WO2000063701A3 - Microarrays of polypeptides - Google Patents

Microarrays of polypeptides Download PDF

Info

Publication number
WO2000063701A3
WO2000063701A3 PCT/US2000/010171 US0010171W WO0063701A3 WO 2000063701 A3 WO2000063701 A3 WO 2000063701A3 US 0010171 W US0010171 W US 0010171W WO 0063701 A3 WO0063701 A3 WO 0063701A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
microarrays
ligands
quantitation
antigens
Prior art date
Application number
PCT/US2000/010171
Other languages
French (fr)
Other versions
WO2000063701A9 (en
WO2000063701A2 (en
Inventor
Patrick Brown
Brian Haab
Original Assignee
Univ Leland Stanford Junior
Patrick Brown
Brian Haab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior, Patrick Brown, Brian Haab filed Critical Univ Leland Stanford Junior
Priority to JP2000612755A priority Critical patent/JP2002542487A/en
Priority to EP00923395A priority patent/EP1169649A2/en
Priority to CA002366123A priority patent/CA2366123A1/en
Publication of WO2000063701A2 publication Critical patent/WO2000063701A2/en
Publication of WO2000063701A3 publication Critical patent/WO2000063701A3/en
Publication of WO2000063701A9 publication Critical patent/WO2000063701A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Microarrays of polypeptides on a solid support are provided. The microarray compositions find use in the multiplexed detection and quantitation of ligands, e.g. antigens or antibodies, in a miniaturized format. The substrate is used for detecting binding of ligands to a plurality of polypeptides for screening and diagnostic purposes.
PCT/US2000/010171 1999-04-15 2000-04-14 Microarrays of polypeptides WO2000063701A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000612755A JP2002542487A (en) 1999-04-15 2000-04-14 Polypeptide microarray
EP00923395A EP1169649A2 (en) 1999-04-15 2000-04-14 Microarrays of polypeptides
CA002366123A CA2366123A1 (en) 1999-04-15 2000-04-14 Microarrays of polypeptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12944999P 1999-04-15 1999-04-15
US60/129,449 1999-04-15

Publications (3)

Publication Number Publication Date
WO2000063701A2 WO2000063701A2 (en) 2000-10-26
WO2000063701A3 true WO2000063701A3 (en) 2001-03-08
WO2000063701A9 WO2000063701A9 (en) 2002-06-27

Family

ID=22440002

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/010171 WO2000063701A2 (en) 1999-04-15 2000-04-14 Microarrays of polypeptides

Country Status (4)

