WO1996040070A1 - Fragrances and flavorants - Google Patents
Fragrances and flavorants Download PDFInfo
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- WO1996040070A1 WO1996040070A1 PCT/US1996/010183 US9610183W WO9640070A1 WO 1996040070 A1 WO1996040070 A1 WO 1996040070A1 US 9610183 W US9610183 W US 9610183W WO 9640070 A1 WO9640070 A1 WO 9640070A1
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- composition
- active agent
- proteinoid
- microsphere
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/70—Fixation, conservation, or encapsulation of flavouring agents
- A23L27/72—Encapsulation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/015—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone
- A61L9/04—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone using substances evaporated in the air without heating
- A61L9/042—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone using substances evaporated in the air without heating with the help of a macromolecular compound as a carrier or diluent
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q13/00—Formulations or additives for perfume preparations
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
Definitions
- compositions which include fragrances or flavorants as active agents and to the delivery of such agents.
- These compositions are in the form of proteinoid or modified hydrolyzable vegetable protein microspheres which can be adapted to release the active agent in specific pH ranges. Methods for the preparation and for the use of such compositions are also disclosed.
- Proteinoid microspheres have been described for encapsulating pharmaceuticals for oral deliver. (Steiner, et al., U.S. Patent No. 4,925,673). However, it has now been discovered that other specific active agents can be delivered with proteinoid microspheres.
- Figure 1 is a graphic illustration of the head space concentrations of clove oil at various pH's from clove oil containing proteinoid microspheres in comparison with clove oil or clove oil in combination with proteinoids that are not in microsphere form.
- Figure 2 is a graphic illustration of the head space concentrations of the components of clove oil at various pH's from clove oil containing proteinoid microspheres in comparison with clove oil or clove oil in the presence of proteinoids that are not in microsphere form.
- compositions useful in the delivery of fragrances and flavorant active agents, and particularly vaporous fragrances and flavorants are provided. These compositions comprise a microsphere which comprises (a) the active agent; and (b) (i) a proteinoid, (ii) a modified hydrolyzed vegetable protein wherein the protein is modified with an amino reactive agent, or (iii) a combination thereof.
- compositions wherein the active agent is mixed with the proteinoid of hydrolyzed vegetable protein solution and the proteinoid or modified hydrolyzed vegetable protein is precipitated out of the solution, thereby forming a microsphere containing the active agent.
- the active agent is applied to a substrate.
- the present invention is suited to the delivery of active agents and fragrances or flavorants which optionally may be in a vaporous form either before or after incorporation into a microsphere.
- the present compositions incorporate readily available or easy to prepare, inexpensive starting materials.
- the formulation methods of the present invention are cost effective for preparing and isolating these compositions, are simple to perform, and are amenable to industrial scale for commercial production.
- the active agents of the present invention include flavorants and fragrances.
- Flavorants are compounds or compositions that either increase or enhance an existing taste or that impart a specific taste.
- Fragrances are compounds or compositions that either increase or enhance an existing smell or odor or that impart a specific agreeable smell or odor.
- These fragrances and flavorants may be solids, liquids, vapors, or any combination thereof. Furthermore, they may completely or partially change state before being incorporated into a microsphere, while incorporated in a microsphere, or after being partially or completely released from a microsphere.
- Non-limiting examples of flavorants and fragrances are clove oil.
- Vaporous active agents are those that are at least partially vaporous before incorporation in a microsphere and/or while incorporated in a microsphere.
- compositions of the present invention may include one or more active agents.
- Proteinoids are artificial polymers of amino acids.
- An amino acid is any carboxylic acid having at least one free amine group and includes naturally occurring and synthetic amino acids.
