WO1992021688A1 - New perfluoroalkylated amphiphilic phosphorus compounds: preparation and biomedical applications - Google Patents
New perfluoroalkylated amphiphilic phosphorus compounds: preparation and biomedical applications Download PDFInfo
- Publication number
- WO1992021688A1 WO1992021688A1 PCT/EP1992/001329 EP9201329W WO9221688A1 WO 1992021688 A1 WO1992021688 A1 WO 1992021688A1 EP 9201329 W EP9201329 W EP 9201329W WO 9221688 A1 WO9221688 A1 WO 9221688A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- phosphate
- octyl
- glucosyl
- ethyl
- hexyl
- Prior art date
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- 238000002360 preparation method Methods 0.000 title abstract description 33
- 150000003018 phosphorus compounds Chemical class 0.000 title abstract description 3
- 150000001875 compounds Chemical class 0.000 claims abstract description 116
- 239000000839 emulsion Substances 0.000 claims abstract description 59
- -1 polyethyleneglycol Chemical class 0.000 claims abstract description 45
- 235000000346 sugar Nutrition 0.000 claims abstract description 23
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 19
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 19
- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 14
- 239000004215 Carbon black (E152) Substances 0.000 claims abstract description 13
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- 150000003077 polyols Chemical class 0.000 claims abstract description 8
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 4
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 73
- 239000010452 phosphate Substances 0.000 claims description 73
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 70
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 52
- 150000003254 radicals Chemical group 0.000 claims description 43
- 239000000203 mixture Substances 0.000 claims description 29
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 26
- 239000004094 surface-active agent Substances 0.000 claims description 23
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- 239000002502 liposome Substances 0.000 claims description 18
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- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 claims description 13
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- SHZGCJCMOBCMKK-UHFFFAOYSA-N 6-methyloxane-2,3,4,5-tetrol Chemical compound CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims description 7
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims description 6
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims description 6
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- 239000003550 marker Substances 0.000 claims description 6
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- 125000001424 substituent group Chemical group 0.000 claims description 6
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- CBXCPBUEXACCNR-UHFFFAOYSA-N tetraethylammonium Chemical compound CC[N+](CC)(CC)CC CBXCPBUEXACCNR-UHFFFAOYSA-N 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 229960004605 timolol Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
- ZMANZCXQSJIPKH-UHFFFAOYSA-O triethylammonium ion Chemical compound CC[NH+](CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-O 0.000 description 1
- 229960003962 trifluridine Drugs 0.000 description 1
- VSQQQLOSPVPRAZ-RRKCRQDMSA-N trifluridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(C(F)(F)F)=C1 VSQQQLOSPVPRAZ-RRKCRQDMSA-N 0.000 description 1
- HDZZVAMISRMYHH-KCGFPETGSA-N tubercidin Chemical compound C1=CC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O HDZZVAMISRMYHH-KCGFPETGSA-N 0.000 description 1
- RULSWEULPANCDV-PIXUTMIVSA-N turanose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](C(=O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RULSWEULPANCDV-PIXUTMIVSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 229960003048 vinblastine Drugs 0.000 description 1
- JXLYSJRDGCGARV-XQKSVPLYSA-N vincaleukoblastine Chemical compound C([C@@H](C[C@]1(C(=O)OC)C=2C(=CC3=C([C@]45[C@H]([C@@]([C@H](OC(C)=O)[C@]6(CC)C=CCN([C@H]56)CC4)(O)C(=O)OC)N3C)C=2)OC)C[C@@](C2)(O)CC)N2CCC2=C1NC1=CC=CC=C21 JXLYSJRDGCGARV-XQKSVPLYSA-N 0.000 description 1
- OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 description 1
- 229960004528 vincristine Drugs 0.000 description 1
- OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 description 1
- HBOMLICNUCNMMY-XLPZGREQSA-N zidovudine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-XLPZGREQSA-N 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K23/00—Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
- C09K23/14—Derivatives of phosphoric acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
- A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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- C07H11/04—Phosphates; Phosphites; Polyphosphates
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- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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Abstract
Perfluoroalkylated amphiphilic phosphorus compounds, corresponding to formulae (Ia), (Ib) wherein V is O or S; R?1, R2 and R3¿ are H or substituted or unsubstituted perfluoroalkylated or hydrocarbon radicals; provided that R?1, R2 or R3¿ is a perfluoroalkylated radical; and Y and Z are radicals which can bear a part derived from a sugar, a polyol, or a hydrophilic polymer such as polyethyleneglycol, a perfluoroalkylated part or a part derived from a pharmaceutically active molecule, and method for their preparation and use. These compounds can be included in preparations, emulsions, dispersions, gels, microemulsions, notably for biomedical uses.
Description
NEW PERFLUOROALKYLATED AMPHIPHILIC PHOSPHORUS
COMPOUNDS:
PREPARATION AND BIOMEDICAL APPLICATIONS
Background of the Invention
Fluorocarbons are preferably used as oxygen carriers, because these compounds are very inert and dissolve high concentrations of gases. However, fluorocarbons must be introduced into the vascular system in a dispersed form, for example as an emulsion, because fluorocarbons are insoluble in water. A surfactant or a combination of surfactants are necessary to achieve a stable fluorocarbon dispersion in water.
One fluorocarbon emulsion in biomedical use is Fluosol-DA 20% or Fluosol, developed by the Green Cross Corp. in Japan. Other more highly concentrated fluorocarbon emulsions are also known, such as those described by C. Long, D. Long, J.G. Riess, R. Follana, A. Burgan and R. Mattrey in "Blood Substitutes", edited by T.M.S. Chang and R.P. Geyer (Marcel Dekker Inc. NY, 1989), pp. 441-442. These fluorocarbon emulsions have certain drawbacks, for the surfactants used are not particularly adapted to the emulsification of fluorocarbons, and do not allow the modulation of the emulsions' characteristics in order to adapt them to specific therapeutic applications. A further, ideal, objective would be the ability to modulate the biological response they trigger in the organism.
Likewise it is desirable to gain further mastery in the art of liposome technology, especially to allow the modulation of the characteristics and properties
of lipid membranes and liposomes and to extend their spectrum of applications, especially for drug and contrast-agent delivery.
Thus, research has been undertaken to find new surfactants and/or cosurfactants which are biocompatible, and better adapted to the emulsification of fluorocarbons than those used at present.
Following this research, it has been found that fluorinated polyhydroxylated compounds and fluorinated derivatives of amino acids could be used in this aim, as described in EP-A- 0 255 443 and EP-A- 0 311 473; but these derivatives are not phosphorus-based compounds.
More recently, amphiphilic molecules containing phosphorus and fluorine have been prepared, as described in WO 90/15807, but the compounds described therein have in their hydrophilic part neither a polyhydroxylated derivative nor a highly polymerized polyethoxylated motif.
It is therefore an object of the invention to develop more effective surfactants specifically suited to providing dispersions or emulsion of fluorocarbons that are particularly suitable for biological use.
Brief Description of the Drawings Figure la sets forth a general synthetic scheme (Method 1) for the preparation of compounds having the general formula Ia and Ib.
Figure lb sets forth a general synthetic scheme (Method 2) for the preparation of compounds having the general formula Ia and Ib.
Figure 2 sets forth a synthetic scheme for the preparation of compounds 3 to 9.
Figure 3a sets forth a synthetic scheme for the preparation of compounds 10-12, 15, and 16.
Figure 3b sets forth a synthetic scheme for the
preparation of compounds 13, 14, and 17-19.
Figure 4 sets forth a synthetic scheme for the preparation of compounds 20 to 23.
Figure 5 demonstrates the ageing at 40°C of perfluorodecalin(PFD) (50% w/v) sterilized emulsions prepared with 3% of surfactant: lecithin, 7a, 7b, or 8b. (Curves of 7b and 8b are superimposable.)
Figure 6 demonstrates the ageing at 40°C of perfluorooctyl bromide(PFOB) (90% w/v) sterilized emulsions prepared with 4% of lecithin or 1% of compound 7b.
Figure 6 demonstrates the ageing at 40°C of perfluorooctyl bromide(PFOB) (90% w/v) sterilized emulsions prepared with 4% of lecithin or 1% of 7b.
Figure 7 demonstrates the ageing at 40°C of PFOB (90% w/v) sterilized emulsions prepared with 4% of lecithin or with lecithin 3.5/0.5% w/v respectively.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides new amphiphilic compounds of phosphorus, containing a highly fluorinated substituent, their preparation procedure and their use, in particular in preparations for biomedical applications. These compounds can be used, in particular as emulsifying agents, in emulsions destined to be used in the biomedical field, for example as oxygen or contrast agents carriers, for intravascular or other types of administration. Another application is their use in the preparation of vesicles or of liposomal formulations as transport and delivery systems of therapeutic active agents.
Among these compositions for biomedical use can be cited those based on fluorocarbons which can be used as blood substitutes, for treating cerebral and cardiac ischemia, in percutaneous transluminal coronary angioplasty, as cardioplegic and reperfusion solutions, as well as in preparations for
sensibilizing tumors to radiation or to chemotherapeutic agents, for the preservation of organs and isolated tissues, for ventriculo- subarachnoid perfusion of the brain and for diagnosis by medical imagery.
The present invention concerns new amphiphilic perfluoroalkylated phosphorus compounds bearing different hydrophilic heads, which can be used in dispersed systems, including fluorocarbon emulsions, and in liposome manufacturing and technology. The choice, in particular, of a hydrophilic head derived from a sugar or a highly polymerized polyethylene glycol moiety connected to a perfluoroalkylated hydrophobic part through a phosphorus-based group, allows increased hydrophilic and amphiphilic character, and modulation of the biological properties and more particularly the biodistribution, intravascular persistence, molecular recognition and tissue targeting of fluorocarbon emulsions and liposome formulations. The phosphate group also allows the introduction of a negative charge simultaneously with the highly fluorinated fragment, the sugar and the polymerized polyethylene glycol moieties. Negatively charged and/or polyethylene glycol embedded liposomes, for example, are known to prolong the intravascular persistence and to modify the biodistribution. The sugar moiety is important in the biodistribution and targeting process, e.g., for the mannose derivative it was observed that alveolar, peritoneal macrophages and blood monocytes of various mammalian species express on the surface of a mannose receptor with high affinity for a specific sugar mannose. This receptor mediates efficient internalization of the perfluoroalkylated amphiphiles, especially when the amphiphiles bear a pharmaceutically active compound. The pharmaceutically active compound will be fixed onto the molecule by a
chemical bond, for example, a covalent bond on the phosphoric group.