Country Link
EP (1) EP1169649A2 (en)
JP (1) JP2002542487A (en)
CA (1) CA2366123A1 (en)
WO (1) WO2000063701A2 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
AU2001292959A1 (en) 2000-09-22 2002-04-02 Clontech Laboratories, Inc. Highly sensitive proteomic analysis methods and kits and systems for practicing the same
ATE349011T1 (en) 2000-10-03 2007-01-15 Minerva Biotechnologies Corp MAGNETIC IN SITU DILUTION PROCESS
EP1336104B1 (en) * 2000-11-01 2009-03-25 Minerva Biotechnologies Corporation Proteomics based drug comparison methods
EP1377827A2 (en) * 2001-04-10 2004-01-07 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts Method for determining analyte molecules in a sample
JP3893030B2 (en) * 2001-04-18 2007-03-14 独立行政法人科学技術振興機構 Bacterial endotoxin adsorbent and screening method thereof
US20050009204A1 (en) * 2003-07-11 2005-01-13 Ye Fang Multiplexed binding assays for receptor arrays
GB0113358D0 (en) * 2001-06-01 2001-07-25 Scient Generics Ltd Enhanced target capture
US7902121B2 (en) 2001-07-02 2011-03-08 The Board Of Trustees Of The Leland Stanford Junior University MHC-antigen arrays for detection and characterization of immune responses
JP2005514617A (en) * 2001-07-02 2005-05-19 ザ、ボード、オブ、トラスティーズ、オブ、ザ、リーランド、スタンフォード、ジュニア、ユニバーシティ Microarray for cell phenotyping and manipulation
WO2003018844A1 (en) * 2001-08-21 2003-03-06 Mcw Research Foundation, Inc. Method and apparatus for three label microarrays
JP2003149242A (en) * 2001-11-09 2003-05-21 Gifu Univ Method of detecting antibody and antigen micro-array
WO2003062831A1 (en) * 2002-01-24 2003-07-31 The Regents Of The University Of California Method for determining differences in molecular interactions and for screening a combinatorial library
WO2003069345A2 (en) * 2002-02-12 2003-08-21 Biotesys Gmbh Analysis of a protein expression pattern
FR2840409B1 (en) * 2002-05-28 2004-08-27 Dev Des Antigenes Combinatoire DEVICE FOR PRESENTING PEPTIDES OR PROTEINS, ITS PREPARATION METHOD AND USES THEREOF
EP1527339B1 (en) 2002-06-03 2006-08-16 PamGene B.V. Method for high throughput cell-based assays using versatile living microarrays
US20040142379A1 (en) * 2003-01-16 2004-07-22 Carlsberg Research Laboratory Affinity fishing for ligands and proteins receptors
US7029855B1 (en) * 2003-01-28 2006-04-18 Proteomyx Inc. Radioactive multiplexing analytical methods for biomarkers discovery
US20040209262A1 (en) * 2003-04-21 2004-10-21 Bass Jay K. Biopolymeric arrays comprising test probes for two or more different species and methods for using the same
EP1689882B1 (en) * 2003-10-07 2014-11-26 TRAN, Nathaniel Tue Radioactive multiplexing analytical methods
US20100003189A1 (en) 2006-07-14 2010-01-07 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
WO2009075773A2 (en) 2007-12-07 2009-06-18 Goldstein Steven A Identification of toxin ligands
EP3508854A1 (en) 2010-04-27 2019-07-10 The Regents of The University of California Cancer biomarkers and methods of use thereof
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
EP3591401A1 (en) 2018-07-06 2020-01-08 Euroimmun Medizinische Labordiagnostika AG Method for the automated detection of antibodies in a liquid biological sample using an antigen chip
EP3591406A1 (en) 2018-07-06 2020-01-08 Euroimmun Medizinische Labordiagnostika AG Device and method for antibody detection
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
CN112557644B (en) * 2020-12-21 2024-03-22 珠海碳云智能科技有限公司 Screening method for polypeptide for detecting target antibody and application of screened polypeptide

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324633A (en) * 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
WO1997042507A1 (en) * 1996-05-02 1997-11-13 Isis Innovation Limited Peptide array and method
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2283131T3 (en) * 1998-08-28 2007-10-16 Poly-An Gesellschaft Zur Herstellung Von Polymeren Fur Spezielle Anwendungen Und Analytik Mbh PROCEDURE FOR THE MANUFACTURE OF POLYMER SUPPORTS IN SOLID PHASE.
FR2789401B1 (en) * 1999-02-08 2003-04-04 Cis Bio Int METHOD FOR MANUFACTURING LIGAND DIES ADDRESSED ON A MEDIUM

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5324633A (en) * 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
WO1997042507A1 (en) * 1996-05-02 1997-11-13 Isis Innovation Limited Peptide array and method

