WO1997034908A1 - Verfahren und vorrichtung zur isolierung von nukleinsäuren - Google Patents
Verfahren und vorrichtung zur isolierung von nukleinsäuren Download PDFInfo
- Publication number
- WO1997034908A1 WO1997034908A1 PCT/DE1997/000517 DE9700517W WO9734908A1 WO 1997034908 A1 WO1997034908 A1 WO 1997034908A1 DE 9700517 W DE9700517 W DE 9700517W WO 9734908 A1 WO9734908 A1 WO 9734908A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acids
- elution
- electrophoresis
- electrodes
- reaction space
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
- G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom
Definitions
- the invention relates to a method and a device for isolating nucleic acids.
- PCR polymerase chain reaction
- nucleic acids e.g. hybridization, purification and concentration of the nucleic acids to be analyzed play an important role.
- step a the liquid sample is passed through a glass fiber fleece on which the nucleic acids adsorb. Then the glass fiber fleece is included different solutions washed. Finally, the nucleic acids are eluted from the solid phase in the presence of low ionic strength buffers.
- the known method is disadvantageous in several respects: When the solid phase is washed, the sample can become contaminated. In addition, due to the capillary forces in the glass fiber fleece, the nucleic acids can only be partially recovered from it.
- nucleic acids are bound to gels and then brought back into solution by means of electroelution. This can also contaminate the solution.
- the nucleic acids are present in the solution in high dilution. There is no concentration.
- the object of the present invention is to provide a method and a device with which the disadvantages of the prior art are avoided.
- a method and a device for isolating nucleic acids are to be specified which enable simple and inexpensive purification and concentration of nucleic acids.
- the invention aims to avoid contamination.
- SPARE BLADE (RULE 26) According to the invention, a method for isolating nucleic acids from biological liquids and suspensions containing nucleic acids is provided, wherein
- nucleic acids are bound to an adsorbent
- the method enables a simple way to purify and concentrate nucleic acids from liquids.
- the risk of contamination can largely be ruled out, particularly in the case of an automatic process control.
- Elution can be done by changing the buffer or electrically by electroelution or electrophoresis.
- a device for isolating nucleic acids from biological liquids and suspensions containing nucleic acids is provided, a reaction space for receiving an adsorbent loaded with nucleic acids being connected to a removal space and the nucleic acids being movable from the reaction space into the removal space by means of an electrophoresis device and can be enriched there.
- This device enables a simple and inexpensive concentration and isolation of nucleic acids. By providing a special one Contamination can largely be excluded in the removal room.
- FIG. 1 is a schematic cross-sectional view of a first exemplary embodiment of the device
- FIG. 2 shows the embodiment of FIG. 1 with "spin column"
- FIG. 3 shows a schematic cross-sectional view of a second exemplary embodiment of the device
- FIG. 4 shows a schematic cross-sectional view through a first exemplary embodiment of a purification and enrichment device with a device according to FIG. 3,
- FIG. 5 is a plan view of an agarose flat bed gel
- 6a is a schematic cross-sectional view of a third embodiment of the device.
- FIG. 6b shows a modification of the embodiment shown in FIG. 6a
- 6c shows a schematic cross-sectional view of a fourth exemplary embodiment of the device
- FIG. 7a is a bottom view of a fifth embodiment of the device.
- ERSATZBL ⁇ TT (REGEL26) 7b is a plan view of the embodiment of FIG. 7a
- FIG. 7c is a schematic side view of the embodiment of FIG. 7a
- Fig. 7d is a perspective view of a cover for a first embodiment of a
- FIG. 7e is a perspective view of the first embodiment of the elution device without a lid
- FIGS. 7a to 7c are perspective views of the embodiment of FIGS. 7a to 7c.
- FIG. 8 is a schematic cross-sectional view through a sixth embodiment of the device.
- FIG. 10 shows a schematic cross-sectional view through a seventh exemplary embodiment of the device
- FIG. 11 is a schematic cross-sectional view through an eighth embodiment of the device.
- Fig. 12 is a plan view of a second embodiment of an elution device.
- FIG. 13 shows a schematic cross-sectional view through a second exemplary embodiment of a purification and enrichment device.
- REPLACEMENT BUTT (RULE 26) 1 shows a schematic cross section of a first exemplary embodiment of the device.
- a container 15 is accommodated in an electrophoresis buffer tank 10 with a bottom opening 43.
- the container 15 encloses a reaction space 17, which is connected to a removal space 50 via a channel 49.
- the volume of the reaction space 17 is preferably 1 to 20 ml.
- the removal space 50 is ionically connected to an electrophoresis buffer accommodated in the electrophoresis buffer tank 10 by means of a first permeable membrane 30 closing a first opening 42.
- the volume of the removal space 50 is preferably 0.005 to 0.1 ml.
- the first permeable membrane 30 is formed, for example, from a dialysis membrane which is not permeable to nucleic acids, but is permeable to salts, in particular chaotropic salts.
- the first permeable membrane 30 is by means of a flexible ring, e.g. a 0-ring, attached to a first nozzle 41.
- An adsorbent 100 e.g. a glass fiber fleece, silica, glass beads, glass-containing magnetic particles, anion exchangers or the like, added.
- a cathode 20a projects into the reaction space 17 through a second opening 80.
- An anode 20b dips into the electrophoresis buffer located in the electrophoresis buffer tank 10, the fill level of which is designated by 70.
- a second nozzle 85 extends from the container 15 and passes through the opening 43.
