WO2000055363A3 - Analysis of differential gene expression - Google Patents

Analysis of differential gene expression Download PDF

Info

Publication number
WO2000055363A3
WO2000055363A3 PCT/GB2000/000807 GB0000807W WO0055363A3 WO 2000055363 A3 WO2000055363 A3 WO 2000055363A3 GB 0000807 W GB0000807 W GB 0000807W WO 0055363 A3 WO0055363 A3 WO 0055363A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
beads
target
mixture
gene expression
Prior art date
Application number
PCT/GB2000/000807
Other languages
French (fr)
Other versions
WO2000055363A2 (en
Inventor
Nicholas Thomas
Alan Waggoner
Original Assignee
Amersham Pharm Biotech Uk Ltd
Amersham Pharm Biotech Inc
Nicholas Thomas
Alan Waggoner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharm Biotech Uk Ltd, Amersham Pharm Biotech Inc, Nicholas Thomas, Alan Waggoner filed Critical Amersham Pharm Biotech Uk Ltd
Priority to EP00907817A priority Critical patent/EP1163367A2/en
Priority to AU29284/00A priority patent/AU2928400A/en
Priority to JP2000605779A priority patent/JP2002538836A/en
Publication of WO2000055363A2 publication Critical patent/WO2000055363A2/en
Publication of WO2000055363A3 publication Critical patent/WO2000055363A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00664—Three-dimensional arrays

Abstract

The invention provides methods for detecting the differential expression or presence of two analytes, and more specifically to procedures which provide for rapid and efficient analysis of gene expression in biological systems. In particular, the invention provides a method of detecting and analysing differences between nucleic acids from two sources, which method comprises: a. providing nucleic acids from two sources as labelled probes; b. forming a mixture of the labelled probes with pooled reagents wherein each reagent is a population of beads carrying a polynucleotide target, the target of one reagent being different from the target of another reagent, the beads of one reagent being distinguishable from the beads of another reagent; c. incubating the mixture under conditions to promote specific hybridisation between probes and targets; and, d. analysing beads in the mixture by flow cytometry.
PCT/GB2000/000807 1999-03-12 2000-03-09 Analysis of differential gene expression WO2000055363A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00907817A EP1163367A2 (en) 1999-03-12 2000-03-09 Analysis of differential gene expression
AU29284/00A AU2928400A (en) 1999-03-12 2000-03-09 Analysis of differential gene expression
JP2000605779A JP2002538836A (en) 1999-03-12 2000-03-09 Analysis of changes in gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9905807.5A GB9905807D0 (en) 1999-03-12 1999-03-12 Analysis of differential gene expression
GB9905807.5 1999-03-12

Publications (2)

Publication Number Publication Date
WO2000055363A2 WO2000055363A2 (en) 2000-09-21
WO2000055363A3 true WO2000055363A3 (en) 2000-12-21

Family

ID=10849574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/000807 WO2000055363A2 (en) 1999-03-12 2000-03-09 Analysis of differential gene expression

Country Status (5)

Country Link
EP (1) EP1163367A2 (en)
JP (1) JP2002538836A (en)
AU (1) AU2928400A (en)
GB (1) GB9905807D0 (en)
WO (1) WO2000055363A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7875600A (en) * 1999-10-08 2001-04-23 University Of Utah Research Foundation Particle analysis assay for biomolecular quantification
US6994971B1 (en) 1999-10-08 2006-02-07 University Of Utah Research Foundation Particle analysis assay for biomolecular quantification
DK1360491T3 (en) * 2001-02-13 2007-08-20 Pronostics Ltd Biochemical method and apparatus for detecting protein properties
ES2284833T3 (en) * 2001-02-13 2007-11-16 Pronostics Limited BIOCHEMICAL PROCEDURE AND APPLIANCE TO DETECT GENETIC CHARACTERISTICS.
WO2003045310A2 (en) 2001-11-21 2003-06-05 Applera Corporation Digital assay
DE10323901A1 (en) * 2003-05-26 2005-01-05 Institut Virion/Serion Gmbh Method and test means for the examination and / or detection of biomolecules and / or active substances in liquid samples
WO2005071412A2 (en) 2004-01-09 2005-08-04 Applera Corporation Phosphor particle coded beads
CN101460619B (en) 2006-06-06 2012-07-25 松下电器产业株式会社 Method of modifying nucleotide chain
WO2014141386A1 (en) * 2013-03-12 2014-09-18 株式会社日立製作所 Two-dimensional cell array device and apparatus for gene quantification and sequence analysis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012014A1 (en) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Molecular tagging system
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
WO1998026098A1 (en) * 1996-12-13 1998-06-18 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
WO1999019515A1 (en) * 1997-10-14 1999-04-22 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
WO1999035293A2 (en) * 1998-01-09 1999-07-15 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
WO1999064867A1 (en) * 1997-12-04 1999-12-16 Amersham Pharmacia Biotech Uk Limited Multiple assay method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996012014A1 (en) * 1994-10-13 1996-04-25 Lynx Therapeutics, Inc. Molecular tagging system
WO1997014028A2 (en) * 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
WO1998026098A1 (en) * 1996-12-13 1998-06-18 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
WO1999019515A1 (en) * 1997-10-14 1999-04-22 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
WO1999064867A1 (en) * 1997-12-04 1999-12-16 Amersham Pharmacia Biotech Uk Limited Multiple assay method
WO1999035293A2 (en) * 1998-01-09 1999-07-15 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE ONLINE October 1998 (1998-10-01), "LUMINEX AWARDED GRANT TO DEVELOP DNA TECHNOLOGY", XP002145219 *
LACKNER ET AL.: "MULTIPLEX DNA-UND RNA-ANALYSE AN FLUORESZENTEN MICROBEADS ALS ALTERNATIVE ZUM DNA-ARRAY", MEDIZINISCHE GENETIK, vol. 11, March 1999 (1999-03-01), pages 16 - 17, XP000930079 *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY", SCIENCE, vol. 270, no. 5235, 20 October 1995 (1995-10-20), pages 467 - 470, XP000644675, ISSN: 0036-8075 *
VAN NESS J ET AL: "A VERSATILE SOLID SUPPORT SYSTEM FOR OLIGODEOXYNUCLEOTIDE PROBE -BASED HYBRIDIZATION ASSAYS", NUCLEIC ACIDS RESEARCH, vol. 19, no. 12, 25 June 1991 (1991-06-25), pages 3345 - 3350, XP000208399, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2928400A (en) 2000-10-04
JP2002538836A (en) 2002-11-19
WO2000055363A2 (en) 2000-09-21
EP1163367A2 (en) 2001-12-19
GB9905807D0 (en) 1999-05-05

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