US3751341A - Receptacle having a distendable sidewall - Google Patents
Receptacle having a distendable sidewall Download PDFInfo
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- US3751341A US3751341A US00146730A US3751341DA US3751341A US 3751341 A US3751341 A US 3751341A US 00146730 A US00146730 A US 00146730A US 3751341D A US3751341D A US 3751341DA US 3751341 A US3751341 A US 3751341A
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- receptacle
- sidewall
- cover
- distendable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/06—Means for testing the completeness of the sterilization
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Definitions
- the procedure generally accepted as being the most accurate is normally referred to as the swab-dilution-plating technique.
- Materials required for this procedure include sterile cotton-tipped applicators packaged in test tubes or other sterile individual containers, plastic templates, each about 4 sq. in. and aseptically packaged, dilution blanks each containing a few ml. of a suitable sterile non-toxic diluent in sterile test tubes, serologic pipettes, each of about one ml. capacity, and Petri dishes, each containing a suitable sterile agar medium.
- the test is performed by placing aseptically the template over the area to be tested; dipping aseptically a cotton-tipped applicator into a dilution blank of diluent; removing the applicator and swabbing the 4 sq. in. area within the confines of the template; placing the swab back into the dilution blank and returning the same to the laboratory where the blank is diluted serially in 10-fold dilutions to 1l00,000 or higher final dilutions.
- One ml. aliquots of the dilutions from 0 to the highest final dilution are pipetted aseptically to respective sterile, empty, marked Petri dishes. Then 10 ml.
- the Forgacs device requires a flexible fabric insert with a tab which necessitates a secondary assembly step, thereby resulting in a higher cost than that which is most desired.
- a gelled culture medium is retained on the insert in the Forgacs device.
- the device requires removal of the insert with the gel from the container for contacting the surface to be tested, followed by its replacement in the container for incubation.
- Still another variation of the surface contact type of device employs a flexible bottom whereby slight depression of the bottom forces the gel plug outward toward the test surface to be contacted.
- Said device has the disadvantage in that only the center portion of the gel surface tends to make contact with the test surface and, occasionally, the entire gel plug is inadvertently pushed out of the container.
- the device of the present invention which is an improvement over the foregoing surface contact devices, takes the general shape of a Petri dish, being cylindrical with an upwardly extending peripheral wall.
- the dish is adapted to contain a gelled culture medium such as agar.
- the upwardly extending wall terminates in a radially directed upper portion which preferably is thinner as it reaches the end.
- the radially directed portion therefore, presents an annular rim.
- the material of the dish is plastic so that it may be easily fabricated into the descending thickness. As a result of the thinning wall and flexible nature of the plastic, the wall can be distended radially when the open portion of the dish is pressed against a flat surface to be tested.
- the upper surface of the gel in the dish is brought to confrontation with the mentioned fiat surface as a result of the distension so that microorganisms on the flat surface may be picked up by the surface of the agar.
- Suitable removable covering means is provided to protect the agar surface both before and after use of the dish in contacting the test surface.
- FIG. 1 is a perspective view of an embodiment of the receptacle of the present invention suitably packaged.
- FIG. 2 is a top plan view with part of the cover broken away.
- FIG. 3 is a cross-sectional view taken along line 33 of FIG. 2.
- FIG. 4 depicts use of the invention.
- FIG. 5 is an enlarged cross-sectional view of a portion of another embodiment of the present invention.
- reference numeral 11 refers, generally, to the receptable of the present invention.
- the receptacle 11 with a cover 12 in place is enclosed in a protective plastic bag 13.
- the bag is preferably sterilized and sealed to prevent the admission of air, moisture, dirt and other contaminating materials.
- the receptacle contains a gelled culture medium 14 such as hardened agar.
- the receptacle consists of two parts, a lower part 15 and a cover 12.
- the lower part 15 has a shallow (e.g., 4-1 inch depth) cylindrical configuration with an upwardly extending sidewall 16 and a disc shaped bottom 17.
- the sidewall has an arcuate portion 18 which can comprise a rolled rim. The wall becomes thinner in the direction of its leading edge.
