WO1999017117A1 - Analytical device with capillary reagent carrier - Google Patents
Analytical device with capillary reagent carrier Download PDFInfo
- Publication number
- WO1999017117A1 WO1999017117A1 PCT/US1998/020736 US9820736W WO9917117A1 WO 1999017117 A1 WO1999017117 A1 WO 1999017117A1 US 9820736 W US9820736 W US 9820736W WO 9917117 A1 WO9917117 A1 WO 9917117A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hollow fiber
- matrix
- capillary tube
- light
- analyte
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
Definitions
- the present invention relates to a test device and determination of a chemical or biochemical component (analyte) in an aqueous body fluid, such as whole blood or interstitial fluid.
- an aqueous body fluid such as whole blood or interstitial fluid.
- the present invention relates to a dry reagent test device from which an analyte presence and/or concentration is determined by use of an instrument and a disposable capillary reagent carrier and acquisition device.
- a common use of such test devices is for determination of glucose level in blood by diabetics.
- reagent strip containing a dry chemistry reagent system, that is, a system in which the wet chemistries are imbibed into an absorbent or bibulous medium, dried, and later reconstituted by fluid from the test sample.
- the reagent strips contain an indicator which changes color, depending on the presence or concentration of a particular analyte in a biological fluid applied to the strip. These strips may be read visually by reference to a color standard or colorimetrically by instrument calibrated or programmed to detect a certain color.
- strips use reduction chemistries, more commonly they involve an oxidizable dye or dye couple.
- Some of the strips include an enzyme, such as glucose oxidase, which is capable of oxidizing glucose to gluconic acid and hydrogen peroxide. They also contain an oxidizable dye and a substance having peroxidative activity, which is capable of selectively catalyzing oxidation of the oxidizable dye in the presence of hydrogen peroxide. (See, for example, U.S. Pat. No. 4,935,346 , to Phillips et al.) Examples of these devices, in addition to those used to test blood glucose, include tests for cholesterol, triglycerides,
- Dry chemistry reagent strips incorporating enzyme-based compositions are used daily by millions of diabetics to determine blood glucose concentrations.
- the NIH sponsored study, the Diabetes Complications and Control Trial demonstrated conclusively that careful control of blood glucose levels can significantly reduce the incidence of serious complications of diabetes such as vision loss and kidney malfunction.
- Most diabetics must test themselves periodically in order to make appropriate adjustments to their diet or medication. It is thus especially important for diabetics to have rapid, inexpensive, and accurate reagent strips for glucose determination.
- the embodiment of dry chemistry reagent systems in test strips enable simple yet effective analytical protocols.
- Phillips et al. U.S. Pat. No. 4,935,346 discloses a system wherein a whole blood sample is applied to the device and indicator development occurs in the presence of the colored components of the sample. Measurements of the color change in indicator are made at two distinct wavelengths to eliminate the interferences from the presence of colored blood components.
- Terminello et al. U.S. Pat. No. 4,774,192, disclose a system in which the matrix is formed of an asymmetric material used to filter the red blood cells in the sample.
- the asymmetric material has a density gradient from one side to the other to progressively separate red blood cells from the fluids.
- Erikson, et al., U.S. Pat. No. 5,582,184 disclose a system which uses a small capillary tube, membrane, and infrared testing.
- Daffern et al. U.S. Pat. No. 4,994,238, disclose a test device that comprises an asymmetric reagent layer that has progressively finer filtration with increased distance from one surface toward the other surface.
- Castino et al., U.S. Patent No. 5,456,835 disclose the use of filters formed of ligand modified polymeric film such as polypropylene fibers and polyethersulfone fibers.
- U.S. Patents. No. 4,854,322 and 4,77,953 disclose a capillary filtration and collection device for long term monitoring of blood constituents which is implanted within the interstitial body space in fluid communication with blood capillaries.
- this invention provides a method of testing or analyzing body fluids for the presence or concentration of an analyte by using a porous matrix.
- the porous matrix is provide through the use of hollow fiber made from various polymers including polyethersulphone, polysulphone, polyurethane, and other hydrophilic polymers.