- Amino acids suitable for use herein include naturally occurring and synthetic amino acids as well as ⁇ - and non ⁇ -amino acids. Representative, but not limiting, amino acids suitable for use in the present invention are generally of the formula
- R 1 is hydrogen, C 1 -C 4 alkyl, or C 2 -C 4 alkenyl
- R 2 is C 1 -C 24 alkyl, C 2 -C 24 alkenyl, C 3 -C 10 cycloalkyl, C 3 -C 10 cycloalkenyl, phenyl, naphthyl, (C 1 -C 10 alkyl) phenyl, (C 2 -C 10 alkenyl) phenyl, (C 1 -C 10 alkyl) naphthyl, (C 2 -C 10 alkenyl) naphthyl, phenyl (C 1 -C 10 alkyl), phenyl (C 2 -C 10 alkenyl), naphthyl (C 1 -C 10 alkyl), or naphthyl (C 2 -C 10 alkenyl);
- R 2 being optionally substituted with C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 1 -
- R 2 being optionally interrupted by oxygen, nitrogen, sulfur, or any combination thereof;
- R 3 is hydrogen, C 1 -C 4 alkyl, or C 2 -C 4 alkenyl.
- the preferred amino acids for use in the present invention are ⁇ - amino acids, and most preferably are naturally occurring ⁇ -amino acids.
- Many amino acids and amino acid esters are readily available from a number of commercial sources such as Aldrich Chemical Co. (Milwaukee, Wl, USA); Sigma Chemical Co. (St. Louis, MO, USA); and Fluka Chemical Corp. (Ronkonkoma, NY, USA).
- Preferred naturally occurring amino acids for use in the present invention as amino acids or components of a peptide are alanine, arginine, asparagine, aspartic acid, citrulline, cysteine, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, omithine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, hydroxy proline, ⁇ -carboxyglutamate, phenylglycine, or O-phosphoserine.
- the preferred amino acids are arginine, leucine, lysine, phenylalanine, tyrosine, tryptophan, valine, and phenylglycine.
- Non-limiting examples of non-naturally occurring amino acids for use in the present invention are ⁇ -alanine, ⁇ -amino butyric acid, ⁇ -amino butyric acid, ⁇ -(aminophenyl) butyric acid, ⁇ -amino isobutyric acid, citrulline, ⁇ -amino caproic acid, 7-amino heptanoic acid, ⁇ -aspartic acid, aminobenzoic acid, aminophenyl acetic acid, aminophenyl butyric acid, ⁇ -glutamic acid, cysteine, ⁇ - lysine, methionine sulfone, norleucine, norvaline, ornithine, d-ornithine, p- nitro-phenylalanine, hydroxy proline, 1 ,2,3,4,-tetrahydroisoquinoline-3-carboxylic acid, and thioproline.
- the proteinoids useful herein preferably are prepared from mixed amino acids.
- Preferred proteinoids are condensation polymers, and most preferably, are thermal condensation polymers. These polymers may be directed or random polymers. Proteinoids can be linear, branched, or cyclical, and certain proteinoids can be units of other linear, branched, or cyclical proteinoids.
- Diketopiperazines are six member ring compounds.
- the ring includes two nitrogen atoms and is substituted at two carbons with two oxygen atoms.
- the carbonyl groups are at the 1 and 4 ring positions. These rings can be optionally, and most often are, further substituted.
- Diketopiperazine ring systems may be generated during thermal polymerization or condensation of amino acids or amino acid derivatives. (Gyore, J; Ecet M. Proceedings Fourth ICTA (Thermal Analysis), 1974, 2, 387- 394 (1974)). These six membered ring systems were presumably generated by intra-molecular cyclization of the dimer prior to further chain growth or directly from a linear peptide (Reddy, A.V., Int. J. Peptide Protein Res., 40, 472-476 (1992); Mazurov, A.A. et al., Int. J. Peptide Protein Res., 42, 14-19 (1993)).
- Diketopiperazines can also be formed by cyclodimerization of amino acid ester derivatives as described by Katchalski et al., J. Amer. Chem. Soc, 68, 879-880 (1946), by cyclization of dipeptide ester derivatives, or by thermal dehydration of amino acid derivatives and high boiling solvents as described by Kopple et al., J. Org. Chem., 33 (2), 862-864 (1968).
- Diketopiperazines typically are formed from ⁇ -amino acids.
- the ⁇ -amino acids of which the diketopiperazines are derived are glutamic acid, aspartic acid, tyrosine, phenylalanine, and optical isomers of any of the foregoing.
