WO1992019249A1 - Compositions for the prevention and/or treatment of pathological processes - Google Patents
Compositions for the prevention and/or treatment of pathological processes Download PDFInfo
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- WO1992019249A1 WO1992019249A1 PCT/US1992/003626 US9203626W WO9219249A1 WO 1992019249 A1 WO1992019249 A1 WO 1992019249A1 US 9203626 W US9203626 W US 9203626W WO 9219249 A1 WO9219249 A1 WO 9219249A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7024—Esters of saccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/727—Heparin; Heparan
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
Definitions
- the present invention relates to pharmaceutical compositions for the prevention and/or treatment of pathological processes involving induction of Tumor Necrosis Factor alpha (TNF- ⁇ ) secretion, which comprise an effective low dosage of a low molecular weight heparin (LMWH) to be administered at intervals of between five to eight days.
- TNF- ⁇ Tumor Necrosis Factor alpha
- LMWH low molecular weight heparin
- the LMWHs to be used according to the invention have an average molecular weight of from about 3,000 to about 6,000 and inhibit TNF- ⁇ secretion by resting T cells and/or macrophages in vitro in response to activation by T cell-specific antigens, T cell mitogens, macrophage activators, disrupted extracellular matrix (dECM) , fibronectin, laminin or other ECM components.
- T cell-specific antigens T cell mitogens, macrophage activators, disrupted extracellular matrix (dECM) , fibronectin, laminin or other ECM components.
- dECM disrupted extracellular matrix
- TNF- ⁇ a cytokine produced by monocytes (macrophages) and T lymphocytes
- monocytes macrophages
- T lymphocytes T lymphocytes
- TNF- ⁇ The biologic effects of TNF- ⁇ depend on its concentration and site of production: at low concentrations, TNF- ⁇ may produce desirable homeostatic and defense functions, but at high concentrations, systemically or in certain tissues, TNF- ⁇ can synergize with other cytokines, notably interleukin-1 ( ⁇ L-1) to aggravate many inflammatory responses.
- cytokines notably interleukin-1 ( ⁇ L-1) to aggravate many inflammatory responses.
- TNF- ⁇ (together with IL-1) ; fever, slow-wave sleep, he odynamic shock, increased production of acute phase proteins, decreased production of albumin, activation of vascular endothelial cells, increased expression of major histocompatibility complex (MHC) molecules, decreased lipoprotein lipase, decreased cytochrome P450, decreased plasma zinc and iron, fibroblast proliferation, increased synovial cell collagenase, increased cyclo-oxygenase activity, activation of T cells and B cells, and induction of secretion of the cytokines, TNF- ⁇ itself, IL-1 and IL-6.
- MHC major histocompatibility complex
- TNF- ⁇ exerts its effects is not known in detail, but many of the effects are thought to be related to the ability of TNF- ⁇ to stimulate cells to produce prostaglandins and leukotrienes from arachidonic acid of the cell membrane.
- TNF- ⁇ As a result of its pleiotropic effects, has been implicated in a variety of pathologic states in many different organs of the body.
- TNF- ⁇ promotes hemorrhagic shock, down regulates endothelial cell thrombomodulin and enhances a procoagulant activity. It causes the adhesion of white blood cells and probably of platelets to the walls of blood vessels, and so, may promote processes leading to atherosclerosis, as well as to vasculitis.
- TNF- ⁇ activates blood cells and causes the adhesion of neutrophils, eosinophils, monocytes/ macrophages and T and B lymphocytes.
- TNF- ⁇ augments the chemotaxis of inflammatory cells and their penetration into tissues.
- TNF- ⁇ has a role in the tissue damage of autoimmune diseases, allergies and graft rejection.
- TNF- ⁇ has also been called cachectin because it modulates the metabolic activities of adipocytes and contributes to the wasting and cachexia accompanying cancer, chronic infections, chronic heart - _? -
- TNF- ⁇ may also have a role in anorexia nervosa by inhibiting appetite while enhancing wasting of fatty tissue.
- TNF- ⁇ has metabolic effects on skeletal and cardiac muscle. It has also marked effects on the liver: it depresses albumin and cytochrome P450 metabolism and increases production of fibrinogen, ⁇ l-acid glycoprotein and other acute phase proteins. It can also cause necrosis of the bowel. In the central nervous system, TNF- ⁇ crosses the blood-brain barrier and induces fever, increased sleep and anorexia. Increased TNF- ⁇ concentration is associated with multiple sclerosis. It further causes adrenal hemorrhage and affects production of steroid hormones, enhances collagenase and PGE-2 in the skin, and causes the breakdown of bone and cartilage by activating osteoclasts.
- TNF- ⁇ is involved in the pathogenesis of many undesirable inflammatory conditions in autoimmune diseases, graft rejection, vasculitis and atherosclerosis. It may have roles in heart failure, in the response to cancer and in anorexia nervosa. For these reasons, ways have been sought to regulate the secretion of TNF- ⁇ as a means to control a variety of diseases.
- the prime function of the immune system is to protect the individual against infection by foreign invaders such as microorganisms. It may, however, also attack the individual's own tissues leading to pathologic states known as autoimmune diseases.
- the aggressive reactions of an individual's immune system against tissues from other individuals are the reasons behind the unwanted rejections of transplanted organs. Hyper-reactivity of the system against foreign substances causes allergy giving symptoms like asthma, rhinitis and eczema.