- the lower part 15 is constructed of plastic in a manner so that the.
- sidewall 16 is flexible at its upper portion and increases in flexibility as it becomes thinner.
- Suitable plastics for fabricating the receptacle are, for example, polystyrene, polyethylene, polypropylene, polyvinylchloride, cellulose acetate and methacryate polymers.
- the receptacle can be readily thermoformed or injection molded from these plastics to a degree of flexibility sufficient to permit distension of the upper portion of the sidewall when manually pressed against the surface to be tested, whereby the upper surface of the gel is brought into confrontation with said test surface.
- the arcuate portion 18 presents an annular appearance from the top.
- the arcuate portion has a plurality of apertures 21 spaced equidistant thereabout.
- the apertures will become distorted when the arcuate portion 18 of the sidewall 16 is distended and can serve to relieve hoop stress of the rolled rim.
- the apertures are useful to receive downwardly depending lugs on the cover 12. In this way the thin cover 12 is less likely to slip off the lower part 15.
- the edge of cover 12 has an annular arcuate edge portion adapted to be complementary with the arcuate portion 18 of the sidewall 16.
- FIG. depicts another embodiment in cover 19 structure.
- the cover 19 has a diameter greater than the largest diameter of the lower part 15. It also possesses a downwardly depending skirt 20.
- the cover may be of the type normally employed in covering a Petri dish so that from a manufacturing point of view only the lower part requires new fabricating facilities.
- a quantity of molten sterile agar or other gelling medium is poured into the lower part 15, much in the same manner as in connection with handling Petri dishes.
- the upper surface of the agar in the lower part preferably is at a level such that the agar volume is equivalent to about one-third to one-half of the capacity of the lower part 15.
- a cover is then placed over or on the lower part.
- the assembled receptacle is then packaged in a transparent envelope such as a polyethylene bag, for instance.
- a transparent envelope such as a polyethylene bag, for instance.
- the use of the bag may not be necessary when a large cover 19 is used so that the receptacle of the present invention may be handled as covered Petri dishes.
- the cover is removed and the lower part is positioned against a flat surface such as a wall 22, as in FIG. 4. It will be seen there from that the open portion of lower part is facing the wall 22.
- the sidewall 16 By pressing at the bottom 17 of the lower part 15, the sidewall 16 is distended along the arcuate portion 18 thereof so that the surface 23 of the agar makes contact with the wall, thereby picking up any microorganisms that may be present on the -wall.
- the lower part assumes its normal configuration.
- the cover is then repositioned and the receptacle is incubated employing conventional procedures therefor.
- the microorganism growth can be calculated from standards already known.
- a cover may in fact not be needed as long as the bag has sufiicient self-sustaining properties so that a portion thereof does not fall to the surface of the agar in the lower part of the receptacle.
- a plurality of upwardly extending and distendable finger-like projections or tabs can be positioned on the rim of the sidewall. These tabs can be equidistantly spaced apart in the manner shown for apertures 21 in FIG. 2.
- a removable plastic ring can be placed around the outer circumference of the lower part of the receptacle for supporting a thin, flexible side- -wall. The ring can be removed when contact with the test surface is desired to be made and then replaced for sup port prior to incubation of the receptacle.
- the sidewall can be notched, scored, or otherwise constructed to distend or break away under stress to allow the culture medium to contact the test surface.
- solid culture media for microorganisms other than agar can be employed in the receptacle of this invention, for instance, agarose, gelatin, carrageenin, modified cellulose such as described in US. Pat. 3,360,440, crosslinked polyacrylamide as disclosed in U.S. Pat. 3,046,021, irradiated polyethylene and the like natural and synthetic gelling substances.
- a test device for determining the presence of micro organisms on a relatively flat surface by direct contact therewith comprising a shallow cylindrical receptacle, said receptacle having a bottom and an upwardly extending cylindrical sidewall terminating in a leading edge, said receptacle including a quantity of gel medium the upper surface of which is substantially below said sidewall, said sidewall having a degree of flexibility to permit distension of its upper portion when manually pressed against said relatively flat surface whereby said upper surface of said medium is brought into contact with said relatively fiat surface due to the flexibility of the upper sidewall.
