WO1990012111A1 - Improved primer extension reactions - Google Patents
Improved primer extension reactions Download PDFInfo
- Publication number
- WO1990012111A1 WO1990012111A1 PCT/US1990/001938 US9001938W WO9012111A1 WO 1990012111 A1 WO1990012111 A1 WO 1990012111A1 US 9001938 W US9001938 W US 9001938W WO 9012111 A1 WO9012111 A1 WO 9012111A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- dna polymerase
- agent
- extension
- pyrophosphate
- stranded
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002050276A CA2050276C (en) | 1989-04-12 | 1990-04-10 | Improved primer extension reactions |
KR1019900702622A KR920700294A (en) | 1989-04-12 | 1990-04-10 | Improved Primer Extension |
NO91914013A NO914013L (en) | 1989-04-12 | 1991-10-11 | IMPROVED PRIMARY EXTENSION REACTIONS |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33675189A | 1989-04-12 | 1989-04-12 | |
US336,751 | 1989-04-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1990012111A1 true WO1990012111A1 (en) | 1990-10-18 |
Family
ID=23317491
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1990/001938 WO1990012111A1 (en) | 1989-04-12 | 1990-04-10 | Improved primer extension reactions |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0467953A4 (en) |
JP (1) | JP2997043B2 (en) |
KR (1) | KR920700294A (en) |
AU (1) | AU638246B2 (en) |
CA (1) | CA2050276C (en) |
HU (1) | HUT61054A (en) |
LT (1) | LTIP1519A (en) |
WO (1) | WO1990012111A1 (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2674254A1 (en) * | 1991-03-20 | 1992-09-25 | Univ Reims Champagne Ardenne | NON-RADIOACTIVE DETECTION OF THE PRESENCE OF A DETERMINED NUCLEIC ACID IN A BIOLOGICAL SAMPLE. |
EP0527728A1 (en) * | 1989-03-24 | 1993-02-24 | Consejo Superior Investigacion | -i(IN VITRO) DNA SYNTHESIS REACTIONS USING PHI 29 DNA POLYMERASE AND A DNA FRAGMENT ENCODING SAID POLYMERASE. |
US5256555A (en) * | 1991-12-20 | 1993-10-26 | Ambion, Inc. | Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions |
WO1994005797A1 (en) * | 1992-09-01 | 1994-03-17 | Vsevolod Kiselev | Synthesis of dna molecules in vitro |
EP0649909A2 (en) * | 1993-10-23 | 1995-04-26 | Roche Diagnostics GmbH | Stabilised liquid mixtures for the labelling of nucleic acids |
US5576204A (en) * | 1989-03-24 | 1996-11-19 | Consejo Superior Investigaciones Cientificas | φ29 DNA polymerase |
EP0745676A1 (en) * | 1995-05-31 | 1996-12-04 | Amersham Life Science Inc | Thermostable DNA polymerases |
US5665551A (en) * | 1995-09-13 | 1997-09-09 | Roche Molecular Systems, Inc. | Purified nucleic acid encoding a thermostable pyrophosphatase |
WO1997037038A1 (en) * | 1996-03-29 | 1997-10-09 | Boehringer Mannheim Gmbh | Process for the specific multiplication of long nucleic acids by pcr |
WO1998015655A1 (en) * | 1996-10-07 | 1998-04-16 | The Perkin-Elmer Corporation | Primer extension reaction utilizing a cosubstrate-enzyme pair for consuming pyrophosphate |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
GB2377991A (en) * | 2001-04-30 | 2003-01-29 | Secr Defence Brit | DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase |
EP1704246A2 (en) * | 2003-09-30 | 2006-09-27 | Perkinelmer Las, Inc. | Compositions and processes for genotyping single nucleotide polymorphisms |
WO2014187924A1 (en) * | 2013-05-24 | 2014-11-27 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
WO2022155282A1 (en) * | 2021-01-14 | 2022-07-21 | Integrated Dna Technologies, Inc. | Methods for production and quantification of unique molecular identifier-labeled beads |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
US4971903A (en) * | 1988-03-25 | 1990-11-20 | Edward Hyman | Pyrophosphate-based method and apparatus for sequencing nucleic acids |
-
1990
- 1990-04-10 WO PCT/US1990/001938 patent/WO1990012111A1/en not_active Application Discontinuation
- 1990-04-10 EP EP19900906516 patent/EP0467953A4/en not_active Withdrawn
- 1990-04-10 HU HU903562A patent/HUT61054A/en unknown
- 1990-04-10 AU AU54382/90A patent/AU638246B2/en not_active Ceased
- 1990-04-10 JP JP2506210A patent/JP2997043B2/en not_active Expired - Lifetime
- 1990-04-10 CA CA002050276A patent/CA2050276C/en not_active Expired - Lifetime
- 1990-04-10 KR KR1019900702622A patent/KR920700294A/en not_active Application Discontinuation
-
1993
- 1993-12-03 LT LTIP1519A patent/LTIP1519A/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683202B1 (en) * | 1985-03-28 | 1990-11-27 | Cetus Corp |
Non-Patent Citations (1)
Title |
---|
See also references of EP0467953A4 * |
