WO1991013136A1 - Process for treatment of textiles and rinsing agent for use in the process - Google Patents
Process for treatment of textiles and rinsing agent for use in the process Download PDFInfo
- Publication number
- WO1991013136A1 WO1991013136A1 PCT/DK1991/000057 DK9100057W WO9113136A1 WO 1991013136 A1 WO1991013136 A1 WO 1991013136A1 DK 9100057 W DK9100057 W DK 9100057W WO 9113136 A1 WO9113136 A1 WO 9113136A1
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- WIPO (PCT)
- Prior art keywords
- enzyme
- agent
- rinsing
- process according
- fact
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
Definitions
- the invention relates to a process for treatment of textiles by use of an enzyme and to a rinsing agent for use therein.
- Treatment of soiled or unsoiled textiles normally comprises washing with a detergent containing anionic and/or nonionic surfactant, followed by rinsing and drying.
- a detergent containing anionic and/or nonionic surfactant can be incorporated into the detergent to improve the removal of fat soiling (EP 130,064, EP 214,761 , US 4,810,414).
- a non-dusting granulate containing a cellulase can be added to a main wash detergent composition in order to reduce the harshness of the laundry (US 4,435,307).
- proteases are useful in detergent compositions in order to remove proteinaceous stains (US 3,723,250), and that amylases are useful in detergent compositions in order to remove starchy stains (US 3,627,688 and 4,548,727).
- a given enzyme may be incompatible with a given detergent composition due to unsatisfactory activity and stability of the enzyme, and this imposes some limitations in the choice of enzyme and detergent formulation. It is the object of the invention to provide an alternative method of using enzyme in textile treatment, so as to obtain efficient enzyme effects for a given enzyme dosage while avoiding the above limitations.
- an enzyme provides a better enzymatic effect when used in the rinse liquor than when used as a constituent of the main wash composition.
- the effect is particularly pronounced after repeated cycles of washing, rinsing and drying.
- Detergent is essentially absent during rinsing, and thus compatibility problems are avoided.
- the enzyme may advantageously be added in the last rinse step together with a fabric softening and/or antistatic agent.
- the invention provides a process for treatment of textiles by use of an enzyme, characterized by the fact that the enzyme is used in a rinse liquor for a rinsing step after washing.
- the enzyme is used in the rinse liquor for the rinsing step after washing together with a fabric softening agent and/or an antistatic agent.
- a fabric softening agent and/or an antistatic agent An even better enzymatic effect is observed in this embodiment, demonstrating a synergism between enzyme and fabric softening agent and/or antistatic agent.
- the fabric softening agent and/or the antistatic agent is a cationic surfactant.
- a satisfactory enzymatic effect is observed in this embodiment.
- the enzyme is used in the rinse liquor for a second or later rinsing step, preferably in the last rinsing step. In this manner an even better enzymatic effect is observed.
- the enzyme is a lipase and/or a cellulase and/or a protease and/or an amylase. These are the four most commonly used detergent enzymes.
- the enzyme is a lipase and/or a cellulase. In relation to these two enzymes the best improvement according to the invention has been demonstrated. In a preferred embodiment of the process according to the invention the enzyme(s) is (are) of microbial origin. In this manner cheap enzymes can be provided.
- the enzyme is cellulase
- the softening agent and the antistatic agent is a cationic surfactant
- the rinse liquor contains additionally a non-ionic surfactant.
- the concentration of enzyme in the rinse liquor is in the range of 0.001 to 5, preferably 0.01 to 2 mg pure enzyme protein per liter of rinse liquor. At a concentration below 0.001 mg pure enzyme protein per liter of rinse liquor no significant enzyme effect can be observed, and at a concentration above 5 mg pure enzyme protein per liter of rinse liquor the enzyme cost will be unreasonably high.
- the invention comprises a rinsing agent for use in the process according to the invention, this rinsing agent being characterized by the fact that it is an enzyme preparation stabilized against microbial infection.
- the rinsing agent also comprises a fabric softening agent and/or an antistatic agent. In this manner the enzymatic effect during the rinsing process is improved.
- the rinsing agent is aqueous and it exhibits an enzyme activity between 0.01 and 50 mg pure enzyme protein per liter of rinsing agent, preferably between 0.5 and 10 mg pure enzyme protein per liter of rinsing agent, and the content of fabric softening and/or antistatic agent is at least 1% (by weight) (% as active material). It is intended that this rinsing agent is added to the rinse liquor, both providing the proper concentration of enzyme and the proper amount of fabric softening agent and/or antistatic agent.
- the enzyme is cellulase
- the softening agent and the antistatic agent is a cationic surfactant
- the rinsing agent contains additionally a non-ionic surfactant.
