WO2000067549A1 - Seed germination medium - Google Patents
Seed germination medium Download PDFInfo
- Publication number
- WO2000067549A1 WO2000067549A1 PCT/GB2000/001599 GB0001599W WO0067549A1 WO 2000067549 A1 WO2000067549 A1 WO 2000067549A1 GB 0001599 W GB0001599 W GB 0001599W WO 0067549 A1 WO0067549 A1 WO 0067549A1
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- WO
- WIPO (PCT)
- Prior art keywords
- seed
- germination medium
- water
- upper layer
- medium
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/04—Arranging seed on carriers, e.g. on tapes, on cords ; Carrier compositions
- A01C1/042—Tapes, bands or cords
Definitions
- This invention relates to a seed germination medium for encouraging the germination and establishment of seeds, especially in hostile environments, where water, nutrients, climate, chemical contamination and soil conditions may be limiting factors.
- Desertification, salination and soil erosion are significant and increasing problems in many parts of the world. They are typically caused by climatic change, rapidly increasing population, shortage of water and loss of agricultural land to development. This process often places unprecedented pressure for increased productivity from a diminishing and non-renewable land resource. This often leads to contamination of the soil with salts (from contaminated irrigation water, ground water, and fertiliser use) which eventually makes it impossible to grow food or even cover crops. This in turn accelerates soil degradation and leads to desertification.
- salt tolerant species e.g. pistachio, barley and sugar beet
- US-A-5, 189,833 discloses an arrangement in which lawn grass seeds are germinated in athin layer of growing medium sprayed over a mat of non-woven polypropylene lying on top of an impermeable membrane. After a few days of germination, the mat carrying the seed-bearing medium can be lifted, rolled and then spread over an appropriately conditioned and prepared lawn bed. It will be appreciated that such an arrangement is neither intended nor suitable for growing crops in adverse environmental conditions.
- Australian Patent Application AU-A-81394/75 discloses a seed bearing mat comprising upper and lower layers bonded together with a latex adhesive, seeds being bonded between the layers.
- the lower layer is a water permeable material such as straw, coconut fibres, peat moss or wood shavings whilst the upper or covering layer is formed from materials of a finer texture than the lower layer such as coconut fibre dust or sawdust bonded together by latex.
- the seed bearing mat of AU-A-81394/75 is described as being particularly useful in preparing lawns.
- French Patent Application FR-A-2505607 discloses a non-laminar seed germination medium comprising a folded layer of a transparent plastics material such as polyethylene having bonded to the edges of the underside thereof a water-soluble or degradable seed-bearing medium such as paper.
- a transparent plastics material such as polyethylene having bonded to the edges of the underside thereof a water-soluble or degradable seed-bearing medium such as paper.
- the seeds germinate beneath the transparent plastics layer and, as the seedlings grow, they lift the transparent layer away from the water-soluble/degradable medium.
- the transparent layer is gradually unfolded and carried upwards by the growing seedlings and therefore remains in place over the top of the plants to maintain a greenhouse effect.
- French Patent Application FR-A-2440438 discloses a peat-based seed bearing medium comprising a lower layer formed of a water-permeable fibrous material such as cellulose fibres or muslin cloth, an intermediate layer of peat containing seeds and any additives; and optionally an upper layer which is also water-permeable and can be formed from a material such as muslin.
- FR-A-2440438 discloses that the media are envisaged as being economical for large scale use such as plantations in arid regions e.g. deserts, it is also emphasised that an advantage of the peaty medium, compared with polyethylene sheets, is that it allows the penetration of rain water.
- WO-A-96/28010 discloses a seed germination medium in sheet form having upper and lower layers and seeds disposed therebetween.
- the upper and lower layers are preferably formed from a biodegradable paper material.
- the medium is slit longitudinally to enable it to be stretched laterally to form an open lattice.
- a problem with the lattice arrangement disclosed in WO-A-96/28010 is that it has a tendency to dry out too quickly.
- the edges of the lattice openings lift exposing the lower layer of the medium and increasing water loss.
- the lifting of the edges of the lattice results in the seeds being lifted away from the soil which acts to inhibit germination of the seedlings, possibly through build up of ethylene in the medium.
- a further object of the invention is to provide a means of assisting germination and development of seedlings in land contaminated with organic and inorganic materials such as solvents and heavy metals.
- the invention provides a seed-germination medium comprising an upper layer formed from a tissue paper having a weight of about 10 to 30 g m 2 , the tissue paper being coated or impregnated with a waterproofing agent such that the tissue paper is permeable to gases but substantially impermeable to water in liquid form; a lower layer formed from a water- absorbent biodegradable material; seeds being disposed beneath the upper layer; and the upper layer optionally having a plurality of perforations therein to assist disruption of the upper layer to enable seedlings germinating from the seeds to grow therethrough.
- seed germination medium is used above and in the claims appended hereto, the terms “seed matrix” and “seed mat” are also used in the specification, and such terms are intended to be synonymous with “seed germination medium” unless the context indicates otherwise.
- the seed germination media of the invention are of a laminar construction, and typically comprise layers bonded together in such a manner that the layers cannot readily be separated without destroying them.
- the media are distinguished from the media disclosed in FR-A- 2505607 which are of a non-laminar arrangement in which the upper and lower sheets are connected only at their respective edges such that the upper sheet can be lifted away from the lower sheet by the action of the growing plant.
- the upper layer optionally has a plurality of perforations therethrough through which seedlings may grow. Such perforations are preferred when the seedlings are of a more delicate or less robust nature and/or are insufficiently strong to force their way through the upper layer.
