US5198927A - Adapter for microscope - Google Patents
Adapter for microscope Download PDFInfo
- Publication number
- US5198927A US5198927A US07/410,163 US41016389A US5198927A US 5198927 A US5198927 A US 5198927A US 41016389 A US41016389 A US 41016389A US 5198927 A US5198927 A US 5198927A
- Authority
- US
- United States
- Prior art keywords
- light
- light path
- microscope
- objective lens
- passing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000005284 excitation Effects 0.000 claims abstract description 22
- 230000008878 coupling Effects 0.000 claims description 47
- 238000010168 coupling process Methods 0.000 claims description 47
- 238000005859 coupling reaction Methods 0.000 claims description 47
- 238000001914 filtration Methods 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 22
- 229910052736 halogen Inorganic materials 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 5
- 201000004792 malaria Diseases 0.000 claims description 5
- 239000000427 antigen Substances 0.000 claims description 4
- 102000036639 antigens Human genes 0.000 claims description 4
- 108091007433 antigens Proteins 0.000 claims description 4
- 201000006353 Filariasis Diseases 0.000 claims description 3
- 241000002163 Mesapamea fractilinea Species 0.000 claims description 3
- 230000002055 immunohistochemical effect Effects 0.000 claims description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 10
- 150000002367 halogens Chemical class 0.000 claims 5
- 239000010453 quartz Substances 0.000 claims 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 5
- 238000002372 labelling Methods 0.000 claims 2
- 238000000799 fluorescence microscopy Methods 0.000 abstract description 5
- 208000035473 Communicable disease Diseases 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 230000003460 anti-nuclear Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 208000037972 tropical disease Diseases 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Definitions
- the present invention concerns an adapter for an objective lens of a microscope. More particularly, the present invention relates to an adapter which converts an ordinary microscope into a fluorescent microscope.
- Fluorescence microscopy detects a fluorescent compound to identify microscopic structures. Commonly, the fluorescent compound is attached to an antibody, and the antibody attaches itself to a corresponding antigen on a cell. The cell is then examined under a fluorescence microscope. Fluorescent areas within the cell indicate the presence and location of the antigen.
- fluorescent microscopy is a very sensitive technique, its use is limited by the expense of the specialized equipment required.
- Current fluorescent microscopes are designed specifically to provide for fluorescence illumination and filtering. Most use a high pressure mercury vapor lamp as a source of illumination. The lamps themselves are expensive, and they require a complex power supply. The illumination source occupies about as much room as the microscope itself and generates substantial heat. Due to the specialized equipment and complexity, fluorescent microscopes generally cost two to four times the cost of corresponding professional quality bench microscopes. The light source and power also make them more bulky, occupying substantially more bench space than conventional microscopes.
- the present invention concerns an adapter which can be attached (so that it can be readily detached) between the objective lens and the body of a microscope, which allows the microscope to be used for fluorescence examination.
- Professional quality microscopes in common use have removable objective lenses with standard threads.
- the focusing mechanism of such microscopes allows for the mounting threads for the objective lens (or lenses) to be moved away from the stage to make room for the inventive adapter.
- the present invention concerns an adapter to be coupled to an objective lens of a microscope
- a body member having a first (detachable) coupling means at one end thereof to receive an objective lens of a microscope and having a second (detachable) coupling means at the other end thereof to receive a microscope component.
- the body member has an axial light path running longitudinally therethrough and has a radial light path which intersects the axial light path.
- a source of light is disposed in the radial light path of the body member.
- a first filtering means is disposed in the radial light path of the body member, downstream of the source of light, for passing light at wavelengths which excite fluorescence in a specimen to be examined in a microscope and to remove light at wavelengths where visible fluorescence emission occurs in the specimen.
- a reflecting means is disposed in the intersection of the axial light path and radial light path.
- the reflecting means passes light through the axial light path and reflects light at excitation wavelengths, but does not reflect light at emission wavelengths.
- a second filtering means is disposed in the axial light path and is disposed above the reflecting means adjacent the second coupling means (the second filtering means is disposed between the reflecting means and the eye piece of a microscope in which the adapter is installed, i.e., the second filtering means is disposed between the reflecting means and the observer's eye).