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Abstract
A ROUND RECEPTACLE IS DISCLOSED HAVING A DISTENDABLE SIDEWALL WHEREIN A GELLED CULTURE MEDIUM IS LOCATED IN THE BOTTOM OF THE RECEPTACLE. THE RECEPTACLE IS AN IMPROVED STERILITY TESTER AND/OR MICROORGANISM DETECTOR FOR SURFACES, AND MORE PARTICULARLY FOR DIRECT CONTACT WITH SURFACES WHICH ARE RELATIVELY FLAT.
Description
Aug. 7, 1973 L. J. SEITZ ET AL 3,751,341
RECEPTABLE HAVING A DISTENDABLE SIDEWALL Filed May 25, 1971 FIG. I. FIGZ.
IN VENTORS ATTORNEY United States Patent 3,751,341 RECEPTACLE HAVING A DISTENDABLE SIDEWALL Lamont J. Seitz, Huntington Beach, and Robert W. Bruen, South Laguna, Calif., assignors to Baxter Laboratories,
Inc., Morton Grove, Ill.
Filed May 25, 1971, Ser. No. 146,730 Int. Cl. 012k 1/00 U.S. Cl. 195-139 Claims ABSTRACT OF THE DISCLOSURE BACKGROUND OF THE INVENTION A serious problem is encountered in hospitals and other institutions, in determining whether a particular surface is sterile or in some cases to determine the nature and count of microorganisms on said surface. This is often a problem with bed sheets, surgical masks, surfaces in an operating room, or surfaces on the body of a patient. In the past, the practice most widely used involved extensive preparation of materials, and the steps involved in performing a sterility test required considerable effort, time and manipulation. For instance, for performing a sterility test on a single, fiat, nonporous area, the procedure generally accepted as being the most accurate is normally referred to as the swab-dilution-plating technique. Materials required for this procedure include sterile cotton-tipped applicators packaged in test tubes or other sterile individual containers, plastic templates, each about 4 sq. in. and aseptically packaged, dilution blanks each containing a few ml. of a suitable sterile non-toxic diluent in sterile test tubes, serologic pipettes, each of about one ml. capacity, and Petri dishes, each containing a suitable sterile agar medium. The test is performed by placing aseptically the template over the area to be tested; dipping aseptically a cotton-tipped applicator into a dilution blank of diluent; removing the applicator and swabbing the 4 sq. in. area within the confines of the template; placing the swab back into the dilution blank and returning the same to the laboratory where the blank is diluted serially in 10-fold dilutions to 1l00,000 or higher final dilutions. One ml. aliquots of the dilutions from 0 to the highest final dilution are pipetted aseptically to respective sterile, empty, marked Petri dishes. Then 10 ml. of sterile agar in test tubes are melted, cooled and added to each Petri dish containing the one ml. of the respective dilution of the test sample. The contents are allowed to solidify and then incubated from 18 to 24 hours in an incubator at 37 C. After the incubation, the number and types of microorganisms are reported as the number and types of organisms per a given area, viz, organisms and types per square inch of suface tested. Obviously, this procedure involves a great deal of labor and equipment and it enhances the possibility of foreign contamination.
Considerable progress away from the above described techniques was made with the advent of thetype of sterility testing device disclosed in US. Pat. No. 3,337,416 to Forgacs. This device is essentially a surface contact plate.
3,751,341 Patented Aug. 7, 1973 However, the Forgacs device requires a flexible fabric insert with a tab which necessitates a secondary assembly step, thereby resulting in a higher cost than that which is most desired. A gelled culture medium is retained on the insert in the Forgacs device. In operation, the device requires removal of the insert with the gel from the container for contacting the surface to be tested, followed by its replacement in the container for incubation.
Another improved device for microorganism detecting is illustrated in US. Pat. No. 3,203,870 to Andelin. However, the Andelin device is difficult to pour since the dish must be completely filled with the gelled medium so that the gel surface projects above the level of the upstanding sidewalls.