- the perforations may be omitted.
- the lower layer can also be provided with a plurality of perforations to enable roots emerging from the seeds to grow therethrough.
- the coated or impregnated tissue paper from which the upper layer is formed should be partially permeable to water vapour but should nevertheless provide some barrier properties with respect to water vapour.
- a major function of the upper layer is to minimise loss of water from the medium through evaporation, and to hold seeds in position when exposed to wind, rain or harsh irrigation.
- tissue paper refers to a lightweight material with relatively poor mechanical strength typically having a weight of approximately 10 to 30 g/m 2 , more usually 15 to 25 g/m 2 , for example from 17g/m 2 to 24 g/m 2 .
- the tissue paper typically has sufficient mechanical strength to enable it to remain intact during manufacture and laying but is preferably sufficiently weak when wet to allow stronger seedlings to grow through.
- perforations may be present in the upper layer to assist the growth of seedlings, particularly where the seedlings are of insufficient strength to burst or "punch" though the tissue.
- the tissue paper can be calendered to give a polished surface and an example of such a calendered paper is sulphite paper available from Kruger Tissue, Church Stretton, Shropshire, UK.
- the tissue paper can be coated with a waterproofing material such as a wax or gas permeable polymeric coating.
- a waterproofing material such as a wax or gas permeable polymeric coating.
- the coating of wate ⁇ roofing agent is relatively thin, for example less than about 10 microns thick.
- the thin layer of waterproofing material is chosen so as to give waterproofing properties for a short period of time until the seedling establishes itself, but contributes relatively little if at all to the strength of the tissue.
- waterproofing polymers include bioerodible polymers and/or polymers which hydrolyse of otherwise break down slowly in the presence of water, air, ultraviolet light or heat, or under the action of microbial attack.
- the tissue paper can be impregnated with a waterproofing material, for example a waterproofing adhesive (such as "Mystolene PS" available from Catomance PLC, UK) which can consist of or contain a waterproofing polymer as hereinbefore defined.
- a waterproofing adhesive such as "Mystolene PS" available from Catomance PLC, UK
- the upper and lower layers are typically bonded together by means of an adhesive.
- the adhesive is typically one which is non-phytotoxic, and most preferably is water-based. Examples of such adhesives are starch, starch derivatives, polyvinyl alcohol (PVA) and ethylene vinyl acetate (EVA), optionally in combination with other adhesives.
- PVA polyvinyl alcohol
- EVA ethylene vinyl acetate
- the adhesive can be applied in such a manner and in such an amount that it permeates the upper layer thereby providing waterproofing properties to the upper layer. Where the adhesive itself in of insufficient waterproofing capability, a waterproofing agent can be added to the adhesive.
- the adhesive is preferably one which is biodegradable or bioerodible.
- the lower layer is an absorbent layer which can be made from a wide range of bio- degradable materials including air-laid paper e.g. an air-laid latex bonded paper, for example a paper formed from fluff pulp and a latex binder; waste or recycled paper; or other fibre-based or waste products.
- air-laid paper e.g. an air-laid latex bonded paper, for example a paper formed from fluff pulp and a latex binder; waste or recycled paper; or other fibre-based or waste products.
- the selection of a suitable absorbent material for the lower layer will be dependent on cost, availability of raw materials and water for irrigation; the soil type, irrigation method, degree of capillarity required and the amount of water which must be retained in the medium to facilitate establishment of the seedling.
- 38 - 150 gm/m 2 air-laid paper is used to form the lower layer, one such form of material being available from Walkisoft of Steinfurt,
- Such air-laid papers typically contain approximately 85% fluff pulp and 15% latex binder, although greater or lesser amounts of binder can be used as desired.
- the lower layer may be relatively non-absorbent, e.g. may be formed from newsprint or other recycled material.
- the lower layer can be a single layer or it can comprise a plurality, e.g. two, three or four, of layers. As with the upper layer, the lower layer can be perforated to assist the penetration of roots emerging from the seeds, although with plants exhibiting strong root growth, this may be unnecessary.
- the upper and/or lower layer can advantageously be coloured or treated to manipulate climatic conditions (e.g. to absorb or reflect heat) and to suppress weed growth below the medium.
- the medium, or at least the surfaces thereof can be provided in a variety of colours to assist soil warming/cooling or to exclude light, so as to suppress weed growth and aid plant establishment.
- the medium may have different colours on its upper and lower surfaces to enhance or reduce the absorption of radiation on one surface and produce the opposite effect on its other surface.
- the perforations in the upper and lower layers are preferably not in register, thereby ensuring that the medium cannot be expanded to form a lattice in the manner disclosed in WO-A-96/28010, and hence preventing weeds growing through.
- the perforations can be slits or holes of any shape but, in the upper layer, preferably they are in the form of slits.
- the upper and lower layers can have different numbers of perforations, and the perforations in the respective layers can be of the same general shape or a different shape. In general, the placement and size of the perforations and cuts will be dependent upon seed size, shape and type.
- a range of optional materials can be disposed beneath the upper layer.
- fertilizers such as zinc, copper, boron, and seaweed extracts
- vitamins such as zinc, copper, boron, and seaweed extracts
- humic acid sea kelp
- sugars such as calcium, copper, boron, and seaweed extracts
- amino acids such as gypsum (calcium sulphate)
- adsorbents such as activated carbon
- the pH-controlling agents can be used to control the acidity of the immediate environment, and agents such as gypsum and activated charcoal can minimise the impact of adverse chemical contaminants and salt in the soil or medium, as well as to improve germination, and/or to aid and accelerate plant establishment.