- the second nozzle 85 is sealed off from the electrophoresis buffer tank 10 by means of an O-ring 40.
- the removal space 50 has a removal opening 60. It is designed so that air pockets are avoided.
- the removal space 50 can in particular be designed as a capillary.
- FIG. 2 essentially shows the first exemplary embodiment shown in FIG. 1.
- a spin column "90 is included.
- a second nozzle 85 passing through the electrophoresis buffer tank 10 is not provided here.
- FIG. 3 shows a schematic cross-sectional view through a second exemplary embodiment of the device.
- the cathode 20a is located outside the reaction space 17. It is immersed directly in the electrophoresis buffer tank 10.
- a third nozzle 45 is provided, the third opening 46 of which is closed by a second permeable membrane 31.
- FIG. 4 shows a schematic cross-sectional view through a first exemplary embodiment of a purification and enrichment device with the device according to FIG. 3.
- the device according to FIG. 3 is in the effective range of an x, y, z pipettor whose x, y, z Pipetting arm is designated 190.
- the x, y, z pipette arm 190 receives a pipette tip 180, preferably a disposable tip.
- a suitable x, y, z pipettor is, for example. offered by TECAN AG, Switzerland.
- a heatable shaking block 170 is arranged in the effective area of the x, y, z pipettor.
- Reaction tubes 150 for lysis are accommodated in the debris block 170.
- a first vessel 160 for lysis a second vessel 162 for receiving a wash solution and sample vessels 165.
- a supply of pipette tips is designated by 185 and PCR vessels are designated by 210.
- the electrophoresis buffer tank 10 is provided with a full nozzle 125 which is connected to an electrophoresis buffer supply 110 with the interposition of a pump 120.
- the second connection piece 85 and a discharge line 140 provided at the bottom of the electrophoresis buffer tank 10 are connected to a second pump 130 m.
- the second pump 130 is preferably designed as a hose pump.
- a suitable hose pump is e.g. offered by Cavro, California, USA.
- the cathode 20a and the anode 20b are connected to a voltage source 220 via electrical leads 225a and 225b.
- the first 120 and the second pump 130, the shaking device 170, the x, y, z pipettor and the voltage source 220 are designed so that they can be controlled by means of a process computer. A fully automatic operation of the purification and enrichment device is thus possible.
- FIG. 5 shows a top view of a particularly simple variant of a container 15. This is made from an agarose flat bed gel 11, on the opposite transverse sides of which the cathode 20a and the anode 20b rest.
- a first recess 81 forming the reaction space 17 for receiving adsorbent
- a second recess 61 forming the reaction space 17 for receiving adsorbent
- the second recess 61 is slit-shaped. It serves to remove the nucleic acids enriched therein.
- FIG. 6a shows a schematic cross-sectional view through a third exemplary embodiment of the device.
- the container 15 is designed in the form of a cross connector.
- the adsorbent 100 is located on a support fleece 302.
- the cathode 20a and the anode 20b are formed as electrically conductive plastic pipette tips, which are connected to the electrophoresis buffer tank (not shown here). They are connected to the container 15 via hose pieces 318. Nucleic acids enriched in the removal space 50 can be withdrawn through the removal opening 60.
- the container 15 consists of a cuboid part.
- the reaction space 17 is formed by a hole.
- Recesses 320, 321 for receiving the cathode 20a and the anode 20b are provided on both sides next to the reaction space 17.
- the wall between the recesses 320, 321 and the reaction space 17 is ion-conductive.
- the recesses 320 serve to receive electrophoresis buffers. They are closed with a plug 340.
- the electrophoresis buffer tank consists of two partial containers which surround the reaction space 17. A plurality of such devices, of which another is shown in perspective in FIG.
- the elution device can be components of the first exemplary embodiment of an elution device shown in FIGS. 7d and 7e.
- the elution device essentially consists of a multiple container 410 for holding several devices according to FIG. 7f.
- the multiple container 410 has a vacuum connection 401 and connections 402 for a liquid circuit for heating the elution device.
- the lid 400 shown in FIG. 7d is provided with leads 226a, 226b for the electrodes.
- the cathode 20a is integrated in the wall of the reaction space 17 and the anode 20b m the opposite wall of the removal space 50.
- Eme permeable, in particular a semipermeable, membrane 310 is provided in the removal space 50, which membrane is impermeable to nucleic acids. The membrane 310 prevents the nucleic acids from reaching the anode 20b and
- SPARE BLADE (RULE 26) be destroyed there by redox processes.
- the adsorbent 100 is supported by a support fleece 302 at the entrance of the second nozzle 85.
- a first layer 323 applied to the noble metal or plastic consists of biotinylated bovine serum albumin, a second layer 325 resting thereon consists of a streptavidin or polystreptavidin and an outer third layer 324 is formed from an oligonucleotide.
- a movable permanent magnet 312 is arranged on the outside of the container 15 in such a way that its north pole is in the vicinity of the cathode 20a.
- the reaction space 17 is closed with a transparent snap cover 316.
- the removal space 50 is closed with a snap cover 326, which is provided with a septum 328.
- the septum 328 can be pierced by means of a needle 327 to remove or add liquid. Contamination of the liquid in the device can thus be avoided.
- 314 denotes a photomultiplier, which is arranged above the transparent snap cover 316.
- FIG. 11 shows a schematic cross section of an eighth exemplary embodiment.
- the movable permanent magnet 312 with its south pole is arranged near the outside of the anode 20b.