- the hollow fibers can be dual skin in that they have two skins; one on the inner diameter and one on the outer diameter with various pore structures connecting the two skins, or have a single inner diameter skin with an isotropic pore structure radiating outward.
- the pore size of these skins can be either similar or dissimilar, but preferably should not be less than 0.1 microns and less that 5 microns in size.
- the device comprises of a capillary system which is used to collect the sample of body fluid.
- the capillary is the hollow portion of the hollow fiber.
- the sample is wicked up the hollow fiber and wets out the fiber matrix which is used as the membrane for analysis.
- the use of a hollow fiber capillary tube to collect the sample permits the patient the ability to collect a very small sample from any body location thereby increasing the number of possible sampling sites and body fluids where the patient can extract a sample of body fluid.
- the apparatus comprises of a hydrophilic hollow fiber capillary. The matrix can then be tested using various techniques including infrared, electrochemical, or colormetric means.
- the hollow fiber capillary can be cast from a hydrophilic compound, or hydrophilic agents can be coated either prior to or during reagent application.
- the test method comprises applying a blood sample to the hollow fiber capillary by placing the hollow fiber capillary tube in communication with the sample and allowing it to wick up the tube and wet out the matrix of the hollow fiber. This permits the fluid to pass through the hollow fiber capillary spreading onto the matrix.
- the reading or measuring for the presence/concentration of the analyte being tested is accomplished by detecting the change in reflectance of the indicator reagent for a colormetric test which is imbibed into the matrix.
- the embodiments of the devices of the invention with the appropriate dry chemistry system in the matrix member can be used in test strips which may be read or measured in an electronic meter.
- Figure 1 is an isometric view of the hollow fiber capillary test device
- Figure 2 is an isometric view of the hollow fiber capillary device being read by a reflectance meter.
- the devices of the present invention are simpler to use and are easier and less costly to manufacture than most devices previously available. This is especially important for diabetics who rely on blood glucose testing multiple times per day to keep their disease under control. For many diabetics, the costs associated with blood glucose monitoring are significant, especially elderly diabetics on fixed incomes.
- Devices of various configurations and various uses based on the embodiments of the invention disclosed herein can be delivered to the diabetic patient, in a more cost effective manner.
- the ease of use and ability to capture the sample provide a means to use non traditional sites to extract a sample. This eliminates the need for the patient to continually use the finger tips as a sampling site.
- the convenience of these devices, coupled with more attractive pricing, will facilitate increased patient compliance with recommended testing routines and will result in improved overall health of diabetic patients.
- the hollow fiber capillary matrix used in the invention are preferably a polyethersulfone polymer which is cast to inherently have a microporous skin on one side and a porous matrix on the other side, such as the Gelman membrane.
- a matrix of polysulphone, polyurethane, and other hydrophilic polymers, as well as hydrophobic polymers that are compatible with hydrophilic castings or surface treatments are illustrated by the embodiments which follow in the disclosure herein.
- the preferred method for providing a test area geometry is to utilize a hollow fiber as the capillary wick and reagent bearing member.
- the sample entry to the membrane test area which is the matrix of the hollow fiber is via the capillary center portion of the hollow fiber.
- the sample is wicked up the hollow fiber and wets out the surrounding matrix.
- the sample wets out the dried reagent imbibed in the matrix and the analyte reacts with the detection reagent to indicate the presence and/or concentration of the analyte. If used with an infrared detection system, the matrix does not contain an indicating chemistry.
- Hollow fibers are known in other applications. For example, see Schoonen et al., U.S. Patents 5,615,671 and 5,174,291, the disclosures of which are incorporated herein by reference.
- a wetting agent may be imbibed into the matrix of the hollow fiber to increase the hydrophilic properties of the hollow fiber to facilitate body fluid flow into the capillary tube and the matrix.
- High molecular weight polymeric oils work well as wetting agents.
- a preferred material is dimethylsiloxane ethylene oxide, part number PS073 from United Chemical Technologies.
- Other wetting agents include polypropylene glycol or polyethylene glycol.
- Separating agents can be impregnated into the matrix during or after the impregnation of test reagents.
- the specific compounds are selected to enhance the ability of the matrix to separate whole blood into red blood cells and clear fluid.