Still another variation of the surface contact type of device employs a flexible bottom whereby slight depression of the bottom forces the gel plug outward toward the test surface to be contacted. Said device has the disadvantage in that only the center portion of the gel surface tends to make contact with the test surface and, occasionally, the entire gel plug is inadvertently pushed out of the container.
SUMMARY OF THE INVENTION The device of the present invention, which is an improvement over the foregoing surface contact devices, takes the general shape of a Petri dish, being cylindrical with an upwardly extending peripheral wall. The dish is adapted to contain a gelled culture medium such as agar. The upwardly extending wall terminates in a radially directed upper portion which preferably is thinner as it reaches the end. The radially directed portion, therefore, presents an annular rim. The material of the dish is plastic so that it may be easily fabricated into the descending thickness. As a result of the thinning wall and flexible nature of the plastic, the wall can be distended radially when the open portion of the dish is pressed against a flat surface to be tested. The upper surface of the gel in the dish is brought to confrontation with the mentioned fiat surface as a result of the distension so that microorganisms on the flat surface may be picked up by the surface of the agar. Suitable removable covering means is provided to protect the agar surface both before and after use of the dish in contacting the test surface.
DETAILED DESCRIPTION OF THE INVENTION 'In the following, like reference numerals refer to like parts throughout the drawings wherein:
FIG. 1 is a perspective view of an embodiment of the receptacle of the present invention suitably packaged.
FIG. 2 is a top plan view with part of the cover broken away.
FIG. 3 is a cross-sectional view taken along line 33 of FIG. 2.
FIG. 4 depicts use of the invention.
FIG. 5 is an enlarged cross-sectional view of a portion of another embodiment of the present invention.
Turning to the drawings, reference numeral 11 refers, generally, to the receptable of the present invention. In FIG. 1 the receptacle 11 with a cover 12 in place is enclosed in a protective plastic bag 13. The bag is preferably sterilized and sealed to prevent the admission of air, moisture, dirt and other contaminating materials. The receptacle contains a gelled culture medium 14 such as hardened agar.
From FIG. 3 it can be seen that the receptacle consists of two parts, a lower part 15 and a cover 12. The lower part 15 has a shallow (e.g., 4-1 inch depth) cylindrical configuration with an upwardly extending sidewall 16 and a disc shaped bottom 17. The sidewall has an arcuate portion 18 which can comprise a rolled rim. The wall becomes thinner in the direction of its leading edge. The lower part 15 is constructed of plastic in a manner so that the.
sidewall 16 is flexible at its upper portion and increases in flexibility as it becomes thinner.
Suitable plastics for fabricating the receptacle are, for example, polystyrene, polyethylene, polypropylene, polyvinylchloride, cellulose acetate and methacryate polymers. The receptacle can be readily thermoformed or injection molded from these plastics to a degree of flexibility sufficient to permit distension of the upper portion of the sidewall when manually pressed against the surface to be tested, whereby the upper surface of the gel is brought into confrontation with said test surface.
As can be seen from FIG. 2, the arcuate portion 18 presents an annular appearance from the top. The arcuate portion has a plurality of apertures 21 spaced equidistant thereabout. The apertures will become distorted when the arcuate portion 18 of the sidewall 16 is distended and can serve to relieve hoop stress of the rolled rim. Also, the apertures are useful to receive downwardly depending lugs on the cover 12. In this way the thin cover 12 is less likely to slip off the lower part 15. The edge of cover 12 has an annular arcuate edge portion adapted to be complementary with the arcuate portion 18 of the sidewall 16.
FIG. depicts another embodiment in cover 19 structure. The cover 19 has a diameter greater than the largest diameter of the lower part 15. It also possesses a downwardly depending skirt 20. The cover may be of the type normally employed in covering a Petri dish so that from a manufacturing point of view only the lower part requires new fabricating facilities.
Prior to use, a quantity of molten sterile agar or other gelling medium is poured into the lower part 15, much in the same manner as in connection with handling Petri dishes. The upper surface of the agar in the lower part preferably is at a level such that the agar volume is equivalent to about one-third to one-half of the capacity of the lower part 15.
