WO2000061720A3 - Allele-specific strand displacement amplification primers and method - Google Patents

Allele-specific strand displacement amplification primers and method Download PDF

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Publication number
WO2000061720A3
WO2000061720A3 PCT/US2000/009862 US0009862W WO0061720A3 WO 2000061720 A3 WO2000061720 A3 WO 2000061720A3 US 0009862 W US0009862 W US 0009862W WO 0061720 A3 WO0061720 A3 WO 0061720A3
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WIPO (PCT)
Prior art keywords
allele
specific
strand displacement
displacement amplification
sense primer
Prior art date
Application number
PCT/US2000/009862
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French (fr)
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WO2000061720A9 (en
WO2000061720A2 (en
Inventor
Michael I Nerenberg
Carl F Edman
Preshant P Metha
Original Assignee
Nanogen Becton Dickinson Partn
Michael I Nerenberg
Carl F Edman
Preshant P Metha
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Application filed by Nanogen Becton Dickinson Partn, Michael I Nerenberg, Carl F Edman, Preshant P Metha filed Critical Nanogen Becton Dickinson Partn
Publication of WO2000061720A2 publication Critical patent/WO2000061720A2/en
Publication of WO2000061720A3 publication Critical patent/WO2000061720A3/en
Publication of WO2000061720A9 publication Critical patent/WO2000061720A9/en

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Abstract

Disclosed and described is a method for allele-specific strand displacement amplification of target nucleic acids. The primer set used to perform allele-specific strand displacement amplification preferably comprises a sense primer and a complementary anti-sense primer wherein one end of the anti-sense primer preferably has a sequence complementary to the sense sequence of a target nucleic acid sequence containing a specific allele or nucleic acid base. The specific allele may include a base that is considered 'normal' sequence or it may include a point mutation. The sense primer may incorporate a biotin moiety at its 5' end to facilitate in one embodiment the capture of amplicons to specific sites on a bioelectronic microarray.
PCT/US2000/009862 1999-04-12 2000-04-11 Allele-specific strand displacement amplification primers and method WO2000061720A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/290,577 US6238868B1 (en) 1999-04-12 1999-04-12 Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US09/290,577 1999-04-12

Publications (3)

Publication Number Publication Date
WO2000061720A2 WO2000061720A2 (en) 2000-10-19
WO2000061720A3 true WO2000061720A3 (en) 2001-01-18
WO2000061720A9 WO2000061720A9 (en) 2002-08-29

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PCT/US2000/009843 WO2000061818A1 (en) 1999-04-12 2000-04-11 Multiplex amplification and separation of nucleic acid sequences using ligation-dependent strand displacement amplification and bioelectronic chip technology
PCT/US2000/009862 WO2000061720A2 (en) 1999-04-12 2000-04-11 Allele-specific strand displacement amplification primers and method

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PCT/US2000/009843 WO2000061818A1 (en) 1999-04-12 2000-04-11 Multiplex amplification and separation of nucleic acid sequences using ligation-dependent strand displacement amplification and bioelectronic chip technology

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WO (2) WO2000061818A1 (en)

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