- the cells mastering these reactions are the lymphocytes, primarily the activated T lymphocytes, and the pathologic inflammatory response they direct depends on their ability to traffic through blood vessel walls to and from their target tissue. Thus, reducing the ability of lymphocytes to adhere to and penetrate through the walls of blood vessels may prevent autoimmune attack, graft rejection and allergy. This would represent a new therapeutic principle likely to result in better efficacy and reduced adverse reactions compared to the therapies used today.
- Atherosclerosis and vasculitis are chronic and acute examples of pathological vessel inflammation.
- Atherosclerosis involves thickening and rigidity of the intima of the arteries leading to coronary diseases, myocardial infarction, cerebral infarction and peripheral vascular diseases, and represents a major cause of morbidity and mortality in the Western world.
- Pathologically, atherosclerosis develops slowly and chronically as a lesion caused by fatty and calcareous deposits. The proliferation of fibrous tissues leads ultimately to an acute condition producing sudden occlusion of the lumen of the blood vessel.
- TNF- ⁇ has been shown to facilitate and augment human immunodeficiency virus (HIV) replication in vitro (Matsuyama, T. et al., J. Virol. (1989) 63(6) :2504-2509; Michihiko, S. et al., Lancet (1989) 1(8648) :1206-1207) and to stimulate HIV-1 gene expression, thus, probably triggering the development of clinical AIDS in individuals latently infected with HIV-1 (Okamoto, T. et al., AIDS Res. Hum. Retroviruses (1989) 5X21:131-138).
- HIV human immunodeficiency virus
- Heparin is a glycosaminoglycan, a polyanionic ⁇ ulfated polysaccharide, which is used clinically to prevent blood clotting as an antithrombotic agent.
- models heparin has been shown to reduce the ability of autoimmune T lymphocytes to reach their target organ (Lider, 0. et al., Eur. J. Immunol. (1990) 2JD:493-499) .
- Heparin was also shown to suppress experimental autoimmune diseases in rats and to prolong the allograft survival in a model of skin transplantation in mice, when used in low doses (5 ⁇ g for mice and 20 ⁇ g for rats) injected once a day (Lider, O. et al., J. Clin. Invest. (1989) 82:752-756).
- the mechanisms behind the observed effects are thought to involve inhibition of release by T lymphocytes of enzyme(s) necessary for penetration of the vessel wall, primarily the enzyme heparanase that specifically attacks the glycosaminoglycan moiety of the sub-endothelial extracellular matrix (ECM) that lines blood vessels (Naparstek, Y. et al., Nature (1984) 310:241-243) .
- ECM sub-endothelial extracellular matrix
- Expression of the heparanase enzyme is associated with the ability of autoimmune T lymphocytes to penetrate blood vessel walls and to attack the brain in the model disease experimental autoimmune encephalomyelitis (EAE) .
- EP 0114589 (Folkman et al.) describes a composition for inhibition of angiogenesis in mammals in which the active agents consist essentially of (1) heparin or a heparin fragment which is a hexasaccharide or larger and (2) cortisone or hydrocortisone or the 11- ⁇ isomer of hydrocortisone. According to the disclosure, heparin by itself or cortisone by itself are ineffective; only the combination of both gives the desired effects.
- PCT Patent Application PCT/AU88/00017 published under No. WO88/05301 (Parish et al.) describes sulphated polysaccharides that block or inhibit endoglycosylase activity, such as heparanase activity, for use as antimetastatic and anti-inflammatory agents.
- Heparin and heparin derivatives such as periodate oxidized, reduced heparins, that had negligible anticoagulant activity, were shown to have antimetastatic and anti-inflammatory activity when used in dosages within, the range of 1.6-6.6 g per rat daily, administered by constant infusion (corresponding to 75-308 mg daily for an adult human patient) .
- the present invention is related to pharmaceutical compositions for the prevention and/or treatment of pathological processes involving the induction of TNF- ⁇ secretion comprising a pharmaceutically acceptable carrier and a low - -
- LMWH molecular weight heparin
- the LMWH of the pharmaceutical composition has an average molecular weight of from about 3,000 to about 6,000 and, furthermore, may be administered every fifth or seventh day.
- compositions to be administered at intervals of about 5-8 days for the prevention and/or treatment of pathological processes involving the induction of TNF- ⁇ secretion
- DTH delayed type hypersensitivity
- the applied antigen include, but are not limited to, tetanus, myelin basic protein, purified protein derivative, oxaz lone, and the like.
- compositions may be administered in any manner as dictated by the partici.lar application at hand including, but not limited to, enteral administration (including oral) or parenteral administration (including topical or inhalation with the aid of aerosols) .
- enteral administration including oral
- parenteral administration including topical or inhalation with the aid of aerosols
- the pharmaceutical compositions of the present invention are administered subcutaneously or intravenously.
- the present invention is useful, for example, in delaying or preventing allograft rejection and treating or preventing a variety of pathological processes such as those related to autoimmune diseases, allergy, inflammatory diseases (in particular, inflammatory bowel disease) , or acquired immunodeficiency syndrome (AIDS) .
- the present invention also finds utility in the treatment of diabetes type I, periodontal disease, uveitis, rheumatic diseases (in particular, rheumatoid arthritis) , atherosclerosis, vasculitis, or multiple sclerosis.
- compositions of the present invention contain low effective doses of the prescribed LMWH and typically contain a single low dose unit of less than 5 mg of LMWH, preferably from about 0.3 to about 3 mg, and most preferably contain a single low dose unit of from 1 to 1.5 mg.
