WO1999023097A1 - Substituted porphyrins - Google Patents
Substituted porphyrins Download PDFInfo
- Publication number
- WO1999023097A1 WO1999023097A1 PCT/US1998/023287 US9823287W WO9923097A1 WO 1999023097 A1 WO1999023097 A1 WO 1999023097A1 US 9823287 W US9823287 W US 9823287W WO 9923097 A1 WO9923097 A1 WO 9923097A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- compound
- pyp
- group
- independently
- manganese
- Prior art date
Links
- 0 CC1C(C)C*(C)(*)*C1 Chemical compound CC1C(C)C*(C)(*)*C1 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
- A61K31/409—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil having four such rings, e.g. porphine derivatives, bilirubin, biliverdine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/04—Drugs for disorders of the respiratory system for throat disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
Definitions
- the present invention relates, in general, to a method of modulating physiological and pathological processes and, in particular, to a method of modulating cellular levels of oxidants and thereby processes in which such oxidants are a participant.
- the invention also relates to compounds and compositions suitable for use in such methods .
- Oxidants are produced as part of the normal metabolism of all cells but also are an important component of the pathogenesis of many disease processes.
- Reactive oxygen species for example, are critical elements of the pathogenesis of diseases of the lung, the central nervous system and skeletal muscle.
- Oxygen free radicals also play a role in modulating the effects of nitric oxide (NO-) . In this context, they contribute to the pathogenesis of vascular disorders, inflammatory diseases and the aging process.
- NO- nitric oxide
- a critical balance of defensive enzymes against oxidants is required to maintain normal cell and organ function.
- Superoxide dismutases are a family of metalloenzymes that catalyze the intra- and extracellular conversion of O2- into H2O2 plus O2.
- Mammals produce three distinct SODs .
- One is a dimeric copper- and zinc-containing enzyme (CuZn SOD) found in the cytosol of all cells.
- a second is a tetrameric manganese-containing SOD (Mn SOD) found within mitochondria, and the third is a tetrameric, glycosylated, copper- and zinc-containing enzyme (EC-SOD) found in the extracellular fluids and bound to the extracellular matrix.
- CuZn SOD dimeric copper- and zinc-containing enzyme
- Mn SOD tetrameric manganese-containing SOD
- EC-SOD tetrameric, glycosylated, copper- and zinc-containing enzyme
- the present invention relates generally to low molecular weight porphyrin compounds suitable for use in modulating intra- and extracellular processes in which superoxide radicals, or other oxidants such as hydrogen peroxide or peroxynitrite, are a participant.
- the compounds and methods of the invention find application in various physiologic and pathologic processes in which oxidative stress plays a role.
- the present invention relates to a method of modulating intra- or extracellular levels of oxidants such as superoxide radicals, hydrogen peroxide, peroxynitrite, lipid peroxides, hydroxyl radicals and thiyl radicals. More particularly, the invention relates to a method of modulating normal or pathological processes involving superoxide radicals, hydrogen peroxide, nitric oxide or peroxynitrite, using low molecular weight antioxidants, and to methine (ie, -neso) substituted porphyrins suitable for use in such a method.
- the substituted porphyrins are also expected to have activity as antibacterial and antiviral agents, and as ionophores and chemotherapeutics .
- benzoic acid tetrakis-(4-benzoic acid) porphyrin (TBAP)
- TBAP tetrakis-(4-benzoic acid) porphyrin
- R 2 is either a hydrogen (H) or a
- E analog class is represented by trolox.
- C) The flavanoid class is represented by trolox.
- Rat brain homogenates Rat brain homogenates were incubated for various conditions
- TBARS thiobarbituric acid reactive species
- Rat brain iron/ascorbate mediated oxidation of rat brain homogenates.
- Rat brain homogenates were mediated oxidation of rat brain homogenates. Rat brain homogenates were
- Ratione inhibit iron/ascorbate mediated oxidation of rat brain homogenates.
- the present invention relates to methods of protecting against the deleterious effects of oxidants, particularly, superoxide radicals, hydrogen peroxide and peroxynitrite, and to methods of preventing and treating diseases and disorders that involve or result from oxidant stress.
- the invention also relates methods of modulating biological processes involving oxidants, including superoxide radicals, hydrogen peroxide, nitric oxide and peroxynitrite.
- the invention further relates to compounds and compositions, including low molecular weight antioxidants (eg mimetics of scavengers of reactive oxygen species, including mimetics of SODs, catalases and peroxidases) and formulations thereof, suitable for use in such methods .
- Mimetics of scavengers of reactive oxygen species appropriate for use in the present methods include methine (ie meso) substituted porphines, or pharmaceutically acceptable salts thereof.
- the invention includes both metal-free and metal-bound porphines.
- metal-bound porphines manganic derivatives of methine (meso) substituted porphines are preferred, however, metals other than manganese, such as iron (II or III), copper (I or II), cobalt (II or III), or nickel (I or II), can also be used.
- the metal selected can have various valence states, for example, manganese II, III or V can be used.
- Zinc (II) can also be used even though it does not undergo a valence change and therefore will not directly scavenge superoxide.
- the choice of the metal can affect selectivity of the oxygen species that is scavenged.
- Iron-bound porphines for example, can be used to scavenge NO- while manganese- bound porphines cannot. These metal bound porphines scavenge peroxynitrite; iron, nickel and cobalt bound porphines tend to have the highest reactivity with peroxynitrite .
- Preferred mimetics of the invention are of Formula I or II:
- R is C1-C3 alkyl, preferably, C_,-C 4 alkyl, more preferably, methyl, ethyl or isopropyi, most preferably methyl.
- This mimetic can also be present metal-free or bound to a metal other than Mn.
- Ail atropcisomers of the above are within the scope of the invention, present in isolated form or as a mixture of at least two. Atropoisomers wherein at least 3, preferably 4, of the R groups are above the porphyrin ring plane can be particularly advantageous .
- One or more of the pyrrole rings of the porphyrin of Formula I or II can be substituted at any or all beta carbons, ie: 2, 3, 7, 8, 12, 13, 17 or 18.
- substituent 1 ., designated P can be an electron withdrawing group, for example, each P can, independently, be a N0 2 group, a halogen (eg Cl, Br or F) , a nitrile, a vinyl group, or a formyl group.
- halogen eg Cl, Br or F
- Each P can, independently, also be hydrogen.
- P is formyl, it is preferred that there be not more than 2 (on non adjacent carbons), more preferably 1, the remaining P's being hydrogen.
- P is NO2, it is preferred that there be not more than 4 (on non adjacent carbons), more preferably 1 or 2, the remaining P's being hydrogen.
- Mimetics suitable for use in the present methods can be selected by assaying for SOD, catalase and/or peroxidase activity and stability. Mimetics can also be screened for their ability to inhibit lipid peroxidation in tissue homogenates using iron and ascorbate to initiate the lipid peroxidation and measuring the formation of thiobarbituric acid reactive species (TBARS) (Ohkawa et al, Anal. Biochem. 95:351 (1979) and Yue et al, J. Pharmacol. Exp . Ther. 263:92 (1992)).
- TBARS thiobarbituric acid reactive species
- the selective, reversible and SOD-sensitive inactivation of aconitase by known 0 " 2 generators can be used as a marker of intracellular 0 " 2 generation.
- suitable mimetics can be selected by assaying for the ability to protect aconitase activity.
- SOD activity can be monitored in the presence and absence of EDTA using the method of McCord and Fridovich (J. Biol. Chem. 244:6049 (1969)).
