WO1987003825A1 - Transfer device for serological diagnosis - Google Patents
Transfer device for serological diagnosis Download PDFInfo
- Publication number
- WO1987003825A1 WO1987003825A1 PCT/IT1986/000092 IT8600092W WO8703825A1 WO 1987003825 A1 WO1987003825 A1 WO 1987003825A1 IT 8600092 W IT8600092 W IT 8600092W WO 8703825 A1 WO8703825 A1 WO 8703825A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- vessels
- transfer device
- plate
- duct
- serum
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
Definitions
- the present invention provides a transfer device for serological diagnosis, to be used in particular in the diagnostic processes for HTLV III virus infections.
- the transfer device allows to interconnect a plurality of opposite vessels, con ⁇ taining each a small ball coated with antigens, so as to allow, by a single operation, to shift the serum under examination from a vessel to the other so quickly that the liquid temperature has no time to diminish, and absolutely avoiding any contaminations and/or possi ⁇ bility of mistakes.
- the taken sample is introduced in a vessel in contact with a solid substrate, for instance a polystyrene ball covered with antibodies, to which immunoglobulins adhere that are then detected by means of conjugated anti-antibodies .
- a solid substrate for instance a polystyrene ball covered with antibodies, to which immunoglobulins adhere that are then detected by means of conjugated anti-antibodies .
- the colouring of the ball indicates the presence in blood of the sought for immunoglobulins and, accordingly, the infection state.
- the critical phase of this new process is the elution, that takes place at a temperature oscillating between 50° and 58°C, necessary to cause the specific immunoglo ⁇ bulins of the various classes - caught by the antigens secured to the first substrate - to detach therefrom, which enables the production of an eluate of specific antibodies of the various classes, with few non-speci- fie antibodies .
- the transfer of the eluate from the first to the second vessel takes l ce via suction and force-pumps.
- the present invention provides a transfer device consisting substa tially of a plurality of con ⁇ necting elements, fastened to a common support, and arranged in such a manner as to fit, from either side, to the plates carrying the vessels used at present in laboratories for serological diagnosis.
- a characteristic of the invention is the special shap ⁇ ing of its parts that allows to arrange a pair of opposi ' te plates, so that each vessel of one of these plates communicates with the corresponding vessel of the other plate, the whole being watertight; it is thus possible, by a single operation, to shift serum from the vessels in one plate to those in the second plate, without any decrease in temperature taking place.
- FIG. 1 shows the plane view of the transfer device according to the invention
- FIG. 2 shows the side, partly section view, of a transfer device according to the invention
- FIG. 3 shows a view from above of a detail of the transfer device according to the invention.
- a plate or support 1 comprises a plurality of elements, each of which is referenced as a whole with No. 2, each of them being suitable to allow connection between a pair of opposite vessels.
- elements 2 are located at the vertexes of a square link grid.
- Each element 2 is substantially a hollow cylinder defin ⁇ ed by side walls 3, inside which passes the serum, that shifts from one vessel to the other.
- each element 2 is not of constant size but has a narrowing at the central zone.
- the upper inner part of element 2 comprises a first, substantially cylindrical section 4, linked to a frusto-conical section 5 and to a lower duct 6, of lesser diameter.
- the sizes of the frusto-conical section 5 too are inferior to those of the upper cylindrical section 4, so as to define, on the bottom of the latter, a flat peripheral edge 7.
- the lower duct 6 extends downwards to a certain extent
- Plate 1 comprises further a number of stif ening ribs 12. The utilization takes place as follows:
- the samples of serum to be examined are inside as many vessels 13 (Fig.2) , present on a support plate and arranged in the same way as the elements 2.
- Each of these vessels contains, in addition to serum, a small
- the transfer device according to the invention is put on top o the essels, so that their ' edge fits the
- the transfer device can be inserted between a pair of opposite twin plates, provided each with a plurality of vessels, in such a way as to ensure a continuous link between each vessel of one plate and the corresponding vessel of the other, the said link being direct and watertight.