According to the invention, the compound corresponds to one of the general formulae:
wherein
1 ) V represents O or S,
2) -X- represents -O-, -S-, or -NR4- with R4 representing a hydrogen atom, a linear or branched hydrocarbon saturated or unsaturated radical, in C1 to C24, or a radical of formula Rf(CH2)a W wherein a is an integer from 0 to 12,
RF is a fluorinated alkylated radical of 2 to 23 carbon atoms, wherein 50 to 100% of the hydrogen atoms have been replaced by fluorine atoms, RF can bear other substituents chosen from among Cl and Br, and RF includes at least 4 fluorine atoms, and
W represents
-(CH=CH)d-(CH2)e-(CH=CH)f-(CH2)g-, where d is an integer from 0 to 12, e is an integer from 0 to 11, f is an integer from 0 to 12, g is an integer from 0 to 11, with d+f=0 to 12, and e+g=0 to 11,
-OCH2CH(CH2OH)CH2-, or
-OCH2CH(CH2OH)-,
3) m is an integer from 1 to 20,
4) R1 represents a radical chosen from among the radicals of formula:
RF RF-(CH2)a-W-A-(CH2)b-,
RF-(CH2CH2O )c-CH2-,
RF-(CH2CH2S)c-CH2-, and
RF-W- wherein
a, RF and W are as defined above,
b is an integer from 1 to 12,
c is an integer from 1 to 12, and
A represents
-O-,
-S-,
-OC(O)-,
C(O)O-
-(R5)N- or -(R5)(R6)N+- with R5 and R6 representing a hydrogen atom, a linear or branched, hydrocarbon radical, saturated or unsaturated in C1 to C24, the hydroxyethyl radical or the RF(CH2)aW radical with RF, a and W such as defined above,
-(CH2)n- wherein n=0 or 1,
-C(O)N(R5)-(CH2)s-B- with R5 such as defined above, s=1 to 12, and B representing -O-, -OC(O)- or -C(O)O-,
-C(O)N(R7)-(CH2)r- or -N(R7)C(O) (CH2)r- with r=1 to 12 and R7 representing a hydrogen atom, a linear or branched hydrocarbon radical, saturated or unsaturated in C1 to C24, the hydroxyethyl radical or the RF(CH2)a-W- radical with RF, W and a such as defined above.
5) R2 represents
a hydrogen atom,
R1-,
RH-W-A-(CH2)b-,
RH-(CH2CH2O)c-CH2-,
RH-(CH(CH3)CH2O)c-CH2-, or
RH-(CH2CH2S)C-CH2-,
with RH representing a hydrogen atom or a linear or branched hydrocarbon chain, saturated
or unsaturated in C1 to C20, and W, A, b and c having the above signification.
6) R3 represents a radical chosen from among the radicals of formulae
RF-,
RF-(CH2)a-W-A-,
RF(CH2CH2O)c-,
RF-(CH(CH3)CH2O)c-,
RF(CH2CH2S)c-,
RF-W-,
RH-W-A-,
RH(CH2CH2O)c-,
RH(CH(CH3)CH2O)c-,
RH(CH2CH2S )c-,
wherein RF, a W, A, c and RH have the above signification, on condition that at least one of R2 and R3 of formula (lb) bears RF part,
7) Y represents a radical chosen from among the radicals of formula:
-X1(CH2)a.X2(R8),
-X1(R8),
wherein a' is an integer from 2 to 12, X1 and X2 independently represent -O-, -S- or -N(R9)- with R9 a hydrogen atom, a linear or branched hydrocarbon radical, saturated or unsaturated, in
C1 to C24, and R8 represents a radical derived from a sugar of the tetrose, pentose, hexose, aminopentose, aminohexose, deoxypentose, deoxyhexose, disaccharide and oligosaccharide series,
from a cyclic hexitol,
from a polyol consisting of the hydrogenated form of a sugar of the tetrose, pentose, hexose, aminopentose, aminohexose, deoxypentose, deoxyhexose, disaccharide and oligosaccharide series,
from the sugars and polyols mentioned
above wherein one or several hydrogen atoms of the OH polyol or sugar groups have been replaced by an acetyl, benzyl, allyl, benzoyl, trityl, isopropylidene, benzylidene, cyclohexylidene group, by a group of formula (CH2CH2O)pR9 with p an integer from 1 to 100 and R9 such as defined above, or by an R1 group such as defined above.
wherein m and X1 are such defined above, R10 and R11 which may be identical or different represent R2 such as defined above, R12 represents R3 such as defined above,
-OH
-OM with M representing an organic or inorganic cation,
-O(CH2CH2O)pR9
-O(CH(CH3 )CH2O)qR9, or
-O(CH2CH2O )g-(CH(CH3)CH2O)q' -(CH2CH2O)q"-R9 wherein R9 is such as defined above, and p, q, q' and q" are integers from 1 to 100, and 8) Z represents a radical chosen from among the radicals of formula:
-X1(CH2)a'-X2(R8),
-X1(R8),
-OH,
-OM,
-O(CH2CH2O)pR9, ·
-O(CH(CH3)CH2O)qR9,
-O(CH2CH2O)q-(CH(CH3)CH2O)q'-(CH2CH2O )q"- R9,
-OCH2CH(OH)CH2OH,
-N(R14)(R15),
-O(CH2)d'-W', and
X2R17
wherein X1, X2, R8, R9, a', p, q, q' and q" are such defined above,
R14 and R15 represent independently a hydrogen atom, a linear or branched alkyl group in C1 to C4, -(CH2CH2O)b,R9, b' is an integer from 1 to 5, or R14 and R15 together form -(CH2)C, with c' an integer from 2 to 5, or R14 and R15 together form with the nitrogen atom a morpholino group
- W' represents an acetic 2-amino group,
-N(R14)(R15), or -N+(R14)(R15)(R16) with R14 and R15 such as defined above, and R16 identical or different with the same definition as R14 or representing a radical derived from an active therapeutic agent, -d' is an integer from 1 to 5, and -R17 is a radical derived from a pharmaceutically active molecule, on condition that
1. Y and Z do not both represent
-OH, -0M or -O(CH2CH2O)pR9 for p=1 to 5,
2. Z does not represent
-OH,
OM,
-N(R14)(R15),
-O-(CH2)d'-W, or
-O-CH2-CH(OH)-CH2OH,
when Y is
3. Z does not represent
-N (R14)(R15),
-O-(CH2), -W', or
-O-CH2-(CHOH)-CH2OH,
when Y represents OH, OM, or O(CH2CH2O)pR9 for p=1 to 5.
In the compounds of formula (Ia) or (lb) of the invention, the presence of a perfluoroalkylated part RF makes is possible to confer on the compound, not only hydrophobic and fluorophilic properties, but also a better biocompatibility, for example avoiding a hemolytic action detectable on human red blood cells, even at concentrations above 50 g/L. The choice of a phosphate-type connector, in the form of a diester, between the fluorophilic part and the sugar-type polar head makes it possible to confer emulsifying properties on the fluorocarbons, which show a distinct improvement over those of the compounds disclosed by EP-A-0-255443. The choice of an intermediate phosphate-type group, in the form of a phosphodiester or phosphotriester, also makes available, at the other end of the compound, strongly hydrophilic groups which can contain, as well, a pharmaceutical substance, an element or a fragment that can serve as marker, fixed by a covalent bond onto this hydrophilic extremity.
Thus the compounds of the invention can be used as vectors of drugs, of active principals or of
markers .
According to the invention, the fluorinated alkyl radicals RF to be used can be chosen from among radicals of formulae:
a) F(CF2)i- wherein i is an integer from 2 to
12,
b) (CF3)2CF-(CF2)j- wherein j is an integer from 0 to 8,
c) RF1(CF2CF(CF3) )k- wherein RF1 represents CF3-, C2F5- or (CF3)2CF- and k is an integer from 1 to 4,
d) (RF2)(RF3)CFO(CF2-CF2)1- wherein RF2 and RF3 represent independently CF3-, C2F5-, n-C3F7- or CF3CF2CF(CF3)-, or RF2 and RF3 together form -(CF2)4or -(CF2)5-, and 1 is an integer from 1 to 6. e) CF3CF2O(CF2CF2O)tCF2- wherein t is an integer from 0 to 5,
f) CF3(CF2)2O(CFCF3)CF2O)u-CF(CF3)- wherein t is an integer from 0 to 6, and
the radicals of paragraphs a. to f. wherein one or several fluorine atoms are replaced by one or several hydrogen, chlorine or bromine atoms, provided that at least 50% of the atoms bound to the RF carbon skeleton be fluorine atoms and RF contain at least 4 fluorine atoms.
In the above general formulae, hydrocarbon radicals apply to radicals derived from saturated, monounsaturated and polyunsaturated hydrocarbons, the unsaturation may be ethylenic or acetylenic, and the radicals either linear or branched.
When in the above formula Y or Z represents - X1( CH2 )a,X2( R8 ) or - X1(R8 ) , with R8 representing a radical derived from a sugar, the sugars derived from the tetrose series can be, for example, erythrose or threose; the sugars of the pentose series can be arabinose, lyxose, ribose, xylose or fructose; the sugars belonging to the hexose series can be allose,
altrose, galactose, glucose, gulose, idose, mannose, or talose; the sugars belonging to the aminopentose or aminohexoses series can be, for example, galactosamine, glucosamine or mannosamine; the sugars belonging to the deoxypentose or deoxyhexose series can be fucose or rhamnose; the sugars belonging to the disaccharide and oligosaccharide series can be for example cellobiose, lactose, maltose, melibiose, palatinose, sucrose, trehalose, turanose, maltotriose and maltotetraose; the hydrogenated forms of the above sugars can be erythritol, threitol, arabinitol, ribitol, xylitol, altritol, galactitol, glucitol, gulitol, iditol,mannitol, galactamine and glucamine, or the polyols belonging to the cyclic hexitol series, for example myo-inositol.
These radicals derived from sugar can be in the form of D or L.
When Y or Z represents OM with M an organic or inorganic cation, this inorganic cation can be chosen from among >NH4 + and metals, particularly pharmaceutically acceptable metals, for example cations Li+, Na+, K+, Mg2+ and Ca2+; the organic cations are particularly quaternary ammonia cations and cations derived from cyclic organic bases, for example triethylammonium, pyridinium, tetraethylammonium, tetrabutylammonium, cyclohexylammonium and dicyclohexylammonium cations.
When Z represents X2R17 with R17 derived from a biologically active molecule, R17 may be, for example, derived from a nucleoside such as thymidine, cytidine, adenosine or uridine, this last bearing, for example, an active substituent, a marker.
