US3607094A - Apparatus for pipetting and adding a liquid - Google Patents

Apparatus for pipetting and adding a liquid Download PDF

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US3607094A
US3607094A US793990*A US3607094DA US3607094A US 3607094 A US3607094 A US 3607094A US 3607094D A US3607094D A US 3607094DA US 3607094 A US3607094 A US 3607094A
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pump
piston
cylinder
actuating
liquid sample
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US793990*A
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Palle-Finn Beer
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Autokemi AB
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Autokemi AB
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B01L3/0206—Burettes, i.e. for withdrawing and redistributing liquids through different conduits of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Definitions

  • the present invention relates to an arrangement in a pump for p I; etting a liquid sample and for adding a liquid reagent.
  • the invention is especially adapted for use in micropipetting, ie when the pipetting takes place with volumes smaller than 15 microliters, but it is not restricted to this application and can be used with advantage when it is a question of pipetting very accurately determined volumes.
  • a pump comprising a pump cylinder, a pump piston insertable in said pump cylinder, and actuation means for said pump piston, a tubular cannula connected to said pump cylinder for drawing up a liquid sample, a conduit for supplying liquid reagent to said pump cylinder, said conduit having provided therein a valve means adapted to be actuated to its open position when the pump piston reaches a position corresponding to an emptying of the pump cylinder.
  • the valve means provided in the conduit preferably comprises a valve element held in closed position by spring means. Opening of the valve means may be achieved by employing as the piston pump one having the form of a rod-shaped displacement piston and adapting the piston rod to lift the valve element from its closed position when the pump rod reaches the position corresponding to an emptying of the pump cylinder.
  • the actuating means for the pump piston is preferably com posed of an actuating piston slidably disposed and springloaded in a housing, the actuating piston being connected to the pump piston, and means for supplying compressed air to drive the actuating piston.
  • the compressed air acts on one side of actuating piston 10 and on the other side thereof a constant pressure is maintained through an aperture 18 provided in the wall of the cylinder.
  • the pump cylinder 8 communicates directly with the tubular cannula 6 and is also connected through a valve 19 with a conduit 20 for supplying liquid reagent from container 5.
  • This conduit also has provided in it a pump 21 for the liquid reagent and a valve means 22 which is controlled by control means 15.
  • valve member 23 of the valve 19 is held by a spring 24 in seated position and can be unseated against the action of spring 24 by the pump piston 9 pushing against valve member 23 in the position of the pump piston corresponding to a complete emptying of the pump cylinder.
  • cannula 6 With the pump piston 9 in the uppermost position with reference to the drawing, cannula 6 is lowered into test tube 3 after which valve means 14 is actuated by control means 15 to a position in which the compressed air does not act on actuating piston 10. The latter is then brought by spring 12 to the lowermost position with reference to the drawing as defined by rod 16. This causes a predetermined quantity of liquid sample to be drawn into cannula 6 corresponding to the displacement of piston 9, and during this movement of the piston valve 19 remains closed whereby the quantity of liquid sample that is drawn into cannula 6 is determined exclusively by the displacement of the piston in cylinder 8.
  • Cannula 6 is then moved along the path shown in dotted lines to test tube 4 and valve means 14 is actuated by control means 15 so as to cause compressed air again to be supplied to the pump.
  • Actuating piston 10 with pump piston 9 are then displaced against the action of spring 12 to the uppermost position and the quantity of liquid sample drawn into cannula 6 is emptied into test tube 4.
  • control means 15 actuates the other valve means 22 causing the connection between liquid reagent pump 21 and conduit 20 to be established.
  • An apparatus for pipetting a liquid sample and supplying a liquid reagent which comprises a pump cylinder, a pump piston insertable into said pump cylinder, a tubular cannula connected to said pump cylinder, actuating means for displacing said pump piston from said pump cylinder to draw liquid sample from a source and for inserting said pump piston into said pump cylinder to discharge said drawn liquid sample to a location other than said source, means for moving said tubular cannula to said other location, a conduit for supplying liquid reagent to said pump cylinder for delivery through said tubular cannula to said liquid sample location subsequent to discharge of said liquid sample, said conduit being provided with a valve means, said valve means being in closed position during said drawing and discharge of liquid sample and in open position upon discharge of said liquid sample, opening of said valve means being effected by said pump piston upon insertion through said chamber, whereby the reagent can enter said pump cylinder.
  • valve means comprises a valve element held in closed position by spring means and the pump piston has the form of a rod-shaped displacement piston for lifting said valve element from said closed position.
  • actuating means comprises an actuating piston slidably disposed and springloaded in a housing, said actuating piston being connected to said pump, and means for supplying compressed air to drive said actuating piston.