- R 4 , R 5 , R 6 , and R 7 independently are hydrogen, C 1 -C 24 alkyl, C 1 -C 24 alkenyl, phenyl, naphthyl, (C 1 -C 10 alkyl)phenyl, (C 1 -C 10 alkenyl)phenyl, (C 1 -C 10 alkyl)naphthyl, (C 1 - C 10 alkenyl)naphthyl, phenyl (C 1 -C 10 alkyl), phenyl(C 1 -C 10 alkenyl), naphthyl (C 1 -C 10 alkyl), and naphthyl (C 1 -C 10 alkenyl); any of R 4 , R 5 , R 6 , and R 7 independently may optionally be substituted with C 1 -C 4 alkyl, C 1 -C 4 alkenyl, C 1 -C 4 alkoxy, -OH, -SH, and -
- the phenyl or naphthyl groups may optionally be substituted.
- Suitable, but non-limiting, examples of substituents are C 1 -C 8 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkoxy, -OH, -SH, or CO 2 R 9 wherein R 9 is hydrogen, C 1 -C 6 alkyl, or C 1 -C 6 alkenyl.
- R 6 and R 7 independently are hydrogen, C 1 -C 4 alkyl or C 1 - C 4 alkenyl.
- diketopiperazines which are preferred complexing perturbants. These diketopiperazines include the unsubstituted diketopiperazine in which R 4 , R 5 , R 6 , and R 7 are hydrogen, and diketopiperazines which are substituted at one or both of the nitrogen atoms in the ring, i.e. mono or di-N-substituted. Special mention is made of the N-substituted diketopiperazine wherein one or both of the nitrogen atoms is substituted with a methyl group.
- R 10 and R 11 independently are hydrogen, C 1 -C 24 alkyl, C 1 -C 24 alkenyl, phenyl, naphthyl, (C 1 -C 10 alkyl)phenyl, (C 1 -C 10 alkenyl)phenyl, (C 1 -C 10 alkyl)naphthyl, (C 1 -C 10 alkenyl) naphthyl, phenyl (C 1 -C 10 alkyl), phenyl(C 1 -C 10 alkenyl), naphthyl (C 1 -C 10 alkyl), and naphthyl (C 1 -C 10 alkenyl); but both R 10 and R 11 can not be hydrogen; either or both R 10 or R 11 independently may optionally be substituted with C 1 -C 4 alkyl, C 1 -C 4 alkenyl, C 1 -C 4 alkoxy, -OH, -SH, and - CO 2 R 12 or any combination thereof;
- the phenyl or naphthyl groups may optionally be substituted.
- substituents are C 1 -C 6 alkyl, C 1 -C 6 alkenyl, C 1 -C 6 alkoxy, -OH, -SH, or CO 2 R 13 wherein R 13 is hydrogen, C 1 -C 6 alkyl, or C 1 -C 6 alkenyl.
- R 10 or R 11 When one of R 10 or R 11 is hydrogen, the diketopiperazine is mono-carbon-(C)-substituted. When neither R 10 nor R 11 is hydrogen, the diketopiperazine is di-carbon-(C)-substituted.
- R 10 , R 11 , or both R 10 and R 11 contain at least one functional group, a functional group being a non-hydrocarbon portion responsible for characteristic reactions of the molecule.
- Simple functional groups are heteroatoms including, but not limited to halogens, oxygen, sulfur, nitrogen, and the like, attached to, the carbon of an alkyl group by a single or multiple bond.
- Other functional groups include, but are not limited to, for example, hydroxyl groups, carboxyl groups, amide groups, amine groups, substituted amine groups, and the like.
- Preferred diketopiperazines are those which are substituted at one or two of the carbons of the ring with a functional group that includes at least one carboxyl functionality.
- Modified hydrolyzed vegetable protein is prepared from hydrolyzed vegetable protein.
- Hydrolyzed vegetable protein is a product which is derived from defatted vegetable meal.
- acid or enzyme hydrolyzed vegetable proteins are useful.
- the vegetable proteins generally contain titratable carboxylic acid groups (COOH) ranging from about 3 to about 8 milliequivalents/g, preferably from about 4 to about 6 milliequivalents/g, and total free amino groups (NH 2 ) ranging from about 3 to about 9 milliequivalents/g, preferably ranging from about 4 to about 7 milliequivalents/g NH 2 .
- the molecular weight of the hydrolyzed vegetable protein ranges from about 100 daltons to about 2000 Daltons, and preferably from about 200 to about 500 daltons.