- Salt binding compounds, buffering and pH regulating compounds and adsorbents typically are applied at between about 20gsm and 200 gsm, more usually 50 to 150 gsm, for example approximately 100 gsm. In the case of the adsorbent, activated carbon, lower concentrations are typically used.
- the buffering and other chemical" or organic agents provide a means of counteracting the impact of adverse chemical contaminants in the soil or medium, as well as improving germination, and/or aiding and accelerating plant establishment.
- lime provides a buffer against low pH
- gypsum provides a means of counteracting high salinity
- clay minerals such as zeolite, kaolinite, calcium bentonite and montmorillonite counteract high levels of fertiliser or chemical contamination in the soil.
- Fertiliser and micronutrients may be added to the medium either by incorporation with the adhesive or applied directly to one or other of the internal surfaces as a spray, granule or dust. If applied at high levels, fertilisers will produce a high level of EC (electro-conductivity) in the medium, which will scorch and kill emerging seedlings. Consequently the rate of application, the type of fertiliser used and the rate of release generally require careful control. Typically, organic based fertilisers are used since such fertilisers release nitrogen more slowly allowing higher rates to be included in the medium without risk of damage to seedlings.
- a typical application would be of a granular, organic-based material at between 10 and 100 grammes per square metre (gsm), more typically 30 - 80 gsm.
- Fertilisers can vary considerably in their potassium, phosphorus and nitrogen contents, but one fertiliser useful in the media of the invention contains 16% nitrogen, 10% phosphorous and 10% potassium plus micro-nutrients.
- Water retaining substances and wetting agents which can be synthetic or natural materials (such as clay minerals, e.g. sodium bentonite (which can be applied at up to 1 OOgsm), or gelatine products, or surfactants such as non-ionic, anionic, cationic and amphoteric surfactants or other wetting adjuvants), can be included to increase the water absorbency of the medium.
- water-retaining substances include polymeric gels such as polyoxyethylene gels (PEO), silica gels and so-called "super absorbents” such as super absorbent acrylic polymers (e.g.
- polyacrylamide which may be added to the medium at between 5 and 100 gsm, preferably 10 to 40 gsm, for example up to about 30 gsm depending on use.
- Polyacrylamide is available for agricultural use from a range of suppliers including, for example, Glowcroft Ltd.
- Polyoxyethylene gels which are particularly preferred in very hostile environments such as highly saline soils, can be obtained from SmartTech Limited of Glasgow, UK, a particular PEO gel being cross-linked polyethylene oxide co-polyurethane hydrogel
- wetting agents these assist in dispersing the water throughout the medium, and allow less absorbent forms of paper or other substrate to be used.
- an advantage of the seed germination media of the invention is that they improve water utilisation. As such, they can be used to reduce the water requirement in situations (such as rice growing) where relatively large amounts of water may normally be required either to enable germination to take place or to suppress weed growth. Furthermore, not only can the seed germination media of the invention advantageously be used to enable growth to take place in hostile terrains, but they can also be used in more temperate conditions, for example in agriculture and horticulture, for example in growing sweetcorn, soya and salad crops.
- Pesticides e.g. herbicides, fungicides, insecticides and nematicides etc.
- pesticides can be incorporated beneath the upper layer and preferably between the layers.
- pesticide/micro- nutrient treated seed can be used, the pesticides can alternatively or additionally be incorporated into, or adsorbed or absorbed onto, the medium. This may reduce the concentration of pesticide (and fertilizer) in direct contact with the seed allowing a broader range of products to be used at higher rates without causing phytotoxicity, reducing the requirement for field applications and dose of active ingredient.
- the seeds and optionally seeding agents such as propagation agents of plant, animal and fungal origin can be bonded or held by physical pressure at a pre-determined density between the layers of the medium.
- the density of seeding will typically depend upon the required plant population and environmental or field factors, for example.
- the seeds can advantageously be treated with pesticides of the type referred to hereinabove.
- each separate layer can be impregnated with or carry a different additive.
- an upper sheet can carry the seeds whilst plant nutrients can be carried by an underlying sheet.
- Biological agents such as bacterial spores and fungal propagation agents (eg mycorrhizae) or other propagation agents may be used to encourage plant establishment in hostile environments and/or provide a means of biodegradation of the upper and lower layers.
- Bacteria and soil fungi can buffer seedlings against soil contaminants " (eg heavy metals) and may be incorporated to attack the upper and/or lower layers to encourage breakdown and the break through of seedlings.
- Typical biological agents may include any one or more of the following:
- Mixtures of bacterial plant growth activators that can be used in the seed germination media of the invention include commercially available products such as “BioPak” (manufactured by Plant Health Care Inc) and “Organica” Plant Growth Activator (manufactured by Organica Ltd).
- bonding of the upper and lower layers can create a trans- laminar effect, which greatly enhances the movement of water across the medium from an irrigation source.
- the use of a semi-permeable layer on top reduces water loss from the absorbent layer, and super absorbents between layers reducing water demand.
- the media of the invention can be provided with and/or used in conjunction with irrigation tubes.
- irrigation tubes can be sandwiched between the upper and lower layers or bonded to one or both of the exterior surfaces of the media.
- the irrigation tubes can be used together with solar pumps, for example, or heat sensitive mechanical pumps which control the distribution of water in accordance with the requirements of the media in a given set of environmental conditions.
- Irrigation tubes useful in the media of the invention can include trickle irrigation tubes which can be polymeric tubes having perforations along their length, ortubes fo ⁇ ned by folding sheets of a polymeric material such as polyethylene and tacking the edges together to form a leaky seam through which water can escape during use.
