US4451569A - Stable enzyme composition - Google Patents
Stable enzyme composition Download PDFInfo
- Publication number
- US4451569A US4451569A US06/394,165 US39416582A US4451569A US 4451569 A US4451569 A US 4451569A US 39416582 A US39416582 A US 39416582A US 4451569 A US4451569 A US 4451569A
- Authority
- US
- United States
- Prior art keywords
- composition
- glutathione peroxidase
- sugar alcohols
- enzyme
- stabilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
2GSH+H.sub.2 O.sub.2 →GSSG+2H.sub.2 O (1)
TABLE 1 ______________________________________ Activity retention (%)*.sup.1 Addition 0 1 3 6 Stabilizer ratio*.sup.2 day month months months ______________________________________ Pre- Arabinose 1 92 92 90 87 sent Glucose 1 94 92 89 84 inven- Xylitol 1 95 92 90 85 tion Sorbitol 1 95 92 90 88 Sucrose 1 97 95 95 93 Com- Erythrose 1 69 65 60 52 para- Heptulose 1 63 60 55 48 tive Erythritol 1 60 51 48 40 exam- Sedoheptitol 1 65 58 52 39 ple Amylose 1 70 65 50 35 No addition -- 50 30 27 20 ______________________________________ *.sup.1 Storage was carried out at 4° C. The activity retention wa calculated with the activity value before freeze drying as 100%. *.sup.2 Weight of added substance/weight of said enzyme protein.
TABLE 2 ______________________________________ Addition Activity retention (%) Stabilizer ratio 3 months 6 months ______________________________________ Arabinose 4 92 87 1 91 87 0.5 79 65 Glucose 4 90 85 1 90 84 0.5 77 63 Xylitol 4 91 86 1 89 85 0.5 78 66 Sorbitol 4 91 88 1 91 87 0.5 75 61 Sucrose 4 95 94 1 96 93 0.5 79 68 ______________________________________ Storage was carried out at 4° C.
TABLE 3 ______________________________________ Storage temperature Activity retention (%) Stabilizer (°C.) 3 months 6 months ______________________________________ Arabinose 30 71 61 20 90 84 4 91 86 Glucose 30 69 59 20 87 83 4 90 85 Xylitol 30 73 62 20 89 83 4 90 85 Sorbitol 30 72 60 20 90 86 4 91 88 Sucrose 30 71 60 20 95 92 4 96 94 ______________________________________
TABLE 4 ______________________________________ Activity retention (%) Stabilizer 0 day 1 month 3 months 6 months ______________________________________ None 100 65 40 35 Glutathione 100 70 60 49 Dithiothreitol 100 98 80 59 Glutathione + 100 98 90 60 dithiothreitol 24% Ethanol 100 98 80 40 solution ______________________________________
Claims (8)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56106130A JPS589688A (en) | 1981-07-06 | 1981-07-06 | Stable enzymic composition |
JP56-106130 | 1981-07-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
US4451569A true US4451569A (en) | 1984-05-29 |
Family
ID=14425818
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US06/394,165 Expired - Lifetime US4451569A (en) | 1981-07-06 | 1982-07-01 | Stable enzyme composition |
Country Status (5)
Country | Link |
---|---|
US (1) | US4451569A (en) |
JP (1) | JPS589688A (en) |
CH (1) | CH653703A5 (en) |
DE (1) | DE3225250A1 (en) |
SE (1) | SE8204148L (en) |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4684615A (en) * | 1983-06-06 | 1987-08-04 | Ciba Corning Diagnostics Corp. | Stabilized isoenzyme control products |
US4699882A (en) * | 1984-06-25 | 1987-10-13 | Suomen Sokeri Oi | Stable glucose isomerase concentrate and a process for the preparation thereof |
US4810633A (en) * | 1984-06-04 | 1989-03-07 | Miles Inc. | Enzymatic ethanol test |
US4992372A (en) * | 1989-02-02 | 1991-02-12 | Pokora Alexander R | Method for removing impurities from peroxidase solutions |
US5200462A (en) * | 1991-01-25 | 1993-04-06 | Eastman Kodak Company | Succinimide containing polymers and lattices prepared from same |
US5308749A (en) * | 1991-01-25 | 1994-05-03 | Eastman Kodak Company | Method of preparing biologically active reagents from succinimide-containing polymers, analytical element and methods of use |