A cover is then placed over or on the lower part. The assembled receptacle is then packaged in a transparent envelope such as a polyethylene bag, for instance. The use of the bag may not be necessary when a large cover 19 is used so that the receptacle of the present invention may be handled as covered Petri dishes. When the device is to be utilized, the cover is removed and the lower part is positioned against a flat surface such as a wall 22, as in FIG. 4. It will be seen there from that the open portion of lower part is facing the wall 22. By pressing at the bottom 17 of the lower part 15, the sidewall 16 is distended along the arcuate portion 18 thereof so that the surface 23 of the agar makes contact with the wall, thereby picking up any microorganisms that may be present on the -wall.When the pressure on the bottom is released, the lower part assumes its normal configuration. The cover is then repositioned and the receptacle is incubated employing conventional procedures therefor. The microorganism growth can be calculated from standards already known.
When a sterilized bag is employed, a cover may in fact not be needed as long as the bag has sufiicient self-sustaining properties so that a portion thereof does not fall to the surface of the agar in the lower part of the receptacle.
One of the advantages of the present invention merits mention at this point although others will be readily apparent. While the principal use of sterility or bacteriological identification with the present device is in a hospital, that is not the only use of the invention. Frequently a doctor calling on a patient at his home or in the case of office calls may wish to make tests of selected areas. These are more apt to be tests in which the identification of the microorganism is of importance, but occasionally they are also sterility tests. The physician can keep a supply of receptacles of the present invention in his office and take a few with him in his doctors bag and can make the tests and then send the receptacles to a suitable laboratory for examination, for example, at the nearest hospital. This convenience, even for the individual physician, is of importance and, of course, the enhanced reliability of the tests is just as important whether the test is made by an individual physican or is made in conjunction with a surgical team of a hospital.
Although particular reference has been made hereinbefore to use of the claimed invention in hospitals and by physicians, it will be understood that the invention is not limited thereto but is similiarly applicable for use in food laboratories, dairies, meat packing houses and other such institutions having a need to determine sterility and/or the nature and count of microorganisms.
While the invention has been described in detail with respect to particular embodiments in which the receptacle is distendable along the arcuate and thinner portion of the sidewall, it will be appreciated that the objects of the invention can also be achieved by providing convolutions in said sidewall which will distend or yield under stress and allow the gelled medium to contact the surface as the receptacle is pressed against a fiat surface.
In another embodiment of the invention, a plurality of upwardly extending and distendable finger-like projections or tabs can be positioned on the rim of the sidewall. These tabs can be equidistantly spaced apart in the manner shown for apertures 21 in FIG. 2.
In still another embodiment, a removable plastic ring can be placed around the outer circumference of the lower part of the receptacle for supporting a thin, flexible side- -wall. The ring can be removed when contact with the test surface is desired to be made and then replaced for sup port prior to incubation of the receptacle.
In yet another embodiment, the sidewall can be notched, scored, or otherwise constructed to distend or break away under stress to allow the culture medium to contact the test surface.
It should also be noted that although agar has been particularly described to illustrate the gelled nutrient medium, solid culture media for microorganisms other than agar can be employed in the receptacle of this invention, for instance, agarose, gelatin, carrageenin, modified cellulose such as described in US. Pat. 3,360,440, crosslinked polyacrylamide as disclosed in U.S. Pat. 3,046,021, irradiated polyethylene and the like natural and synthetic gelling substances.
While there have been shown and described particular embodiments of the invention, it will be obvious to those skilled in the art that various changes and modifications can be made therein without departing from the invention and, therefore, it is aimed to cover all such changes and modifications as fall within the true spirit and scope of the invention.
We claim:
1. A test device for determining the presence of micro organisms on a relatively flat surface by direct contact therewith comprising a shallow cylindrical receptacle, said receptacle having a bottom and an upwardly extending cylindrical sidewall terminating in a leading edge, said receptacle including a quantity of gel medium the upper surface of which is substantially below said sidewall, said sidewall having a degree of flexibility to permit distension of its upper portion when manually pressed against said relatively flat surface whereby said upper surface of said medium is brought into contact with said relatively fiat surface due to the flexibility of the upper sidewall.