- the medium of the invention can be provided with reinforcing means for increasing its physical strength.
- the reinforcing means can be made sufficiently robust to enable the use of the medium as a ground anchoring medium in, for example, civil engineering applications, e.g. in the reinforcement of banks.
- the reinforcing means can take the form of a mesh or netting made from a metal or plastics material, or can take the from of a fibrous reinforcement, such as a fibrous mat.
- reinforcing means are present, they are preferably secured to the upper surface of the medium, for example by means of adhesive bonding. By securing the reinforcing means to the upper surface, the medium is held firmly in place.
- irrigation tubes can be placed between the reinforcing means and the medium, the reinforcing means holding the irrigation tubes in place for as long as is required, for example to enable long term maintenance.
- a particular application of such an arrangement is in the stabilization and maintenance of steep or otherwise unstable embankments such as road and rail embankments, or mining spoil or as a covering on waste disposal landfill sites.
- the invention provides a process for stabilising an area of ground (e.g. an embankment, such as a road or rail embankment, or mining spoilage or industrial or domestic waste dump, such as a landfill site) which comprises securing to the ground a seed germination medium as hereinbefore defined.
- an area of ground e.g. an embankment, such as a road or rail embankment, or mining spoilage or industrial or domestic waste dump, such as a landfill site
- Either reinforced or non-reinforced medium can be used to stabilise an area of ground but, in cases such as embankments (e.g. roadside embankments) where the ground may be particularly unstable, the reinforced medium may be preferable.
- embankments e.g. roadside embankments
- the reinforced medium may be preferable.
- the invention provides a method of growing a plant comprising placing a seed germination medium according to the invention on or beneath a soil surface (for example a soil which is saline) and allowing or causing germination of seedlings from the seeds in the medium, such that the seedlings grow through the upper layer of the medium, for example by growing through perforations formed in the upper surface or by disrupting the upper surface to form a hole(s) and growing through the hole(s).
- a soil surface for example a soil which is saline
- the invention provides a process for making the medium as hereinbefore defined, the process comprising the steps of:
- Moisture from adhesives applied during manufacture may initially be absorbed into the lower layer and any super absorbents present and such absorbed water may subsequently be imbibed by the seeds leading to a degree of pre-germination.
- seeds may be pre-treated before manufacture to pre-imbibe moisture by any one or more of a range of techniques, including fermentation and chemical treatment.
- the advantage of pre-germinating the seeds is that it greatly reduces the establishment time of the plant, and reduces the moisture requirement after laying, without detriment to the seed.
- care must be taken (e.g. by seed treatment or moisture control) to avoid fungal and/or microbial attack on the seeds or physiological deterioration.
- the invention provides apparatus for manufacturing a medium as hereinbefore defined, the apparatus comprising first and second support means for holding respectively the first and second webs as hereinbefore defined; a bonding station for bonding the first and second webs together (e.g. by compressing the webs together); means for advancing the first and second webs towards the bonding station; a seed dispenser upstream of the bonding station for depositing seeds onto the first web; optionally adhesive depositing means upstream of the bonding station for applying adhesive to one or both of the webs; and optionally means for perforating one or both of the first and second web prior to or after bonding to form the medium.
- the bonding station can take the form of a pair of rollers, the first and second webs being conveyed through the nip between the rollers, for example after adhesive has been applied to one or both of the webs, the rollers exerting pressure on the webs to bring about bonding therebetween.
- the rollers preferably are relatively soft, or at least have a resilient surface, so that the seeds are not damaged as the webs pass between the rollers.
- bonding can be achieved by applying tension to the finished roll of medium.
- the adhesive can be deposited on the web(s) in the fo ⁇ n of a spray (which can be for example an electrostatic spray), or by means of a roller or brushes.
- adhesive is deposited on the first web prior to depositing seeds on the web so as to assist in retaining the seeds on the web as it is transported towards the bonding station.
- Adhesive is preferably also deposited on the second web upstream of the bonding station.
- the formation of the perforations in each web can be effected by passing the web over or through a cutter.
- the cutter can take the form of a pair of rollers, one having a plurality of cutting elements and the other having a plurality of complimentary recesses for receiving the cutting elements, the web being conveyed through the nip between the rollers.
- cutting of the webs takes place at the bonding station, the bonding station comprising a roller or rollers having laminating/compressing regions and cutting regions.
- the invention provides a cutting and bonding roller for use in the apparatus of the invention, the cutting and bonding roller having one or more (preferably a plurality) axially spaced compression roller elements interspersed with one or more cutting wheels.
- One or more spacer elements may optionally be disposed between adjacent compression roller elements.
- the cutting and bonding roller comprises a drive shaft having mounted thereon a plurality of compression rollers, one or more cutter wheels being disposed between at least one pair of adjacent compression rollers; and a plurality of spacer elements, the spacer elements being interposed between adjacent cutter wheels so as to space the cutter wheels apart.
- the drive shaft can have one or more slots or protrusions or other keying means which cooperate with complimentary formations on the cutter wheels and optionally the spacers so as to hold at least the cutter wheels against relative rotation with respect to the drive shaft.
- the cutter wheels have a plurality of cutting edges spaced (preferably regularly) around their circumferenc ⁇ .
- the cutting edges can be saw toothed or arcuate in form, for example, and can be arranged in either a continuous or discontinuous array.
- the cutter wheel in a discontinuous array, can have a plurality of cutting edges separated by non-cutting edges of a smaller radius than the cutting edges, the cutting and non-cutting edges having substantially concentric radii.