- the present invention also contemplates broadly a method of using a low molecular weight heparin (LMWH) which is capable of inhibiting in vitro TNF- ⁇ secretion by resting T cells and/or macrophages in response to T cell-specific antigens, mitogens, macrophage activators, disrupted extracellular matrix (dECM) , la inin, fibronectin, or other ECM components for the preparation of a pharmaceutical composition to be administered at intervals of about 5-8 days for the prevention and/or treatment of pathological processes involving induction of TNF- ⁇ secretion which method comprises combining a low effective dose of the LMWH with a pharmaceutically acceptable carrier.
- a still further object of the present invention is to provide a method of treating a subject suffering from a pathological process involving induction of TNF- ⁇ secretion comprising administering to such subject a pharmaceutical composition, as described above, at intervals of about 5-8 days.
- LMWHs low molecular weight heparins
- DTH delayed type hypersensitivity
- AA adjuvant arthritis
- LMWH treatment also prolonged the survival of allogeneic heart transplants in rats and abrogated insulin dependent diabetes mellitus (IDDM) in NOD mice.
- treatment with LMWHs prevented the induction of TNF- ⁇ production by T cells and macroph"ges in response to the stimulus of damaged subendoti ..iial extracellular matrix (ECM) .
- ECM extracellular matrix
- TNF- ⁇ at the site of vascular injury has probably a role in the process of atherosclerosis
- inhibition of TNF- ⁇ at the site of damaged subendothelial ECM will ameliorate the pathogenic process of atherosclerosis.
- a most surprising aspect of treatment with LMWHs is that such treatment is most e fective when administered at low doses at weekly intervals. High doses of LMWHs or doses of LMWHs given daily are not effective in inhibiting TNF- ⁇ secretion or immune reactions.
- LMWHs administered at subanticoagulant doses at several day intervals are effective in the prevention and/or treatment of pathological processes involving induction of TNF- ⁇ .
- the LMWHs to be used according to the invention are derived from LMWHs with an average molecular weight of 3000-6000, such as, for example the LMWHs disclosed in European Patent EP 0014184.
- Some LMWHs are commercially available under different trade names, e.g., Fragmin®, Fraxiparin®, Fraxiparine®, Lovenox®/Clexane®.
- LMWHs can be produced in several different ways: enrichment by fractionation by ethanol and/or molecular sieving, e.g., gel filtration or membrane filtration of the LMWH present in standard heparin and controlled chemical (by nitrous acid, jS-elimination or periodate oxidation) or enzymatic (by heparinases) depolymerization.
- the conditions for depolymerization can be carefully controlled to yield products of desired molecular weights. Nitrous acid depolymerization is commonly used.
- depolymerization of the benzylic ester of heparin by 3-elimination which yields the same type of fragments as enzymatic depolymerization using heparinases.
- LMWH with low anticoagulant activity and retaining basic chemical structure can be prepared by depolymerization using periodate oxidation or by removing the antithrombin-binding fraction of LMWH, prepared by other methods, using immobilized antithrombin for adsorption.
- Fragmin® is a low molecular weight heparin with average molecular weight within the range of
- Fraxiparin®, and Fraxiparine® are LMWHs with average molecular weight of approximately 4500 dalton, produced by fractionation or controlled nitrous acid depolymerization, respectively, of calcium heparin from porcine intestinal mucosa. It is manufactured by Sanofi (Choay Laboratories) for use as an antithrombo- tic agent in single doses comprising ca. 36 mg, corresponding to 3075 IU/0.3 ml of water.
- Lovenox® (Enoxaparin/e) , a LMWH fragment produced by depolymerization of sodium heparin from porcine intestinal mucosa, using (3-elimination, is manufactured by Pharmuka SF, France and distributed by Rhone-Poulenc under the names Clexane® and Lovenox® for use as antithrombotic agent in single dose syringes comprising 20 mg/0.2 ml and 40 mg/0.4 ml of water.
- the novel properties of LMWHs described are a common feature of all, LMWHs regardless of the manufacturing process, the structural differences (created by depolymerization or those dependent on variation in the heparin used as raw material) or the anticoagulant activity, provided the LMWH employed is capable of inhibiting TNF- ⁇ secretion in vitro by resting T cells and/or macrophages in response to activation by contact with specific antigens, mitogens, disrupted ECM or its protein components, such as fibronectin or laminin.
- Another test useful to identify the LMWHs that are effective for the purpose of the present invention is the inhibition of experimental delayed type hypersensitivity (DTH) skin reactions, a T lymphocyte dependent reaction, to a variety of antigens (for example, tetanus antigen, myelin basic protein (MBP) , purified protein derivative (PPD) , and oxazolone) .
- DTH delayed type hypersensitivity
- MBP myelin basic protein
- PPD purified protein derivative
- oxazolone oxazolone
- the LMWHs effective according to the invention are incorporated into pharmaceutical compositions, for example, as water solutions, possibly comprising sodium chloride, stabilizers and other suitable non-active ingredients.
- pharmaceutical compositions for example, as water solutions, possibly comprising sodium chloride, stabilizers and other suitable non-active ingredients.
- the preferable way of administration is by injection, subcutaneous or intravenous, but any other suitable mode of administration is encompassed by the invention.
- the LMWH is to be administered at intervals of five to eight days, preferably once a week.
- the disorders that can be prevented or treated according to the invention are all disorders linked in pathological processes involving induction of TNF- ⁇ secretion, including atherosclerosis and vasculitis and pathological processes related thereto; autoimmune diseases, e.g., rheumatoid arthritis, diabetes type I (IDDM) ; allergy; graft rejection; acute and chronic inflammatory diseases, e.g. uveitis, bowel inflammation; anorexia nervosa; hemorrhagic shock caused by septicemia, and opportunistic infections in AIDS - immunocompromised individuals.