- the second filtering means blocks excitation wavelengths which pass through the reflecting means.
- the present invention also concerns a combination of an adapter as described above and a microscope.
- the microscope comprises a vertical body tube, an eye piece disposed at one end of the body tube, an objective lens disposed at the other end of the body tube and a means for retaining a specimen plate under the objective lens.
- the adapter is coupled to the body tube and to the objective lens of the microscope.
- the adapter is detachably coupled to the body tube.
- the turret is detachably coupled to the objective lens.
- the adapter is aligned so as to form a longitudinal axial light path through the body tube, the adapter and the objective lens.
- the adapter for the aforesaid combination comprises a body member having a first coupling means at one end thereof to receive the objective lens and has a second coupling means at the other end thereof to receive the body tube.
- the body member has an axial light path running longitudinally therethrough and has a radial light path which intersects the axial light path.
- a source of light is disposed in the radial light path.
- a first filtering means is disposed in the radial light path, downstream of the source of light, for passing light at wavelengths which excite fluorescence in a specimen to be examined in the microscope and to remove light at wavelengths where visible fluorescence emission occurs in the specimen.
- a reflecting means is disposed in the intersection of the axial light path and radial light path.
- the reflecting means passes light through the axial light path and reflects light at excitation wavelengths, but does not reflect light at emission wavelengths.
- a second filtering means is disposed in the axial light path and is disposed between the reflecting means and the eye piece.
- the second filtering means blocks excitation wavelengths which pass through the reflecting means.
- the present invention is particularly useful as a relatively inexpensive means for detecting infectious diseases, such as tropical diseases and particularly malaria, which detection does not require the high resolution afforded by expensive fluorescent microscopes. Accordingly, in third world countries where the cost of fluorescent microscopes are prohibitive, the present invention would serve to provide medical personnel with a relatively inexpensive adapter which can be utilized in a conventional microscope in order to detect infectious diseases, such as malaria in a specimen of a patient.
- the invention can also be used to detect other blood borne parasites, for example, filariasis and heartworm in dogs. Still further, the invention can be employed in immunohistochemical procedures.
- immunohistochemical is meant a procedure where an antibody, for example, is attached to a cell or tissue, and is tagged with a fluorescent moiety and the invention can be used to detect the point of attachment.
- an antibody for example, is attached to a cell or tissue, and is tagged with a fluorescent moiety and the invention can be used to detect the point of attachment.
- One example of such procedure is to detect an anti-nuclear antibody which is tagged with fluorescein.
- FIG. 1 depicts a side elevational view of a conventional microscope wherein the inventive adapter can be employed
- FIG. 2 is a side elevational view of a conventional microscope as depicted in FIG. 1 and having installed therein an adapter according to the present invention
- FIG. 3 is an elevational view of a portion of the microscope and adapter as depicted in FIG. 2 and containing a partial cross-sectional view taken along line 3-3 in FIG. 2;
- FIG. 4 is an elevational view of a microscope adapter as depicted in FIG. 2 and FIG. 3 in a detached state from a turret and an objective lens of a microscope as depicted in FIGS. 2 and 3.
- FIG. 1 depicts a conventional microscope 10 in which an inventive adapter can be utilized.
- Microscope 10 has eye piece 12, turret 14, objective lens 16, objective lens 18 and specimen holder 20.
- FIG. 2 A preferred microscope adapter 22 of the invention is depicted in FIG. 2.
- the adapter 22 is installed in a microscope 10 between objective lens 18 and turret 14.
- the adapter 22 is depicted in greater detail in FIG. 3.
- the light source for microscope adapter 22 includes a quartz-halogen lamp 24 and a condensing lens 26.
- Non-limiting examples of other lamps that can be employed are a vacuum tungsten lamp or a mercury lamp.
- Light from this source is filtered by an excitation filter 28 so as to pass light at wavelengths (for example, 490 nm and below) which excite fluorescence in the specimen to be examined, typically in the violet region, and to remove light at wavelengths (for example, 510 nm and above) where visible fluorescence emission occurs in the specimen, typically green, yellow or red regions.