- Lipases of plant or animal origin can be used in the invention, but microbial lipases are preferred for reasons of economy.
- Lipases already known to be active in detergents can be used in the invention, but since the conditions in the rinsing step are favourable for most lipases, many other lipases can also be used.
- lipases derived from the following microorganisms.
- the indicated patent publications are incorporated herein by reference: Humicola, e.g. H. insolens (US 4,810,414)
- Pseudomonas e.g. Ps. cepacia or Ps. fluorescens (WO 89/04361). Fusarium, e.g. F. oxysporum (EP 130,064). Mucor (also called Rhizomuco ⁇ , e.g. M. miehei. Candida, e.g. C. cylindracea (also called C. rugosa) or C. antarctica (WO 88/02775).
- the lipase activity unit, LU is defined in AF 95/5, which is available on request from Novo Nordisk A/S, Novo alle, DK-2880 Bagsvaerd, Denmark.
- Cellulases may be derived from Humicola insolens, proteases from Bacillus licheniformis, and amylases may be derived from Bacillus subtilis.
- CEVU cellulase activity unit
- Fabric softening agents and/or antistatic agents are provided.
- a fabric softening and/or antistatic agent effective for fabric after-treatment is used together with the enzyme in the process according to the invention.
- Such after-treatment agents are known in the art.
- One example is quaternary ammonium salts such as dialkyl (Cig-C- j ) dimethyl ammonium chloride, alkyl (C32-C 3 g) trimethyl ammonium chloride or alkyl (Cg-C- j g) dimethyl benzyl ammonium chloride (ABDAC); and alkyl imidazolinium methyl sulphate.
- Another example is layered clay of the smectite type, such as montmorillonite or bentonite.
- the invention uses an enzyme in the rinsing stage of a conventional treatment process for soiled or unsoiled fabrics comprising washing, rinsing and drying.
- the rinsing stage generally comprises 2-5 subsequent steps of rinsing with water, usually 3 or 4 steps.
- the enzyme is added together with a fabric softening and/or antistatic agent (e.g. 0.1-0.5 g/l).
- the enzyme is preferably added in the second or a later rinsing step, and most pre ⁇ ferably in the last rinsing step, where the detergent concentration is lowest (thus minimizing any incompatibility problems between detergent and enzyme) and where it can conveniently be added together with the fabric softening agent and/or antistatic agent, if such agent is used in the process according to the invention.
- Conventional rinsing conditions can be used, e.g. 10-30 ⁇ C water, 1-20 minutes at near-neutral pH (e.g. 5-9) depending on the use of cationic or clay.
- the rinsing agent according to the invention is stabilized against microbial infection, preferably by incorporation of a stabilizing agent known in the art.
- a stabilizing agent known in the art.
- examples are inorganic salts (such as NaCI), sugars (such as sucrose and glucose), polyols (such as glycerol, propylene glycol and sorbitol) and alcohols (such as ethanol and iso-propanol). These are generally effective in amounts above 10%, especially above 20%.
- organic acids such as benzoic, sorbic, propionic, lactic and formic
- stabilizing agents are antioxidants (such as sulphur dioxide), 1 ,2-benz-iso-thiazolin-3-one (BIT) and parabens. Some of these may also serve to improve enzyme stability.
- Further additives may be incorporated to improve enzyme stability or physical stability of the rinsing agent.
- the choice depends on the type of enzyme(s). Examples are CaCl2 (e.g. 0.1-0.5%), a protease inhibitor (such as borate and formate) or 0.5-5% of lower alcohol (such as ethanol or iso-propanol, preferably used together with a larger amount of a polyol).
- the rinsing agent ordinarily is preferably essentially devoid of anionic and nonionic surfactant (total amount of these below 5%, most preferably below 2%).
- the rinsing agent may be in solid (e.g. as non-dusting granules) or liquid form (e.g. aqueous). It may be formulated by incorporating enzyme into known softening/antistatic compositions.
- the content of the softening/antistatic agent is preferably 1-50%, especially 5-50% (% as active material).
- the lipase used in Examples 1 - 4 is from Humicola lanuginosa produced according to US 4,810,414 or from Pseudomonas cepacia produced according to WO 89/04361.
- the cellulase used in Example 5 is from Humicola insolens produced according to US 4,435,307. EXAMPLE 1
- Soiling Lard/Sudan red (1000/0.75 w/w) is heated to 70 ⁇ C, and 50 ⁇ is spotted onto each swatch by a pipette. After application of stain, the swatches are heated in an oven for 30 minutes at 75 °C. Washing: Terg-o-tometer (100 rpm). Commercial European detergent powder, 8 g/l in 18°dH water. pH not adjusted (approx. 10). 30° C, 20 minutes.