- Hydrolyzed vegetable protein is available from a variety of commercial sources, such as, for example, Ajinomoto USA, Inc. (Teaneck, NJ); Central Soya Co., Inc. (Fort Wayne, IN); Champlain Industries, Inc. (Clifton, NJ,); Archer Daniels Midland (Decatur, IL), A.E. Staley Company, Gunther Products Division, (Decatur, IL), and additional companies listed in "Food Engineering Master", an annual publication of Chilton Co., Radnor, PA.
- a preferred hydrolyzed vegetable protein in practicing this invention is available from Ajinomoto USA under the tradename AJI-EKI. This product is an acid hydrolyzed liquid soybean protein which is derived from defatted soybean meal.
- Other preferred hydrolyzed soy proteins include PROFAM 781 , available from Archer Daniels Midland and PTOT 1550 and MIR-A-FOAM 100 available from A.E. Staley, Gunther Products division.
- a dried protein extract of the hydrolyzed vegetable protein solution may be used to prepare the modified hydrolyzed vegetable protein of the invention.
- the dried protein extract is preparable by extracting the hydrolyzed vegetable protein solution with a suitable solvent, e.g., methanol, followed by evaporating the solvent extract.
- the hydrolyzed vegetable protein is modified by an amine reactive agent.
- the hydrolyzed vegetable protein is modified by acylating or sulfonating at least one free amine group, with an acylating or sulfonating agent which reacts with at least one of the free amine groups present.
- Suitable, but non-limiting, examples of acylating or sulfonating agents useful for preparing the modified hydrolyzed vegetable protein emulsifiers of the present invention include acylating and sulfonating agents having the formula: wherein R 14 is alkyl or alkenyl, preferably having from 1 to 20 carbon atoms, or aromatic preferably having from 6 to 20 carbon atoms and n is 1 or 2.
- the R 14 group can be substituted or unsubstituted,
- the preferred substituents include C 1 to C 4 alkyl, C 1 to C 4 alkenyl, C 1 to C 4 alkoxy, CO 2 R 15 wherein R 15 is hydrogen, C 1 to C 4 alkyl or C 1 to C 4 alkenyl.
- R 14 is methyl, ethyl, phenyl, benzyl or naphthyl. More preferably, R 14 is phenyl, or acetyl.
- X is a leaving group. In a reaction in which the substrate molecule becomes cleaved, part of it (the part not containing the carbon) is usually called the leaving group. See Advanced Organic Chemistry,
- Typical leaving groups include, but are not limited to, halogens such as chlorine, bromine and iodine.
- acylating and sulfonating agents for modifying hydrolyzed vegetable protein include, but are not limited to, acyl halides, such as, for example, acetyl chloride, propyl chloride, benzoyl chloride, phthaloyl chloride, hexahydrophthaloyl chloride, tetrahydrophthaloyl chloride, cyclohexanoyl chloride, sebacoyl chloride, hippuryl chloride and the like; sulfonyl halides, such as, for example, benzene sulfonyl chloride, acetylsulfanilyl chloride, and the like; anhydrides, such as, for example, acetic anhydride, propyl anhydride, benzoic anhydride, maleic anhydride, phthalic anhydride, tetrahydrophthalic anhydride, hexahydrophthalic anhydride, hippuric anhydride and the like.
- the preferred acylating and sulfonating agents are benzoyl chloride, benzene sulfonyl chloride, cyclohexanoyl chloride, phthalic anhydride, tetrahydrophthalic anhydride, and hexahydrophthalic anhydride.
- the hydrolyzed vegetable protein is typically modified by first dissolving it in aqueous alkaline solution of a metal hydroxide, e.g., sodium or potassium hydroxide, and heating at the solution to a temperature ranging from about 50°C to about 70°C, preferably from about 50°C to about 60°C, for a period ranging from about 10 minutes to about 40 minutes, preferably about 15 minutes.
- a metal hydroxide e.g., sodium or potassium hydroxide
- the amount of alkali employed per mmole of titratable NH 2 in the hydrolyzed vegetable protein generally ranges from about 2 to about 3 mmole, and preferably from about 2.2 to about 2.5 mmole.