- the efficacy of a mimetic can also be determined by measuring the effect of the mimetic on the aerobic growth of a SOD null E . coli strain versus a parental strain lacking the specific mutations.
- parental E . coli (AB1157) and SOD null E . coli . JI132
- M9 medium containing 0.2% casamino acids and 0.2% glucose at pH 7.0 and 37°C
- growth can be monitored in terms of turbidity followed at 700 nm.
- This assay can be made more selective for SOD mimetics by omitting the branched chain, aromatic and sulphur containing amino acids from the medium (glucose minimal medium (M9) , plus 5 essential amino acids) (see Example V) .
- Efficacy of active mimetics can also be assessed by determining their ability to protect mammalian cells against methylviologen (paraquat) -induced toxicity.
- rat L2 cells grown as described below and seeded into 24 well dishes can be pre-incubated with various concentrations of the SOD mimetic and then incubated with a concentration of methylviologen previously shown to produce an LC75 in control L2 cells.
- Efficacy of the mimetic can be correlated with a decrease in the methylviologen-induced LDH release
- mice can be randomized into 4 groups of 8 mice each to form a standard 2X2 contingency statistical model. Animals can be treated with either paraquat
- Lung injury can be assessed 48 hours after paraquat treatment by analysis of bronchoalveolar lavage fluid (BALF) damage parameters (LDH, protein and
- Lungs from 2 mice of each group can be instillation-fixed with 4% paraformaldehyde and processed for histopathology at the light microscopic level .
- Catalase activity can be monitored by measuring absorbance at 240nm in the presence of hydrogen peroxide (see Beers and Sizer, J. Biol. Chem. 195:133 (1952)) or by measuring oxygen evolution with a Clark oxygen electrode (Del Rio et al, Anal. Biochem. 80:409 (1977)).
- Peroxidase activity can be measured spectrophotometrically as previously described by Putter and Becker: Peroxidases. In: Methods of Enzymatic
- Active mimetics can be tested for toxicity in mammalian cell culture by measuring lactate dehydrogenase (LDH) release.
- LDH lactate dehydrogenase
- Biol. 59:160a (1973)) can be grown in Ham's F-12 medium with 10% fetal calf serum supplement at pH 7.4 and 37"C; cells can be seeded at equal densities in 24 well culture dishes and grown to approximately 90% confluence; SOD mimetics can be added to the cells at -.log doses (eg micromolar doses in minimal essential medium (MEM)) and incubated for 24 hours. Toxicity can be assessed by morphology and by measuring the release of the cytosolic injury marker, LDH (eg on a thermokinetic plate reader) , as described by Vassault (In: Methods of Enzymatic Analysis, Bergmeyer (ed) pp.
- LDH cytosolic injury marker
- One specific embodiment of the present invention relates to a method of regulating NO- levels by targeting the above-described porphines to strategic locations.
- NO- is an intercellular signal and, as such,
- NO- must traverse the extracellular matrix to exert its effects. NO-, however, is highly sensitive to inactivation mediated by 0 2 ⁇ present in the extracellular spaces.
- the methine (meso) substituted porphyrins of the invention can increase bioavalability of NO ' by preventing its degradation by 0 2 " .
- the mimetics of the invention are used as catalytic scavengers of reactive oxygen species to protect against ischemia reperfusion injuries associated with myocardial infarction, stroke, acute head trauma, organ reperfusion following transplantation, bowel ischemia, hemorrhagic shock, pulmonary infarction, surgical occlusion of blood flow, and soft tissue injury.
- the mimetics can further be used to protect against skeletal muscle reperfusion injuries.
- the mimetics can also be used to protect against damage to the eye due to sunlight (and to the skin) as well as glaucoma, and macular degeneration in the eye.
- the mimetics can also be used to protect against and/or treat cataracts.
- the mimetics can also be used to protect against and/or treat inflammatory diseases of the skin (e.g., psoriasis) . Diseases of the bone are also amenable to treatment with the mimetics. Further, connective tissue disorders associated with defects in collagen synthesis or degradation can be expected to be susceptible to treatment with the present mimetics, as should the generalized deficits of aging.
- the mimetics of the invention can be used as catalytic scavengers of reactive oxygen species to increase the very limited storage viability of transplanted hearts, kidneys, skin and other organs and tissues.
- the invention also provides methods of inhibiting damage due to autoxidation of substances resulting in the formation of 0 2 ⁇ including food products, pharmaceuticals, stored blood, etc.
- the mimetics of the invention are added to food products, pharmaceuticals, stored blood and the like, in an amount sufficient to inhibit or prevent oxidation damage and thereby to inhibit or prevent the degradation associated with the autoxidation reactions. (For other uses of the mimetics of the invention, see USP 5,227,405).
- the amount of mimetic to be used in a particular treatment or to be associated with a particular substance can be determined by one skilled in the art.
- the mimetics of the invention can be used to scavenge hydrogen peroxide and thus protect against formation of the highly reactive hydroxyl radical by interfering with Fenton chemistry (Aruo a and Halliwell, Biochem. J. 241:273 (1987); Mello Filho et al, Biochem. J. 218:273 (1984); Rush and Bielski, J. Phys . Chem. 89:5062 (1985)).
- the mimetics of the invention may also be used to scavenge peroxynitrite, as demonstrated indirectly by inhibition of the oxidation of dihydrorhodamine 123 to rhodamine 123 and directly by accelerating peroxynitrite degradation by stop flow analysis.
- diseases/disorders appropriate for treatment using the mimetics of the present invention include diseases of the central nervous system (including AIDS dementia, stroke, amyotrophic lateral sclerosis (ALS), Parkinson's disease and Huntington ' s disease) and diseases of the musculature (including diaphramic diseases (eg respiratory fatigue in emphysema, bronchitis and cystic fibrosis) , cardiac fatigue of congestive heart failure, muscle weakness syndromes associated with myopathies, ALS and multiple sclerosis) .
- AIDS dementia including AIDS dementia, stroke, amyotrophic lateral sclerosis (ALS), Parkinson's disease and Huntington ' s disease
- diseases of the musculature including diaphramic diseases (eg respiratory fatigue in emphysema, bronchitis and cystic fibrosis) , cardiac fatigue of congestive heart failure, muscle weakness syndromes associated with myopathies, ALS and multiple sclerosis) .
- NMDA neuronal dihydroxyaspartate
- NMDA neuronal calcium concentrations
- NO- oxygen free radicals and nitric oxide
- Interactions between oxygen free radicals and NO- have been shown to contribute to neuronal cell death.
- Well-established neuronal cortical culture models of NMDA-toxicity have been developed and used as the basis for drug development. In these same systems, the mimetics of the present invention inhibit NMDA-induced injury.
- 0 ⁇ 2 radicals are an obligate step in the intracellular events culminating in excitotoxic death of cortical neurons and further demonstrate that the mimetics of the invention can be used to scavenge 0 ⁇ 2 radicals and thereby serve as protectants against excitotoxic injury.
- the present invention also relates to methods of treating AIDS.
- the NfKappa B promoter is used by the HIV virus for replication. This promoter is redox sensitive, therefore, an antioxidant can regulate this process. This has been previously shown for two metalloporphyrins distinct from those of the present invention (Song et al, Antiviral Chem. And Chemother.
- the invention also relates to methods of treating arthritis, systemic hypertension, atherosclerosis, edema, septic shock, pulmonary hypertension, including primary pulmonary hypertension, impotence, MED, infertility, endometriosis, premature uterine contractions, microbial infections, gout and in the treatment of Type I and Type II diabetes mellitus.
