WO2001014853A1 - System for processing and imaging of samples - Google Patents
System for processing and imaging of samples Download PDFInfo
- Publication number
- WO2001014853A1 WO2001014853A1 PCT/US2000/021627 US0021627W WO0114853A1 WO 2001014853 A1 WO2001014853 A1 WO 2001014853A1 US 0021627 W US0021627 W US 0021627W WO 0114853 A1 WO0114853 A1 WO 0114853A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- block
- container
- imaging
- tissue
- Prior art date
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 44
- 238000012545 processing Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 claims description 26
- 238000004590 computer program Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 86
- 210000001519 tissue Anatomy 0.000 description 42
- 239000000463 material Substances 0.000 description 16
- 239000000126 substance Substances 0.000 description 10
- 238000000386 microscopy Methods 0.000 description 8
- 230000008595 infiltration Effects 0.000 description 7
- 238000001764 infiltration Methods 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 239000000975 dye Substances 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 239000000834 fixative Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012188 paraffin wax Substances 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 3
- -1 for example Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000008096 xylene Substances 0.000 description 3
- 208000035404 Autolysis Diseases 0.000 description 2
- 206010057248 Cell death Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000000339 bright-field microscopy Methods 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 238000007388 punch biopsy Methods 0.000 description 2
- 230000007281 self degradation Effects 0.000 description 2
- 230000028043 self proteolysis Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000721701 Lynx Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000013188 needle biopsy Methods 0.000 description 1
- 239000003605 opacifier Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920000307 polymer substrate Polymers 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000003777 tissue processing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001519161A JP2003507734A (en) | 1999-08-25 | 2000-08-08 | System for sample processing and imaging |
AU65301/00A AU6530100A (en) | 1999-08-25 | 2000-08-08 | System for processing and imaging of samples |
EP00952641A EP1216408A1 (en) | 1999-08-25 | 2000-08-08 | System for processing and imaging of samples |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38308599A | 1999-08-25 | 1999-08-25 | |
US09/383,085 | 1999-08-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001014853A1 true WO2001014853A1 (en) | 2001-03-01 |
Family
ID=23511655
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/021627 WO2001014853A1 (en) | 1999-08-25 | 2000-08-08 | System for processing and imaging of samples |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1216408A1 (en) |
JP (1) | JP2003507734A (en) |
CN (1) | CN1372638A (en) |
AU (1) | AU6530100A (en) |
TW (1) | TW463042B (en) |
WO (1) | WO2001014853A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7372985B2 (en) | 2003-08-15 | 2008-05-13 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
WO2010056945A2 (en) * | 2008-11-13 | 2010-05-20 | Shalon Ventures, Inc. | Methods and systems for tissue processing and imaging |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4960330A (en) * | 1985-07-16 | 1990-10-02 | Kerschmann Russell L | Image recording apparatus |
US5991028A (en) * | 1991-02-22 | 1999-11-23 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for cell classification |
-
2000
- 2000-08-08 AU AU65301/00A patent/AU6530100A/en not_active Abandoned
- 2000-08-08 EP EP00952641A patent/EP1216408A1/en not_active Withdrawn
- 2000-08-08 WO PCT/US2000/021627 patent/WO2001014853A1/en not_active Application Discontinuation
- 2000-08-08 JP JP2001519161A patent/JP2003507734A/en active Pending
- 2000-08-08 CN CN00811944A patent/CN1372638A/en active Pending
- 2000-10-09 TW TW089117055A patent/TW463042B/en not_active IP Right Cessation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4960330A (en) * | 1985-07-16 | 1990-10-02 | Kerschmann Russell L | Image recording apparatus |
US5991028A (en) * | 1991-02-22 | 1999-11-23 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for cell classification |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7372985B2 (en) | 2003-08-15 | 2008-05-13 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
US9589173B2 (en) | 2003-08-15 | 2017-03-07 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
WO2010056945A2 (en) * | 2008-11-13 | 2010-05-20 | Shalon Ventures, Inc. | Methods and systems for tissue processing and imaging |
WO2010056945A3 (en) * | 2008-11-13 | 2010-08-12 | Shalon Ventures, Inc. | Methods and systems for tissue processing and imaging |
Also Published As
Publication number | Publication date |
---|---|
JP2003507734A (en) | 2003-02-25 |
CN1372638A (en) | 2002-10-02 |
TW463042B (en) | 2001-11-11 |
EP1216408A1 (en) | 2002-06-26 |
AU6530100A (en) | 2001-03-19 |
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