2. The device of claim 1 wherein said sidewall has an arcuate radially extending portion at the upper portion thereof.
3. The device of claim 2 wherein the receptacle is distendable along said arcuate portion.
4. The device of claim 2 wherein said sidewall tapers in thickness from at least the beginning portion of the arcuate portion towards the said leading edge.
5. The device of claim 4 wherein at least the arcuate portion of the sidewall is constructed of plastic.
6. The device of claim 5 wherein said arcuate portion has a plurality of apertures.
7. The device of claim 6 wherein the receptacle has a cover.
8. The device of claim 7 wherein the cover has a plurality of depending lugs adapted to fit into said apertures.
9. The device of claim 4 wherein the arcuate portion is resilient.
10. The device of claim 1 wherein said sidewall is convoluted.
References Cited UNITED STATES PATENTS 3,386,793 6/1968 Stanton 401-132 2,852,054 9/1958 Motley l50-O.5 3,203,870 8/1965 Andelin 195139 3,198,713 8/1965 McCormick 195139 10 LIONEL M. SHAPIRO, Primary Examiner R. B. PENLAND, Assistant Examiner
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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US14673071A | 1971-05-25 | 1971-05-25 |
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US3751341A true US3751341A (en) | 1973-08-07 |
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Application Number | Title | Priority Date | Filing Date |
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US00146730A Expired - Lifetime US3751341A (en) | 1971-05-25 | 1971-05-25 | Receptacle having a distendable sidewall |
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CA (1) | CA975270A (en) |
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Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4071320A (en) * | 1976-04-27 | 1978-01-31 | Martin Korbin | Device for the quantitative determination of impurities, especially bacteria, on flat surfaces |
US4160700A (en) * | 1977-02-14 | 1979-07-10 | Gelman Instrument Company | Petri dish |
EP0013748A1 (en) * | 1979-01-30 | 1980-08-06 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Unit for culture sampling by contact |
US4237223A (en) * | 1978-04-21 | 1980-12-02 | Merck Patent Gesellschaft Mit Beschrankter Haftung | Sheet for picking off microorganisms |
WO1982002563A1 (en) * | 1981-01-27 | 1982-08-05 | Minnesota Mining & Mfg | Dry culture media |
EP0085839A2 (en) * | 1982-02-05 | 1983-08-17 | FMC Corporation | Colony replicating device |
US4565783A (en) * | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
US4598050A (en) * | 1983-12-02 | 1986-07-01 | Brown Lewis R | Culture plate for surfaces |
US4608342A (en) * | 1983-05-13 | 1986-08-26 | Stephan Nees | Method of growing a confluent layer of cells on a porous or semi-permeable substrate and apparatus for practicing the method |
US4709819A (en) * | 1986-07-23 | 1987-12-01 | Environmental Diagnostics, Inc. | Method for preserving plated media and product |
US4717667A (en) * | 1984-07-11 | 1988-01-05 | Fmc Corporation | Colony replicating device |
US5593891A (en) * | 1994-11-10 | 1997-01-14 | Banes; Albert J. | Culture plate with splash guard |
US5695988A (en) * | 1992-05-22 | 1997-12-09 | Chong; Sue Kheng | Culture device for sampling and/or counting micro-organism |
US6670174B1 (en) * | 1990-03-12 | 2003-12-30 | Difco Laboratories Incorporated | Culture dish package and method of making |
ES2204326A1 (en) * | 2002-10-03 | 2004-04-16 | Henkel Iberica S.A. | Procedure for determining microbial contamination of hard surface involves joining separably sterile plate to surface and removal of plate after specific period followed by incubation of plate with culture medium |
US20120261277A1 (en) * | 2011-04-18 | 2012-10-18 | Nanologix, Inc. | Flat Packaging of Petri Dishes for Prolonged Preservation and Method of Producing the Same |
