US3663175A - Method of determining hemoglobin in blood - Google Patents
Method of determining hemoglobin in blood Download PDFInfo
- Publication number
- US3663175A US3663175A US78159A US3663175DA US3663175A US 3663175 A US3663175 A US 3663175A US 78159 A US78159 A US 78159A US 3663175D A US3663175D A US 3663175DA US 3663175 A US3663175 A US 3663175A
- Authority
- US
- United States
- Prior art keywords
- hemoglobin
- reagent
- moles
- acetone
- cyanmeth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (4)
- 2. The method according to claim 1 wherein the test solution contains about 61 X 10 5 moles of potassium ferricyanide, 77 X 10 5 moles of potassium cyanide and 1190 X 10 5 moles of sodium bicarbonate per liter.
- 3. The method of inhibiting loss of color in a hemoglobin test solution containing about 61 X 10 5 moles of potassium ferricyanide, 77 X 10 5 moles of potassium cyanide and 1,190 X 10 5 moles of sodium bicarbonate per liter, on exposure of said solution to temperatures below 32* F which comprises adding 0.2 to 10 weight percent acetone to said solution.
- 4. In the determination of the hemoglobin content of blood using a solution containing potassium cyanide, potassium ferricyanide and sodium bicarbonate in which the potassium ferricyanide is reduced to potassium ferrocyanide the improvement comprising adding from 0.2 to 10 weight percent acetone to said solution to assure immediate attainment of the peak absorbance of cyanmeth-hemoglobin in the spectrophotometric determination of the hemoglobin of blood sample.
- 5. The method of immediately attaining the cyanmeth hemoglobin peak in the spectrophotometric determination of hemoglobin using the cyanmeth-hemoglobin procedure which comprises adding from about 2 1/2 to 126 ml of acetone per liter to the reagent used in making the determination.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7815970A | 1970-10-05 | 1970-10-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
US3663175A true US3663175A (en) | 1972-05-16 |
Family
ID=22142298
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US78159A Expired - Lifetime US3663175A (en) | 1970-10-05 | 1970-10-05 | Method of determining hemoglobin in blood |
Country Status (5)
Country | Link |
---|---|
US (1) | US3663175A (en) |
JP (1) | JPS5130796B1 (en) |
CA (1) | CA952799A (en) |
DE (1) | DE2149763C3 (en) |
GB (1) | GB1301635A (en) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3874852A (en) * | 1974-07-05 | 1975-04-01 | Coulter Diagnostics Inc | Reagent and method for determining leukocytes and hemoglobin in the blood |
US3950133A (en) * | 1971-10-20 | 1976-04-13 | Mallinckrodt, Inc. | Reagent formulations for assaying biological specimens and methods of preparing and using same |
US4077772A (en) * | 1977-05-31 | 1978-03-07 | Baxter Travenol Laboratories, Inc. | Method for the determination of hemoglobin in trace amounts |
US4349351A (en) * | 1980-07-01 | 1982-09-14 | Bayer Aktiengesellschaft | Reagent for the determination of haemoglobin |
US4526869A (en) * | 1980-09-24 | 1985-07-02 | Regents Of The University Of Minnesota | Method for quantitatively determining the concentration of hemoglobin in a biological sample |
US4543336A (en) * | 1982-02-12 | 1985-09-24 | Dragerwerk Aktiengesellschaft | Indicator for determining sulfur dioxide |
US4725555A (en) * | 1986-10-22 | 1988-02-16 | Helena Laboratories Corporation | Zinc protoporphyrin test system |
US5426032A (en) * | 1986-08-13 | 1995-06-20 | Lifescan, Inc. | No-wipe whole blood glucose test strip |
US6458326B1 (en) | 1999-11-24 | 2002-10-01 | Home Diagnostics, Inc. | Protective test strip platform |
US6525330B2 (en) | 2001-02-28 | 2003-02-25 | Home Diagnostics, Inc. | Method of strip insertion detection |
US6541266B2 (en) | 2001-02-28 | 2003-04-01 | Home Diagnostics, Inc. | Method for determining concentration of an analyte in a test strip |
US6562625B2 (en) | 2001-02-28 | 2003-05-13 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52141392A (en) * | 1976-05-21 | 1977-11-25 | Mitsui Miike Machinery Co Ltd | Process and apparatus for intermittent throwing of baits for fish culture |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2940448A (en) * | 1957-04-29 | 1960-06-14 | Jr Norman B Furlong | Disposable blood-gas analyzer |
US3427135A (en) * | 1966-07-11 | 1969-02-11 | Technicon Instr | Hematology apparatus |
US3519572A (en) * | 1966-10-03 | 1970-07-07 | Pfizer & Co C | Blood control |
US3546131A (en) * | 1968-07-05 | 1970-12-08 | Uni Tech Chem Mfg Co | Stabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone |
-
1970
- 1970-10-05 US US78159A patent/US3663175A/en not_active Expired - Lifetime
-
1971
- 1971-09-16 CA CA123,049A patent/CA952799A/en not_active Expired
