WO1999061052A3 - Oral immunization with papillomavirus virus-like particles - Google Patents

Oral immunization with papillomavirus virus-like particles Download PDF

Info

Publication number
WO1999061052A3
WO1999061052A3 PCT/US1999/011731 US9911731W WO9961052A3 WO 1999061052 A3 WO1999061052 A3 WO 1999061052A3 US 9911731 W US9911731 W US 9911731W WO 9961052 A3 WO9961052 A3 WO 9961052A3
Authority
WO
WIPO (PCT)
Prior art keywords
papillomavirus
hpv
type
virus
particles
Prior art date
Application number
PCT/US1999/011731
Other languages
French (fr)
Other versions
WO1999061052A2 (en
WO1999061052A9 (en
Inventor
Robert C Rose
Original Assignee
Univ Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rochester filed Critical Univ Rochester
Priority to CA 2329136 priority Critical patent/CA2329136A1/en
Priority to JP2000550511A priority patent/JP2002516291A/en
Priority to EP19990953290 priority patent/EP1079858A2/en
Priority to AU43162/99A priority patent/AU4316299A/en
Publication of WO1999061052A2 publication Critical patent/WO1999061052A2/en
Publication of WO1999061052A3 publication Critical patent/WO1999061052A3/en
Publication of WO1999061052A9 publication Critical patent/WO1999061052A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A61K2039/541—Mucosal route
    • A61K2039/542—Mucosal route oral/gastrointestinal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/788—Of specified organic or carbon-based composition
    • Y10S977/802—Virus-based particle
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/92—Detection of biochemical

Abstract

The present invention is directed to a method of expressing the papillomavirus capsid protein coding sequence in a cell using an expression system under conditions facilitating expression of the protein in the cell. In another aspect of the invention, it has been discovered that virus-like particle(s) (VLPs), fragment(s), capsomer(s) or portion(s) thereof are formed from the papillomavirus capsid protein. It was further discovered that the virus-like particle(s) comprises antigenic characteristics similar to those of native infectious papillomavirus particles. In one embodiment of the invention, there is provided a method of expressing the L1 major capsid protein of human papillomavirus type-6 (HPV-6) and type-11 (HPV-11) in Sf-9 insect cells using the baculovirus expression system, and the production of type 6 (HPV-6), type-11 (HPV-11), type-16 (HPV-16) and type-18 (HPV-18) virus-like particles. In yet another embodiment, the invention provides a method of vaccinating a mammal for papillomavirus by administering papillomavirus virus-like particles orally to a mammal in an amount sufficient to induce an immune response to the papillomavirus.
PCT/US1999/011731 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles WO1999061052A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA 2329136 CA2329136A1 (en) 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles
JP2000550511A JP2002516291A (en) 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles
EP19990953290 EP1079858A2 (en) 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles
AU43162/99A AU4316299A (en) 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/087,312 1998-05-29
US09/087,312 US6153201A (en) 1993-03-09 1998-05-29 Oral immunization with papillomavirus virus-like particles

Publications (3)

Publication Number Publication Date
WO1999061052A2 WO1999061052A2 (en) 1999-12-02
WO1999061052A3 true WO1999061052A3 (en) 2000-02-03
WO1999061052A9 WO1999061052A9 (en) 2000-06-15

Family

ID=22204420

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011731 WO1999061052A2 (en) 1998-05-29 1999-05-27 Oral immunization with papillomavirus virus-like particles

Country Status (6)

