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Synthetic alkyl esters of phospholipid acid, structural analogs thereof and a process for their manufacture and their use |
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US4097502A (en) * | 1976-10-12 | 1978-06-27 | Sears Barry D | Phosphatidyl sulfonium hydroxide compounds |
USRE30748E (en) * | 1976-10-12 | 1981-09-22 | Phosphatidyl quaternary ammonium compounds | |
US4742051A (en) * | 1977-06-30 | 1988-05-03 | University Of Tennessee Research Corporation | Antihypertensive compounds |
US4348329A (en) * | 1979-12-20 | 1982-09-07 | Dennis Chapman | Biocompatible surfaces |
US4933114A (en) * | 1980-08-11 | 1990-06-12 | Eastman Kodak Company | Polyacetylenic lipids, radiation-sensitive compositions, photographic elements and processes relating to same |
US4312860A (en) * | 1980-10-24 | 1982-01-26 | Regents Of The University Of California | Lung surfactant compositions |
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US4493832A (en) * | 1981-07-03 | 1985-01-15 | Fujisawa Pharmaceutical Co., Ltd. | Certain glycerol-phosphoryl choline derivatives, compositions containing same and method of using same |
US4534899A (en) * | 1981-07-20 | 1985-08-13 | Lipid Specialties, Inc. | Synthetic phospholipid compounds |
US4555506A (en) * | 1981-12-24 | 1985-11-26 | E. R. Squibb & Sons, Inc. | Phosphorus containing compounds and use as hypotensives |
US4814112A (en) * | 1986-11-13 | 1989-03-21 | Cl Pharma Aktiengesellschaft | Single-stage process for preparing mixed-substituted 1,2-diacyl-sn-clycero-3-phosphocholines |
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US5466841A (en) * | 1993-01-27 | 1995-11-14 | Scotia Holdings Plc | Formulations containing unsaturated fatty acids |
WO1994027580A1 (en) * | 1993-05-21 | 1994-12-08 | The Liposome Company, Inc. | Reduction of liposome-induced adverse physiological reactions |
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