WO1994028153A2 - Low-molecular dna from sturgeon milt, a method of obtaining the dna and a pharmaceutical preparation based on it - Google Patents

Abstract

The present invention relates to low-molecular DNA obtained from sturgeon milt and a pharmaceutical preparation based on it. Also proposed is a method of obtaining the said low-molecular DNA from sturgeon milt. Disclosed is the use of the new preparation based on the low-molecular DNA as a haemocorrector and inhibitor of viral activity, in particular HIV infections and mucous membrane diseases of various etiologies. Also disclosed is the use of the low-molecular DNA as a universal cosmetic additive.

Description

 ΗΗΚΟΚΟΟΟΕΚΕΚΗΚΗΚΗΚΗΚΗΚΗΚΜΟΜΟΜΟΜΟΟΚΟΚΟΚΟΟΟΟΟΟΟ, SPΟSΟB ΒIDΕΕΕΕΗΚΗΚΗΚΗΚΗΚΑΡΙ and δΑΡΙ / ΑЦΕΒΤΙ-ΙЧΕСΙШЫΡΕ ПΡΕПΑΡΑΤ ΗΑ ΟСΗΟΒΞ ДШ {ΚЗ ΙЛΟЛСΚ ΟСΕΤΡΟΒЫΧ ЫБ.

The invention is no longer available in the field of biology, medicine and medicine, and is subject to a small number of small children. new product

Yu on its basis and the method of obtaining the indicated DΗΚ. The product, according to the invention, possesses a wide biological activeness and may be used as a result of the ..., it is inactive. viral activity, in particular. Other infections, mucosal infections, etc.

15 terms. The product may also be referred to as a universal cosmetic additive in lotions, creams, dental elixirs and T..π.

It also has a cytostatic therapy, which is the main treatment for the treatment of arthritis, often causing

20 oppression of hematopoiesis, even in therapeutic doses (I). In this case, toxic myelo-depression is registered in patients with acute leukemia, leukemia, and further anemia (2). The treatment, which takes into account both chemo- and chemo-radiation therapy, limits the possibility of treatment, increases

25 list of ^' real infections and lethal outcomes (1,2). Сенρ- Senal of means, used in the case of depression, is quite different (3.4). Isποlzuyuτ πeρesadκu κοsτ- nοgο mοzga introduction leyκοmassy, κοlοniesτimuliρuyuschiχ φaκ- τοροv and τaκzhe τaκie πρeπaρaτy, κaκ sπlo ^'ning, leyκοgen,

30 pen ^ okeil, eimozan and vitamins are different groups (5-7).

However, the loss of a large brain is associated with an additional automatic intervention, and taking a leukemia mass can cause an immune infection. USE OF PHARMACEUTICAL AND MEDICINES OF PREPARATORY DEFICIENCY EFFICIENT AND WORKS

^• 35 yen chemotherapies.

In the process of searching for new biological active substances - 2 -

τοv bylο Εyyazleκο leyκοsτκmuliρutaschee deκsτzκe nuκleinοvyχ κislοτ (b-ΙΟ) .ι ^ ν ^ϊ ο προdemοnsτρiροvanο leyκοsτimuliρu- yuschee deysτvie πρeπaρaτοv on οsnοve vysοκοmοleκulyaρnοy DΗΚ, ποluchennοy of τι _,:. whiskers and selezenκi κρys (8.10). However, such

5 Drugs have a number of disadvantages: slow development of leukocytosis treatment and lack of stimulation of the process of booting.

In the case of a hemodialysis and leukopenia, the drug is used in the form of an injectable drug substance

Juvenile Acid Acid, obtained by hydrolysis from other countries (9). In addition to the painful injection, the drug has the same disadvantages: it only stimulates leukemia, is ineffective, and in the case of an indispensable drug

15 The efficacy of a drug containing a high molecular weight isolated from small herring in combination with daunorubicemia (II) is effective.

The salt of the low molecular weight derived from

20 lοκ οseτροvyχ ρyb not πρimenyavshayasya dο nasτοyaschegο izοbρeτe- Niya, οbladaeτ neοzhidashymιϊbiοlοgichesκimi aκτivnοsτyami: eφ- φeκτivnym sτimuliροvaniem leyκοποeza in τοm including κοsτnο- mοzgοvοgο κροveτvορeniya, sτimuliροvaniem healing πορa- zheny slizisτοy ρazlκchnοy lοκalizatsii and eτiοlοgii, inhibition

25 By cultivating ^' viral and microbial activity ^' , in particular, HIV infection.

For the present invention, with the suppression of HIV infection, nucleotide analogs, in particular, ΑЗΤ (Z-azi-3-desoxythymidine) were used (12,13). However, this is an ef-

30 φeκτiven only πρi ρanniχ eτadiyaχ ρazviτiya zabοlevaniya and da in the same τeρaπevτichesκiχ dοzaχ οbladaeτ vysοκοy τοκsichnοsτyο, πρivοdyaschey in κlinichesκοy πρaκτiκe κ οslοzhneniyam (gοlοvnaya bοl, anemia, naρusheniya sο sτοροny zheludοchnο-κishechnοgο τρaκτa) chτο οgρanichivaeτ egο isποlzοvanie (14,15). Κzve-

35 times the use and treatment of HIH infection for different drugs (16), the same effectiveness is small. Preparation _; - 3 -

According to the invention, has a high efficiency in the inhibition of inhibition of HIV infection in combination with low toxicity.

Efficiency has been demodel on models

5 lively and experimental gastric ulcers (17).

Pρeπaρaτ on οsnοve nizκοmοleκulyaρnοy DΗΚ of mοlοκ οse- τροvyχ ρyb προdemοnsτρiροzal neοzhidanκο vysοκuyu eφφeκτivκο- sτ πο οτnοsheniyu κ πορazheniyam slizisτοy οbοlοchκi ρτa and glοτκi, ρazlichnοy eτiοlοgii in τοm including κ τem, κοτορye

Yu is a consequence of the use of antibiotics, pharmaceuticals, heavy metals, and cassettes.

The method of radiation from the small fish fish in the U.S. СССΡ »Ι4Ι0494, 1986, 007421 (04). The method is concluded in the homogenization of milk in a standard citron-buffer

15 rе (SSC), separating the cell nuclei from the centrifugation, the homogenization and deactivation of the dedexylated Α / a and the temperature of 55 ° C. The chilled mixture is intensively mixed with kieselguhr (15.5%) and filtered. The target is planted with a ratio of 1: 1. Βysοκοπο-

20 dimensional DSPs and distributes sound to the magnet, filters, receiving 0.6 Ds in S / SSC.

The method of receiving D from the grocery store is known through the homogenization of milk, washing and discharging of food,

25 the privatization of the complex of D-white pructosubstilinum with the Sinusis spp.

Also known is the method of receiving D from the fish of the net, which implies the homogenization of the milk in the standard citrus-saline (0.87 net)

30 Russia), centrifugation, deprotection in the presence of de-decylsulfate for 1.5 hours at 60 ° С.

Subsequently, the suspensions add Λ / a ци and cit Λ / a to the final concentrations of 14.6 and §4, respectively, and warm up for 1.5 hours at a rate of 60 C. For separation

35 After cooling, open the casserole with kieselgum, on the other hand, (Dim. Pharmaceutical Journal, 1982, _ * 7, str. _ 4 -

835-838).

However, all the listed methods do not allow you to receive sodium salt without medical treatment. There are polisarids, even in small quantities in the drug

5 Unsuitable for use in medicine is undesirable because of its effects. The claimed method does not merely eliminate the indicated material deficiency, i.e. Allows you to receive the katruviy salt of DU without the use of polysaccharides, but also allows you to receive

The southern product is in the form of an appliance, which is very convenient for using the appliance, as well as for the preparation of different functions:.: Ace-process.

I. Method for the allocation of the invention.

15 Declared sποsοb allocation of DΗΚ mοlοκ οseτροvyχ ρyb, πρedusmaτρivayuschy gοmοgenizatsiyu zamοροzhennyχ mοlοκ in tsiτ- ρaτnοsοlevοm ρasτvορe, deπροτeinizatsiyu DΗΚ, denaτuρatsiyu belκa, degρadatsiyu DΗΚ, οτdelenie its οτ sπetsiφichesκiχ πρi- impurities and recovering tselevοgο προduκτa, οτlichayuschiysya τem, chτο

20 Before degradation, there are stages of dialysis and / or ultrafiltration.

Mostly, small-sized fish are genuinely standardized in a non-salable household, which has a good health of 0.8%.

25 0.54% .- The generator is heated at a temperature of 55-60 ° C in the absence of a de-sulphate (end-concentration 96) for 1.5 hours. Κ The mixture is then added to the product / WASD to an end concentration of 14.6% and a heating time of 1.5 hours at 55-60 ° C. The resulting mixture is processed with diatomaceous earth and

30 filters. The resultant device may be deactivated through an electric dialysis and / or ultrafiltration unit. Pοlu- chenny ρasτvορ οbρabaτyvayuτ ulτρazvuκοm, _{φilτρuyuτ,.} If necessary, concentrate, precipitate, impart alcohol, and wash 96% alcohol, dry it in an oven and dry.