- 4th rinse 10 minutes in 1 I tap water (22° C). Swatches squeezed by hand between each rinse. 0 or 100 LU/I of lipase added in 4th rinse. 0 or 0.20 g/l of Arquad 2HT-75 (dialkyl dimethyl ammonium chloride, 75% active matter, Akzo-Chemicals) added in 4th rinse.
- Arquad 2HT-75 dialkyl dimethyl ammonium chloride, 75% active matter, Akzo-Chemicals
- Reflectance is measured at 460 nm. Content of fatty matter is determined by Soxhlet extraction with chloroform for 5 hours. It appears from the following tables that addition of lipase during the last rinse improves the removal of fatty matter, in relation to both lipases, both with and without cationic.
- the treatment consists of a washing process under US conditions followed by a 4 repeated rinsings.
- the enzyme is introduced respectively in the main wash liquor (prior art) and in the rinse liquor corresponding to the last rinsing step (the invention).
- the cellulase is produced by means of Humicola insolens DSM 1800, and the CEVU cellulase activity unit is defined in AF 253/2-GB.
- Nonionic Berol 081, 100% AEO C 16 -C 18 , 30 mole EO, Berol Kemi AB,
- the surface of the swatches is analysed by measuring reflected light.
- the effect of the cellulase is removal of damaged cellulose fibres causing a grey/worn look, and by measuring the grey scale value a quantitative expression for the enzyme treatment is obtained.
- the results are represented in relation to the L-coordinate of a surface not treated with cellulase:
- composition 1 2 3 4
Abstract
In the process for treatment of textiles by use of an enzyme the enzyme is used in a rinse liquor for a rinsing step after washing. The rinsing agent is an enzyme preparation stabilized against microbial infection. Use of the enzyme in the process provides a better enzymatic effect when used in the rinse liquor than when used as a constituent of the main wash composition.
Description
PROCESS FOR TREATMENT OF TEXTILES AND RINSING AGENT FOR USE IN THE PROCESS
TECHNICAL FIELD
The invention relates to a process for treatment of textiles by use of an enzyme and to a rinsing agent for use therein.
BACKGROUND ART
Treatment of soiled or unsoiled textiles normally comprises washing with a detergent containing anionic and/or nonionic surfactant, followed by rinsing and drying. It belongs to the prior art that lipase can be incorporated into the detergent to improve the removal of fat soiling (EP 130,064, EP 214,761 , US 4,810,414). Also, it belongs to the prior art that a non-dusting granulate containing a cellulase can be added to a main wash detergent composition in order to reduce the harshness of the laundry (US 4,435,307). Furthermore, it belongs to the prior art that proteases are useful in detergent compositions in order to remove proteinaceous stains (US 3,723,250), and that amylases are useful in detergent compositions in order to remove starchy stains (US 3,627,688 and 4,548,727).
However, a given enzyme may be incompatible with a given detergent composition due to unsatisfactory activity and stability of the enzyme, and this imposes some limitations in the choice of enzyme and detergent formulation. It is the object of the invention to provide an alternative method of using enzyme in textile treatment, so as to obtain efficient enzyme effects for a given enzyme dosage while avoiding the above limitations.
STATEMENT OF THE INVENTION
We have found that, surprisingly, use of an enzyme provides a better enzymatic effect when used in the rinse liquor than when used as a constituent of the main wash composition. The effect is particularly pronounced after repeated cycles of washing, rinsing and drying. Detergent is essentially absent during rinsing, and thus compatibility problems are avoided. The enzyme may advantageously be added in the last rinse step together with a fabric softening and/or antistatic agent.
Accordingly, the invention provides a process for treatment of textiles by use of an enzyme, characterized by the fact that the enzyme is used in a rinse liquor for a rinsing step after washing.
In a preferred embodiment of the process according to the invention the enzyme is used in the rinse liquor for the rinsing step after washing together with a fabric softening agent and/or an antistatic agent. An even better enzymatic effect is observed in this embodiment, demonstrating a synergism between enzyme and fabric softening agent and/or antistatic agent.
In a preferred embodiment of the process according to the invention the fabric softening agent and/or the antistatic agent is a cationic surfactant. A satisfactory enzymatic effect is observed in this embodiment. In a preferred embodiment of the process according to the invention the enzyme is used in the rinse liquor for a second or later rinsing step, preferably in the last rinsing step. In this manner an even better enzymatic effect is observed.
