WO2001016374A3 - Methods and reagents for in situ amplification - Google Patents

Methods and reagents for in situ amplification Download PDF

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Publication number
WO2001016374A3
WO2001016374A3 PCT/US2000/023734 US0023734W WO0116374A3 WO 2001016374 A3 WO2001016374 A3 WO 2001016374A3 US 0023734 W US0023734 W US 0023734W WO 0116374 A3 WO0116374 A3 WO 0116374A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
reagents
amplification
situ
situ amplification
Prior art date
Application number
PCT/US2000/023734
Other languages
French (fr)
Other versions
WO2001016374A2 (en
Inventor
Yuexia Li
Kathy Lynn Hickman
Eun Mi Lee
Gregory J Hurteau
Original Assignee
Akzo Nobel Nv
Yuexia Li
Kathy Lynn Hickman
Eun Mi Lee
Gregory J Hurteau
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel Nv, Yuexia Li, Kathy Lynn Hickman, Eun Mi Lee, Gregory J Hurteau filed Critical Akzo Nobel Nv
Priority to EP00957907A priority Critical patent/EP1212460B1/en
Priority to JP2001520919A priority patent/JP4608162B2/en
Priority to DE60038029T priority patent/DE60038029T2/en
Publication of WO2001016374A2 publication Critical patent/WO2001016374A2/en
Publication of WO2001016374A3 publication Critical patent/WO2001016374A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

The present invention provides in situ amplification methods for the detection of a target nucleic acid molecule. The methods use a molecule termed 'molecular tape' to retain the amplified target nucleic acid molecule within a cell upon amplification. The molecular tape, which may be used with a variety of amplification methods, improves the in situ detection of an amplified target molecule.
PCT/US2000/023734 1999-09-01 2000-08-30 Methods and reagents for in situ amplification WO2001016374A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00957907A EP1212460B1 (en) 1999-09-01 2000-08-30 Methods and reagents for in situ amplification
JP2001520919A JP4608162B2 (en) 1999-09-01 2000-08-30 Methods and reagents for in situ amplification
DE60038029T DE60038029T2 (en) 1999-09-01 2000-08-30 METHODS AND REAGENTS FOR SITU AMPLIFICATION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/387,455 1999-09-01
US09/387,455 US6187567B1 (en) 1999-09-01 1999-09-01 Methods and reagents for in situ amplification

Publications (2)

Publication Number Publication Date
WO2001016374A2 WO2001016374A2 (en) 2001-03-08
WO2001016374A3 true WO2001016374A3 (en) 2001-11-01

Family

ID=23529941

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/023734 WO2001016374A2 (en) 1999-09-01 2000-08-30 Methods and reagents for in situ amplification

Country Status (6)

Country Link
US (1) US6187567B1 (en)
EP (1) EP1212460B1 (en)
JP (1) JP4608162B2 (en)
AT (1) ATE386140T1 (en)
DE (1) DE60038029T2 (en)
WO (1) WO2001016374A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040146918A1 (en) * 2000-02-18 2004-07-29 Weiner Michael L. Hybrid nucleic acid assembly
CA2513646C (en) * 2003-01-24 2013-09-17 Michael R. Emmert-Buck Target activated microtransfer
US7695752B2 (en) * 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
US7957466B2 (en) * 2005-09-16 2011-06-07 Sony Corporation Adaptive area of influence filter for moving object boundaries
US20080299622A1 (en) * 2007-02-07 2008-12-04 Paulson Bradley A Starch Hydrolysis Using Phytase with an Alpha Amylase
US20110147837A1 (en) * 2009-12-23 2011-06-23 Hafez Walid M Dual work function gate structures

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5538871A (en) * 1991-07-23 1996-07-23 Hoffmann-La Roche Inc. In situ polymerase chain reaction
WO1997007241A1 (en) * 1995-08-14 1997-02-27 University College London Nucleic acid amplification and in-situ detection
WO1997011196A2 (en) * 1995-09-21 1997-03-27 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5863726A (en) * 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5021335A (en) 1988-06-17 1991-06-04 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5225326A (en) 1988-08-31 1993-07-06 Research Development Foundation One step in situ hybridization assay
US5506105A (en) 1991-12-10 1996-04-09 Dade International Inc. In situ assay of amplified intracellular mRNA targets
US5589333A (en) 1992-02-03 1996-12-31 Thomas Jefferson University In situ polymerase chain reaction
US5521061A (en) 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538871A (en) * 1991-07-23 1996-07-23 Hoffmann-La Roche Inc. In situ polymerase chain reaction
US5863726A (en) * 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
WO1997007241A1 (en) * 1995-08-14 1997-02-27 University College London Nucleic acid amplification and in-situ detection
WO1997011196A2 (en) * 1995-09-21 1997-03-27 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAASE A T ET AL: "AMPLIFICATION AND DETECTION OF LENTIVIRAL DNA INSIDE CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 87, no. 13, 1 July 1990 (1990-07-01), pages 4971 - 4975, XP000371711, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
ATE386140T1 (en) 2008-03-15
JP4608162B2 (en) 2011-01-05
US6187567B1 (en) 2001-02-13
EP1212460B1 (en) 2008-02-13
WO2001016374A2 (en) 2001-03-08
DE60038029D1 (en) 2008-03-27
JP2003519471A (en) 2003-06-24
DE60038029T2 (en) 2009-02-05
EP1212460A2 (en) 2002-06-12

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