35 UΡ ^{τ e} 40 ° ^C ποlucheniem suχοgο ποροshκa DΗΚ.

The DSh salt obtained as a result of the written process, - 5 -

has the following properties.

This white powder, commercially less than 80% (mass) of salt on the spot, low tolerance, not more than 1.5% protein, is more than 1.5% protein, over 17%

5 ^gi _*

Salt salt has a molecular weight of not more than 12

Η Д and a hyperactive effect not less than 37.

The product received in accordance with the declared declared method of use, is pleased to use the product, which is suitable for use in the media. * ^"

Ζ. The answer is.

For various purposes, various forms of preparation may be used according to the invention.

Аче In the case of hematopoiesis, the product is used

15 discharges of sodium salt of low molecular weight in the form of injections. On the part of the distributor, any pharmaceutical agent may be used, including the distilled Η ^ Ο and phis. pasteur. Injection is available in quantities that are effective for the demonstration of haemostimulation

20 active actions. It is preferable that the product is supplied with an injection product of 1% to 2;

The declaration of ownership of the declared property is subject to

25 water conditions with a traditional weight-lifting method (17). For this reason, a large quantity of food is added to the product, and a consumer is needed for receiving the necessary quantity of food. Further, the filters are filtered, sterilized, and the bottles are expanded. Аче On a pre-assembled basis, the manufacturer

30 take advantage of distilled water or physiological growth. The resulting product is free from any harmful fluid, does not contain mechanical or other impurities, is stable. Please try to store for a long time.

In case of infections of the mucous membrane and pharynx of various eti

35 lοgii isποlzueτsya ρasτvορ, sοdeρzhaschy DΗΚ in κοlichesτve, eφφeκτivnοm for healing of κazannyχ πορazheκy ^g. Previous

X- New name of Polidan dνθ 94/28 5

- 6 -

The solid capacitance of the product in the range is 0.2 to 2. Аче As a part of the manufacturer, in addition to physical equipment ... and a fully-equipped factory, you can use any type of pharmaceutical equipment. 5 For the suppression of HI-technology, they are used in combination with all-metal metals (zinc, nickel, flint, iron) in the ratio of 1: 1 to 1: 1000.

The receipt of metal systems is carried out by mowing the components and the separate relations and the temperature.

For the invention, as a universal universal product, the product is used in a variety of ways.

Κρem in κachesτve οsnοvy sοdeρzhπτ mοnοsτeaρaτ glitseρina 15 mοnοglitseρidy disτιυ_1_ροvannye or iχ adeκvaτnye zameniτeli, vοsκa emulsiοnnye, sτeaρin κοsmeτichesκy, τρieτanοlamin, glitseρin, maslο πaρφyumeρnοe, κοnseρvanτy, οτdushκu, vοdu.

The lotus for hair on a basic basis contains ethanol, sweethearts, gelatinous ingredients, glycerin, 1,2-20 π ρ ο душ душ и и и и and water.

The cultivation of the lotus is carried out by mixing the gelling components with water and glycerin and then the introduction of the other components.

In this case, we have a traditional way (18), by mixing 25 fat and one phase. Adding to the mass of the masses is subject to the conditions and conditions (temperature, environment, input).

The dental elixir on a basic basis contains ethyl alcohol, distilled glycerin, a perfume, and food products.

The preparation of the elixir is subject to a conventional method (18.19) by mixing the components. π е π з з τ з τ τ τ τ

aϊ.Τ-αl_nosti hall-_ιlenn

3.1. The study of the intrinsic activity of the claimed device was carried out in the test system:

- on an indicative bacterium in the Ames test αϊt ο ^η еϊϊα - 7 -

mixtures, с / аеδ e £. a2., ΡΛ / Α5, 1973, ν.70, ρ.228Ι

- In view of the large aberrations in the large brain cells before the operation of the trained machine (Gogs / S, Yαύι

; 1956, ν, 31, ρ.247) in the modification (Kalashchenko Α.Μ.

5 1977 and Zolotarova G.Η. with comp. 1978)

- By taking into account the dominant lethal indoctrination of indigenous rats in the infectious mouse cells (Lysn and I. c.

1978, ν, 39, ρ. 173-185).

3 open test system; concentrate of the drug was 10 la 0.1; 1.0; 10; 100 and 1000 mcg / ml.

The second series of experi ments of the products of the studied drug was prepared simply by the introduction. The drug was administered in a dose of 0.2 ml of solution / UASA in a dose of 5 mg / kg live weight (5 toxic doses of AD) in 100 mg / kg of weight 15 (100 U). The on-line group was entered in the same way by the same volume of the consumer. The same dosages were used in 3 series of experiments.

The analysis of the obtained results showed that in the research range of doses (from the substitute to 100 toxic 20 doses), the studied product is inactive.

3.2. Studying a drug in a ready-to-use medicine (1.5% solution) 5 entered the patient into a private Β the study used male mice Ρ * _| (СΒΑ χ С ^ ΒС) and СΒΑ, κρysys-males and females ϊ _ι αг and Ρ ^ (ΑΜ5У and ννс ^β έαг), domestic pigs,

30 dogs of the English Beagle and family of the chinchilla. Surveillance of the condition and live animals occurred within a month from the moment of the termination of the introduction of the drug. Biological and clinical analyzes of the blood, clinical analysis of the urine, and functional system of the heart-court system and so on were investigated.

Doses that are toxic, calculated on the basis of ο -

There, it’s Wilkoxon. After a month ..π.without the last station, ^• we have gone through an independent research. ο-

The death of the animals was not observed in any other case. Prior to the introduction of the drug, all of the indicated doses were available, so that the locally-annoying ones prevailed .; action. The awesome bad ones brought the drug. The weight gain did not differ from the other.

With the breakthrough of the majestic changes in the cities, it is not noted.

When the preparation was administered internally to mice of the same dose, doses of 600, 300 and 150 mg / kg were used (corresponded to 15 nd, volumes were 03, 0.15 and 0.07 ml per mouse).

The results are presented in Table I.

“BLITZ” I. Study of the toxicity of the drug in mice and the internal introduction

20

Dose (mg / kg)% of lost lively death (day)

600 0 300 0

25 150 0

It can be seen from the table that the dose of 600 mg / kg (amounting to 600 therapeutic doses for humans) does not result in death of animals. Weight gain in survivors

30 animals did not differ from the contact.

The study of the toxicity of complex compounds with the above-mentioned high-grade metals was available in doses of 5, 10, 20, 35 and 50, 75 mg / kg for non-linear mice, which were

35 injections. Observations of animals were carried out for 2 weeks, then calculated ____> 50 _' - 9 -

The results of the experiments are indicative that all of the following preparations were inexact with the interval from 5-75 v.: G / kg πρκ by any means of introduction.

Studies προ are given on κρыχ ы * _| (ΑΜСУ χ ϊνс ααг) male εχ with an average weight of 220-250 grams.

The results are presented in table ^9.

TABLE 2. Study of the toxicity of the preparation for a good introduction to the field

10

Dose group and regimen Total administration of live / whole (mg / kg)

I 30 mg / kg χ 5/24 g 0/15 150

15 2 6 mg / kg χ 5/24 g 0/15 30

3 ONLINE INTACT 0/15

Also, a study of the indicators of primary physical conditions (clinical and biochemical analysis) was carried out, we took an EEG, 20 divided the daily diuresis and examined the urinary tract. Every day, throughout the whole experiment, they shared the mass of living bodies.

For a practical study, we scored 5 live from each group by decoupling after the end of the 25th course on the 14th and 30th days after the course. Living animals were opened up, shared a lot of internal bodies and their weighty factors. Parts of the business community took a 10% share of the price of the raspberry, they cut the hematoxylin -zozinom, doubled the light of the world. 30 This study has shown that the product is easily transported by animals. Complications in the introduction and state of the animals were not observed. The introduction of the drug was not compatible with the pain syndrome.

Lively nourishing weight was reduced, weighted weight of 35 food items were dabbled in physiological matters. When studying the primary environment, there are significant changes - 10 -

κκй is not found out. Eritric patients, hemoglobic, easy to handle in the patient group, feel free to neglect them.

The study of the heart-vessel system according to the 5-ECG data showed that the drug did not affect the psychological function and the medical session.

When examining the function of a clinical trial, urine analysis revealed that the drug did not have a negligible effect on 10.

The results of the research carried out make it possible to conclude that the preparation is non-invasive and well-preserved even in high doses.

15 4. The use of the product as a hemostasis. 4.1. Experimental studies. Hemo-mediating properties of the drug were detected in studies using live animals using mice and dogs, under the conditions of an experimental treatment, caused by 20 cases.

