WO1993011660A3 - Maturation, desiccation and encapsulation of gymnosperm somatic embryos - Google Patents

Maturation, desiccation and encapsulation of gymnosperm somatic embryos Download PDF

Info

Publication number
WO1993011660A3
WO1993011660A3 PCT/CA1992/000549 CA9200549W WO9311660A3 WO 1993011660 A3 WO1993011660 A3 WO 1993011660A3 CA 9200549 W CA9200549 W CA 9200549W WO 9311660 A3 WO9311660 A3 WO 9311660A3
Authority
WO
WIPO (PCT)
Prior art keywords
desiccation
maturation
encapsulation
embryos
somatic embryos
Prior art date
Application number
PCT/CA1992/000549
Other languages
French (fr)
Other versions
WO1993011660A2 (en
Inventor
Stephen M Attree
Lawrence C Fowke
Original Assignee
Univ Saskatchewan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Saskatchewan filed Critical Univ Saskatchewan
Priority to AU31541/93A priority Critical patent/AU679435B2/en
Priority to CA002125410A priority patent/CA2125410C/en
Priority to AT93900142T priority patent/ATE202261T1/en
Priority to EP93900142A priority patent/EP0618766B1/en
Priority to US08/244,725 priority patent/US5985667A/en
Priority to DE69231890T priority patent/DE69231890T2/en
Publication of WO1993011660A2 publication Critical patent/WO1993011660A2/en
Publication of WO1993011660A3 publication Critical patent/WO1993011660A3/en
Priority to FI942909A priority patent/FI942909A/en
Priority to NO942324A priority patent/NO942324D0/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H7/00—Gymnosperms, e.g. conifers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds

Abstract

Conifer somatic embryos can be desiccated to a moisture content below 50%. A moisture content as low as 10% can be produced. The desiccated somatic conifer embryos have greatly increased ability to store lipids and other nutrients as compared to the corresponding zygotic embryo. The embryos are desiccated using a method which requires the utilization of ABA and PEG.
PCT/CA1992/000549 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos WO1993011660A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU31541/93A AU679435B2 (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos
CA002125410A CA2125410C (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos
AT93900142T ATE202261T1 (en) 1991-12-19 1992-12-18 DESICCATION OF GYMNOSPERM SOMATIC EMBRYOS
EP93900142A EP0618766B1 (en) 1991-12-19 1992-12-18 Maturation and desiccation of gymnosperm somatic embryos
US08/244,725 US5985667A (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos
DE69231890T DE69231890T2 (en) 1991-12-19 1992-12-18 DESICCATION OF GYMNOSPERM SOMATIC EMBRYONS
FI942909A FI942909A (en) 1991-12-19 1994-06-17 Maturation, drying and encapsulation of somatic embryos in gymnosperm
NO942324A NO942324D0 (en) 1991-12-19 1994-06-17 Maturation, drying and encapsulation of somatic gynosperm embryos

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81017191A 1991-12-19 1991-12-19
US810,171 1991-12-19

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US81017191A Continuation-In-Part 1991-12-19 1991-12-19
US08/159,693 Continuation-In-Part US5464769A (en) 1991-12-19 1993-12-01 Desiccated conifer somatic embryos

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US08/985,788 Continuation US6340594B1 (en) 1991-12-19 1997-12-05 Production of desiccation-tolerant gymnosperm embryos
US08/985,894 Continuation US6372496B1 (en) 1991-12-19 1997-12-05 Desiccation-tolerant gymnosperm embryos

Publications (2)

Publication Number Publication Date
WO1993011660A2 WO1993011660A2 (en) 1993-06-24
WO1993011660A3 true WO1993011660A3 (en) 1993-09-16

Family

ID=25203192

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1992/000549 WO1993011660A2 (en) 1991-12-19 1992-12-18 Maturation, desiccation and encapsulation of gymnosperm somatic embryos

Country Status (10)

