WO2000050644A3 - Methods for purifying dna using immobilized capture probes - Google Patents

Methods for purifying dna using immobilized capture probes Download PDF

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Publication number
WO2000050644A3
WO2000050644A3 PCT/US2000/004939 US0004939W WO0050644A3 WO 2000050644 A3 WO2000050644 A3 WO 2000050644A3 US 0004939 W US0004939 W US 0004939W WO 0050644 A3 WO0050644 A3 WO 0050644A3
Authority
WO
WIPO (PCT)
Prior art keywords
capture probes
methods
immobilized capture
purifying dna
dna
Prior art date
Application number
PCT/US2000/004939
Other languages
French (fr)
Other versions
WO2000050644A2 (en
Inventor
Christopher P Adams
T Christian Boles
Lawrence Weir
Rahul K Dhanda
Nevin M Summers
Original Assignee
Mosaic Technologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies filed Critical Mosaic Technologies
Priority to EP00915885A priority Critical patent/EP1163371A2/en
Priority to AU37083/00A priority patent/AU3708300A/en
Priority to CA002369002A priority patent/CA2369002A1/en
Priority to JP2000601207A priority patent/JP2002537778A/en
Publication of WO2000050644A2 publication Critical patent/WO2000050644A2/en
Publication of WO2000050644A3 publication Critical patent/WO2000050644A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase

Abstract

Methods employing purification devices comprising an electrophoretic medium containing immobilized capture probes for the purification of DNA are disclosed.
PCT/US2000/004939 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes WO2000050644A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00915885A EP1163371A2 (en) 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes
AU37083/00A AU3708300A (en) 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes
CA002369002A CA2369002A1 (en) 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes
JP2000601207A JP2002537778A (en) 1999-02-26 2000-02-25 Method for purifying DNA using immobilized capture probe

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25946799A 1999-02-26 1999-02-26
US09/259,467 1999-02-26

Publications (2)

Publication Number Publication Date
WO2000050644A2 WO2000050644A2 (en) 2000-08-31
WO2000050644A3 true WO2000050644A3 (en) 2001-04-05

Family

ID=22985081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004939 WO2000050644A2 (en) 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes

Country Status (5)

Country Link
EP (1) EP1163371A2 (en)
JP (1) JP2002537778A (en)
AU (1) AU3708300A (en)
CA (1) CA2369002A1 (en)
WO (1) WO2000050644A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0981647B1 (en) 1997-05-16 2006-08-09 EXACT Sciences Corporation Electrophoretic analysis of molecules using immobilized probes
WO1999022228A1 (en) * 1997-10-24 1999-05-06 Northeastern University A multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis
WO2003034026A2 (en) 2001-10-15 2003-04-24 Biocept, Inc. Microwell biochip
JP2005110503A (en) * 2003-10-02 2005-04-28 Arkray Inc Method for purifying nucleic acid and device therefor
WO2005047881A2 (en) 2003-11-05 2005-05-26 Exact Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020831A1 (en) * 1993-03-08 1994-09-15 Norman Wainwright Aligned fiber diagnostic chromatography
US5556598A (en) * 1992-09-18 1996-09-17 Amersham International Plc. Device and method for affinity separation
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5556598A (en) * 1992-09-18 1996-09-17 Amersham International Plc. Device and method for affinity separation
WO1994020831A1 (en) * 1993-03-08 1994-09-15 Norman Wainwright Aligned fiber diagnostic chromatography
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method

Also Published As

Publication number Publication date
AU3708300A (en) 2000-09-14
WO2000050644A2 (en) 2000-08-31
EP1163371A2 (en) 2001-12-19
CA2369002A1 (en) 2000-08-31
JP2002537778A (en) 2002-11-12

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