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHRISTOPHER P. HOLMES ET AL.: "THE USE OF LIGHT-DIRECTED COMBINATORIAL PEPTIDE SYNTHESIS IN EPITOPE MAPPING", BIOPOLYMERS (PEPTIDE SCIENCE), vol. 37, no. 3, 1995, NEW YORK, US, pages 199 - 211, XP000654595, ISSN: 0006-3525 *
JEFFREY W. JACOBS & STEPHEN P.A. FODOR: "COMBINATORIAL CHEMISTRY - APPLICATIONS OF LIGHT DIRECTED CHEMICAL SYNTHESIS", TIBTECH, vol. 12, no. 1, 1994, CAMBRIDGE, UK, pages 19 - 26, XP000652268 *
RONALD FRANK: "SPOT-SYNTHESIS: AN EASY TECHNIQUE FOR THE POSITIONALLY ADDRESSABLE, PARALLEL CHEMICAL SYNTHESIS ON A MEMBRANE SUPPORT", TETRAHEDRON, vol. 48, no. 42, 16 October 1992 (1992-10-16), UK, pages 9217 - 9232, XP000353580, ISSN: 0040-4020 *
S. M. RUBER ET AL.: "LIGHT-DIRECTED COMBINATORIAL PEPTIDE SYNTHESIS", 1992, PROCEEDINGS OF THE 12TH AMERICAN PEPTIDE SYMPOSIUM, 16-21 JUNE 1991, CAMBRIDGE, MA, USA, LEIDEN, NL, XP000371924, 185690 *
See also references of EP1169649A2 *
STEPHEN P.A. FODOR ET AL.: "LIGHT-DIRECTED, SPATIALLY ADDRESSABLE PARALLEL CHEMICAL SYNTHESIS", SCIENCE, vol. 251, no. 4995, 15 February 1991 (1991-02-15), US, pages 767 - 773, XP000486899, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2000063701A9 (en) 2002-06-27
CA2366123A1 (en) 2000-10-26
EP1169649A2 (en) 2002-01-09
WO2000063701A2 (en) 2000-10-26
JP2002542487A (en) 2002-12-10

Similar Documents

Publication Publication Date Title
WO2000063701A3 (en) Microarrays of polypeptides
EP1975620A3 (en) Three hybrid assay system
WO2002012893A3 (en) Microarrays of functional biomolecules, and uses therefor
WO2001063284A3 (en) Internal standards and controls for multiplexed assay
AU5291600A (en) Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
NO20000086L (en) Chimerically soluble interleukin-6 receptor / ligand protein, and analogues and applications thereof
WO2004070351A3 (en) Protein fragment complementation assays for high-throughput and high-content screening
WO2006124771A3 (en) Multiplex branched-chain dna assays
ZA995566B (en) Ceramic membrane for endothermic reactions.
WO2003045310A8 (en) Digital assay
WO2000004382A8 (en) Arrays of proteins and methods of use thereof
WO2003050295A3 (en) Assays and implements for determining and modulating hsp90 binding activity
AU7181298A (en) Simultaneous humand abo and Rh(D) blood typing or antibody screening by flow ytometry
WO2003022421A3 (en) Microcolumn-platform based array for high-throughput analysis
AUPS159702A0 (en) Molecular detection and assay by magneto-thermal biochip micro-assay
IL135696A0 (en) Analyte assay using particulate labels
WO2001077687A3 (en) Prion-binding peptidic ligands and methods of using same
WO2001018244A3 (en) Selection system
AU2002349344A1 (en) Microfluidic extraction method
AU8388098A (en) Assays for diagnosis of thrombophilic disease
AU1861001A (en) Arrays of glycan molecules (glycoarrays) on the surface of biochips (glycochips)and uses thereof
AU6415000A (en) Indicator peptide for diagnosing and/or predicting cardiovascular and/or endothelial diseases, antibody composition and immunoassay
WO2000033080A3 (en) New assay
AU2002332088A1 (en) Methods and reagents for improved cell-based assays
WO2001034292A3 (en) Macroporous media for biological application prepared by freeze drying

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): CA JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

ENP Entry into the national phase

Ref document number: 2366123

Country of ref document: CA

Ref country code: CA

Ref document number: 2366123

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 612755

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000923395

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000923395

Country of ref document: EP

AK Designated states

Kind code of ref document: C2

Designated state(s): CA JP US

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

COP Corrected version of pamphlet

Free format text: PAGES 3/6-6/6, DRAWINGS, REPLACED BY NEW PAGES 3/6-6/6; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWW Wipo information: withdrawn in national office

Ref document number: 2000923395

Country of ref document: EP