- the pH of the solution is generally maintained from about 8 to about 13, preferably ranging from about 9 to about 10.
- acylating or sulfonating agent is added to the reaction mixture.
- the amount of acylating or sulfonating agent in relation to the quantity of hydrolyzed vegetable protein employed is based on the equivalents of total free NH 2 in the hydrolyzed vegetable protein.
- from about 0.3 to about 1.2 equivalents of acylating or sulfonating agent are used for each molar equivalent of total NH 2 groups in the hydrolyzed vegetable protein, and preferably from about 0.6 to about 1.0 equivalent of acylating or sulfonating agent for each molar equivalent of groups NH 2 groups in the hydrolyzed vegetable protein.
- the modified hydrolyzed vegetable protein is then recovered from the reaction mixture using standard techniques, such as, for example, precipitation with dilute acid and filtration of the precipitate. See also, PCT Publication No. WO94/14420 (July 7, 1994). Microspheres
- the microspheres of the present invention can generally be of a matrix form or the microcapsule form.
- the matrix form includes both hollow matrix spheres in which the proteinoid forms a matrix shell around a hollow center, and the active agent is distributed throughout the matrix, and a solid matrix sphere in which the proteinoid forms a circle matrix continuing in which the active agent is distributed.
- the microcapsule form is one in which the encapsulated active agent either is in a vapor solution, a solid state, or any combination thereof, with the carrier forming a shell around the encapsulated material.
- the microcapsule form is the form most often taken by the self assembly with the proteinoids of the present invention.
- the microspheres can also have the flavor or fragrance adsorbed thereon.
- the microspheres are preferably non-porous.
- Microspheres can be prepared by dissolving the carrier, i.e., proteinoid or modified hydrolyzed vegetable protein in an appropriate solvent and then stimulating self assembly by contacting the carrier solution with a precipitator. Solubility of the carrier can be regulated by the selection of the appropriate amino acid.
- the carrier, and therefore, the microsphere compositions of the present invention can be pH adapted to be selectively soluble in specific acidic, basic, or neutral pH ranges.
- compositions which are targeted to an acidic environment can be made selectively soluble at specific ranges of acidic pH.
- These compositions are prepared with an acid-soluble carrier.
- the acid-soluble carrier exists largely in the cation form in at least a portion of the pH range from about 1 to about 6.8.
- the carrier is largely unprotonated and insoluble in water. Therefore, the carrier could self assemble to microspheres at a different acidic pH or at a basic or neutral pH, and the active agent in the composition would not be released until the carrier solubilizes upon encountering the selected acidic pH.
- compositions which are to be targeted to an alkaline environment can be made selectively soluble at specific ranges of alkaline pH.
- These compositions are prepared with a base-soluble carrier.
- the base-soluble carrier exists largely in an anionic form in at least a portion of the pH range of from . about 7.2 to about 1 1.
- the carrier is largely protonated and insoluble in water. Therefore, the carrier could self assemble to microspheres at a different basic pH or acidic or neutral pH, and the active agent in the composition would not be released until the carrier solubilizes upon encountering the selected basic pH.
- compositions which are targeted to a neutral environment can be made selectively soluble at neutral pH.
- These compositions are prepared with a neutral-soluble carrier.
- the neutral-soluble carrier exists largely in a neutral form at neutral pH, i,e. from about 6.8 to about 7.2. However, above or below this range, the carrier is insoluble in water. Therefore, the carrier could self assemble to microspheres at acidic or basic pH, and the active agent in the composition would not be released until the carrier solubilizes upon encountering a neutral pH.
- the final solution can contain from about 10 mg to about 2000 mg of carrier per ml of solution, preferably between about 75 to about 500 mg of carrier per ml of solution, and most preferably from about 75 to about 200 mg per ml.
- the mixture is heated to a temperature between about 20° C and about 60° C, preferably about 40°C, until the carrier dissolves. Particulates remaining in the solution may be filtered out by conventional means such as gravity filtration over filter paper.
- the carrier solution usually is maintained at the elevated temperature and is mixed with the non-biologically active agent and a precipitator, for example, an acid solution such as, for example, aqueous acetic or citric acid at a concentration ranging from about 1 N to about 3N for acid insoluble carriers, a basic solution for base insoluble carriers, and a neutralizing solution for neutral insoluble carriers.
