WO1988007670A3 - Time-resolved fluorescence apparatus and immunoassay - Google Patents

Time-resolved fluorescence apparatus and immunoassay Download PDF

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Publication number
WO1988007670A3
WO1988007670A3 PCT/US1988/001019 US8801019W WO8807670A3 WO 1988007670 A3 WO1988007670 A3 WO 1988007670A3 US 8801019 W US8801019 W US 8801019W WO 8807670 A3 WO8807670 A3 WO 8807670A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence
time
immunoassay
optical image
current
Prior art date
Application number
PCT/US1988/001019
Other languages
French (fr)
Other versions
WO1988007670A2 (en
Inventor
Yong-Sheng Chao
Salvador M Fernandez
Ernest F Guignon
Hann-Ching P Wang
Original Assignee
Chimerix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/031,408 external-priority patent/US4923819A/en
Priority claimed from US07/031,288 external-priority patent/US4855930A/en
Application filed by Chimerix Corp filed Critical Chimerix Corp
Publication of WO1988007670A2 publication Critical patent/WO1988007670A2/en
Publication of WO1988007670A3 publication Critical patent/WO1988007670A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/069—Supply of sources
    • G01N2201/0696—Pulsed
    • G01N2201/0697—Pulsed lasers

Abstract

A method and apparatus for time-resolved fluorescence spectroscopy is described in which laser light from a single pulse is used to excite fluorescent photons in a sample (24), which fluorescence is detected by a PMT (14) optimized for linearity and response time to produce photoelectrons which generate a current at the PMT anode. This current is discharged through an R/C network to produce a voltage amplitude waveform which is converted to an optical image (26), intensified (28), stored (36) and digitized (34). The digitized version of the optical image is processed in a data processor (38) to calculate the true fluorescence impulse response. Fluorescence immunoassay for determining single or multiple analytes based upon a single measurement of fluorescence are described.
PCT/US1988/001019 1987-03-27 1988-03-24 Time-resolved fluorescence apparatus and immunoassay WO1988007670A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US031,408 1987-03-27
US031,288 1987-03-27
US07/031,408 US4923819A (en) 1987-03-27 1987-03-27 Time-resolved fluorescence immunoassay
US07/031,288 US4855930A (en) 1987-03-27 1987-03-27 Method and appartatus for improved time-resolved fluorescence spectroscopy

Publications (2)

Publication Number Publication Date
WO1988007670A2 WO1988007670A2 (en) 1988-10-06
WO1988007670A3 true WO1988007670A3 (en) 1989-01-26

Family

ID=26707047

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/001019 WO1988007670A2 (en) 1987-03-27 1988-03-24 Time-resolved fluorescence apparatus and immunoassay

Country Status (2)

Country Link
ES (1) ES2006611A6 (en)
WO (1) WO1988007670A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2224352B (en) * 1988-10-05 1992-09-16 Hamamatsu Photonics Kk Light waveform measuring apparatus
FI82144C (en) * 1989-03-22 1991-01-10 Wallac Oy FOERFARANDE FOER SAMTIDIG BESTAEMNING AV FLERA LIGANDER.
US4977325A (en) * 1989-07-12 1990-12-11 P B Diagnostic Systems, Inc. Optical read system and immunoassay method
CA2026257C (en) * 1989-09-26 2001-07-24 Keiji Sasaki Device for analyzing a transient waveform
DE19718016A1 (en) * 1997-04-29 1998-11-05 Drexhage Karl Heinz Method for the optical detection of analyte molecules in a natural biological medium
GB0001089D0 (en) 2000-01-18 2000-03-08 Council Cent Lab Res Councils Lipoprotein assay
DE10308814A1 (en) * 2003-02-27 2004-09-09 Chromeon Gmbh Bioanalytical method based on the measurement of the decay time of phosphorescence
CA2806306A1 (en) * 2010-07-23 2012-01-26 Luminex Corporation Co-coupling to control reactivity of reagents in immunoassays
US9062342B2 (en) 2012-03-16 2015-06-23 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
CN103558433B (en) * 2013-10-24 2016-03-30 北京无线电计量测试研究所 A kind of device and method measured for photodetector impulse response
CN103728446B (en) * 2013-12-11 2016-02-24 天津大学 Photon counting-type hyperchannel time resolved fluoro-immunoassay system and method for counting

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4368047A (en) * 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
GB2129548A (en) * 1982-11-01 1984-05-16 Hamamatsu Corp Compact temporal spectral photometer
WO1986002734A1 (en) * 1984-10-31 1986-05-09 Hyperion Catalysis International, Inc. Luminescent metal chelate labels and means for detection
JPH06128844A (en) * 1992-10-20 1994-05-10 Tsudakoma Corp Separator for defective weft yarn in multicolor rapier loom

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
US4368047A (en) * 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
GB2129548A (en) * 1982-11-01 1984-05-16 Hamamatsu Corp Compact temporal spectral photometer
WO1986002734A1 (en) * 1984-10-31 1986-05-09 Hyperion Catalysis International, Inc. Luminescent metal chelate labels and means for detection
JPH06128844A (en) * 1992-10-20 1994-05-10 Tsudakoma Corp Separator for defective weft yarn in multicolor rapier loom

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
1969 Wescon Technical Papers, Part 4 Instruments and Systems, Western Electronic Show and Convention, 19-22 August, 1969 (US) G.T. Longerbeam et al.: "High speed single transient oscilloscopes, the state of the art, and current potential for mating to on-line computers", pages 13/4: 1-10 *
Japanese Journal of Applied Physics, vol. 15, no. 5, 1976 (Tokyo, JP)H. Saito et al.: "A high sensitivity television camera system for pico-second spectroscopy", pages 947,948 *
Modern Fluorescence Spectroscopy, vol. 4, 1981, Plenum Press (New York, US) J.H. Richardson: "Applications of lasers in analytical molecular fluorescence spectroscopy", page 15 *
Patent Abstracts of Japan, vol. 10, no. 180 (P-471)(2236) 24 June 1986; & JP-A-6128844 (HAMAMATSU PHOTONICS K.K.) 8 February 1986 *
R.I. Sha'afi et al.: "Fast methods in physical biochemistry and cell biology", published 1983 by Elsevier (Amsterdam, NL) S.M. Fernandez: "Time-resolved fluorescence spectroscopy", pages 221-279 *
Review of Scientific Instruments, vol. 52, no. 11, November 1981 (New York, US) F. Docchio et al.: "Subnanosecond fluorescence waveforms measurements with a dual time-scale microprocessor-controlled averager", pages 1671-1675 *

Also Published As

Publication number Publication date
WO1988007670A2 (en) 1988-10-06
ES2006611A6 (en) 1989-05-01

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