Country Link
US (3) US5985667A (en)
EP (1) EP0618766B1 (en)
AT (1) ATE202261T1 (en)
AU (1) AU679435B2 (en)
CA (1) CA2125410C (en)
DE (1) DE69231890T2 (en)
FI (1) FI942909A (en)
NO (1) NO942324D0 (en)
NZ (1) NZ246137A (en)
WO (1) WO1993011660A2 (en)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427593A (en) * 1990-10-26 1995-06-27 Weyerhaeuser Company Analogs of botanic seed
FR2717656B1 (en) * 1994-03-25 1996-06-21 Philippe Boxus Process for the preparation of somatic embryos and vegetative points of plants and its applications.
NZ272210A (en) 1995-05-25 1997-10-24 Carter Holt Harvey Ltd Treatment of somatic embryos to provide viable embryos after storage periods
US20050158699A1 (en) * 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
US5965438A (en) * 1995-06-07 1999-10-12 Phyton, Inc. Cryopreservation of plant cells
JPH09121617A (en) * 1995-10-30 1997-05-13 Yazaki Corp Germination promoter and sowing of plant seed
US5861360A (en) * 1995-12-21 1999-01-19 Basf Corporation Encapsulated plant growth regulator formulations and applications
WO1997023133A1 (en) * 1995-12-21 1997-07-03 Basf Corporation Low rate application of inhibitors of ethylene biosynthesis or action
JP2000510438A (en) * 1995-12-21 2000-08-15 ビー・エイ・エス・エフ、コーポレーション Aminoethoxyvinylglycine in combination with mepicurate chloride
AU1348297A (en) * 1995-12-21 1997-07-14 Basf Corporation Enhancing the rate of seed germination with application of ethylene biosynthesis inhibitors
US6255250B1 (en) 1996-12-20 2001-07-03 Basf Corporation Plant growth regulators in pyrrolidone solvents
AU739097B2 (en) * 1997-04-21 2001-10-04 Weyerhaeuser Company Method for inducing and determining maturity in conifer somatic embryos
US6143563A (en) * 1997-05-20 2000-11-07 Pioneer Hi-Bred International, Inc. Cryopreservation of embryogenic callus
WO1998057536A2 (en) * 1997-06-13 1998-12-23 University Of Saskatchewan Media and methods for culturing plant embryos
US7119262B1 (en) * 1997-07-31 2006-10-10 Sanford Scientific, Inc. Production of transgenic poinsettia
US6134830A (en) * 1997-11-20 2000-10-24 Weyerhaeuser Company Method for storing and improving the survival rate of conifer somatic embryo germinants
US6150167A (en) * 1998-02-19 2000-11-21 Weyerhaeuser Company Method of determining conifer embryo maturity using sugar alcohol content
AU758439B2 (en) * 1998-03-17 2003-03-20 Cellfor Inc. Maturation of somatic embryos
MD20000059A (en) 1998-05-15 2000-08-31 University Of Florida Regeneration system for grape and use thereof
CA2333184C (en) * 1998-06-01 2013-11-26 Weyerhaeuser Company Methods for classification of somatic embryos
US20040072143A1 (en) * 1998-06-01 2004-04-15 Weyerhaeuser Company Methods for classification of somatic embryos
CA2240135A1 (en) * 1998-06-05 1999-12-05 University Of Saskatchewan Technologies Inc. Increasing concentration of growth regulator during development of somatic embryos
WO1999065293A1 (en) 1998-06-12 1999-12-23 Silvagen Inc. A process for production and subsequent ex vitro sowing and propagation of pre-germinated plant somatic embryos
US6946295B2 (en) 1998-06-12 2005-09-20 Cellfor, Inc. Process for ex vitro sowing and germination of plant somatic embryos
AU776443B2 (en) * 1999-04-15 2004-09-09 Arborgen Inc. Enhancing germination of plant somatic embryos by priming
US6689609B1 (en) 1999-04-15 2004-02-10 Cellfor Inc. Enhancing germination of plant somatic embryos by priming
US6995015B1 (en) 1999-05-14 2006-02-07 University Of Florida Pathogen-resistant grape plants
DE60033536T2 (en) 1999-09-21 2007-11-15 Woody Plant Biotech Aps PROCESS FOR TIREING SOMATIC CONIFERE EMBRYOES
US6897065B1 (en) 1999-09-21 2005-05-24 Woody Plant Biotech Aps Method for maturation of conifer somatic embryos
US6893873B2 (en) 2002-01-25 2005-05-17 Georgia Tech Research Corporation Methods for improving conifer embryogenesis
US7598073B2 (en) 2002-04-09 2009-10-06 Weyerhaeuser Nr Company Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
US7381562B2 (en) 2002-05-30 2008-06-03 Weyerhaeuser Company Methods for producing cotyledonary pine embryos utilizing a gibberellin
CA2435337C (en) 2002-11-14 2010-03-23 Weyerhaeuser Company Methods for producing conifer somatic embryos
US7521237B2 (en) * 2003-06-23 2009-04-21 Weyerhaeuser Nr Company Methods for promoting maturation of conifer somatic embryos
US7530197B2 (en) 2003-06-30 2009-05-12 Weyerhaeuser Co. Automated system and method for harvesting and multi-stage screening of plant embryos
US7881502B2 (en) 2003-06-30 2011-02-01 Weyerhaeuser Nr Company Method and system for three-dimensionally imaging an apical dome of a plant embryo
