WO1993007254A1 - Microculture slide ready for use - Google Patents

Microculture slide ready for use Download PDF

Info

Publication number
WO1993007254A1
WO1993007254A1 PCT/IT1992/000121 IT9200121W WO9307254A1 WO 1993007254 A1 WO1993007254 A1 WO 1993007254A1 IT 9200121 W IT9200121 W IT 9200121W WO 9307254 A1 WO9307254 A1 WO 9307254A1
Authority
WO
WIPO (PCT)
Prior art keywords
slide
microculture
receptacles
bowl
microscope
Prior art date
Application number
PCT/IT1992/000121
Other languages
French (fr)
Inventor
Antonio Giorgio
Original Assignee
Istituto Di Ricerche Biologiche F. Redi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istituto Di Ricerche Biologiche F. Redi filed Critical Istituto Di Ricerche Biologiche F. Redi
Publication of WO1993007254A1 publication Critical patent/WO1993007254A1/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

Definitions

  • the colony of the microorganism is taken by a handle of platinum or adhesive tape, placed onto another slide, and brought to the microscope for the
  • the present industrial invention seeks to provide a slide shaped like a multiple little bowl so as to enclose the culture ground and provided with a cap so that it can be 15 allowed to rest and then placed under the eye-piece of the microscope.
  • Figs. 1 and 2 show a plan and a section view of the object of the invention, respectively;
  • Fig. 3 shows an axonometric view of the slide.
  • the slide of the invention is formed of a little bowl, preferably a two-receptacle bowl, including a base 1 carrying the receptacles 2 and 3 provided with a knurled bottom and side walls 4 delimiting the base surface and the height of the bowl so as to form a reservoir.
  • a cap 5 covering the receptacles but not the residual base portion 6 serving as handle to carry the slide can be applied to the such reservoir.
  • the material of the bowls is transparent and the height of the same is designed so as to allow the bowls to be directly focused by the microscope.
  • the culture ground can be in advance placed in both receptacles.
  • the analyst will smear the product to be analyzed and then cover the bowl with the cap, thus allowing the microculture to grow.
  • the two receptacles plus the cap have a thickness (height) which is compatible with the focusing of the microscope so that the colonies growing in the feeding ground contained in the receptacles can be observed with large magnification.
  • both receptacles has a number of protuberances so that the ground cannot slip off upon smearing.
  • the analyst should not uncover the slide so that there is no danger that the microorganisms can spread over the en iron en .
  • the construction made of two separate receptacles allows two different culture grounds, the first selective the second elective, to be prepared.

Abstract

A microculture slide is disclosed which preferably consists of two cells adapted to contain the culture ground and be directly placed under the eye-piece of a microscope.

Description

Microculture slide ready for use
Analysis requiring the development of a microculture have been hitherto carried out by using flat slides coated by the analyst with the culture ground and enclosed in a container all the time needed by the microorganisms to
05 grow at the most suitable temperature after the product to be analyzed has been smeared thereon.
After such lapse of time the colony of the microorganism is taken by a handle of platinum or adhesive tape, placed onto another slide, and brought to the microscope for the
10 readout.
The present industrial invention seeks to provide a slide shaped like a multiple little bowl so as to enclose the culture ground and provided with a cap so that it can be 15 allowed to rest and then placed under the eye-piece of the microscope.
The slide of the invention is shown in the accompanying drawings according to an illustrative, non-limitative 20 embodiment.
Figs. 1 and 2 show a plan and a section view of the object of the invention, respectively;
25 Fig. 3 shows an axonometric view of the slide.
•f
~ With reference to the drawings the slide of the invention is formed of a little bowl, preferably a two-receptacle bowl, including a base 1 carrying the receptacles 2 and 3 provided with a knurled bottom and side walls 4 delimiting the base surface and the height of the bowl so as to form a reservoir.
A cap 5 covering the receptacles but not the residual base portion 6 serving as handle to carry the slide can be applied to the such reservoir.
The material of the bowls is transparent and the height of the same is designed so as to allow the bowls to be directly focused by the microscope.
By using the slide according to the above description the culture ground can be in advance placed in both receptacles. The analyst will smear the product to be analyzed and then cover the bowl with the cap, thus allowing the microculture to grow.
For the analysis the slide will be directly brought under the microscope without any further handling and with the cap still applied thereon.
The two receptacles plus the cap have a thickness (height) which is compatible with the focusing of the microscope so that the colonies growing in the feeding ground contained in the receptacles can be observed with large magnification.
The bottom of both receptacles has a number of protuberances so that the ground cannot slip off upon smearing.
The analyst should not uncover the slide so that there is no danger that the microorganisms can spread over the en iron en .
The construction made of two separate receptacles allows two different culture grounds, the first selective the second elective, to be prepared.
Shape and construction modifications of the present invention -in particular the bowls can be single or multiple- can be made without departing from the scope of the invention as summarized in the following claims.