- the mimetics of the invention can be used to ameliorate the toxic effects associated with endotoxin, for example, by preserving vascular tone and preventing multi-organ system damage.
- Inflammations particularly inflammations of the lung, are amenable to treatment using the present invention (note particularly the inflammatory based disorders of asthma, ARDS including oxygen toxicity, pneumonia (especially AIDS-related pneumonia) , cystic fibrosis, chronic sinusitis and autoimmune diseases
- EC-SOD is localized in the interstitial spaces surrounding airways and vasculature smooth muscle cells.
- EC-SOD and 0 2 ⁇ mediate the antiinflammatory - proinflammatory balance in the alveolar septum. NO- released by alveolar septal cells acts to suppress inflammation unless it reacts with 0 2 ⁇ to form ONOO " .
- EC-SOD tips the balance in the alveolar septum against inflammation.
- nitric oxide is a neurotransmitter involved in long-term memory potentiation.
- EC-SOD knocked-out mouse model Carlsson et al, Proc. Natl. Acad. Sci . USA 92:6264 (1995) ) , it can be shown that learning impairment correlates with reduced superoxide scavenging in extracellular spaces of the brain. Reduced scavenging results in higher extracellular 0 ⁇ 2 levels.
- 0 " 2 is believed to react with nitric oxide thereby preventing or inhibiting nitric oxide-medicated neurotransmission and thus long-term memory potentiation.
- the mimetics of the invention can be used to treat dementias and memory/learning disorders.
- compositions suitable for use in the present methods include the active agent (mimetic) together with a pharmaceutically acceptable carrier, excipient or diluent.
- the composition can be present in dosage unit form for example, tablets, capsules or suppositories.
- the composition can also be in the form of a sterile solution suitable for injection or nebulization .
- Compositions can also be in a form suitable for opthal ic use.
- the invention also includes compositions formulated for topical administration, such compositions taking the form, for example, of a lotion, cream, gel or ointment.
- the concentration of active agent to be included in the composition can be selected based on the nature of the agent, the dosage regimen and the result sough .
- the dosage of the composition of the invention to be administered can be determined without undue experimentation and will be dependent upon various factors including the nature of the active agent, the route of administration, the patient, and the result sought to be achieved.
- a suitable dosage of mimetic to be administered, for example, IV or topically can be expected to be in the range of about 0.01 to 100 g/kg/day, preferably 0.1 to 10 mg/kg/day.
- For aerosol administration it is expected that doses will be in the range of 0.01 to 1.0 mg/kg/day.
- Suitable doses of mimetics will vary, for example, with the mimetic and with the result sought.
- Faulkner et al J. Biol. Chem.
- H2 -2-PyP was bought from MidCentury Chemicals and the purity checked in terms of elemental analysis (see below) .
- Iodoethane, 1-iodobutane, anhydrous manganese chloride (MnCl2), MnCl2- H2 ⁇ , tetrabutylammonium chloride (TBA) and ammonium hexafluorophosphate (PFgNH 4 ) were purchased from Aldrich.
- the metallation was performed in water at room temperature.
- the porphyrin to metal ratio was 1:5 in the case of meta and ortho isomers and 1:14 in the case of para isomer.
- the solid MnCl 2 x 4 H 2 0 Aldrich
- the metallation was completed inside an hour in the cases of all three isomers.
- MnTM-2-PyP 5+ and MnTM-3-PyP 5+ 300 mg of the metal-free ligand, either H 2 TM-2-PyP 4+ or H 2 TM-3-
- the excess of metal was eliminated as follows for all three ( ortho, meta and ra isomers of MnTMPyP 5+ .
- the MnTMPyP 5+ was precipitated as PF 6 " salt by adding 50- fold excess of NH 4 PF ⁇ .
- Dry PF 6 " salt of MnTMPyP 5" was then dissolved in acetone (370 mg in 100 L acetone) and 1 g of tetrabutylammoniu chloride added.
- the precipitate was washed with acetone and dried overnight in vacuum at room temperature. In order to obtain a pure compound, the procedure was repeated.
- Metallation was performed in methanol as well. In addition, when performed in water, the metal: ligand ratio varied from 1:5, to 1:14 to 1:100. Under all conditions, the given molar absorptivities were obtained. The calculations were based on the metal-free ligands that were analyzed prior to metallation. The molar absorptivities of the metal-free ligands were consistent with literature as well as their elemental analyses .
- the superoxide dismutase activity of the mimetics of the invention depends on a number of factors, including thermodynamic factors (eg the metal-centered redox-potential see Fig. 1)), and kinetic factors (eg electrostatic facilitation) .
- thermodynamic factors eg the metal-centered redox-potential see Fig. 1
- kinetic factors eg electrostatic facilitation
- the ortho compound "3" proves to be more than an order of magnitude more active than the para compound "1” (see Fig. 2 (note also Table 2 where "2" is the meta compound and "4" and "5" are ortho compounds that carry 4 ethyl or 4 butyl groups, respectively) ) .
- the activity in vivo of the mimetics of the invention can be tested on an E . coli strain deleted of the genes coding for both the MnSOD and FeSOD.
- the efficacy of a mimetic is determined by measuring the effect of the mimetic on the aerobic growth of a SOD null E . coli strain versus a parental strain. Specifically, parental E. coli (AB1157) and SOD null E. coli . (JI132) are grown in M9 medium containing 0.2% casamino acids and 0.2% glucose at pH 7.0 and 37°C; growth is monitored in terms of turbidity followed at 700 nm.
- This assay is made more selective for SOD mimetics by omitting the branched chain, aromatic and sulphur containing amino acids from the medium (glucose minimal medium (M9), plus 5 essential amino acids).
- the increase in activity by the " ortho effect” was confirmed in that, under these growth conditions, SOD null cells cultured in the presence of compound "1” did not show an increase in A 70 o while such cells cultured in the presence of compounds "3" and "4" (as well as "2”) did.
- ETS tetrabutylammonium chloride (98%)
- TBAC tetrabutylammonium chloride
- cytochrome c cytochrome c, xanthine, ethylenedinitrilotetraacetic acid (EDTA), NN-
- dichloromethane (HPLC grade) were from Mallinckrodt, and used without further
- Xanthine oxidase was supplied by R.D. Wiley (Waud et al, Arch.
- MALDI-TOFMS assisted laser desorption/ionization - time of flight -
- H 2 Cl,T-2-PyP 50 mg (8.1 x 10 "5 moles) of H 2 T-2-PyP was refluxed in
- T-2-PyP was purified again using the same system leading to 16 mg of a black
- ETS toluenesulfonate
- TBAC tetrabutylammonium chloride
- Electrochemistry The electrochemical characterization was performed as
- Cytochrome c concentration was at least 10 3 -fold higher than the concentrations of
- reaction can be followed by TLC (silica gel) using a mixture
- Table3 H 2 Cl,T-2-PyP (x 1 to 4): R, ; Soret band data and yields with 4 and 6 equivalents of ⁇ CS.
- ETS ethyl-/?-toluenesulfonate
- each compound is in fact a mixture of four
- M*'s"' representing the rate constant for the superoxide dismutation reaction.
