WO1999063112A3 - Pcr techniques for detecting microbial and viral contaminants in foodstuffs - Google Patents

Pcr techniques for detecting microbial and viral contaminants in foodstuffs Download PDF

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Publication number
WO1999063112A3
WO1999063112A3 PCT/US1999/010940 US9910940W WO9963112A3 WO 1999063112 A3 WO1999063112 A3 WO 1999063112A3 US 9910940 W US9910940 W US 9910940W WO 9963112 A3 WO9963112 A3 WO 9963112A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
dna
universal
acid sequence
foodstuffs
Prior art date
Application number
PCT/US1999/010940
Other languages
French (fr)
Other versions
WO1999063112A8 (en
WO1999063112A2 (en
Inventor
Thomas L Romick
Mark S Fraser
Original Assignee
Hunt Wesson Inc
Thomas L Romick
Mark S Fraser
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunt Wesson Inc, Thomas L Romick, Mark S Fraser filed Critical Hunt Wesson Inc
Priority to AU40012/99A priority Critical patent/AU4001299A/en
Priority to EP99923185A priority patent/EP1080224A2/en
Publication of WO1999063112A2 publication Critical patent/WO1999063112A2/en
Publication of WO1999063112A3 publication Critical patent/WO1999063112A3/en
Publication of WO1999063112A8 publication Critical patent/WO1999063112A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

A method of detecting the presence of living or dead microorganisms and viruses in a sample comprises adding to a predetermined volume of a sample comprising nucleic acid-containing microbe(s) and/or virus(es), known amounts of a pair of primers binding to sequences upstream and downstream to a universal or specific microbial and/or viral nucleic acid sequence and polymerase chain reaction (PCR) reagents, cycling the mixture to amplify the universal or specific microbial and/or viral nucleic acid sequence; adding a polynucleotide comprising a DNA internal segment that is hybridizably complementary to at least a portion of the universal or specific nucleic acid sequence; and a first and second DNA arm segment adjoining the DNA internal segment, the first DNA arm segment ending in a 5' terminus and the second DNA arm segment ending in a 3' terminus, the arm segments comprising nucleotide sequences such that they are hybridizably complementary to one another. Also disclosed are polynucleotides and kits useful for practicing the method.
PCT/US1999/010940 1998-05-18 1999-05-18 Pcr techniques for detecting microbial and viral contaminants in foodstuffs WO1999063112A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU40012/99A AU4001299A (en) 1998-05-18 1999-05-18 Pcr techniques for detecting microbial and viral contaminants in foodstuffs
EP99923185A EP1080224A2 (en) 1998-05-18 1999-05-18 Pcr techniques for detecting microbial and viral contaminants in foodstuffs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8602598P 1998-05-18 1998-05-18
US60/086,025 1998-05-18

Publications (3)

Publication Number Publication Date
WO1999063112A2 WO1999063112A2 (en) 1999-12-09
WO1999063112A3 true WO1999063112A3 (en) 2000-03-16
WO1999063112A8 WO1999063112A8 (en) 2000-04-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010940 WO1999063112A2 (en) 1998-05-18 1999-05-18 Pcr techniques for detecting microbial and viral contaminants in foodstuffs

Country Status (4)

Country Link
US (1) US6468743B1 (en)
EP (1) EP1080224A2 (en)
AU (1) AU4001299A (en)
WO (1) WO1999063112A2 (en)

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Also Published As

Publication number Publication date
EP1080224A2 (en) 2001-03-07
WO1999063112A8 (en) 2000-04-27
AU4001299A (en) 1999-12-20
WO1999063112A2 (en) 1999-12-09
US6468743B1 (en) 2002-10-22

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