- the filtered excitation light is then reflected by a dichroic mirror 30 onto the objective lens 18 of the microscope, which focuses the excitation light onto the specimen.
- the dichroic mirror 30 reflects the majority of light (preferably reflects 80% and greater and more particularly, reflects 90% or greater) at the excitation wavelengths, but does not reflect light at emission wavelengths.
- a partially reflective mirror could be used, but it is substantially less efficient.
- a beam splitter typically reflects and transmits about 50% of light.
- Fluorescent light emitted by the specimen is focused by the objective lens 18, and passes through the mirror 30 eventually to the eye piece (see item 12 in FIG. 2).
- An emission filter 32 which passes emission wavelengths and blocks excitation wavelengths is placed in the light path between the mirror 30 and the observer to block any reflections of the excitation beam from the specimen or the surrounding optics. It is convenient to place this emission filter 32 just above the dichroic mirror 30, i.e., the emission filter 32 is disposed between the dichroic mirror 30 and the eye piece of a microscope in which the adapter is installed. As depicted in FIG. 3, emission filter 32 is adjacent to screw threads 38.
- Screw threads 34 permit the adapter 22 to be screw thread connected directly to the screw threads 36 of the objective lens 18 of the microscope.
- Screw threads 38 of adapter 22 permit the adapter 22 to be screw thread connected directly to the screw threads 40 of the turret 14 of the microscope.
Abstract
Description
Claims (16)
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/410,163 US5198927A (en) | 1989-09-20 | 1989-09-20 | Adapter for microscope |
ZA902526A ZA902526B (en) | 1989-09-20 | 1990-04-02 | Adapter for microscope |
MX020412A MX174253B (en) | 1989-09-20 | 1990-04-23 | MICROSCOPE ADAPTER |
CA002015228A CA2015228C (en) | 1989-09-20 | 1990-04-24 | Adapter for microscope |
EP90109565A EP0418471B1 (en) | 1989-09-20 | 1990-05-19 | Adapter for microscope |
AT90109565T ATE129817T1 (en) | 1989-09-20 | 1990-05-19 | ADAPTER FOR MICROSCOPE. |
DE69023309T DE69023309T2 (en) | 1989-09-20 | 1990-05-19 | Adapter for microscope. |
BR909003215A BR9003215A (en) | 1989-09-20 | 1990-07-06 | ADAPTER TO BE COUPLED TO A MICROSCOPE AND MICROSCOPE OBJECTIVE |
KR1019900010243A KR910006746A (en) | 1989-09-20 | 1990-07-06 | Adapter for microscope |
CN90107730A CN1024952C (en) | 1989-09-20 | 1990-09-15 | Adapter for microscope |
US07/986,864 US5349468A (en) | 1989-09-20 | 1992-12-04 | Adapter for microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/410,163 US5198927A (en) | 1989-09-20 | 1989-09-20 | Adapter for microscope |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/986,864 Division US5349468A (en) | 1989-09-20 | 1992-12-04 | Adapter for microscope |
Publications (1)
Publication Number | Publication Date |
---|---|
US5198927A true US5198927A (en) | 1993-03-30 |
Family
ID=23623503
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/410,163 Expired - Lifetime US5198927A (en) | 1989-09-20 | 1989-09-20 | Adapter for microscope |
US07/986,864 Expired - Lifetime US5349468A (en) | 1989-09-20 | 1992-12-04 | Adapter for microscope |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/986,864 Expired - Lifetime US5349468A (en) | 1989-09-20 | 1992-12-04 | Adapter for microscope |
Country Status (10)
Country | Link |
---|---|
US (2) | US5198927A (en) |
EP (1) | EP0418471B1 (en) |
KR (1) | KR910006746A (en) |