- the LMWHs will suppress replication of HIV and/or enhance resistance against the opportunistic infections, thereby preventing the development of AIDS-related complex (ARC) .
- ARC AIDS-related complex
- LMWHs can have a narrow dose range of effectiveness, and that different preparations of
- LMWHs can show different optimal biological effects. Therefore, it was necessary to establish a Unit Dose for LMWH preparation to be used according to the invention, said unit dose being based on a bioassay of inhibition of TNF- ⁇ secretion by mouse spleen cells from mice treated with LMWH or by inhibition of DTH reactivity by t_,_ LMWH. It was found that the ability of a particular dose of LMWH to inhibit TNF- ⁇ secretion is positively correlated with its ability to inhibit the delayed type hypersensitivity (DTH) reaction. This, cell-mediated inflammatory reaction in vivo is important because it is an expression of the processes involved in autoimmune diseases, graft rejection, some types of blood vessel inflammation and allergy.
- DTH delayed type hypersensitivity
- the lowest dose of LMWH per kg causing inhibition of TNF- ⁇ , production or inhibition of DTH reactivity by at least 50% is considered to constitute 12 mouse inhibitory units per kg (12 u/kg) . Because r " the differences in surface area and metabolism L.tween mice and humans, humans should be treated with a lower dose of LMWH, and 12 u/kg in mouse is established to correspond to 1 u/kg in humans. For example, the dose of Fragmin® batch 38609 effective in inhibiting both TNF- ⁇ secretion and DTH reactivity is 5 ⁇ g per mouse administered weekly.
- the dose of Fragmin® 38609 equivalent to 12 u/kg is 200 ⁇ g/kg of mice.
- a human weighing about 70 kg would then be treated by a dose of about 1.2 mg given in a single dose subcutaneously once every 7 days. Since individual humans vary biologically, the optimal dose may be different from about 1.2 mg and will lie below 5 mg, particularly within the range of 0.3 to 3 mg.
- the dose of LMWH that should be effective in rats can be derived from the fact that the dose of LMWH per kg of rats is one-half the dose per kg of mice, i.e. 6 u/kg. For example, if 12 u of Fragmin® batch 38609 is 200 ⁇ g/kg, then the 6 u dose suitable for rats should be 100 ⁇ g/kg or 20 ⁇ g per 200 g rat, administered once a week.
- TNF- ⁇ bioassay is based on the cytotoxic effect of
- HeLa cells by TNF- ⁇ present in the supernatants of the cells is measured, the concentration of TNF- ⁇ in said supernatants being determined by comparison to titration curves of TNF- ⁇ exogenously added.
- Cell viability is determined by incubation with neutral red for two hours, washing away excess dye, extracting the neut._al red that was taken up by the cells with Sorenson's citrate buffer-ethanol mixture, and quantitating it colorimetrically at 570 nm with a Microelisa Autoreader.
- mice from mice treated with LMWH are obtained as follows: female mice of the BALB/c strain (25 grams, 2 months old) , at least 5 mice per group, are injected subcutaneously with various doses of LMWH, usually in the range of 0.5 to 20 ⁇ g per mouse. Five days later the mice are killed by cervical dislocation, the spleens are removed and suspensions of spleen cells, depleted of red blood cells, are assayed for the production of TNF- ⁇ in response to induction by dECM, Concanavalin A (Con A) or lipopolysaccharide (LPS) .
- dECM Concanavalin A
- LPS lipopolysaccharide
- mice are sensitized on the shaved abdominal skin with 100 ⁇ l of 3% oxazolone (OX) in acetone/olive oil (4:1, v:v) .
- OX oxazolone
- acetone/olive oil 4:1, v:v
- DTH sensitivity is elicited 5 days later as follows: mice are challenged with 20 ⁇ l of 0.5% OX (10 ⁇ l to each side of the ear) in acetone/olive oil. A constant area of the ear is measured immediately before challenge and 24 and 48 h later with a Mitutoyo engineer's micrometer. The individual measuring ear swelling is blinded to the identity of the groups of mice. The increment ( ⁇ ) of ear swelling is expressed as the mean in units of 10 "2 mm or 10 "4 inch ( ⁇ SE) depending on the micrometer that is ..ed. Percent inhibition is calculated as follows:
- mice are treated with LMWH as in Example 1, injected the day before primary sensitization to OX. On the fifth day after sensitization to OX, the mice are challenged to induce a DTH reaction, as described above.
- the positive control is the DTH reaction elicited in immunized mice in the absence of treatment with LMWH.
- the negative control is the background swelling produced by the antigen in immunized mice.
- Example 3 Induction of TNF- ⁇ Secretion by T Cells and Macrophages
- fibronectin (FN) or laminin (LN) Sigma were added to flat bottom 96-well plates (Costar) at a concentration of 1 ⁇ g/50 ⁇ l PBS per well and removed after 16 h. Remaining binding sites were blocked with BSA/PBS (10 5 mg/ml) which was added to the wells for 2 h and washed out.
- ECM-coated wells were prepared as follows: bovine corneal endothelial cells were cultured in flat bottom 96-well plates.