USD755625S1 (en) | 2012-09-10 | 2016-05-10 | The Hillshire Brands Company | Packaging for food product |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2116709B (en) * | 1982-03-09 | 1986-02-19 | Wira & Mather | Microbiological testing detecting surface mildew |
GB2157708B (en) * | 1984-01-24 | 1988-03-30 | Eric James Sjoberg | Collecting and preserving microorganisms |
GB2330908A (en) * | 1997-10-30 | 1999-05-05 | Guy James Rollo Symons | Sampling a surface for a contaminant |
EP2261316A1 (en) | 2009-06-12 | 2010-12-15 | Nutricion Infantil Funcional y Segura A.I.E. | Method and device for determining the microbiological contamination in an environment |
-
1971
- 1971-05-25 US US00146730A patent/US3751341A/en not_active Expired - Lifetime
-
1972
- 1972-05-23 GB GB2415872A patent/GB1379506A/en not_active Expired
- 1972-05-24 CA CA142,860A patent/CA975270A/en not_active Expired
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4071320A (en) * | 1976-04-27 | 1978-01-31 | Martin Korbin | Device for the quantitative determination of impurities, especially bacteria, on flat surfaces |
US4160700A (en) * | 1977-02-14 | 1979-07-10 | Gelman Instrument Company | Petri dish |
US4237223A (en) * | 1978-04-21 | 1980-12-02 | Merck Patent Gesellschaft Mit Beschrankter Haftung | Sheet for picking off microorganisms |
EP0013748A1 (en) * | 1979-01-30 | 1980-08-06 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Unit for culture sampling by contact |
US4565783A (en) * | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
WO1982002563A1 (en) * | 1981-01-27 | 1982-08-05 | Minnesota Mining & Mfg | Dry culture media |
US4659672A (en) * | 1982-02-05 | 1987-04-21 | Fmc Corporation | Colony replicating device |
EP0085839A2 (en) * | 1982-02-05 | 1983-08-17 | FMC Corporation | Colony replicating device |
EP0085839A3 (en) * | 1982-02-05 | 1984-12-05 | FMC Corporation | Colony replicating device |
US4608342A (en) * | 1983-05-13 | 1986-08-26 | Stephan Nees | Method of growing a confluent layer of cells on a porous or semi-permeable substrate and apparatus for practicing the method |
US4598050A (en) * | 1983-12-02 | 1986-07-01 | Brown Lewis R | Culture plate for surfaces |
US4717667A (en) * | 1984-07-11 | 1988-01-05 | Fmc Corporation | Colony replicating device |
US4709819A (en) * | 1986-07-23 | 1987-12-01 | Environmental Diagnostics, Inc. | Method for preserving plated media and product |
US6670174B1 (en) * | 1990-03-12 | 2003-12-30 | Difco Laboratories Incorporated | Culture dish package and method of making |
US5695988A (en) * | 1992-05-22 | 1997-12-09 | Chong; Sue Kheng | Culture device for sampling and/or counting micro-organism |
US5593891A (en) * | 1994-11-10 | 1997-01-14 | Banes; Albert J. | Culture plate with splash guard |
ES2204326A1 (en) * | 2002-10-03 | 2004-04-16 | Henkel Iberica S.A. | Procedure for determining microbial contamination of hard surface involves joining separably sterile plate to surface and removal of plate after specific period followed by incubation of plate with culture medium |
US20120261277A1 (en) * | 2011-04-18 | 2012-10-18 | Nanologix, Inc. | Flat Packaging of Petri Dishes for Prolonged Preservation and Method of Producing the Same |
US8413800B2 (en) * | 2011-04-18 | 2013-04-09 | Bret T. Barnhizer | Flat packaging of petri dishes for prolonged preservation and method of producing the same |
USD755625S1 (en) | 2012-09-10 | 2016-05-10 | The Hillshire Brands Company | Packaging for food product |
Also Published As
Publication number | Publication date |
---|---|
GB1379506A (en) | 1975-01-02 |
CA975270A (en) | 1975-09-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: COOPER LABORATORES, INC., 3145 PORTER DRIVE, PALO Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:BAXTER TRAVENOL LABORATORIES, INC., A CORP. OF DE;REEL/FRAME:004067/0785 Effective date: 19820917 |
|
AS | Assignment |
Owner name: TECHNICON INSTRUMENTS CORPORATION, 511 BENEDICT AV Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:COOPER LABORATORIES, INC.,;REEL/FRAME:004926/0396 Effective date: 19880628 |