WO2000058481A3 - Phosphatases with improved phytase activity - Google Patents

Phosphatases with improved phytase activity Download PDF

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Publication number
WO2000058481A3
WO2000058481A3 PCT/US2000/008590 US0008590W WO0058481A3 WO 2000058481 A3 WO2000058481 A3 WO 2000058481A3 US 0008590 W US0008590 W US 0008590W WO 0058481 A3 WO0058481 A3 WO 0058481A3
Authority
WO
WIPO (PCT)
Prior art keywords
phytase activity
phosphatase
phosphatases
improved
improved phytase
Prior art date
Application number
PCT/US2000/008590
Other languages
French (fr)
Other versions
WO2000058481A9 (en
WO2000058481A2 (en
Inventor
Xingen Lei
Original Assignee
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc filed Critical Cornell Res Foundation Inc
Priority to CA002365418A priority Critical patent/CA2365418C/en
Priority to AU40567/00A priority patent/AU4056700A/en
Priority to DE60021715T priority patent/DE60021715T2/en
Priority to BRPI0009516-8B1A priority patent/BR0009516B1/en
Priority to MXPA01009809A priority patent/MXPA01009809A/en
Priority to AT00919962T priority patent/ATE301198T1/en
Priority to EP00919962A priority patent/EP1165806B1/en
Publication of WO2000058481A2 publication Critical patent/WO2000058481A2/en
Publication of WO2000058481A3 publication Critical patent/WO2000058481A3/en
Publication of WO2000058481A9 publication Critical patent/WO2000058481A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines

Abstract

The present invention provides phosphatases with improved phytase activity. The invention provides proteolytic fragments of phosphatase having improved phytase activity. A recombinant gene encoding a phosphatase fragment having improved phytase activity is also provided. The invention also includes a method of increasing the phytase activity of phosphatase by treating the phosphatase with a protease. In addition, the invention provides a phosphatase, AppA2, having improved properties.
PCT/US2000/008590 1999-03-31 2000-03-31 Phosphatases with improved phytase activity WO2000058481A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002365418A CA2365418C (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity
AU40567/00A AU4056700A (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity
DE60021715T DE60021715T2 (en) 1999-03-31 2000-03-31 PHOSPAHATASES WITH IMPROVED PHYTASE ACTIVITY
BRPI0009516-8B1A BR0009516B1 (en) 1999-03-31 2000-03-31 recombinant vector and microbial host cell.
MXPA01009809A MXPA01009809A (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity.
AT00919962T ATE301198T1 (en) 1999-03-31 2000-03-31 PHOSPAHATASES WITH IMPROVED PHYTASE ACTIVITY
EP00919962A EP1165806B1 (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12703299P 1999-03-31 1999-03-31
US60/127,032 1999-03-31

Publications (3)

Publication Number Publication Date
WO2000058481A2 WO2000058481A2 (en) 2000-10-05
WO2000058481A3 true WO2000058481A3 (en) 2001-01-04
WO2000058481A9 WO2000058481A9 (en) 2002-08-29

Family

ID=22427982

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/008590 WO2000058481A2 (en) 1999-03-31 2000-03-31 Phosphatases with improved phytase activity

Country Status (12)

Country Link
US (2) US6511699B1 (en)
EP (1) EP1165806B1 (en)
CN (1) CN100347303C (en)
AT (1) ATE301198T1 (en)
AU (1) AU4056700A (en)
BR (1) BR0009516B1 (en)
CA (1) CA2365418C (en)
DE (1) DE60021715T2 (en)
DK (1) DK1165806T3 (en)
ES (1) ES2248067T3 (en)
MX (1) MXPA01009809A (en)
WO (1) WO2000058481A2 (en)

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Also Published As

Publication number Publication date
EP1165806A2 (en) 2002-01-02
EP1165806B1 (en) 2005-08-03
CN1413253A (en) 2003-04-23
AU4056700A (en) 2000-10-16
BR0009516A (en) 2001-12-26
MXPA01009809A (en) 2002-07-30
WO2000058481A9 (en) 2002-08-29
WO2000058481A2 (en) 2000-10-05
ES2248067T3 (en) 2006-03-16
DE60021715D1 (en) 2005-09-08
BR0009516B1 (en) 2013-09-10
CN100347303C (en) 2007-11-07
US6511699B1 (en) 2003-01-28
ATE301198T1 (en) 2005-08-15
CA2365418C (en) 2009-05-26
CA2365418A1 (en) 2000-10-05
DE60021715T2 (en) 2006-06-01
DK1165806T3 (en) 2005-12-05
US20030072844A1 (en) 2003-04-17
US6974690B2 (en) 2005-12-13

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