- dECM Disrupted ECM
- MBP 100 ⁇ g/ml
- Con A 2.5 ⁇ g/ l
- LPS 1 ⁇ g/ml
- the plates were incubated at 37 °C in a humidified incubator for 3 h. Subsequently, the contents of the wells (4 wells per experimental group) were collected, centrifuged, and the media were assayed for TNF- ⁇ secretion as in Example 1: supernatants of cultured macrophages and lymphocytes were added to cultures of HeLa cells, which are sensitive to killing by TNF- ⁇ , and death of these cells in the presence of the test media was calibrated in comparison to titration curves of exogenous added TNF- ⁇ . Cell death is examined by the release of neutral red dye from the preincubated HeLa cells. The results shown here represent data obtained from a total of six experiments that produced essentially similar results.
- Table 1 shows that T cells and macrophages cultured together can be induced to secrete TNF- ⁇ by contact with specific antigen MBP (group 4) , the mitogen Con A (group 6) or LPS (group 8) .
- TNF- ⁇ was also induced to be secreted by disrupted extracellular matrix (dECM; group 10) or by the ECM components fibronectin (FN; group 12) or laminin (LN group 14) .
- dECM disrupted extracellular matrix
- FN fibronectin
- LN group 14 laminin
- TNF- ⁇ secretion by T cells and macrophages is induced by specific antigen MBP, Con A, LPS or dECM components.
- TNF- ⁇ (yes) or without TNF- ⁇
- T cell and accessory cell cultures were prepared as described in Example 3. LMWH was added to the wells at the beginning of the cell culture. The levels of TNF- ⁇ were examined after 3 h of incubation.
- Table 2 shows that the presence of LMWH (Fragmin® batch 38609) in vitro inhibited the induction of TNF- ⁇ secretion induced by specific antigen (MBP; group 4) , mitogens (Con A and LPS; groups 6 and 8) , dECM or ECM components (groups 10, 12 and 14) . Since TNF- ⁇ secretion induced by dECM is likely to be involved in atherosclerosis, inhibition of TNF- ⁇ by LMWH will be beneficial in atherosclerosis.
- Example 5 To examine the effect of LMWH administered to mice in vivo on the secretion of TNF- ⁇ by spleen cells in vitro, the following experiment was conducted. BALB/c mice, 5 per group, were treated with various doses of LMWH (Fragmin® batch 38609) diluted in saline, injected subcutaneously. After one week, the animals were killed and their spleen cells, devoid of red blood cells, were examined for their ability to secrete TNF- ⁇ in response to control wells without dECM (A) or to wells coated with dECM (B) . Measuring the levels of TNF- ⁇ secretion was done as described in Example 1.
- LMWH Framin® batch 38609
- Table 3 shows the results which indicate that an injection of 5 ⁇ g of LMWH given once, 7 days earlier, inhibited TNF- ⁇ secretion induced by dECM. Higher or lower doses of LMWH were less effective. Thus, an optimal dose of LMWH administered in vivo a week earlier was effective. Table 3. Ex vivo inhibition of T cell mediated TNF- ⁇
- Table 4 shows that a 5 ⁇ g dose in vivo of the LMWH Fragmin® batch 38609 was also effective in inhibiting TNF- ⁇ secretion induced by LPS.
- BALB/c (4 mice per experimental group) mice were treated with the indicated amounts of LMWH diluted in saline and injected subcutaneously. After one week, the mice were injected intraperitoneally with 10 mg LPS, killed 4 hours later and their spleen cells, devoid of red blood cells, were subsequently cultured in dECM coated wells for 3 hours in a humidified incubator. The levels of TNF- ⁇ secreted in response to the dECM was measured in the supernatants of the cultures. The results are given in Table 4.
- mice with LMWH inhibits LPS mediated secretion of TNF- ⁇ by macrophages.
- mice were treated with the indicated LMWH administered subcutaneously in different concentrations. After one week, some of the mice were killed and the induction of TNF- ⁇ secretion in response to Con A activation in vitro was measured. The remaining mice were examined for their ability to respond to the antigen oxazolone. The results are expressed in Table 5 as percent inhibition compared to the responses of the LMWH untreated mice.
- factor X assay have different optimal doses for inhibition of TNF- ⁇ secretion.
- preparations of LMWH such as Clexane® batch 4096, which have no inhibitory effect on TNF- ⁇ , secretion at any of the doses tried. Therefore, it may be concluded that the antithrombotic effect of a LMWH preparation is not related to the potential of the LMWH preparation for inhibition of TNF- ⁇ secretion.
- the two different bioassays are mediated by different factors present in the preparations.
- Adjuvant arthritis is an experimental disease inducible in some strains of rats by immunizing them to antigens of Mycobacterium tuberculosis (Pearson, C . , Proc. Soc. Exp. Biol.
- rats immunized to induce AA were injected subcutaneously weekly, starting on the 5th day after injection with 0.5 ⁇ g (o) , 1 ⁇ g ( ⁇ ) , 2 ⁇ g (•), 10 ⁇ g (0) , 15 ⁇ g ( ⁇ ) 20 ⁇ g (1); 30 ⁇ g (A) , 40 ⁇ g (x) and PBS control (D) .
- the dose was maximally effective in inhibiting arthritis.
- Fig. 2 The effect of the 20 ⁇ g dose of Fragmin® on the course of AA is shown in Fig. 2: PBS control (D) ; single treatment on 5th day (A) ; daily (•) ; every 5th day (o) ; weekly (1) . It is shown that Fragmin administration both at 5 day intervals and at 7 day intervals inhibits arthritis.
- Fig. 3 shows the effect of weekly administration of Fragmin® (batch 38609) as compared to standard heparin on AA.