A non-restrictive list of examples of such substituents or elements is
- 3'-azido-3'deoxy-thymidine (AZT),
- 3'-difluoro-3'deoxy-thymidine,
- 2'-dideoxy,2'-fluoro-3-ara-adenosine.
ribavirine, cordicepine, cytarabine, 2'- (methylamino)-5-deoxyuridine, psicofuranine, puromycine, thioguanosine, toyocamycine, trifluridine, tubercidine or vidarabine, all of these derivatives being known to chemists (cf Merck Index, 10th edition, 1983).
In the compounds of formula ( Ia ) or ( lb ) described above, V and X represent O to advantage.
According to a suitable preparation method of the invention, one at least of Y and Z is
-X1(CH2)a'X2(R8), or
-X^R8)
-O(CH2CH2O)pCH3 with p = 6 to 100, or - O( CH( CH3 )CH2O )qCH3 with q = 1 to 100.
The advantage of the presence of this type of substituents is that they are biologically well- accepted, and thus improve the biological compatibility of the compounds of the invention.
Preferably, when Y or Z is -X1R8, X1 is -O-; when Y or Z is -X1(CH2)a'X2(R8), X1 and X2 are -O- and a' is equal to 2.
For example, R8 can be a radical derived from glucose, xylitol or mannose.
In the compounds of the invention corresponding to formula (Ia), the following radicals are preferably used for R1:
- RF is a radical of formula CnF2n+1 with n=6 to 8,
- W is the radical (CH=CH)d-(CH2)e with d=0 or 1 and e=0, 1, 2, 4 or 5,
- a is 0, 2, 4 or 5,
- A is -C(O)O- or -O-, and
- b is 1.
For R2, the use of radicals of formula R1 or a hydrogen atom is preferable.
In the compounds of the invention corresponding to formula ( lb), it is generally preferable to use for
R2 the radicals R1 described above or hydrocarbon
radicals of type RH-A-(CH2)b wherein RH is a linear alkyl radical of 1 to 22 carbon atoms, A is -O- or C(O)O-, and b is equal to 1.
In these compounds, R3 is preferably a radical of formula RF-(CH2)a-W-A, RH-A or RF with RF representing
CnF2n+1, where n is an integer from 6 to 8, a is an integer from 4 to 10, A is -0- or - C(O)O-, and RH is a linear alkyl radical from 1 to 22 carbon atoms.
In compounds of formula (Ia) or (lb), radicals of formula OH, OM, X2R17 or radicals with the same formula as Y are preferable for Z. As seen above, Y is preferably:
- X1(CH2)a'X2(R8) or
- X1(R8)
- O(CH2CH2O)pCH3 with p=6 toO 100, or O(CH(CH3)CH2O)qCH3 with q=1 to 100.
Examples of preferred compounds in conformity with the invention are listed in claims 16 to 20.
The compounds of the invention of formula (Ia) or (lb) can be prepared by the following steps, as illustrated in Figure la:
1) Reaction of a compound of formula: or
wherein R1, R2, R3, X and m have the above signification, with the compound of formula VPCl3 wherein V has the above signification, to obtain a compound of formula:
or
2) Reaction of a compound of formula (IIIa) or (IIIb) with a compound of formula HY or HY' and a compound of formula HZ or HZ' wherein Y and Z have above the signification given in Claim 1 and Y' and Z' represent radicals Y and Z bearing hydroxyl groups protected by blocking groups, and
3 ) When a compound of formula HY' or HZ' is used, deprotection of the hydroxl groups of the compound of formula:
or
thus obtained.
In the first step of this procedure, an alcohol, a thiol or a perfluoroalkylated amine is converted into a phosphorodichloridate or thiophosphorodichloridate by phosphorylation with phosphorus oxytrichloride or phosphorus thiotrichloride. This condensation can be achieved in a solvent such as ether, in the presence of triethylamine, with the drop-by-drop addition of the alcohol, thiol or amine solution in dry ether to a solution of VPCl3 in ether to limit the reaction at the monoesterification stage. After elimination of excess VPCl3, the phosphorodichloridate obtained generally contains less than 5% of diester and almost no triester, as this is undetectable.
In the second step of the procedure of the invention, the phosphodiester is prepared by condensation of HY or HY' with phosphorodichloridate, operating in an appropriate solvent such as chloroform, in the presence of triethylamine. During
this reaction, the formation of the triester is difficult to avoid, and thus a- mixture of the triester and the diester is obtained. The phosphodiester can, however, be separated from the mixture in the form of an ammonium salt, and then made to react with an appropriate reactive, HZ or HZ', which allows the introduction of group Z.
When group Y contains hydroxyl groups, the latter, with the exception of that on which the reaction takes place, must be protected by appropriate blocking groups. In this case, then, reactive HY' is used.
Some appropriate blocking groups are acetyl, benzyl, benzoyl, trityl, isopropylidene, benzylidene and cyclohexylidene groups.
The last step of the procedure consists in the deprotection of the hydroxyl groups of Y' to obtain the desired compound. This deprotection can be realized, for example in order to deacetyl, by treatment on a solution of sodium methylate. When it concerns phosphodiester, a compound of formula (Ia) or (lb) wherein Z is ONa is obtained.
In a variant of the procedure of the invention, after the first step of preparation of the phosphorodichloridate, a complementary step of transformation of this phosphorodichloridate into phosphoroditriazolide by reaction with a triazole is performed. This allows the limitation, in the following step, of the formation of the phosphotriester.
Thus, when in the following step a molar ratio of HY'/phosphoroditriazolide 0.5/l is used, only traces of triester are obtained.
Figure la (annexed) shows these two synthetic paths used in the invention.
Compounds of general formula (Ia) or (lb) may also be prepared as shown in Figure lb, through a
procedure including the following steps:
1) Reaction between, respectively, a compound of formula (IIa) or (IIb) and phosphoryltrichloride (PCl3) in the presence of imidazole followed by hydrolysis, leading to a phosphonate compound of formula: or
wherein M is defined as given above;
2 ) Reaction respectively between a compound of formula (IIIc) or (IIId) and an activator (for example pivaloyl chloride, dicyclohexylcarbodiimide, trichloroacetonitrile or arylsulfonyl chloride or arysulfonyl diimidazolide), a compound of formula HY or HY', wherein Y and Y1 are defined as above, leading, after oxidation by I2 or S8, to a compound of formula (I'c) or (I'd):
or
3) Reaction between a compound of formula (I'c) or (I'd), an activator as before, and a compound of formula HZ or HZ', wherein Z and Z' are defined above, followed by a deprotection in the case where a compound of formula HY' or HZ' is used.
The starting products used for this synthesis can be prepared by classical procedures or are available commercially.
The fluorinated derivatives of the invention are of considerable interest for numerous applications because they are strongly amphiphilic. They are, in particular, powerful surfactants, generally soluble or
dispersible in water, biocompatible, for example without any detectable hemolytic action on human red blood cells, sometimes even at concentrations higher than 50 g/L. Moreover, active principles, particularly medicinal drugs, or an element or fragment that can serve as a marker, can be fixed to them by a chemical bond.
The present invention also concerns preparations for biomedical use containing at least one compound of formula (Ia) or (lb) of the invention. These preparations can be in the form of solutions, micelles, dispersions, gels, emulsions and microemulsions in water or any other appropriate solvent. In these preparations, the fluorinated derivative of the invention can play the part of surfactant or cosurfactant, or dispersant, or serve as sa vehicle to solubilize or disperse an active principal in the preparation.
The compounds of the invention can also be used in the pharmaceutical field to prepare medicaments by using them as solvents, dispersants or emulsifiers, in solutions or administrable emulsions, for example for oral administration or by injection.
They can also be used as surfactants in preparations for pharmaceutical, veterinary or phytosanitary use, in preparations for use in biological and medical engineering, comprising in particular fluorocarbon-type oxygen carriers destined, among other uses, to be blood substitutes, or more generally to administer oxygen in vivo, or as fluorocarbon-type contrast agents containing a radiopaque element such as perfluoroctyl bromide (PFOB).
The compounds of the invention wherein Z is X2R17 can also be used as drugs or as markers, for example in preparations where these compounds serve to transport a pharmaceutical compound to an organ, a
cell or a lesion, or to facilitate diagnosis, in particular by radiography, sonography or nuclear magnetic resonance imagery. Indeed, when R17 bears an appropriate marker (fluorescein, a radiopaque or radioactive marker, etc.), the preparations can serve as contrast agents or markers. Being very powerful surfactants, the compounds of the invention can be used in emulsions.
Thus, the invention also concerns an emulsion comprising an oily phase, an aqueous phase and a surfactant constituted by a compound of the invention.
In an emulsion of this type, other surfactants such as lecithins and alkylene oxide copolymers, generally polyoxyethylene and polyoxypropylene can also be included.
In these emulsions, the oily phase can be a hydrocarbon or, preferably, a fluorocarbon or a highly fluorinated compound which can, for example, serve as a carrier of a gas such as oxygen. Fluorocarbons and highly fluorinated compounds can be, for example, linear or cyclic, with molecular weights preferably between 400 and 700, chosen from among the following compounds:
bis(F-alkyl)-1,2-ethenes, particularly bis(F-butyl)-1,2-ethene (F-44E), F-isopropyl-1-F-hexyl-2-ethene and bis(F-hexyl)-1,2-ethene, perfluorodecalin (F-decalin), perfluoromethyldecalins, perfluorodimethyldecalins, perfluoromethyl- and dimethyladamantanes, perfluorodi- and trimethylbicyclo(3,3,1)nonanes and their homologues, ethers of formulae:
(CF3 )2CFO(CF2CF2 )2OCF(CF3 )2, (CF3 )2CFO( CF2CF2 )3OCF(CF3)2, (CF3)2CFO (CF2CF2)2F, (CF3)2CFO(CF2CF2)3F, F(CF(CF3)CF2O)2CHFCF3, F(CF(CF3 )CF2O)3CHFCF3, (C6F13)2O, (C4F9)2O, amines N(C3F7)3, N(C4F9)3, perfluoromethylquinolidines and perfluoromethylisoquinolidines, halogen derivatives C6F13Br, C8F17Br (PFOB), C6F13CBr2CH2Br, 1-bromo 4-
perfluoroisopropyl cyclohexane, C8F16Br2.
These highly fluorinated. compounds can be used alone or in mixtures.
When the oily phase is a fluorocarbon or a highly fluorinated compound, it can represent from 10 to 125% weight/volume of the emulsion.
Generally, the content of the compound of the invention of these emulsions is from 0.01 to 30% in weight/volume when used alone or in association with another surfactant.
The aqueous phase may contain other additives, including inorganic salts, generally in the form of buffers, in order to adjust the pH and the oncotic and osmotic pressures, and to obtain an injectable isotonic composition.
In the preparations comprising the compounds of the invention, the latter can also be incorporated in other dispersed systems, particularly in liposomes.