Claims

Claims
1. A microculture slide wherein it comprises a bowl and a cap which can be applied thereon.
2. The slide of the preceding claim, wherein it can be formed by only one bowl.
3. The slide of the preceding claims, wherein the bowl can be divided in two or more receptacles.
« The slide of the preceding claims, wherein the height of the receptacles and the thickness of the covering cap are such as to allow the product contained therein to be focused by the microscope.
5- The slide of the preceding claims, wherein the bottom is knurled in order to retain the culture ground.
6. The slide of the preceding claims, wherein it has a side base portion serving as handle to carry the slide.
7. The slide of the preceding claims, wherein the construction material is transparent.
PCT/IT1992/000121 1991-10-04 1992-10-02 Microculture slide ready for use WO1993007254A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000037A ITNA910037A1 (en) 1991-10-04 1991-10-04 MICROCULTURE SLIDE READY TO USE.
ITNA91A000037 1991-10-04

Publications (1)

Publication Number Publication Date
WO1993007254A1 true WO1993007254A1 (en) 1993-04-15

Family

ID=11387405

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT1992/000121 WO1993007254A1 (en) 1991-10-04 1992-10-02 Microculture slide ready for use

Country Status (3)

Country Link
AU (1) AU2865892A (en)
IT (1) ITNA910037A1 (en)
WO (1) WO1993007254A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0675195A1 (en) * 1994-03-28 1995-10-04 TAPPITAL Srl Method to fill slides with culture media and slides with culture media obtained with this method
WO1995031529A1 (en) * 1994-05-12 1995-11-23 Biomed Diagnostics, Inc. Biological culture growth and observation device
DE4443902C1 (en) * 1994-12-01 1996-04-18 Will Prof Dr Minuth Chamber for long term cell culture
GB2317694A (en) * 1996-09-27 1998-04-01 Valentine Walsh Device for holding and examining samples
EP1015914A2 (en) * 1997-09-04 2000-07-05 Andrew E. Lorincz Microscope slide
WO2007065711A1 (en) * 2005-12-09 2007-06-14 Europäisches Laboratorium für Molekularbiologie (EMBL) Miscroscope specimen holder
KR101464046B1 (en) * 2012-12-04 2014-11-20 연세대학교 산학협력단 perpendicular slide chamber for three dimensional cell culturing

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU236920A1 (en) * Г. В. Гонений , В. П. Мельниченко REVERSIBLE FRICTION PROTECTION COUPLINTS BY G. V. GONSKOGO AND V. P. MELNICHENKO
FR2602241A1 (en) * 1986-07-30 1988-02-05 Jouan Plate for the microscopic examination of living cell cultures

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU236920A1 (en) * Г. В. Гонений , В. П. Мельниченко REVERSIBLE FRICTION PROTECTION COUPLINTS BY G. V. GONSKOGO AND V. P. MELNICHENKO
FR2602241A1 (en) * 1986-07-30 1988-02-05 Jouan Plate for the microscopic examination of living cell cultures

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Week 42, 30 December 1983 Derwent Publications Ltd., London, GB; AN 83-793537 & SU,A,236 920 (KHARK UNIV) 15 December 1982 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0675195A1 (en) * 1994-03-28 1995-10-04 TAPPITAL Srl Method to fill slides with culture media and slides with culture media obtained with this method
WO1995031529A1 (en) * 1994-05-12 1995-11-23 Biomed Diagnostics, Inc. Biological culture growth and observation device
DE4443902C1 (en) * 1994-12-01 1996-04-18 Will Prof Dr Minuth Chamber for long term cell culture
GB2317694A (en) * 1996-09-27 1998-04-01 Valentine Walsh Device for holding and examining samples
GB2317694B (en) * 1996-09-27 2000-12-20 Valentine Walsh A mould
EP1015914A2 (en) * 1997-09-04 2000-07-05 Andrew E. Lorincz Microscope slide
EP1015914A4 (en) * 1997-09-04 2007-05-02 Andrew E Lorincz Microscope slide
WO2007065711A1 (en) * 2005-12-09 2007-06-14 Europäisches Laboratorium für Molekularbiologie (EMBL) Miscroscope specimen holder
KR101464046B1 (en) * 2012-12-04 2014-11-20 연세대학교 산학협력단 perpendicular slide chamber for three dimensional cell culturing

Also Published As

Publication number Publication date
AU2865892A (en) 1993-05-03
ITNA910037A0 (en) 1991-10-04
ITNA910037A1 (en) 1993-04-05

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