In a preferred embodiment of the process according to the invention the enzyme is a lipase and/or a cellulase and/or a protease and/or an amylase. These are the four most commonly used detergent enzymes.
In a preferred embodiment of the process according to the invention the enzyme is a lipase and/or a cellulase. In relation to these two enzymes the best improvement according to the invention has been demonstrated.
In a preferred embodiment of the process according to the invention the enzyme(s) is (are) of microbial origin. In this manner cheap enzymes can be provided.
In a preferred embodiment of the process according to the invention the enzyme is cellulase, the softening agent and the antistatic agent is a cationic surfactant, and the rinse liquor contains additionally a non-ionic surfactant. A very marked improvement according to the invention has been demonstrated under these conditions.
In a preferred embodiment of the process according to the invention the concentration of enzyme in the rinse liquor is in the range of 0.001 to 5, preferably 0.01 to 2 mg pure enzyme protein per liter of rinse liquor. At a concentration below 0.001 mg pure enzyme protein per liter of rinse liquor no significant enzyme effect can be observed, and at a concentration above 5 mg pure enzyme protein per liter of rinse liquor the enzyme cost will be unreasonably high.
Also, the invention comprises a rinsing agent for use in the process according to the invention, this rinsing agent being characterized by the fact that it is an enzyme preparation stabilized against microbial infection.
In a preferred embodiment of the rinsing agent according to the invention the rinsing agent also comprises a fabric softening agent and/or an antistatic agent. In this manner the enzymatic effect during the rinsing process is improved.
In a preferred embodiment of the rinsing agent according to the invention the rinsing agent is aqueous and it exhibits an enzyme activity between 0.01 and 50 mg pure enzyme protein per liter of rinsing agent, preferably between 0.5 and 10 mg pure enzyme protein per liter of rinsing agent, and the content of fabric softening and/or antistatic agent is at least 1% (by weight) (% as active material). It is intended that this rinsing agent is added to the rinse liquor, both providing the proper concentration of enzyme and the proper amount of fabric softening agent and/or antistatic agent.
In a preferred embodiment of the rinsing agent according to the invention the enzyme is cellulase, the softening agent and the antistatic agent is a cationic surfactant, and the rinsing agent contains additionally a non-ionic surfactant. In this embodiment a very marked improvement according to the invention has been demonstrated.
DETAILED DESCRIPTION OF THE INVENTION
Lipases
Lipases of plant or animal origin (e.g. pancreas lipase) can be used in the invention, but microbial lipases are preferred for reasons of economy. Lipases already known to be active in detergents can be used in the invention, but since the conditions in the rinsing step are favourable for most lipases, many other lipases can also be used.
Examples are lipases derived from the following microorganisms. The indicated patent publications are incorporated herein by reference: Humicola, e.g. H. insolens (US 4,810,414)
Pseudomonas, e.g. Ps. cepacia or Ps. fluorescens (WO 89/04361). Fusarium, e.g. F. oxysporum (EP 130,064). Mucor (also called Rhizomucoή, e.g. M. miehei. Candida, e.g. C. cylindracea (also called C. rugosa) or C. antarctica (WO 88/02775).
The lipase activity unit, LU, is defined in AF 95/5, which is available on request from Novo Nordisk A/S, Novo alle, DK-2880 Bagsvaerd, Denmark.
Cellulases, proteases, and amylases
Similar considerations as indicated in the preceding paragraph about lipases are valid for the cellulases, proteases, and amylases. Cellulases may be
derived from Humicola insolens, proteases from Bacillus licheniformis, and amylases may be derived from Bacillus subtilis.
The cellulase activity unit, CEVU, is defined in AF 253/2-GB, which is available on request from Novo Nordisk A/S, Novo alle, DK-2880 Bagsvaerd, Denmark.
Fabric softening agents and/or antistatic agents
According to a preferred embodiment of the invention a fabric softening and/or antistatic agent effective for fabric after-treatment is used together with the enzyme in the process according to the invention. Such after-treatment agents are known in the art. One example is quaternary ammonium salts such as dialkyl (Cig-C-j ) dimethyl ammonium chloride, alkyl (C32-C3g) trimethyl ammonium chloride or alkyl (Cg-C-jg) dimethyl benzyl ammonium chloride (ABDAC); and alkyl imidazolinium methyl sulphate. Another example is layered clay of the smectite type, such as montmorillonite or bentonite.