- SOD-like activity per mole of MnCl 4 TE-2-PyP 5+ is approximately 2-, 7- and 100-
- pKa as a measure of the ligand-proton stability, is in turn a measure of the metal-
- the tetrachloro-compound is expected to be of
- MnCl 4 TE-2-PyP 5+ was tested by measuring its SOD-like activity in the presence of
- SOD enzyme is a dimer of two identical subunits, and thus has two active sites
- chlorination is expected to stabilize the manganese in the 2+ redox state, and thus,
- Metalloporphyrins are Potent Inhibitors of Lipid Peroxidation
- hydrochloric acid 4,6-dihydroxy-2-mercaptopyrimidine (thiobarbituric acid),
- methylpyridinium-2-yl) porphyrin H 2 TM-2-PyP
- (+)-Rutin was purchased from
- Milli-Q Plus PF water Milli-Q Plus PF water (Millipore, Bedford, MA).
- the metalloporphyrins MnTBAP, CoTBAP and ZnTBAP were made
- MnTM-4-PyP, CoTM-4-PyP and ZnTM-4-PyP were used.
- MnTM-4-PyP was eluted with 0.01 N HCI after
- the Soret band for MnTM-4-PyP is at 463 nm with an extinction coefficient
- methylpyridinium-4-yl) porphyrin (MnOBTM-4-PyP) was synthesized as
- concentration was determined with the Coomassie Plus protein assay (Pierce, Rockford, IL) using bovine serum albumin as a standard.
- Rat brain homogenates (2 mg protein) were incubated with varying amounts
- brain homogenate was initiated by the addition of 0.1 ml of a freshly
- TBARS thiobarbituric acid reactive species
- metalioporphyrins could inhibit lipid peroxidation and to compare their
- TBARS thiobarbituric reactive species
- MnOBTM-4-PyP and MnTM-2-PyP that have the highest SOD activities, MnOBTM-4-PyP and MnTM-2-PyP,
- catalase up to concentrations of 1 mg/ml did not inhibit iron/ascorbate-initiated lipid peroxidation.
- Unit of SOD activity defined as the amount of compound that inhibits one half the reduction of cytochrome c or photoreduction of NBT.
- a wide range of metals can be covalently ligated by porphyrins and
- TMPyP tetrakis N-methylpyridyl porphyrin
- MnTM-2-PyP and MnTM-4-PyP differ structurally with respect to the
- porphyrin analogs results in loss of SOD activity.
- MnTM-2-PyP possesses a more positive redox potential than MnTM-
- livers were then rendered globally ischemic under normal thermic
- metalloporphyrin It has demonstrated excellent oxygen consumption and a normal
- Rats were anesthetized and a femoral vein and carotid artery were cannulated.
- Mn TM-2-PyP is being modulated by nitric oxide. Scavenging of superoxide in
- vascular walls would potentiate the effects of nitric oxide producing hypotension.
- TM-2-PyP is equivalent to 0.8 mg/kg whole body dose.
- Neonatal baboons were delivered prematurely by Caesarian section and then
- Mn TE-2-PyP can be used to treat oxidant stress in
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL13594998A IL135949A0 (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
CA2309154A CA2309154C (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
AT98956457T ATE238307T1 (en) | 1997-11-03 | 1998-11-03 | SUBSTITUTED PORPHYRINS |
EP98956457A EP1045851B1 (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
JP2000518967A JP2001521939A (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
DE69813898T DE69813898T2 (en) | 1997-11-03 | 1998-11-03 | SUBSTITUTED PORPHYRINES |
AU12979/99A AU737650B2 (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6411697P | 1997-11-03 | 1997-11-03 | |
US60/064,116 | 1997-11-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999023097A1 true WO1999023097A1 (en) | 1999-05-14 |
Family
ID=22053669
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/023287 WO1999023097A1 (en) | 1997-11-03 | 1998-11-03 | Substituted porphyrins |
Country Status (10)
Country | Link |
---|---|
US (4) | US6916799B2 (en) |
EP (1) | EP1045851B1 (en) |
JP (2) | JP2001521939A (en) |
AT (1) | ATE238307T1 (en) |
AU (1) | AU737650B2 (en) |
CA (1) | CA2309154C (en) |
DE (1) | DE69813898T2 (en) |
ES (1) | ES2198767T3 (en) |
IL (1) | IL135949A0 (en) |
WO (1) | WO1999023097A1 (en) |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000019993A2 (en) * | 1998-10-06 | 2000-04-13 | Albert Einstein College Of Medicine Of Yeshiva University | Methods and compositions for decreasing mitochondrial overproduction of reactive oxygen species in cells |
WO2001072327A2 (en) * | 2000-03-28 | 2001-10-04 | Universite Rene Descartes (Paris V) | Use of analogues of superoxide dismutase for treating hepatocellular insufficiencies |
EP1155019A1 (en) * | 1999-01-25 | 2001-11-21 | National Jewish Medical and Research Center | Substituted porphyrins |
US6479477B1 (en) | 1998-04-24 | 2002-11-12 | Duke University | Substituted porphyrins |
US6583132B1 (en) | 1993-10-15 | 2003-06-24 | Duke University | Oxidant scavengers |
WO2003051458A1 (en) * | 2001-12-14 | 2003-06-26 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1392328A2 (en) * | 2001-01-19 | 2004-03-03 | National Jewish Medical and Research Center | Cancer therapy |
EP1439842A1 (en) * | 2001-06-01 | 2004-07-28 | National Jewish Medical and Research Center | Oxidant scavengers for treatment of diabetes or use in transplantation or induction of immune tolerance |
WO2004069273A1 (en) * | 2003-02-05 | 2004-08-19 | Photobiochem N.V. | Use of a porphyrin compound fro the treatment of skin fungi |
EP1513537A1 (en) * | 2002-06-07 | 2005-03-16 | Duke University | Substituted porphyrins |
WO2005077269A1 (en) | 2004-02-09 | 2005-08-25 | Duke University | Substituted porphyrins |
EP1567166A2 (en) * | 2002-12-06 | 2005-08-31 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1581212A2 (en) * | 2002-12-06 | 2005-10-05 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
WO2005097123A3 (en) * | 2004-03-29 | 2005-12-22 | Inotek Pharmaceuticals Corp | Pyridyl-substituted porphyrin compounds and methods of use thereof |
EP2625180A2 (en) * | 2010-10-06 | 2013-08-14 | Aeolus Sciences, Inc. | Porphyrin treatment of neurodegenerative diseases |
US8598150B1 (en) | 2008-04-02 | 2013-12-03 | Jonathan R. Brestoff | Composition and method for affecting obesity and related conditions |
RU2506083C2 (en) * | 2008-05-23 | 2014-02-10 | Нэшнл Джуиш Хелт | Method of treating disorders associated with alkylating agent exposure |
US8987245B2 (en) | 2008-04-02 | 2015-03-24 | Jonathan R. Brestoff Parker | Composition and method for affecting obesity and related conditions |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001521939A (en) * | 1997-11-03 | 2001-11-13 | デューク・ユニバーシティー | Substituted porphyrins |
