WO1996008578A3 - Improved procedure for the solid phase synthesis of oligonucleotides - Google Patents

Improved procedure for the solid phase synthesis of oligonucleotides Download PDF

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Publication number
WO1996008578A3
WO1996008578A3 PCT/US1995/011302 US9511302W WO9608578A3 WO 1996008578 A3 WO1996008578 A3 WO 1996008578A3 US 9511302 W US9511302 W US 9511302W WO 9608578 A3 WO9608578 A3 WO 9608578A3
Authority
WO
WIPO (PCT)
Prior art keywords
solid phase
capping
oligonucleotides
phase synthesis
improved procedure
Prior art date
Application number
PCT/US1995/011302
Other languages
French (fr)
Other versions
WO1996008578A2 (en
Inventor
Radhakrishnan P Iyer
Sudhir Agrawal
Original Assignee
Hybridon Inc
Radhakrishnan P Iyer
Sudhir Agrawal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybridon Inc, Radhakrishnan P Iyer, Sudhir Agrawal filed Critical Hybridon Inc
Priority to AU35469/95A priority Critical patent/AU3546995A/en
Priority to EP95932418A priority patent/EP0779892A2/en
Publication of WO1996008578A2 publication Critical patent/WO1996008578A2/en
Publication of WO1996008578A3 publication Critical patent/WO1996008578A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)

Abstract

An improved method of solid phase oligonucleotide synthesis is presented. The method results in lesser contamination of the final product with N-1, N-2, and other failure sequences. The method comprises employing a second capping step after initial loading and capping of the solid support and before addition of the second nucleoside. Further diminution in the N-1 oligonucleotide content can be realized when this second capping stepped is used in combination with loading the support to higher densities than have previously been deemed acceptable and by cleaving the full length oligonucleotide from the solid support using mildly basic conditions.
PCT/US1995/011302 1994-09-06 1995-09-05 Improved procedure for the solid phase synthesis of oligonucleotides WO1996008578A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU35469/95A AU3546995A (en) 1994-09-06 1995-09-05 Improved procedure for the solid phase synthesis of oligonucleotides
EP95932418A EP0779892A2 (en) 1994-09-06 1995-09-05 Improved procedure for the solid phase synthesis of oligonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30133794A 1994-09-06 1994-09-06
US08/301,337 1994-09-06

Publications (2)

Publication Number Publication Date
WO1996008578A2 WO1996008578A2 (en) 1996-03-21
WO1996008578A3 true WO1996008578A3 (en) 1996-05-30

Family

ID=23162922

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/011302 WO1996008578A2 (en) 1994-09-06 1995-09-05 Improved procedure for the solid phase synthesis of oligonucleotides

Country Status (4)

Country Link
EP (1) EP0779892A2 (en)
AU (1) AU3546995A (en)
CA (1) CA2199246A1 (en)
WO (1) WO1996008578A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0040099A1 (en) * 1980-05-14 1981-11-18 ens BIO LOGICALS INC. Polynucleotide synthesis
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
EP0040099A1 (en) * 1980-05-14 1981-11-18 ens BIO LOGICALS INC. Polynucleotide synthesis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
M.J. DAMHA ET AL.: "An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis", NUCLEIC ACIDS RES., vol. 18, pages 3813 - 21 *
R.P. IYER AND S. AGRAWAL: "Improved procedure for the reduction of N-1 content in synthetic oligonucleotides", NUCLEOSIDES, NUCLEOTIDES, vol. 14, pages 1349 - 57 *

Also Published As

Publication number Publication date
CA2199246A1 (en) 1996-03-21
WO1996008578A2 (en) 1996-03-21
EP0779892A2 (en) 1997-06-25
AU3546995A (en) 1996-03-29

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