Thus, the compounds of the invention are also useful in the preparation or modification of lipidic membranes, of liposomes or of niosomes which in turn are usable as drugs or vectors of drugs, also comprising oxygen carriers such as hemoglobin or modified hemoglobin or synthetic chelates. Virtually any active agent (also termed "bioactive agent") can be entrapped within the liposomes for use according to the present invention. Such agents include, but are not limited to, antibacterial compounds such as gentamicin, antiviral compounds such as rifampacin, antifungal compounds such as candicidin anti-parasitic compounds such as antimony derivatives, antineoplastic compounds such as vinblastine, vincristine, mitomycin C, doxorubicin, daunomycin, methotrexate, and cisplatin, among others, proteins such as albumin, toxins such as diptheria toxin, enzymes such as catalase, hormones such as growth hormone, neurotransmitters such as acetylcholine, lipoproteins
such as alpha-lipoprotein, glycoproteins such as hyaluronic acid, immunoglobulins such as IgG, immunomodulators such as the interferons or the interleukins, dyes such as Arsenazo III, radiolabels such as 14C, radio-opaque compounds such as "Te, fluorescent compounds such as carboxy fluoroscein, polysaccharides such as glycogen, cell receptor binding molecules such as estrogen receptor protein, non-steroidal anti-inflammatories such as indomethacin, salicylic acid acetate, ibuprofen, sulindac, piroxicam, and naproxen; antiglaucomic agents such as timolol or pilocarpine, anesthetics such as dibucaine, nucleic acids such as thymine, polynucleotides such as RNA polymers. Also included are various bioactive chemical entities such as peptides, hormones, toxins, enzymes, neurotransmitters, lipoproteins, glycoproteins, immunomodulators, immunoglobulins, polysaccharides, cell receptor binding molecules, nucleic acids, polynucleotides, and the like. Liposomes can also be used in the field of contrast agents and cosmetics.
The procedures for preparing these lipidic membranes, these liposomes or these niosomes are well known to specialists in the field and comprise techniques using solvents, injection, ultrasounds or high-pressure mechanical homogenizers such as a Gaulin homogenizer or a microfluidizer.
Thus, in the present text, the term "dispersed system" may designate dispersions, emulsions, liposomes, niosomes, vesicles, gels, micellar solutions, microemulsinos or other phases of similar structure, containing polar or non-polar substances, including drugs, or an oil, which can be hydrogenated or not and which can contain one or several other surfactants.
In the preparations comprising liposomes, these can be formed from natural or synthetic phospholipids
or lecithins, for example perfluoroalkylated lecithins, or from a mixture of phospholipids and steroids such as cholesterol. In these preparations based on liposomes, the compounds of the invention can be incorporated in a liposomal vehicle and the liposomes can be polymerized.
The preparations, solution, gels, emulsions, dispersions, liposomes and microemulsions of the invention can be used in human and veterinary medicine and in biology, in particular as blood substitutes, contrast agents for diagnosis, media for the treatment of cerebral and cardiac ischemia for perioperative hemodilution, for the preservation of organs, tissues, embryos, or semen, media for therapeutic and cardiovascular surgery, for example as cardioplegic or reperfusion solutions, in coronary angioplasty or as adjuvants in cancer radio- and chemotherapy, and as vectors of medicaments or bioactive agents.
Other characteristics and advantages of the invention will be better seen on reading the following examples, which are of course non-restrictive but given as illustrations with reference to the attached drawing, wherein:
Figure 1, already mentioned, represents schematically four synthetic paths for the compounds of the invention,
- Figures 2, 3 and 4 represent the synthetic schemes corresponding to the examples described,
- Figures 5, 6 and 7 represent the evolution of the average dimensions (in μm) of particles of sterilized emulsions prepared in conformity with the invention as a function of time (in days), during trials of ageing realized at 40ºC.
- Figure 5 concerns emulsions containing 50% w/v of perfluorodecalin (FDC) and 3% w/v of the compound
7a, 7b or 8b; the results with 7b and 8b are merged.
In Figure 5, the results obtained in the same
conditions with a 50% w/v FDC, 3% w/v lecithin emulsion are shown for comparison.
Figures 6 and 7 show, for comparison, the results obtained with an emulsion containing 90% w/v of perfluoroctyl bromide (PFOB) and 4% w/v of lecithin.
Examples 1 to 3 : [2-(F-hexyI)ethyl] (1 ,2,3, 4-tetra-O -acetyl-β- D - glucopyranosyl) (triethylammoπium) phosphate 3a ; [2-(F-hexyl)ethyl] 6-O-(2,3,4- tri-O-acetyl-D-glucopyranosyl) (triethylammonium) phosphate 4a ; [2-(F- hexyl)ethyl] [di-(l,2,3,4-tetra-( O-acetyl-β-D-glucopyτanosyl)] phosphate 5a
Pathway 1: Phosphorylation of 1,2,3,4-tetra-O-acetyl-β-D-gIucopyranose by 1a.
30.6 g of 2-(F-hexyl)ethanol and 17.0 g of triethylamine in ether were allowed to react with 15.4 g of phosphorus oxychloride at 0°C to give 38.7 g of 2-(F-hexyI) ethyl phosphorodichloridate la (96 %).
3.0 g of 1,2,3-4-tetra-O-acetyl-β-D-glucopyranose and 1.7 g of triethylamine in chloroform were added to 4.1 g of la in chloroform. Treatment and silica gel column chromatography gave solid 5a (2.6 g, 55%) and 2.9 g of a mixture in a 95/5 ratio composed of 3a and 4a (ethylammonium salt). Trituration with ether yielded pure solid 3a (2.0 g, 26%).
Pathway 2: Phosphorylation of 1,2,3,4-tetra-O-acetyl-β-D-glucopyranose by 2a.
3.7 g of 1H-1,2,4-triazoIe and 5.5 g of triethylamine were allowed to react with 13.0 g of la in tetrahydrofuran. 4.7 g of 1,2,3,4-tetra-O-acetyI-β-D- glucopyranose in pyridine were added to the solution of 2a. After treatment and filtration through a silica gel column, 10.0 g of a 3a/4a mixture in a 95/5 ratio were obtained. Trituration with ether yielded 3a (7.6 g, 65%).
Analysis of 3a : [α ]D +12.7° (c 1.0, chloroform); 31P-n.m.r. (CDCI3): δ -0.18;
Analysis of 4a : [α ]D +29.0° (c 0.9, chloroform); 31P-n.m.r. (CDCI3): δ - 0.41. Analysis of 5a : [α]D +14.8° (c 1.2, chloroform); 31P-n.m.r. (CDCI3): δ -1.40.
Example 4 : [2-(F-hexyl)ethyl] (6-D-glucosyl) (sodium) phosphate 7a
Compound 3a (5.4 g) was stirred in 1% methanolic sodium methoxide. The solution was brought to pΗ 4 with an Amberlite IR-120 resin (Η+ form), then passed through an Amberlite IR-120 resin column (Na+ form). The resulting solid dissolved in methanol was precipitated by addition of ether to provide solid 7a (3.7 g, 93%).
Analysis of 7a : [α]D +14.8° (c 1.2, water); 3 1P-n.m.r (CD3OD): δ 2.09 and 2.06;
Anal. Calc. for C14H 15F13NaO9P.H2O (646.2) : C, 26.02; H, 2.65; F, 38.22;
Na, 3.55; P, 4.79. Found : C, 26.39; H, 2.71 ; F, 38.70; Na, 3.73; P, 4.80.
Examples 5 and 6 : [2-(F-octyl)ethyl] (1 ,2,3,4-tetra-O -acetyI-β- D glucopyranosyl) (triethylammonium) phosphate 3b ; [2-(F-octyl)ethyl] 6-O-(2,3,4 tri-O-acetyl-D-glucopyranosyl) (triethylammonium) phosphate 4b
13.8 g of 1,2,3,4-tetra-O-acetyl-β-D-glucopyranose in pyridine was added to 2b in tetrahydrofuran prepared as above from 1H-1,2,4-triazole (10.9 g), 1b (45.9 g) and triethylamine (16 g). Similar treatment as above gave 32 g of a 3b/4b mixture (95/5 ratio). Solid 3b (24.9 g, 64%) was obtained after trituration with ether.
Analysis of 3b : [α]D +11.0° (c 1.0, chloroform); 31P-n.m.r. (CDCI3): δ -0.11 Example 7 : [2-(F-octyl)ethyl] (6-D-glucosyl) (sodium) phosphate 7b
After processing as described for 3a, compound 3b (24 g) yielded white solid
7b (16.9 g, 92%). O-Deacetylation of a 3b/4b mixture (1.0 g), as described above gave also pure 7b (0.7 g, 76%).
Analysis of 7b : [α]D +11.8° (c 1.0, water); 3 1P-n.m.r. (CD3OD): δ 2.13 and 2.17; Anal. Calc. for C16Η 15F17NaO9P.Η2O (746.2) : C, 25.75; H, 2.29; F, 43.28 ;
Na. 3.08; P, 4.15. Found : C, 25.96; H, 2.15; F, 44.18; Na, 3.00; P, 4.19.
Example 8 : [2-(F-octyl)ethyl] (1,2:5,6-di-O-isopropylidene-α-D-glucofuranosyl) (triethylammonium) phosphate 6b
6.4 g of 1,2.5,6-di-O-isopropylidene-α-D-glucofuranose in pyridine were added to 2b prepared from 1H-1,2,4-triazole (6.8 g), 1b (28.8 g) and triethylamine (10 g). After treatment and chromatography, 3.2 g of unreacted sugar (50%) was recovered by elution with ether, then elution with chloroform/methanol (80/20) yielded 10.9 g of 6b (triethylammonium salt).
Analysis of 6b : [α]D -17.9° (c 1.2, chloroform); 31P-n.m.r. (CD2CI2): δ -1.24.
Example 9 : [2-(F-octyl)ethyl] (3-D-glucosyl) (sodium) phosphate 8b.
6b (8.8 g, 10 mmol) was dissolved in 90% (v/v) aqueous trifluoroacetic acid .
After treatment, the resulting solid dissolved in a water-meihanol mixture was passed through an Amberlite IR-120 resin column (Na+ form). Lyophilisation of the appropriated fractions led to solid 8b (4.6 g, 65%).
Analysis of 8b : [α]D +14.4° (c 1.1, water); 31P-n.m.r. (CD3OD) : δ 2.82 et 2.66 ;
Anal. Calc. for C16Η 15F17NaO9P.Η2O (746.2): C, 25.75; H, 2.29; F, 43.28; Na, 3.08; P, 4.15. Found : C, 26.20; H, 2.05; F, 43.18; Na, 3.08; P, 4.13.