Abstract

An apparatus for pipetting a liquid sample and supplying a liquid reagent which comprises a pump cylinder, a pump piston insertable in said pump cylinder, actuating means for said pump piston, a tubular cannula connected to said pump cylinder for drawing up a liquid sample, a conduit for supplying liquid reagent to said pump cylinder, said conduit having provided therein a valve means adapted to be actuated to its open position when the pump piston reaches a position corresponding to an emptying of the pump cylinder.

Description

United States Patent Inventor Palle-Finn Beer Lidingo, Sweden Appl. No. 793,990 Filed Jan. 27, 1969 Patented Sept. 21, 1971 Assignee AB Autokemi Stockholm, Sweden Priority June 25, 1968 Sweden 1020/68 APPARATUS FOR PIPE'I'IING AND ADDING A LIQUID 3 Claims, 1 Drawing Fig.
11.8. C1 23/253, 23/259,73/425.6,141/25,141/l05,141/130, 222/207, 417/443, 417/446, 417/503 Int. Cl B011 11/00, G01n1/14,G01n 31/00 Field of Search 23/253,
259, 292; 141/25, 105, 130; 73/4256; ZZZ/129.1, 129.3, 133, 207; 417/443, 446, 503
[56] I References Cited UNITED STATES PATENTS 1,896,134 2/1933 Clark 222/1293 X 2,216,890 10/1940 Philipps 222/133 3,193,359 7/1965 Baruch et a1. 23/253 X 3,421,858 1/1969 Quinn 23/259 X 3,475,130 10/1969 Baruch 23/253 Primary Examiner-Joseph Scovronek Attorney-Larson, Taylor and Hinds ABSTRACT: An apparatus for pipetting a liquid sample and supplying a liquid reagent which comprises a pump cylinder, a pump piston insertable in said pump cylinder, actuating means for said pump piston, a tubular cannula connected to said pump cylinder for drawing up a liquid sample, a conduit for supplying liquid reagent to said pump cylinder, said conduit having provided therein a valve means adapted to be actuated to its open position when the pump piston reaches a position corresponding to an emptying of the pump cylinder.
APPARATUS FOR PIPETTING AND ADDING A LIQUID BACKGROUND OF THE INVENTION The present invention relates to an arrangement in a pump for p I; etting a liquid sample and for adding a liquid reagent.
The invention is especially adapted for use in micropipetting, ie when the pipetting takes place with volumes smaller than 15 microliters, but it is not restricted to this application and can be used with advantage when it is a question of pipetting very accurately determined volumes.
Particularly in micropipetting with the addition of a liquid reagent it has proved difficult to achieve a constant pipetted volume of liquid sample from one time to the next, which is partly due to variations in volume of the flexible tube serving for applying liquid reagent to the pipette. For example, the elasticity of the tube and the variations in the time required for it to return to its original volume after a stroke of the pump leads to important variations in the overall volume owing to temperature variations, hysteresis in the material of the tube, and movement of the tube. These variations can be as high as several percent.
SUMMARY OF THE INVENTION These difiiculties are avoided according to the present invention by means of a pump comprising a pump cylinder, a pump piston insertable in said pump cylinder, and actuation means for said pump piston, a tubular cannula connected to said pump cylinder for drawing up a liquid sample, a conduit for supplying liquid reagent to said pump cylinder, said conduit having provided therein a valve means adapted to be actuated to its open position when the pump piston reaches a position corresponding to an emptying of the pump cylinder.
The valve means provided in the conduit preferably comprises a valve element held in closed position by spring means. Opening of the valve means may be achieved by employing as the piston pump one having the form of a rod-shaped displacement piston and adapting the piston rod to lift the valve element from its closed position when the pump rod reaches the position corresponding to an emptying of the pump cylinder.