US20040266002A1 (en) * 2003-06-30 2004-12-30 Weyerhaeuser Company Use of abscisic acid in somatic embryogenesis of pine trees
US7732205B2 (en) * 2003-07-30 2010-06-08 Weyerhaeuser Nr Company Development and stratification of pine somatic embryos using a liquid system
US7228658B2 (en) * 2003-08-27 2007-06-12 Weyerhaeuser Company Method of attaching an end seal to manufactured seeds
US8691575B2 (en) * 2003-09-30 2014-04-08 Weyerhaeuser Nr Company General method of classifying plant embryos using a generalized Lorenz-Bayes classifier
CA2484533C (en) * 2003-11-25 2008-12-02 Weyerhaeuser Company Systems and method of embryo delivery for manufactured seeds
US7555865B2 (en) * 2003-11-25 2009-07-07 Weyerhaeuser Nr Company Method and system of manufacturing artificial seed coats
US20050108929A1 (en) * 2003-11-25 2005-05-26 Edwin Hirahara Method and system for creating manufactured seeds
CA2486289C (en) 2003-11-25 2008-01-08 Weyerhaeuser Company Combination end seal and restraint
US7520089B2 (en) * 2003-11-25 2009-04-21 Weyerhaeuser Nr Company Method to improve manufactured seed germination
US20050108935A1 (en) * 2003-11-25 2005-05-26 Edwin Hirahara Method and system of manufacturing artificial seed coats
CA2486311C (en) 2003-11-26 2008-08-12 Weyerhaeuser Company Vacuum pick-up device with mechanically assisted release
US20050246802A1 (en) * 2003-12-02 2005-11-03 Attree Stephen M Bulk sorting of conifer somatic embryos
US7795029B2 (en) * 2003-12-04 2010-09-14 Cellfor Inc. Method of ex vitro sowing, germination, growth and conversion of plant somatic embryos or germinants
US7356965B2 (en) * 2003-12-11 2008-04-15 Weyerhaeuser Co. Multi-embryo manufactured seed
US7591287B2 (en) * 2003-12-18 2009-09-22 Weyerhaeuser Nr Company System and method for filling a seedcoat with a liquid to a selected level
CA2495650C (en) * 2004-02-25 2011-05-24 Weyerhaeuser Company Continuous culture of conifer embryogenic tissue
CA2496880C (en) 2004-03-02 2011-01-18 Weyerhaeuser Company Methods for developing conifer somatic embryos
US7568309B2 (en) * 2004-06-30 2009-08-04 Weyerhaeuser Nr Company Method and system for producing manufactured seeds
CA2518166C (en) * 2004-09-27 2012-02-21 Weyerhaeuser Company Manufactured seed having a live end seal
US20060064930A1 (en) * 2004-09-27 2006-03-30 Carlson William C Manufactured seed having a live end seal coating
US7547488B2 (en) * 2004-12-15 2009-06-16 Weyerhaeuser Nr Company Oriented strand board panel having improved strand alignment and a method for making the same
WO2006094400A1 (en) * 2005-03-10 2006-09-14 Cellfor Inc. Aerated liquid priming of conifer somatic germinants
DK1893731T3 (en) * 2005-06-23 2012-07-16 Dsm Ip Assets Bv Method for obtaining lipid from cells
US7654037B2 (en) * 2005-06-30 2010-02-02 Weyerhaeuser Nr Company Method to improve plant somatic embryo germination from manufactured seed
EP1931766A4 (en) * 2005-10-06 2009-09-02 Cellfor Inc Method of culturing conifer somatic embryos using s(+)-abscisic acid
US20070087438A1 (en) * 2005-10-17 2007-04-19 Grob James A Methods for conditioning plant somatic embryos
US20070099293A1 (en) * 2005-10-27 2007-05-03 Weyerhaeuser Co. Use of porous membrane to support developing conifer somatic embryos
CA2568476C (en) * 2005-12-22 2015-03-17 Weyerhaeuser Company Methods for storing conifer somatic embryo germinants
US8744775B2 (en) 2007-12-28 2014-06-03 Weyerhaeuser Nr Company Methods for classification of somatic embryos comprising hyperspectral line imaging
US8613158B2 (en) * 2008-04-18 2013-12-24 Ball Horticultural Company Method for grouping a plurality of growth-induced seeds for commercial use or sale based on testing of each individual seed
CA2647900C (en) * 2008-12-23 2017-05-16 National Research Council Of Canada Inhibitors of a 9-cis-epoxycarotenoid dioxygenase
WO2012087903A2 (en) 2010-12-20 2012-06-28 E. I. Du Pont De Nemours And Company Drought tolerant plants and related constructs and methods involving genes encoding mate-efflux polypeptides
EP2672802A1 (en) * 2011-02-11 2013-12-18 Coating Supply, Inc. Promotion of plant growth using collagen-based gelatin
WO2013063555A1 (en) 2011-10-28 2013-05-02 E. I. Du Pont De Nemours And Company Methods for the identification of genes involved in abiotic stress tolerance in plants
US9078427B1 (en) 2014-08-29 2015-07-14 Pioneer Hi Bred International Inc Method of storing plant embryos
BR112017003738A2 (en) 2014-08-29 2017-12-26 Pioneer Hi Bred Int methods of storing genomic DNA, storing molecular marker test materials, treating one or more plant embryos, selecting one or more plant embryos, and transforming plant tissue?