Example 10 : bis-[2-(F-hexyl)ethyl] 6-(1,2,3,4-tetra-O-acetyl-β-D-glucopyranosyI) phosphate 9a
4.23 g of la were reacted in tetrahydrofuran with 3.06 g of 1,2,3,4-tetra-O- acetyl-β-D-glucopyranose for 19 h, then 3.84 g of 2-(F-hexyl)ethanol were added. After 27 h of reaction, purification by chromatography led to 4.04 g (41 %) of 9a.
Analysis of 9a : [α ]D +8.0° (c 1.1, chloroform); 31P-n.m.r. (CDCI3) : -1-58.
Example 11 : { 1 -[2-(F-octyl)ethyl]decyl } (triethylammonium) hydrogenphosphonate 10
To a solution of imidazole (3.88 g) in toluene at 0°C was added dropwise
PCI3 (1.52 mL) followed by triethylamine (8.4 mL). After 15 mn of stirring, 1-[2- (F-octyl)ethyl]decanol (2.42 g) in toluene was added dropwise. After 90 min, the reaction mixture was quenched with M.TEAB then concentrated. Methylene chloride was added and the organic layer was washed with water and M.TEAB, dried, concentrated and purified on silica gel. Appropriate fractions treated with M TEAB then concentrated yielded 10 (2.11 g, 70%).
Analysis of 10 : 31P-n.m.r. (CDCI3): δ 3.5.
Example 12 : ( 1-[2-(F-octyl)ethyl]decyI) (1,2:3,4-di-O-isopropylidene-α-D-galactopyranosyl) (triethylammonium) phosphate 11
A mixture of 10 (5.91 g) and 1,2:3,4-di-O-isopropylidene-α-D-galactopyranose (2 g) dissolved in pyridine reacted with pivaloyl chloride (2.37 mL). After 15 min, iodide (3.89 g) in pyridine/water (98/2, 19.1 mL) was added.
After 4 hours of stirring, the solution was evaporated, then methylene chloride was added and the organic layer was washed with M Na2S2O3, water and M TEAB, dried, concentrated and purified on a silica gel. Treatment with M TEAB yielded 11
(3.91 g, 50%).
Analysis of 11 : 19F-n.m.r. (CDCI3): δ -81.5 (CF3), -115 (CF2CH2), -122.6-123.8
(10F), -126.8 (CF2CF3).
Example 13 : ( 1-[2-(F-octyl)ethyl]decyl) (6-D-galactosyl) (sodium) phosphate 12
11 (2.9 g, acid form) was stirred in CF3CO2H-H2O (90/10). After 15 min, the solvent was evaporated. The resultant oil dissolved in aqueous methanol was
treated with an Amberlite IR-120 resin (Na+ form). Filtration, concentration a precipitation in acetone led to a solid which was purified by trituration with ether give solid 12 (1.7 g, 70%).
Analysis of 12 : 13C-n.m.r. (CD3OD): δ 100.8 (C-1β), 94.2 (C-1α), 75.4 (d 3J C, P 6.1 Hz, C-5β), 74 (d, 3JC,p 7.3 Hz, CH), 73.7 (C-3β), 72.7 (C-2β), 69.8 (C-5α), 69.4 (C-4α), 69.3 (C-3α), 69.2 (C-4β), 68.6 (C-2α), 64.3 (2d, 2JC,p. 5.5 Hz, C- 6α, C-6β), 36.3 (d, 3JC,P. 5.5 Hz, C-1), 33.1 (C-2), 30.6 (2s, (CH2)4), 28 (t, 2Jc,F, 22 Hz, CH2C8F17), 26.7 (CH2CH2C8F17), 26.1 (C-7), 23.7 (C-8), 1 4.4 (C-9); 31P-n.m.r. (CD3OD): δ 1.4.
Example 14 : { 1-[2-(F-octyl)ethyl)decyl ) (1 ,2,3,4-tetra-O-acetyl-β- D-glucopyranosyl) (triethylammonium) phosphate 13
{ 1-[2-F-octyl)ethyl]decyl) (triethylammonium) hydrogenphosphonate 10 (2.58 g) and 1,2,3,4-tetra-O-acetyl-β-D-glucopyranose (1.17 g) in pyridine reacted with pivaloyl chloride (0.93 mL) and iodine (1.7 g) in pyridine/water (98/2) according to the method described for 11 yielded 13 (1.37 g, 36%).
Analysis of 13 : 31P-n.m.r. (CDCI3): δ -0.45.
Example 15 : ( 1-[2-(F-octyl)ethylJdecyl) (6-D-glucosyI) (sodium) phosphate 14 13 (0.73 g) was stirred for 15 min in 1% methanolic sodium methoxide. The solution was brought to pH 4 with an Amberlite IR-120 resin (H+ form) then treated with an Amberlite IR-120 resin (Na+ form). Concentration and precipitation in acetone led to a solid purified by trituration with ether giving 14 (0.39 g, 70%).
Analysis of 14 : 31P-n.m.r. (CD3OD): δ 2.2.
Example 16 : ( 1-[2-(F-octyl)ethyl]decyl) (1,2:5,6-di-O-isopropyiidene-α-D-glucofuranosyl) (triethylammonium) phosphate 15
10 (4 g, 5.2 rnmol) reacted with 1 ,2:5,6-di-O-isopropylidene-α- D- glucofuranose (1.35 g) according to the method described for 11 yielded 15 (4.3 g, 81 %).
Analysis of 15 : IR (cm- 1) : 3445 (N-C2H5), 2930-2860 (CH), 1210- 1 150 (CF),
1210 (P=0), 1075 (P-O-C).
Example 17 : { 1-[2-(F-octyl)ethyl]decyl) (3-D-glucosyI) (sodium) phosphate 16
15 (4.2 g, 4.84 mmol) treated with the same process as for the compound 11 yielded 16 as a solid (1.78 g, 50%).
Analysis of 16 : 31P-n.m.r. (CD3OD) : δ 3.2 and 2.8.
Example 18 : { 1-[2-(F-hexyl)ethyl]dodecyl } (triethylammonium) hydrogenphosphonate 17
As described for the compound 10, PCI3 (1.88 mL) and [1-2-(F- hexyl)ethyl]dodecanol (3 g) yielded 17 (3.15 g, 80%).
Analysis of 17 : 31P-n.m.r. (CDCI3) : δ 3.9.
Example 19 : { 1-[2-(F-hexyl)ethyl]dodecyl} (1,2,3,4-tetra-O-acetyl-β-D- glucopyranosyl) (triethylammonium) phosphate 18
Compound 17 (0.5 g) reacted with 1,2,3,4-tetra-O-acetyl-β-D - glucopyranose (0.26 g) as described for 11 yielding 18 (0.38 g, 50%).
Example 20 : { 1-[2-(F-hexyl)ethyI]dodecyl) (6-D-glucosyI) (sodium) phosphate 19 18 (3.5 g) treated as the compound 13 yielded a solid (1.6 g, 60%).
Example 21: [2-(F-octyl)ethyl] bis [polyethylene glycol methyl ether] phosphate triester 20, n = 7.
2-(F-octyl)ethanol (7.9 g) and triethylamine (2.9 mL) reacted in ether at 0°C with POCI3 (1.90 mL). After filtration, the excess of POCl3 was distilled. The crude product was-dissolved in ether and polyethylene glycol methyl ether (23.0 g : MW 350) and triethylamine (9.3 mL) were added. After stirring at room temperature for 48 hours and treatment, 11.5 g (56%) of 20 were obtained.
Analysis of 20 : Η-n.m.r. (CDCI3) : δ 2.32 - 2.65 (m, 2H, CF2CH2), 3.31 (s, 6H, OCH3), 3.43 - 3.75 (m, ~ 57 H, CH2O(CH2CH2O)n-1), 4.07 - 4.23 (m, 4H, POCH2), .4.31 (dt, 2H, 3JHp = 7.7Hz, 3J HH = 5.9Hz, CH2OP) ; 31p-n.m.r. (CDCl3) : δ -0.69.
Example 22 : [5-(F-octyl)pentyl] bis [polyethylene glycol methyl ether] phosphate triester 21, n = 7.
The process of example 21 applied first to 5.0 g of 5-(F-octyl)pentanol, 1.7 mL of triethylamine and 1.1 mL of POCI3, then to 12.8 g of polyethylene glycol
methyl ether (MW 350) and 7.0 mL of triethylamine yielded, after treatment, 7.0 g
(57%) of 21.
Analysis of 21 : 31P-n.m.r. (CDCI3) : δ -0.26. Example 23 : [5-(F-octyl)pentyl] bis [polyethylene glycol methyl ether] phosphate triester 22, n = 16.
The process of example 21 applied first to 4.8 g of 5-(F-octyl) pentanol, 1.6 mL of triethylamine and 1.1 mL of POCI3, then to 25.7 g of polyethylene glycol methyl ether (MW 750) and 3.1 mL of triethylamine yielded, after treatment, 10.0 g (51%) of 22.
Analysis of 22 : Η-n.m.r. (CDCI3) : δ 1.31 - 1.72 (m, 6H, (CH2)3), 1-80 - 2.15 (m, 2H, CF2CH2), . 3.28 (s, 6H, OCH3), 3.40 - 3.80 (m, - 130 H, CH2O(CH2CH2O)n-1), 3.80 - 4.16 (m, 6H, CH2OP and POCH2) ; 31P-n.m.r. (CDCl3) : δ -0.19.
Example 24 : [5-(F-octyl)pentyl] [polyethylene glycol methyl ether] phosphate, sodium salt 23, n = 45.
The process of example 21 for the first step was applied to 7.4 g of 5-(F-octyl)pentanol, 2.5 mL of triethylamine and 1.7 mL of POCI3. After filtration and removal of the solvent, the crude reaction product was dissolved in chloroform and
29.4 g of polyethylene glycol methyl ether (MW 2000), 9.5 mL of pyridine and 0.2 g of dimethylaminopyridiήe were added. Stirring for 24 hours and treatment yielded 23 (21 g, 55%).
Analysis of 23 : 1H-n.m.r. (CDCI3) : δ 1.26 - 1.70 (m, 6H, (CH2)3), 1-75 - 2.17 (m, 2H, CF2CH2), 3.30 (s, 3H, OCH3), 3.34 (m, ~ 190 H, CH2OP and
CH2O(CH2CH2O)n-1), 3.93 (m, 2H, POCH2) ;31P-n.m.r. (CDCI3) : δ -3.50.
SURFACE ACTIVITY
The compounds described in this invention are very efficient surface agents, greatly lowering the surface (γs) and interfacial (γj) tensions between water and a fiuorocarbon when added to water. Thus at 1 g/L in water, γs goes from 73 (pure water) to 19.7-33.8 mNm- 1, and γi[water/perfluorodecaIin (FDC) or perfluorooctyl bromide (PFOB)] goes from 56 and 51 (in the absence of surfactant) to 5.1-8.9
mNm-1 and 4.7-10.5 mNm-1 respectively, as illustrated by the examples assembled in the table below.