- the preferred matrix materials comprise a microporous polyethersulfone.
- the separating agents which can be impregnated into the membrane and or capillary wick may be selected from the following: poly vinyl sulfonic acid (PVSA), polyethylene glycol (PEG), polystyrene sulfonic acid (PSSA), hydroxypropyl cellulose (commercially available as Klucel TM), poly vinyl alcohol (PVA), polyvinylpyrrolidone (PVP), polyacrylic acid (PAA), water soluble salts, citrates, formates and sulfates, amino acids, chitosan (amino sugar), dextrans, citric acid, phytic acid and malic acid. These materials may be enhanced through combining with silica or clay.
- the chemical components can include equivalent materials which help to separate whole blood into red blood cells and relatively clear fluid.
- the indicating reagent mix must be capable of detecting the presence of the analyte.
- the analyte reacts with a specific oxidase enzyme and produces hydrogen peroxide. This strongly oxidative substance reacts with the indicator(s) present to produce a colored end product.
- the oxidase enzyme may be one of the following: glucose oxidase, cholesterol oxidase, uricase, alcohol oxidase, aldehyde oxidase or glycerophosphate oxidase. While the examples and preferred embodiments herein comprise glucose oxidase in the formulations, formulation changes required to utilize other oxidase enzymes are evident to one who is skilled in the art.
- the indicator chemistries which provide acceptable color generation when coated on the hollow fiber matrix may be chosen from 3-methyl-2-benzothiazolinone hydrazone hydrachloride (MBTH) combined with 3,3-dimethylaminobenzoic acid (DMAB), MBTH combined with 3,5-dichloro-2-hydroxybenzene-sulfonic acid (DCHBS); 4- aminoantipyrene (4-AAP) and 5-0xo-l-(p-sulfophenyl)-2-pyrazoline-3-carboxylic acid (OPSP); 4-AAP and n-(m-tolyl)-diethanolamine (NDA); 2,2'-azino-di (3- ethylbenzthiazoline) sulfonic acid (ABTS); 4AAP and 4-methoxynaphthol; pyrogallol red(PGR); bromopyrogallol red (BPR); acid green 25 (AG); MBTH and 8-an
- the MBTH-ANS system described by Yu in U.S. Patent No. 5,453,360 may be used in the methods and devices of this invention.
- One who is skilled in the art can formulate an acceptable chemistry based on the components disclosed herein and in the prior art.
- the above reagents will create a chemistry which can be read with a meter.
- the separation reagents, indicator reagents, oxidase enzymes, peroxidase enzymes, hematocrit adjuster, buffers, and chelators together with the dye system are impregnated in a membrane matrix selected from polyethersulfone.
- the issue of hematocrit level affecting the accuracy of test results is a substantial one for a test device.
- the following embodiment of this invention can be used to compensate for the hematocrit variation of whole blood.
- the instrument can be designed with additional sensors. These can either be electrical contacts or light sources and receivers (sensors) connected to analog signaling/conditioning circuit. These additional sensors can be implemented so that they inspect the capillary in the hollow fiber, one sensor at the beginning of the channel and one at the end.
- Whole blood is applied to the capillary.
- the test device capillary is translucent when using light sensors and the movement of whole blood is timed between sensors. The time that the blood takes to travel up the capillary is an indication of the hematocrit of the blood, and that information is used to correct any shift in reflectance readings of the instrument caused by the hematocrit level.
- the hollow fiber capillary test device 1 is shown in figure 1.
- the hollow core 2 of the fiber acts as a capillary.
- the second component is the matrix 3 or filtering media which contains the dried indicating reagent 4.
- This hollow fiber sits in an injected molded housing, preferably having an optical cavity housing 5 which provides the structural support for the hollow fiber.
- Figure 2 shows the device described in Figure 1 in which the indicating reagent is in the reacted state after absorbing the sample.
- the LED 8 and photo detector 9 read the color change through the hole 10 in injection molded part 5.
- the molded optical cavity housing can be designed to focus light from an LED onto the hollow fiber, or focus light emitted from the hollow fiber onto a detector, such as a photo diode detector.
- a two LED system can be utilized.