- a precipitator for example, an acid solution such as, for example, aqueous acetic or citric acid at a concentration ranging from about 1 N to about 3N for acid insoluble carriers, a basic solution for base insoluble carriers, and a neutralizing solution for neutral insoluble carriers.
- the active agent can be mixed with the precipitating solution or can be used separately.
- the resultant mixture is maintained for a period of time sufficient for microsphere formation as observed by light microscopy.
- the precipitating solution is added to the carrier solution, the carrier solution can be added to the precipitating solution as well.
- the solutions above may optionally contain additives such as stabilizing additives
- the presence of such additives promotes the stability and dispersability of the non-biologically active agent in solution.
- the stabilizing additives may be employed at a concentration ranging between about 0.1 and 5% (w/v), preferably about 0.5% (w/v).
- Suitable, but non-limiting examples of stabilizing additives include buffer salts, gum acacia, gelatin, methyl cellulose, polyethylene glycol, and polylysine.
- the preferred stabilizing agents are gum acacia, gelatin, and methyl cellulose.
- the amount of active agent that may be encapsulated by the microsphere is dependent upon a number of factors which include the concentration of active agent in the microsphere or precipitator solution as well as the affinity of the active agent for the proteinoid.
- concentration of the active agent in the final formulation also will vary depending on the required amount for treatment. When necessary, the exact concentration can be determined by, for example, reverse phase HPLC analysis.
- microspheres of the present invention can also aid in providing efficient delivery of the active agent.
- microspheres of the present invention will have a diameter of less than 10 ⁇ m , preferably in the range of from about 0.1 ⁇ m to about 10 ⁇ m, and most preferably in the range of from 0.2 ⁇ m to about 10 ⁇ m.
- the size of the microspheres containing an active agent can be controlled by manipulating a variety of physical or chemical parameters, such as the pH, osmolarity, ionic strength of the encapsulating solution, or size of the ions in solution, and/or by the choice of the precipitator used in the microsphere forming and loading process.
- compositions of the present invention may be formulated into unit forms by the addition of one or more excipient(s), diluent(s), disintegrant(s), lubricant(s), plasticizer(s), colorant(s), or carrier vehicle(s).
- Preferred unit forms are liquids or aerosols.
- compositions will include activity effective amounts of the active agent or can include less than such an amount if multiple applications are to be used to deliver or apply a total activity effective amount of the active agent.
- Unit forms are prepared by methods conventional in the art.
- the compositions of the subject invention are useful for applying non-biologically active agents to any substrates such as for example, skin, air, fixtures, carpets, hard or soft surfaces, water systems, etc., particularly in those in which pH changes.
- the compositions are applied by, for example, contacting the composition with the substrate.
- Clove oil/proteinoid microspheres were prepared by combining a mixture of 0.1 % clove oil in 10% soluble proteinoid (Glu-Asp-Tyr-Phe) with an equal volume of 1.7 N citric acid and gum to prepare a microsphere of clove oil/proteinoid microsphere.
- Clove oil is a relatively simple mixture of products obtained from the extraction of clove.
- the major components in the extract are a mixture of eugenol, caryophyllene, eugenol acetate, humulene, and copaene. Their structures are shown below.
- a 1 mL aliquot of the suspension was transferred to three separate 20 mL crimp-top vials. Two of the three vials were adjusted to pH 4 and 7, respectively, by addition of several drops of aqueous NaOH. All the vials were th en sealed and placed in a Tekmar 7000 head-space autosampler.
- a 0.05% clove oil in 0.85 N citric acid and gum mixture was prepared as a control, and 1 mL was transferred to a separate head-space vial.
- An additional sample was prepared by transferring the bulk amorphous material obtained from the preparation of the proteinoid to a vial containing 1 mL of 0.85 N citric acid and gum.
- Each vial was individually heated for 5 minutes at 80°C, and then was pressurized to 300 psi for 3 minutes with helium.
- the head-space of each vial was equilibrated in a 250 ⁇ L injection loop and then transferred to an HP 5890 gc equipped with an HP 5971 MSD.