These compounds are also characterized by low values of critical micellar concentration (CMC: from 1.10-6 to 1.7.10-3 M).
SURFACE ACTIVITΎ DATA OF AQUEOUS SOLUTIONS (20°C, ± 0.3 mNm-1).
Compound MW Dispersibility g/L CMC Concentration γs Ti/FDC -yi/PFOB in water (25°C) (mM) g/L (mM)
H2O 73.0 56.0 51.0
7a 646.2 350 1.55 1 1.548 19.7 5.6 4.7
8b 746.2 250 1.68 1 1.340 27.5 5.6 8.3
20 1208 >100 0.025 0.1 0.083 33.8 13.1 9.2 1 1250 >100 0.001 0.01 0.008 29.6 9.6 7.5
BIOLOGICAL EVALUATION
The biocompatibility of the compounds claimed in the present invention is illustrated in particular by the fact that aqueous solutions at 1 g/L of these compounds do not modify the growth and viability of Namalva cell cultures. The perfluoroalkylated chain of these compounds confers a protective character; thus a dispersion at 30 g/L of compound 7b in a solution at 20 g/L of Pluronic F-68® shows no hemolytic effect, whereas an aqueous solution of its hydrocarbon analogue is very hemolytic even at 5 g/L. Compound 7a shows no hemolytic activity, even at a concentration as high as 100 g/L. It is also characterized by a LD50 on the order of 750 mg/kg in intravenous injection into mice. Compound 22 is non-hemolytic at a 50 g/L concentration, and its LD50 is higher than 2000 mg/kg in intravenous injection into mice. Concerning compounds 21 and 22, which differ by the degree of polymerization of the polyethyleneglycol head, it should be pointed out that their in vivo tolerance increases from 21 to 22, i.e. with increasing degree of
polymerization. Furthermore, these derivatives show a higher i.v. tolerance than the single-chain compounds disclosed in WO 90/15807 C (25 <LD50≤125 mg/kg bw).
EMULSIONS
The products described in this invention, used as surfactants or cosurfactants, allow the preparation and stabilization of fluorocarbon emulsions. The emulsions were prepared by sonification (BRANSON B30 sonicator) (for 50% w/v of fluorocarbon) or by high-pressure microfluidization ((MICROFLUIDICS M 110)) (for more conentrated fiuorocarbon emulsions). The aging of these emulsions at 40°C (accelerated aging conditons) was followed by measuring the average size of the particles by a photosedimentation method (HORIBA CAPA 500) and compared with reference emulsions prepared with natural egg-yolk lecithins or poloxamers, for example Pluronic F-68.
Emulsions prepared with the sonicator
Example 25 : formulation with 7b 3% w/v; perfluorodecalin (FDC ) 50% w/v
To prepare 12 mL of an emulsion of FDC (50% w/v) with 3% w/v of 7b, the surfactant (360 mg) was first sonicated in a rosette cell at 0°C, in 8.55 mL of water. Next, the FDC (6 g) was added and the whole is sonicated, until optimal average particle size and size distribution are obtained. The emulsion was then divided into
aliquots, stocked under inert atmosphere, sterilized for 8 min at 121°C in a autoclave, then stored at 40°C and monitored over time. The results are presented i figure 5. Example 26 : formulation with 7b 1%, lecithin 2% w/v; FDC 50% w/v
The same process but with a mixture of surfactants composed of 120 mg o
7b and 240 mg of natural egg-yolk lecithins (EYP) gave FDC emulsion with th following average particle sizes : 0.15 μm on preparation, 0.21 μm after sterilizatio and 0.32 μm after 30 days at 40°C (respectively 0.19 μm, 0.23 μm and 0.45 μm fo the emulsion prepared with EYP alone).
Example 27 : formulation with 7b 1%, Pluronic F-68 2% w/v; FDC 50% w/v
In the same conditions as in example 25, 120 mg of 7b and 240 mg o
Pluronic F-68 and 6 g of FDC gave an emulsion with an average particle size of 0.29 μm at preparation, 0.29 μm after sterilization and 0.64 μm after 30 days at 40°C
(respectively 0.29 μm at preparation, and after sterilization compared to 0.62 μm forthe emulsion prepared with Pluronic alone).
Emulsions prepared with the microfluidizer
Example 28 : formulation with 7b 1% w/v; perfluorooctyl bromide (perflubron,
PFOB) 90% w/v
To prepare 40 mL of a 90% w/v PFOB emulsion, surfactant 7b (400 mg) was first dispersed in 19.6 mL of a phosphate buffer with an Ultra Turrax. PFOB
(36 g) was then added slowly and the whole was mixed for 10 min at 24000 revolutions/min. The resulting pre-emulsion was transferred into a microfluidizer and emulsified for 1 min at 800-1000 bar. The evolution of the average particle size over time at 40°C are presented in figure 6.
Example 29 : formulation with 7b 0.5%, EYP 3.5% w/v; PFOB 90% w/v
This emulsion was prepared as for example 28 with 200 mg of 7b and 1.4 g of EYP for 35 g of PFOB. The ageing results are presented in figure 7.
The stabilizing effect obtained by employing the compounds of the invention is illustrated in figure 5 : after a month at 40°C, the average particle sizes of the
emulsions obtained are, even after sterilization, lower that those of the reference emulsions based on EYP alone.
The stabilizing power of these compounds is considerable compared to that of EYP, even when they are used in small proportions : thus an emulsion concentrated in fluorocarbon (90% w/v), here PFOB, prepared with only 1% w/v of compound 7b, is more stable than an emulsion containing 4% of EYP (see figure 6).
These compounds also present a strong stabilizing co-surfactant effect with EYP : in small amounts, in association with EYP, they facilitate the emulsification process (shorter emulsification times, smaller particle sizes), as illustrated in figure 7. Thus for a typical emulsion, incorporating 4% of EYP, the emulsification process is facilitated by replacing a minute quantity of the latter (1/8th) by compound 7b; this significantly reduces the average particle size and causes strong stabilization.
Claims
1. A compound corresponding to one of the general formulae:
wherein
V is O or S;
-X- is -O-; -S-; or -NR4-; wherein
R4 is a hydrogen atom; a linear or branched, saturated or unsaturated C1 to C24 hydrocarbon radical; or
RF(CH2)a W; wherein
a is an integer from 0 to 12;
RF is a fluorinated C2 to C23 alkyl radical; wherein 50 to 100% of the hydrogen atoms have been replaced by fluorine atoms, and RF can bear other substituents chosen from among Cl and Br, and RF includes at least 4 fluorine atoms; and
W is
-(CH=CH)d-(CH2)e-(CH=CH)f-(CH2)g-; wherein
d is an integer from 0 to 12;
e is an integer from 0 to 11;
f is an integer from 0 to 12;
g is an integer from 0 to 11;
d+f=0 to 12; and
e+g=0 to 11;
-OCH2CH(CH2OH)CH2-; or
-0CH2CH(CH2OH)-; and
m is an integer from 1 to 20;
R1 represents a radical selected from the group consisting of: RF;
RF-(CH2)a-W-A-(CH2)b-;
RF-(CH2CH20)c-CH2-;
RF-(CH(CH3)CH2O)c-CH2-;
RF-(CH2CH2S)C-CH2-; and
RF-W-; wherein
a, RF and W are as defined above,
b is an integer from 1 to 12;
c is an integer from 1 to 12; and
A represents
-O-;
-S-;
-OC(O)-;
-C(O)O-;
-(R5)N-; or
-(R5)(R6)N+-; wherein
R5 and R6 represent a hydrogen atom; a linear or branched, saturated or unsaturated C1 to C24 hydrocarbon radical; the hydroxyethyl radical; or the RF(CH2)aW radical; wherein
RF, a, and W are as defined above;
-(CH2)n-; wherein n=0 or 1;
-C(O)N(R5)-(CH2)ε-B-, wherein
R5 is as defined above;
s=l to 12; and
B is -O-; -OC(O)- or -C(O)O-;
-C(0)N(R7)-(CH2)r- or -N(R7)C(O) (CH2 )r-; wherein
r=l to 12; and
R7 represents a hydrogen atom; a linear or branched, saturated or unsaturated C1 to C24 hydrocarbon radical;
the hydroxyethyl radical; or
the RF(CH2)a-W- radical; wherein
RF, a, and W are as defined above.
R2 represents
a hydrogen atom; R1-;
RH-W-A-(CH2)b-;
RH-(CH2CH2O)c-CH2-;
RH-(CH(CH3)CH2O)c-CH2-; or
RH-(CH2CH2S)C-CH2-; wherein
RH represents a hydrogen atom; or
a linear or branched, saturated or unsaturated C1 to C20 hydrocarbon chain; and
W, A, b and c are as defined above.