- the bottom of the removal space 50 is transparent. Is located opposite the removal opening 60
- FIG. 12 shows a plan view of a second exemplary embodiment of an elution device.
- a plurality of the devices shown in FIG. 11 are arranged side by side.
- Thermostatic plates 329 are provided on each of the long sides of the devices, with which the temperature can be adjusted.
- FIG. 13 shows a schematic cross-sectional view of a second exemplary embodiment of a purification and enrichment device.
- the electrodes 20a and 20b of a device according to FIG. 11 are connected to a voltage source 220.
- the device according to FIG. 11 is located in the effective range of an x, y, z pipetting arm 190 of an x, y, z pipetting robot.
- the voltage source 220, the second pump 130 for the disposal of solutions to be discarded, an apparatus (not shown here) for moving a permanent magnet 312 and the x, y, z pipetting robot can be controlled by means of a process computer, e.g. a personal computer, fully automatically controllable.
- Biological liquids containing nucleic acid to be analyzed are brought into contact with an adsorbent 100 m.
- the nucleic acids present in the solution adsorb onto the adsorbent 100.
- the adsorbent 100 loaded with the nucleic acids, for example. em Spm-column 90, the reaction space 17 is inserted through the second opening 80 m.
- REPLACEMENT SHEET (RULE 26) Charged nucleic acids are thereby released from the adsorbent 100 and moved in the direction of the anode 20b arranged in the vicinity of the removal space 50.
- a first permeable membrane 30 which is impermeable to nucleic acids is provided.
- the current passed through the electrophoresis buffer is turned off.
- An elution volume containing enriched nucleic acids can now be removed through the removal opening 60 of the removal space 50.
- the removal opening 60 can be closed with the snap lid 326, which is provided with a septum 328.
- the septum 328 can be pierced with a needle 327 to remove elution volume.
- electrodes 20a, 20b of different designs can be used.
- the device according to the invention can be combined with a device for the detection of chemiluminescence.
- a photomultiplier 314 is arranged in the vicinity of the container 15.
- the nucleic acids are moved electrophoretically by the adsorbent 100 m in the direction of the anode 20b.
- an amplification of the nucleic acids can be carried out.
- the amplified nucleic acids are then bound by adding magnetic particles.
- the permanent magnet 312 By moving the permanent magnet 312 to the anode 20b, the nucleic acids are loaded
- ERS ⁇ ZBL ⁇ TT (REGEL26) Magnetic particles attracted to the anode 20b. After adding a chemiluminescent buffer and applying a voltage across the electrodes 20a, 20b, a chemiluminescent is triggered. The light emitted in the process is detected by the photomultiplier 314.
- the above-mentioned functions can be automated by means of an x, y, z pipetting robot. It is thus possible to automatically operate a plurality of the devices according to the invention one after the other.
- the first recess 81 serves to receive the glass fleece and the second recess 61 is filled with electrophoresis buffer. In this way, the nucleic acid can be eluted electrophoretically from the glass fleece and transferred into the subsequent agarose gel and into the second recess 61.
- the isolated concentrated nucleic acid was removed from the second recess 61.
- SPARE BLADE (RULE 26) 20a, 20b, wires with a diameter of 0.3 mm of a platinum-ruthenium alloy were used and as a voltage generator of the electrophoresis voltage generator from Hölzel, Dorfen. 30 ⁇ l of elution volume with the nucleic acid were removed from the removal opening 60.
- EDTA whole blood was collected from the carotid artery from a freshly slaughtered white broiler and immediately mixed with ethylenediaminetetraacetic acid (Sigma, Kunststoff, Order No. E-5513) in a concentration of 0.06 g EDTA / ml whole blood.
- the EDTA whole blood was frozen in portions and stored at -15 ° C. All reagents are taken from the "High Pure PCR Template Preparation Kit" from Boehringer Mannheim (order no. 1 796 828). 100 ul EDTA whole blood (see above) were mixed with 200 ul lysis buffer and 60 ul proteinase K from the above Reagent set mixed and 15 min. incubated at 70 ° C.
- SPARE BLADE by applying a DC voltage of max. 10 mA at approx. 60 degrees. C performed.
- Biotinylated bovine immunoglobulin G (R-IgG) is first produced. 0.5 ml of an R-IgG solution (2 mg R-IgG
- the disks are then washed 3 times with 100 ml of Milli-Q water, the solid and liquid phases being separated by sedimentation or centrifugation.
- the slices are then taken up again in 40 ml of PBS.