Abstract
A transfer device for serological diagnosis, wherein there are provided means (1) suitable to fit between a pair of opposite plates each equipped with a plurality of vessels (13, 14) intended for containing the serum under exam, the said means (1) constituting a link between pairs of opposite vessels (13, 14), such as to allow the transfer of serum from the vessels (13) in one plate to the vessels (14) in the other plate by turning the whole upside-down.
Description
"Z_i _i_-_I-i_l_£-ιXI----_£2_i--_._._i_-_-2_-I_-Δ_-__-IΔ_-_i£_iI_-"
The present invention provides a transfer device for serological diagnosis, to be used in particular in the diagnostic processes for HTLV III virus infections.
The transfer device according to the invention allows to interconnect a plurality of opposite vessels, con¬ taining each a small ball coated with antigens, so as to allow, by a single operation, to shift the serum under examination from a vessel to the other so quickly that the liquid temperature has no time to diminish, and absolutely avoiding any contaminations and/or possi¬ bility of mistakes.
As it is known, one of the most widely used methods for the laboratory diagnosis of infections is based on the search for specific antibodies through the examination of the subject's- blood samples.
The taken sample is introduced in a vessel in contact with a solid substrate, for instance a polystyrene ball
covered with antibodies, to which immunoglobulins adhere that are then detected by means of conjugated anti-antibodies .
The colouring of the ball, further to the reaction with such anti-antibodies, indicates the presence in blood of the sought for immunoglobulins and, accordingly, the infection state.
However, the above mentioned methods, although having a good sensitivity, present an often not sufficiently high specificity.
To this effect it has been recently developed a diagno- sis method, which was the object of a separate applica¬ tion by the same Applicant, wherein serum, after immer¬ sion in a vessel containing the antigen-coated substra¬ te, is heated at the end of incubation and the eluate is taken and put in a second vessel, containing in its turn a substrate coated with antigens of the same kind; to them fix the immunoglobulins, that are then detected by the prior art methods.
The critical phase of this new process is the elution, that takes place at a temperature oscillating between 50° and 58°C, necessary to cause the specific immunoglo¬ bulins of the various classes - caught by the antigens secured to the first substrate - to detach therefrom, which enables the production of an eluate of specific antibodies of the various classes, with few non-speci-
fie antibodies .
It is absolutely necessary that the eluate is introduc¬ ed in the vessel that contains the second antigen-coat- ed ball without the temperature decreasing, because in that case the antibodies would be absorbed again by the antigens present on the first substrate.
According to the known technique, the transfer of the eluate from the first to the second vessel takes l ce via suction and force-pumps.
It clearly appears, therefore, that the problem connect¬ ed with the carrying out of this new diagnosis method consists in the difficulty of transferring the eluate from a vessel to the other quickly enough as to avoid any decrease in temperature, without any contamination of the eluate and with minimum possibility of mistakes.
To this end the present invention provides a transfer device consisting substa tially of a plurality of con¬ necting elements, fastened to a common support, and arranged in such a manner as to fit, from either side, to the plates carrying the vessels used at present in laboratories for serological diagnosis.
A characteristic of the invention is the special shap¬ ing of its parts that allows to arrange a pair of opposi'te plates, so that each vessel of one of these plates communicates with the corresponding vessel of
the other plate, the whole being watertight; it is thus possible, by a single operation, to shift serum from the vessels in one plate to those in the second plate, without any decrease in temperature taking place.
The present invention will be now described in detail, with special reference to the enclosed drawings, in which :
- Fig. 1 shows the plane view of the transfer device according to the invention;
- Fig. 2 shows the side, partly section view, of a transfer device according to the invention;
- Fig. 3 shows a view from above of a detail of the transfer device according to the invention.
A plate or support 1 comprises a plurality of elements, each of which is referenced as a whole with No. 2, each of them being suitable to allow connection between a pair of opposite vessels.
In the drawing, elements 2 are located at the vertexes of a square link grid.
Each element 2 is substantially a hollow cylinder defin¬ ed by side walls 3, inside which passes the serum, that shifts from one vessel to the other.