- the cutting edges can have, for example, a substantially sinusoidal form.
- the invention provides an adhesive composition suitable for use in the matrices as hereinbefore defined, the adhesive comprising a water soluble or water dispersible adhesive polymer such as PVA; and one or more (preferably two or more) agents selected from pesticides (for example herbicides, fungicides, insecticides, nematicides), plant micronutrients (such as zinc, copper, boron), organic and inorganic fertilisers, plant growth regulators (e.g. seaweed extract), plant growth promoters and hormones, buffering agents and pH regulators (e.g. lime and sulphur), and salt binding agents such as gypsum (calcium sulphate).
- pesticides for example herbicides, fungicides, insecticides, nematicides
- plant micronutrients such as zinc, copper, boron
- organic and inorganic fertilisers such as plant growth regulators (e.g. seaweed extract), plant growth promoters and hormones, buffering agents and pH regulators (e.g. lime
- Figure 1 is a view from one side of a length of medium according to one embodiment of the invention.
- Figure 2 is a sectional elevation along line I-I in Figure 1 ;
- Figure 3 is a schematic partial cross sectional view illustrating the use of the medium;
- Figure 4 is a schematic side elevation of an apparatus for manufacturing a medium of the type shown in Figures 1 and 2;
- Figure 5a is a side view of a cutter roller of the type used in the apparatus of Figure 4;
- Figure 5b illustrates schematically the arrangement of seed and additive guiding baffles and their spatial relationship with the cutter roller of Figure 5a;
- Figure 6 is a schematic end view illustrating the medium of the invention installed in a soil substrate.
- Figure 7 is a schematic end view illustrating an alternative layout in a soil substrate.
- a medium 2 according to one embodiment of the invention comprises an upper layer 4 and a lower layer 6.
- Upper layer 4 is formed from a lightweight tissue paper which in this embodiment is a 22 grammes per square metre grade of sulphite paper (available from Kruger Tissue Group), which is permeable to gases, and partially permeable to water vapour.
- the surface of the tissue paper layer is treated with a coating of a wax during the manufacture of the germination medium in order to render it impermeable to water in liquid form.
- Lower layer 6 is formed from a 38 - 150 gm/m 2 grade of air-laid paper such as the 38g air-laid paper available from Walkisoft GMBH, Steinfiirt, Germany.
- seeds 8 Disposed between the upper and lower layers 4,6 are seeds 8 which may optionally be coated (e.g. with one or more pesticides).
- Other desirable agents can also be disposed between the layers, examples being super-absorbent substances for increasing the water carrying/retaining capacity of the medium (such as "SwellGel” - available from Glowcroft Ltd, Gloucester, UK.), buffering agents, and beneficial bacteria and fungi as described above.
- the upper and lower layers 4,6 are bonded together by means of an adhesive layer 10 which, in this embodiment, comprises PVA.
- the PVA can contain one or more agents such as pesticides (for example herbicides, fungicides, insecticides, nematicides), plant micronutrients (such as zinc, copper, boron), organic and inorganic fertilisers, plant growth regulators (e.g. seaweed extract), plant growth promoters and hormones, buffering agents and pH regulators (e.g. lime and sulphur), and salt binding agents such as gypsum (calcium sulphate) and microbial species beneficial to plant growth and development and/or which degrade cellulose or other polysaccharides so as to bring about controlled biodegradation of the two paper layers.
- pesticides for example herbicides, fungicides, insecticides, nematicides
- plant micronutrients such as zinc, copper, boron
- organic and inorganic fertilisers such as plant growth regulators (e.g
- the adhesive can be applied in such a manner, and in such an amount, as to soak into the upper layer 4 thereby providing the upper layer with at least temporary waterproofing properties.
- waterproofing additives such as beetle resin can be included in the adhesive.
- the waterproof coating referred to above may be omitted.
- Both the upper and lower layers 4,6 are perforated by an array of slits, the slits in the upper layer 4 being denoted by the number 12 in Figure 1, and the slits in the lower layer 6 not being shown.
- the slits in the upper and lower layers 4,6 are not in register and therefore the medium cannot be opened out into a lattice form in the manner of the medium shown in WO 96/28010.
- the slits provide lines of weakness in the layers which enable the layers to be disrupted by the emerging seedlings so that the seedlings can grow through.
- the slits in the lower layer 6 can be omitted if desired.
- the apparatus comprises a frame 20 to which are rotatably secured a spindle 22, upon which is mounted a roll 24 of the air-laid paper which will constitute the lower layer 6; and a spindle and roll core 26, upon which the finished medium is collected.
- a spindle 22 and 26 Located between the spindles 22 and 26, in sequence, are an adhesive applicator roller 28, guide rollers 29, a drum seeder station 30, a first waterproofing spray station 32, baffles 34, a bonding station 36 comprising a pair of laminating rollers 38, a second waterproofing spray station 40, and optionally a pair of cutter rollers 42 (which can be omitted if perforations are not required on the lower layer, or if the laminating rollers at the bonding station at provided with cutters).
- a seed dispensing hopper 56 and granule applicator 58 are mounted above the first web immediately upstream of the bonding station, and are connected to the baffles 34 by means of chutes 60 and 62 respectively. Seed dispensing hopper 56 may be used as an alternative to the drum seeder station 30, or as an additional means of introducing seeds into the medium.
- a spindle 46 Mounted above the product collecting spindle 26 is a spindle 46 upon which is mounted a roll 48 of the lightweight paper tissue which will constitute the upper layer 4. Disposed between the spindle 46 and the laminating rollers at the bonding station is an adhesive applicator roller 50, guide rollers 51 and adhesive spray head 52 connected to a supply of aqueous adhesive (not shown).