Cited By (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0527728A1 (en) * | 1989-03-24 | 1993-02-24 | Consejo Superior Investigacion | -i(IN VITRO) DNA SYNTHESIS REACTIONS USING PHI 29 DNA POLYMERASE AND A DNA FRAGMENT ENCODING SAID POLYMERASE. |
EP0527728A4 (en) * | 1989-03-24 | 1994-11-23 | Consejo Superior Investigacion | -i(in vitro) dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase |
US5576204A (en) * | 1989-03-24 | 1996-11-19 | Consejo Superior Investigaciones Cientificas | φ29 DNA polymerase |
WO1992016654A1 (en) * | 1991-03-20 | 1992-10-01 | Universite De Reims Champagne-Ardenne | Non-radioactive determination of the presence of a given nucleic acid in a biological sample |
FR2674254A1 (en) * | 1991-03-20 | 1992-09-25 | Univ Reims Champagne Ardenne | NON-RADIOACTIVE DETECTION OF THE PRESENCE OF A DETERMINED NUCLEIC ACID IN A BIOLOGICAL SAMPLE. |
US5256555A (en) * | 1991-12-20 | 1993-10-26 | Ambion, Inc. | Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions |
US6586218B2 (en) | 1991-12-20 | 2003-07-01 | Ambion, Inc. | Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions |
US6586219B2 (en) | 1991-12-20 | 2003-07-01 | Ambion, Inc. | Compositions and methods for increasing the yields of the in vitro RNA transcription and other polynucleotide synthetic reactions |
WO1994005797A1 (en) * | 1992-09-01 | 1994-03-17 | Vsevolod Kiselev | Synthesis of dna molecules in vitro |
EP0649909A3 (en) * | 1993-10-23 | 1999-03-31 | Roche Diagnostics GmbH | Stabilised liquid mixtures for the labelling of nucleic acids |
EP0649909A2 (en) * | 1993-10-23 | 1995-04-26 | Roche Diagnostics GmbH | Stabilised liquid mixtures for the labelling of nucleic acids |
EP0745676A1 (en) * | 1995-05-31 | 1996-12-04 | Amersham Life Science Inc | Thermostable DNA polymerases |
US5885813A (en) * | 1995-05-31 | 1999-03-23 | Amersham Life Science, Inc. | Thermostable DNA polymerases |
WO1996038568A1 (en) * | 1995-05-31 | 1996-12-05 | Amersham Life Science, Inc. | Thermostable dna polymerases |
US5665551A (en) * | 1995-09-13 | 1997-09-09 | Roche Molecular Systems, Inc. | Purified nucleic acid encoding a thermostable pyrophosphatase |
WO1997037038A1 (en) * | 1996-03-29 | 1997-10-09 | Boehringer Mannheim Gmbh | Process for the specific multiplication of long nucleic acids by pcr |
WO1998015655A1 (en) * | 1996-10-07 | 1998-04-16 | The Perkin-Elmer Corporation | Primer extension reaction utilizing a cosubstrate-enzyme pair for consuming pyrophosphate |
US7344835B2 (en) | 1996-11-22 | 2008-03-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6764839B2 (en) | 1996-11-22 | 2004-07-20 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6951744B2 (en) | 2001-04-30 | 2005-10-04 | The Secretary Of State For Defence | Amplification process |
GB2377991A (en) * | 2001-04-30 | 2003-01-29 | Secr Defence Brit | DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase |
GB2377991B (en) * | 2001-04-30 | 2004-01-28 | Secr Defence Brit | DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase |
US7449312B2 (en) | 2001-04-30 | 2008-11-11 | The Secretary Of State For Defense In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Kit for conducting a polymerase chain reaction |
EP1704246A2 (en) * | 2003-09-30 | 2006-09-27 | Perkinelmer Las, Inc. | Compositions and processes for genotyping single nucleotide polymorphisms |
EP1704246A4 (en) * | 2003-09-30 | 2007-11-28 | Perkinelmer Las Inc | Compositions and processes for genotyping single nucleotide polymorphisms |
WO2014187924A1 (en) * | 2013-05-24 | 2014-11-27 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
EP3351645A1 (en) * | 2013-05-24 | 2018-07-25 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing using nanopores |
US10450605B2 (en) | 2013-05-24 | 2019-10-22 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
EP3825415A1 (en) * | 2013-05-24 | 2021-05-26 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing using nanopores |
US11286521B2 (en) | 2013-05-24 | 2022-03-29 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
WO2022155282A1 (en) * | 2021-01-14 | 2022-07-21 | Integrated Dna Technologies, Inc. | Methods for production and quantification of unique molecular identifier-labeled beads |
Also Published As
Publication number | Publication date |
---|---|
CA2050276C (en) | 2003-03-11 |
HUT61054A (en) | 1992-11-30 |
EP0467953A1 (en) | 1992-01-29 |
KR920700294A (en) | 1992-02-19 |
JPH04506002A (en) | 1992-10-22 |
LTIP1519A (en) | 1995-06-26 |
HU903562D0 (en) | 1992-03-30 |
JP2997043B2 (en) | 2000-01-11 |
AU638246B2 (en) | 1993-06-24 |
AU5438290A (en) | 1990-11-05 |
CA2050276A1 (en) | 1990-10-13 |
EP0467953A4 (en) | 1992-06-17 |
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