R3 represents a radical selected from the group consisting of:
RF-;
RF-(CH2)a-W-A-;
RF(CH2CH2O)c-;
RF-(CH(CH3)CH2O)c-;
RF(CH2CH2S)c-;
RF-W-;
RH-W-A-;
RH(CH2CH2O)c-;
RH(CH(CH3)CH2O)c-;
RH(CH2CH2S)c-; wherein
RF, a, W, A, c and RH are as defined above; provided that at least one of R2 and R3 of formula (lb) bears the RF part;
Y represents a radical selected from the group consisting of:
-X1(CH2)a.X2(R8); or
-X1(R8); wherein
a' is an integer from 2 to 12;
X1 and X2 are independently -O-, -S- or -N(R9)-; wherein
R9 is a hydrogen atom; a linear or branched, saturated or unsaturated C1 to C24 hydrocarbon; and R8 represents a radical derived from the group consisting of:
a sugar which is a tetrose, pentose, hexose, aminopentose, aminohexose, deoxypentose. deoxyhexose, disaccharide and oligosaccharide; a cyclic hexitol, a polyol consisting of the hydrogenated form of a sugar of the tetrose, pentose, hexose, aminopentose, aminohexose, deoxypentose, deoxyhexose, disaccharide or oligosaccharide series; or a sugar or polyol as defined above; wherein
one or several hydrogen atoms of the OH polyol or sugar groups have been replaced by an acetyl, benzyl, allyl, benzoyl, trityl, isopropylidene, benzylidene, or
cyclohexylidene group; by a group of formula (CH2CH2O)pR9; wherein
p is an integer from 1 to 100; and R9 is as defined above; or
by an R1 group as defined above;
wherein m and X1 are as defined above;
R10 and R11, which may be identical or
different, represent R2 as defined above; and R12 represents R3 as defined above;
-OH; -OM ; wherein M is an organic or inorganic cation;
-O(CH2CH2O)pR9;
-O(CH2(CH3)CH2O)qR9; or
-O(CH2CH2O)q-(CH(CH3)CH2O)q'-(CH2CH2O)q"R9; wherein R9 is as defined above; and
p, q, q' and q" are integers from 1 to 100; and Z represents a radical selected from the group consisting of:
-X1(CH2)a.-X2(R8);
-X1(R8);
-OH;
-OM;
-O(CH2CH2O)pR9;
-O(CH(CH3)CH2O)qR9;
-O(CH2CH2O)q-(CH(CH3)CH2O)q'-(CH2CH2O)q"-R9;
-OCH2CH(OH)CH2OH,
-N(R14)(R15);
-O(CH2)d'-W'; and
X2R17 wherein
X1, X2, R8, R9, a', p, q, q' and q" are as defined above;
R14 and R15 are independently a hydrogen atom; a linear or branched C1 to C4 alkyl group; or (CH2CH2O)b'R9, wherein
b' is an integer from 1 to 5; or
R14 and R15 together form -(CH2)c, wherein c' is an integer from 2 to 5; or
R14 and R15 together form with the nitrogen atom a morpholino group;
W represents an acetic 2-amino group; -N(R14)(R15); or -N+(R14) (R15) (R16) ; wherein
R and R15 are as defined above; and
R16 is independently selected from the same group as R14 or represents a radical derived from an active therapeutic agent;
d' is an integer from 1 to 5; and R17 is a radical derived from a pharmaceutically active molecule;
provided that:
Y and Z do not both represent -OH; -OM or - O(CH2CH2O)pR9 wherein p=1 to 5;
Z does not represent
-OH;
-OM;
-N(R14)(R15);
-O-(CH2)d'-W; or
-OCH2-CH( OH )-CH2OH;
when Y is
Z is not
-N (R14)(R15);
-0-(CH2)d'-W'; or
-O-CH2-CH(OH)-CH2OH;
when Y is OH; OM; or O(CH2CH2O)pR9 wherein p=1 to 5.
2. A compound according to Claim 1, wherein RF is selected from the group consisting of radicals of the formulas:
F(CF2)i-; wherein i is an integer from 2 to 12; (CF3)2CF-(CF2)j-; wherein j is an integer from 0 to 8;
RF1(CF2CF(CF3))k-; wherein RF1 represents CF3-; C2F5- or (CF3)2CF-; and k is an integer from 1 to 4; (RF2) (RF3)CFO(CF2-CF2)1-; wherein
RF2 and RF3 independently represent CF3-; C2F5-; n-C3F7- or CF3CF2CF(CF3)-; or
RF2 and RF3 together form -(CF2)4- or -(CF2)5-; and
1 is an integer from 1 to 6;
CF3CF2O(CF2CF2O)tCF2- wherein
t is an integer from 0 to 5;
CF3(CF2)2O(CF(CF3)CF2O)u-CF(CF3)- wherein
u is an integer from 0 to 6; and
the same radicals wherein one or more fluorine atoms are replaced by one or more hydrogen, chlorine or bromine atoms;
provided that at least 50% of the atoms bound to the RF carbon skeleton are fluorine atoms; and RF contains at least 4 fluorine atoms.
3. A compound according to Claim 1 or 2, wherein either Y or Z is:
-X1(CH2)a'X2(R8) or -X1(R8)
wherein X1, X2, a' and R8 are as defined in Claim 1.
4. A compound according to Claim 3 wherein X1 is - O-.
5. A compound according to Claim 3 wherein X1 and X2 are
-O-.
6. A compound according to either of Claims 3 or 5 wherein a' is 2.
7. A compound according to either of Claims 3 or 5 wherein R8 is a radical derived from glucose, mannose, galactose, sucrose, trehalose, maltose or xylitol.
8. A compound according to any one of Claims 1 through 7 wherein X and V are -O-.
9. A compound of formula (Ia) according to any one of Claims 1 to 3 and 8, wherein -R1 represents RF; -R2 represents a hydrogen atom or an alkyl; -Y represents OR8; and -Z represents OH or OM.
10. A compound of formula (Ia), according to any one of Claims 1 through 8, wherein -R1 represents RF; - R2 represents a hydrogen atom or alkyl; and -Y and Z represent OR8.
11. A compound of formula (Ia), according to any one of Claims 1 through 8, wherein
-R1 represents RF-(CH2)a-W-A-(CH2)b-;
-R2 represents RF-(CH2)a-W-A-(CH2)b_;
-Y represents OR8; and -Z represents OH or OM.
12. A compound of formula (la), according to any one of Claims 1, 2, or 8, wherein
-R1 represents RF-;
-R2 represents a hydrogen atom;
-Y represents O(CH(CH3)CH2O)qR9 or -O(CH2CH2O)pR9; and -Z represents O(CH(CH3)CH2O)qR9 or -O(CH2CH2O)pR9.
13. A compound of formula (Ia), according to any one of Claims 1 through 8, wherein
-R1 represents RF-W-;
-R2 represents a hydrogen atom or alkyl;
-Y represents -OR8 or O(CH2)a'-OR8; and
-Z represents X2R17.
14. A compound of formula (la), according to any of Claims 1 through 8, wherein -R2 represents RF-W-A-CH2; -R3 represents RF-W-A-; -Y represents OR8; and -Z represents OH or OM.
15. A compound of formula (lb), according to any one of Claims 1 through 8, wherein
-R2 represents a hydrogen atom;
-R3 represents RF;
- Y represents ; and
- Z represents OR8.
16. A compound selected from the group consisting of the following in organic or inorganic salt form:
(2-(F-hexyl)ethyl)(6-D-glucosyl)phosphate. (2-(F-octyl)ethyl)(6-D-glucosyl)phosphate.
(5-(F-hexyl)pentyl)(6-D-gϊucosyl)phosphate.
(5-(F-octyl)pentyl)(6-D-glucosyl)phosphate.
(2-(F-hexyl)ethyl)(6-D-galactosyl)phosphate.
(2-(F-octyl)ethyl)(6-D-galactosyl)phosphate.
(5-(F-hexyl)pentyl)(6-D-galactosyl)phosphate.
(5-(F-octyl)pentyl)(6-D-galactosyl)phosphate.
(2-(F-hexyl)ethyl)(6-D-mannosyl)phosphate.
(2-(F-octyl)ethyl)(6-D-mannosyl)phosphate.
(5-(F-hexyl)pentyl)(6-D-mannosyl)phosphate.
(5-(F-octyl)pentyl)(6-D-mannosyl)phosphate.
(11-(F-hexyl)undecyl)(6-D-glucosyl)phosphate.
(11-(F-octyl)undecyl)(6-D-glucosyl)phosphate.
(11-(F-hexyl)undecyl)(6-D-galactosyl)phosphate. (11-(F-octyl)undecyl)(6-D-galactosyl)phosphate. (11-(F-hexyl)undecyl)(6-D-mannosyl)phosphate.
(11-(F-octyl)undecyl)(6-D-mannosyl)phosphate.
(5-(F-hexyl)-4-pentenyl)(6-D-glucosyl)phosphate. (5-(F-octyl)-4-pentenyl)(3-D-glucosyl)phosphate. (5-(F-octyl)-4-pentenyl)(6-D-galactosyl).phosphate. (1-(2-(F-hexyl)ethyl)decyl)(6-D-glucosyl)phosphate. (1-(2-(F-octyl)ethyl)decyl)(6-D-glucosyl)phosphate. (1-(5-(F-hexyl)pentyl)decyl)(6-D-glucosyl) phosphate.
(1-(10-(F-octyl)decyl)decyl)(6-D-glucosyl) phosphate.
(1-(2-(F-hexyl)ethyl)decyl)(6-D-galactosyl) phosphate.
(1-(2-(F-octyl)ethyl)decyl)(6-D-galactosyl) phosphate.
(1-(5-(F-hexyl)pentyl)decyl)(6-D-galactosyl) phosphate.
(1-(10-(F-octyl)decyl)decyl)(6-D-galactosyl) phosphate.
(1-(2-(F-hexyl)ethyl)decyl)(6-D-mannosyl)phosphate. (1-(2-(F-octyl)ethyl)decyl)(6-D-mannosyl)phosphate. (1-(5-(F-hexyl)pentyl)decyl)(6-D-mannosyl) phosphate.
(1-(10-(F-octyl)decyl)decyl)(6-D-mannosyl) phosphate.
(1-(2-(F-hexyl)ethyl)dodecyl)(6-D-glucosyl) phosphate.
(1-(2-(F-octyl)ethyl)dodecyl)(6-D-glucosyl) phosphate.
(1-(2-(F-octyl)ethyl)tetradecyl)(6-D-glucosyl) phosphate.
( 1-( 2-(F-octyl)ethyl )tetradecyl ) ( 6-D galactosyl)phosphate.
(2-(F-hexyl)ethyl)(3-D-glucosyl)phosphate.
(2-(F-octyl)ethyl)(3-D-glucosyl)phosphate.
(1-(2-(F-octyl)ethyl)decyl)(3-D-glucosyl) phosphate. 1-(2-(F-octyl)ethyl)dodecyl)(3-D-glucosyl) phosphate.
1,3-di-O-(3-(F-octyl)propanoyl)-2-glyceryl)(6-D-glucosyl)phosphate.
(1,3-di-O-(5-(F-octyl)pentanoyl)-2-glyceryl)(6-D-glucosyl)phosphate.
(1,3-di-O-(5-(F-octyl)pentyl)-2-glyceryl)(6-D-glucosyl) phosphate.
(1,3-di-O-(5-(F-octyl)pentyl)-2-glyceryl)(3-D-glucosyl) phosphate.
(2-(F-octyl)ethyl)(5-xylityl)phosphate.
17. A compound selected from the group consisting of:
(2-(F-octyl)ethyl)bis(polyethylene glycol methyl ether) phosphate, n=7.
(5-(F-octyl)pentyl)bis(polyethylene glycol methyl ether) phosphate, n=7.
(5-(F-octyl)pentyl)bis(polyethylene glycol methyl ether) phosphate, n=16.
(5-(F-octyl)pentyl(polyethylene glycol methyl ether)phosphate, n=45.
(2-(F-octyl)ethyl)bis(polyethylene glycol methyl ether) phosphate, n=45.
(5-(F-octyl)pentyl)(polyethylene glycol methyl ether)phosphate, n=45; in organic or inorganic salt form.
18. A compound selected from the group consisting of:
(3-(F-octyl)-2-propenyl)(6-D-glucosyl)(3-azido-3- deoxy-5-thymidinyl)phosphate.
(2-(F-octyl)ethyl)(6-D-glucosyl)(3-azido-3-deoxy-5- thymidinyl)phosphate.