- a web 100 of the air-laid paper is pulled through the machine by means of a powered drive shaft on the product collecting spindle 26.
- the web 100 is drawn along past the adhesive applicator roller 28, such that a thin film of adhesive is applied to the web and then passes under the seed drum 30 where seeds are deposited onto the web 100 from the drum.
- the web then moves 5 along beneath the baffles 34 where granules of a composition containing superabsorbent polymer, other desired additives such as buffers, and beneficial microbial species are dispensed from granule applicator 58.
- the seed dispensing hopper 56 may be used to deposit seeds onto the web.
- the baffles 34 serve to guide the granules and seeds into longitudinal parallel rows.
- the web 102 forming the upper layer of the medium converges with the web 100, which has previously been coated with an adhesive by means of the adhesive roller applicators and the adhesive spray applicator.
- the two webs 100 and 102 are drawn between the rollers 38 and compressed to bond them together. If plain rollers are used at the bonding station
- the two webs will be bonded together across their entire width.
- a roller of the type shown in Figure 5a is used in which case compression and bonding takes place in lines spaced across the width of the webs.
- baffles 34 By configuring the rollers 38, baffles 34 correctly, the seeds and other materials can be introduced onto the web in rows such that compression and lamination takes place either side of each row. This prevents any damage to the seeds as a result of 0 the action of the rollers, and also ensures that a more secure bond is formed between the two webs at the locations where there are no seeds or other materials.
- the roller shown in Figure 5a cuts slits into the medium at the same time as laminating the two webs together, and thereby removes the need for a separate cutting or slitting station 40.
- a roller having no cutting edges may be used, and a separate cutting station 40 provided. 5 After the bonding stage and subsequent winding, some water from the adhesive may still remain in the paper, held by the more absorbent lower web 100. Allowing the web to retain some water can lead to partial germination of the seed or initiation of the germination step. This can be advantageous in certain circumstances and, for example, can help to bring about more rapid establishment of the plant when the medium is put to use.
- the webs may optionally be conveyed through an oven, drier or air- knife prior to winding onto the roll. Removal of the water prior to storage assists in maintaining a prolonged shelf life for the medium.
- the above process is preferably controlled by means of a central processing unit 70 linked to the various motors used to drive the spindles, compression rollers and seed, adhesive and additive dispensing means thereby ensuring greater consistency in the end product.
- the size of the rolls of seed germination medium formed according to the foregoing process will depend upon the raw materials, the intended method of laying (by agricultural machinery or by hand), and the area intended to be covered. However, purely by way of example, the rolls can be up to 1.2 metres wide, 4200 metres in length, and may cover up to about 0.5 hectares or more.
- the upper and optionally the lower webs are slit by cutter rollers.
- one or both of the webs can be pre-cut.
- the lower layer 6 of the medium need not be cut at all, the rooting strength of the germinating seedling being sufficient to force a way through the lower surface of the medium.
- the apparatus illustrated and in particular the positioning of the adhesive rollers and sprays, and the waterproofing spray heads, is sufficiently versatile that it could equally be set up in the reverse configuration should the need arise.
- the seeds can applied using the drum seeder 30.
- the seed hopper/plate seeder 56 or a similar device could be used to broadcast seed on to the web.
- Other biological agents such as bacterial spores may be added in the same way.
- the seeds can be laid into rows using the baffles as guides, but they need not be, but instead could be spread evenly over the entire surface of the web.
- Each cutter roller comprises a central spindle or drive shaft 200 on which is mounted an array of compression rollers (d), narrow spacers (c) cutter wheels (a) and wide spacers (b).
- the compression rollers, cutter wheels and spacers are held against rotation on the spindle by means of key slots (not shown) which engage a spline (not shown) on the spindle.
- each pair of adjacent compression rollers (d) has a pair of cutter wheels (a) arranged therebetween, the cutter wheels (a) being spaced from each other by means of a large spacer (b) and from the compression rollers (d) by means of narrow spacers (c).
- FIG 5b the alignment of the baffles with regard to the various elements of the bonding rollers is shown.
- the gaps between the baffles are aligned with the wide spacers (b) such that seeds and other materials are deposited onto the web between the regions of the webs that are subjected to compression by the compression rollers (d).
- the cutter wheels (a) By positioning the cutter wheels (a) such that they are at the edges of the unbonded regions of the medium, lines of weakness are formed that enable the developing seedlings more easily to rupture and grow through the upper layer.
- the seed germination medium of the invention can be laid using existing agricultural or horticultural equipment, for example the machinery typically used for laying polyethylene sheeting, or crop mulching equipment.
- the soil or ground can be prepared in advance of the laying to produce a relatively smooth clod free bed.
- Furrows F are then opened up on either side of the medium and the medium is partially buried as shown in Figure 6 and 7. Burying the edges E of the medium helps to hold it in place against the action of, for example, winds.
- Trickle irrigation tubes T (for example of the "Drip Tape" type - available from Wright Rain Limited, Ringwood, UK) can be incorporated at laying or the medium may be wetted utilizing overhead, ditch or flood irrigation.
- the medium As much of the medium as possible should be covered to assist establishment and reduce water consumption although the medium can be laid on the surface if necessary and an appropriate securing system used. Once wetted, the medium will retain moisture to allow the seeds to germinate. Ideally little or no further moisture should be applied to encourage rapid deep rooting and self sustainability. However, this may not be practical in all situations and additional water may be required.