Textile treatment process
The invention uses an enzyme in the rinsing stage of a conventional treatment process for soiled or unsoiled fabrics comprising washing, rinsing and drying. The rinsing stage generally comprises 2-5 subsequent steps of rinsing with water, usually 3 or 4 steps. In a preferred embodiment the enzyme is added together with a fabric softening and/or antistatic agent (e.g. 0.1-0.5 g/l). The enzyme is preferably added in the second or a later rinsing step, and most pre¬ ferably in the last rinsing step, where the detergent concentration is lowest (thus minimizing any incompatibility problems between detergent and enzyme) and where it can conveniently be added together with the fabric softening agent and/or antistatic agent, if such agent is used in the process according to the invention. Conventional rinsing conditions can be used, e.g. 10-30βC water, 1-20 minutes at near-neutral pH (e.g. 5-9) depending on the use of cationic or clay.
Rinsing agent
The rinsing agent according to the invention is stabilized against microbial infection, preferably by incorporation of a stabilizing agent known in the art. Examples are inorganic salts (such as NaCI), sugars (such as sucrose and glucose), polyols (such as glycerol, propylene glycol and sorbitol) and alcohols (such as ethanol and iso-propanol). These are generally effective in amounts above 10%, especially above 20%. Another example is organic acids (such as benzoic, sorbic, propionic, lactic and formic), which are generally effective in amounts of 0.01-2% at low pH (below 5). Other examples of stabilizing agents are antioxidants (such as sulphur dioxide), 1 ,2-benz-iso-thiazolin-3-one (BIT) and parabens. Some of these may also serve to improve enzyme stability.
Further additives may be incorporated to improve enzyme stability or physical stability of the rinsing agent. The choice depends on the type of enzyme(s). Examples are CaCl2 (e.g. 0.1-0.5%), a protease inhibitor (such as borate and formate) or 0.5-5% of lower alcohol (such as ethanol or iso-propanol, preferably used together with a larger amount of a polyol).
The rinsing agent ordinarily is preferably essentially devoid of anionic and nonionic surfactant (total amount of these below 5%, most preferably below 2%). The rinsing agent may be in solid (e.g. as non-dusting granules) or liquid form (e.g. aqueous). It may be formulated by incorporating enzyme into known softening/antistatic compositions. The content of the softening/antistatic agent is preferably 1-50%, especially 5-50% (% as active material).
EXAMPLES
The lipase used in Examples 1 - 4 is from Humicola lanuginosa produced according to US 4,810,414 or from Pseudomonas cepacia produced according to WO 89/04361. The cellulase used in Example 5 is from Humicola insolens produced according to US 4,435,307.
EXAMPLE 1
Effect of different lipases under the rinse process
Washing and rinsing tests are made as follows:
Fabric: Polyester/cotton (50/50) or cotton. Swatch size: 9x9 cm.
Soiling: Lard/Sudan red (1000/0.75 w/w) is heated to 70βC, and 50 μ\ is spotted onto each swatch by a pipette. After application of stain, the swatches are heated in an oven for 30 minutes at 75 °C. Washing: Terg-o-tometer (100 rpm). Commercial European detergent powder, 8 g/l in 18°dH water. pH not adjusted (approx. 10). 30° C, 20 minutes.
7 swatches per beaker containing 1 liter detergent solution. Rinsing: 4-step rinse in Terg-o-tometer. 1st-3rd rinse: 6 minutes in 1 I cold tap water.
4th rinse: 10 minutes in 1 I tap water (22° C). Swatches squeezed by hand between each rinse. 0 or 100 LU/I of lipase added in 4th rinse. 0 or 0.20 g/l of Arquad 2HT-75 (dialkyl dimethyl ammonium chloride, 75% active matter, Akzo-Chemicals) added in 4th rinse.
Drying: Line-drying (16 hours) at room temperature.
Repeats: Washing, rinsing and drying of swatches repeated 3 times.
Evaluation: After the 3rd wash.
Reflectance is measured at 460 nm. Content of fatty matter is determined by Soxhlet extraction with chloroform for 5 hours.
It appears from the following tables that addition of lipase during the last rinse improves the removal of fatty matter, in relation to both lipases, both with and without cationic.
Fatty matter
Arquad
Lipase 2HT-75 Reflectance mg Residual
(g/i) R ΔR fat %
Effect of Humicola lanuginosa and of Pseudomonas cepacia lipase on lard/cotton swatches. The lipases were added initally under the 4th rinse step; no lipase was present during the washing process.
Arquad
Lipase 2HT-75
Effect of Humicola lanuginosa and of Pseudomonas cepacia lipase on lard/polyester-cotton swatches. The lipases were added initially under the 4th rinse step; no lipase was present during the washing process.