US20040137638A1 (en) | 2002-03-04 | 2004-07-15 | Slomczynska Urszula J. | Chromatography of metal complexes |
GB2397067B (en) * | 2002-12-23 | 2005-05-11 | Destiny Pharma Ltd | Porphin & azaporphin derivatives with at least one cationic-nitrogen-containing meso-substituent for use in photodynamic therapy & in vitro sterilisation |
JP4741205B2 (en) * | 2003-07-07 | 2011-08-03 | 真 湯浅 | Metalloporphyrin complex-embedded liposome, method for producing the same, and medicament using the same |
US7299082B2 (en) | 2003-10-31 | 2007-11-20 | Abbott Diabetes Care, Inc. | Method of calibrating an analyte-measurement device, and associated methods, devices and systems |
US7699964B2 (en) | 2004-02-09 | 2010-04-20 | Abbott Diabetes Care Inc. | Membrane suitable for use in an analyte sensor, analyte sensor, and associated method |
US8165651B2 (en) * | 2004-02-09 | 2012-04-24 | Abbott Diabetes Care Inc. | Analyte sensor, and associated system and method employing a catalytic agent |
GB2415372A (en) | 2004-06-23 | 2005-12-28 | Destiny Pharma Ltd | Non photodynamical or sonodynamical antimicrobial use of porphyrins and azaporphyrins containing at least one cationic-nitrogen-containing substituent |
EP1928467A4 (en) * | 2005-09-28 | 2009-11-04 | Inotek Pharmaceuticals Corp | N-benzyl substituted pyridyl porphyrin compounds and methods of use thereof |
US7885698B2 (en) | 2006-02-28 | 2011-02-08 | Abbott Diabetes Care Inc. | Method and system for providing continuous calibration of implantable analyte sensors |
HUE044234T2 (en) | 2006-10-12 | 2019-10-28 | Galera Labs Llc | Methods of treating oral mucositis |
US20080289053A1 (en) * | 2007-05-15 | 2008-11-20 | The Regents Of The University Of California | Methods and systems for identifying modulators of longevity |
US8608950B2 (en) * | 2009-12-30 | 2013-12-17 | Uop Llc | Process for removing metals from resid |
JP5823988B2 (en) * | 2011-01-31 | 2015-11-25 | 国立大学法人宇都宮大学 | Method for producing metal complex of compound having porphyrin type skeleton |
AU2012316397B2 (en) | 2011-09-26 | 2016-07-21 | Galera Labs, Llc | Methods for treatment of diseases |
WO2013130150A2 (en) | 2011-12-02 | 2013-09-06 | The Regents Of The University Of Colorado, A Body Corporate | Metalloporphyrin neurological treatments |
WO2015034778A1 (en) | 2013-09-03 | 2015-03-12 | Biomimetix J.V., Llc | Methods of treating erectile dysfunction |
CA2933166C (en) | 2013-12-31 | 2020-10-27 | Abbott Diabetes Care Inc. | Self-powered analyte sensor and devices using the same |
WO2015112586A1 (en) | 2014-01-22 | 2015-07-30 | Duke University | Methods of treating pruritus |
US10064871B2 (en) | 2014-01-22 | 2018-09-04 | Biomimetix Jv, Llc | Methods of treating skin disorders |
CN113402560A (en) | 2015-08-11 | 2021-09-17 | 加莱拉实验室有限责任公司 | Pentaazamacrocycle complexes with oral bioavailability |
IL295620B2 (en) | 2017-04-13 | 2024-01-01 | Galera Labs Llc | Combination cancer immunotherapy with pentaaza macrocyclic ring complex |
WO2018237249A1 (en) * | 2017-06-22 | 2018-12-27 | Biomimetix Jv, Llc | Methods, compositions, and kits for treating inflammatory skin conditions |
US11918549B2 (en) | 2017-08-25 | 2024-03-05 | AZ Solutions LLC | System and method for wound treatment and irrigation |
CA3076008A1 (en) | 2017-09-29 | 2019-04-04 | Duke University | Fluoro substituted porphyrin compounds, pharmaceutical compositions comprising the same, and methods of preparing and using the same |
CN111100133B (en) * | 2019-12-30 | 2020-12-01 | 云南大学 | Metalloporphyrin compound and preparation method and application thereof |
CA3218113A1 (en) * | 2021-05-04 | 2022-11-10 | Derek Hill | Treatment of lung and airway diseases and disorders |
Family Cites Families (89)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2951799A (en) | 1957-11-13 | 1960-09-06 | Monsanto Chemicals | Photoxidation processes using heterocyclic photosensitizers |
EP0127797B1 (en) | 1983-06-03 | 1987-06-16 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Labelled molecules for fluorescence immunoassays and processes and intermediates for their preparation |
US4829984A (en) | 1983-12-15 | 1989-05-16 | Gordon Robert T | Method for the improvement of transplantation techniques and for the preservation of tissue |
US4963367A (en) | 1984-04-27 | 1990-10-16 | Medaphore, Inc. | Drug delivery compositions and methods |
US5087438A (en) | 1984-07-03 | 1992-02-11 | Gordon Robert T | Method for affecting intracellular and extracellular electric and magnetic dipoles |
GB8429845D0 (en) | 1984-11-26 | 1985-01-03 | Efamol Ltd | Porphyrins & cancer treatment |
US4758422A (en) | 1985-01-04 | 1988-07-19 | Salutar Inc. | Ferrioxamine paramagnetic contrast agents for MR imaging |
US4657902A (en) | 1985-03-25 | 1987-04-14 | The Rockefeller University | Therapeutic use of tin mesoporphyrin |
WO1986006959A1 (en) | 1985-05-22 | 1986-12-04 | Liposome Technology, Inc. | Liposome inhalation method and system |
US5248603A (en) | 1985-09-03 | 1993-09-28 | Symbicom Aktiebolag | Superoxide dismutase |
DK402785D0 (en) | 1985-09-03 | 1985-09-03 | Syn Tek Ab | PROCEDURE FOR THE PREPARATION OF AN ENZYM |
US4866054A (en) | 1986-05-13 | 1989-09-12 | Chai-Tech Corporation | Antioxidant metallo-organic treatment of inflammation |
US4746735A (en) | 1986-11-21 | 1988-05-24 | The Dow Chemical Company | Regiospecific aryl nitration of meso-substituted tetraarylporphyrins |
US5162231A (en) | 1989-10-25 | 1992-11-10 | Cole Dean A | Method of using 5,10,15,20-tetrakis(carboxyphenyl)porphine for detecting cancers of the lung |
EP0282899B1 (en) | 1987-03-14 | 1996-01-10 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Human manganese superoxide dismutase (hMn-SoD) |
US5223538A (en) | 1987-03-31 | 1993-06-29 | Duke University | Superoxide dismutase mimic |
US5227405A (en) | 1987-03-31 | 1993-07-13 | Duke University | Superoxide dismutase mimic |
US4892941A (en) | 1987-04-17 | 1990-01-09 | Dolphin David H | Porphyrins |
US4851403A (en) | 1987-04-21 | 1989-07-25 | Johnson Matthey, Inc. | Radiation sensitizers |
US4885114A (en) | 1987-04-22 | 1989-12-05 | Barnes Engineering Co. | Metallized tetra((meso)-5-methyl-2-thiophene)porphines, platinum (5-bromo octaethylporphine) and optical filters containing same |
US5051337A (en) | 1987-06-10 | 1991-09-24 | Director-General Of Agency Of Industrial Science And Technology | Optical recording material |
US5162519A (en) | 1988-03-11 | 1992-11-10 | Efamol Holdings Plc | Porphyrins and cancer treatment |