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005575A1 (en) * 1987-12-18 1989-06-29 University Of Florida Quiescent plant somatic embryos and method for their production
WO1991001629A1 (en) * 1989-08-01 1991-02-21 British Columbia Research Corporation A process for the production, drying and germination of conifer somatic embryos

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3920436A (en) * 1972-08-07 1975-11-18 Minnesota Mining & Mfg Artificial protective environment for plants
US4352883A (en) 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4217730A (en) 1979-05-07 1980-08-19 Weyerhaeuser Company Embryogenesis of gymnosperm forest trees
US4241537A (en) 1979-05-10 1980-12-30 W. R. Grace & Co. Plant growth media utilizing polyurethane hydrogel
US4249343A (en) * 1979-07-25 1981-02-10 Eastman Kodak Company Seed coatings
US4245432A (en) * 1979-07-25 1981-01-20 Eastman Kodak Company Seed coatings
US4251952A (en) * 1979-08-06 1981-02-24 Sandoz Ltd. Plant seed coating
US4353184A (en) 1981-05-18 1982-10-12 Weyerhaeuser Company Method for asexual reproduction of coniferous trees
SE442939B (en) 1981-10-09 1986-02-10 Milan Simak FRONT SORT SET
US4425178A (en) 1981-11-16 1984-01-10 Weyerhaeuser Company Resin hardener and method for its use
US4534781A (en) 1982-07-27 1985-08-13 Campbell Soup Company Mushroom supplement containing protein and a time delay coating
US4562663A (en) 1982-10-12 1986-01-07 Plant Genetics, Inc. Analogs of botanic seed
US4714679A (en) * 1982-12-03 1987-12-22 The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations In vitro propagation of grape
US4715143A (en) 1984-01-13 1987-12-29 Plant Genetics, Inc. Artificial seed coat for botanic seed analogs
JPS6140708A (en) * 1984-07-31 1986-02-27 ライオン株式会社 Artificial seed
US4615141A (en) * 1984-08-14 1986-10-07 Purdue Research Foundation Process for encapsulating asexual plant embryos
JPS63501263A (en) 1985-10-22 1988-05-19 プラント・ジェネティクス・インク Methods and media for improving somatic embryo formation
US4777762A (en) * 1986-01-07 1988-10-18 Plant Genetics, Inc. Desiccated analogs of botanic seed
JPS63133904A (en) * 1986-11-21 1988-06-06 ライオン株式会社 Artificial seed
EP0293598A3 (en) 1987-04-29 1991-03-20 DNA PLANT TECHNOLOGY CORPORATION (under the laws of the state of Delaware) Somatic embryogenesis and plant regeneration of cacao
GB8717099D0 (en) * 1987-07-20 1987-08-26 Univ Guelph Induce desiccation tolerance in somatic embryos
US5238835A (en) 1987-07-20 1993-08-24 University Of Guelph Process to induce desiccation tolerance in somatic embryos
JPH01218520A (en) * 1988-02-26 1989-08-31 Teijin Ltd Production of plant neoformation
JPH02504589A (en) 1988-06-14 1990-12-27 プラント・ジェネティックス・インコーポレーテッド How to mature somatic embryos for planting in a natural environment
JPH0231624A (en) * 1988-07-21 1990-02-01 Noboru Kaimori Production of artificial seed