For the first series of research experiments, the English Beagle dogs are weighing 11.5-15.6 kg. Plasma erythrocyte was caused by the administration of myelosan in a dose of 15 mg / kg single dose. Hydrocarbonate Salt D 25 was administered as an effective dose of 10 mg / kg and 25 mg / kg two and three days after the administration of myelosan. The total doses were, respectively, 20 mg / kg and 50 mg / kg. Because the consumer group received only 10 ml of physiologically acceptable product in the same case. On the 3rd, 7th, 14th, 21st, 28th, 30th, 42nd and 9th days of experience, we divided the number of patients in the practice of __: ^" crypotism.

The results of the experiments are presented in Table 3. As can be seen from the results, the introduction of 35 myelitis caused the discontinuation of the medications Β κοн- cl

ο

ШБШ.ЦΑ 3. The dynamics of the restoration of hemostasis in the dog when using the claimed medication on the basis of citations caused by a single administration of the drug in the doctor's office 15.

The total quantity of leukemia in the case of peripheral obstruction (LP) and the condition (Κ ШνΙ) in the form of α? The essence of the experience of ch1ρ§G48 ^{* "} 3 7 - ^" Ϊ4 2Ϊ - ^" - ^" 28 42 49

I _ ^{(as mg} /> κg2 lπκ ^• κκm Μ _w _ LPΚ κκm lπκ ^• shsh lπκ κκm lπκ κκm ^"LΙZH κκn

September 10.2+ 49.3+ 4.0+ 6.3+ 2.8+ 6.7+ 2.2+ 9.5+ 4.3+ 7.6+ 6.3+ 15.77.6+ 28.7 + Ι, έ 5.6 0 ^ 3 θ | 9. 0.4 0 ^ 85 0 ^ 65 1.2 _. 0 ^ 7 1.4 ϊ | s 3.4 1.555, &

ДΗΚ ΙΙ.8 + 51.2+ 6.4+ 7.1+ 3.8+ 10.9+ 3.0+ -12.83.813.4 + 6.8 + 25.4+ 9.8+ 32.3+ 20 mg / kg 0, Φ θ, 3 0 ^ 7 ϊ; З 0.55 Ι, ≤5 0.65 Ι, 3δ 0 ^ 470 ^ 906.98 ^ 8 ^ 4 2.4

DΗΚ

50 mg / kg 9.7+ 48.7+ 6.8+ II .3+ 4.3+ 12.2+ 4.6+ 13.6+ 5.913, ”8.9+ 27.3 10.944 ^ 5 + 0 ^ 8 7.1 1.3. 2.1 .. 0.67 Ι, δδ. 0.5. Ι, έδ. 0 ^ 95 1, 40 ^ 853.6 Ι, 5656.6

Note: myelosan was introduced into the stomach,

They entered on the 3rd and 7th days after myelosan,

Lipoproteins - peripheral lymphocytes, ΚΚΜ - obstructive pulmonary disease.

- 12 -

A large group of patients and karotsitsy at a livelihood remained low to 28 days of a trial and began to run, from a week to a day

5 Introducing salt to the Republic of Kazakhstan has significantly changed the safety of the wiring and the wiring of the mains. Well, in both groups, and the introduction of sodium salt in the total doses of 20 and 50 mg / kg,

SJ enjoyed a less profound leukemia.

The tendency for the restoration of the quantity of drugs in patients was noted already from 21 days of experience, and for the end of the treatment the quantity of drugs was observed for the patient. A similar bargain was observed in Russia as well.

15 laziness of the obstruction of the obesity.

In general, in this series of experiments, it was indicated that sodium salt was a total of 20 mg / kg, and it is generally more acceptable.

20 rhenia in case of suppression of hematopoiesis, caused by mesosanosis.

This series of experiments was carried out on mouse hybrids

, males weighing 18-20 grams. Cycling was caused by introducing a cycle inside the system.

25 n.a. single dose in doses of 200 and 275 mg / kg. Sodium salt was administered in a convenient, single dose of 150 and 30 mg / kg, and also on days 3, 5, 7 after the introduction of the cycle. In I, 3, 5, 7, 10, 12, 14, 17, 21, and 25, the days divided the total number of leukemia patients in use by the general public.

30 cameras Goryaeva. The results of the experiment are presented in Table 4.

As can be seen from the results presented in Table 4, the maximum cyclic effect of the introduction of the cycle is resolved in 3 days, the restoration of

35 cells are noted for 7–9 days. However, at 12-13 days, there is a second, less deep decrease in quantity - thirteen -

TABLE 4. The dynamics of the restoration of hemostsis in mice by using the claimed medication based on the citation of a single cycle of 200 mg (_).

Personally treated patients kp <? __ and (thousand for I mm3) experience

(mg / κg)

10 5 8 10 12 15 17

End 13.9+ 2.42+ 4.46+ 6.8+ 8.34+ 7.58+ 5.94+ 6.38+ (without 3.4 - 0.27 ^" 1.04 ^" 2.85 0, 77 ^" 1.65 ^" 0.88 ^" 2.31 ^"

DΗΚ)

ДΗΚ _Τ ο _τ _ 2.62+ 8.26+ 8.86+ 7.38+ 8.74+ 8.02+ 8.24+

15 30 ^ ± ο, 44 ^" 1.8 - 1.7 ^" 1.04 ^" 0 ^ 55 ^" 2.42 ^" 4 ^ 73 ^"

(one-time)

12.1+ 1.9+ 6.38+ ^> ± 0Ι82 ¹ 7.7+ 7.88+ 7.52+

_30 2.6 ^" 0.7 ^" 0 ^ 7 ^"ι ^ Ι, 8Τ ϊ | 37 ^" 1.37 ^" (one-time)

20

Κ 11.6+ 2.44+ 6.98+ 10.5+ 11, 64 + 8.94+ 7.96+ 8.86+

0 2, ST 0.55 ^" 1.55 Ι, 4g 2.36 ^" 2.36 ^" I 43 ^" 18 "

(on 3,5 and 7 days)

25 leukits. With the introduction of D at a dose of 150 mg / kg for 3.5 and 7 days, a maximizing stimulating effect is achieved. Therefore, after 2–3 days after the first injection, a large number of cells is returned to the prior level of myelosin administration, and 7–10 days after treatment

30 magnitude, increasing this level. It is important to note that, in the absence of other gemstones, they do not cause a malfunction in the case of hemostasis, ^{”he said} .

Лич The distinction is made by a well-known product - 14 -

Live use is subject to the rapid recovery of medication, and the treatment of patients is To obtain a better effect,

5 single daily injection π е π ΗΚ ΗΚ ΗΚ ΗΚ ΗΚ ΗΚ ΗΚ ΗΚ. Β That is, time, κεκ πρκ of use ж other, the effect is achieved only after ten days of treatment. Declared that they do not cause an immediate reduction in the number of cells after haemostimulation.

10

4.2. Malefic research.

Clinical studies of the claimed drug were carried out in a patient with oppression of hematopoiesis due to the use of chemo-radiation therapy. Research

15, a group of 30 sick people aged 6 to 14 years was diagnosed with a diagnosis of lymphoma, lymphoid leukemia, and others. As a result of treatment for patients with radiation and radiation, the patient under study developed leukemia (from 100 to 1500 in mm3 of disease).

20 The treatment was obtained using a 0.5–2.5-year-old low-molecular diet product from a small fish farm. Dosages and quantities of injections varied depending on the course of the underlying disease. More preferable was an interval of up to 7 mg / kg to

25 35 mg / κ ^' g, which ensures an increase in leukemia by 3-5 times as a result of 1-2 injections.

The results of the research are presented in Table 5. The study group also had a myelogram analysis, which was not used only for testing.

30 κοsτimuliρuyuschee dis ^'τvie, nο and yaρκο vyρazhennοe influence on nορmalizatsigo megaκaρiοtsiτaρnοgο κροveτvορeniya (τablitsa] ^5' 6) Τaκim οbρazοm, zayavlennοe sρedsτvο οbladaeτ not τοlκο leyκοsτimuliρuyuschim deysτviem, znachiτelnο πρevyshayuschim analogous lοgichnοe deysτvie πρeπaρaτοv izvesτnοgο uροvnya τeχniκi, nο

35 and unknown prior to the stimulation of free-space activity. - fifteen -

TABLE 5. Dynamics of conversion in an oncologic patient in the process of chemo-radiation therapy and application

There is an approximate 5 dynamics of leukemia of the main- type of treatment, before the introduction of the drug is administered

30 Ц0ΙШ, ЦППП 100- 450- προдолж, 2-3 inc

DAPP 1,500 5,000 food per day and / or full capacity of bicin

П0ΙШ, ЦΒПП, Д0ΙШ and beam τеρапия

Фаρмορ-tubicin and radiation therapy

Note: ЦОШΙ - cycle, onkvin, низprednizilon, ρροοбббббΒΒΒΒΒΒΒ - - cycle, φκбласласлас,, ,ии, πедедедвин ,винвинвинвинвинвинвинвинвинвинвинвинвинвин Двин Дазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазазаз

More than that, in the absence of known equipment, there is no unexpected phase of the reduction of the incidence of hemorrhage. An important advantage of the device is that a consensus of the invention and the application in the clinic is the possibility of a basic treatment, that is, there is no need to replace the course of chemotherapy, which is of decisive importance in the treatment of malignant diseases. - 16 -

ΡTHE PERFORMANCE 6. Myelogamma dynamics (in%) when used

ϊ ?. INDICATORS BEFORE THE INTRODUCTION After the introduction of the маορma ππ myelogma polidana polidana

I Neurological myelocytes - 14.0 7.0-12.2

All cells are gnarled-

10

fifteen

20

A comparative method of known drugs used in chemo-radiation myelo-depression, and the device is amended by the invention. 7

Glucose - / acid salt of nucleic acid),

25 The resulting hydrolysis of others is used in the treatment of hepatitis • as a hemostasis. The fact of stimulation of large groups and macrophages is the most frequently used hemostasis, which is the result of treatment.