- the device shown in FIG. 14 was automatically controlled with a computer program with the following program steps for carrying out the isolation, amplification and chemiluminescence measurement:
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/142,958 US6071395A (en) | 1996-03-15 | 1997-03-14 | Process and device for isolating nucleic acids |
JP53303497A JP2001503730A (ja) | 1996-03-15 | 1997-03-14 | 核酸を分離するための方法および装置 |
DE59704304T DE59704304D1 (de) | 1996-03-15 | 1997-03-14 | Verfahren und vorrichtung zur isolierung von nukleinsäuren |
EP97920510A EP0891369B1 (de) | 1996-03-15 | 1997-03-14 | Verfahren und vorrichtung zur isolierung von nukleinsäuren |
AT97920510T ATE204285T1 (de) | 1996-03-15 | 1997-03-14 | Verfahren und vorrichtung zur isolierung von nukleinsäuren |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19610354A DE19610354C1 (de) | 1996-03-15 | 1996-03-15 | Vorrichtung, Verfahren und Einrichtung zur Isolierung von Nukleinsäuren |
DE19610354.1 | 1996-03-15 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/384,936 Division US6232464B1 (en) | 1996-03-15 | 1999-08-27 | Process and apparatus for isolating nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997034908A1 true WO1997034908A1 (de) | 1997-09-25 |
Family
ID=7788478
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE1997/000517 WO1997034908A1 (de) | 1996-03-15 | 1997-03-14 | Verfahren und vorrichtung zur isolierung von nukleinsäuren |
Country Status (6)
Country | Link |
---|---|
US (2) | US6071395A (de) |
EP (1) | EP0891369B1 (de) |
JP (1) | JP2001503730A (de) |
AT (1) | ATE204285T1 (de) |
DE (2) | DE19610354C1 (de) |
WO (1) | WO1997034908A1 (de) |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0933132A2 (de) * | 1998-02-02 | 1999-08-04 | Toyo Boseki Kabushiki Kaisha | Gerät zur Extraktion von Nukleinsäuren |
FR2777293A1 (fr) * | 1998-04-10 | 1999-10-15 | Bio Merieux | Procede d'electro-separation d'un echantillon biologique et dispositif de mise en oeuvre |
US6071395A (en) * | 1996-03-15 | 2000-06-06 | Lange; Hans | Process and device for isolating nucleic acids |
WO2000071999A1 (de) * | 1999-05-19 | 2000-11-30 | Bilatec Ag | Vorrichtung und verfahren zur isolierung von elektrisch geladenen molekülen |
WO2001007452A1 (en) * | 1999-07-27 | 2001-02-01 | Dna Research Innovations Limited | Extraction of nucleic acids |
US6264814B1 (en) | 1997-06-14 | 2001-07-24 | Bilatec Gesellschaft Zur Entwicklung | Apparatus and method for isolating and/or analyzing charged molecules |
JP2002513936A (ja) * | 1998-05-01 | 2002-05-14 | ジェン−プロウブ インコーポレイテッド | 自動化診断用分析器および方法 |
JP2009254384A (ja) * | 1997-10-31 | 2009-11-05 | Bbi Bioseq Inc | 圧力強化抽出及び精製方法 |
US7794659B2 (en) | 2005-03-10 | 2010-09-14 | Gen-Probe Incorporated | Signal measuring system having a movable signal measuring device |
US8192992B2 (en) | 1998-05-01 | 2012-06-05 | Gen-Probe Incorporated | System and method for incubating the contents of a reaction receptacle |
US8718948B2 (en) | 2011-02-24 | 2014-05-06 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
US8840848B2 (en) | 2010-07-23 | 2014-09-23 | Beckman Coulter, Inc. | System and method including analytical units |
US8973736B2 (en) | 2011-11-07 | 2015-03-10 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
US9046506B2 (en) | 2011-11-07 | 2015-06-02 | Beckman Coulter, Inc. | Specimen container detection |
US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
US9446418B2 (en) | 2011-11-07 | 2016-09-20 | Beckman Coulter, Inc. | Robotic arm |
US9482684B2 (en) | 2011-11-07 | 2016-11-01 | Beckman Coulter, Inc. | Centrifuge system and workflow |
US9506943B2 (en) | 2011-11-07 | 2016-11-29 | Beckman Coulter, Inc. | Aliquotter system and workflow |
WO2016193282A1 (en) | 2015-06-01 | 2016-12-08 | Qiagen Gmbh | Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach |
WO2016193281A1 (en) | 2015-06-01 | 2016-12-08 | Qiagen Gmbh | Electrophoresis assisted method and device for purifying a charged target molecule from a sample |
US9910054B2 (en) | 2011-11-07 | 2018-03-06 | Beckman Coulter, Inc. | System and method for processing samples |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
EP0953842A1 (de) * | 1998-05-01 | 1999-11-03 | F. Hoffmann-La Roche Ag | Analysenautomat mit an der Unterseite verjüngter Mischkammer und mit dieser dichtend verbundener Sockeleinheit |
DE60030310T2 (de) * | 1999-10-20 | 2007-08-23 | Gentra Systems Inc., Minneapolis | Mix- und giessapparat mit drehbarem arm und zugehörigem gefäss |
DE10006214A1 (de) * | 2000-02-11 | 2001-08-16 | Roche Diagnostics Gmbh | System zur einfachen Nukleinsäureanalytik |
US7842246B2 (en) | 2001-06-29 | 2010-11-30 | Meso Scale Technologies, Llc | Assay plates, reader systems and methods for luminescence test measurements |
EP1552005A4 (de) * | 2002-07-10 | 2010-12-15 | Massachusetts Inst Technology | Vorrichtung und verfahren zur isolierung einer nukleinsäure aus einer probe |
