US7653492B2 - Method of reducing the effect of direct interference current in an electrochemical test strip - Google Patents
Method of reducing the effect of direct interference current in an electrochemical test strip Download PDFInfo
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- US7653492B2 US7653492B2 US10/577,586 US57758604A US7653492B2 US 7653492 B2 US7653492 B2 US 7653492B2 US 57758604 A US57758604 A US 57758604A US 7653492 B2 US7653492 B2 US 7653492B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/49—Systems involving the determination of the current at a single specific value, or small range of values, of applied voltage for producing selective measurement of one or more particular ionic species
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1486—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using enzyme electrodes, e.g. with immobilised oxidase
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/150022—Source of blood for capillary blood or interstitial fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
- A61B5/150274—Manufacture or production processes or steps for blood sampling devices
- A61B5/150282—Manufacture or production processes or steps for blood sampling devices for piercing elements, e.g. blade, lancet, canula, needle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150358—Strips for collecting blood, e.g. absorbent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150412—Pointed piercing elements, e.g. needles, lancets for piercing the skin
- A61B5/150435—Specific design of proximal end
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150503—Single-ended needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Abstract
Description
where G is the corrected current density, WE1 is the uncorrected current density at the first working electrode, WE2 is the uncorrected current density at the second working electrode, Acov is the coated area of the second working electrode, and Aunc is the uncoated area of the second working electrode 2.
where f1 is equal to
f2 is equal to
Aunc1 is the uncoated area of the first working electrode; Aunc2 is the uncoated area of the second working electrode; Acov1 is the coated area of said first working electrode; Acov2 is the coated area of the second working electrode; G is the corrected current value; WE1 is the uncorrected current density at the first working electrode; and WE2 is the uncorrected current density at the second working electrode.
WE 1 =G+I cov (Eq 1)
where WE1 is the current density at first working electrode, G is the current density due to glucose which is independent of interferences, and Icov is the current density due to interferences at the portion of a working electrode covered with reagent.
WE 2 =G+I cov +I unc (Eq 2)
where WE2 is the current density at second working electrode and Iunc is the current density due to interferences at the portion of a working electrode not covered with reagent. Alternative embodiments of the present invention can be made using different areas of reagent coating for the first and second working electrode, but then the equations must account for the different uncoated areas.
where Acov is the area of second working electrode covered with reagent and Aunc is the area of second working electrode not covered with reagent.
I cov =ƒ×I unc (Eq 3b)
where ƒ is a correction factor which incorporates the effects of the interferent oxidation efficiency of the coated to uncoated portion.
G=WE 1 −I cov (Eq 4)
Next, Icov from Equation 3a can be substituted into Equation 4 to yield
Next,
I unc =WE 2 −WE 1 (Eq 6)
Next, Iunc from Equation 6 can be substituted into
may be programmed into a glucose meter, in, for example, a read only memory. In another embodiment of the present invention, the proportion
may be transferred to the meter via a calibration code chip which would may account for manufacturing variations in Acov or Aunc.
G=WE 1−{ƒ×(WE 2 −WE 1)} (Eq 7b)
where
Aunc1=is an uncoated area of the first working electrode, Aunc2=is an uncoated area of the second working electrode, Acov1=is a coated area of the first working electrode, and Acov2=is a coated area of the second working electrode.
TABLE 1 |
Summary of Interference Performance Using |
Uncorrected and Corrected Current Output |
Glucose | Inteferent | Normal | ||
Concen- | Concentration | Concentration | ||
tration | where effect | range of | ||
Mode | Interferent | (mg/dL) | is signficant | interferent |
Uncorrected | Acetaminophen | 70 | 11 | 1-2 |
Uncorrected | Gentisic Acid | 70 | 10 | 0.05-0.5 |
Uncorrected | Uric Acid | 70 | 5 | 2.6-7.2 |
Uncorrected | Acetaminophen | 240 | 16 | 1-2 |
Uncorrected | Gentisic Acid | 240 | 12 | 0.05-0.5 |
Uncorrected | Uric Acid | 240 | 8 | 2.6-7.2 |
Corrected | Acetaminophen | 70 | 120 | 1-2 |
Corrected | Gentisic Acid | 70 | 47 | 0.05-0.5 |
Corrected | Uric Acid | 70 | 33 | 2.6-7.2 |
Corrected | Acetaminophen | 240 | 59 | 1-2 |
Corrected | Gentisic Acid | 240 | 178 | 0.05-0.5 |
Corrected | Uric Acid | 240 | 29 | 2.6-7.2 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/577,586 US7653492B2 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51625203P | 2003-10-31 | 2003-10-31 | |
US55872804P | 2004-03-31 | 2004-03-31 | |
US55842404P | 2004-03-31 | 2004-03-31 | |
PCT/GB2004/004574 WO2005045412A1 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
US10/577,586 US7653492B2 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
Publications (2)
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US20070276621A1 US20070276621A1 (en) | 2007-11-29 |
US7653492B2 true US7653492B2 (en) | 2010-01-26 |
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ID=34577659
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
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US10/977,292 Abandoned US20050114062A1 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
US10/577,586 Active 2024-12-30 US7653492B2 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
US10/977,316 Abandoned US20050139469A1 (en) | 2003-10-31 | 2004-10-29 | Electrochemical test strip for reducing the effect of direct and mediated interference current |
US10/977,086 Abandoned US20050139489A1 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct and mediated interference current in an electrochemical test strip |
US10/977,154 Expired - Fee Related US7618522B2 (en) | 2003-10-31 | 2004-10-29 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
US10/976,489 Abandoned US20050133368A1 (en) | 2003-10-31 | 2004-10-29 | Electrochemical test strip for reducing the effect of direct interference current |
US12/574,469 Abandoned US20100018878A1 (en) | 2003-10-31 | 2009-10-06 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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US10/977,292 Abandoned US20050114062A1 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct interference current in an electrochemical test strip |
Family Applications After (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/977,316 Abandoned US20050139469A1 (en) | 2003-10-31 | 2004-10-29 | Electrochemical test strip for reducing the effect of direct and mediated interference current |
US10/977,086 Abandoned US20050139489A1 (en) | 2003-10-31 | 2004-10-29 | Method of reducing the effect of direct and mediated interference current in an electrochemical test strip |
US10/977,154 Expired - Fee Related US7618522B2 (en) | 2003-10-31 | 2004-10-29 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
US10/976,489 Abandoned US20050133368A1 (en) | 2003-10-31 | 2004-10-29 | Electrochemical test strip for reducing the effect of direct interference current |
US12/574,469 Abandoned US20100018878A1 (en) | 2003-10-31 | 2009-10-06 | Method of reducing interferences in an electrochemical sensor using two different applied potentials |
Country Status (16)
Country | Link |
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US (7) | US20050114062A1 (en) |
EP (6) | EP1678490B1 (en) |
JP (6) | JP2007514931A (en) |
KR (5) | KR20070027496A (en) |
AT (4) | ATE354796T1 (en) |
AU (6) | AU2004288004B2 (en) |
CA (6) | CA2544424A1 (en) |
DE (4) | DE602004021835D1 (en) |
DK (3) | DK1685393T3 (en) |
ES (4) | ES2285536T3 (en) |
HK (4) | HK1091896A1 (en) |
IL (6) | IL175324A0 (en) |
PL (3) | PL1678489T3 (en) |
PT (2) | PT1678489E (en) |
SG (2) | SG131942A1 (en) |
WO (6) | WO2005045416A1 (en) |
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