The actuating means for the pump piston is preferably com posed of an actuating piston slidably disposed and springloaded in a housing, the actuating piston being connected to the pump piston, and means for supplying compressed air to drive the actuating piston.
BRIEF DESCRIPTION OF THE DRAWING The invention will be described below with reference to the attached drawing showing an embodiment of the pipetting apparatus.
DESCRIPTION OF THE PREFERRED EMBODIMENT The apparatus for the invention shown in the FIGURE is adapted to transfer liquid sample from a test tube in a series 1 of test tubes to another test tube in another series 2 of test tubes and for supplying liquid reagent to the quantity of liquid sample thus transferred. For instance, a certain quantity of liquid sample is to be transferred from the test tube 3 of the first series to the test tube 4 of the second series and liquid reagent is to be supplied from a container 5. To this end, there is provided in the apparatus a tubular cannula 6 which can be moved in a path indicated by the dotted line 7 in a manner known per so, as shown on the drawing. Cannula 6 is connected to a pump comprising a cylinder 8 with a pump piston 9 insertable therein and preferably having the form of a rodshaped displacement piston. The pump piston is connected to an actuating piston 10 provided in a cylinder 11. A spring 12 urges the actuating piston 10 towards an end position in cylinder 1 l. Actuating piston 10 can be brought into the other terminal position against the action of spring 12 by means of compressed air supplied from a compressed air source 13. The supply of compressed air to cylinder 11 is controlled by a valve means 14 which responds to a common control means 15 provided for the apparatus. The stroke of actuating piston 10 can be adjusted by means of a rod 16 which can be screwed to a variable degree into an end wall of cylinder 11. The position of rod 16 can be fixed with a lock nut 17 which may also serve as a sealing between rod 16 and the end wall of the cylinder.
The compressed air acts on one side of actuating piston 10 and on the other side thereof a constant pressure is maintained through an aperture 18 provided in the wall of the cylinder.
The pump cylinder 8 communicates directly with the tubular cannula 6 and is also connected through a valve 19 with a conduit 20 for supplying liquid reagent from container 5. This conduit also has provided in it a pump 21 for the liquid reagent and a valve means 22 which is controlled by control means 15.
The valve member 23 of the valve 19 is held by a spring 24 in seated position and can be unseated against the action of spring 24 by the pump piston 9 pushing against valve member 23 in the position of the pump piston corresponding to a complete emptying of the pump cylinder.
The operation of the apparatus will now be described. First, however, it should be noted that the test tubes of series 1 and 2 are not drawn to the same scale as the pump which has been enlarged on the drawing in the interest of clarity. Also, the entire unit comprising pump 8, 9 and actuating pump 10, 11 is adapted for movement together with tubular cannula 6 so that the latter can be attached to pump 8, 9 in a fixed manner. On the other hand, flexible portions 25 are provided in the connection between valve 14 and actuating cylinder 11 as well as between valve 22 and valve 19.
With the pump piston 9 in the uppermost position with reference to the drawing, cannula 6 is lowered into test tube 3 after which valve means 14 is actuated by control means 15 to a position in which the compressed air does not act on actuating piston 10. The latter is then brought by spring 12 to the lowermost position with reference to the drawing as defined by rod 16. This causes a predetermined quantity of liquid sample to be drawn into cannula 6 corresponding to the displacement of piston 9, and during this movement of the piston valve 19 remains closed whereby the quantity of liquid sample that is drawn into