GB8805849D0 (en) | 1988-03-11 | 1988-04-13 | Efamol Holdings | Porphyrins & cancer treatment |
DE3809671A1 (en) | 1988-03-18 | 1989-09-28 | Schering Ag | PORPHYRINE COMPLEX COMPOUNDS, METHOD FOR THE PRODUCTION THEREOF AND PHARMACEUTICAL AGENTS CONTAINING THEM |
US5284647A (en) | 1988-03-18 | 1994-02-08 | Schering Aktiengesellschaft | Mesotetraphenylporphyrin complex compounds, process for their production and pharmaceutical agents containing them |
US5109016A (en) | 1988-05-23 | 1992-04-28 | Georgia State University Foundation, Inc. | Method for inhibiting infection or replication of human immunodeficiency virus with porphyrin and phthalocyanine antiviral compositions |
US5192788A (en) | 1988-05-23 | 1993-03-09 | Georgia State University Foundation, Inc. | Porphyrin antiviral compositions |
FR2632187B1 (en) | 1988-06-02 | 1990-09-14 | Centre Nat Rech Scient | METALLOPORPHYRIN DERIVATIVES, THEIR PREPARATION, THEIR THERAPEUTIC APPLICATION AND THEIR USE FOR THE PREPARATION OF HYBRID MOLECULES |
US5171680A (en) | 1988-06-14 | 1992-12-15 | Chiron Corporation | Superoxide dismutase analogs having novel binding properties |
EP0414915B1 (en) | 1989-03-06 | 1996-06-12 | Suntory Limited | New superoxide dismutase |
JPH0341441A (en) * | 1989-07-07 | 1991-02-21 | Konica Corp | Silver halide photographic sensitive material improved in spot fault |
DK455789D0 (en) | 1989-09-15 | 1989-09-15 | Symbicom Ab | POLYPEPTIDE |
US5010073A (en) | 1989-10-05 | 1991-04-23 | The Rockefeller University | Use of liposomes as carriers for metalloporphyrins |
EP0424033A3 (en) | 1989-10-19 | 1991-07-31 | Pola Chemical Industries Inc | External skin preparation |
US5236915A (en) | 1990-05-31 | 1993-08-17 | Health Research, Inc. | Meso poly(4-sulfonatophenyl) porphines as MRI image enhancing agents |
EP0462836A3 (en) | 1990-06-20 | 1992-08-26 | Mitsui Toatsu Chemicals, Inc. | Recombinant vector plasmid capable of expressing human manganese superoxide dismutase, and process of producing this enzyme |
US5217966A (en) | 1990-09-13 | 1993-06-08 | The Regents Of The University Of California | Synthetic drug molecules that mimic metalloenzymes |
US5202317A (en) | 1990-09-13 | 1993-04-13 | The Regents Of The University Of California | Synthetic drug molecules that mimic metalloenzymes |
WO1992007935A1 (en) | 1990-11-01 | 1992-05-14 | The Scripps Research Institute | Glycosaminoglycan-targeted fusion proteins, their design, construction and compositions |
EP0557417A4 (en) | 1990-11-14 | 1994-06-08 | Uab Research Foundation | Compositions for reducing oxidative injury |
US5192757A (en) | 1990-12-20 | 1993-03-09 | Glaxo Inc. | Cobalt porphyrins |
GB9103991D0 (en) | 1991-02-26 | 1991-04-10 | Nat Res Dev | Molecular electronic devices |
FR2676738B1 (en) | 1991-05-22 | 1995-05-05 | Ir2M | NOVEL ORGANIC TRANSITION METAL WITH PORPHYRINIC STRUCTURE, THERAPEUTIC COMPOSITION CONTAINING SAME, IN PARTICULAR WITH HYPOGLYCEMIC ACTIVITY. |
JP3155552B2 (en) | 1991-07-19 | 2001-04-09 | モンサント カンパニー | Manganese complexes of nitrogen-containing macrocyclic ligands as effective catalysts for the asymmetric change of superoxide |
CA2072934C (en) | 1991-07-19 | 2007-08-28 | Karl William Aston | Manganese complexes of nitrogen-containing macrocyclic ligands effective as catalysts for dismutating superoxide |
US5262532A (en) | 1991-07-22 | 1993-11-16 | E.R. Squibb & Sons, Inc. | Paramagnetic metalloporphyrins as contrast agents for magnetic resonance imaging |
US5212300A (en) | 1991-09-12 | 1993-05-18 | Sun Company, Inc. (R&M) | Cyano- and polycyanometallo-porphyrins as catalysts for alkane oxidation |
US5976551A (en) | 1991-11-15 | 1999-11-02 | Institut Pasteur And Institut Nationale De La Sante Et De La Recherche Medicale | Altered major histocompatibility complex (MHC) determinant and method of using the determinant |
US5371199B1 (en) | 1992-08-14 | 1995-12-26 | Univ Pennsylvania | Substituted porphyrins porphyrin-containing polymers and synthetic methods therefor |
US5493017A (en) | 1992-08-14 | 1996-02-20 | The Trustees Of The University Of Pennsylvania | Ring-metalated porphyrins |
US5599924A (en) | 1992-08-14 | 1997-02-04 | Trustees Of The University Of Pennsylvania | Electron-deficient porphyrins and processes and intermediates for preparing same |
AU4846193A (en) | 1992-09-03 | 1994-03-29 | Regents Of The University Of California, The | Metallo porphyrin compositions |
US5403834A (en) | 1992-12-07 | 1995-04-04 | Eukarion, Inc. | Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease |
US5696109A (en) | 1992-12-07 | 1997-12-09 | Eukarion, Inc. | Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease |
US5834509A (en) | 1992-12-07 | 1998-11-10 | Eukarion, Inc. | Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease |
US6204259B1 (en) | 1993-01-14 | 2001-03-20 | Monsanto Company | Manganese complexes of nitrogen-containing macrocyclic ligands effective as catalysts for dismutating superoxide |
DE4305523A1 (en) | 1993-02-17 | 1994-08-18 | Diagnostikforschung Inst | Meso-tetraphenylporphyrin complexes, processes for their preparation and pharmaceutical compositions containing them |
US6417182B1 (en) | 1993-08-25 | 2002-07-09 | Anormed Inc. | Pharmaceutical compositions comprising metal complexes |
US5994339A (en) | 1993-10-15 | 1999-11-30 | University Of Alabama At Birmingham Research Foundation | Oxidant scavengers |
US5747026A (en) | 1993-10-15 | 1998-05-05 | University Of Alabama At Birmingham Research Foundation | Antioxidants |
US6127356A (en) | 1993-10-15 | 2000-10-03 | Duke University | Oxidant scavengers |
US5608054A (en) | 1993-12-29 | 1997-03-04 | Sun Company, Inc. (R&M) | Porphyrins and metal complexes thereof having haloalkyl side chains |
US5405369A (en) | 1994-01-25 | 1995-04-11 | Medical College Of Ohio | Photochemical ablation of gastro-intestinal tissue for augmentation of an organ |
US5604199A (en) | 1994-03-29 | 1997-02-18 | The Nemours Foundation | Method of treating fibrosis in skeletal muscle tissue |
CA2189528A1 (en) | 1994-05-13 | 1995-11-23 | Michael Keith Stern | Methods of use for peroxynitrite decomposition catalysts, pharmaceutical compositions therefor |
US6245758B1 (en) | 1994-05-13 | 2001-06-12 | Michael K. Stern | Methods of use for peroxynitrite decomposition catalysts, pharmaceutical compositions therefor |
US5563132A (en) | 1994-06-21 | 1996-10-08 | Bodaness; Richard S. | Two-step cancer treatment method |
FR2723694B1 (en) * | 1994-08-19 | 1996-09-27 | Oreal | USE OF SALTS OF CATIONIC PORPHINE DERIVATIVES AS PHOTOSENSITIZERS OF GRAM-NEGATIVE BACTERIA |