- One LED system can be selected to monitor the intensity of a dye impregnated into the fiber with the chemistry, while the other LED would monitor the color produced by the analyte of interest.
- the two wave length system of Phillips, et al., U.S. Patent No. 4,935,346 could also be used to correct for hemoglobin or blood, in the case where whole blood is the sample.
- the matrix material 3 will generally be in the ranges of about 0.1 microns to 5 microns in pore size. It will be recognized by those skilled in the art that the inside diameter of the fiber, the thickness of the matrix and the reagent used according to this invention may vary according to the desired use.
- the devices of this invention are conveniently made into test devices of convenient size and configuration for use in instruments or meters which are adapted to measure the color or other indication provided by the device. They are extremely useful for creating test devices which use small sample volumes. The complete sample is used by the device in that the fluid wicked up the hollow fiber is absorbed by the surrounding matrix and the indicating reaction takes place in this matrix.
- Example B Test Reagents Reagent lb 20 ml water
- citric acid (a buffering agent). Adjust the pH of the citric acid solution with NaOH to a value of 4.25.
- Reagent 2b 10 ml of a mixture of 3 parts by volume water and 7 parts by volume isopropyl alcohol 13 mg MBTH-S
- a piece of polyethersulfone hollow fiber is imbibed with reagent la; and the material is dried.
- the hollow fiber is then coated with reagent 2a in the same fashion and dried.
- the hollow fiber is then assembled into a test device as shown in Figure 1.
- Whole blood is applied to the capillary opening and the glucose level is read from side wall of the hollow fiber based on the indicator response in the test zone.
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98950861A EP1025442B1 (en) | 1997-09-30 | 1998-09-30 | Analytical device with capillary reagent carrier |
JP2000514135A JP2001518622A (en) | 1997-09-30 | 1998-09-30 | Analyzer using capillary reagent carrier |
DE69803310T DE69803310T2 (en) | 1997-09-30 | 1998-09-30 | ANALYTICAL DEVICE WITH CAPILLARY REAGENT |
AU96794/98A AU9679498A (en) | 1997-09-30 | 1998-09-30 | Analytical device with capillary reagent carrier |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6045897P | 1997-09-30 | 1997-09-30 | |
US60/060,458 | 1997-09-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999017117A1 true WO1999017117A1 (en) | 1999-04-08 |
Family
ID=22029619
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/020736 WO1999017117A1 (en) | 1997-09-30 | 1998-09-30 | Analytical device with capillary reagent carrier |
Country Status (6)
Country | Link |
---|---|
US (1) | US6544474B2 (en) |
EP (1) | EP1025442B1 (en) |
JP (1) | JP2001518622A (en) |
AU (1) | AU9679498A (en) |
DE (1) | DE69803310T2 (en) |
WO (1) | WO1999017117A1 (en) |
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GB2397375A (en) * | 2003-01-14 | 2004-07-21 | Hypoguard Ltd | Measuring analyte concentration in a fluid sample by illuminating the sample at two wavelengths |
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US8071384B2 (en) | 1997-12-22 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Control and calibration solutions and methods for their use |
US20050103624A1 (en) | 1999-10-04 | 2005-05-19 | Bhullar Raghbir S. | Biosensor and method of making |
US6540675B2 (en) | 2000-06-27 | 2003-04-01 | Rosedale Medical, Inc. | Analyte monitor |
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US7004928B2 (en) * | 2002-02-08 | 2006-02-28 | Rosedale Medical, Inc. | Autonomous, ambulatory analyte monitor or drug delivery device |
US20030171696A1 (en) * | 2002-03-05 | 2003-09-11 | Bayer Healthcare, Llc | Minimum invasive optical format with integrated lance |
US7052652B2 (en) | 2003-03-24 | 2006-05-30 | Rosedale Medical, Inc. | Analyte concentration detection devices and methods |