Country Link
US (1) US6153201A (en)
EP (1) EP1079858A2 (en)
JP (1) JP2002516291A (en)
AU (1) AU4316299A (en)
CA (1) CA2329136A1 (en)
WO (1) WO1999061052A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996011272A2 (en) * 1994-10-07 1996-04-18 Medigene Gesellschaft Für Molekularbiologische Diagnostik, Theraphie Und Technologie Mbh Papilloma virus-like particles, fusion proteins and process for producing the same
CU22871A1 (en) * 1998-12-02 2003-10-21 Ct Ingenieria Genetica Biotech FORMULATIONS CONTAINING PARTICULARS SIMILAR TO VIRUS AS IMMUNOPOTENCERS BY MUCOSAL ROUTE
AUPR011700A0 (en) * 2000-09-14 2000-10-05 Austin Research Institute, The Composition comprising immunogenic virus sized particles (VSP)
WO2003103570A2 (en) * 2001-08-01 2003-12-18 University Of Rochester Transcutaneous immunization against papillomavirus with papillomavirus virus-like particles
CU23031A1 (en) * 2002-01-24 2005-02-23 Ct Ingenieria Genetica Biotech SURFACE ANTIGEN OF THE HEPATITIS B VIRUS AS A MUCOSOPT IMMUNOPOTENTIATOR, RESULTS FORMULATIONS
US9045727B2 (en) * 2002-05-17 2015-06-02 Emory University Virus-like particles, methods of preparation, and immunogenic compositions
WO2004042001A2 (en) * 2002-05-17 2004-05-21 Emory University Virus-like particles, methods of preparation, and immonogenic compositions
US7763259B2 (en) * 2003-01-10 2010-07-27 The Regents Of The University Of Colorado Therapeutic and prophylactic vaccine for the treatment and prevention of papillomavirus infection
EP1790332B1 (en) 2004-09-10 2010-07-21 Asahi Glass Company, Limited Human papilloma virus vaccine for oral administration
US7691579B2 (en) 2005-04-15 2010-04-06 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen
US7445786B1 (en) 2006-04-10 2008-11-04 Manuela Rehtanz Diagnosing and protecting against tursiops truncatus papillomavirus
WO2008082719A2 (en) 2006-08-30 2008-07-10 University Of Rochester Combined human papillomavirus vlp/gene delivery system and use thereof as a vaccine for prophylaxis and immunotherapy of infectious diseases and tumors
MX2012011340A (en) * 2010-03-29 2012-11-16 Novartis Ag Composition comprising the amyloid beta 1-6 peptide coupled to a virus-like particle and an adjuvant.
AU2013209474B2 (en) * 2012-01-20 2017-11-16 South Dakota State University Inulin and inulin acetate formulations
EP4277654A1 (en) 2021-01-18 2023-11-22 Conserv Bioscience Limited Coronavirus immunogenic compositions, methods and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020137A1 (en) * 1993-03-09 1994-09-15 University Of Rochester Production of human papillomavirus capsid protein and virus-like particles
WO1995031532A1 (en) * 1994-05-16 1995-11-23 Merck & Co., Inc. Papillomavirus vaccines
WO1996015247A1 (en) * 1994-11-14 1996-05-23 Merck & Co., Inc. Purified papillomavirus proteins