The inside of each element 2 is not of constant size but has a narrowing at the central zone. With special reference to Fig. 2, the upper inner part of element 2 comprises a first, substantially cylindrical section 4,
linked to a frusto-conical section 5 and to a lower duct 6, of lesser diameter.
The sizes of the frusto-conical section 5 too are inferior to those of the upper cylindrical section 4, so as to define, on the bottom of the latter, a flat peripheral edge 7.
The lower duct 6 extends downwards to a certain extent,
10 so as to define an annular groove 8 comprised between said duct 6 and the wall 3 of elemenb 2 . -
From the wall of the frusto-conical section 5 extend inwards a number of tongues 1.0; .there is also a pin 11
15 or the like, orthogonal to the axis of 'sections 5 and 6, located at the central zone thereof.
Plate 1 comprises further a number of stif ening ribs 12. The utilization takes place as follows:
20 the samples of serum to be examined are inside as many vessels 13 (Fig.2) , present on a support plate and arranged in the same way as the elements 2. Each of these vessels contains, in addition to serum, a small
25 ball coated with the necessary antigens for the dia- gnosis .
A
The transfer device according to the invention is put on top o the essels, so that their 'edge fits the
30 annular groove 8.
Then the ball to be used in the subsequent stage - that is prevented from falling in the underlying vessels by means of pin 11 and tongues 10 - is inserted in the upper zone of each connecting element 2.
There is then placed on top of the pile a second plate provided with as many vessels that fit in elements 2 until coming in contact with the annular edge 7.
At this point it is sufficient to seize the whole unit and turn - it upside-down, to transfer serum from the lower vessels 13 to the upper vessels, indicated by No. 14.
The passage is extremely quick, so that the temperature of the eluate remains constant throughout.
The transfer device according to the in ention, there¬ fore, can be inserted between a pair of opposite twin plates, provided each with a plurality of vessels, in such a way as to ensure a continuous link between each vessel of one plate and the corresponding vessel of the other, the said link being direct and watertight.
of course the sizes, as well as the employed materials, may vary in accordance with the requirements of use.
Claims
1. A transfer device for serological diagnosis, wherein there are provided means suitable to fit between a pair of opposite plates each equipped with a plurality of vessels intended for containing the serum under exam, the said means constituting a link between pairs of opposite vessels, such as to allow the transfer of serum from the vessels in one plate to the vessels in the other plate by turning the whole upside-down.
2. Transfer device according to claim 1, wherein said means consist of a plate provided with a plurality of tubular connecting elements extending from both sides, each being suitable to receive the edges- of a pair of vessels, there being provided means operable to ensure watertightness.
3. Transfer device according to claim 2, wherein said ducts comprise a central zone having lesser diameter, so shaped as to define an annular edge at one side, and an annualr groove at the other side, both suitable to act as reference means for the vessel edges.
4. Transfer device according to claim 2, wherein each duct is provided with means fit to prevent the balls held in the vessels from passing into the vessels of the other plate.