- Lewis rats were immunized to induce AA. Beginning on day 5, the rats were inoculated subcutaneously at weekly intervals with a 20 ⁇ g dose of Fragmin® (•) , heparin (o) or phosphate buffered saline (PBS) control (D) .
- the results show a dramatic difference in potency between Fragmin® and heparin: Fragmin® completely inhibited arthritis, while heparin had no inhibitory effect.
- FIG. 5 shows the results of the injection of a 20 ⁇ g dose of Fraxiparin® (daily ( ⁇ ) ; weekly (!) ; Fraxiparine® (daily ( ⁇ ) ; weekly (A)), Lovenox®/Clexane® (daily (•) ; weekly (o)) , and PBS control (x) . All the three LMWHs of different types and sources showed a marked inhibition of arthritis, when administered weekly, but not daily.
- Wistar rats were subjected to allogeneic BN heart transplant (Ono, K. and Linsay, E.S., J. Thorac. Cardiovasc. Sur . (1969) 4J5 . :225-229) . From the day before transplantation, the rats were injected subcutaneously at 7 day intervals with 20 ⁇ g of
- IDDM insulin dependent diabetes mellitus
- a group of 10 female NOD mice was treated with weekly subcutaneous injections of 5 ⁇ g per mouse of Fragmin® (batch 38609) , the dose determined to represent 12 mouse units per kg.
- a group of 10 control mice were treated with injections of saline. At the age of 5 months all the mice were bled to determine the development of IDDM using a standard procedure (Elias, D. et al., Proc. Natl. Acad. Sci. U.S.A. (1990) 82:1576-1580).
- Fig. 7 shows that the control mice ("none") had abnormal blood glucose (400 mg/dl) .
- the mice treated with LMWH had a normal blood glucose (100 mg/dl) .
- treatment with LMWH can indeed cure the IDDM process.
- Example 10 LMWH Treatment of Human DTH Figure 8 shows an experiment in which a 40 year old male volunteer weighing 85 kg was tested for DTH reactivity to tetanus antigen (Merieux skin test applicator) . About 18 mm of induration was measured at 24 and 48 hours. The volunteer was then treated with a subcutaneous injection of Fragmin® (batch 38609) 3 mg. Five days later the volunteer was again tested for his DTH response to tetanus and the induration was inhibited to about 5 mm. The volunteer was tested again for DTH 3 weeks later ("Recovery") and the test showed positive reactivity (23 mm of induration at 24 and 48 hours) .
- Fragmin® Fragmin®
- LMWH LMWH at a dose of less than 5 mg can inhibit DTH in humans at treatment intervals of 5 and 7 days.
Abstract
Description
Claims
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
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DE69232615T DE69232615T2 (en) | 1991-05-02 | 1992-05-01 | COMPOSITIONS FOR PREVENTING AND / OR TREATING PATHOLOGICAL PROCESSES |
AT92911373T ATE217796T1 (en) | 1991-05-02 | 1992-05-01 | COMPOSITIONS FOR PREVENTING AND/OR TREATING PATHOLOGICAL PROCESSES |
JP4511483A JPH06507635A (en) | 1991-05-02 | 1992-05-01 | Compositions for the prevention and/or treatment of pathological processes |
KR1019930703303A KR100235782B1 (en) | 1991-05-02 | 1992-05-01 | Compositions for the prevention and/or treatment of pathological processes |
CS932325A CZ232593A3 (en) | 1991-05-02 | 1992-05-01 | Pharmaceutical preparation for preventing and/or therapy of pathological states |
SK1201-93A SK120193A3 (en) | 1991-05-02 | 1992-05-01 | Pharmaceutical composition for the prevention and/or treatment of pathological processes |
AU19131/92A AU668865B2 (en) | 1991-05-02 | 1992-05-01 | Compositions for the prevention and/or treatment of pathological processes |
EP92911373A EP0583360B1 (en) | 1991-05-02 | 1992-05-01 | Compositions for the prevention and/or treatment of pathological processes |
BR9205961A BR9205961A (en) | 1991-05-02 | 1992-05-01 | Pharmaceutical composition for the prevention and / or treatment of pathological processes, process of using a low molecular weight heparin for the preparation of a pharmaceutical composition and process of treating a person suffering from a pathological process |
NO933942A NO933942L (en) | 1991-05-02 | 1993-11-01 | Compositions for the prevention and / or treatment of pathological processes |
FI934849A FI934849A (en) | 1991-05-02 | 1993-11-02 | SAMMANSAETTNINGAR FOER FOERHINDRANDE OCH / ELLER BEHANDLANDE AV PATOLOGISKAPROCESSER |
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IL98028 | 1991-05-02 | ||
IL9802891A IL98028A (en) | 1991-05-02 | 1991-05-02 | Pharmaceutical compositions comprising a low molecular weight heparin (LMWH) |
IL98298 | 1991-05-28 | ||
IL98298A IL98298A0 (en) | 1991-05-28 | 1991-05-28 | Pharmaceutical compositions comprising a heparin derivative |
Publications (1)
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WO1992019249A1 true WO1992019249A1 (en) | 1992-11-12 |