WO1996009053A1 (en) | 1994-09-20 | 1996-03-28 | Duke University | Oxidoreductase activity of manganic porphyrins |
CA2132690A1 (en) | 1994-09-22 | 1996-03-23 | Dean Willis | Control and modulation of inflammatory response in humans in need of such control and modulation |
US6013241A (en) | 1995-01-23 | 2000-01-11 | Schering Aktiengesellschaft | Use of porphyrin-complex or expanded porphyrin-complex compounds as an infarction localization diagnosticum |
ES2249784T3 (en) * | 1995-06-07 | 2006-04-01 | Duke University | ANTIOXIDANTGES OF OXIDANTS. |
WO1997006831A1 (en) | 1995-08-17 | 1997-02-27 | Monsanto Company | Methods of diagnostic image analysis using metal complexes of nitrogen-containing macrocyclic ligands |
US5648523A (en) | 1995-10-26 | 1997-07-15 | Chiang Long Y | Fullerene derivatives as free-radical scavengers |
US5948771A (en) | 1996-01-31 | 1999-09-07 | The Trustees Of Columbia University In The City Of New York | Method for treating heart failure using tetrapyrroles and metallotetrapyrroles |
US5756492A (en) | 1996-09-09 | 1998-05-26 | Sangstat Medical Corporation | Graft survival prolongation with porphyrins |
AU6650198A (en) * | 1997-02-05 | 1998-08-25 | Board Of Regents, The University Of Texas System | Porphyrin compounds as telomerase inhibitors |
US6214817B1 (en) | 1997-06-20 | 2001-04-10 | Monsanto Company | Substituted pyridino pentaazamacrocyle complexes having superoxide dismutase activity |
US6180620B1 (en) | 1997-06-20 | 2001-01-30 | G.D. Searle & Co. | Analgesic methods using synthetic catalysts for the dismutation of superoxide radicals |
JP2001521939A (en) * | 1997-11-03 | 2001-11-13 | デューク・ユニバーシティー | Substituted porphyrins |
EP1616869B1 (en) | 1999-01-25 | 2012-04-04 | National Jewish Health | Substituted porphyrins and their therapeutic use |
US6608050B2 (en) | 1999-07-20 | 2003-08-19 | Pharmacia & Upjohn Company | Lyophilizate of lipid complex of water insoluble porphyrins |
WO2001026655A1 (en) | 1999-10-13 | 2001-04-19 | Uab Research Foundation | Metalloporphyrin treatment of neurologic disease |
EP1148057A4 (en) | 1999-11-30 | 2002-05-29 | Photochemical Co Ltd | Nitroimidazole-supporting porphyrin complex |
JP3383839B2 (en) | 2000-03-13 | 2003-03-10 | 奈良先端科学技術大学院大学長 | Novel mercapto-substituted imidazolyl porphyrin metal complex monomer, polymer having the same as a repeating unit, and methods for producing them |
US6624187B1 (en) | 2000-06-12 | 2003-09-23 | Health Research, Inc. | Long wave length absorbing bacteriochlorin alkyl ether analogs |
US6403788B1 (en) | 2000-07-11 | 2002-06-11 | Eukarion, Inc. | Non-genotoxic metalloporphyrins as synthetic catalytic scavengers of reactive oxygen species |
US6573258B2 (en) * | 2000-09-27 | 2003-06-03 | Frontier Scientific, Inc. | Photodynamic porphyrin antimicrobial agents |
US6566517B2 (en) | 2001-06-06 | 2003-05-20 | Brookhaven Science Associates, Llc | Metalloporphyrins and their uses as imageable tumor-targeting agents for radiation therapy |
-
1998
- 1998-11-03 JP JP2000518967A patent/JP2001521939A/en active Pending
- 1998-11-03 CA CA2309154A patent/CA2309154C/en not_active Expired - Lifetime
- 1998-11-03 ES ES98956457T patent/ES2198767T3/en not_active Expired - Lifetime
- 1998-11-03 AT AT98956457T patent/ATE238307T1/en active
- 1998-11-03 DE DE69813898T patent/DE69813898T2/en not_active Expired - Lifetime
- 1998-11-03 IL IL13594998A patent/IL135949A0/en not_active IP Right Cessation
- 1998-11-03 WO PCT/US1998/023287 patent/WO1999023097A1/en active IP Right Grant
- 1998-11-03 AU AU12979/99A patent/AU737650B2/en not_active Expired
- 1998-11-03 EP EP98956457A patent/EP1045851B1/en not_active Expired - Lifetime
-
2001
- 2001-06-14 US US09/880,125 patent/US6916799B2/en not_active Expired - Lifetime
-
2005
- 2005-05-12 US US11/127,302 patent/US20060074062A1/en not_active Abandoned
-
2006
- 2006-09-15 US US11/532,408 patent/US20070179124A1/en not_active Abandoned
-
2008
- 2008-01-17 US US12/016,157 patent/US20080113956A1/en not_active Abandoned
-
2010
- 2010-06-09 JP JP2010131845A patent/JP5312404B2/en not_active Expired - Fee Related
Non-Patent Citations (2)
Title |
---|
Chemical Abstracts Service (C A S); 1 January 1990 (1990-01-01), MAKAKYAN V N, ET AL.: "NEW WATER-SOLUBLE METAL COMPLEXES OF MESO-TETRAKIS ¬3-N-(2'-HYDROXY ETHYL) PYRIDYL¾ PORPHYRINS AND THEIR PHARMACOLOGICAL ACTIVITY", XP002915848 * |
WHEELHOUSE R. T., ET AL.: "CATIONIC PORPHYRINS AS TELOMERASE INHIBITORS: THE INTERACTION OF TETRA-(N-METHYL-4-PYRIDYL)PORPHINE WITH QUADRUPLEX DNA.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, US, vol. 120., no. 13., 1 January 1998 (1998-01-01), US, pages 3261/3262., XP002069116, ISSN: 0002-7863, DOI: 10.1021/ja973792e * |
Cited By (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6583132B1 (en) | 1993-10-15 | 2003-06-24 | Duke University | Oxidant scavengers |
US6479477B1 (en) | 1998-04-24 | 2002-11-12 | Duke University | Substituted porphyrins |
WO2000019993A3 (en) * | 1998-10-06 | 2001-03-01 | Einstein Coll Med | Methods and compositions for decreasing mitochondrial overproduction of reactive oxygen species in cells |
WO2000019993A2 (en) * | 1998-10-06 | 2000-04-13 | Albert Einstein College Of Medicine Of Yeshiva University | Methods and compositions for decreasing mitochondrial overproduction of reactive oxygen species in cells |
US8217026B2 (en) | 1999-01-25 | 2012-07-10 | Aeolus Sciences, Inc. | Substituted porphyrins |
US9289434B2 (en) | 1999-01-25 | 2016-03-22 | Aeolus Sciences, Inc. | Substituted porphyrins |
US8470808B2 (en) | 1999-01-25 | 2013-06-25 | Jon D. Piganelli | Oxidant scavengers for treatment of type I diabetes or type II diabetes |
US8946202B2 (en) | 1999-01-25 | 2015-02-03 | Aeolus Sciences, Inc. | Substituted porphyrins |
EP1155019A1 (en) * | 1999-01-25 | 2001-11-21 | National Jewish Medical and Research Center | Substituted porphyrins |
KR100753184B1 (en) * | 1999-01-25 | 2007-08-30 | 내셔날 쥬이쉬 메디칼 앤드 리서치 센터 | Substituted Porphyrins |
US7189707B2 (en) | 1999-01-25 | 2007-03-13 | National Jewish Medical Research Center | Substituted porphyrins |
EP1616869A1 (en) * | 1999-01-25 | 2006-01-18 | National Jewish Medical and Research Center | Substituted porphyrins and their therapeutic use |
EP2295435A1 (en) * | 1999-01-25 | 2011-03-16 | National Jewish Health | Substituted porphyrins and their therapeutic use |
US7820644B2 (en) | 1999-01-25 | 2010-10-26 | Aelous Pharmaceuticals, Inc. | Substituted porphyrins |