WO1994028909A1 (en) * | 1993-06-11 | 1994-12-22 | Bicher Haim I | Antineoplastic compositions and methods of use |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
WO1996041870A1 (en) * | 1995-06-08 | 1996-12-27 | Societa' Cooperativa Centro Ricerche Poly-Tech A Responsabilita' Limitata | Hydrosoluble compositions of stabilized collagenase and the process for their preparation |
EP0778344A1 (en) * | 1994-07-20 | 1997-06-11 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing stabilized dry carboxyesterase |
WO1998000530A1 (en) * | 1996-07-03 | 1998-01-08 | Molecular Biology Resources, Inc. | Method and formulation for stabilization of enzymes |
US6509146B1 (en) | 1996-05-29 | 2003-01-21 | Universal Preservation Technologies, Inc. | Scalable long-term shelf preservation of sensitive biological solutions and suspensions |
US6667167B1 (en) * | 1997-03-12 | 2003-12-23 | Novozymes A/S | Storage-stable liquid formulation comprising a laccase |
US6835811B1 (en) * | 2001-03-13 | 2004-12-28 | Kromar Medical Corporation | Extended storage of reduced glutathione solutions |
US20050239214A1 (en) * | 2004-04-22 | 2005-10-27 | Medtox Scientific, Inc. | Non-sugar sweeteners for use in test devices |
US20050276728A1 (en) * | 2004-04-08 | 2005-12-15 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US20110081363A1 (en) * | 2009-05-11 | 2011-04-07 | Biomatrica, Inc. | Compositions and methods for biological sample storage |
US20140017318A1 (en) * | 2012-07-10 | 2014-01-16 | Kevin O'Connell | Method to produce a medicinal product comprising a biologically active protein and the resulting product |
US9314519B2 (en) | 2012-08-21 | 2016-04-19 | Intervet Inc. | Liquid stable virus vaccines |
US9376709B2 (en) | 2010-07-26 | 2016-06-28 | Biomatrica, Inc. | Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures |
US9393298B2 (en) | 2013-03-15 | 2016-07-19 | Intervet Inc. | Liquid stable bovine virus vaccines |
US9480739B2 (en) | 2013-03-15 | 2016-11-01 | Intervet Inc. | Bovine virus vaccines that are liquid stable |
US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
US9827305B2 (en) | 2014-02-17 | 2017-11-28 | Intervet Inc. | Poultry virus vaccines that are liquid stable |
US9839613B2 (en) | 2013-09-27 | 2017-12-12 | Intervet Inc. | Dry formulations of vaccines that are room temperature stable |
US9845489B2 (en) | 2010-07-26 | 2017-12-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
US9855336B2 (en) | 2014-02-19 | 2018-01-02 | Intervet Inc. | Swine virus vaccines that are liquid stable |
US10064404B2 (en) | 2014-06-10 | 2018-09-04 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
US10568317B2 (en) | 2015-12-08 | 2020-02-25 | Biomatrica, Inc. | Reduction of erythrocyte sedimentation rate |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1230300A (en) * | 1983-06-06 | 1987-12-15 | Michael K. Hoskins | Stabilized isoenzyme control product |
US4734360A (en) * | 1983-07-12 | 1988-03-29 | Lifescan, Inc. | Colorimetric ethanol analysis method and test device |
JPH0728741B2 (en) * | 1985-05-14 | 1995-04-05 | わかもと製薬株式会社 | Stable β-galactosidase aqueous composition |
DE3811659C1 (en) * | 1988-04-07 | 1989-11-16 | Henning Berlin Gmbh Chemie- Und Pharmawerk, 1000 Berlin, De | |
US5045446A (en) * | 1988-08-26 | 1991-09-03 | Cryopharm Corporation | Lyophilization of cells |
IL90188A0 (en) * | 1988-05-18 | 1989-12-15 | Cryopharm Corp | Process and medium for the lyophilization of erythrocytes |
JPH01304882A (en) * | 1988-06-03 | 1989-12-08 | Ube Ind Ltd | Stable storing method of superoxide dismutase of human cu, zn-type |