EXAMPLE 2
Lipase performance when used in combination with different cationic surfactants
The performance evaluation was carried out as described in Example
1 , the only difference being that other kinds of cationics were used in combination with the Humicola lanuginosa lipase and that the lipase dosage was varied between 0 and 3000 LU per litre. The cationics used were:
1) Dodigen 1490 from Hoechst (dialkyl dimethyl ammonium chloride, 75% active matter)
2) Empigen FRC75/S from Albright & Wilson (alkyl imidazoline methosulfate, 75% active matter)
EXAMPLE 3
Lipase performance - comparison with prior art
Performance evaluation was carried out as described in Example 1. In this example the use of Humicola lanuginosa lipase in the rinse liquor (this invention) was compared to the use of the same enzyme in the main wash liquor (prior art). Lipase dosage was 100 LU/I.
EXAMPLE 4
Lipase stability in rinse preparations (after treatment preparations)
Composition % w/w
The above mentioned lipase containing rinse preparations were stored at room temperature (20-22° C) for 240 days and the lipase activity was followed. The following results expressed as % relative activity were obtained:
EXAMPLE 5
Effect of cellulase on color clarification The textile swatches are exposed to the enzyme in a Terg-o-tometer.
The treatment consists of a washing process under US conditions followed by a 4 repeated rinsings. The enzyme is introduced respectively in the main wash liquor (prior art) and in the rinse liquor corresponding to the last rinsing step (the invention).
Wash:
Liquid volume 800 ml
Agitation 100 movements/minute
Washing time 10 minutes
Washing 20° C pH 7.0
Water hardness 9 dH
Textile 100% cotton
Amount of textile 2 swatches with dimensions 10x15 cm in 800 ml of liquor Detergent : 5 g/l commercial US detergent based on anionics
Rinsing steps:
Liquid volume 800 ml
Agitation 100 movements/minute
Rinsing time, step 1-3 6 minutes
Rinsing time, step 4 10 minutes
Rinsing temperature 20° C pH 7.0
Water hardness 9 dH
Drying The swatches are dried at room temperature after the last step
Cellulase dosages:
0 - 500 CEVU/I. The cellulase is produced by means of Humicola insolens DSM 1800, and the CEVU cellulase activity unit is defined in AF 253/2-GB.
Rinse liguor compositions:
Cationic: ARQUAD 2HT-75, dialkyl dimethyl ammonium chloride, Akzo
Chemie GmbH.
Nonionic: Berol 081, 100% AEO C16-C18, 30 mole EO, Berol Kemi AB,
Sweden.
Enzyme introduction the 4th rinsing step the 4th rinsing step the 4th rinsing step
in wash
Analysis method:
The surface of the swatches is analysed by measuring reflected light.
White light is projected onto the surface and the reflection/remission is detected at 16 wavelengths by means of the "Elrepho 2000", Datacolor Schweiz apparatus. The results from these detections are processed into Hunter coordinates of which the L-coordinate represents the grey scale value (100: black, 0: white).
The effect of the cellulase is removal of damaged cellulose fibres causing a grey/worn look, and by measuring the grey scale value a quantitative expression for the enzyme treatment is obtained. The results are represented in relation to the L-coordinate of a surface not treated with cellulase:
Composition: 1 2 3 4
Delta L 0.62 0.72 1.58 0.41
The relative standard deviation of the above figures is 6%.
Claims
1. Process for treatment of textiles by use of an enzyme, characterized by the fact that the enzyme is used in a rinse liquor for a rinsing step after washing.
2. Process according to Claim 1, characterized by the fact that the enzyme is used in the rinse liquor for the rinsing step after washing together with a fabric softening agent and/or an antistatic agent.
3. Process according to Claims 1 - 2, wherein the fabric softening agent and/or the antistatic agent is a cationic surfactant.
4. Process according to Claims 1 - 3, wherein the enzyme is used in the rinse liquor for a second or later rinsing step, preferably the last rinsing step.
5. Process according to Claims 1 - 4, wherein the enzyme is a lipase and/or a cellulase and/or a protease and/or an amylase.
6. Process according to Claims 1 - 5, wherein the enzyme is a lipase and/or a cellulase.
7. Process according to Claims 1 - 6, wherein the enzyme(s) is (are) of microbial origin.
8. Process according to Claims 2 - 7, characterized by the fact that the enzyme is cellulase, that the softening agent and the antistatic agent is a cationic surfactant, and that the rinse liquor additionally contains a non-ionic surfactant.
9. Process according to Claims 1 - 8, wherein the concentration of enzyme in the rinse liquor is in the range 0.001 to 5, preferably 0.01 to 2 mg pure enzyme protein per liter of rinse liquor.
10. Rinsing agent for use in the process according to Claims 1 - 9, 5 characterized by the fact that the rinsing agent is an enzyme preparation stabilized against microbial infection.