WO1990001058A1 (en) 1988-07-28 1990-02-08 Plant Genetics, Inc. Method for improved somatic embryogenesis using synthetic auxin analogs
JPH0246240A (en) * 1988-08-08 1990-02-15 Nitsusen Kagaku Kogyo Kk Artificial seed
US5236841A (en) 1989-03-09 1993-08-17 Gupta Pramod K Method for reproducing conifers by somatic embryogenesis using stepwise hormone adjustment
US5034326A (en) * 1989-10-23 1991-07-23 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis using adsorbent materials in the development stage media
US5294549A (en) 1989-03-09 1994-03-15 Weyerhaeuser Company Method for reproducing conifers by somatic embryogenesis using mixed growth hormones for embryo culture
US4957866A (en) * 1989-03-09 1990-09-18 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis
US5036007A (en) * 1989-03-09 1991-07-30 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation
US5041382A (en) * 1989-03-09 1991-08-20 Weyerhaeuser Company High concentration enrichment of conifer embryonal cells
US5563061A (en) 1989-03-09 1996-10-08 Weyerhaeuser Company Method for reproducing conifers by somatic embryogenesis using a maltose enriched maintenance medium
US5482857A (en) 1989-03-09 1996-01-09 Weyerhaeuser Company Method for reproducing douglas-fir by somatic embryogenesis
FR2647465B1 (en) * 1989-05-23 1993-11-05 Nestle Sa Produits PROCESS FOR THE CONSERVATION OF PLANT EMBRYOS
FI88119C (en) 1989-07-06 1993-04-13 Valtion Teknillinen APPARATUS AND APPARATUS FOER PLOCKNING AV SMAO PARTIKLAR UR VAETSKA
FR2649860A1 (en) * 1989-07-18 1991-01-25 Nestle Sa PROCESS FOR THE CONSERVATION OF PLANT EMBRYOS
US5119588A (en) 1989-10-03 1992-06-09 Weyerhaeuser Company Method and apparatus for culturing autotrophic plants from heterotrophic plant material
US5187092A (en) 1990-03-22 1993-02-16 Institute Of Paper Science And Technology, Inc. Somatic embryogenesis in gymnosperms
ATE164080T1 (en) 1990-05-10 1998-04-15 Bechgaard Int Res PHARMACEUTICAL PREPARATION CONTAINING N-GLYCOFUROLES AND N-ETHYLENE GLYCOLS
CA2017417A1 (en) 1990-05-24 1991-11-24 Albert A. Marsolais Gametic and somatic embryogenesis using phenylacetic acid
US5288634A (en) * 1990-08-03 1994-02-22 Cornell Research Foundation, Inc. Method of increasing the percentage of viable dried spores of a fungus
US5236469A (en) 1990-10-26 1993-08-17 Weyerhaeuser Company Oxygenated analogs of botanic seed
US5427593A (en) 1990-10-26 1995-06-27 Weyerhaeuser Company Analogs of botanic seed
CA2075135A1 (en) 1991-08-02 1993-02-03 David E. Ellis Particle-mediated transformation of gymnosperms
WO1993019585A1 (en) 1992-04-01 1993-10-14 Union Camp Corporation Induction of somatic embryogenesis in taxus, and the production of taxane-ring containing alkaloids therefrom
US5284765A (en) * 1992-04-08 1994-02-08 Weyerhaeuser Company Method of directionally orienting plant embryos