- 1971-09-17 GB GB4352071A patent/GB1301635A/en not_active Expired
- 1971-10-05 JP JP46078238A patent/JPS5130796B1/ja active Pending
- 1971-10-05 DE DE2149763A patent/DE2149763C3/en not_active Expired
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2940448A (en) * | 1957-04-29 | 1960-06-14 | Jr Norman B Furlong | Disposable blood-gas analyzer |
US3427135A (en) * | 1966-07-11 | 1969-02-11 | Technicon Instr | Hematology apparatus |
US3519572A (en) * | 1966-10-03 | 1970-07-07 | Pfizer & Co C | Blood control |
US3546131A (en) * | 1968-07-05 | 1970-12-08 | Uni Tech Chem Mfg Co | Stabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone |
Cited By (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3950133A (en) * | 1971-10-20 | 1976-04-13 | Mallinckrodt, Inc. | Reagent formulations for assaying biological specimens and methods of preparing and using same |
US3874852A (en) * | 1974-07-05 | 1975-04-01 | Coulter Diagnostics Inc | Reagent and method for determining leukocytes and hemoglobin in the blood |
US4077772A (en) * | 1977-05-31 | 1978-03-07 | Baxter Travenol Laboratories, Inc. | Method for the determination of hemoglobin in trace amounts |
US4349351A (en) * | 1980-07-01 | 1982-09-14 | Bayer Aktiengesellschaft | Reagent for the determination of haemoglobin |
US4526869A (en) * | 1980-09-24 | 1985-07-02 | Regents Of The University Of Minnesota | Method for quantitatively determining the concentration of hemoglobin in a biological sample |
US4543336A (en) * | 1982-02-12 | 1985-09-24 | Dragerwerk Aktiengesellschaft | Indicator for determining sulfur dioxide |
US6268162B1 (en) | 1986-08-13 | 2001-07-31 | Lifescan, Inc. | Reflectance measurement of analyte concentration with automatic initiation of timing |
US6881550B2 (en) | 1986-08-13 | 2005-04-19 | Roger Phillips | Method for the determination of glucose employing an apparatus emplaced matrix |
US5563042A (en) * | 1986-08-13 | 1996-10-08 | Lifescan, Inc. | Whole blood glucose test strip |
US5843692A (en) * | 1986-08-13 | 1998-12-01 | Lifescan, Inc. | Automatic initiation of a time interval for measuring glucose concentration in a sample of whole blood |
US6887426B2 (en) | 1986-08-13 | 2005-05-03 | Roger Phillips | Reagents test strip adapted for receiving an unmeasured sample while in use in an apparatus |
US5426032A (en) * | 1986-08-13 | 1995-06-20 | Lifescan, Inc. | No-wipe whole blood glucose test strip |
US6858401B2 (en) | 1986-08-13 | 2005-02-22 | Lifescan, Inc. | Minimum procedure system for the determination of analytes |
US6821483B2 (en) | 1986-08-13 | 2004-11-23 | Lifescan, Inc. | Reagents test strip with alignment notch |
US20030073151A1 (en) * | 1986-08-13 | 2003-04-17 | Roger Phillips | Minimum procedure system |
US20030073153A1 (en) * | 1986-08-13 | 2003-04-17 | Roger Phillips | Minimum procedure system for the determination of analytes |
US4725555A (en) * | 1986-10-22 | 1988-02-16 | Helena Laboratories Corporation | Zinc protoporphyrin test system |
US6458326B1 (en) | 1999-11-24 | 2002-10-01 | Home Diagnostics, Inc. | Protective test strip platform |
US6979571B2 (en) | 1999-11-24 | 2005-12-27 | Home Diagnostics, Inc. | Method of using a protective test strip platform for optical meter apparatus |
US6562625B2 (en) | 2001-02-28 | 2003-05-13 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
US6541266B2 (en) | 2001-02-28 | 2003-04-01 | Home Diagnostics, Inc. | Method for determining concentration of an analyte in a test strip |
US6525330B2 (en) | 2001-02-28 | 2003-02-25 | Home Diagnostics, Inc. | Method of strip insertion detection |
US7390665B2 (en) | 2001-02-28 | 2008-06-24 | Gilmour Steven B | Distinguishing test types through spectral analysis |
Also Published As
Publication number | Publication date |
---|---|
CA952799A (en) | 1974-08-13 |
DE2149763C3 (en) | 1973-09-13 |
DE2149763A1 (en) | 1972-04-06 |
JPS5130796B1 (en) | 1976-09-02 |
DE2149763B2 (en) | 1973-03-01 |
GB1301635A (en) | 1973-01-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: BOEHRINGER MANNHEIM DIAGNOSTICS, INC., 9115 HAGUE Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:BIO-DYNAMICS, INC.;REEL/FRAME:004239/0504 Effective date: 19831028 |
|
AS | Assignment |
Owner name: BOEHRINGER MANNHEIM CORPORATION, A CORP. OF NEW YO Free format text: CHANGE OF NAME;ASSIGNOR:BOEHRINGER MANNHEIM DIAGNOSTICS, INC. A CORP. OF DE.;REEL/FRAME:004624/0055 Effective date: 19861008 |
|
AS | Assignment |
Owner name: BOEHRINGER MANNHEIM CORPORATION Free format text: MERGER;ASSIGNOR:BOEHRINGER MANNHEIM CORPORATION, A NY CORP.;REEL/FRAME:004912/0770 Effective date: 19870625 Owner name: BOEHRINGER MANNHEIM CORPORATION, A IN CORP. Free format text: MERGER;ASSIGNOR:BOEHRINGER MANNHEIM CORPORATION, A NY CORP.;REEL/FRAME:004912/0770 Effective date: 19870625 |