CN (1) | CN1024952C (en) |
AT (1) | ATE129817T1 (en) |
BR (1) | BR9003215A (en) |
CA (1) | CA2015228C (en) |
DE (1) | DE69023309T2 (en) |
MX (1) | MX174253B (en) |
ZA (1) | ZA902526B (en) |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5349468A (en) * | 1989-09-20 | 1994-09-20 | Yale University | Adapter for microscope |
US5646781A (en) * | 1995-05-15 | 1997-07-08 | Omega Optical, Inc. | Optical filters for forming enhanced images |
US6023368A (en) * | 1997-10-24 | 2000-02-08 | Samsung Electronics Co., Ltd. | Illumination system for use with an optical instrument having an auxiliary magnifying lens |
US6197523B1 (en) | 1997-11-24 | 2001-03-06 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer and/or hematologic progenitor cells in whole blood |
US6313944B2 (en) * | 1997-03-10 | 2001-11-06 | Nikon Corporation | Microscope turret assembly and a microscope |
US20050239058A1 (en) * | 1997-11-24 | 2005-10-27 | Rimm David L | Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood |
US20060092503A1 (en) * | 2004-10-26 | 2006-05-04 | University Of Maryland, Baltimore | Portable microscope with detachable portion |
US20070153372A1 (en) * | 2006-01-03 | 2007-07-05 | Physical Sciences, Inc. | Fluorescence illumination method and apparatus for stereomicroscopes |
US20100097692A1 (en) * | 2008-10-22 | 2010-04-22 | Microbrightfield, Inc. | Movable Objective Lens Assembly for an Optical Microscope and Optical Microscopes Having Such an Assembly |
US20100118549A1 (en) * | 2008-11-07 | 2010-05-13 | Endure Medical, Inc. | Stereoscopic Illumination System for Microscope |
WO2012037074A1 (en) | 2010-09-14 | 2012-03-22 | Qbc Diagnostics, Inc. | Adaptor for microscopes |
US20140368816A1 (en) * | 2012-02-10 | 2014-12-18 | The Board Of Trustees Of The Leland Stanford Junior University | Optical device |
WO2015092778A1 (en) * | 2013-12-21 | 2015-06-25 | Isis Innovation Ltd. | Light source adaptor for a microscope |
CN106442324A (en) * | 2016-11-01 | 2017-02-22 | 昆明泰来光学科技开发有限公司 | Multi-waveband LED reflected/transmitted illumination objective table |
US9810892B2 (en) | 2013-03-15 | 2017-11-07 | The Board Of Trustees Of The Leland Stanford Junior University | Optical lens fabrication |
US20210011267A1 (en) * | 2018-04-18 | 2021-01-14 | Carl Zeiss Microscopy Gmbh | Adapter for an objective lens, device for supporting the adapter and positioning device |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2251701A (en) * | 1990-12-01 | 1992-07-15 | K W Kirk & Sons Limited | UV Microscope illuminator |
US5805342A (en) * | 1995-10-31 | 1998-09-08 | Gravely; Benjamin T. | Imaging system with means for sensing a filtered fluorescent emission |
AU4562497A (en) * | 1996-10-02 | 1998-04-24 | Cell Robotics, Inc. | Microscope with laser port |
US6661573B1 (en) * | 2001-09-17 | 2003-12-09 | Digilab, Llc | Infrared microscope adapter for viewing at an angle |
US7359116B2 (en) * | 2001-10-16 | 2008-04-15 | Hamilton Thome Biosciences, Inc. | Microscope turret mounted laser EPI-illumination port |
US9335532B2 (en) * | 2001-10-16 | 2016-05-10 | Hamilton Thorne, Inc. | Laser assembly for use with a microscope |
US7538939B2 (en) * | 2002-10-31 | 2009-05-26 | Leica Microsystems (Schweiz) Ag | Stereomicroscope or additional element for a stereomicroscope |
CZ2006352A3 (en) * | 2006-05-31 | 2007-12-12 | Ústav prístrojové techniky AV CR, v.v.i. | Device for connecting radiation source to microscope objective |
JP5296686B2 (en) | 2006-08-14 | 2013-09-25 | ウエストオーバー サイエンティフィック,インコーポレイテッド | Semiconductor fluorescent assembly and microscope |