5. Transfer device according to claim 3, wherein said central duct comprises a frusto-conical zone linked to a cylindrical zone, a pin orthogonal to the duct axis passing through the duct itself.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO873401A NO873401L (en) | 1985-12-17 | 1987-08-13 | SEROLOGICAL DIAGNOSIS TRANSFER DEVICE. |
DK424287A DK424287D0 (en) | 1985-12-17 | 1987-08-14 | TRANSMISSION DEVICE FOR USE FOR SEROLOGICAL DIAGNOSTICATION |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT24180B/85 | 1985-12-17 | ||
IT8524180U IT209360Z2 (en) | 1985-12-17 | 1985-12-17 | CONVEYOR FOR SEROLOGICAL DIAGNOSIS. |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1987003825A1 true WO1987003825A1 (en) | 1987-07-02 |
Family
ID=11212401
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IT1986/000092 WO1987003825A1 (en) | 1985-12-17 | 1986-12-16 | Transfer device for serological diagnosis |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0287565A1 (en) |
JP (1) | JPS63502774A (en) |
AU (1) | AU6838987A (en) |
DK (1) | DK424287D0 (en) |
ES (1) | ES1001194Y (en) |
IT (1) | IT209360Z2 (en) |
NO (1) | NO873401L (en) |
PT (1) | PT83950A (en) |
WO (1) | WO1987003825A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2829709A1 (en) * | 2001-09-14 | 2003-03-21 | In Cyto Tox | Apparatus for the transfer of a fluid, for in vitro testing of a number of fluid deposits from the same sample, has an adapter with an opening to transfer fluid from a holding well to a receiving well without pipetting |
US11382185B2 (en) * | 2016-01-08 | 2022-07-05 | Siemens Healthcare Diagnostics Inc. | Heating element for sensor array |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2378283A1 (en) * | 1977-01-21 | 1978-08-18 | Searle & Co | Reaction testing mould - has base plate provided with depending plasma test tubes with support feet carrying removable identification cards (BE 24.7.78) |
US4111754A (en) * | 1976-11-29 | 1978-09-05 | Hydow Park | Immunological testing devices and methods |
FR2404466A1 (en) * | 1977-10-03 | 1979-04-27 | Manudo | Sample containers for medical analysis etc. - made from two sheets of resin, receptacles in one sheet, and tubes being formed in the other |
EP0154687A2 (en) * | 1982-03-03 | 1985-09-18 | Becton Dickinson and Company | Carrier plate and screening assembly for immunoassay procedures |
-
1985
- 1985-12-17 IT IT8524180U patent/IT209360Z2/en active
-
1986
- 1986-12-16 JP JP62500401A patent/JPS63502774A/en active Pending
- 1986-12-16 WO PCT/IT1986/000092 patent/WO1987003825A1/en not_active Application Discontinuation
- 1986-12-16 EP EP87900254A patent/EP0287565A1/en not_active Withdrawn
- 1986-12-16 AU AU68389/87A patent/AU6838987A/en not_active Abandoned
- 1986-12-17 PT PT83950A patent/PT83950A/en not_active Application Discontinuation
- 1986-12-17 ES ES19868601661U patent/ES1001194Y/en not_active Expired
-
1987
- 1987-08-13 NO NO873401A patent/NO873401L/en unknown
- 1987-08-14 DK DK424287A patent/DK424287D0/en not_active Application Discontinuation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4111754A (en) * | 1976-11-29 | 1978-09-05 | Hydow Park | Immunological testing devices and methods |
FR2378283A1 (en) * | 1977-01-21 | 1978-08-18 | Searle & Co | Reaction testing mould - has base plate provided with depending plasma test tubes with support feet carrying removable identification cards (BE 24.7.78) |
FR2404466A1 (en) * | 1977-10-03 | 1979-04-27 | Manudo | Sample containers for medical analysis etc. - made from two sheets of resin, receptacles in one sheet, and tubes being formed in the other |
EP0154687A2 (en) * | 1982-03-03 | 1985-09-18 | Becton Dickinson and Company | Carrier plate and screening assembly for immunoassay procedures |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2829709A1 (en) * | 2001-09-14 | 2003-03-21 | In Cyto Tox | Apparatus for the transfer of a fluid, for in vitro testing of a number of fluid deposits from the same sample, has an adapter with an opening to transfer fluid from a holding well to a receiving well without pipetting |
US11382185B2 (en) * | 2016-01-08 | 2022-07-05 | Siemens Healthcare Diagnostics Inc. | Heating element for sensor array |
Also Published As
Publication number | Publication date |
---|---|
DK424287A (en) | 1987-08-14 |
AU6838987A (en) | 1987-07-15 |
EP0287565A1 (en) | 1988-10-26 |
PT83950A (en) | 1987-08-19 |
NO873401D0 (en) | 1987-08-13 |
IT209360Z2 (en) | 1988-10-05 |
ES1001194Y (en) | 1988-11-16 |
JPS63502774A (en) | 1988-10-13 |
ES1001194U (en) | 1988-04-16 |
NO873401L (en) | 1987-08-13 |
IT8524180V0 (en) | 1985-12-17 |
DK424287D0 (en) | 1987-08-14 |
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