US6544975B1 (en) | 1999-01-25 | 2003-04-08 | National Jewish Medical And Research Center | Substituted porphyrins |
US8546562B2 (en) | 1999-01-25 | 2013-10-01 | James D. Crapo | Substituted porphyrins |
EP1155019A4 (en) * | 1999-01-25 | 2002-07-03 | Nat Jewish Med & Res Center | Substituted porphyrins |
WO2001072327A3 (en) * | 2000-03-28 | 2002-03-28 | Univ Paris Descartes | Use of analogues of superoxide dismutase for treating hepatocellular insufficiencies |
WO2001072327A2 (en) * | 2000-03-28 | 2001-10-04 | Universite Rene Descartes (Paris V) | Use of analogues of superoxide dismutase for treating hepatocellular insufficiencies |
JP2009242421A (en) * | 2001-01-19 | 2009-10-22 | National Jewish Health | Cancer therapy |
EP1392328A4 (en) * | 2001-01-19 | 2004-11-17 | Nat Jewish Med & Res Center | Cancer therapy |
US8765729B2 (en) | 2001-01-19 | 2014-07-01 | Aeolus Sciences, Inc. | Cancer therapy |
EP1392328A2 (en) * | 2001-01-19 | 2004-03-03 | National Jewish Medical and Research Center | Cancer therapy |
EP1439842A1 (en) * | 2001-06-01 | 2004-07-28 | National Jewish Medical and Research Center | Oxidant scavengers for treatment of diabetes or use in transplantation or induction of immune tolerance |
EP1439842A4 (en) * | 2001-06-01 | 2009-09-02 | Nat Jewish Med & Res Center | Oxidant scavengers for treatment of diabetes or use in transplantation or induction of immune tolerance |
AU2002351267B2 (en) * | 2001-12-14 | 2007-04-05 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
US8003635B2 (en) | 2001-12-14 | 2011-08-23 | Novartis Ag | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
US7759333B2 (en) | 2001-12-14 | 2010-07-20 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
WO2003051458A1 (en) * | 2001-12-14 | 2003-06-26 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1513537A1 (en) * | 2002-06-07 | 2005-03-16 | Duke University | Substituted porphyrins |
EP1513537A4 (en) * | 2002-06-07 | 2006-09-06 | Univ Duke | Substituted porphyrins |
EP1567166A2 (en) * | 2002-12-06 | 2005-08-31 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1567166A4 (en) * | 2002-12-06 | 2009-06-17 | Alcon Inc | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
JP2006510668A (en) * | 2002-12-06 | 2006-03-30 | アルコン,インコーポレイテッド | Superoxide dismutase mimics for the treatment of eye disorders and diseases |
US8067405B2 (en) | 2002-12-06 | 2011-11-29 | Novartis Ag | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1581212A2 (en) * | 2002-12-06 | 2005-10-05 | Alcon, Inc. | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
EP1581212A4 (en) * | 2002-12-06 | 2008-11-05 | Alcon Inc | Superoxide dismutase mimics for the treatment of ocular disorders and diseases |
WO2004069273A1 (en) * | 2003-02-05 | 2004-08-19 | Photobiochem N.V. | Use of a porphyrin compound fro the treatment of skin fungi |
WO2005077269A1 (en) | 2004-02-09 | 2005-08-25 | Duke University | Substituted porphyrins |
WO2005097123A3 (en) * | 2004-03-29 | 2005-12-22 | Inotek Pharmaceuticals Corp | Pyridyl-substituted porphyrin compounds and methods of use thereof |
EA010834B1 (en) * | 2004-03-29 | 2008-12-30 | Инотек Фармасьютикалз Корпорейшн | Pyridyl-substituted porphyrin compounds and methods of use thereof |
US7432369B2 (en) | 2004-03-29 | 2008-10-07 | Inotek Pharmaceuticals Corporation | Pyridyl-substituted porphyrin compounds and methods of use thereof |
US8598150B1 (en) | 2008-04-02 | 2013-12-03 | Jonathan R. Brestoff | Composition and method for affecting obesity and related conditions |
US8809312B2 (en) | 2008-04-02 | 2014-08-19 | Jonathan R. Brestoff | Composition and method for affecting obesity and related conditions |
US8987245B2 (en) | 2008-04-02 | 2015-03-24 | Jonathan R. Brestoff Parker | Composition and method for affecting obesity and related conditions |
RU2506083C2 (en) * | 2008-05-23 | 2014-02-10 | Нэшнл Джуиш Хелт | Method of treating disorders associated with alkylating agent exposure |
US11382895B2 (en) | 2008-05-23 | 2022-07-12 | National Jewish Health | Methods for treating injury associated with exposure to an alkylating species |
EP2625180A4 (en) * | 2010-10-06 | 2014-05-14 | Aeolus Sciences Inc | Porphyrin treatment of neurodegenerative diseases |
EP2625180A2 (en) * | 2010-10-06 | 2013-08-14 | Aeolus Sciences, Inc. | Porphyrin treatment of neurodegenerative diseases |
Also Published As
Publication number | Publication date |
---|---|
CA2309154C (en) | 2010-02-16 |
US20020042407A1 (en) | 2002-04-11 |
US20070179124A1 (en) | 2007-08-02 |
US6916799B2 (en) | 2005-07-12 |
US20060074062A1 (en) | 2006-04-06 |
ES2198767T3 (en) | 2004-02-01 |
DE69813898D1 (en) | 2003-05-28 |
DE69813898T2 (en) | 2004-03-11 |
JP5312404B2 (en) | 2013-10-09 |
EP1045851A1 (en) | 2000-10-25 |
CA2309154A1 (en) | 1999-05-14 |
AU1297999A (en) | 1999-05-24 |
AU737650B2 (en) | 2001-08-23 |
JP2010229145A (en) | 2010-10-14 |
US20080113956A1 (en) | 2008-05-15 |
ATE238307T1 (en) | 2003-05-15 |
EP1045851B1 (en) | 2003-04-23 |
JP2001521939A (en) | 2001-11-13 |
IL135949A0 (en) | 2001-05-20 |
EP1045851A4 (en) | 2001-05-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1045851B1 (en) | Substituted porphyrins | |
EP1616869B1 (en) | Substituted porphyrins and their therapeutic use | |
CA2329751C (en) | Substituted porphyrins | |
US6046188A (en) | Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease | |
US6245758B1 (en) | Methods of use for peroxynitrite decomposition catalysts, pharmaceutical compositions therefor | |
US5834509A (en) | Synthetic catalytic free radical scavengers useful as antioxidants for prevention and therapy of disease | |
EP1320532A1 (en) | Tetrapyrroles | |
US20030069281A1 (en) | Tetrapyrroles |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 135949 Country of ref document: IL |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2309154 Country of ref document: CA Ref country code: CA Ref document number: 2309154 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12979/99 Country of ref document: AU |
|
NENP | Non-entry into the national phase |
Ref country code: KR |
|
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 2000 518967 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998956457 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1998956457 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 12979/99 Country of ref document: AU |
|
WWG | Wipo information: grant in national office |
Ref document number: 1998956457 Country of ref document: EP |