30 The use of general instructions for the use of basic food products is subject to change.

35 - 17 -

TABLE 7. Comparative treatment of hemostatic drugs, used in patients with chemo-radiation myelopathy

A comparable product of the medication that stimulates the activity of the drug on the basis of a highly cultivated animal from the thymus and spleen is kept away from the house. - 18 -

PERSON 8. Comparative treatment of the stimulation of leukopoiesis (live)

Loss of speed

Received from 15-25 days there is no thymus and spleen krasys Yu 2 mg / kg

4-5 injections (κρыы)

Received from 8-21 days, a single-day baby of the osetra-stimulation of fish out of the body of the earth.

15 days for manufacture: 20-50 mg / kg two-fold, for mice: 30-150 mg single-, tri-

20 and five

These experimental and clinical studies have unambiguous evidence of a highly effective gemstim 25 of the declared performance of this application. non-toxic and non-magnetic, which makes it possible to use a wide range of drugs. in κliniκe in κachesτve gemοκορρeκτορa shiροκοgο sπeκτρa deysτviya- not τοlκο πρi tsiτοπenii in bοlnyχ ρaκοm, vyzvannοy χimiο-luchevοy ^{'τeρaπiey,} nο and ρadiatsiοn- 30' nοy medicine πρi duchevyχ zabοlevaniyaχ and τaκzhe πρi dρugiχ sοsτοyaniyaχ, svyazannyχ sο sdvigοm φορmuly κροvi.

5. The use of the drug as a medicine that inhibits the general HIV infection. 35, The present invention was not aware of the known positive acidity of nucleic acids. For the prevention of HIV infection, the invention is subject to the use of electronic products. - 19 -

DΗΚ of mοlοκ οseτρa of sοcheτaκκi with ποlivalenτnymi meτallami (tsinκ, niκel, κοbalτ, zhelezο) in sοοτnοsheniyaχ οτ 1: 1 dο 1: 1000, and administering πρeπaρaτa οsuschesτvlyayuτ ποdκοzhnο, vnuτρκ- bρyushκκnο, κκτρanazalnο or sπκnnοmοzgοvοy κanal in dependence simοsτi οτ Forms of disease, in therapeutically equivalent to ^x doses. The achievement of a patient with a different effect and the different methods of administering the drug is very important for a different case of AIDS AIDS - it’s very difficult to get sick. The high efficiency of the harmony of the invention in the suppression of HI-infection has been identified in the following experiments.

To suppress ZICH in the culture systems, the cells used D-complexes with zinc, calcium, nickel, and iron, and various concentrations of various components. For comparison, we used the company’s company "

. ''

The effective activity of the drugs was evaluated using methods that were recommended for this purpose ΒΟЗ (20.21). The cultures of the incisive cell lines CΕΜ - and ΜΤ-4 were used. The culture cells were cultured at a concentration of (0.03-0.05) χΙ0 ⁶ cells per I ml of medium ΡΡΜΙ 1640 with 10 feces of calves, 300 mg / ml of glutamines, 100 mg / ml of samples were obtained. The livelihood of the cage was removed by cutting the 0.4 blue color. On the other hand, the sources в /// U and Ηϊν - I- ΗΤЗ / ΙΙΙΒ were used as sources of viruses.

The suspension of the cages was placed in 24-hole panels, processed with various doses of the preparation and infected with HIV. The infectiousness of infection was 0.01 ΤС & rear for the car. After this culture, they incubated at 37 ° C in the atmosphere, which contained 5% of humidity and 98% humidity during 5-7 days of treatment of the patient.

To evaluate the formation of viral antigen, an immunoassay analysis was used. The results are presented in table 9. - 20 -

PERSONAL 9. EFFICIENT SUPPLY

10

fifteen

20

25

thirty

35

- 21 -

From the table below it is visible that when introducing into the cultural environment the complex of D-metal is noted, the prevention of HIV infection is noted. Moreover, the optimal ratio of metal is in the range 1: 1 - 1: 1000. With this virus-5, the static effect is combined with a low frequency.

Лич The difference in the declared method, when introducing into the cultivated environment of the drug, there is a high frequency of moderate waiver.

Subsequently, the toxicity of the preparation was agreed upon by experiment 10 on experimental live animals.

Gesta was administered in parallel to all 4 types of drug in comparison with azidothymidine in doses of 5, 0, 20, 35 and 50 mg / kg. Issledοvaniya τοκsichnοsτi cheτyρeχ τiποv πρeπaρa- τa and azidοτimidina προvοdilis on nelineynyχ belyχ myshaχ 15 vesοm 6-7 gρammοv with isποlzοvaniem πρeπερετοv in ρaznyχ dοzaχ in φορme ποdκοzhnyχ, and inτρanazalnyχ vnuτρibρyushinnyχ and inτρatseρebρalnyχ ineκtsy. The animals were kept under observation for 2 weeks, and then they calculated the Siegeg method. At the end of ^ - ^ 50 ^π Ρ, the ^{minimum amount is} ρ ^{ρ π π} ,,,,, causing 20 total deaths of 50 animals.

The results of the investigation of the toxicity of these practices are given in table 10.

TABLE 10. Partial and antique activity of the complex 25 D-metal and various introduction modes

FIRST & Research - Supplementary administration of Dose of the preparation, mg / kg of the second-generation denia syncytate 5 10 20 35 50 75 τiev in% ₃₀ - -.

I ДΗΚ and / tserubeba-. . . . flax 6/66/66/6 6/66/6 6/6 25

Ζ _»n οdκοzhnο 6/66/66/6 6/66/6 6/6 and 25 / abdοmina- _m. flax 6/66/66/6 6/66/6 6/6 25

35 and / nasal 6/66/66/6 6/66/6 6/6 25 - 22 -

Location τε of Table 10

2 ДΗΚ- and / ЦЕЕБЕБАРАЛО 6/66/66/66/66/66/6 15

5 And on 6/66/66/66/66/66/6 15 and / abdominal 6/66/66/66/66/66/6 15 and / each 6/66/66/66/66/66 / 6 15

3 ДΗΚ- and / ЦЕЕБЕБАРАЛО 6/66/66/66/66/66/6 31

10

So it is 6/66/66/66/66/66/6 32 and / abdomi-. . . . . . cash 6/66/66/66/66/66/6 33 and / nasal 6/66/66/66/66/66/6 31

4 ДΗΚ- and / Цереебρ-

15 flax 6/66/66/66/66/66/6 g available on 6/66/66/66/66/66/6 and / εbdo--. . . . . . n / a 6/66/66/66/66/66/6 and / nasal 6/66/66/66/66/6 b / w

5 ΑЗΤ and / tseρebρε-

20 (aziditlno 6/66/66/66/66/66/6 10 midin- ⁾ _useful b / 66/66/66/66/66/6 and / abdomi-. . . . . . cash 6/66/66/66/66/66/6 and / nasal 6/66/66/66/66/66/6 75

25 Placebo and / tserabeba-. . . . . . flax 6/66/66/66/66/66/6 100 is available 6/66/66/66/66/66/6 100 and / abdomi-. ,,, cash 6/66/66/66/66/66/6 100 and / nasal 6/66/66/66/66/66/6 100 30

NOTE: The numerator is the number of surviving mice; the denominator is the number of infected mice.

It can be seen from the table that all five types of factors, nevertheless 35 doses, appeared to be toxic to white mice, weighing 5-7 grams, in general, there was no harm ^> .

minimum star (observation period - 2 weeks). 3 at the same time, 50% of the dose is 50 mg / kg and intramuscular administration among mice is πρκ πρ-overexposed. The additional midin caused a 50% mouse miscarriage and an irrelevant administration in doses of 10 to 50 mg / kg live weight. Χaρaκτeρnο, chτο in issleduemυχ κοmπleκsοv, πρaκτiche- sκi πρκ lyubοm sποsοbe administration sοχρanyaeτsya προτκvοviρusnaya aκτivnοsτ in το same vρemya, κaκ in ΑZΤ οna better vyρazhena πρi vnuτρitseρebρalnοm, ποdκοzhnοm and vnuτρκbρyushinnοm introduction.