EP0356258A3 (en) * | 1988-08-26 | 1990-06-06 | Cryopharm Corporation | Processes for the lyophilization of red blood cells, together with media for lyophilization |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3133001A (en) * | 1959-11-26 | 1964-05-12 | Muset Pedro Puig | Stabilization of enzymes |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1517782A1 (en) * | 1966-05-05 | 1970-10-08 | Stafford Allen & Sons Ltd | Process for the production of a hop extract containing isohumolones |
NL7003678A (en) * | 1969-04-09 | 1970-10-13 | ||
CA958339A (en) * | 1970-09-19 | 1974-11-26 | Yoshio Yoshimura | Stabilization of microorganism cells lytic enzyme |
JPS4834912A (en) * | 1971-09-09 | 1973-05-23 | ||
DE2234412B2 (en) * | 1972-07-13 | 1980-07-03 | Henkel Kgaa, 4000 Duesseldorf | Process for the stabilization and precipitation of concentrated protease solutions |
JPS6053959B2 (en) * | 1978-07-06 | 1985-11-28 | 松下電器産業株式会社 | Electromagnetic pick-up cartridge |
-
1981
- 1981-07-06 JP JP56106130A patent/JPS589688A/en active Pending
-
1982
- 1982-07-01 US US06/394,165 patent/US4451569A/en not_active Expired - Lifetime
- 1982-07-05 CH CH4088/82A patent/CH653703A5/en not_active IP Right Cessation
- 1982-07-05 SE SE8204148A patent/SE8204148L/en not_active Application Discontinuation
- 1982-07-06 DE DE19823225250 patent/DE3225250A1/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3133001A (en) * | 1959-11-26 | 1964-05-12 | Muset Pedro Puig | Stabilization of enzymes |
Non-Patent Citations (4)
Title |
---|
Biochemistry vol. 13, pp. 1825 1828 (1974). * |
Biochemistry vol. 13, pp. 1825-1828 (1974). |
Nakamura et al., Biochimica et Biophysica Acta 358 (1974) 251 261. * |
Nakamura et al., Biochimica et Biophysica Acta 358 (1974) 251-261. |
Cited By (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4684615A (en) * | 1983-06-06 | 1987-08-04 | Ciba Corning Diagnostics Corp. | Stabilized isoenzyme control products |
US4810633A (en) * | 1984-06-04 | 1989-03-07 | Miles Inc. | Enzymatic ethanol test |
US4699882A (en) * | 1984-06-25 | 1987-10-13 | Suomen Sokeri Oi | Stable glucose isomerase concentrate and a process for the preparation thereof |
US4992372A (en) * | 1989-02-02 | 1991-02-12 | Pokora Alexander R | Method for removing impurities from peroxidase solutions |
US5200462A (en) * | 1991-01-25 | 1993-04-06 | Eastman Kodak Company | Succinimide containing polymers and lattices prepared from same |
US5308749A (en) * | 1991-01-25 | 1994-05-03 | Eastman Kodak Company | Method of preparing biologically active reagents from succinimide-containing polymers, analytical element and methods of use |
WO1994028909A1 (en) * | 1993-06-11 | 1994-12-22 | Bicher Haim I | Antineoplastic compositions and methods of use |
US5449663A (en) * | 1993-06-11 | 1995-09-12 | Bicher; Haim I. | Antineoplastic compositions |
EP0778344A1 (en) * | 1994-07-20 | 1997-06-11 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing stabilized dry carboxyesterase |
EP0778344A4 (en) * | 1994-07-20 | 1998-08-05 | Kyowa Hakko Kogyo Kk | Process for producing stabilized dry carboxyesterase |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
US5614387A (en) * | 1995-02-10 | 1997-03-25 | Gen-Probe Incorporated | Method of making stabilized enzyme compositions for nucleic acid amplification |
US5834254A (en) * | 1995-02-10 | 1998-11-10 | Gen-Probe Incorporated | Stabilized enzyme compositions for nucleic acid amplification |
WO1996041870A1 (en) * | 1995-06-08 | 1996-12-27 | Societa' Cooperativa Centro Ricerche Poly-Tech A Responsabilita' Limitata | Hydrosoluble compositions of stabilized collagenase and the process for their preparation |
US6509146B1 (en) | 1996-05-29 | 2003-01-21 | Universal Preservation Technologies, Inc. | Scalable long-term shelf preservation of sensitive biological solutions and suspensions |