GB0319671D0 (en) * | 2003-08-21 | 2003-09-24 | Secr Defence | Apparatus for processing a fluid sample |
JP2005110503A (ja) * | 2003-10-02 | 2005-04-28 | Arkray Inc | 核酸の精製方法及びデバイス |
US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
FR2867695B1 (fr) * | 2004-03-18 | 2006-12-29 | Portmann Instr | Dispositif et procede de filtration et/ou de separation de molecules |
DE102004058828B4 (de) * | 2004-10-28 | 2010-08-19 | Progen Biotechnik Gmbh | Vorrichtung und Verfahren zur parallelen Aufbereitung von Biopolymeren |
CN101370622B (zh) | 2006-01-18 | 2013-07-31 | 阿戈斯治疗公司 | 用于处理封闭容器中的样品的系统和方法以及相关装置 |
WO2008085991A2 (en) * | 2007-01-08 | 2008-07-17 | U.S. Genomics, Inc. | Reaction chamber |
US8703492B2 (en) * | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
US8357538B2 (en) * | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
US20100294665A1 (en) * | 2007-07-12 | 2010-11-25 | Richard Allen | Method and system for transferring and/or concentrating a sample |
US8361716B2 (en) * | 2008-10-03 | 2013-01-29 | Pathogenetix, Inc. | Focusing chamber |
US9953141B2 (en) | 2009-11-18 | 2018-04-24 | Becton, Dickinson And Company | Laboratory central control unit method and system |
EP2661485A4 (de) | 2011-01-06 | 2018-11-21 | Meso Scale Technologies, LLC | Testkassetten und verfahren zu ihrer verwendung |
US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
JP6165961B2 (ja) | 2013-03-15 | 2017-07-19 | アボット・ラボラトリーズAbbott Laboratories | 前処理カルーセルを有する診断分析装置および関連方法 |
ES2901756T3 (es) | 2013-03-15 | 2022-03-23 | Abbott Lab | Analizadores de diagnóstico automatizados que tienen sistemas de pista accesibles por la parte trasera y métodos relacionados |
US9400285B2 (en) | 2013-03-15 | 2016-07-26 | Abbot Laboratories | Automated diagnostic analyzers having vertically arranged carousels and related methods |
US9766206B2 (en) | 2013-09-27 | 2017-09-19 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
DE102015213417A1 (de) * | 2015-07-16 | 2017-01-19 | Dennert Poraver Gmbh | Verfahren und Anlage zur Behandlung von ammoniumhaltigem Abwasser |
CN108130273B (zh) * | 2016-12-01 | 2021-10-12 | 京东方科技集团股份有限公司 | 检测基板及其制作方法、检测核酸的方法 |
US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
WO2019014306A2 (en) * | 2017-07-13 | 2019-01-17 | Argos Therapeutics, Inc. | SYSTEMS, APPARATUS, METHODS AND ASSOCIATED DEVICES FOR AUTOMATED PROCESSING |
KR102001137B1 (ko) * | 2017-07-28 | 2019-07-17 | (주)옵토레인 | 시료채취장치, 그 제조방법, 및 이를 이용한 시료채취방법 |
WO2019032385A1 (en) * | 2017-08-09 | 2019-02-14 | Helena Laboratories Corporation | ELECTROPHORESIS APPARATUS WITH PLANAR ELECTRODE CONTACT SURFACES |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2402716A1 (fr) * | 1977-09-07 | 1979-04-06 | Inst Nat Sante Rech Med | Procede et dispositif pour la purification de macromolecules chargees, notamment de macromoleculres biologiques et macromolecules ainsi traitees |
DE4139664A1 (de) * | 1991-12-02 | 1993-06-03 | Diagen Inst Molekularbio | Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren |
US5340449A (en) * | 1990-12-07 | 1994-08-23 | Shukla Ashok K | Apparatus for electroelution |
EP0687502A2 (de) * | 1994-06-15 | 1995-12-20 | Roche Diagnostics GmbH | Vorrichtung zur Behandlung von Nukelinsäuren aus einer Probe |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE281354C (de) * | ||||
GB1339004A (en) * | 1970-05-19 | 1973-11-28 | Pye Ltd | Chromatographic apparatus |
US3969218A (en) * | 1975-02-11 | 1976-07-13 | The United States Of America As Represented By The United States Energy Research And Development Administration | Elution electrophoresis |
US4164464A (en) * | 1977-03-25 | 1979-08-14 | Instrumentation Specialties Company | Sample concentrator |
US4222836A (en) * | 1979-06-01 | 1980-09-16 | Corning Glass Works | Acidic agar gel electrochromatography of glycohemoglobins |
US4413505A (en) * | 1981-03-09 | 1983-11-08 | Environmental Sciences Associates, Inc. | Electrochemical flow cell, particularly use with liquid chromatography |
US4459198A (en) * | 1981-07-27 | 1984-07-10 | Shimadzu Corporation | Electrophoretic apparatus |
US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
FR2574311B1 (fr) * | 1984-12-11 | 1989-09-15 | Inst Nat Sante Rech Med | Perfectionnements aux procedes et appareils d'electrophorese preparative |
US4861555A (en) * | 1985-03-11 | 1989-08-29 | Applied Automation, Inc. | Apparatus for chromatographic analysis of ionic species |
US4695555A (en) * | 1985-06-12 | 1987-09-22 | Keeffe Andrew F O | Liquid chromatographic detector and method |
US4699706A (en) * | 1986-05-30 | 1987-10-13 | Bio-Rad Laboratories, Inc. | Method and apparatus for extraction of species from electrophoresis media |
US4708782A (en) * | 1986-09-15 | 1987-11-24 | Sepragen Corporation | Chromatography column-electrophoresis system |