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PCT/US1992/003626 WO1992019249A1 (en) | 1991-05-02 | 1992-05-01 | Compositions for the prevention and/or treatment of pathological processes |
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US (3) | US5474987A (en) |
EP (1) | EP0583360B1 (en) |
JP (1) | JPH06507635A (en) |
KR (1) | KR100235782B1 (en) |
AT (1) | ATE217796T1 (en) |
AU (1) | AU668865B2 (en) |
BR (1) | BR9205961A (en) |
CA (1) | CA2102207A1 (en) |
CZ (1) | CZ232593A3 (en) |
DE (1) | DE69232615T2 (en) |
FI (1) | FI934849A (en) |
HU (1) | HUT67136A (en) |
NO (1) | NO933942L (en) |
SK (1) | SK120193A3 (en) |
WO (1) | WO1992019249A1 (en) |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4281108A (en) * | 1980-01-28 | 1981-07-28 | Hepar Industries, Inc. | Process for obtaining low molecular weight heparins endowed with elevated pharmacological properties, and product so obtained |
US4788307A (en) * | 1986-04-30 | 1988-11-29 | Choay S.A. | Oligosaccharidic fractions devoid or practically devoid of antithrombotic activity |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL61201A (en) * | 1979-10-05 | 1984-09-30 | Choay Sa | Oligosaccharides having no more than 8 saccharide moieties,their obtention from heparin and pharmaceutical compositions containing them |
CA1226816A (en) * | 1982-12-20 | 1987-09-15 | Moses J. Folkman | Inhibition of angiogenesis |
US4916219A (en) * | 1985-03-28 | 1990-04-10 | University Of Iowa Research Foundation | Oligosaccharide heparin fragments as inhibitors of complement cascade |
IT1214609B (en) * | 1985-05-17 | 1990-01-18 | Opocrin Spa | HEXOSAMINOGLICANS DEPOLYMERIZED SULPHATES FOR ANTI-THROMBOTIC, FIBRINOLITHIC, ANTI-INFLAMMATORY ACTIVITIES, THEIR PREPARATION PROCEDURE AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM. |
US4889808A (en) * | 1985-10-01 | 1989-12-26 | American Home Products | Method of enchancing t-PA and SCU-PA production |
EP0240098A3 (en) * | 1986-04-04 | 1989-05-10 | Kabushiki Kaisha Ueno Seiyaku Oyo Kenkyujo | Oligo and polysaccharides for the treatment of diseases caused by retroviruses |
DE3787996T2 (en) * | 1986-05-16 | 1994-03-03 | Italfarmaco Spa | Heparin free from E.D.T.A., fractions and fragments of heparin, processes for their preparation and pharmaceutical compositions containing them. |
WO1988000017A1 (en) * | 1986-06-27 | 1988-01-14 | Boon Corporation Pty. Ltd. | Mat |
IL85145A (en) * | 1987-01-23 | 1994-08-26 | Univ Australian | Anti-metastatic pharmaceutical or veterinary compositions containing modified heparin having reduced anticoagulant activity |
FR2614026B1 (en) * | 1987-04-16 | 1992-04-17 | Sanofi Sa | LOW MOLECULAR WEIGHT HEPARINS WITH REGULAR STRUCTURE, THEIR PREPARATION AND THEIR BIOLOGICAL APPLICATIONS |
SE8702254D0 (en) * | 1987-05-29 | 1987-05-29 | Kabivitrum Ab | NOVEL HEPARIN DERIVATIVES |
AU4426289A (en) * | 1988-10-05 | 1990-05-01 | Nicola Diferrante | Methods for interfering with hiv multiplication and composition for the treatment of aids |
IL88554A0 (en) * | 1988-12-01 | 1989-07-31 | Hadassah Med Org | Compositions containing a compound binding to a heparin receptor |
PT93847A (en) * | 1989-04-24 | 1990-11-20 | Harvard College | PROCESS FOR THE PREPARATION OF OLIGOSACARIDES OF LOW MOLECULAR WEIGHT DERIVED FROM HEPARIN OR DESPOLYMENED HEPARAN SULFATE AND PHARMACEUTICAL COMPOSITIONS THAT CONTAIN THEM |
AT500533B8 (en) * | 2000-04-13 | 2007-02-15 | Man Nutzfahrzeuge Ag | GAS OR TROLLEY BUS |
-
1992
- 1992-05-01 SK SK1201-93A patent/SK120193A3/en unknown
- 1992-05-01 CZ CS932325A patent/CZ232593A3/en unknown
- 1992-05-01 BR BR9205961A patent/BR9205961A/en not_active Application Discontinuation
- 1992-05-01 AU AU19131/92A patent/AU668865B2/en not_active Ceased
- 1992-05-01 CA CA002102207A patent/CA2102207A1/en not_active Abandoned
- 1992-05-01 DE DE69232615T patent/DE69232615T2/en not_active Expired - Lifetime
- 1992-05-01 HU HU9303110A patent/HUT67136A/en unknown
- 1992-05-01 WO PCT/US1992/003626 patent/WO1992019249A1/en active IP Right Grant
- 1992-05-01 EP EP92911373A patent/EP0583360B1/en not_active Expired - Lifetime
- 1992-05-01 JP JP4511483A patent/JPH06507635A/en active Pending
- 1992-05-01 KR KR1019930703303A patent/KR100235782B1/en not_active IP Right Cessation
- 1992-05-01 AT AT92911373T patent/ATE217796T1/en not_active IP Right Cessation
-
1993
- 1993-11-01 NO NO933942A patent/NO933942L/en unknown
- 1993-11-02 FI FI934849A patent/FI934849A/en not_active Application Discontinuation
-
1995
- 1995-02-03 US US08/384,203 patent/US5474987A/en not_active Expired - Lifetime
- 1995-06-01 US US08/457,655 patent/US5686431A/en not_active Expired - Lifetime