Analysis of the experimental data is a one-to-one testimony to the pronounced anti-harmonicity of the product in accordance with the introduction of a low

6. The study of the active activity of the complexes

The metal was also used in the case of viruses, germs and viruses with the use of the well-known methods (Heb. αϊ,, 1982, ν.18, ρ.Ι69; ΑЕеη & .Ρ., Α δ. X

1983, ν.44, ρ.263), πρ data are given in table II

ΤΑ BLITZ Α II. Investigation of the industrial activity of complexes in the metal

The type of researched used systems is the percentage of viruses that are connected to the average data)

Culture group of the embryos 21-24% of the family culture of the cells

25-32% Influenza Viruses Cultivate Cleotus-45-, - 24 -

From the table below, it is visible that the complex prevents access to the most efficient virus that is most pronounced in the treatment of the herpes virus.

5 7. Stimulation of the healing of mucosal lesions.

The efficacy of nucleic acids in the healing of diseases of the oral mucosa and pharynx was not known to the present invention. The product is agreed to by the company that possesses the ability to stimulate the healing of diseases of the mucous membrane of the mouth and pharyngeal of various etiology. Ηaibοlee aκτualnσy yav- lyaeτsya προblema zazhiΕleniya πορazheny slκzisτοy οbοlοchκi πο- lοsτi ρτa and glοτκi, κοτορye yavlyayuτsya chasτymi οslοzhneniyami πρi mesτnοm and πaρenτeρalnοm administered anτibiοτiκοv, πρeπa- ρετοv τyazhelyχ meτεllοβ, tsiτοsτετiκοv (22-24). 15 A study of a wide range of inventions (from 0.2 to 2%) showed a comprehensive study of all the studied data.

The results of clinical studies on the effect of 20 20 testicular salt on the dynamics of treatment of mucosal disease. Observations were made in 48 patients with basic diseases - lymphatic disease, acute sarcoma, simple leukemia, and lymphoma. 25 The treatment of the sick was done by chemotherapy, in particular, by the meta-technologist, by the manufacturer, otherwise, by the lower and others.

In the process of therapeutic treatment for 3-5 days in patients in the area of the mouth and throat, there were phenomena

30 gates, gingivitis.

When used: the mucous membrane of the body is overexposed, it is infinitely clean, contains a large number of nice elements that are covered with white paint, which are lightly white.

With liquid food, it is troublesome, whereas it is unlawful. At 35 sickly patients, a subfebrile temperature was noted.

The most painstaking experience was given to the public at home. 25 -

3 day

6-8 times, depending on the severity of the process, β for 5-6 days. At all painful courses, there was no disruption at all.

ΤΑΕSh'SCHΑ 12. Dynamics of clinical manifestations of leukar-indirect pharyngo-gingivitis-disorders and application: DΗΚ

Type of treatment- Effectiveness of treatment (per day)

10 life ^* mucous disappeared disappeared disappeared completely complete nosiness naming nasal nascent ugugra ugivanie pain in the case of hyperaemia of a waste of food

15 Costs for 2 2-3 4-5 2-3 6 day day day day day

Gingiva- on 2-3 2-3 5 day 3 day 7-8 day costs day day

Gingivitis on 2 3 day on 7 on 2 9 day day day day

20 paring- for 2-3 3-4 8 day 2-3 10 day cost day day day

From the presented table, it is visible that already on the 2nd day of treatment, pain was better in the patients, but 2-3 days later

25 there was food, more than 4 days and food.

Ηε 2-4 day purchase is more efficient. Hyperemia disappeared on days 4–8. However, the reduction of hyperemia here may be associated with the recovery of granulated processes leading to an acceleration of the epithelialization of an acute rash.

30 For all observed cases, a full process was observed on day 6-10. It should be noted that healing took place on the 6th day, gingiva stays on the 7-8th day, gingivitis on the 9th day. The longest healing was noted in the case of a clear localization of προ-

35 cess. Here, the process was only purchased on the 10th day. - 26 -

Αnεlκz κlinichesκiχ dεnnyχ οdnοzκεchnο svideτelsτvueτ ο τοm, chτο aππliκatsii ρasτvοροm ποlidana dayuτ vοzmοzhnοsτ eφφeκτivnο κuπiροvaτ leκaρsτvennye πορazheniya slizisτοy οbοlοchκi ποlοsτi ρτa and glοκτi (sτοmaτiτy, gingiviτy, gκn- givοsτοmaτiτy, φaρingο-sτοmaτiτy). An important advantage is that the treatment does not require a change in basic profitability and even a reduction in the cost.

TABLE 13. Comparative action of the drug has been performed with known drugs in the treatment of medication for the treatment of mucous disease

Effects of the Method of Effectiveness of Influence on the Condition of not being able to maintain the dynamics of the mucous membrane of the main basic condition

(with the treatment of ill-health of a cold drink)

6–10 days have been expired. Further epithelization - application of the cusp, in the absence of a process of function

Normal 7-14 days π-д---Я зв Я зв зв κ κ τ τ τ τ τ. local process, antiseptics sometimes ulcerative-gangrenous

Reset: 7-14 days Elimination Epithelisation-antigestion, minor, chemo-analgesic therapy

From the presented table, you can see that the use of the menu for the use of the menu has been shown to the advantage of beneficial effect on the dynamics of the outbreak. Trying to administer chemotherapy and traditional treatment caused aggravation of the process in the mucous membrane and the - 27 -

It is located in a peptic ulcer and sometimes peptic ulcer-gangreous forms.

The efficacy of the drug in the treatment of mucosal infections is to use it extensively in the medical field. 5

8. Aggregate deactivation.

The product is in accordance with a proprietary method with an efficient and commercially viable action. Ε In addition, the biological dietary supplement has not been used before. SE 8.1. Efficiency of the declared drug as a universal biological supplement in the process of studying medical therapies

15 skobema, containing in the quality of biological. The active supplement is the sodium salt of DΗΚ, according to known methods (25–26).

Upon exposure to krεε, activation of harmful processes was revealed in each case, in addition to the absence of annoying and 20 allergenic activities.

A study of the effect of the declared volume on biochemical indicators revealed that it also increases the content of total protein and protein in general. In experimental animals, a good occurrence of labeled 25th With thymidine was detected after a cutaneous barium. Also found were antioxidant, pho-protective and hydrant properties. Received at the same time provides a special emulsion of light-white or white color with a good reserve. It is absorbed well and does not leave film on the skin. 30 Results of experimental clinical trials are summarized in table. ° 14.

35 - 28 -

* "__

TABLE 14. The medical and therapeutic properties of the complex, which are contained in the biological active dietary supplement of Salt

Properties under study Result research results (source of information)

Occupancy 19 METHOD

Note 26 It is noteworthy to have a positive effect on the sea with changes in both the macro- and phylogical study of the skin and the interior

Contents of the Schmid Method- Increase in protein from 1.52 total leaves with 2.32 meals and protein, and increase in protein from 21.0 to 27.4 mg / year Dynamo S ' 1π Lipid increase with (27) 4,373.0 to 445 mg / g

The protective circuitry of the circuit packs does not have to be connected to the radio at the same time that the radiation is completely free of 280 nm ".

(28)

Antioxidant Unit е- Full Compressor Pressur ρ Reduced Oxidation of Lipids

(18,27,28)

Hydroelectric power plant; Expressed hydraulics have been identified with indirect systems (the Turkish Demand Water System); the method is (29)

These svideτelsτvuyuτ ο τοm, chτο introduction sοs- τav κοsmeτichesκοgο κρema biοlsh.ichesκi aκτivnοy dοbavκi - naτρievοy sοli DΗΚ ποzvοlilο ρasshiρiτ sπeκτρ biοlοgiche- sκοy aκτivnοsτi κρema, πρi οτsuτsτvii alleρgiziρuyuschegο deysτviya and dρugiχ ποbοchiyχ eφφeκτοv. - 29 -

8.2. The study of the medicinal and therapeutic properties of the forest for the hair, which contains the biological addition of sodium salt.

The study of the therapeutic and active activity of the forest is only 5 for the Volos, which is contained in the biological active supplement, which is carried out in the human body. Eκsπeρimenτy προvedeny on ποlοvοzρelyχ zhivοτnyχ on ^χ οdyaschiχsya κa sτandaρτnοm ρatsiοne vivaρiya. With the experience of using 20 males ^χ pigs of males-albsh-yusov with a mass of 10 bodies of 350-400 g and 5 units weighing 2.5-3.0 kg.

^• We studied the toxicity of Gatsu 27 (27). Evaluation of the destructive effect was achieved by the influence of the preparation on the mucous membrane of the eye of the public. The researched forest was also applied daily for 21 days for a period of 15 days with a size of 2x2 cm.

At the seaside pigs, we studied the effect of the forest on the linear growth of hair. For this purpose, a livestock was cut on a separate section and processed it for 21 days, 0.5 g of a lotus. As of 7, 14, 21 days, they pulled out a wave of 20 s, measured the length. We studied the structure of Volodya, compared the results with the control. 21 For 21 days we studied the toxicity of the drug. Observed the general condition, aggregate, weight gain of the body, the state of the body, arms, the presence of the body. They studied biologics, both from the skin and internal bodies, both physically and physically.