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005575A1 (en) * 1987-12-18 1989-06-29 University Of Florida Quiescent plant somatic embryos and method for their production
WO1991001629A1 (en) * 1989-08-01 1991-02-21 British Columbia Research Corporation A process for the production, drying and germination of conifer somatic embryos

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Dialog Information Services, file 5, BIOSIS, Dialog accession no. 8125073, Biosis accession no. 91046073, Anandarajah K et al: "Manipulating the desiccation tolerance and vigor of dry somatic embryos of medicago-sativa L. with sucrose heat shock and abscisic acid", Plant Cell Rep 9 (8), 1990, 451-455 *
Dialog Information Services, file 5, BIOSIS, Dialog accession no.9084765, Biosis accession no. 93069765, Attree S M et al: "Enhanced maturation and desication tolerance of white spruce picea- glauca moench voss somatic embryos effects of a nonplasmolysint water stress and abscisic acid", Ann Bot (LOND) 68 (6), 1991, 519-526 *
Dialog Information Services, file 50, CAB, Dialog accession no. 1163544, McKersie B.D. et al: "Drying somatic embryos for use as articicial seeds. Proceedings of the Plant Growth Regulator Society of America 17th Annual Meeting edited by Hodgson, R.H.", Ithaca, New York, USA; Plant Growth RegulatorSociety of America 1990, 199-207 (7 ref.) *

Also Published As

Publication number Publication date
FI942909A (en) 1994-08-17
US5985667A (en) 1999-11-16
AU3154193A (en) 1993-07-19
WO1993011660A2 (en) 1993-06-24
FI942909A0 (en) 1994-06-17
US5464769A (en) 1995-11-07
ATE202261T1 (en) 2001-07-15
DE69231890D1 (en) 2001-07-26
EP0618766A1 (en) 1994-10-12
CA2125410A1 (en) 1993-06-24
NO942324D0 (en) 1994-06-17
NZ246137A (en) 1996-04-26
AU679435B2 (en) 1997-07-03
NO942324L (en) 1994-08-17
CA2125410C (en) 2000-03-28
DE69231890T2 (en) 2002-03-28
EP0618766B1 (en) 2001-06-20
US6372496B1 (en) 2002-04-16

Similar Documents

Publication Publication Date Title
WO1993011660A3 (en) Maturation, desiccation and encapsulation of gymnosperm somatic embryos
AU6897887A (en) Desiccated analogs of botanic seed
CA2086681A1 (en) Growth promoting agent derived from milk
CA2205188A1 (en) An improved method for regeneration of coniferous plants by somatic embryogenesis
EP0934691A3 (en) Improvements in or relating to embryogenic compounds
Gingas Asexual embryogenesis and plant regeneration from male catkins of Quercus
AU8484891A (en) Nutritional composition and process for preparation
Zayova et al. Effect of different auxins on in vitro rooting of Paulownia elongata propagated plants
DE59002676D1 (en) Process for removing pesticides from ginseng root.
ES8503231A1 (en) Preparation of a pharmaceutical camomile product
IL74568A0 (en) Thiocyanatopyrimidine derivative,its production process,and agricultural and horticultural fungicide compositions containing same
Griffin et al. Propagation of Thuja x ‘Green Giant’by stem cuttings: Effects of growth stage, type of cutting, and IBA treatment
CN216820998U (en) Fixing frame for peach tree transplanting
AU3842589A (en) Method for maturing somatic embryos for planting in natural environments
MD362G2 (en) Method of fruit trees crown cuttage
Salgado-Garciglia et al. Acmella oppositifolia micropropagation by single-node culture
ES2180385A1 (en) Production of cork haploid plants and embryos consists of gametic embryogenesis, germination, and accelerated overall growth
SU1340672A1 (en) Method of selecting early-ripening species of cucumber
Dhillon Growth of Black Currant Stem Cuttings Rooted Under Different Photoperiods
BR0113466A (en) Method of obtaining transgenic plant and transgenic plant
Jordán Zimmerman Morphogenic Responses and in Vitro Regeneration of Canelo (Drimys Winteri JR Et Forster), a Forest Species Used in Chilean Traditional Medicine
BG51472A1 (en) Izgrev variety peziwinkle (priwinkle)
HUP9800164A2 (en) Lympil variety of single cross hibryd sunflower (helianthus annuus l.)
RU93017326A (en) METHOD FOR FORMING CRONES OF FRUIT TREES
RU94019801A (en) METHOD OF REFINING STEVIA STEVIA REBAUDIANA BERTONI L. PLANTS OBTAINED IN CULTURE IN VITRO

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AT AU BB BG BR CA CH CS DE DK ES FI GB HU JP KP KR LK LU MG MN MW NL NO NZ PL RO RU SD SE US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AT AU BB BG BR CA CH CS DE DK ES FI GB HU JP KP KR LK LU MG MN MW NL NO NZ PL RO RU SD SE US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2125410

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 246137

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 942909

Country of ref document: FI

WWE Wipo information: entry into national phase

Ref document number: 1993900142

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 08244725

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1993900142

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 1997 985788

Country of ref document: US

Date of ref document: 19971205

Kind code of ref document: A

WWG Wipo information: grant in national office

Ref document number: 1993900142

Country of ref document: EP