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2170349B (en) * 1985-01-25 1989-01-11 Coal Ind A method of treating an instrument having an electrode
JP2648303B2 (en) * 1985-04-04 1997-08-27 ジョージタウン・ユニバーシティ Type-specific papillomavirus DNA sequences and peptides
US5071757A (en) * 1986-10-06 1991-12-10 Kreider John W Methods for propagating fastidious human viruses and for producing purified suspensions thereof
US5041385A (en) * 1986-11-01 1991-08-20 Oxford Gene Systems Limited Vector expressing fusion proteins and particles
US4870023A (en) * 1987-03-16 1989-09-26 American Biogenetic Sciences, Inc. Recombinant baculovirus occlusion bodies in vaccines and biological insecticides
US4956288A (en) * 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
US5045447A (en) * 1989-03-15 1991-09-03 Minson Anthony C Method of producing antibodies to HPV
NZ232913A (en) * 1989-03-15 1992-08-26 Gist Brocades Nv Il-3 produced recombinantly and purified to homogeneity; vectors and pharmaceutical preparations
WO1992010513A1 (en) * 1990-12-12 1992-06-25 The University Of Queensland Subunit papillomavirus vaccine and peptides for use therein
GB9105383D0 (en) * 1991-03-14 1991-05-01 Immunology Ltd An immunotherapeutic for cervical cancer
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
AU651727B2 (en) * 1991-07-19 1994-07-28 Csl Limited Papilloma virus vaccine
US5346811A (en) * 1991-07-22 1994-09-13 Cerveceria Polar Method and products for human papillomavirus detection
DE122007000098I1 (en) * 1992-06-25 2008-03-27 PAPILLOMA VIRUS VACCINE
US5437951A (en) * 1992-09-03 1995-08-01 The United States Of America As Represented By The Department Of Health And Human Services Self-assembling recombinant papillomavirus capsid proteins
WO1997002835A1 (en) * 1995-07-07 1997-01-30 Oravax, Inc. Intranasal vaccination against gastrointestinal disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020137A1 (en) * 1993-03-09 1994-09-15 University Of Rochester Production of human papillomavirus capsid protein and virus-like particles
WO1995031532A1 (en) * 1994-05-16 1995-11-23 Merck & Co., Inc. Papillomavirus vaccines
WO1996015247A1 (en) * 1994-11-14 1996-05-23 Merck & Co., Inc. Purified papillomavirus proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ROSE R C ET AL: "Oral vaccination of mice with human papillomavirus virus-like particles induces systemic virus-neutralizing antibodies", VACCINE,GB,BUTTERWORTH SCIENTIFIC. GUILDFORD, vol. 17, no. 17, 23 April 1999 (1999-04-23), pages 2129-2135, XP004164997, ISSN: 0264-410X *
SCHILLER JT ET AL: "Papillomavirus-like particles and HPV vaccine development", SEMINARS IN CANCER BIOLOGY, vol. 7, 1996, pages 373 - 382, XP000857170 *

Also Published As

Publication number Publication date
AU4316299A (en) 1999-12-13
JP2002516291A (en) 2002-06-04
WO1999061052A2 (en) 1999-12-02
US6153201A (en) 2000-11-28
CA2329136A1 (en) 1999-12-02
EP1079858A2 (en) 2001-03-07
WO1999061052A9 (en) 2000-06-15

Similar Documents

Publication Publication Date Title
WO1999061052A3 (en) Oral immunization with papillomavirus virus-like particles
Christensen et al. Immunization with viruslike particles induces long-term protection of rabbits against challenge with cottontail rabbit papillomavirus
Pastrana et al. Cross-neutralization of cutaneous and mucosal Papillomavirus types with anti-sera to the amino terminus of L2
DE69835294T2 (en) METHOD FOR THE VITRO DISPOSAL AND RE-COMPOSITION OF PAPILLOMA VIRUS LIKE PARTICLES (VLPS)
FR11C0022I2 (en) HUMAN PAPILLOMA VIRUS CAPSID PROTEIN PRODUCTION AND VIRAL-LIKE PARTICLES
NO2018010I1 (en) HPV 31 L1 protein
JP2005270111A (en) Papilloma virus-like particles, fusion proteins and methods for their production
NL300313I1 (en) Human papillomavirus vaccine
JP2010263899A (en) Chimeric human papillomavirus 16l1 protein including l2 peptide, virus-like particle prepared therefrom, and method for preparing the particle
RU2007113184A (en) ORAL VACCINE
SK4222002A3 (en) Vaccine
CA2340422A1 (en) Method for producing yeast expressed hpv types 6 and 16 capsid proteins
Frazer HPV vaccines and the prevention of cervical cancer
CN101440371A (en) 16 type human papilloma virus major capsid protein L1 gene
CN114127092B (en) Human papillomavirus multivalent immunogenic compositions
De Carvalho Cervical cancer vaccination: the real meaning of cross-protection and its impact on the prevention
Inglis HPV Vaccine

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: C2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/18-18/18, DRAWINGS, REPLACED BY NEW PAGES 1/18-18/18

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 550511

Kind code of ref document: A

Format of ref document f/p: F

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1999953290

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999953290

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

ENP Entry into the national phase

Ref document number: 2329136

Country of ref document: CA