- a form of flood irrigation could be used by laying the medium into a depression in the ground, which may formed manually or by the equipment used to lay the medium, and allowing or causing water to run over the surface of the upper layer.
- the advantage of the medium of the present invention is that it provides a protected local environment which is favourable to the germination of seeds and the subsequent development of seedlings.
- the medium has a substantially reduced water demand compared to conventional overhead and trickle irrigation systems, and can be applied to land using existing plastics/mulch laying equipment.
- a further benefit of the medium is its compatibility with existing trickle, overhead, trench and flood irrigation systems. Once the medium has been laid, there is a minimal requirement for additional irrigation. If desired, however, a trickle irrigation system can be incorporated into the medium at manufacture. Further substantial advantages are the ease of manufacture of the medium and its long shelf life. Moreover, the medium can be used in both hostile and normal agricultural situations.
- a number of samples were made up using 50 gsm (grammes per square metre weight) air- laid paper for the lower layer and sulphited tissue paper as the upper layer. The materials were cut to 10cm 2 squares before applying seeds and various additives and then laminating.
- a starch-based adhesive incorporating a wate ⁇ roofing agent obtained from National Starch NS Ref.0720185 was used to laminate the two layers together.
- Various additives were placed in two discrete rows 2cm wide with a 1 cm gap between rows and at the edges on to the lower absorbent layer. Adhesive was applied to the upper layer which was then laminated to the lower layer using a profiled roller of the type shown in Figure 5a.
- the bacteria present in the plant growth stimulator have a beneficial effect on the establishment of the seedlings and that, moreover, they cause beneficial rotting of the upper and lower layers.
- damage to the seedlings can result if the conditions are kept too wet.
- the concentration (rate) of application of the PGS is probably too high and preferably should be lowered to about 1 gsm;
- composition for use in the seed germination media of the invention is as follows:
- Bacteria Plant growth stimulator 1 gsm
- the pu ⁇ ose of the fine grade vermiculite is to improve aeration and reduce the risk of anaerobic conditions developing in the medium.
- a seed germination matrix of the invention is that which has an electrical conductivity of greater than 4 dS/m.
- Experiments were also carried out to evaluate the interaction of various microbiological additives with the grass species.
- the following salts (calcium chloride, magnesium chloride and sodium chloride) were applied to a sterilised growing substrate (John Innes Seed Compost) at rates which were sufficient to increase the electrical conductivity to 12 dS/m (medium salinity) and 22 dS/m (high salinity) following the method of Rowell (Soil Science, Methods and Applications, Publ. Longman Scientific & Technical, 1994).
- the untreated substrate was used as the control and had an electrical conductivity of 2.5 dS/m.
- the measurement of electrical conductivity was performed as follows: 20 grammes of soil and 100 ml of substrate were shaken for one hour after which the suspensions were allowed to settle and the electrical conductivity of the supernatants was measured using either a DiST 3, or DiST 4 handheld conductivity meter. These values were converted to those representative of the saturated extract, following the method of Rowell (1994).
- the substrates and salts (where required) were mixed together using a compost mixer to ensure an even distribution of salts throughout the substrate.
- Table 3 Grass species listed in order of tolerance to saline conditions
- a range of soil bacterial and fungal inoculants were inco ⁇ orated into the matrix for evaluation.
- a proprietary mix of inoculants (Symbio Ltd, Great Bookham, UK) was selected for the trial.
- the mix included vesicular-arbuscular mycorrhizae, pseudomonas, and bacillus species and a range of other phosphorous solubilising and nitrogen fixing bacteria with biostimulants.
- the fungi, bacteria and additives used are generally referred to as 'bugs'.
- Samples (0.15 sq. m) were made by hand from a super-absorbent air-laid paper (Walkisoft) forming a lower layer and perforated sulphite paper (Kruger Tissue, Church Stretton, Shropshire, UK) forming the upper layer.
- An aluminium frame was used to subdivide the lower layer into 16 strips, each 30 cm in length, and 2 rows were allocated to each cultivar.
- Seeds, fertilisers (Westland Specialist Feed-All), polyacrylamide gel (“Swell Gel” - Glowcroft Limited)) were applied to all treatments and the microbiological additives were applied at the equivalent field rate to half of the treatments. The application rates were as follows: fertiliser (20 g/sq.m), polyacrylamide gel (30 g/sq.m) and the microbiological additives at 50 g/sq.m.
- a PVA adhesive (Mystolene, Colomance PLC, Welwyn Garden City, UK) was applied to the upper layer which was then bonded to the lower layer using a laminating process which bound the layers and encapsulated the seed and additives.
- a wax emulsion was sprayed onto the exposed surface of the upper layer to provide wate ⁇ roofing.
- the gravel served two pu ⁇ oses: firstly, to prevent the substrate slumping into the base of the trays and, secondly, to act as a reservoir.
- the substrate (8 litres) was then placed over the water-filled gravel, the matrix was laid over the substrate, and half of the matrix was buried.
- the treatments are summarised in Table 4. There were three trays for each treatment and one for each control. All the trays were placed in a heated and lit greenhouse. The average daily temperature was 20 °C and the lights were on for a period of 12 hours each day (08:00 - 20:00).providing a light intensity of 61 micro mols/sq.m/second.
- Soil salinity is a major and increasing limitation to agricultural production. It is caused by the accumulation of salts in the rooting zone. At higher levels (above 6 dS/m) the presence of salt affects many crops and prevents all but tolerant species from being established.