cannula 6 is determined exclusively by the displacement of the piston in cylinder 8. Cannula 6 is then moved along the path shown in dotted lines to test tube 4 and valve means 14 is actuated by control means 15 so as to cause compressed air again to be supplied to the pump. Actuating piston 10 with pump piston 9 are then displaced against the action of spring 12 to the uppermost position and the quantity of liquid sample drawn into cannula 6 is emptied into test tube 4. When the pump piston 9 has reached the topmost position, with reference to the drawing, it reached valve member 23 and causes it to be unseated. Simultaneously, or after this, control means 15 actuates the other valve means 22 causing the connection between liquid reagent pump 21 and conduit 20 to be established. Pump 21 is now made to supply a predetermined quantity of liquid reagent which flows through conduit 20 and valve 19 down into cylinder 8 and tubular cannula 6. Simultaneously, with the supply of liquid reagent to the liquid sample in test tube 4, the interior of cannula 6 is rinsed from possibly remaining traces of liquid sample. Cannula 6 is then transferred to the other test tube in series 1 after which the sequence of events just described is repeated.
To reduce still further the danger of an undesired transfer of liquid sample from one sample to another, it is possible, simultaneously with the supply of liquid reagent in the manner referred to above, to cause a rinsing of the exterior of cannula 6 by supplying liquid reagent via a branch conduit 26 of conduit 20 to an annular nozzle 27 placed around cannula 6. By
suitable dimensioning of branch conduit 27 relative to the path leading through cannula 6, it can be achieved that a suitable quantity of liquid reagent causes the desired rinsing of the exterior of cannula 6.
in the embodiment of the invention shown, movement of actuating piston in one sense is limited and adjusted by means of rod 16. Movement in the other sense is limited by means of the interior surface of the terminal wall of the piston contacting the free end of the tube 28 which is screwed into the upper end wall of cylinder 11. The length of tube 28 is selected so as to cause pump piston 9 to unseat valve member 23 at the precise moment when actuating piston 10 is stopped by tube 28. As indicated in the drawing, the upper end of the tube is arranged to hold a sealing O-ring in contact with pump piston 9.
It is claimed:
1. An apparatus for pipetting a liquid sample and supplying a liquid reagent which comprises a pump cylinder, a pump piston insertable into said pump cylinder, a tubular cannula connected to said pump cylinder, actuating means for displacing said pump piston from said pump cylinder to draw liquid sample from a source and for inserting said pump piston into said pump cylinder to discharge said drawn liquid sample to a location other than said source, means for moving said tubular cannula to said other location, a conduit for supplying liquid reagent to said pump cylinder for delivery through said tubular cannula to said liquid sample location subsequent to discharge of said liquid sample, said conduit being provided with a valve means, said valve means being in closed position during said drawing and discharge of liquid sample and in open position upon discharge of said liquid sample, opening of said valve means being effected by said pump piston upon insertion through said chamber, whereby the reagent can enter said pump cylinder.
2. The apparatus of claim 1 wherein the valve means comprises a valve element held in closed position by spring means and the pump piston has the form of a rod-shaped displacement piston for lifting said valve element from said closed position.
3. The apparatus of claim 1 wherein the actuating means comprises an actuating piston slidably disposed and springloaded in a housing, said actuating piston being connected to said pump, and means for supplying compressed air to drive said actuating piston.