11. Rinsing agent according to Claim 10, characterized by the fact that it also comprises a fabric softening agent and/or an antistatic agent.
12. Rinsing agent according to Claim 11, characterized by the fact that 10 the rinsing agent is aqueous and that it exhibits an enzyme activity between 0.01 and 50 mg pure enzyme protein per liter of rinsing agent, preferably between 0.5 and 10 mg pure enzyme protein per liter of rinsing agent, and by a content of at least 1% (by weight) of fabric softening and/or antistatic agent (% as active materi¬ al).
15 13. Rinsing agent according to Claim 11 - 12, characterizd by the fact that the enzyme is cellulase, that the softening agent and the antistatic agent is a cationic surfactant, and that the rinsing agent additionally contains a non-ionic surfactant.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE91905036T DE69100751T2 (en) | 1990-03-01 | 1991-02-28 | METHOD FOR TREATING TEXTILES. |
AT91905036T ATE98296T1 (en) | 1990-03-01 | 1991-02-28 | METHOD OF TREATMENT OF TEXTILES. |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK530/90 | 1990-03-01 | ||
DK53090A DK53090D0 (en) | 1990-03-01 | 1990-03-01 | |
DK148990A DK148990D0 (en) | 1990-06-19 | 1990-06-19 | PROCEDURE FOR THE TREATMENT OF TEXTILE MATERIALS AND RULE FOR USE IN THE PROCEDURE |
DK1489/90 | 1990-06-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1991013136A1 true WO1991013136A1 (en) | 1991-09-05 |
Family
ID=26064162
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1991/000057 WO1991013136A1 (en) | 1990-03-01 | 1991-02-28 | Process for treatment of textiles and rinsing agent for use in the process |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0517762B1 (en) |
JP (1) | JP2878454B2 (en) |
AT (1) | ATE98296T1 (en) |
DE (1) | DE69100751T2 (en) |
DK (1) | DK0517762T3 (en) |
ES (1) | ES2047400T3 (en) |
WO (1) | WO1991013136A1 (en) |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0646165A4 (en) * | 1991-01-16 | 1993-09-23 | Procter & Gamble | Detergent compositions containing lipase and water-soluble quaternary ammonium compounds. |
WO1995005442A1 (en) * | 1993-08-12 | 1995-02-23 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
WO1995005443A1 (en) * | 1993-08-12 | 1995-02-23 | The Procter & Gamble Company | Fabric-conditioning compositions |
WO1995011292A1 (en) * | 1993-10-22 | 1995-04-27 | Unilever Plc | Fabric conditioner composition |
US5445747A (en) * | 1994-08-05 | 1995-08-29 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
WO1995029980A1 (en) * | 1994-04-29 | 1995-11-09 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
US5650322A (en) * | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
US5707951A (en) * | 1995-03-03 | 1998-01-13 | The Procter & Gamble Company | Laundry composition containing dye fixatives and cellulase |
US5770104A (en) * | 1990-10-05 | 1998-06-23 | Genencor International, Inc. | Detergent compositions containing substantially pure EG III cellulase |
US6162782A (en) * | 1990-10-05 | 2000-12-19 | Genencor International, Inc. | Detergent compositions containing cellulase compositions deficient in CBH I type components |
US6251144B1 (en) | 1992-06-12 | 2001-06-26 | Genencor International, Inc. | Enzymatic compositions and methods for producing stonewashed look on indigo-dyed denim fabric and garments |
US20110138541A1 (en) * | 2009-12-15 | 2011-06-16 | Whirlpool Corporation | Method for dispensing an enzyme in a laundry treating appliance |
EP2987848A1 (en) * | 2014-08-19 | 2016-02-24 | The Procter & Gamble Company | Method of laundering a fabric |
EP3299505B1 (en) | 2016-09-21 | 2021-01-27 | Bolton Manitoba SpA | Optimized method for washing in washing machines |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69534513T2 (en) | 1994-03-08 | 2006-07-27 | Novozymes A/S | NOVEL ALKALINE CELLULASES |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1368599A (en) * | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
EP0220016A2 (en) * | 1985-10-08 | 1987-04-29 | Novo Nordisk A/S | Clarification agent for coloured fabrics and method for treatment of fabrics |
EP0276547A1 (en) * | 1986-11-04 | 1988-08-03 | The University Of Reading | Treatment of wool materials |
-
1991
- 1991-02-28 ES ES91905036T patent/ES2047400T3/en not_active Expired - Lifetime