DD281354A5 (de) * | 1987-08-11 | 1990-08-08 | Univ Berlin Humboldt | Flaechentraeger zur adsorption, immobilisierung, flaechenbettchromatographie und elektrophorese von nukleinsaeuren und nukleoproteinen |
EP0446260B1 (de) * | 1988-11-21 | 1994-03-02 | Dynal A.S. | Sonden aus nukleinsäuren |
DE3913814A1 (de) * | 1989-01-13 | 1990-07-19 | Max Planck Gesellschaft | Verfahren zur elektrischen elution von auf trenngelen gebundenen, elektrisch geladenen substanzen und eine anordnung zur durchfuehrung des verfahrens |
US4964961A (en) * | 1989-08-02 | 1990-10-23 | E-C Apparatus Corporation | Elution method and device |
US5340452A (en) * | 1991-02-01 | 1994-08-23 | Beckman Instruments, Inc. | On-column preconcentration of samples in capillary electrophoresis |
US5453382A (en) * | 1991-08-05 | 1995-09-26 | Indiana University Foundation | Electrochromatographic preconcentration method |
CA2087481C (en) * | 1992-02-10 | 2001-09-04 | John R. Stillian | Ion chromatography system using electrochemical suppression and detector effluent recycle |
US5830642A (en) * | 1992-04-03 | 1998-11-03 | Amersham Life Science, Inc. | Electrophoresis of nucleic acid fragments |
DE4412286A1 (de) * | 1994-04-09 | 1995-10-12 | Boehringer Mannheim Gmbh | System zur kontaminationsfreien Bearbeitung von Reaktionsabläufen |
US6045995A (en) * | 1994-08-24 | 2000-04-04 | Isis Pharmaceuticals, Inc. | Capillary electrophoretic detection of nucleic acids |
US5569365A (en) * | 1995-03-03 | 1996-10-29 | Dionex Corporation | Intermittent electrolytic membrane suppressor regeneration for ion chromatography |
JP2965131B2 (ja) * | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
DE19610354C1 (de) * | 1996-03-15 | 1997-11-20 | Innova Gmbh | Vorrichtung, Verfahren und Einrichtung zur Isolierung von Nukleinsäuren |
DE19626234A1 (de) * | 1996-06-29 | 1998-01-02 | Innova Gmbh | Vorrichtung zur kontaminationsfreien Zu- und Abfuhr von Flüssigkeiten |
-
1996
- 1996-03-15 DE DE19610354A patent/DE19610354C1/de not_active Expired - Fee Related
-
1997
- 1997-03-14 JP JP53303497A patent/JP2001503730A/ja active Pending
- 1997-03-14 US US09/142,958 patent/US6071395A/en not_active Expired - Fee Related
- 1997-03-14 DE DE59704304T patent/DE59704304D1/de not_active Expired - Fee Related
- 1997-03-14 WO PCT/DE1997/000517 patent/WO1997034908A1/de active IP Right Grant
- 1997-03-14 AT AT97920510T patent/ATE204285T1/de not_active IP Right Cessation
- 1997-03-14 EP EP97920510A patent/EP0891369B1/de not_active Expired - Lifetime
-
1999
- 1999-08-27 US US09/384,936 patent/US6232464B1/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2402716A1 (fr) * | 1977-09-07 | 1979-04-06 | Inst Nat Sante Rech Med | Procede et dispositif pour la purification de macromolecules chargees, notamment de macromoleculres biologiques et macromolecules ainsi traitees |
US5340449A (en) * | 1990-12-07 | 1994-08-23 | Shukla Ashok K | Apparatus for electroelution |
DE4139664A1 (de) * | 1991-12-02 | 1993-06-03 | Diagen Inst Molekularbio | Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren |
EP0687502A2 (de) * | 1994-06-15 | 1995-12-20 | Roche Diagnostics GmbH | Vorrichtung zur Behandlung von Nukelinsäuren aus einer Probe |
Cited By (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6071395A (en) * | 1996-03-15 | 2000-06-06 | Lange; Hans | Process and device for isolating nucleic acids |
US6232464B1 (en) * | 1996-03-15 | 2001-05-15 | Hans Lange | Process and apparatus for isolating nucleic acids |
US6264814B1 (en) | 1997-06-14 | 2001-07-24 | Bilatec Gesellschaft Zur Entwicklung | Apparatus and method for isolating and/or analyzing charged molecules |
JP2009254384A (ja) * | 1997-10-31 | 2009-11-05 | Bbi Bioseq Inc | 圧力強化抽出及び精製方法 |
EP0933132A2 (de) * | 1998-02-02 | 1999-08-04 | Toyo Boseki Kabushiki Kaisha | Gerät zur Extraktion von Nukleinsäuren |
EP0933132A3 (de) * | 1998-02-02 | 2000-12-06 | Toyo Boseki Kabushiki Kaisha | Gerät zur Extraktion von Nukleinsäuren |
US6255478B1 (en) | 1998-02-02 | 2001-07-03 | Toyo Boseki Kabushiki Kaisha | Nucleic acid extraction method |
FR2777293A1 (fr) * | 1998-04-10 | 1999-10-15 | Bio Merieux | Procede d'electro-separation d'un echantillon biologique et dispositif de mise en oeuvre |
WO1999053304A1 (fr) * | 1998-04-10 | 1999-10-21 | Bio Merieux | Procede d'electro-separation d'un echantillon biologique et dispositif de mise en oeuvre |
US9598723B2 (en) | 1998-05-01 | 2017-03-21 | Gen-Probe Incorporated | Automated analyzer for performing a nucleic acid-based assay |
US8709814B2 (en) | 1998-05-01 | 2014-04-29 | Gen-Probe Incorporated | Method for incubating the contents of a receptacle |
US8569019B2 (en) | 1998-05-01 | 2013-10-29 | Gen-Probe Incorporated | Method for performing an assay with a nucleic acid present in a specimen |
