WO1999035293A3 - Solid phase selection of differentially expressed genes - Google Patents

Solid phase selection of differentially expressed genes Download PDF

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Publication number
WO1999035293A3
WO1999035293A3 PCT/US1999/000666 US9900666W WO9935293A3 WO 1999035293 A3 WO1999035293 A3 WO 1999035293A3 US 9900666 W US9900666 W US 9900666W WO 9935293 A3 WO9935293 A3 WO 9935293A3
Authority
WO
WIPO (PCT)
Prior art keywords
microparticles
solid phase
differentially expressed
sequencing
expressed genes
Prior art date
Application number
PCT/US1999/000666
Other languages
French (fr)
Other versions
WO1999035293A2 (en
Inventor
Glen Albrecht
Sydney Brenner
Robert Dubridge
Original Assignee
Lynx Therapeutics Inc
Glen Albrecht
Sydney Brenner
Robert Dubridge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc, Glen Albrecht, Sydney Brenner, Robert Dubridge filed Critical Lynx Therapeutics Inc
Priority to DE69930729T priority Critical patent/DE69930729D1/en
Priority to CA002317695A priority patent/CA2317695A1/en
Priority to EP99901448A priority patent/EP1054999B1/en
Priority to AU21139/99A priority patent/AU754929B2/en
Priority to HU0100242A priority patent/HUP0100242A3/en
Priority to JP2000527674A priority patent/JP4344086B2/en
Publication of WO1999035293A2 publication Critical patent/WO1999035293A2/en
Publication of WO1999035293A3 publication Critical patent/WO1999035293A3/en
Priority to NO20003531A priority patent/NO20003531L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Abstract

The invention provides a method and materials for monitoring and isolating differentially expressed genes. In accordance with the method of the invention, differently labeled populations of DNAs from sources to be compared are competitively hybridized with reference DNA cloned on solid phase supports, e.g. microparticles, to provide a differential expression library which, in the preferred embodiment, may be manipulated by fluorescence-activated cell sorting (FACS). Monitoring the relative signal intensity of the different fluorescent labels on the microparticles permits quantitative analysis of expression levels relative to the reference DNA. The invention also provides a method for identifying and isolating rare genes. Populations of microparticles having relative signal intensities of interest can be isolated by FACS and the attached DNAs identified by sequencing, such as with massively parallel signature sequencing (MPSS), or with conventional DNA sequencing protocols.
PCT/US1999/000666 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes WO1999035293A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE69930729T DE69930729D1 (en) 1998-01-09 1999-01-08 SELECTION OF DIFFERENTIALLY EXPRESSED GENES AT THE FESTPHASE
CA002317695A CA2317695A1 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes
EP99901448A EP1054999B1 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes
AU21139/99A AU754929B2 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes
HU0100242A HUP0100242A3 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes
JP2000527674A JP4344086B2 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes
NO20003531A NO20003531L (en) 1998-01-09 2000-07-07 Fixed phase selection of differentially expressed genes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US522298A 1998-01-09 1998-01-09
US09/005,222 1998-01-09
US09/130,446 1998-08-06
US09/130,546 US6265163B1 (en) 1998-01-09 1998-08-06 Solid phase selection of differentially expressed genes

Publications (2)

Publication Number Publication Date
WO1999035293A2 WO1999035293A2 (en) 1999-07-15
WO1999035293A3 true WO1999035293A3 (en) 1999-09-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/000666 WO1999035293A2 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes

Country Status (10)

Country Link
US (2) US6265163B1 (en)
EP (1) EP1054999B1 (en)
KR (1) KR100433782B1 (en)
AT (1) ATE322556T1 (en)
AU (1) AU754929B2 (en)
CA (1) CA2317695A1 (en)
DE (1) DE69930729D1 (en)
NO (1) NO20003531L (en)
PL (1) PL342114A1 (en)
WO (1) WO1999035293A2 (en)

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Also Published As

Publication number Publication date
NO20003531L (en) 2000-09-05
AU2113999A (en) 1999-07-26
EP1054999A2 (en) 2000-11-29
US6265163B1 (en) 2001-07-24
EP1054999B1 (en) 2006-04-05
PL342114A1 (en) 2001-05-21
AU754929B2 (en) 2002-11-28
ATE322556T1 (en) 2006-04-15
WO1999035293A2 (en) 1999-07-15
KR100433782B1 (en) 2004-06-04
KR20010033992A (en) 2001-04-25
DE69930729D1 (en) 2006-05-18
US6511802B1 (en) 2003-01-28
NO20003531D0 (en) 2000-07-07
CA2317695A1 (en) 1999-07-15

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