JP2004343275A (en) * | 2003-05-14 | 2004-12-02 | Murata Mach Ltd | Image processing system and scanner |
US7311812B2 (en) * | 2003-05-30 | 2007-12-25 | Abbott Laboratories | Biosensor |
US7718439B2 (en) | 2003-06-20 | 2010-05-18 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US7645421B2 (en) | 2003-06-20 | 2010-01-12 | Roche Diagnostics Operations, Inc. | System and method for coding information on a biosensor test strip |
US8206565B2 (en) | 2003-06-20 | 2012-06-26 | Roche Diagnostics Operation, Inc. | System and method for coding information on a biosensor test strip |
US7488601B2 (en) | 2003-06-20 | 2009-02-10 | Roche Diagnostic Operations, Inc. | System and method for determining an abused sensor during analyte measurement |
US8071030B2 (en) * | 2003-06-20 | 2011-12-06 | Roche Diagnostics Operations, Inc. | Test strip with flared sample receiving chamber |
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US8058077B2 (en) | 2003-06-20 | 2011-11-15 | Roche Diagnostics Operations, Inc. | Method for coding information on a biosensor test strip |
US7645373B2 (en) | 2003-06-20 | 2010-01-12 | Roche Diagnostic Operations, Inc. | System and method for coding information on a biosensor test strip |
US8679853B2 (en) * | 2003-06-20 | 2014-03-25 | Roche Diagnostics Operations, Inc. | Biosensor with laser-sealed capillary space and method of making |
US8148164B2 (en) | 2003-06-20 | 2012-04-03 | Roche Diagnostics Operations, Inc. | System and method for determining the concentration of an analyte in a sample fluid |
US7452457B2 (en) | 2003-06-20 | 2008-11-18 | Roche Diagnostics Operations, Inc. | System and method for analyte measurement using dose sufficiency electrodes |
WO2005078118A1 (en) | 2004-02-06 | 2005-08-25 | Bayer Healthcare Llc | Oxidizable species as an internal reference for biosensors and method of use |
US7569126B2 (en) | 2004-06-18 | 2009-08-04 | Roche Diagnostics Operations, Inc. | System and method for quality assurance of a biosensor test strip |
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JP2016522070A (en) | 2013-06-21 | 2016-07-28 | インテュイティ メディカル インコーポレイテッド | Analyte monitoring system using audible feedback |
DK3126486T3 (en) | 2014-04-02 | 2019-10-14 | Chembio Diagnostic Systems Inc | IMMUNASSAY USING THE Catch Conjugate |
US20160116466A1 (en) | 2014-10-27 | 2016-04-28 | Chembio Diagnostic Systems, Inc. | Rapid Screening Assay for Qualitative Detection of Multiple Febrile Illnesses |
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US5387526A (en) * | 1990-09-11 | 1995-02-07 | General Atomics | Coated capillary tube for the controlled release of reagent |
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1998
- 1998-09-30 DE DE69803310T patent/DE69803310T2/en not_active Expired - Fee Related
- 1998-09-30 EP EP98950861A patent/EP1025442B1/en not_active Expired - Lifetime
- 1998-09-30 AU AU96794/98A patent/AU9679498A/en not_active Abandoned
- 1998-09-30 JP JP2000514135A patent/JP2001518622A/en active Pending
- 1998-09-30 WO PCT/US1998/020736 patent/WO1999017117A1/en active IP Right Grant
-
2001
- 2001-01-05 US US09/755,362 patent/US6544474B2/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991011709A1 (en) * | 1990-01-29 | 1991-08-08 | Applied Biosystems, Inc. | High-viscosity polymer matrix and methods |
US5453360A (en) * | 1992-02-03 | 1995-09-26 | Lifescan, Inc. | Oxidative coupling dye for spectrophotometric quantitive analysis of analytes |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2397375A (en) * | 2003-01-14 | 2004-07-21 | Hypoguard Ltd | Measuring analyte concentration in a fluid sample by illuminating the sample at two wavelengths |
Also Published As
Publication number | Publication date |
---|---|
EP1025442A1 (en) | 2000-08-09 |
DE69803310D1 (en) | 2002-02-21 |
JP2001518622A (en) | 2001-10-16 |
EP1025442B1 (en) | 2001-11-21 |
US20010001034A1 (en) | 2001-05-10 |
AU9679498A (en) | 1999-04-23 |
DE69803310T2 (en) | 2002-08-14 |
US6544474B2 (en) | 2003-04-08 |
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