US7666681B2 (en) | 1998-05-01 | 2010-02-23 | Gen-Probe Incorporated | Method for agitating the fluid contents of a container |
US7666602B2 (en) | 1998-05-01 | 2010-02-23 | Gen-Probe Incorporated | Method for agitating the fluid contents of a container |
US8546110B2 (en) | 1998-05-01 | 2013-10-01 | Gen-Probe Incorporated | Method for detecting the presence of a nucleic acid in a sample |
US8569020B2 (en) | 1998-05-01 | 2013-10-29 | Gen-Probe Incorporated | Method for simultaneously performing multiple amplification reactions |
US9150908B2 (en) | 1998-05-01 | 2015-10-06 | Gen-Probe Incorporated | Method for detecting the presence of a nucleic acid in a sample |
US8883455B2 (en) | 1998-05-01 | 2014-11-11 | Gen-Probe Incorporated | Method for detecting the presence of a nucleic acid in a sample |
JP2002513936A (ja) * | 1998-05-01 | 2002-05-14 | ジェン−プロウブ インコーポレイテッド | 自動化診断用分析器および方法 |
US8012419B2 (en) | 1998-05-01 | 2011-09-06 | Gen-Probe Incorporated | Temperature-controlled incubator having rotatable door |
US8137620B2 (en) | 1998-05-01 | 2012-03-20 | Gen-Probe Incorporated | Temperature-controlled incubator having an arcuate closure panel |
US8192992B2 (en) | 1998-05-01 | 2012-06-05 | Gen-Probe Incorporated | System and method for incubating the contents of a reaction receptacle |
US8221682B2 (en) | 1998-05-01 | 2012-07-17 | Gen-Probe Incorporated | System for incubating the contents of a reaction receptacle |
US8309358B2 (en) | 1998-05-01 | 2012-11-13 | Gen-Probe Incorporated | Method for introducing a fluid into a reaction receptacle contained within a temperature-controlled environment |
US8318500B2 (en) | 1998-05-01 | 2012-11-27 | Gen-Probe, Incorporated | Method for agitating the contents of a reaction receptacle within a temperature-controlled environment |
US8337753B2 (en) | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
WO2000071999A1 (de) * | 1999-05-19 | 2000-11-30 | Bilatec Ag | Vorrichtung und verfahren zur isolierung von elektrisch geladenen molekülen |
WO2001007452A1 (en) * | 1999-07-27 | 2001-02-01 | Dna Research Innovations Limited | Extraction of nucleic acids |
US7897337B2 (en) | 2005-03-10 | 2011-03-01 | Gen-Probe Incorporated | Method for performing multi-formatted assays |
US7932081B2 (en) | 2005-03-10 | 2011-04-26 | Gen-Probe Incorporated | Signal measuring system for conducting real-time amplification assays |
US8349564B2 (en) | 2005-03-10 | 2013-01-08 | Gen-Probe Incorporated | Method for continuous mode processing of the contents of multiple reaction receptacles in a real-time amplification assay |
US8663922B2 (en) | 2005-03-10 | 2014-03-04 | Gen-Probe Incorporated | Systems and methods for detecting multiple optical signals |
US8008066B2 (en) | 2005-03-10 | 2011-08-30 | Gen-Probe Incorporated | System for performing multi-formatted assays |
US10006862B2 (en) | 2005-03-10 | 2018-06-26 | Gen-Probe Incorporated | Continuous process for performing multiple nucleic acid amplification assays |
US9726607B2 (en) | 2005-03-10 | 2017-08-08 | Gen-Probe Incorporated | Systems and methods for detecting multiple optical signals |
US7964413B2 (en) | 2005-03-10 | 2011-06-21 | Gen-Probe Incorporated | Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay |
US7794659B2 (en) | 2005-03-10 | 2010-09-14 | Gen-Probe Incorporated | Signal measuring system having a movable signal measuring device |
US8501461B2 (en) | 2005-03-10 | 2013-08-06 | Gen-Probe Incorporated | System for performing multi-formatted assays |
US9372156B2 (en) | 2005-03-10 | 2016-06-21 | Gen-Probe Incorporated | System for processing contents of a receptacle to detect an optical signal emitted by the contents |
US8956570B2 (en) | 2010-07-23 | 2015-02-17 | Beckman Coulter, Inc. | System and method including analytical units |
US9274132B2 (en) | 2010-07-23 | 2016-03-01 | Beckman Coulter, Inc. | Assay cartridge with reaction well |
US8932541B2 (en) | 2010-07-23 | 2015-01-13 | Beckman Coulter, Inc. | Pipettor including compliant coupling |
US8840848B2 (en) | 2010-07-23 | 2014-09-23 | Beckman Coulter, Inc. | System and method including analytical units |
US9046455B2 (en) | 2010-07-23 | 2015-06-02 | Beckman Coulter, Inc. | System and method including multiple processing lanes executing processing protocols |
US9140715B2 (en) | 2010-07-23 | 2015-09-22 | Beckman Coulter, Inc. | System and method for controlling thermal cycler modules |
US8996320B2 (en) | 2010-07-23 | 2015-03-31 | Beckman Coulter, Inc. | System and method including analytical units |
US9519000B2 (en) | 2010-07-23 | 2016-12-13 | Beckman Coulter, Inc. | Reagent cartridge |
US9285382B2 (en) | 2010-07-23 | 2016-03-15 | Beckman Coulter, Inc. | Reaction vessel |
US8962308B2 (en) | 2010-07-23 | 2015-02-24 | Beckman Coulter, Inc. | System and method including thermal cycler modules |
US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
US8718948B2 (en) | 2011-02-24 | 2014-05-06 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