-
1997
- 1997-11-07 US US08/966,315 patent/US5908837A/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4281108A (en) * | 1980-01-28 | 1981-07-28 | Hepar Industries, Inc. | Process for obtaining low molecular weight heparins endowed with elevated pharmacological properties, and product so obtained |
US4788307A (en) * | 1986-04-30 | 1988-11-29 | Choay S.A. | Oligosaccharidic fractions devoid or practically devoid of antithrombotic activity |
Non-Patent Citations (6)
Title |
---|
American Journal of Pathology, Vol. 82, No. 1, issued January 1976, R.W. COLMAN et al., "Hyperacute Renal Allograft Rejection in the Primate", pages 25-42, see entire document. * |
Annals of the New York Academy of Sciences, Vol. 463, (Colloq. Biol. Sci. 2nd), issued 1986, I.D. GOLDBERG et al., "The in vivo Effects of Heparin Fractions on the Development of Lung Metastasis in Murine Fibrosarcoma", pages 289-291, see entire document. * |
Cellular Immunology, Vol. 82, issued 1983, M.S. Sy et al., "Inhibition of Delayed-Type Hypersensitivity by Heparin Depleted of Anticoagulant Activity", pages 23-32, 24-29. * |
Physiology, Chemistry and Physics, Vol. 10, issued 1978, I. KIRICUTA et al., "Prophylaxis of Metastases Formation by Prolonged Non-specific Immunostimulation Associated with Heparin Therapy", pages 247-253, see entire document. * |
The Journal of Biological Chemistry, Vol. 263, No. 26, issued 15 September 1988, R.J. LINHARDT et al., "Homogeneous, Structurally Defined Heparin-Oligosaccharides with Low Anticoagulant Activity Inhibit the Generation of the Amplification Pathway C3 Convertase in Vitro", pages 13090-13096, see entire document. * |
Thrombosis Research, Vol. 42, issued 1986, J. WESTWICK et al., "Comparison of the Effects of Low Molecular Weight Heparin and Unfractioned Heparin on Activation of Human Platelets in vitro", pages 435-447, see entire document. * |
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US6417173B1 (en) | 1991-05-31 | 2002-07-09 | Gliatech, Inc. | Methods and compositions based on inhibition of cell invasion and fibrosis by anionic polymers |
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WO1994018988A2 (en) * | 1993-02-22 | 1994-09-01 | Von Arnim Ulrich Christoph | Use of heparins for the treatment of inflammatory or immunological diseases |
WO1994018988A3 (en) * | 1993-02-22 | 1994-11-10 | Von Arnim Ulrich Christoph | Use of heparins for the treatment of inflammatory or immunological diseases |
US6103705A (en) * | 1996-11-27 | 2000-08-15 | Aventis Pharmaceuticals Products Inc. | Pharmaceutical composition comprising a compound having anti-Xa activity and a platelet aggregation antagonist compound |
US7390491B2 (en) | 1997-07-14 | 2008-06-24 | Takashi Muramatsu | Agents comprising midkine or an inhibitor thereof as active ingredient |
EP1003479A1 (en) * | 1997-08-04 | 2000-05-31 | Baker Norton Pharmaceuticals, Inc. | Method and compositions for treating late phase allergic reactions and inflammatory diseases |
EP1003479A4 (en) * | 1997-08-04 | 2001-01-17 | Baker Norton Pharma | Method and compositions for treating late phase allergic reactions and inflammatory diseases |
WO2000040225A3 (en) * | 1998-12-30 | 2000-09-21 | Applied Research Systems | Inhibition of tnf activity |
US6608044B1 (en) | 1998-12-30 | 2003-08-19 | Applied Research Systems Ars Holding N.V. | Inhibition of TNF activity |
WO2000040225A2 (en) * | 1998-12-30 | 2000-07-13 | Applied Research Systems Ars Holding N.V. | Inhibition of tnf activity |
WO2002047696A1 (en) * | 2000-12-16 | 2002-06-20 | Aventis Pharma Deutschland Gmbh | Use of low molecular heparin for treating osteoarthritis |
WO2009141821A1 (en) * | 2008-05-20 | 2009-11-26 | Crystal Clear Partnership | Separation of polysaccharides by charge density gradient |
Also Published As
Publication number | Publication date |
---|---|
NO933942D0 (en) | 1993-11-01 |
BR9205961A (en) | 1994-07-26 |
FI934849A (en) | 1993-12-31 |
HU9303110D0 (en) | 1994-01-28 |
US5686431A (en) | 1997-11-11 |
US5474987A (en) | 1995-12-12 |
ATE217796T1 (en) | 2002-06-15 |
HUT67136A (en) | 1995-02-28 |
KR100235782B1 (en) | 1999-12-15 |
EP0583360A1 (en) | 1994-02-23 |
JPH06507635A (en) | 1994-09-01 |
AU668865B2 (en) | 1996-05-23 |
EP0583360A4 (en) | 1994-04-06 |
SK120193A3 (en) | 1994-07-06 |
DE69232615T2 (en) | 2003-01-30 |
CA2102207A1 (en) | 1992-11-03 |
DE69232615D1 (en) | 2002-06-27 |
NO933942L (en) | 1993-12-14 |
CZ232593A3 (en) | 1994-07-13 |
EP0583360B1 (en) | 2002-05-22 |
AU1913192A (en) | 1992-12-21 |
US5908837A (en) | 1999-06-01 |
FI934849A0 (en) | 1993-11-02 |
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