Claims (2)

  1. 2. The apparatus of claim 1 wherein the valve means comprises a valve element held in closed position by spring means and the pump piston has the form of a rod-shaped displacement piston for lifting said valve element from said closed position.
  2. 3. The apparatus of claim 1 wherein the actuating means comprises an actuating piston slidably disposed and spring-loaded in a housing, said actuating piston being connected to said pump, and means for supplying compressed air to drive said actuating piston.
US793990*A 1968-01-25 1969-01-27 Apparatus for pipetting and adding a liquid Expired - Lifetime US3607094A (en)

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JP (1) JPS4947477B1 (en)
DE (1) DE1902297B2 (en)
FR (1) FR2000695A1 (en)
GB (1) GB1256746A (en)
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SE (1) SE327840B (en)

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3699348A (en) * 1970-08-14 1972-10-17 Zeiss Stiftung Radiation sensitive device for the successive investigation of several samples
US3735902A (en) * 1971-07-22 1973-05-29 Instrumentation Labor Inc Dispenser apparatus
US3739821A (en) * 1971-04-26 1973-06-19 Technicon Instr Machine-transferrable pipette
US3767364A (en) * 1971-07-30 1973-10-23 Sherwood Medical Ind Inc Reagent pipette system
US3827304A (en) * 1971-07-20 1974-08-06 Gilson W Sample handling method
DE2622691A1 (en) * 1976-05-21 1977-12-01 Technicon Instr Sample analyser with suction probe - can be immersed in liq. to suck it in, withdrawn and rinsed in top position before immersion in next sample
DE3015051A1 (en) * 1979-04-19 1980-10-23 Olympus Optical Co METHOD FOR DELIVERING SAMPLES AND REAGENTS IN REACTION TUBES
DE3033680A1 (en) * 1979-09-10 1981-03-12 Olympus Optical Co., Ltd., Tokyo DOSING AND LINE SYSTEM FOR ANALYZER DEVICES
US4318884A (en) * 1979-12-07 1982-03-09 Olympus Optical Co., Ltd. Distributing nozzle with cooperating ionic detector
US4340153A (en) * 1980-11-28 1982-07-20 Spivey David L Method and apparatus for medication dispensing
US4399711A (en) * 1980-04-18 1983-08-23 Beckman Instruments, Inc. Method and apparatus ensuring full volume pickup in an automated pipette
US4727033A (en) * 1981-12-17 1988-02-23 Olympus Optical Co., Ltd. Analyzing apparatus and method for immunological agglutination reactions
WO1990008307A1 (en) * 1988-12-29 1990-07-26 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5158748A (en) * 1990-01-18 1992-10-27 Mochida Pharmaceutical Co., Ltd. Automated dispensing and diluting system
US5364240A (en) * 1993-10-14 1994-11-15 Spartan Tool Div. Of Pettibone Corp. Fluid pump with pulsing feature
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5841039A (en) * 1991-04-04 1998-11-24 Technicon Instruments Corp. Apparatus and method for integrated sampling from closed and open sample containers
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US6302168B1 (en) * 2000-07-07 2001-10-16 Hugo Hu Automated precision liquid metering apparatus using injectors as metering devices
US20010034067A1 (en) * 2001-01-26 2001-10-25 Dales G. Cameron Apparatus and methods for parallel processing of multiple reaction mixtures
US20020110493A1 (en) * 1998-08-13 2002-08-15 Symyx Technologies , Inc Apparatus and methods for parallel processing of multiple reaction mixtures
US6864091B1 (en) * 2000-08-31 2005-03-08 Symyx Technologies, Inc. Sampling probe
US20090110606A1 (en) * 2006-06-21 2009-04-30 Olympus Corporation Dispensing apparatus and analyzer
US20110253750A1 (en) * 2010-04-20 2011-10-20 Koganei Corporation Liquid supply device
US8372340B2 (en) 2005-10-19 2013-02-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8476078B2 (en) 2005-10-19 2013-07-02 Luminex Corporation Cassette for sample preparation
US8900877B2 (en) 2006-12-27 2014-12-02 Luminex Corporation Instrument for cassette for sample preparation
AU2014201790B2 (en) * 2005-10-19 2015-12-17 Luminex Corporation Cassette for Sample Preparation
US9248422B2 (en) 2010-02-23 2016-02-02 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
AU2016201550B2 (en) * 2005-10-19 2017-07-27 Luminex Corporation Cassette for sample preparation
US11293416B2 (en) * 2017-09-21 2022-04-05 Hartmann's, Inc. Precision fluid dispensing device

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DE2910946A1 (en) * 1979-03-20 1980-10-02 Siemens Ag DOSING SYRINGE
FR2513376A1 (en) * 1981-09-23 1983-03-25 Jean Bajard AUTOMATIC PIPETTING DEVICE
DE10201587A1 (en) * 2002-01-17 2003-08-14 Renk Ag Elastic bolt coupling with two coupling halves joined by at least two bolts comprises coupling sleeves (6) whose inner element (13) consists of polyamide

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US1896134A (en) * 1929-02-25 1933-02-07 Dolph M Clark Liquid dispensing apparatus
US2216890A (en) * 1939-12-01 1940-10-08 Dalkin & Co A Measuring and mixing valve
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3421858A (en) * 1965-12-07 1969-01-14 Hewlett Packard Co Sampling apparatus
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US1896134A (en) * 1929-02-25 1933-02-07 Dolph M Clark Liquid dispensing apparatus
US2216890A (en) * 1939-12-01 1940-10-08 Dalkin & Co A Measuring and mixing valve
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3475130A (en) * 1965-07-08 1969-10-28 Warner Lambert Pharmaceutical Sample transfer apparatus
US3421858A (en) * 1965-12-07 1969-01-14 Hewlett Packard Co Sampling apparatus