- 1991-02-28 WO PCT/DK1991/000057 patent/WO1991013136A1/en active IP Right Grant
- 1991-02-28 JP JP3504593A patent/JP2878454B2/en not_active Expired - Lifetime
- 1991-02-28 DE DE91905036T patent/DE69100751T2/en not_active Expired - Fee Related
- 1991-02-28 DK DK91905036.9T patent/DK0517762T3/en active
- 1991-02-28 AT AT91905036T patent/ATE98296T1/en active
- 1991-02-28 EP EP91905036A patent/EP0517762B1/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1368599A (en) * | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
EP0220016A2 (en) * | 1985-10-08 | 1987-04-29 | Novo Nordisk A/S | Clarification agent for coloured fabrics and method for treatment of fabrics |
EP0276547A1 (en) * | 1986-11-04 | 1988-08-03 | The University Of Reading | Treatment of wool materials |
Cited By (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6162782A (en) * | 1990-10-05 | 2000-12-19 | Genencor International, Inc. | Detergent compositions containing cellulase compositions deficient in CBH I type components |
US5770104A (en) * | 1990-10-05 | 1998-06-23 | Genencor International, Inc. | Detergent compositions containing substantially pure EG III cellulase |
US5650322A (en) * | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
EP0646165A4 (en) * | 1991-01-16 | 1993-09-23 | Procter & Gamble | Detergent compositions containing lipase and water-soluble quaternary ammonium compounds. |
EP0646165A1 (en) * | 1991-01-16 | 1995-04-05 | The Procter & Gamble Company | Detergent compositions containing lipase and water-soluble quaternary ammonium compounds |
US6251144B1 (en) | 1992-06-12 | 2001-06-26 | Genencor International, Inc. | Enzymatic compositions and methods for producing stonewashed look on indigo-dyed denim fabric and garments |
TR27769A (en) * | 1993-08-12 | 1995-08-04 | Procter & Gamble | Fabric conditioning compositions prepared in a narrow acidic ph range. |
US5599786A (en) * | 1993-08-12 | 1997-02-04 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
US5616553A (en) * | 1993-08-12 | 1997-04-01 | The Procter & Gamble Company | Fabric conditioning compositions |
WO1995005443A1 (en) * | 1993-08-12 | 1995-02-23 | The Procter & Gamble Company | Fabric-conditioning compositions |
WO1995005442A1 (en) * | 1993-08-12 | 1995-02-23 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
CN1046957C (en) * | 1993-08-12 | 1999-12-01 | 普罗格特-甘布尔公司 | Cellulase fabric-conditioning compositions |
WO1995011292A1 (en) * | 1993-10-22 | 1995-04-27 | Unilever Plc | Fabric conditioner composition |
WO1995029980A1 (en) * | 1994-04-29 | 1995-11-09 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
US5721205A (en) * | 1994-04-29 | 1998-02-24 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
US5445747A (en) * | 1994-08-05 | 1995-08-29 | The Procter & Gamble Company | Cellulase fabric-conditioning compositions |
US5707951A (en) * | 1995-03-03 | 1998-01-13 | The Procter & Gamble Company | Laundry composition containing dye fixatives and cellulase |
US20110138541A1 (en) * | 2009-12-15 | 2011-06-16 | Whirlpool Corporation | Method for dispensing an enzyme in a laundry treating appliance |
US8533881B2 (en) * | 2009-12-15 | 2013-09-17 | Whirpool Corporation | Method for dispensing an enzyme in a laundry treating appliance |
US10006160B2 (en) | 2009-12-15 | 2018-06-26 | Whirlpool Corporation | Method for dispensing an enzyme in a laundry treating appliance |
US10724168B2 (en) | 2009-12-15 | 2020-07-28 | Whirlpool Corporation | Method for dispensing an enzyme in a laundry treating appliance |
EP2987848A1 (en) * | 2014-08-19 | 2016-02-24 | The Procter & Gamble Company | Method of laundering a fabric |
WO2016028599A1 (en) * | 2014-08-19 | 2016-02-25 | The Procter & Gamble Company | Method of laundering a fabric |
EP3299505B1 (en) | 2016-09-21 | 2021-01-27 | Bolton Manitoba SpA | Optimized method for washing in washing machines |
Also Published As
Publication number | Publication date |
---|---|
ATE98296T1 (en) | 1993-12-15 |
JPH05504367A (en) | 1993-07-08 |
JP2878454B2 (en) | 1999-04-05 |
EP0517762A1 (en) | 1992-12-16 |
DE69100751D1 (en) | 1994-01-20 |
DE69100751T2 (en) | 1994-03-24 |
ES2047400T3 (en) | 1994-02-16 |
DK0517762T3 (en) | 1994-04-18 |
EP0517762B1 (en) | 1993-12-08 |
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