- One way to overcome this problem is to leach the salts out of the soil profile. Continual irrigation will prevent salts being drawn back to the surface but if insufficient water is applied salinity will increase as salts are drawn up to the surface. Without effective management these soils may be lost from agricultural production thereby beginning the process of desertification.
- Plant growth at the saline trial site was impaired by a combination of these factors.
- the matrix of the invention inco ⁇ orates various buffers and absorbents to allow plants to be established in hostile conditions with minimum water requirement.
- Microbiological additives bacteria and mycorrhizae
- Plant growth is enhanced in certain environments where plant roots have been inoculated with these organisms and inoculation is more successful where seeds, bacteria and mycorrhizae are placed together in a protective environment.
- the plots were selected on the basis that they had saline soils that were a major problem to the farmer.
- the majority of soils at Freemans Reach are clay loams overlying clay that is mottled, a characteristic of poorly drained soils.
- the topsoil has a weak structure that sets hard when dry.
- the soils have a background salinity of at least 8 dS/m. Soil salinity has developed through effluent seepage from a nearby intensive livestock units and from groundwater which is inherently saline. The former has also led to excessive levels of phosphorous in the soils within the catchment and consequently an antagonistic reaction to some of the soil bacteria was expected.
- Tetila ryegrass (Lolium perenne) 25 Kentucky bluegrass (Poa pretensis) 50
- grasses and clover species were kept separate and each species was laid in two adjacent rows, 30 cm apart, onto air-laid paper.
- Additives were applied evenly over the seeded area, with the exception of A-100, a bacterial inoculant, that was applied at logarithmic scale in order to assess its effect on seed germination and to determine a suitable rate of application.
- Perforated and calendered tissue paper was then attached, using PVA adhesive, and laminated to produce a seed mat.
- Salinity was typically 8 dS/m with one small area at 3.2 dS/m. The area with low salinity was at the top of the gentle slope where increased leaching of the salts was possible. Consequently this was included in the study as a control area. A lower lying area was selected for undertaking the bulk of the trials where higher levels were consistent.
- the pre-prepared sample rolls were laid onto the top of raised beds on 9 March 2000 with direct seeded controls in the high and low saline areas of the site.
- the top of the bed was levelled and soil was moved to the outer edge.
- the mats were laid on the levelled surface and soil was drawn onto the mat to provide a covering of between 10 and 20 mm.
- the control treatments were direct sown on the beds at the end of the seed mat treatments. Seeds were sown into rows; these were then covered with the same depth of soil to mimic the treatment plots as closely as possible. Irrigation of the plots was unnecessary as the area suffered from unprecedented levels of rainfall following laying. This resulted in structural damage of the topsoil and subsequent capping. Poor aeration ensued which provided a very hostile environment.
- Seed mats were prepared on the basis of the above observations with PEO Hydrogel, calcium bentonite, zeolite and with and without the inclusion of A-100 bacterial inoculant, applied evenly at 15 g sq.m. Grass and clover species were mixed before application in rows. Otherwise the manufacturing protocol remained unchanged.
- Table 10 Summary of results from 10m plots Site Treatment Salinity Dry matter dS/m S/m 2
- Burying of the matrix has no detrimental effect on germination and protected emerging seedlings from the effect of heavy rainfall.
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/959,559 US6681521B1 (en) | 1999-05-05 | 2000-05-05 | Seed germination medium |
AU47667/00A AU757176B2 (en) | 1999-05-05 | 2000-05-05 | Seed germination medium |
AT00929658T ATE270492T1 (en) | 1999-05-05 | 2000-05-05 | SEED GERMINATION CARRIER |
CA002372811A CA2372811C (en) | 1999-05-05 | 2000-05-05 | Seed germination medium |
DE60012006T DE60012006T2 (en) | 1999-05-05 | 2000-05-05 | A seed germination medium |
EP00929658A EP1175139B1 (en) | 1999-05-05 | 2000-05-05 | Seed germination medium |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9910346.7 | 1999-05-05 | ||
GBGB9910346.7A GB9910346D0 (en) | 1999-05-05 | 1999-05-05 | Seed germination medium |
Publications (1)
Publication Number | Publication Date |
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WO2000067549A1 true WO2000067549A1 (en) | 2000-11-16 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2000/001599 WO2000067549A1 (en) | 1999-05-05 | 2000-05-05 | Seed germination medium |
Country Status (10)
Country | Link |
---|---|
US (1) | US6681521B1 (en) |
EP (1) | EP1175139B1 (en) |
AT (1) | ATE270492T1 (en) |
AU (1) | AU757176B2 (en) |
CA (1) | CA2372811C (en) |
DE (1) | DE60012006T2 (en) |
ES (1) | ES2226837T3 (en) |
GB (1) | GB9910346D0 (en) |
PT (1) | PT1175139E (en) |
WO (1) | WO2000067549A1 (en) |
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Also Published As
Publication number | Publication date |
---|---|
CA2372811A1 (en) | 2000-11-16 |
AU757176B2 (en) | 2003-02-06 |
EP1175139A1 (en) | 2002-01-30 |
ATE270492T1 (en) | 2004-07-15 |
DE60012006T2 (en) | 2005-08-04 |
US6681521B1 (en) | 2004-01-27 |
PT1175139E (en) | 2005-01-31 |
AU4766700A (en) | 2000-11-21 |
CA2372811C (en) | 2008-11-04 |
DE60012006D1 (en) | 2004-08-12 |
EP1175139B1 (en) | 2004-07-07 |
GB9910346D0 (en) | 1999-06-30 |
ES2226837T3 (en) | 2005-04-01 |
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