WO1992012992A3 - Basic structural immunogenic polypeptides having epitopes for hcv, antibodies, polynucleotide sequences, vaccines and methods - Google Patents

Basic structural immunogenic polypeptides having epitopes for hcv, antibodies, polynucleotide sequences, vaccines and methods Download PDF

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Publication number
WO1992012992A3
WO1992012992A3 PCT/US1992/000356 US9200356W WO9212992A3 WO 1992012992 A3 WO1992012992 A3 WO 1992012992A3 US 9200356 W US9200356 W US 9200356W WO 9212992 A3 WO9212992 A3 WO 9212992A3
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WO
WIPO (PCT)
Prior art keywords
novel
polypeptides
hcv
immunogenic
fgb2
Prior art date
Application number
PCT/US1992/000356
Other languages
French (fr)
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WO1992012992A2 (en
Inventor
Girish J Kotwal
Bahige M Baroudy
Original Assignee
Gamble James N Med Inst
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Publication date
Application filed by Gamble James N Med Inst filed Critical Gamble James N Med Inst
Publication of WO1992012992A2 publication Critical patent/WO1992012992A2/en
Publication of WO1992012992A3 publication Critical patent/WO1992012992A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1081—Togaviridae, e.g. flavivirus, rubella virus, hog cholera virus
    • C07K16/109—Hepatitis C virus; Hepatitis G virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Novel basic immunogenic polypeptides having epitopes for HCV are disclosed. The novel basic immunogenic polypeptides are truncated polypeptides derived from the structural region of a human HCV isolate. Two preferred novel basic immunogenic polypeptides are designated as FGB1 and FGB2. The FGB1 and FGB2 polypeptides are believed to be derived from near the N- and C-terminals, respectively, of a putative (C) protein of HCV. In an ELISA, eight serum samples reactive for anti-C100 HCV antibody (EIA, Ortho/Chiron), recombinant immunoblot assay I (RIBA I, Ortho/Chiron) and neutralization (Neut., Abbot Labs.) contained antibodies to the FGB1 and FGB2 polypeptides. In addition, the FGB1 and FGB2 polypeptides and RIBA I were non-reactive with twelve serum samples which were reactive with EIA. Still further, two serum samples from a patient clinically diagnosed as NANBH, which were non-reactive with EIA and RIBA I, were reactive with FGB1 and/or FGB2. Novel polynucleotide sequences encoding the basic immunogenic polypeptides are also disclosed. The present invention further includes the application of these new sequences, polypeptides, and antibodies raised against the novel polypeptides in HCV detection, such as immunoassays, diagnostics, PCR technology and gene therapy. Included within the invention also are novel immunogenic HCV polypeptides encoded within recombinant expression systems and clones, novel transformants, novel probes, novel methods for producing the immunogenic HCV polypeptides, novel anti-idiotype antibodies, novel antisense polynucleotides, and novel kits.
PCT/US1992/000356 1991-01-14 1992-01-14 Basic structural immunogenic polypeptides having epitopes for hcv, antibodies, polynucleotide sequences, vaccines and methods WO1992012992A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63980991A 1991-01-14 1991-01-14
US639,809 1991-01-14

Publications (2)

Publication Number Publication Date
WO1992012992A2 WO1992012992A2 (en) 1992-08-06
WO1992012992A3 true WO1992012992A3 (en) 1993-03-18

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PCT/US1992/000356 WO1992012992A2 (en) 1991-01-14 1992-01-14 Basic structural immunogenic polypeptides having epitopes for hcv, antibodies, polynucleotide sequences, vaccines and methods

Country Status (3)

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EP (1) EP0571554A1 (en)
AU (1) AU1459792A (en)
WO (1) WO1992012992A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9105871D0 (en) * 1991-03-20 1991-05-08 Inst Cientifico Tecnol Navarra Synthetic peptides
DE4209215A1 (en) 1991-07-04 1993-01-07 Boehringer Mannheim Gmbh HCV PEPTIDE ANTIGEN AND METHOD FOR DETERMINING HCV
WO1993007488A1 (en) * 1991-10-02 1993-04-15 Eiken Kagaku Kabushiki Kaisha Reagent and method for testing hepatitis c
DE4240980A1 (en) * 1992-08-07 1994-02-10 Boehringer Mannheim Gmbh HCV peptide antigens and method for the determination of HCV
JPH06311885A (en) * 1992-08-25 1994-11-08 Mitsubishi Kasei Corp Anti-sense compound complementary with gene of hepatitis c virus
US6174868B1 (en) * 1992-09-10 2001-01-16 Isis Pharmaceuticals, Inc. Compositions and methods for treatment of hepatitis C virus-associated diseases
ATE202383T1 (en) * 1992-09-10 2001-07-15 Isis Pharmaceuticals Inc COMPOSITIONS AND METHODS FOR THE TREATMENT OF HEPATITIS C VIRUS-ASSOCIATED DISEASES
JPH10503364A (en) * 1994-05-10 1998-03-31 ザ ジェネラル ホスピタル コーポレーション Antisense inhibition of hepatitis C virus
AU707811B2 (en) * 1995-05-05 1999-07-22 Australian Water Technologies Pty Ltd Method for the detection of viable Cryptosporidium parvum oocysts
AUPN283195A0 (en) * 1995-05-05 1995-06-01 Australian Water Technologies Pty Ltd Method for the detection of viable cryptosporidium parvum cells
IT1285158B1 (en) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio SOLUBLE POLYPEPTIDES WITH THE SERINO-PROTEASIS ACTIVITY OF NS3 OF THE HEPATITIS C VIRUS, AND PROCEDURE FOR THEIR PREPARATION AND
AU4497999A (en) * 1998-04-28 1999-11-16 Ingrid-Corina Bergter Radioimmunopharmaca for treating hepatitis c