WO2008156475A1 (en) * | 2007-06-15 | 2008-12-24 | Hamilton Thorne Biosciences, Inc. | Laser assembly for use with a microscope |
KR20090082778A (en) * | 2008-01-28 | 2009-07-31 | 삼성모바일디스플레이주식회사 | Organic light emitting diode and manufacturing thereof |
US8149504B2 (en) * | 2008-06-10 | 2012-04-03 | Hamilton Thorne Biosciences, Inc. | Optical indicator for microscopic laser beam manipulation |
JP5551179B2 (en) * | 2008-11-07 | 2014-07-16 | ハミルトン−ソーン インコーポレイテッド | Modular objective lens assembly |
DE102009037841B4 (en) | 2009-08-18 | 2020-01-23 | Carl Zeiss Meditec Ag | Optical system with wavefront analysis system and assembly with wavefront analysis system for a microscope with microscope chassis |
JP6637350B2 (en) * | 2016-03-16 | 2020-01-29 | オリンパス株式会社 | Upright microscope |
EP3513238A4 (en) * | 2016-09-13 | 2020-08-12 | Inscopix, Inc. | Adapter for microscopic imaging |
EP3537997A4 (en) | 2016-11-14 | 2020-09-23 | Gynion, LLC | System and method for delivering therapeutic agents to the uterine cavity |
CN106873143A (en) * | 2017-04-27 | 2017-06-20 | 广西奥顺仪器有限公司 | A kind of built-in camera and light splitting interface structure |
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US1837993A (en) * | 1930-03-17 | 1931-12-22 | Bausch & Lomb | Microscope eye piece tube |
DE581687C (en) * | 1929-10-29 | 1933-08-01 | August Hirt Dr | Method for microscopic examination of living objects |
US2004806A (en) * | 1933-02-02 | 1935-06-11 | Bausch & Lomb | Microscope illuminator |
US2071408A (en) * | 1935-01-28 | 1937-02-23 | Charles F Jones | Microscope |
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US2130493A (en) * | 1934-08-03 | 1938-09-20 | Leitz Ernst Gmbh | Illuminating device for microscopes |
US2471879A (en) * | 1946-02-08 | 1949-05-31 | American Optical Corp | Vertical illuminator |
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US5198927A (en) * | 1989-09-20 | 1993-03-30 | Yale University | Adapter for microscope |
-
1989
- 1989-09-20 US US07/410,163 patent/US5198927A/en not_active Expired - Lifetime
-
1990
- 1990-04-02 ZA ZA902526A patent/ZA902526B/en unknown
- 1990-04-23 MX MX020412A patent/MX174253B/en unknown
- 1990-04-24 CA CA002015228A patent/CA2015228C/en not_active Expired - Fee Related
- 1990-05-19 AT AT90109565T patent/ATE129817T1/en not_active IP Right Cessation
- 1990-05-19 DE DE69023309T patent/DE69023309T2/en not_active Expired - Fee Related
- 1990-05-19 EP EP90109565A patent/EP0418471B1/en not_active Expired - Lifetime
- 1990-07-06 BR BR909003215A patent/BR9003215A/en not_active Application Discontinuation
- 1990-07-06 KR KR1019900010243A patent/KR910006746A/en not_active Application Discontinuation
- 1990-09-15 CN CN90107730A patent/CN1024952C/en not_active Expired - Fee Related
-
1992
- 1992-12-04 US US07/986,864 patent/US5349468A/en not_active Expired - Lifetime
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Also Published As
Publication number | Publication date |
---|---|
KR910006746A (en) | 1991-04-30 |
CN1024952C (en) | 1994-06-08 |
ATE129817T1 (en) | 1995-11-15 |
BR9003215A (en) | 1991-08-27 |
ZA902526B (en) | 1991-10-30 |
EP0418471A2 (en) | 1991-03-27 |
MX174253B (en) | 1994-05-02 |
DE69023309T2 (en) | 1996-04-18 |
EP0418471A3 (en) | 1991-09-18 |
DE69023309D1 (en) | 1995-12-07 |
EP0418471B1 (en) | 1995-11-02 |
US5349468A (en) | 1994-09-20 |
CA2015228A1 (en) | 1991-03-20 |
CN1052557A (en) | 1991-06-26 |
CA2015228C (en) | 2000-01-25 |
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