Cited By (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3699348A (en) * 1970-08-14 1972-10-17 Zeiss Stiftung Radiation sensitive device for the successive investigation of several samples
US3739821A (en) * 1971-04-26 1973-06-19 Technicon Instr Machine-transferrable pipette
US3827304A (en) * 1971-07-20 1974-08-06 Gilson W Sample handling method
US3735902A (en) * 1971-07-22 1973-05-29 Instrumentation Labor Inc Dispenser apparatus
US3767364A (en) * 1971-07-30 1973-10-23 Sherwood Medical Ind Inc Reagent pipette system
DE2622691A1 (en) * 1976-05-21 1977-12-01 Technicon Instr Sample analyser with suction probe - can be immersed in liq. to suck it in, withdrawn and rinsed in top position before immersion in next sample
DE3015051A1 (en) * 1979-04-19 1980-10-23 Olympus Optical Co METHOD FOR DELIVERING SAMPLES AND REAGENTS IN REACTION TUBES
DE3033680A1 (en) * 1979-09-10 1981-03-12 Olympus Optical Co., Ltd., Tokyo DOSING AND LINE SYSTEM FOR ANALYZER DEVICES
US4318885A (en) * 1979-09-10 1982-03-09 Olympus Optical Co., Ltd. Liquid treating device for chemical analysis apparatus
US4318884A (en) * 1979-12-07 1982-03-09 Olympus Optical Co., Ltd. Distributing nozzle with cooperating ionic detector
US4399711A (en) * 1980-04-18 1983-08-23 Beckman Instruments, Inc. Method and apparatus ensuring full volume pickup in an automated pipette
US4340153A (en) * 1980-11-28 1982-07-20 Spivey David L Method and apparatus for medication dispensing
US4727033A (en) * 1981-12-17 1988-02-23 Olympus Optical Co., Ltd. Analyzing apparatus and method for immunological agglutination reactions
WO1990008307A1 (en) * 1988-12-29 1990-07-26 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5201232A (en) * 1988-12-29 1993-04-13 Technicon Instruments Corporation Integrated sampler for closed and open sample containers
US5158748A (en) * 1990-01-18 1992-10-27 Mochida Pharmaceutical Co., Ltd. Automated dispensing and diluting system
US5841039A (en) * 1991-04-04 1998-11-24 Technicon Instruments Corp. Apparatus and method for integrated sampling from closed and open sample containers
US5364240A (en) * 1993-10-14 1994-11-15 Spartan Tool Div. Of Pettibone Corp. Fluid pump with pulsing feature
US5795784A (en) * 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) * 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US6562298B1 (en) 1996-09-19 2003-05-13 Abbott Laboratories Structure for determination of item of interest in a sample
US6913934B2 (en) 1998-08-13 2005-07-05 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US20020110493A1 (en) * 1998-08-13 2002-08-15 Symyx Technologies , Inc Apparatus and methods for parallel processing of multiple reaction mixtures
US6302168B1 (en) * 2000-07-07 2001-10-16 Hugo Hu Automated precision liquid metering apparatus using injectors as metering devices
US6864091B1 (en) * 2000-08-31 2005-03-08 Symyx Technologies, Inc. Sampling probe
US7071000B2 (en) 2000-08-31 2006-07-04 Symyx Technologies, Inc. Method for sampling reaction products
US20040241875A1 (en) * 2001-01-26 2004-12-02 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US6759014B2 (en) 2001-01-26 2004-07-06 Symyx Technologies, Inc. Apparatus and methods for parallel processing of multiple reaction mixtures
US20010034067A1 (en) * 2001-01-26 2001-10-25 Dales G. Cameron Apparatus and methods for parallel processing of multiple reaction mixtures
AU2014201790B2 (en) * 2005-10-19 2015-12-17 Luminex Corporation Cassette for Sample Preparation
US10646875B2 (en) 2005-10-19 2020-05-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8372340B2 (en) 2005-10-19 2013-02-12 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
US8476078B2 (en) 2005-10-19 2013-07-02 Luminex Corporation Cassette for sample preparation
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Also Published As

Publication number Publication date
FR2000695A1 (en) 1969-09-12
JPS4947477B1 (en) 1974-12-16
DE1902297A1 (en) 1969-09-11
NL6900596A (en) 1969-07-29
SE327840B (en) 1970-08-31
DE1902297B2 (en) 1971-05-19
GB1256746A (en) 1971-12-15

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