Citations (9)

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WO1990014436A1 (en) * 1989-05-18 1990-11-29 Chiron Corporation Nanbv diagnostics: polynucleotides useful for screening for hepatitis c virus
EP0442394A2 (en) * 1990-02-16 1991-08-21 United Biomedical, Inc. Synthetic peptides specific for the detection of antibodies to HCV, diagnosis of HCV infection and prevention thereof as vaccines
EP0445423A2 (en) * 1989-12-22 1991-09-11 Abbott Laboratories Hepatitis C assay
EP0451891A2 (en) * 1990-03-30 1991-10-16 Akzo Nobel N.V. Peptides immunochemically reactive with antibodies directed against hepatitis Non-A, Non-B virus
EP0471356A1 (en) * 1990-08-14 1992-02-19 JAPAN as represented by Director General of Agency of NATIONAL INSTITUTE OF HEALTH Antigenic peptide for detecting anti-hepatitis C virus antibodies and use thereof
WO1992002642A1 (en) * 1990-08-10 1992-02-20 Chiron Corporation Nanbv diagnostics: polynucleotides useful for screening for hepatitis c virus
EP0479376A1 (en) * 1990-10-05 1992-04-08 Akzo Nobel N.V. Peptides immunochemically reactive with antibodies directed against hepatitis Non-A, Non-B virus
EP0485209A1 (en) * 1990-11-08 1992-05-13 Immuno Japan Inc. Non A, non B hepatitis virus related antigen, antibody detection systems, polynucleotides and polypeptides
EP0489968A1 (en) * 1990-12-14 1992-06-17 Innogenetics N.V. Synthetic antigens for the detection of antibodies to hepatitis C virus

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014436A1 (en) * 1989-05-18 1990-11-29 Chiron Corporation Nanbv diagnostics: polynucleotides useful for screening for hepatitis c virus
EP0445423A2 (en) * 1989-12-22 1991-09-11 Abbott Laboratories Hepatitis C assay
EP0442394A2 (en) * 1990-02-16 1991-08-21 United Biomedical, Inc. Synthetic peptides specific for the detection of antibodies to HCV, diagnosis of HCV infection and prevention thereof as vaccines
EP0451891A2 (en) * 1990-03-30 1991-10-16 Akzo Nobel N.V. Peptides immunochemically reactive with antibodies directed against hepatitis Non-A, Non-B virus
WO1992002642A1 (en) * 1990-08-10 1992-02-20 Chiron Corporation Nanbv diagnostics: polynucleotides useful for screening for hepatitis c virus
EP0471356A1 (en) * 1990-08-14 1992-02-19 JAPAN as represented by Director General of Agency of NATIONAL INSTITUTE OF HEALTH Antigenic peptide for detecting anti-hepatitis C virus antibodies and use thereof
EP0479376A1 (en) * 1990-10-05 1992-04-08 Akzo Nobel N.V. Peptides immunochemically reactive with antibodies directed against hepatitis Non-A, Non-B virus
EP0485209A1 (en) * 1990-11-08 1992-05-13 Immuno Japan Inc. Non A, non B hepatitis virus related antigen, antibody detection systems, polynucleotides and polypeptides
EP0489968A1 (en) * 1990-12-14 1992-06-17 Innogenetics N.V. Synthetic antigens for the detection of antibodies to hepatitis C virus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biochemical and Biophysical Research Communications, vol. 172, no. 2, 30 October 1990, Academic Press, Inc., K. MURAISO et al.: "A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus", pages 511-516, see the whole document *
Japan. J. Exp. Med., vol. 60, no. 3, June 1990, H. OKAMOTO et al.: "The 5'-terminal sequence of the hepatitis C virus genome", pages 167-177 *
Journal of General Virology, vol. 71, December 1990, (GB), K. TAKEUCHI et al.: "The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers", pages 3027-3033, see page 3032, column 2, last paragraph *
Proceedings of the 28th Symposium on Peptide Chemistry, Osaka, 25-27 October 1990, (ed. Y. SHIMONISHI), E. MUNEKATA et al.: "Epitope mapping of hepatitis C virus constituting protein", pages 211-214, see the whole document *

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Publication number Publication date
EP0571554A1 (en) 1993-12-01
AU1459792A (en) 1992-08-27
WO1992012992A2 (en) 1992-08-06

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