WO1998000530A1 (en) * | 1996-07-03 | 1998-01-08 | Molecular Biology Resources, Inc. | Method and formulation for stabilization of enzymes |
US6294365B1 (en) | 1996-07-03 | 2001-09-25 | Molecular Biology Resources, Inc. | Method and formulation for stabilization of enzymes |
US5876992A (en) * | 1996-07-03 | 1999-03-02 | Molecular Biology Resources, Inc. | Method and formulation for stabilization of enzymes |
US6667167B1 (en) * | 1997-03-12 | 2003-12-23 | Novozymes A/S | Storage-stable liquid formulation comprising a laccase |
US6835811B1 (en) * | 2001-03-13 | 2004-12-28 | Kromar Medical Corporation | Extended storage of reduced glutathione solutions |
US20050130905A1 (en) * | 2001-03-13 | 2005-06-16 | Kromar Medical Corporation | Extended storage of reduced glutathione solutions |
US7449546B2 (en) | 2001-03-13 | 2008-11-11 | Kromar Medical Corporation | Method for extending storage of glutathione solutions and methods for treating Parkinson's disease with said stored glutathione solutions |
US9078426B2 (en) | 2004-04-08 | 2015-07-14 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US20050276728A1 (en) * | 2004-04-08 | 2005-12-15 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US8900856B2 (en) | 2004-04-08 | 2014-12-02 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
US20090291427A1 (en) * | 2004-04-08 | 2009-11-26 | Judy Muller-Cohn | Integration of sample storage and sample management for life science |
US7319032B2 (en) * | 2004-04-22 | 2008-01-15 | Medtox | Non-sugar sweeteners for use in test devices |
US20050239214A1 (en) * | 2004-04-22 | 2005-10-27 | Medtox Scientific, Inc. | Non-sugar sweeteners for use in test devices |
US8519125B2 (en) | 2009-05-11 | 2013-08-27 | Biomatrica, Inc. | Compositions and methods for biological sample storage |
US20110081363A1 (en) * | 2009-05-11 | 2011-04-07 | Biomatrica, Inc. | Compositions and methods for biological sample storage |
US9999217B2 (en) | 2010-07-26 | 2018-06-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA, and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
US9845489B2 (en) | 2010-07-26 | 2017-12-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
US9376709B2 (en) | 2010-07-26 | 2016-06-28 | Biomatrica, Inc. | Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures |
US20140017318A1 (en) * | 2012-07-10 | 2014-01-16 | Kevin O'Connell | Method to produce a medicinal product comprising a biologically active protein and the resulting product |
US9526780B2 (en) | 2012-08-21 | 2016-12-27 | Intervet Inc. | Liquid stable virus vaccines |
US9314519B2 (en) | 2012-08-21 | 2016-04-19 | Intervet Inc. | Liquid stable virus vaccines |
US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
US9393298B2 (en) | 2013-03-15 | 2016-07-19 | Intervet Inc. | Liquid stable bovine virus vaccines |
US9480739B2 (en) | 2013-03-15 | 2016-11-01 | Intervet Inc. | Bovine virus vaccines that are liquid stable |
US9603924B2 (en) | 2013-03-15 | 2017-03-28 | Intervet Inc. | Bovine virus vaccines that are liquid stable |
US9839613B2 (en) | 2013-09-27 | 2017-12-12 | Intervet Inc. | Dry formulations of vaccines that are room temperature stable |
US9827305B2 (en) | 2014-02-17 | 2017-11-28 | Intervet Inc. | Poultry virus vaccines that are liquid stable |
US9855336B2 (en) | 2014-02-19 | 2018-01-02 | Intervet Inc. | Swine virus vaccines that are liquid stable |
US10064404B2 (en) | 2014-06-10 | 2018-09-04 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
US10772319B2 (en) | 2014-06-10 | 2020-09-15 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
US11672247B2 (en) | 2014-06-10 | 2023-06-13 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
US10568317B2 (en) | 2015-12-08 | 2020-02-25 | Biomatrica, Inc. | Reduction of erythrocyte sedimentation rate |