Clinical trials were carried out in patients aged 14-62 years with a working floor with symptoms of seborrhea and a diffuse loss of hair.

The lotus got in during 21 days, daily. Before the beginning of the course of treatment, you will receive good care for 24 hours before closing the call. Results of experimental clinical trials:

The forest is not toxic. She does not have a irritating and allergic effect. 35 At the same time, they noted the regeneration of epidermal cells, an increase in the quantity of LJ in the skin, an increase in - thirty -

- where the functional activity of the ΙΪΤΟΒΠΤΤΤΟΟΟΙΪΤΟΒ and mast cells is.

Established a positive impact on the structure of hair, the strengthening of the green bag, the weight of the hair,

5th of the barracks.

The company owns good public health services, is easy to relieve in the majority of the world, and does not possess any allergies: v.

The results of observations in ill patients showed

Even after a 21-day course, their hair is soft, spreading, and the quantity of frustration has decreased. The film under construction protects against atmospheric influences (protective effect). The loss of hair is reduced due to the antioxidant effect. Pοluchennye data demοnsτρiρuyuτ, chτο introduced into 5 denie sοsτav lοsοna for vοlοs biοlοgζchesκi aκτivnοy dοbavκi - naτρievοy sοli DΗΚ ποzvοlilο ποluchiτ eφφeκτiv- ny πρeπaρaτ for vοlοs, snshvaschy προyavlenie sebορei, πi- τayuschy vοlοsy, οbladayuschy φοτοzaschiτnym deysτviem.

2 8.3. The study of the therapeutic and therapeutic activity of the elixir for teeth, which is contained in the quality of the biological supplement sodium salt.

Clinical research indicates that the dental elixir has a beneficial effect. It accelerates the healing of normal mucous membranes. The biological tests revealed an antiseptic activity in the elixir. The clinical appraisal of the elixir revealed better • some ^“ good things” that cleans the action, as well as a positive effect on the patient. For example, sodium salt, 0 days, according to the invention, receives a dental elixir, is anti-inflammatory, anti-allergic, and anti-allergic.

Local allergy products that were used with all commercially available products using the general 5 methods (26) did not reveal any allergenic properties. - 31 -

Ηa οsnοvanii πρivedennyχ ρezulτaτοv mοzhnο sdelaτ vyvοd, chτο naτρievaya sοl DΗΚ yavlyaeτsya uiiveρsalnοy κοs- meτichesκοy dοbavκοy, πρimenenie κοτοροi daeτ vοzmοzhnοsτ ποvysiτ lechebnο-προφilaκτichesκuyu eφφeκτivnοsτ κοsmeτi-

⁵ garlic.

In general, according to the invention, it is a universal add-on and can be used in any kind of inaccurate medical treatment.

Skills and other personal effects do not have a negative impact on the normal structure of the skin and domestic amenities.

All this means that you will be able to take a wide range of roads.

^ 5 readings agreed upon as an additive in the consumer product.

The invention is illustrated by the following examples.

PSI I. 20 Bolnoy Κ. In the case of myelotherapy, caused by the administration of chemo-radiation therapy, the introduction of 1.5% of sodium salt in the amount of 5 ml was carried out.

If, before the introduction of the drug, the treatment amounted to 100-300 in mm3 of the circuit, then after the introduction, there is a noticeable 2 increase in the number of drugs. On the first day after injection - 700 in mm3 of the tank, on the second day - 1000, on the 3rd day - 4200, on the 5th day - 5100. The result was also a positive increase in the outcome of the group. / - (issue 57 /.). 30 The index of the eruption of erythritis before treatment with the claimed medication is cut to zero, after treatment 0.8% (rate 0.7-0.9).

Complications of the general disease, as well as irritation or soreness at the injection site, have not been identified.

35 - 32 -

ПΡИΪ.ΕΡ 2.

Patient Α., 13 years old, was admitted to the Department of Pediatric Economics on 07/13/92. The diagnosis of a simple lymphoblastic was diagnosed: leucosis. Pροveden κuρs imiοτeρaπii ^χ (tsiκlοφοsφan, vinκρis- 5 τin, ρubοmitsin, πρednizοlοn). Уль As a result, the growing leukemia was detected. 11/25/92 quantity of drugs - 2500, 11/27/92 - 400, 11/28/92 - 300, 11/29/92 - 100. On 11/30/92, 1.5% of the rp-ρ was delivered in a quantity of 5 ml. Β As a result, there was an increase in the quantity of le ^ κο-

10 quotes

Τaκ already 2.12.92 κοlichesτvο leyκοtsiτοz - 700 12/07/92 - 4200 10/12/92 - 5000. Οdnοvρemeκnο οτmechalas nορmalizatsiya mielοgρammy: uvelichilοs κοlichesτvο mielοtsiτοv, eρiτροκaρiοtsiτοv, 15 ποli- ^χ ροmaτοφilnyχ nορmοtsiτοv, chτο svideτelsτvueτ ο sτimulya- κοsτnοmοzgοvοgο κροveτvορeniya tion.

As a result of the hemopoiesis, the patient did not disrupt the course of chemotherapy. However, during the treatment of reducing the number of leukemia in the future was not noted. Introduction 20 of the product was not triggered by natural effects.

ПΡИΜΕΡ 3.

Κ Experiment used the cultured cell lines of СΕΜ55 and ΜΤ-4. Cultural cows cultivated rivals

25 cents 0.03x0.05x10 ° cells per I ml of medium ΡΡΜ7 1640 with 10 fetal calves, 300 mg / ml Ζ. glutamine, 100 μg / ml gentamycin and cultured in suspension. The ability to sell the cassette was prevented by cutting 0.4% of the blue rim. Аче As a source of water

30 used the strains Шν / мз and нϊν-ϊмтι ν / ιτв.

Suspension cages were placed in 24-hole panels; they were treated with various doses of the D-7 complex, with the ratio of D and metal 1: 1, 4: 9, 3:10, 0.5: 350: stings ΒICH. The infection rate was 0.0 ΙΤ CDS- _η

°° on the plug. - 33 -

After this culture, they incubated at 37 ° C in the atmosphere, which contained 5% of humidity and 98% for 5-7 days after the treatment was influenced. For the assessment of ωορмζρовъшпппус-

5 nytigena used a community analysis. Β The experiment was shown to have a large number of cases in% on the basis of the virus at the time of the loss of DZ-metal 3:10, died 25, lasted 69; at a ratio of 0.5: 350 - 35% and 81.2% of the living expenses

Ju gt; πρi sοοτnοshenii 1: 1000 - 70% and 84.3% zhiznesποsοb- us ^χ κleτοκ * πρi sοοτnοsheκii 1: 1000 - 100% and 86.2% viable sποsοbnyχ κleτοκ.

Ι ШИΜΕΡ 4. POSTING OF EXPERIMENT, AS IN EXAMPLE 3.

Susπenziyu κleτοκ οbρabaτyvali ρazlichnymi dοzami κοmπleκsa DΗΚ- # πρi τeχ same sοοτnοsheniyaχ κοmποnenτοv, chτο and πρimeρe I. Β eκsπeρimenτe ποκazanο, chτο κοliche- sτvο sintsiτiev in% οτ κοnτροlya πο viρusu πρi sοοτnοshenii

20 ДΗΚ: 4 ^' 3:10, 0,5: 350 was equal to zero, and the life of the cell was 81 and 90%; With a ratio of 1: 1000 - 68%, with 84.3% of life-saving cells and with a ratio of 1: 1100 - 100%, with 87.5% of life-saving cells.

25 APP 5.

Pοsτanοvκa eκsπeρimenτa κaκ in πρimeρe 3. Susπenziyu κleτοκ οbρabaτyvali ρazlichnymi dοzamζ κοmπleκsa DΗΚ-Sο πρi τeχ same sοοτnοsheniyaχ κοmποnenτοv, chτο in πρimeρe I. Β eκsπeρimenτe ποκazanο, chτο κοlichesτvο syn- 30% οτ tsiτiev in κοnτροlya πο viρusu πρi sοοτnοshenii DΗΚ-Sο

3:10 corresponds to 10% of the life of the carcass - 78.4%; With a ratio of 0.5: 350 - 15%, and with a life of 87.1%; At a ratio of 1: 100 - 66% with a life of 76.3% and at a ratio of 1: 1100, the life share was 82.1%. - 34 -

ПΡИ. 6.

Permanent experiment, such as in the case of I.

Susπenzshο κleτοκ οbρabaτyvali ρazlichnymi dοzami κοmπleκsa DΗΚ- Ρe, πρi τeχ same sοοτnοsheniyaχ κοmποnenτοv, chτο in πρimeρe I. Β eκsπeρimenτe ποκazanο, chτο κοlichesτvο sintsiτiev in οτ κοnτροlya πο viρusu πρi sοοτnοshenii DΗΚ-ϊe 3:10 0.5: 350 sοοτveτsτvueτ 0 In the case of cell life, respectively, 79.3% and 82.1%. With a supply of 1–1000, the number of syncites is 61% and the life of the cell is 81.7; At a ratio of 1: 1100 - 100%, and at a life of 84.5%.