- the components in the vial head- space were chromatographed on an HP cross-linked 5% methylphenyl silicon column.
- the composition of the separate peaks in the chromatograms were identified by comparing their mass spectrum with the mass spectrum of authentic samples from a Wiley database.
- injector port temperature 250°C
- Oven temperature 100°C for 2 minutes
- detector transfer line temperature 180°C.
- Table 3 and Figure 2 illustrate that the relative composition of the head- space from the bulk amorphous sample closely parallels that from the control, but that there are subtle variations in the relative composition of the head-space of the clove/oil microspheres with increasing pH.
- the concentration of eugenol relative to the total clove-oil composition decreases. This suggests that the eugenol may be free or weakly bound to the microspheres.
- the hydrophobic components in the clove oil i.e. caryophyllene, humulene, and copaene, are released from the microspheres upon dissolution. Therefore, eugenol makes a smaller contribution to the total head-space composition of the clove-oil microspheres.
- Example 1 The method of Example 1 is followed substituting modified hydrolyzed soybean protein for the proteinoid.
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96921566A EP0831784A4 (en) | 1995-06-07 | 1996-06-06 | Fragrances and flavorants |
AU62765/96A AU6276596A (en) | 1995-06-07 | 1996-06-06 | Fragrances and flavorants |
JP9502232A JPH11507916A (en) | 1995-06-07 | 1996-06-06 | Air fresheners and fragrances |
MX9709085A MX9709085A (en) | 1996-06-06 | 1996-06-06 | Fragrances and flavorants. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/484,293 US5824345A (en) | 1995-06-07 | 1995-06-07 | Fragrances and flavorants |
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EP (1) | EP0831784A4 (en) |
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- 1995-06-07 US US08/484,293 patent/US5824345A/en not_active Expired - Lifetime
-
1996
- 1996-06-06 CA CA002219035A patent/CA2219035A1/en not_active Abandoned
- 1996-06-06 EP EP96921566A patent/EP0831784A4/en not_active Withdrawn
- 1996-06-06 JP JP9502232A patent/JPH11507916A/en active Pending
- 1996-06-06 WO PCT/US1996/010183 patent/WO1996040070A1/en not_active Application Discontinuation
- 1996-06-06 AU AU62765/96A patent/AU6276596A/en not_active Abandoned
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See also references of EP0831784A4 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2002518306A (en) * | 1998-06-18 | 2002-06-25 | ジエイ クロンク ピーター | Adhesive armored nasal strips and dilators containing drugs and fragrances |
JP2011101809A (en) * | 1998-06-18 | 2011-05-26 | J Cronk Peter | Nasal dilator |
JP4768123B2 (en) * | 1998-06-18 | 2011-09-07 | ジエイ クロンク ピーター | Usage of nasal dilators and nasal dilation strips |
WO2005112937A1 (en) | 2004-05-19 | 2005-12-01 | Emisphere Technologies, Inc. | Acyclovir formulations |
WO2006072070A2 (en) | 2004-12-29 | 2006-07-06 | Emisphere Technologies, Inc. | Pharmaceutical formulations of gallium salts |
US8110547B2 (en) | 2005-01-12 | 2012-02-07 | Emisphere Technologies, Inc. | Compositions for buccal delivery of parathyroid hormone |
US8927015B2 (en) | 2006-04-12 | 2015-01-06 | Emisphere Technologies, Inc. | Formulations for delivering insulin |
US9364502B2 (en) | 2006-06-28 | 2016-06-14 | Emisphere Technologies, Inc. | Gallium nitrate formulations |
US8834514B2 (en) | 2006-08-30 | 2014-09-16 | Xennovate Medical Llc | Resilient band medical device |
WO2011017346A2 (en) | 2009-08-03 | 2011-02-10 | Emisphere Technologies, Inc. | Fast-acting naproxen composition with reduced gastrointestinal effects |
Also Published As
Publication number | Publication date |
---|---|
AU6276596A (en) | 1996-12-30 |
EP0831784A1 (en) | 1998-04-01 |
EP0831784A4 (en) | 2000-07-19 |
JPH11507916A (en) | 1999-07-13 |
CA2219035A1 (en) | 1996-12-19 |
US5824345A (en) | 1998-10-20 |
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