US9915613B2 (en) | 2011-02-24 | 2018-03-13 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
US10641707B2 (en) | 2011-02-24 | 2020-05-05 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
US8973736B2 (en) | 2011-11-07 | 2015-03-10 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
US9506943B2 (en) | 2011-11-07 | 2016-11-29 | Beckman Coulter, Inc. | Aliquotter system and workflow |
US9910054B2 (en) | 2011-11-07 | 2018-03-06 | Beckman Coulter, Inc. | System and method for processing samples |
US9482684B2 (en) | 2011-11-07 | 2016-11-01 | Beckman Coulter, Inc. | Centrifuge system and workflow |
US9446418B2 (en) | 2011-11-07 | 2016-09-20 | Beckman Coulter, Inc. | Robotic arm |
US10048284B2 (en) | 2011-11-07 | 2018-08-14 | Beckman Coulter, Inc. | Sample container cap with centrifugation status indicator device |
US10274505B2 (en) | 2011-11-07 | 2019-04-30 | Beckman Coulter, Inc. | Robotic arm |
US9046506B2 (en) | 2011-11-07 | 2015-06-02 | Beckman Coulter, Inc. | Specimen container detection |
WO2016193281A1 (en) | 2015-06-01 | 2016-12-08 | Qiagen Gmbh | Electrophoresis assisted method and device for purifying a charged target molecule from a sample |
WO2016193282A1 (en) | 2015-06-01 | 2016-12-08 | Qiagen Gmbh | Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach |
Also Published As
Publication number | Publication date |
---|---|
US6232464B1 (en) | 2001-05-15 |
US6071395A (en) | 2000-06-06 |
DE19610354C1 (de) | 1997-11-20 |
ATE204285T1 (de) | 2001-09-15 |
EP0891369B1 (de) | 2001-08-16 |
EP0891369A1 (de) | 1999-01-20 |
DE59704304D1 (de) | 2001-09-20 |
JP2001503730A (ja) | 2001-03-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO1997034908A1 (de) | Verfahren und vorrichtung zur isolierung von nukleinsäuren | |
EP0988536A1 (de) | Vorrichtung und verfahren zur isolierung oder/und analyse geladener biomoleküle | |
DE69737071T2 (de) | Integrierte mikrofluidische vorrichtungen | |
DE60010058T2 (de) | Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen | |
AT414209B (de) | Sammelgefäss für flüssigkeiten | |
DE69634794T2 (de) | Verfahren und gerät für die flüssigkeitsbehandlung mittels eines verteilers | |
US5102518A (en) | Electrophoresis apparatus and method for electroeluting desired molecules for further processing | |
EP2084280B1 (de) | Integriertes mikrofluidisches bauteil zum aufreinigen von analytmolekülen sowie verfahren zum aufreinigen | |
DE102017204267B4 (de) | Verfahren zur anreicherung von zellen aus einer probe und der nachfolgenden nukleinsäureisolierung aus diesen zellen | |
WO1988009201A1 (en) | Process and device for separating and cleaning molecules | |
EP0995096B1 (de) | Magnetstift zur konzentrierung und separierung von partikeln | |
WO2007051859A1 (de) | Vorrichtung und verfahren zur automatisierten isolierung und aufreinigung von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien | |
WO2000024927A1 (de) | Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen | |
DE10112505C1 (de) | Sensoranordnung und Vorrichtung zur amperometrischen und/oder potentiometrischen, pharmakologischen Wirkort- und/oder Wirkstofftestung sowie Verfahren zur amperometrischen und/oder potentiometrischen, pharmakologischen Wirkort- und/oder Wirkstofftestung | |
EP3816275A1 (de) | Aufreinigungsinstrument mit konzentrierten und angereicherten magnetischen beads | |
EP1644120A1 (de) | Vorrichtung und verfahren zum abtrennen von magnetischen oder magnetisierbaren partikeln aus einer flüssigkeit | |
EP1180238A1 (de) | Vorrichtung und verfahren zur isolierung von elektrisch geladenen molekülen | |
WO2019057345A1 (de) | Vorrichtung und verfahren zur immobilisierung von biomolekülen mit magnetischen partikeln | |
KR20140026400A (ko) | 핵산 전기 영동 방법, 핵산 농축 정제 방법, 핵산 전기 영동용 카트리지 및 핵산 전기 영동용 카트리지의 제조 방법 | |
EP0912470A1 (de) | Verfahren und vorrichtung zur aufreinigung und anreicherung von molekülen | |
KR20200110829A (ko) | 핵산 농축 및 추출 장치 | |
DE102007011866A1 (de) | Vorrichtung zur Aufnahme, Behandlung und Aufbewahrung kleinvolumiger Proben | |
DE19626234A1 (de) | Vorrichtung zur kontaminationsfreien Zu- und Abfuhr von Flüssigkeiten | |
US20190203364A1 (en) | Apparatus and method for electrochemical reduction of biochemical compositions for bioconjugation | |
DE10013513A1 (de) | Vorrichtung zum Transfer und Dosieren von fluiden Proben |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 1997920510 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 1997 533034 Kind code of ref document: A Format of ref document f/p: F |
|
WWP | Wipo information: published in national office |
Ref document number: 1997920510 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09142958 Country of ref document: US |
|
WWG | Wipo information: grant in national office |
Ref document number: 1997920510 Country of ref document: EP |