ПΡИΜΕΡ 7.

The great diagnosis., The basic diagnosis is a histological system of the degree. Methodex was obtained at a dose of 500 mg / m2. On the 5th day of treatment, the incidence and occurrence of the disease were: the area of the patient was hyperamediated, there was a clear, sharp rash, which was painless. First of all, it is unlawful. The application was 0.25% delivered to the patient on the first day every 2 hours (8 times), 2-6 days 8 times a day, on day 7 - 6 times, 8 day - 3 times. In this case, the dynamics of clinical manifestations of the disease was as follows: on the 2nd day after treatment of mucous membranes, we noted the disappearance of pain, stabilization of acute manifestations ,. There is no other way around. After 3-4 days, there is no pain, a decrease in symptoms of food, the beginning of food intake on the 5th day - there is no pain, the disappearance of hyperemia, on the 6th day - a large period of food, mucous infections,

Treatment of the disease was carried out without the replacement of chemotherapy.

ПИИΜΕΡ 8.

Bolniy Κ. The main diagnosis is lymphoma. Received treatment: metathexate 500 mg / m2, range 2 mg / kg. - 35 -

On the 4th day after the start of the treatment, the manifestations of the disease became apparent. For convenience: the area of the hospital is hyperactive, accurate, and the effective rashes are located on the side of the cheek, decapacitor, and mouth. Palπatsiya slizisτοy and πρi- eιl πischi ρezκο bοl ^'eznenny.

5 At the beginning of the campaign, 0.5% of the distribution was paid off: on days 1-4 8 times a day (every 2-3 hours), on days 5-8 6 times a day. In this case, the dynamics of clinical manifestations of gs-igi-prostate was as follows ;: for 2 days after the treatment, a slight decrease in pain, for 3 days - the disappearance of pain,

^ Ο πρeκρaschenie κροvοτοchivοsτi, vοzmοzhen πρiem πshtsi, 4-5 day reduction giπeρemii slizisτοϊ on Day 8 -. Ποlnοs- τyο κuπiροvan προtsess. 9th day- mucous area of the mouth and a clean, pale-clear color, the drain and hyperemia were not detected. Treatment of gingivitis is without change

15 benefits.

ПΡИΜΕΡ 9.

Sick Κ. The main diagnosis is a simple leukemia. Treatment with a method of 500 mg / m2.

20 On 4 days after the onset of uterine arterial hypertension, the occurrence of complications arose.

On the other hand: slimy oral cavity and pharynx are smooth, clean, there are nice elements that are suitable for palpation. The mucous is painful. First of all _? οсο-

25 Bennas, foreign, tedious. After the start of the blasting, 1.5% of the sales were completed on the following basis: in the first 7 days - 8 times (every 2 hours), on days 8-9 - 6 times, on day 10 - 4 times. With this, the dynamics of medicamentous disease was as follows: on the 3rd day, they noted

30 illnesses, a considerable decrease in food intake, on the 4th day - the rate increased and the quantity and quantity decreased, on the 5-6th day, there was a slight increase in rainfall. 10 - day - full purchase of the process. The mucous area of the mouth and the throat is clean, well-groomed

35 colors, flow net. Treatment of an esophageal system is given without the substitution of chemotherapy. - 36 -

In general, the presented methods illustrate the efficacy of using a drug for treating medications for the treatment of patients

5 ПΡИΜΕΡ 10.

The receipt of a cosmetic circuit with a universal additive. After weighing the raw materials, they prepare the fat and water phases.

When a water volume of 0.1 is reached, it is 0.1 mm thick, and it heats up to 75-80 ° С. For commercial fatty acids, 0.5 lanlic, 1.0 times less than 0.5% stearin, 2.0 petroleum and 1.0 times 85% emulsified.

Next, mix the fat and water phases. If there are 15 conditions (temperature, intake, intake of water), add to the immediate mass of food and other products.

Received at the same time provides a one-for-one emulsion of white or light-colored colors with a sharp protection. Pharmacological research of the best product

20 showed the presence of negative anti-oxidative, protective, gross-productive, positive, positive gains. There are no annoying complications of ζ annoying. We will shine through the skin barrier.

25 ΙΙΡIΜΕΡ II :.

The production of the lotus, which is contained in the quality of biologic- garlic, is an additional supplement for sodium salt.

2.0 gel components are mixed with water and 0.20 ml thick. After the gel was obtained, a total of 30 injected 7.0 ethyl alcohol, 0.2 sweeter, 0.25 sodium salt, 0.2 odorant.

The forest is a gel-like liquid for hayfire. Experimental and clinical investigations of the investigations of the resultant losers have shown tax to the negative - 35 self-made, private, positive activities. Okay - 37 -

~ no other effects and allergic deformities have been identified.

ПΡИΜΕΡ ιг.

Obtaining a dental elixir, which is contained in a five-year standard business supplement - naturovuga sal dΗΚ.

The tank is loaded with 35.0 ethyl alcohol, 5.0 ml of distilled water, 0.25 DHS, 0.005 syringes (blue indigo and yellow cockpit) ζ 1.0 ou. All the ingredients are mixed. The resulting elixir delivers a rich liquid color with good smell and taste. Β Clinical studies have shown that the elixir has a direct, healing, antisynthetic action. It does not cause allergic and other complications. 15 In general, the presented examples illustrate the benefits of DI, and the use of it as a universal additive.

ПИИΜΕΡ 13.

20 After weighing the raw materials, prepare the fat and water phases. For the ingestion of an aqueous phase of water, 3.0 glycerol, or 1.2 pyrenephenylene glycol, 0.15 τ-ethanolamine is heated to 75-80 ° С. For livelihoods of a phase of 1.0 is a lantern of foodless, 1.0 is a part of midshipmen

25 disτζlliροvannyχ ζlζ mοnοsτeaρaτa glscheρina or sτeaρa- τa DEG sτeaρina κοsmeτichesκοgο 2.0, 2.0 πaρφyumeρnοgο oil ζlζ buτζlsτeaρaτa or izοιτροπilπalmiτατa or προπilmζρisτaτa, vοsκa πchelinοgο 1.0, 2.0 and the mixture mοnο- dieφiροv ποlieτilengliκοlya-400 and sτaaρaτa ρasπlavlyayuτ

30 to 80-85 ° С. They mix the liquid and the aqueous phases, add 3.33 ingredients, 0.25 sodium salt, 0.4 odorants.

Obtained at the same time provides a uniform, dense mass of white flowers, and takes advantage of the stockpile. Pharmaceutical research conducted in the Czech Republic revealed the presence of

3 with its hostile, defensive, anti-oxidative, anti-inflammatory properties. - 38 -

No complications have been identified. Good προ- doesn’t go through a dry bar.

ПΡИΜΞΡ 1Ц.

5 Weigh raw materials. D. for industrial water phase, 3, 0 glycerin or 1,2 πpopilenglikol, 0.2 sulphate acid and polysynthetate or sulphate acid. erpa acronyms heat up to 75-80 ° С. For fat burning

- ^'• 0 lanοlina οκsieτζlζροvannοgο 0.5, 1.0 mοnοglscheρvdοv dis- τιιlliροvannyχ ζlζ mοnοsτeaρaτa glscheρina ζlζ sτeaρaτa DSG, 1.0 vοsκa πchelζnοgο, 3.0 πaρφømeρnοgο oil or buτil- sτeaρaτa or izοπροπζlπalmζnaτa 3.0 mixture mοnø- and diethylene glycol-400 ζ stearez, 2.0 mixture of mono-5 and diesel acid-polysylic acid-400,

0.5 of the fraction of the sigm -.- S. (§ heat up to 80-85 ° C. Then mix the aqueous and fat phases. Add the 3.33 part of the mixture, while the temperature is 0.25 in. 2. Pοluchenny κρem πρedsτavlyaeτ sοbοy οdnοροd- hydrochloric emulsion or belοgο sveτlο-κρemοvοgο tsveτa with πρiyaτ- nym zaπaχοm. Φaρmaκοlοgζchesκζe ζssledοvaniya ποluchennοgο κρema ποκazali presence of negο anτiοκsvdanτιshχ, φοτοzaschi- τnyχ, gidρaτanτnyχ, προliφeρaτζvnyχ, προτivοvοsπaliτel- nyχ svοysτv. Αlleρgichesκiχ οslοzhneny and ρazdρazhayuschegο 5 actions not identified. We ем χ ρ ρ π π through the barrier.

- 39 - I. Slanchak S. С. , On the other hand. Евπев, "Βыιпая ιдκο- лε, 1982, pp. 99-204.

2. Ge ι а а Ε Ε Ι Ι , Complications of chemo-mono-therapies of malignant diseases. ...., ... one

5 pin, 1982, p. 9C-Ι42.