(2-(F-hexyl)ethyl)(6-D-mannosyl)(3-azido-3-deoxy-5- thymidinyl)phosphate.
(5-(F-octyl)pentyl)(6-D-mannosyl)(3-azido-3-deoxy-5- thymidinyl)phosphate.
( 5-(F-octyl)-4-pentenyl) (2-(α-D- mannopyranosidyl)ethyl) (3-azido-3-deoxy-5- thymidinyl)phosphate.
19. A compound selected from the group consisting of the following in organic or inorganic salt form:
(2,3-di-O-(3-(F-hexyl)propanoyl)-1-glyceryl)(6-D- glucosyl) sodium phosphate.
(2,3-di-O-(5-(F-octyl)pentanoyl)-1-glyceryl)(6-D- glucosyl) phosphate.
(2,3-di-(5-(F-octyl)pentyl)-1-glyceryl)(6-D-glucosyl) phosphate.
(2,3-di-(5-(F-hexyl)pentyl)-1-glyceryl)(3-D-glucosyl)phosphate.
(3-O-(5F-octyl)pentyl)-2-0-dodecyl)-1-glyceryl)(6-D-glucosyl)phosphate.
(2-(10-(F-octyl)decyl)-3-0-pentadecyl)-1-glyceryl) (6-D-glucosyl)phosphate.
20. A compound selected from the group consisting of:
bis(2-(F-hexyl)ethyl)(6-D-glucosyl) phosphate. bis(2-(F-octyl)ethyl)(6-D-glucosyl) phosphate. bis(2-(F-octyl)ethyl)(3-D-glucosyl) phosphate. bis(5-(F-octyl)pentyl)(6-D-galactosyl) phosphate.
21. A composition comprising at least one compound according to any one of Claims 1 through 20.
22. A composition for biomedical use, in particular for therapeutic medical use, or an element or fragment able to serve as a marker, comprising at least one compound according to any one of Claims 1 through 15, wherein Z contains a biologically active substance, .
23. A composition containing at least one compound according to any one of Claims 1 through 20 in incorporated form.
24. A composition containing at least one compound according to any one of Claims 1 through 20 incorporated in natural or synthetic vesicles and liposomes.
25. A composition containing one or more compounds according to any one of Claims 1 through 20 incorporated in vesicles or liposomes based on perfluoroalkylated phospholipids.
26. A composition containing one or more compounds according to any one of Claims 1 through 20 in a vesicular or liposomal form.
27. A composition according to Claim 23 which carries an active principal.
28. An emulsion of a fluorocarbon or another highly fluorinated compound containing at least one compound according to any one of Claims 1 through 20.
29. An emulsion containing an oily phase, an aqueous phase and one or more surfactants constituted by one or more compounds according to any one of Claims 1 through 20.
30. An emulsion according to Claim 29 where the oily phase is a highly fluorinated or perfluorinated compound.
31. An emulsion according to Claim 30, characterized in that it contains one or more other surfactants.
32. An emulsion according to Claim 31, characterized in that the other surfactant is a lecithin or a phospholipid, or a mixture of lecithins or phospholipids, or a copolymer of polyoxyethylene and polyoxypropylene.
33. An emulsion according to Claim 29, 30, 31 or 32, wherein the oily phase is a fluorocarbon chosen from the group consisting of bis-1,2-(F-alkyl)ethenes, and more particularly bis-1,2-(F-butyl)ethene (F-44E), 1-F-isopropyl-2-F-(hexyl)-ethene and bis-1,2-(F-hexyl)-ethene, perfluorodecalin (F- decalin), perfluoromethyldecalins, perfluorodimethyldecalins, perfluoromethyl- and dimethyladamantanes, perfluorodi- and trimethylbicyclo(3,3,l)nonanes and their homologues, ethers of formula:
(CF3)2CFO(CF2CF2)2OCF(CF3)2, (CF3)2CFO(CF2CF2)3OCF(CF3)2, (CF3)2CFO(CF2CF2)2F, (CF3)2CFO(CF2CF2)3F, F(CF(CF3)CF2O)2CHFCF3, F(CF(CF3)CF2O)3CHFCF3, (C6F13)2O, (C4F9)2O, amines N(C3F7)3, N(C4F9)3, perfluoromethylquinolidines and perfluoromethylisoquinolidines, the halogen derivatives C6F13Br, C8F17Br (PFOB), C6F13CBr2CH2Br, 1- bromo 4-perfluoroisopropyl cyclohexane, -C8F16Br2.
34. An emulsion according to any one of claims 29 to 33 comprising from about 0.01 to 30% w/v of the compound and from about 10 to 125% w/v of oily phase.
35. A composition comprising the emulsion of any one of Claims 29 through 34 and/or a compound according to any one of Claims 1 through 20, for modifying the intravascular persistence, the biodistribution and/or the recognition of particles constituting dispersed systems such as emulsions, microemulsions, and liposomes, and the specific cell tissue targeting ot these particles.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9106798A FR2677360B1 (en) | 1991-06-05 | 1991-06-05 | PERFLUOROALKYL PHPHOSPHORUS AMPHIPHILIC COMPOUNDS, THEIR PREPARATIONS AND THEIR APPLICATIONS, PARTICULARLY IN THE BIOMEDICAL FIELD. |
FR91/06798 | 1991-06-05 |
Publications (1)
Publication Number | Publication Date |
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WO1992021688A1 true WO1992021688A1 (en) | 1992-12-10 |
Family
ID=9413494
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1992/001329 WO1992021688A1 (en) | 1991-06-05 | 1992-06-05 | New perfluoroalkylated amphiphilic phosphorus compounds: preparation and biomedical applications |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2018092A (en) |
FR (1) | FR2677360B1 (en) |
WO (1) | WO1992021688A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1305322A2 (en) * | 2000-08-03 | 2003-05-02 | D-Pharm Ltd. | Derivatives of branched-chain lipophilic molecules and uses thereof |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5980936A (en) * | 1997-08-07 | 1999-11-09 | Alliance Pharmaceutical Corp. | Multiple emulsions comprising a hydrophobic continuous phase |
FR2849025B1 (en) * | 2002-12-20 | 2005-10-14 | Rhodia Chimie Sa | DIFLUOROMETHYLENE SUBSTITUTED ALLYL ESTERS, PROCESS FOR THEIR SYNTHESIS AND USE THEREOF |
Citations (4)
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DE2405042A1 (en) * | 1974-02-02 | 1975-08-14 | Hoechst Ag | Mixed fluorinated oxyalkylated alkyl phosphate esters - for oil-repellant finishes on paper |
EP0255443A1 (en) * | 1986-07-29 | 1988-02-03 | Atta | New polyhydroxylated and highly fluorinated compounds, their preparation and their use as surfactants |
EP0311473A1 (en) * | 1987-09-16 | 1989-04-12 | Applications Et Transferts De Technologies Avancees Atta | Fluorinated derivate of amino acids, useful as tensioactive or cotensioactive agents, and preparations for biomedical use containing these derivatives |
WO1990015807A1 (en) * | 1989-06-22 | 1990-12-27 | Applications Et Transferts De Technologies Avancées | Fluorine and phosphorous-containing amphiphilic molecules with surfactant properties |
-
1991
- 1991-06-05 FR FR9106798A patent/FR2677360B1/en not_active Expired - Fee Related
-
1992
- 1992-06-05 AU AU20180/92A patent/AU2018092A/en not_active Abandoned
- 1992-06-05 WO PCT/EP1992/001329 patent/WO1992021688A1/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2405042A1 (en) * | 1974-02-02 | 1975-08-14 | Hoechst Ag | Mixed fluorinated oxyalkylated alkyl phosphate esters - for oil-repellant finishes on paper |
EP0255443A1 (en) * | 1986-07-29 | 1988-02-03 | Atta | New polyhydroxylated and highly fluorinated compounds, their preparation and their use as surfactants |
EP0311473A1 (en) * | 1987-09-16 | 1989-04-12 | Applications Et Transferts De Technologies Avancees Atta | Fluorinated derivate of amino acids, useful as tensioactive or cotensioactive agents, and preparations for biomedical use containing these derivatives |
WO1990015807A1 (en) * | 1989-06-22 | 1990-12-27 | Applications Et Transferts De Technologies Avancées | Fluorine and phosphorous-containing amphiphilic molecules with surfactant properties |
Non-Patent Citations (4)
Title |
---|
CHEMICAL ABSTRACTS, vol. 109, no. 1, 4 July 1988, Columbus, Ohio, US; abstract no. 6733G, J.WAKATSUKI: 'Preparation of Fluoroalkyl Phosphate Esters as Syntheic Liposomes' page 639 ;column 2 ; * |
CHEMICAL ABSTRACTS, vol. 110, no. 17, 24 April 1989, Columbus, Ohio, US; abstract no. 154749C, J.GU ET AL.: 'Synthesis of Fluorocarbon Phospholipids and the Formation of their Liposomes' page 767 ;column 2 ; * |
CHEMICAL ABSTRACTS, vol. 113, no. 25, 17 December 1990, Columbus, Ohio, US; abstract no. 232139G, K.OGAWA ET AL.: 'The Reaction of Epoxy Compounds with Phosphates. I. Synthesis and Physicochemical Properties of the Amphiphilic Phosphate Salt Monomers Sodium Alkyl 2-Hydroxy--3-Methacryloyloxypropyl Phosphate (Cn-AHMP-Na)' page 4 ;column 2 ; * |
CHEMICAL ABSTRACTS, vol. 115, no. 24, 9 December 1991, Columbus, Ohio, US; abstract no. 256478J, C.SANTAELLA ET AL.: 'New Perfluoroalkylated Phospholipids as Injectable Surfactants: Synthesis, Preliminary Physicochemical and Biocompatability Data' page 901 ;column 1 ; * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1305322A2 (en) * | 2000-08-03 | 2003-05-02 | D-Pharm Ltd. | Derivatives of branched-chain lipophilic molecules and uses thereof |
EP1305322A4 (en) * | 2000-08-03 | 2005-04-13 | Dpharm Ltd | Derivatives of branched-chain lipophilic molecules and uses thereof |
US7186703B2 (en) | 2000-08-03 | 2007-03-06 | D-Pharm Ltd. | Derivatives of branched-chain lipophilic molecules and uses thereof |
US7687483B2 (en) | 2000-08-03 | 2010-03-30 | D-Pharm Ltd. | Derivatives of branch-chain lipophilic molecular and uses thereof |
Also Published As
Publication number | Publication date |
---|---|
FR2677360A1 (en) | 1992-12-11 |
FR2677360B1 (en) | 1995-04-14 |
AU2018092A (en) | 1993-01-08 |
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