US11116205B2 (en) | 2015-12-08 | 2021-09-14 | Biomatrica, Inc. | Reduction of erythrocyte sedimentation rate |
Also Published As
Publication number | Publication date |
---|---|
CH653703A5 (en) | 1986-01-15 |
SE8204148D0 (en) | 1982-07-05 |
JPS589688A (en) | 1983-01-20 |
DE3225250A1 (en) | 1983-01-20 |
SE8204148L (en) | 1983-01-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4451569A (en) | Stable enzyme composition | |
Pinto et al. | The effect of age and sex on glutathione reductase and glutathione peroxidase activities and on aerobic glutathione oxidation in rat liver homogenates | |
WIELAND et al. | Kinetic and regulatory properties of heart muscle pyruvate dehydrogenase | |
Cleland et al. | [7] Isocitrate dehydrogenase (TPN-specific) from pig heart | |
Bishop et al. | Rapid activation-inactivation of liver uridine diphosphate glucose-glycogen transferase and phosphorylase by insulin and glucagon in vivo | |
Srivastava et al. | Hyperglycemia-induced activation of human erythrocyte aldose reductase and alterations in kinetic properties | |
Storey et al. | Enzymes of energy metabolism from a vertebrate facultative anaerobe, Pseudemys scripta: Turtle heart phosphofructokinase | |
Sun et al. | Metabolism of prostaglandins in the kidney | |
Benziman | Factors affecting the activity of pyruvate kinase of Acetobacter xylinum | |
Iwata et al. | The purification and properties of aldose reductase from rat ovary | |
Calissano et al. | Control of haem synthesis by feedback inhibition on human-erythrocyte δ-aminolaevulate dehydratase | |
Kinoshita et al. | The direct oxidative carbohydrate cycle of bovine corneal epithelium | |
Jedziniak et al. | Lens polyol dehydrogenase | |
Baxter et al. | Preparation and assay of D-erythrose 4-phosphate | |
Knowles et al. | Pyruvate dehydrogenase activity in rat liver during development | |
US5705356A (en) | Reagent for invitro diagnostic determination of bicarbonate | |
Mabjeesh et al. | Low-affinity. gamma.-aminobutyric acid transport in rat brain | |
Wood et al. | Isolation of the Subunits of Transcarboxylase and Reconstitution of the Active Enzyme from the Subunits | |
Metzger et al. | A study of glucose and xylose oxidation catalyzed by the glucose-6-phosphate dehydrogenase of rat liver cytosol | |
Behm et al. | Regulatory properties of a partially purified preparation of pyruvate kinase from Fasciola hepatica | |
Veiga | Polyol dehydrogenases in Candida albicans II. Xylitol oxidation to D-xylulose | |
Klebanoff | Glutathione metabolism. 2. The oxidation and reduction of glutathione in intact erythrocytes | |
Ruiz-Herrera et al. | Purification and properties of a disulfide reductase obtained from Achromobacter starkeyi | |
Chakravorty et al. | [33a] Polyol dehydrogenases of Candida utilis: I. DPN-linked dehydrogenase | |
JPS5925684A (en) | Stable p-hydroxybenzoic acid hydroxylase composition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: TOYO BOSEKI KABUSHIKI KAISHA; 2-8., DOJIMA HAMA 2- Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:KOBAYASHI, SETSUO;BEPPU, KAZUO;SHIMIZU, HIROSHI;AND OTHERS;REEL/FRAME:004022/0524 Effective date: 19820615 Owner name: TOYO BOSEKI KABUSHIKI KAISHA, A CORP OF, JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:KOBAYASHI, SETSUO;BEPPU, KAZUO;SHIMIZU, HIROSHI;AND OTHERS;REEL/FRAME:004022/0524 Effective date: 19820615 |
|
STCF | Information on status: patent grant |
Free format text: PATENTED CASE |
|
FPAY | Fee payment |
Year of fee payment: 4 |
|
FEPP | Fee payment procedure |
Free format text: PAYOR NUMBER ASSIGNED (ORIGINAL EVENT CODE: ASPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
FPAY | Fee payment |
Year of fee payment: 8 |
|
FPAY | Fee payment |
Year of fee payment: 12 |