3. YOUR OWNERS , Indirect components. ϊl. , Ieditsina, 1985, with. 172, τ. 2.

4. REGULATORY MEDICINES OF RUSSIA 1.1. , Anterfarash, 1933, p. 603.

Yu 5. Μ. Βgee ι 5. Зϊеηа Гgeак-Іιгоиβη ϊ ^η Ь ο • • • ι ι ега;; aη

οϊ "SΜ-S5Ρ Ηοuaϊ Zοsϊe-Ly οG Μeάϊsχηe Zegνϊsez Ι ^{η *} L. Sοschρ.ezz aηά Zutροzϊiιη Zegϊez Ν 170. ρ. 491-6.

6. Ρ. Ρиρ-bа е_ аϊϊ. Βοηе Μаггον. Τга ^η зρ1а ^{η *} Л-15 -Ыοη 1992, Ν 9, ρ. 491-492.

7. Ρ. Εϋкοηеη, υ. Zaagχηеη, Μеά ^χ саϊ а ^η ά Ρеάχа.гχс Οηеοϊοе; 1992, Ν 20, ρ. 489-496.

8. Belous Δ.Μ. , Exogenous Nucleic Acids ζ Process Constituent Processes, 1.1. , Μedζshscha,

20 1974, p. 126-140.

9. KNOWLEDGE Β.Β., Acidic acids and their therapeutic use. Medical case, 1981, $ 8, p. ΙΙ4-ΙΙ8.

10. Soboleva EP , The use of ДΗΚ πρζ гζτοπ- 25 ншι, ЫЗΕЭΕЙ мейолосаномом. Bull. , eksπ. biol. and honey., 1976, τ. 81, 4, p. 409-411.

11. Sa_ιg ^* Lοη S., Zh. az a saggϊeg Gog ο ϊ ϊ ϊ ϊ ^{• • • • • • • •} ге а а

Heiket ^χ a. 1asta1 ^; ο1θ £ ϊа, 1991 ν.76, ΕГ4, ρ. 99.

30 12. I.E. Barking, L.S. Pilanova. Pharmaceutical AIDS. Pharmac. and τοκxy. 1989, τ. 52,.!> 3, p. Ι0Ι-ΙΙ0.

13. ΜΚand ⁵ Α 61 Κ 3170, Φρ station, hare. _. .52643558, οπ publ. 08/31/90. 35 14. Ρ.Β. Petr, Α.Μ. Borysova. Modern problems of diagnosis, treatment and лем ρ ο φ Ι лечения Ι Ι π. , Immunology, 1987, _ ^• ! .3, p. 5-10. - 40 -

15. Ρгаηζа Β. S-Gagas.egxζaΙ.χο-1 ο: G seϊϊiϊag ρгг--еχχ а аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз аз есес есесес ес есесес есесес ес есесес есесес ес ес ес ес ес ес ес ес ес есесес есесес get-get-you-go. Za.ige, 1987, ν. 330, ρ. 391-395.

16. ΜΚand ⁴ Λ6Ι Κ 9/66, 37/22, ΡСΤ (\ Υ0); 589/02733, οπ publ. 04/06/89.

17. ЛΟΓΙΙΗΟΕ Α.S. and dρ. Drugs for the treatment of nucleic acids with an acute gastric ulcer.

10 bul. eksp. biol. and honey. , 1991, .'37, p. 59-60.

18. Kolpenson I.I. The survey of the day, Russia, 1988., p. ΙΙ-49.

19. Consumer goods (manual) prior to general ed. Η. ΜζχаплоΕа, Hedivshchyna, 1984, p. 31-44.

15 20. ЗϊΕϊΕϊΕτные - - - - --Β - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. 20. Zy 20.tτnye - Models for the study of the KICH and SUSCHA: memorandum of the meeting Bull. ΒΟЗ 1988, τ. 66.35, p. 20-36.

21. Community Immunodeficiency (AIDS): The outcomes and recommendations of the Council of Ministers. Bull.

20 ΒΟЗ. 1985, τ. 63, I '4, p. 18-19.

22. Bazhanov Η.Η. Stomatology. , Μ., 1984

23. BοροΕSky Ε.Β. ζ dρ. Terrestrial facility. , Μ. , Russia, 1973, p. 318-323.

24. Medicinal disease. Before ρed. The city of ηashchinae-25, St. Petersburg, 1973, p. ΙΙЗ-Ι24.

^• '25. ΚοροлёΕа Η.Б. Practical and clinical assessment of the quality of the test. The Presidents of the All-Union Congress of the Decem / Congress. , Μ. 1979

26. Coastal Η.Μ. , Ле ле ле ο л л л (((с (π ρ κ дум дум "" "" "", ",, дум,,), 1986, p. 291.

27. ΚοροлёΕа Η.Б. Practical and clinical evaluations of the evaluation of environmental impairment. The All-Union Congress of Dermatologists, Μ. 1979.

28. ΚοροлёΕа Η.Б. Scientific Scientist κ

35 'for the application of biologically active substances

Ме on the spot. Gestures ζκ dermatology. and venus. 1977, I 6, p. 8-12. - 41 -

29. Sudrae Α ... Ϊ. Eleκτροιleτριg.esκsh1 meτοd pssle- dοvanπya πaρagleτροΕ gidρaτanτnyχ SΕΟΙ ^'ΪSΤΕ And miκροπiρ- κulyatsπi κο :::: ι. Diss Cand. unit nεuκ, Ι.Ι., 1990

30. ΚοροлёΕа Η.Б. with sοaΕΤορ. EXPERTS.VENTAL- 5 Basic study of bi-large-scale economics and ζχ ζ ρ ρ ла ла........... Τρudy Zses. the congress of the city. , Μ. , 979g.

Claims

- 42 -

ΦΟΕ.ϊУΜ И30ΕΡ__Τ__ΕП_Я I. СΟСΤЭΕ нιιзкоыоелеϊ-улярній ДΕПС, received :. from the old fish, excluding no more than: l C0? _ at the weight of the salt of DGP. with a molecular nassost no more than 5.0 or more 12.0 CDs and more than a minimum of 372., no more than 1.5% by weight of protein, no more than Τ7 π worth of nonsense.

2. Composition πο π. I, that is, with the result that the indicated ДС is found in combination with polished metals.

3. СΟСΤЭΕ πο π. 2, that is, that metals are selected from the group, excluding

Ζη, Сο, Ι.1, He, 18., Ρ-.

4. ΦaρmatseΕΤϊ _'ϊchesκy πρeπaρaτ, sοdeρ "aιsch-π-ϊ Ε κaches- 15 ΤΕe aκτιϊΕΗθgο agenτa nριzκοmοleκulyaρnuyu DΗΕS of mοlοκ οseτροΕyχ ρab πο π. I in effect, in combination with pharmaceutically acceptable additives, spacers or amps.

5. Pharmaceuticals containing 20 а- 20 чес чес чес чес чес чес чес 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 Φ Φ Φ Ε а Ε Ε 5. Pharmaceutical agent containing а Ε чес чес Ε чес Ε ΚΤ ΚΤ 20 Ο 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20. 2 in ef- fective quantity in combination with pharmaceutical additives, distributors or fillers. 25 • -6. Pharmaceuticals πο π. 4, ο τ l ι. - that is, that is located in the factory.

7. Pharmaceuticals. 6, which means that injections are used. 30 8. Pharmaceuticals. 6, which is different from what is needed.

9. Pharmaceutical. π е π π π. 7, in fact, moreover, that a preferable content of the indicated CCD is 0.5 to 2.5%. 35 ^'• 10. Pharmaceutical preparation πο π. C, ο τ l and - - 43 -

- Part I: _1 I, which is a favorable affiliates of 0.2% to 2%.

11. Pharmacy-ιscheskιϊ-- π е е π π π π. 5, ο τ L n - h and w and u π i with τen, chτο sοοτnοshenie DSHS and ποliΕalen- 5 τnyχ neτallοΕ sοsτavlyaeτ οτ 1: 1 dο 1: 1000.

12. Composed πο π. I, r and h and uchshch and G: with that, that is extended in the form of a cosmetic medium

In combination with the pharmaceuticals, the non-dusted were found, for example, in additions. 10 13. Composition πο π. 12, and so on, that provides a special dental elixir or toothpaste.

14. СΟСΤЭΕ πο π. 12, for the sake of the title, that is, it is a good forest. 15 15. Composed πο π. 12, which is the same as that found in the cream or gel.

16. The method of separating low-income DUZH from the small fish community, which involves the homogenization of the community, is small in the city,

20 the deletion of DZh with detergents, the denaturation and separation of the protein following the degradation of DZh, the separation of it from the specifics and the separation of the food, this is

25 Lisa ιι / ilp ultrafiltration, and the target product is waiting for you from the party from the United States for ethical sirp.

17. The case of πο π. 16, which is easy to use, so that the ultimate in filtering

30 are pressure and temperature equal to 20-24 ° С and pressure of 2-3 kg / cm ² .

10. Method πο π. 16, with the possibility that elec- trialysis is feasible at temperature equal to 5-20 ° C, the density is 1.0-1.5 Α / dm.